CN108113943B - Oral care method for children - Google Patents
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Abstract
The invention discloses a children oral care method, which comprises the step of gargling for 5-15 minutes by using the children oral care solution at least once every day, and the children oral care solution is continuously used for four weeks or more. The oral cavity nursing method can clean the oral cavity and the teeth, has good relieving effect on common oral cavity problems such as toothache, gingival bleeding, oral ulcer, gingivitis, periodontitis, sore throat, oral odor and the like, can maintain normal oral cavity microbial flora, ensures oral cavity health, and finally achieves the effects of removing oral odor, preventing decayed teeth, promoting tooth calcification, firming teeth, nourishing gums and healthy periodontium.
Description
Technical Field
The invention relates to an oral care method, in particular to an oral care method for children.
Background
The mouth and throat are the channels for food intake and respiration, and the indispensable way for the dietary water to enter the human body, and are closely related to the healthy survival of the human body. Therefore, the oral cavity problems are easily caused by the factors of the health of the human body, bacterial and viral infection, air pollution, excessive sound, food stimulation by hot and spicy substances, and the like, and if the treatment is not performed, the treatment is performed by mistake, and the treatment is performed by mistake, the oral cavity problems are easily developed into periodontitis and the like.
In daily life, people are used to clean teeth and oral cavities by toothpaste, but certain people or certain occasions always have no condition or cannot brush teeth by toothpaste for various reasons, such as after a meal, before a client and before and after tooth treatment. Especially for children who do not brush their teeth, it is very important to maintain oral health through an effective oral care method.
The oral health care of children not only comprises the congenital development condition of the oral cavity, which is closely related to the health condition of the whole body, but also comprises acquired factors, namely good eating habits, good oral hygiene habits, regular oral examinations, prevention of accidental injuries of the oral cavity and the like. At present, the children oral care method mainly achieves the effects of cleaning and eliminating oral odor by gargling the oral cavity with mouth wash, oral care solution and the like, and also has the effects of removing dental plaque and killing bacteria in the oral cavity with the oral care solution. However, oral care solutions, mouth washes and the like sold in the market at present have different qualities. It is necessary to research a new oral care method for children and develop a new oral care solution.
Disclosure of Invention
The invention provides a children oral care method. The invention adopts the following technical scheme:
a method of oral care for a child: the children oral care solution is used for gargling for 5-15 minutes at least once daily, and is continuously used for four weeks or more.
A method of oral care for a child: the children oral care solution is gargled for 5-15 minutes by 15-30mL of the children oral care solution at least once a day and used each time, and is continuously used for four weeks or more, wherein the children oral care solution comprises the following components in parts by weight: 0.5-5 parts of plant extract, 0.3-0.9 part of xylitol, 0.05-0.5 part of vitamin C, 0.05-0.2 part of ethanol, 0.001-0.003 part of lauroyl arginine ethyl ester hydrochloride, 0.005-0.02 part of microcrystalline cellulose, 0.001-0.01 part of glucosamine, 0.001-0.01 part of allantoin, 0.005-0.03 part of solubilizer and 65-85 parts of water.
A method of oral care for a child: the children oral care solution is gargled for 5-15 minutes by 15-30mL of the children oral care solution at least once a day and used each time, and is continuously used for four weeks or more, wherein the children oral care solution comprises the following components in parts by weight: 0.5-5 parts of plant extract, 0.01-0.1 part of marine sipunculus extract, 0.3-0.9 part of xylitol, 0.05-0.5 part of vitamin C, 0.05-0.2 part of ethanol, 0.001-0.003 part of lauroyl arginine ethyl ester hydrochloride, 0.005-0.02 part of microcrystalline cellulose, 0.001-0.01 part of glucosamine, 0.001-0.01 part of allantoin, 0.005-0.03 part of solubilizer and 65-85 parts of water.
The invention also provides a children oral care solution which comprises the following components in parts by weight: 0.5-5 parts of plant extract, 0.3-0.9 part of xylitol, 0.05-0.5 part of vitamin C, 0.05-0.2 part of ethanol, 0.001-0.003 part of lauroyl arginine ethyl ester hydrochloride, 0.005-0.02 part of microcrystalline cellulose, 0.001-0.01 part of glucosamine, 0.001-0.01 part of allantoin, 0.005-0.03 part of solubilizer and 65-85 parts of water.
The invention also provides a children oral care solution which comprises the following components in parts by weight: 0.5-5 parts of plant extract, 0.01-0.1 part of marine sipunculus extract, 0.3-0.9 part of xylitol, 0.05-0.5 part of vitamin C, 0.05-0.2 part of ethanol, 0.001-0.003 part of lauroyl arginine ethyl ester hydrochloride, 0.005-0.02 part of microcrystalline cellulose, 0.001-0.01 part of glucosamine, 0.001-0.01 part of allantoin, 0.005-0.03 part of solubilizer and 65-85 parts of water.
Preferably, the preparation method of the plant extract of the present invention is as follows: pulverizing radix Ophiopogonis, cortex Cinnamomi Japonici, herba Andrographitis, herba Violae, and herba Violae, respectively, sieving with 20-50 mesh sieve, taking 6-15g of sieved radix Ophiopogonis, 2-10g of cortex Cinnamomi Japonici, 1-5g of herba Andrographitis, and 0.5-2.5g of herba Violae, adding into 30-90g of ethanol water solution with mass fraction of 65-85% and preheated to 30-40 deg.C, ultrasonic extracting under the conditions of power of 200 ion and 300W and frequency of 40-50KHz for 20-60 min, centrifuging at the rotation speed of 5000 ion and 8000 rpm for 25-45 min, taking the upper layer liquid after centrifugation, sieving with 100 mesh sieve and 500 mesh sieve, retaining filtrate, evaporating and concentrating the filtrate to obtain extract with relative density of 1.31-1.33 at 50 deg.C, drying the extract at 55-65 deg.C and 0.04-0.06MPa, obtaining plant extract with water content of 0.8-1.2% on dry basis.
Further preferably, the preparation method of the plant extract is as follows: pulverizing radix Ophiopogonis, cortex Cinnamomi Japonici, herba Andrographitis, and herba Violae, respectively, sieving with 20 mesh sieve, collecting sieved radix Ophiopogonis 9-9.5g, cortex Cinnamomi Japonici 6-6.5g, herba Andrographitis 2-2.3g, herba Andrographitis 3-3.5g, and herba Violae 1-1.2g, adding into 85-90g ethanol water solution with mass fraction of 80-85% and preheated to 35-38 deg.C, ultrasonic extracting under the conditions of 200W power and 40KHz frequency for 30-40 min, centrifuging at 7000-.
Most preferably, the plant extract is prepared as follows: pulverizing radix Ophiopogonis, cortex Cinnamomi Japonici, herba Andrographitis, herba Violae, and herba Violae, respectively, sieving with 20 mesh sieve, adding sieved radix Ophiopogonis 9g, cortex Cinnamomi Japonici 6g, herba Andrographitis 2g, herba Andrographitis 3g, and herba Violae 1g into ethanol water solution with mass fraction of 85% and preheated to 35 deg.C 90g, ultrasonically extracting at power of 200W and frequency of 40KHz for 30 min, centrifuging at rotation speed of 8000 rpm for 30 min, collecting supernatant, sieving with 300 mesh sieve, retaining filtrate, evaporating and concentrating the filtrate to obtain extract with relative density of 1.31 at 50 deg.C, and drying at 55 deg.C and 0.04MPa to obtain plant extract with dry basis water content of 0.8%.
Preferably, the preparation method of the asterias amurensis extract comprises the following steps: 4mol/L sodium chloride aqueous solution, 6mmol/L EDTA aqueous solution, 0.2mol/L Na2HPO3Aqueous solution and 0.2mol/L NaH2PO3Uniformly mixing the aqueous solution according to the volume ratio of (80-100): 3-7): 4-8 to obtain an extracting agent, cleaning the marine astrus, draining off the surface moisture, taking 250-plus 260g of the marine astrus homogenate into 1000-plus 1200g of the extracting agent preheated to 35-40 ℃, performing ultrasonic extraction for 45-60 minutes under the conditions of 200-plus 250W of power and 40-50KHz of frequency, then centrifuging for 30-45 minutes at the rotating speed of 8000-plus 10000 rpm, taking the centrifuged upper layer liquid, sieving through 300-plus 500 meshes to obtain primary filtrate and primary filter residue, adding the primary filtrate into the primary filtrate320g of ethanol with the concentration of 300-.
Preferably, the preparation method of the asterias amurensis extract comprises the following steps: 4mol/L sodium chloride aqueous solution, 6mmol/L EDTA aqueous solution, 0.2mol/L Na2HPO3Aqueous solution and 0.2mol/L NaH2PO3Uniformly mixing the water solution according to the volume ratio of 100:100:3:8 to obtain an extracting agent, cleaning the marine sipunculus nudus, draining surface water, taking 250g of the marine sipunculus nudus homogenate into 1000g of the extracting agent preheated to 35 ℃, performing ultrasonic extraction for 45 minutes under the conditions of 200W of power and 40KHz of frequency, then centrifuging for 45 minutes at the rotating speed of 10000 revolutions per minute, taking the centrifuged upper layer liquid, sieving by a 300-mesh sieve to obtain primary filtrate and primary filter residue, adding 300g of ethanol into the primary filtrate, preserving for 3 hours at the temperature of 4 ℃, sieving by a 300-mesh sieve to obtain secondary filtrate and secondary filter residue, adding 200g of ethanol into the secondary filtrate, preserving for 3 hours at the temperature of 3 ℃, sieving by a 300-mesh sieve to obtain tertiary filtrate and tertiary filter residue, adding 100g of ethanol into the tertiary filtrate, preserving for 2 hours at the temperature of 5 ℃, sieving by a 300-mesh sieve to obtain four filtrate and filter residue, merging the secondary filter residue, filtering, And (4) carrying out freeze drying on the third filter residue and the fourth filter residue to obtain the asterias amurensis extract with the water content of 2.5% on a dry basis.
Preferably, the freeze-drying condition parameters are: the pre-freezing temperature is set to-40 to-35 ℃, the sublimation temperature is set to 3-5 ℃, the analysis temperature is set to 25-35 ℃, and the vacuum degree is 10-15 Pa.
Preferably, the solubilizer is a mixture of caprylic capric glyceride and gum arabic, and the mass ratio of the caprylic capric glyceride to the gum arabic is (3-5): (1-2).
Further preferably, the solubilizer is a mixture of caprylic capric glyceride and gum arabic, and the mass ratio of the caprylic capric glyceride to the gum arabic is 3: 2.
the invention also provides a preparation method of the children oral care solution, which comprises the following steps: weighing the raw materials in parts by weight, and uniformly mixing.
The invention also provides application of the children oral care solution in children oral care.
The oral cavity nursing method can clean the oral cavity and the teeth, has good relieving effect on common oral cavity problems such as toothache, gingival bleeding, oral ulcer, gingivitis, periodontitis, sore throat, oral odor and the like, can maintain normal oral cavity microbial flora, ensures oral cavity health, and finally achieves the effects of removing oral odor, preventing decayed teeth, promoting tooth calcification, firming teeth, nourishing gums and healthy periodontium.
On the basis of the common knowledge in the field, the above preferred conditions can be combined randomly to obtain the preferred embodiments of the invention.
Detailed Description
The invention is further illustrated by the following examples, which are not intended to limit the scope of the invention. The experimental methods without specifying specific conditions in the following examples were selected according to the conventional methods and conditions, or according to the commercial instructions.
In the following examples, the main raw materials and equipment used were as follows:
andrographis paniculata (Burm.f.) Nees.) is dried whole plant on the ground. Has effects of clearing away heat and toxic materials, cooling blood, and relieving swelling.
Dried whole plant of Androsace umbellata (Lour.) Merr of Primulaceae is used as Androsace umbellata. Has the effects of clearing away heat and toxic materials, and relieving swelling and pain. Can be used for treating tonsillitis, pharyngolaryngitis, acute contagious conjunctivitis, traumatic injury, and sore throat.
Herba Violae is dried whole plant of Viola yedoensis Makino of Violaceae. Clearing away heat and toxic material, cooling and relieving swelling. Belonging to the category of heat-clearing herbs.
Radix Ophiopogonis, dried root tuber of Ophiopogon japonicus (Thunb.) ker-Gawl.) belonging to Liliaceae, has effects of protecting cardiovascular system, enhancing anoxia tolerance, prolonging survival time of anoxia at normal pressure, enhancing immunity, lowering blood sugar, and delaying aging.
Cinnamon, which is the dry bark of cinnamomum japonicum Sieb, belonging to Lauraceae, has the effects of warming the middle-jiao to dispel cold, and regulating qi to alleviate pain.
Caprylic capric acid glycerides, CAS number: 65381-09-1.
The marine sipunculus nudus is also called as sea ginseng, the famous sipunculus nudus is also called as nudus sipunculus nudus, commonly called as sandworm. Not only has delicious taste, but also is a high-grade nutritional health food with low sugar, low fat, high protein and rich in zinc, iron, calcium, phosphorus, iodine and other mineral substances. Besides being eaten, the marine sipunculus nudus is regarded as a 'marine medicine' from ancient times, and the traditional Chinese medicine considers that the marine sipunculus nudus is sweet, salty and cold in nature and has the effects of clearing lung and tonifying deficiency, and nourishing yin and reducing fire.
Lauroyl arginine ethyl ester hydrochloride, CAS No.: 60372-77-2.
Xylitol, CAS No.: 87-99-0.
Vitamin C, CAS No.: 50-81-7.
Ethanol, CAS No.: 64-17-5 and the purity is 99.5 percent.
Microcrystalline cellulose, CAS No.: 9004-36-8, a polymerization degree of 3000-.
Glucosamine, CAS No.: 3416-24-8.
Allantoin, CAS number: 97-59-6.
1, 2-propanediol, CAS No.: 57-55-6.
Gum arabic, CAS No.: 9000-01-5, and food grade.
The invention is further illustrated by the following examples, in which the parts are by weight unless otherwise specified.
Example 1
A method of oral care for a child: 20mL of children oral care solution is used for gargling for 5 minutes in three times every morning, noon and evening, and the children oral care solution is continuously used for four weeks and comprises the following components in parts by weight: 1.5 parts of plant extract, 0.6 part of xylitol, 0.25 part of vitamin C, 0.1 part of ethanol, 0.001 part of lauroyl arginine ethyl ester hydrochloride, 0.007 part of microcrystalline cellulose, 0.004 part of glucosamine, 0.002 part of allantoin, 0.009 part of solubilizer and 80 parts of water.
The solubilizer is 1, 2-propylene glycol.
The preparation method of the plant extract comprises the following steps: respectively crushing radix ophiopogonis, cassia bark, common andrographis herb and philippine violet herb, sieving the crushed radix ophiopogonis 15g, cassia bark 10g, common andrographis herb 1g and philippine violet herb 0.5g, adding the sieved radix ophiopogonis 15g, cassia bark 10g, common andrographis herb 1g, common viola herb 1g and philippine violet herb 0.5g into ethanol water solution with the mass fraction of 90g and preheated to 35 ℃, ultrasonically extracting the mixture for 30 minutes under the conditions of 200W power and 40KHz frequency, centrifuging the mixture for 30 minutes at the rotating speed of 8000 rpm, taking the centrifuged upper layer liquid, sieving the mixture through a 300-mesh sieve, retaining filtrate, evaporating and concentrating the filtrate to extract with the relative density of 1.31 measured at 50 ℃, and drying the extract under the conditions of 55 ℃ and 0.04MPa to obtain the.
The preparation method of the children oral care solution comprises the following steps: weighing the raw materials in parts by weight, and uniformly mixing.
Example 2
The same as example 1, except that the plant extract of example 2 was prepared as follows: pulverizing radix Ophiopogonis, cortex Cinnamomi Japonici, herba Andrographitis, herba Violae, and herba Violae, respectively, sieving with 20 mesh sieve, adding sieved radix Ophiopogonis 9g, cortex Cinnamomi Japonici 6g, herba Andrographitis 2g, herba Andrographitis 3g, and herba Violae 1g into ethanol water solution with mass fraction of 85% and preheated to 35 deg.C 90g, ultrasonically extracting at power of 200W and frequency of 40KHz for 30 min, centrifuging at rotation speed of 8000 rpm for 30 min, collecting supernatant, sieving with 300 mesh sieve, retaining filtrate, evaporating and concentrating the filtrate to obtain extract with relative density of 1.31 at 50 deg.C, and drying at 55 deg.C and 0.04MPa to obtain plant extract with dry basis water content of 0.8%.
Example 3
The same as example 1, except that the plant extract of example 3 was prepared as follows: pulverizing radix Ophiopogonis, cortex Cinnamomi Japonici, herba Andrographitis, herba Violae, and herba Violae, respectively, sieving with 20 mesh sieve, taking 6g of sieved radix Ophiopogonis, 2g of cortex Cinnamomi Japonici, 5g of herba Andrographitis, and 2.5g of herba Violae, adding into 90g of ethanol water solution with mass fraction of 85% and preheated to 35 deg.C, ultrasonically extracting at 200W and 40KHz for 30 min, centrifuging at 8000 rpm for 30 min, taking the upper layer liquid after centrifugation, sieving with 300 mesh sieve, retaining the filtrate, evaporating and concentrating the filtrate to obtain extract with relative density of 1.31 at 50 deg.C, drying the extract at 55 deg.C and 0.04MPa to obtain plant extract with dry basis water content of 0.8%.
Example 4
A method of oral care for a child: 20mL of children oral care solution is used for gargling for 5 minutes in three times every morning, noon and evening, and the children oral care solution is continuously used for four weeks and comprises the following components in parts by weight: 1.5 parts of plant extract, 0.05 part of marine sipunculus extract, 0.6 part of xylitol, 0.25 part of vitamin C, 0.1 part of ethanol, 0.001 part of lauroyl arginine ethyl ester hydrochloride, 0.007 part of microcrystalline cellulose, 0.004 part of glucosamine, 0.002 part of allantoin, 0.009 part of solubilizer and 80 parts of water.
The solubilizer is 1, 2-propylene glycol.
The preparation method of the plant extract comprises the following steps: pulverizing radix Ophiopogonis, cortex Cinnamomi Japonici, herba Andrographitis, herba Violae, and herba Violae, respectively, sieving with 20 mesh sieve, adding sieved radix Ophiopogonis 9g, cortex Cinnamomi Japonici 6g, herba Andrographitis 2g, herba Andrographitis 3g, and herba Violae 1g into ethanol water solution with mass fraction of 85% and preheated to 35 deg.C 90g, ultrasonically extracting at power of 200W and frequency of 40KHz for 30 min, centrifuging at rotation speed of 8000 rpm for 30 min, collecting supernatant, sieving with 300 mesh sieve, retaining filtrate, evaporating and concentrating the filtrate to obtain extract with relative density of 1.31 at 50 deg.C, and drying at 55 deg.C and 0.04MPa to obtain plant extract with dry basis water content of 0.8%.
The preparation method of the marine sipunculus extract comprises the following steps: 4mol/L sodium chloride aqueous solution, 6mmol/L EDTA aqueous solution, 0.2mol/L Na2HPO3Aqueous solution and 0.2mol/L NaH2PO3Uniformly mixing the water solution according to the volume ratio of 100:100:3:8 to obtain an extracting agent, cleaning the marine sipunculus nudus, draining surface water, taking 250g of the marine sipunculus nudus homogenate into 1000g of the extracting agent preheated to 35 ℃, performing ultrasonic extraction for 45 minutes under the conditions of 200W of power and 40KHz of frequency, then centrifuging for 45 minutes at the rotating speed of 10000 revolutions per minute, taking the centrifuged upper layer liquid, sieving by a 300-mesh sieve to obtain primary filtrate and primary filter residue, adding 300g of ethanol into the primary filtrate, preserving for 3 hours at the temperature of 4 ℃, sieving by a 300-mesh sieve to obtain secondary filtrate and secondary filter residue, adding 200g of ethanol into the secondary filtrate, preserving for 3 hours at the temperature of 3 ℃, sieving by a 300-mesh sieve to obtain tertiary filtrate and tertiary filter residue, adding 100g of ethanol into the tertiary filtrate, preserving for 2 hours at the temperature of 5 ℃, sieving by a 300-mesh sieve to obtain four filtrate and filter residue, merging the secondary filter residue, filtering, And (4) carrying out freeze drying on the third filter residue and the fourth filter residue to obtain the asterias amurensis extract with the water content of 2.5% on a dry basis. The condition parameters of the freeze drying are as follows: the prefreezing temperature was set at-35 deg.C, the sublimation temperature was set at 5 deg.C, the analysis temperature was set at 35 deg.C, and the degree of vacuum was 15 Pa.
The preparation method of the children oral care solution comprises the following steps: weighing the raw materials in parts by weight, and uniformly mixing.
Example 5
Essentially the same as example 4, except that: the preparation method of the asterias amurensis extract in the embodiment 5 comprises the following steps: 4mol/L sodium chloride aqueous solution, 6mmol/L EDTA aqueous solution, 0.2mol/L Na2HPO3Aqueous solution and 0.2mol/L NaH2PO3Mixing the water solution at a volume ratio of 100:80:4:6 to obtain an extractant, cleaning the marine sipunculus nudus, draining off surface water, placing 250g of the marine sipunculus nudus homogenate in 1000g of the extractant preheated to 35 ℃, performing ultrasonic extraction for 45 minutes under the conditions of 200W of power and 40KHz of frequency, and then centrifuging for 45 minutes at a rotating speed of 10000 r/minTaking the centrifuged upper layer liquid, sieving the upper layer liquid by a 300-mesh sieve to obtain a primary filtrate and a primary filter residue, adding 300g of ethanol into the primary filtrate, preserving the primary filtrate for 3 hours at the temperature of 4 ℃, sieving the primary filtrate by a 300-mesh sieve to obtain a secondary filtrate and a secondary filter residue, adding 200g of ethanol into the secondary filtrate, preserving the secondary filtrate and the secondary filter residue for 3 hours at the temperature of 3 ℃, sieving the secondary filtrate by a 300-mesh sieve to obtain a tertiary filtrate and a tertiary filter residue, adding 100g of ethanol into the tertiary filtrate, preserving the tertiary filtrate for 2 hours at the temperature of 5 ℃, sieving the tertiary filter residue and the quaternary filter residue to obtain a quaternary filtrate and a quaternary filter residue, combining the secondary filter residue, the tertiary filter residue and the quaternary filter residue, and freeze. The condition parameters of the freeze drying are as follows: the prefreezing temperature was set at-35 deg.C, the sublimation temperature was set at 5 deg.C, the analysis temperature was set at 35 deg.C, and the degree of vacuum was 15 Pa.
Example 6
Essentially the same as example 4, except that: the preparation method of the asterias amurensis extract in the embodiment 6 comprises the following steps: 4mol/L sodium chloride aqueous solution, 6mmol/L EDTA aqueous solution, 0.2mol/L Na2HPO3Aqueous solution and 0.2mol/L NaH2PO3Uniformly mixing the water solution according to a volume ratio of 80:100:7:4 to obtain an extracting agent, cleaning the marine sipunculus nudus, draining surface water, taking 250g of the marine sipunculus nudus homogenate to 1000g of the extracting agent preheated to 35 ℃, performing ultrasonic extraction for 45 minutes under the conditions of 200W of power and 40KHz of frequency, then centrifuging for 45 minutes at a rotating speed of 10000 revolutions per minute, taking the centrifuged upper layer liquid, sieving by a 300-mesh sieve to obtain primary filtrate and primary filter residue, adding 300g of ethanol into the primary filtrate, preserving for 3 hours at a temperature of 4 ℃, sieving by a 300-mesh sieve to obtain secondary filtrate and secondary filter residue, adding 200g of ethanol into the secondary filtrate, preserving for 3 hours at a temperature of 3 ℃, sieving by a 300-mesh sieve to obtain tertiary filtrate and tertiary filter residue, adding 100g of ethanol into the tertiary filtrate, preserving for 2 hours at a temperature of 5 ℃, sieving by a 300-mesh sieve to obtain four filtrate and filter residue, merging the secondary filter residue, filtering, And (4) carrying out freeze drying on the third filter residue and the fourth filter residue to obtain the asterias amurensis extract with the water content of 2.5% on a dry basis. The condition parameters of the freeze drying are as follows: pre-freezing temperature of-35 deg.C, sublimation temperature of 5 deg.C, analysis temperature of 35 deg.CThe degree of hollowness is 15 Pa.
Example 7
The method is basically the same as example 4, except that the solubilizer in the embodiment 7 is caprylic/capric glyceride.
Example 8
Essentially the same as example 4, except that the solubilizing agent described in this example 8 was gum arabic.
Example 9
Basically the same as example 4, except that the solubilizer in this example 9 is a mixture of caprylocaproyl caprin and gum arabic, and the mass ratio of the caprylocaproyl caprin to the gum arabic is 3: 2.
comparative example 1
A method of oral care for a child: 20mL of children oral care solution is used for gargling for 5 minutes in three times every morning, noon and evening, and the children oral care solution is continuously used for four weeks and comprises the following components in parts by weight: 0.6 part of xylitol, 0.25 part of vitamin C, 0.1 part of ethanol, 0.001 part of lauroyl arginine ethyl ester hydrochloride, 0.007 part of microcrystalline cellulose, 0.004 part of glucosamine, 0.002 part of allantoin, 0.009 part of solubilizer and 80 parts of water.
The solubilizer is 1, 2-propylene glycol.
The preparation method of the children oral care solution comprises the following steps: weighing the raw materials in parts by weight, and uniformly mixing.
Comparative example 2
A method of oral care for a child: 20mL of children oral care solution is used for gargling for 5 minutes in three times every morning, noon and evening, and the children oral care solution is continuously used for four weeks and comprises the following components in parts by weight: 1.5 parts of plant extract, 0.6 part of xylitol, 0.25 part of vitamin C, 0.1 part of ethanol, 0.001 part of lauroyl arginine ethyl ester hydrochloride, 0.007 part of microcrystalline cellulose, 0.004 part of glucosamine, 0.002 part of allantoin, 0.009 part of solubilizer and 80 parts of water.
The solubilizer is 1, 2-propylene glycol.
The preparation method of the plant extract comprises the following steps: pulverizing radix Ophiopogonis, cortex Cinnamomi Japonici, herba Andrographitis, herba Violae, and herba Violae, respectively, sieving with 20 mesh sieve, adding sieved radix Ophiopogonis 4g, cortex Cinnamomi Japonici 1g, herba Andrographitis 7g, and herba Violae 0.5g into ethanol water solution with mass fraction of 90g of 85% preheated to 35 deg.C, ultrasonically extracting at power of 200W and frequency of 40KHz for 30 min, centrifuging at rotation speed of 8000 rpm for 30 min, collecting the upper layer liquid, sieving with 300 mesh sieve, retaining the filtrate, evaporating and concentrating the filtrate to obtain extract with relative density of 1.31 at 50 deg.C, and drying at 55 deg.C and 0.04MPa to obtain plant extract with dry basis water content of 0.8%.
The preparation method of the children oral care solution comprises the following steps: weighing the raw materials in parts by weight, and uniformly mixing.
Comparative example 3
A method of oral care for a child: 20mL of children oral care solution is used for gargling for 5 minutes in three times every morning, noon and evening, and the children oral care solution is continuously used for four weeks and comprises the following components in parts by weight: 1.5 parts of plant extract, 0.6 part of xylitol, 0.25 part of vitamin C, 0.1 part of ethanol, 0.001 part of lauroyl arginine ethyl ester hydrochloride, 0.007 part of microcrystalline cellulose, 0.004 part of glucosamine, 0.002 part of allantoin, 0.009 part of solubilizer and 80 parts of water.
The solubilizer is 1, 2-propylene glycol.
The preparation method of the plant extract comprises the following steps: pulverizing radix Ophiopogonis, cortex Cinnamomi Japonici, herba Andrographitis, herba Violae, and herba Violae, respectively, sieving with 20 mesh sieve, adding sieved radix Ophiopogonis 20g, cortex Cinnamomi Japonici 15g, herba Andrographitis 0.5g, herba Andrographitis 0.2g, and herba Violae 0.1g into ethanol water solution with mass fraction of 90g of 85% preheated to 35 deg.C, ultrasonically extracting at power of 200W and frequency of 40KHz for 30 min, centrifuging at rotation speed of 8000 rpm for 30 min, collecting upper layer liquid, sieving with 300 mesh sieve, retaining filtrate, evaporating and concentrating the filtrate to obtain extract with relative density of 1.31 at 50 deg.C, and drying at 55 deg.C and 0.04MPa to obtain plant extract with dry basis water content of 0.8%.
The preparation method of the children oral care solution comprises the following steps: weighing the raw materials in parts by weight, and uniformly mixing.
Effect test 1Gingival disease alleviation effect test
120 children with age of 8-10 years of gingival inflammation patients are selected. The oral care method for children of examples 1-9 and comparative examples 1-3 were used for treatment in 12 groups of 10 persons: 20mL of the children oral care solution is used for gargling for 5 minutes three times in the morning, at noon and at night, and the curative effect is observed after the children oral care solution is continuously used for four weeks. SPSS16.0 statistical software is applied in the experiment, and x is adopted as technical data2The significance level α was tested to 0.05. Patients with gingival inflammation were evaluated using a modified Loe-Silness Gingivitis Index (GI) method: 0: no gingivitis; 1: slight gingivitis, slight change of gum color and texture, no bleeding during probing; 2: moderate gingivitis, bright, red and swollen gums, hyperplasia, palpation bleeding; 3: severe gingivitis, marked redness and swelling of the gums, hyperplasia, and spontaneous bleeding. Comparisons between the results of the groups were statistically significant.
Table 1: gum disease treatment effect test table
Effect test 2Test of bacteriostatic Effect
Test strains: streptococcus mutans (ATCC 25923), and Candida albicans (ATCC 76615). The test method comprises the following steps: respectively activating each strain, picking a small amount of thallus with inoculating loop, placing in 10mL sterile water, and performing gradient dilution to obtain 106-107CFU/mL of bacterial suspension for later use. The filter paper is punched into a filter paper sheet with the diameter of 6mm by a puncher, and the filter paper sheet is put into 10mL of the children oral care solution used by the invention for soaking for 24h after being sterilized by dry heat at 160 ℃, and sterile water is used as a reference. Sucking 100 μ L of the prepared bacterial suspension, and uniformly coating on corresponding culture dishAnd (3) cloth. Draining the filter paper sheet with sterile forceps to remove excessive liquid, placing into bacteria-containing culture dishes with 2 sheets each, placing the culture dishes inoculated with Candida albicans into a 37 deg.C incubator, and incubating for 24 hr; putting the culture dish inoculated with the streptococcus mutans into an anaerobic bag, incubating for 48h at 37 ℃, performing 3 times of repeated experiments on each bacterium, respectively measuring the diameter (in a cross shape) of each inhibition zone in two perpendicular directions by using a vernier caliper, and averaging to judge the inhibition effect.
Table 2: bacteriostatic ring diameter test table, unit: mm is
As can be seen from Table 1, the gingivitis index of the patient is obviously reduced when the oral care method for children disclosed by the invention is continuously used for nursing the oral cavity, and the oral care method for children disclosed by the invention can effectively relieve the gingival disease. Examples 1-3 the oral care solutions for children used contain plant extracts that are preferred for their better gingival relief in children by optimizing the amounts of different plants. The oral care solution for children used in the invention also contains the marine sipunculus nudus extract, and the marine sipunculus nudus extract is found to have a good oral care effect and an obvious effect of relieving children's gingival symptoms. Examples 7-9 the solubilizing agent for children's oral care solutions is preferred to promote solubility and stability of the antibacterial and anti-inflammatory components of the solution. As can be seen from Table 2, the children oral care method has good antibacterial and bactericidal effects on oral care, and has remarkable bactericidal effects on common streptococcus mutans and candida albicans.
The foregoing detailed description of the preferred embodiments of the invention has been presented. It should be understood that numerous modifications and variations could be devised by those skilled in the art in light of the present teachings without departing from the inventive concepts. Therefore, the technical solutions available to those skilled in the art through logic analysis, reasoning and limited experiments based on the prior art according to the concept of the present invention should be within the scope of protection defined by the claims.
Claims (2)
1. The children oral care solution is characterized by comprising the following components in parts by weight: 0.5-5 parts of plant extract, 0.01-0.1 part of marine sipunculus extract, 0.3-0.9 part of xylitol, 0.05-0.5 part of vitamin C, 0.05-0.2 part of ethanol, 0.001-0.003 part of lauroyl arginine ethyl ester hydrochloride, 0.005-0.02 part of microcrystalline cellulose, 0.001-0.01 part of glucosamine, 0.001-0.01 part of allantoin, 0.005-0.03 part of solubilizer and 65-85 parts of water;
the solubilizer is a mixture of caprylic capric glyceride and gum arabic, and the mass ratio of the caprylic capric glyceride to the gum arabic is (3-5): (1-2);
the preparation method of the marine sipunculus extract comprises the following steps: 4mol/L sodium chloride aqueous solution, 6mmol/L EDTA aqueous solution, 0.2mol/L Na2HPO3Aqueous solution and 0.2mol/L NaH2PO3Uniformly mixing the aqueous solution according to the volume ratio of (80-100): 3-7): 4-8 to obtain an extracting agent, cleaning the marine astrus, draining the surface moisture, taking 250-plus 260g of the marine astrus homogenate into 1000-plus 1200g of the extracting agent preheated to 35-40 ℃, performing ultrasonic extraction for 45-60 minutes under the conditions of 200-plus 250W of power and 40-50KHz of frequency, then centrifuging for 30-45 minutes at the rotating speed of 8000-plus 10000 rpm, taking the centrifuged upper layer liquid, sieving through 300-plus 500 meshes to obtain a primary filtrate and a primary filter residue, adding 300-plus 320g of ethanol into the primary filtrate, preserving for 3-3.5 hours at the temperature of 3-4 ℃, sieving through 300-plus 500 meshes to obtain a secondary filtrate and a secondary filter residue, adding 230g of 200-plus ethanol into the secondary filtrate, storing for 3-3.5 hours at the temperature of 2-3 ℃, sieving with a 300-fold sieve with 500 meshes to obtain a third filtrate and a third filter residue, adding 110g of 100-fold ethanol into the third filtrate, storing for 2-2.5 hours at the temperature of 4-5 ℃, sieving with a 300-fold sieve with 500 meshes to obtain a fourth filtrate and a fourth filter residue, combining the second filter residue, the third filter residue and the fourth filter residue, and freeze-drying to obtain the marine asteroid extract with the dry basis water content of 2.5-3%;
the preparation method of the plant extract comprises the following steps: pulverizing radix Ophiopogonis, cortex Cinnamomi Japonici, herba Andrographitis, herba Violae, and herba Violae, respectively, sieving with 20-50 mesh sieve, taking 6-15g of sieved radix Ophiopogonis, 2-10g of cortex Cinnamomi Japonici, 1-5g of herba Andrographitis, and 0.5-2.5g of herba Violae, adding into 30-90g of ethanol water solution with mass fraction of 65-85% and preheated to 30-40 deg.C, ultrasonic extracting under the conditions of power of 200 ion and 300W and frequency of 40-50KHz for 20-60 min, centrifuging at the rotation speed of 5000 ion and 8000 rpm for 25-45 min, taking the upper layer liquid after centrifugation, sieving with 100 mesh sieve and 500 mesh sieve, retaining filtrate, evaporating and concentrating the filtrate to obtain extract with relative density of 1.31-1.33 at 50 deg.C, drying the extract at 55-65 deg.C and 0.04-0.06MPa, obtaining plant extract with water content of 0.8-1.2% on dry basis.
2. The children oral care solution according to claim 1, wherein the raw materials are weighed according to parts by weight and mixed uniformly.
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