CN108029278A - A kind of method that Picea Mongolica seed germination rate is improved using gibberellin - Google Patents
A kind of method that Picea Mongolica seed germination rate is improved using gibberellin Download PDFInfo
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- CN108029278A CN108029278A CN201711297858.9A CN201711297858A CN108029278A CN 108029278 A CN108029278 A CN 108029278A CN 201711297858 A CN201711297858 A CN 201711297858A CN 108029278 A CN108029278 A CN 108029278A
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- seed
- vernalization
- river sand
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
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- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- General Health & Medical Sciences (AREA)
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- Agronomy & Crop Science (AREA)
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- Pretreatment Of Seeds And Plants (AREA)
Abstract
The present invention provides a kind of method that Picea Mongolica seed germination rate is improved using gibberellin, comprise the following steps：1) sterile Picea Mongolica seed is soaked into 20~28h using the Gibberellins solution of 0.3~0.8mg/L and obtains pretreatment seed；2) by step 1) the pretreatment seed with pre-processing river sand with volume ratio 1：Obtain vernalization system after the ratio mixing of (2~5), under the conditions of the vernalization system is stacked at 10~15 DEG C vernalization 7~10 days obtain the seed that shows money or valuables one carries unintentionally.The method of the invention can effectively break seed dormancy, improve the germination rate of seed, and it is neat to emerge, and makes Picea Mongolica seed germination rate up to more than 90%.
Description
Technical field
The invention belongs to Picea Mongolica technical field of cultivation, and in particular to one kind improves Picea Mongolica seed using gibberellin
The method of germination rate.
Background technology
Picea Mongolica category aiphyllium, up to 30m, the diameter of a cross-section of a tree trunk 1.3 meters above the ground is about 60cm；The tree crown of Picea Mongolica is in grey blue-green, bark
Flakey, light, compared with shade tolerant, resists cold, drought-resistant, barren-resistant.Picea Mongolica is rare rare tree, and it is muddy to originate in China Inner Mongol
Starch 1500 gram sand ground east.The lateral root system of Picea Mongolica is more flourishing, and root long is 3 times of trunk, and root system sprawling interweaves, deep-rooted,
Fragmentary fine sand can be gathered, have special-effect to checking winds and fixing drifting sand.
Stratification method is used after the seed treatment of Picea Mongolica nursery at present, typically seed disinfection, since seed is stopped
The reason for dormancy, seed show money or valuables one carries unintentionally irregular, and germination rate is low, cause after planting emergence rate it is low, and irregular, later stage seedling raising process of emerging
In survival rate of seedlings it is low.
The content of the invention
In view of this, it is an object of the invention to provide a kind of side that Picea Mongolica seed germination rate is improved using gibberellin
Method, so as to improve the survival rate of seedlings of later stage nursery.
In order to realize foregoing invention purpose, the present invention provides following technical scheme：One kind improves sand ground cloud using gibberellin
The method of China fir seed germination rate, comprises the following steps：1) using the sterile sand ground cloud of Gibberellins solution seed soaking of 0.3~0.8mg/L
20~28h of China fir seed obtains pretreatment seed；2) by step 1) the pretreatment seed with pre-processing river sand with volume ratio 1：(2
~5) ratio mixing obtains vernalization system, and the vernalization system is stacked vernalization in 7~10 days in 10~15 DEG C obtains the kind that shows money or valuables one carries unintentionally
Son.
Preferably, the Gibberellins solution concentration described in step 1) is 0.4~0.6mg/L.
Preferably, the seed soaking time described in step 1) is 22~26h.
Preferably, step 2) the pretreatment river sand is process washing successively, carbendazim is sterilized, dried, Gibberellins solution
The river sand soaked.
Preferably, the pretreatment seed and the volume ratio for pre-processing river sand are 1：(3~4).
Preferably, the mass concentration of the carbendazim is 0.45~0.55%.
Preferably, the concentration for soaking the Gibberellins solution of river sand is 0.4~0.6mg/L.
Preferably, sterile Picea Mongolica seed is through KMnO by Picea Mongolica seed described in step 1)4Solution immersion 1~
3h is obtained.
Preferably, the KMnO4The mass concentration of solution is 0.4~0.6%.
Preferably, described in step 2) in pregermination procedure the vernalization system is stirred every 20~28h once.The present invention's
Beneficial effect：The method of the invention handles sterile Picea Mongolica seed using Gibberellins solution, is accelerated using Exogenous gibberellic acid
The hydrolysis of starch in seed endosperm, promotes seed to sprout, can effectively break seed dormancy, improve the germination rate of seed, adopt at the same time
It is neat with sand storage method vernalization, emergence.
Further, the present invention is soaked rear river sand and mixed with pretreatment seed using Gibberellins solution stacks vernalization, makes sand
Ground dragon spruce seed germination rate is up to more than 90%.
Embodiment
The present invention provides a kind of method that Picea Mongolica seed germination rate is improved using gibberellin, comprise the following steps：
1) pretreatment seed is obtained using 20~28h of the Gibberellins solution sterile Picea Mongolica seed of seed soaking of 0.3~0.8mg/L；2) will
Step 1) the pretreatment seed is with pretreatment river sand with volume ratio 1：The ratio mixing of (2~5) obtains vernalization system, by described in
Vernalization system stacks vernalization in 7~10 days in 10~15 DEG C and obtains the seed that shows money or valuables one carries unintentionally.
The present invention preferably further includes cleaning for Picea Mongolica seed before the sterile Picea Mongolica Seeds preprocess；
Described clean preferably uses water float glass process, and specifically by spare Picea Mongolica seed immersion, 15~25min, removing are floating in water
Empty not plump seed on the water surface, pulls full seed out and carries out disinfection and obtain sterile Picea Mongolica seed.It is described in the present invention
Sterile Picea Mongolica seed is preferably by Picea Mongolica seed through KMnO4Solution soaks 1~3h and obtains；The KMnO4The quality of solution
Concentration is preferably 0.4~0.6%, and more preferably 0.5%；The time of the immersion is preferably 2h.The KMnO4Solution
Preferably carry out draining after immersion and obtain sterile Picea Mongolica seed.
In the present invention, 20~28h of Gibberellins solution seed soaking by sterile Picea Mongolica seed using 0.3~0.8mg/L
Obtain pretreatment seed.The concentration of heretofore described Gibberellins solution is preferably 0.4~0.6mg/L, more preferably
0.5mg/L；The seed soaking time is preferably 22~26h, more preferably 24h.The gibberellin can pass through in the present invention
A series of Ca on biological effect excitation seed cell films+The activity of transport protein, promotes cell to absorb Ca by consuming ATP+, and
Ca+It can induce the synthesis of seed alpha-amylase again, so as to accelerate the hydrolysis of starch in seed endosperm, promote seed to sprout.Therefore,
Gibberellin, which has, releases seed dormancy, and can stimulate the physiological action for having terminated dormancy kind growth of the embryo, is remarkably improved seed
Germination percentage and germinating energy.
After the seed soaking, the currently preferred seed by after the seed soaking is pulled out, is drained away the water.
The present invention is after pretreatment seed is obtained, by the pretreatment seed with pretreatment river sand with volume ratio 1：(2~5)
Ratio mixing obtain vernalization system.
The pretreatment river sand is to be sterilized successively by washing, carbendazim, dry and soaked with Gibberellins solution in the present invention
Wet river sand.The carbendazim mass concentration is preferably 0.45~0.55% in the present invention, and more preferably 0.5%；
Heretofore described carbendazim sterilization method is not particularly limited, using this area conventional method.It is described in the present invention
Preferably river sand is dried after carbendazim disinfection；Gibberellins solution of the present invention is soaked in river sand specific implementation process preferably
Gibberellins solution is sprayed into the river sand dried, the concentration of the Gibberellins solution of the sprinkling is preferably 0.3~0.8mg/
L, more preferably 0.4~0.6mg/L, most preferably 0.5mg/L.The present invention does not have the fountain height of the Gibberellins solution
Particular determination, so as to soaking subject to river sand；The pretreatment river sand obtained after Gibberellins solution is specifically sprayed in embodiment
State for " hold it is agglomerating, one pine i.e. dissipate ".The purpose for pre-processing river sand using gibberellin in the present invention is to give Picea Mongolica kind
Son persistently provides higher concentration Exogenous gibberellic acid environment, to improve the speed of seed breaking dormancy.
The pretreatment seed is preferably 1 with pre-processing the volume ratio of river sand in the present invention:(3~4), more preferably
For 1:3.
After the present invention obtains vernalization system, the vernalization system is stacked into vernalization 7~10 days in 10~15 DEG C, is showed money or valuables one carries unintentionally
Seed.In the present invention, the temperature of the vernalization is preferably 9~14 DEG C；The time of the vernalization is preferably 8~9 days；Institute
To state in pregermination procedure and vernalization system is stirred, the time interval stirred is preferably 20~28h, and more preferably 22
~26h；Most preferably 24h.Heretofore described vernalization system, which is preferably fitted into woven bag, stacks.
Germination accelerating method advantage of the present invention is insulation, moisturizing, can release seed dormancy rapidly, saves seed, effectively
Ground improves the bud ratio of seedling, shortens the seeding stage, and emergence is neat, strengthens seedling quality, and then reduces seedling cost and disease
Occur.Test result indicates that sandy soil will be seeded in using the method for the invention processed Picea Mongolica seed, and use fine sand
Covering, its seedling-growing time concentrate on the 8th~14 day after planting, and emergence rate is up to more than 90%, height of seedling 2.3cm or so, variation lines
Number 3.8%.The germination seed that the method for the invention obtains preferably has more than 50% to show money or valuables one carries unintentionally.
With reference to embodiment to a kind of side that Picea Mongolica seed germination rate is improved using gibberellin provided by the invention
Method is described in detail, but they cannot be interpreted as limiting the scope of the present invention.
Embodiment 1
Experimental group and control group are set
Experimental group processing is as follows：
(1) purification of seeds：Using water float glass process, spare Picea Mongolica seed is ducked in drink 20min, removing is kept afloat
On empty not plump seed, pull full seed out.
(2) seed disinfection：Seed in (1) is soaked in 0.5% KMnO4Pull out and drain away the water after solution 2h.
(3) gibberellin is soaked seed：By processed seed in (2) using 0.5mg/L Gibberellins solution seed soaking 24h after pull out
Drain away the water.
(4) presprouting of seeds：Using sand storage method.River sand is eluted with water first and uses 0.5% carbendazim to sterilize, is sprayed after drying
Enter the Gibberellins solution of 0.5mg/L, humidity is advisable to hold agglomerating, loose dissipate, the Picea Mongolica seed that then will be handled well
With river sand with volume ratio 1：It is fitted into after 3 ratio mixing in woven bag and is stacked under the conditions of 10 DEG C, pregermination procedure interval 26h is turned over
Move once, 10 days Picea Mongolica seeds more than 50% show money or valuables one carries unintentionally and can sow.The emergence rate for after planting calculating seed is 91%.
Control group replaces Gibberellins solution immersion Picea Mongolica seed with clear water, soaks river instead of Gibberellins solution with clear water
Sand, other steps are identical with experimental group, and the emergence rate of control group is 64%.
The emergence rate of experimental group seed improves 42% than control group.
Embodiment 2
Experimental group and control group are set
Experimental group processing is as follows：
(3) purification of seeds：Using water float glass process, spare Picea Mongolica seed is ducked in drink 18min, removing is kept afloat
On empty not plump seed, pull full seed out.
(4) seed disinfection：Seed in (1) is soaked in 0.5% KMnO4Pull out and drain away the water after solution 2h.
(3) gibberellin is soaked seed：By processed seed in (2) using 0.5mg/L Gibberellins solution seed soaking 24h after pull out
Drain away the water.
(4) presprouting of seeds：Using sand storage method.River sand is eluted with water first and uses 0.5% carbendazim to sterilize, is sprayed after drying
Enter the Gibberellins solution of 0.5mg/L, humidity is advisable to hold agglomerating, loose dissipate, the Picea Mongolica seed that then will be handled well
With river sand with volume ratio 1：It is fitted into after 3 ratio mixing in woven bag and is stacked under the conditions of 15 DEG C, pregermination procedure interval 24h is turned over
Move once, 7 days Picea Mongolica seeds more than 50% show money or valuables one carries unintentionally and can sow.The emergence rate for after planting calculating seed is 90%.
Control group replaces Gibberellins solution immersion Picea Mongolica seed with clear water, soaks river instead of Gibberellins solution with clear water
Sand, other steps are identical with experimental group, and the emergence rate of control group is 46%.
Experimental group seeding ratio improves 44% than control group.
Embodiment 3
Experimental group and control group are set
Experimental group processing is as follows：
(1) purification of seeds：Using water float glass process, spare Picea Mongolica seed is ducked in drink 20min, removing is kept afloat
On empty not plump seed, pull full seed out.
(2) seed disinfection：Seed in (1) is soaked in 0.5% KMnO4Pull out and drain away the water after solution 2h.
(3) gibberellin is soaked seed：By processed seed in (2) using 0.5mg/L Gibberellins solution seed soaking 24h after pull out
Drain away the water.
(4) presprouting of seeds：Using sand storage method.River sand is eluted with water first and uses 0.5% carbendazim to sterilize, is sprayed after drying
Enter the Gibberellins solution of 0.5mg/L, humidity is advisable to hold agglomerating, loose dissipate, the Picea Mongolica seed that then will be handled well
With river sand with volume ratio 1：It is fitted into after 3 ratio mixing in woven bag and is stacked under the conditions of 12 DEG C, pregermination procedure interval 25h is turned over
Move once, 8 days Picea Mongolica seeds more than 50% show money or valuables one carries unintentionally and can sow.The emergence rate for after planting calculating seed is 94%.
Control group replaces Gibberellins solution immersion Picea Mongolica seed with clear water, soaks river instead of Gibberellins solution with clear water
Sand, other steps are identical with experimental group, and the emergence rate of control group is 72%.
Experimental group seeding ratio improves 30% than control group.
Embodiment 4
(1) purification of seeds：Using water float glass process, spare Picea Mongolica seed is ducked in drink 20min, removing is kept afloat
On empty not plump seed, pull full seed out.
(2) seed disinfection：Seed in (1) is soaked in 0.5% KMnO4Pull out and drain away the water after solution 2h.
(3) gibberellin is soaked seed：By processed seed in (2) using 0.5mg/L Gibberellins solution seed soaking 24h after pull out
Drain away the water.
(4) presprouting of seeds：Using sand storage method.River sand is eluted with water first and uses 0.5% carbendazim to sterilize, is sprayed after drying
Enter clear water, humidity is advisable to hold agglomerating, loose dissipate, then by the Picea Mongolica seed handled well and river sand with volume ratio 1：
It is fitted into after 3 ratio mixing in woven bag and is stacked under the conditions of 15 DEG C, pregermination procedure interval 24h is stirred once, 8 days sand ground clouds
China fir seed more than 50%, which shows money or valuables one carries unintentionally, to be sowed.The emergence rate for after planting calculating seed is 75%.
Embodiment 5
(1) purification of seeds：Using water float glass process, spare Picea Mongolica seed is ducked in drink 20min, removing is kept afloat
On empty not plump seed, pull full seed out.
(2) seed disinfection：Seed in (1) is soaked in 0.5% KMnO4Pull out and drain away the water after solution 2h.
(3) gibberellin is soaked seed：By processed seed in (2) using 0.5mg/L Gibberellins solution seed soaking 24h after pull out
Drain away the water.
(4) presprouting of seeds：Using sand storage method.River sand is eluted with water first and uses 0.5% carbendazim to sterilize, is sprayed after drying
Enter clear water, humidity is advisable to hold agglomerating, loose dissipate, then by the Picea Mongolica seed handled well and river sand with volume ratio 1：
It is fitted into after 3 ratio mixing in woven bag and is stacked under the conditions of 12 DEG C, pregermination procedure interval 25h is stirred once, 9 days sand ground clouds
China fir seed more than 50%, which shows money or valuables one carries unintentionally, to be sowed.The emergence rate for after planting calculating seed is 59%.
Embodiment 6
(1) purification of seeds：Using water float glass process, spare Picea Mongolica seed is ducked in drink 20min, removing is kept afloat
On empty not plump seed, pull full seed out.
(2) seed disinfection：Seed in (1) is soaked in 0.5% KMnO4Pull out and drain away the water after solution 2h.
(3) gibberellin is soaked seed：By processed seed in (2) using 0.5mg/L Gibberellins solution seed soaking 22h after pull out
Drain away the water.
(4) presprouting of seeds：Using sand storage method.River sand is eluted with water first and uses 0.5% carbendazim to sterilize, is sprayed after drying
Enter clear water, humidity is advisable to hold agglomerating, loose dissipate, then by the Picea Mongolica seed handled well and river sand with volume ratio 1：
It is fitted into after 3 ratio mixing in woven bag and is stacked under the conditions of 10 DEG C, pregermination procedure interval 26h is stirred once, 10 days sand grounds
Dragon spruce seed more than 50%, which shows money or valuables one carries unintentionally, to be sowed.The emergence rate for after planting calculating seed is 83%.
From above-described embodiment, the method for the invention can promote seed to sprout, and can effectively break seed dormancy, carry
The emergence rate of high seed is up to more than 90%, and sprout time is more consistent, and emergence is neat.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of method that Picea Mongolica seed germination rate is improved using gibberellin, is comprised the following steps：
1) gibberellin aqueous solution soaking 20~28h of sterile Picea Mongolica seed of 0.3~0.8mg/L is used, obtains pretreatment kind
Son；
2) by step 1) the pretreatment seed with pre-processing river sand using volume ratio as 1：The ratio mixing of (2~5) obtains vernalization
System；
3) vernalization system described in step 2) is stacked into vernalization in 7~10 days in 10~15 DEG C and obtains the seed that shows money or valuables one carries unintentionally.
2. according to the method described in claim 1, it is characterized in that, the concentration of gibberellin aqueous solution described in step 1) is 0.4
~0.6mg/L.
3. according to the method described in claim 1, it is characterized in that, the time soaked seed described in step 1) is 22~26h.
4. according to the method described in claim 1, it is characterized in that, step 2) it is described pretreatment river sand for successively by washing,
Carbendazim disinfection, dry the river sand soaked with Gibberellins solution.
5. the method according to claim 1 or 4, it is characterised in that volume of the pretreatment seed with pre-processing river sand
Than for 1：(3~4).
6. according to the method described in claim 4, it is characterized in that, the mass concentration of the carbendazim is 0.45~0.55%.
7. according to the method described in claim 4, it is characterized in that, soak the concentration of river sand Gibberellins solution for 0.4~
0.6mg/L。
8. according to the method described in claim 1, it is characterized in that, sterile Picea Mongolica seed is by sand ground described in step 1)
Dragon spruce seed is through KMnO4Solution soaks 1~3h and obtains.
9. the according to the method described in claim 8, it is characterized in that, KMnO4The mass concentration of solution is 0.4~0.6%.
10. according to the method described in claim 1, it is characterized in that, to vernalization system during vernalization described in step 2)
Stirred, the time interval stirred is 20~28h.
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| CN201711297858.9A CN108029278B (en) | 2017-12-08 | 2017-12-08 | Method for improving germination rate of picea rasmae seeds by using gibberellin |
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| CN201711297858.9A CN108029278B (en) | 2017-12-08 | 2017-12-08 | Method for improving germination rate of picea rasmae seeds by using gibberellin |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108934272A (en) * | 2018-07-31 | 2018-12-07 | 广西壮族自治区林业科学研究院 | A method of promote Jiangnan keteleeria fortunei seed to sprout |
| CN109005744A (en) * | 2018-09-15 | 2018-12-18 | 烟台市林业科学研究所 | A method of Callicarpa bodinieri Levl. seed germination rate is improved using gibberellin |
| CN109005738A (en) * | 2018-08-16 | 2018-12-18 | 贵州大学 | A method of promoting Chimonocalamus utilis seed germination rate |
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| JPH0856423A (en) * | 1994-08-25 | 1996-03-05 | Sumitomo Chem Co Ltd | Method for improving germination of solanaceous seed |
| CN106171798A (en) * | 2016-08-26 | 2016-12-07 | 甘肃省治沙研究所 | A kind of Picea Mongolica land for growing field crops, Arid Desert Area seed seedling-raising method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JPH0856423A (en) * | 1994-08-25 | 1996-03-05 | Sumitomo Chem Co Ltd | Method for improving germination of solanaceous seed |
| CN106171798A (en) * | 2016-08-26 | 2016-12-07 | 甘肃省治沙研究所 | A kind of Picea Mongolica land for growing field crops, Arid Desert Area seed seedling-raising method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108934272A (en) * | 2018-07-31 | 2018-12-07 | 广西壮族自治区林业科学研究院 | A method of promote Jiangnan keteleeria fortunei seed to sprout |
| CN108934272B (en) * | 2018-07-31 | 2021-01-29 | 广西壮族自治区林业科学研究院 | Method for promoting germination of seeds of sequoia jiangnanensis |
| CN109005738A (en) * | 2018-08-16 | 2018-12-18 | 贵州大学 | A method of promoting Chimonocalamus utilis seed germination rate |
| CN109005744A (en) * | 2018-09-15 | 2018-12-18 | 烟台市林业科学研究所 | A method of Callicarpa bodinieri Levl. seed germination rate is improved using gibberellin |
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| CN108029278B (en) | 2020-12-25 |
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