CN104285542A - Method for promoting germination of tilia miqueliana seeds by magnetic treatment - Google Patents
Method for promoting germination of tilia miqueliana seeds by magnetic treatment Download PDFInfo
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- CN104285542A CN104285542A CN201410611943.8A CN201410611943A CN104285542A CN 104285542 A CN104285542 A CN 104285542A CN 201410611943 A CN201410611943 A CN 201410611943A CN 104285542 A CN104285542 A CN 104285542A
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- 238000000034 method Methods 0.000 title claims abstract description 36
- 230000035784 germination Effects 0.000 title claims abstract description 24
- 241000328973 Tilia miqueliana Species 0.000 title abstract description 17
- 230000001737 promoting effect Effects 0.000 title abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 22
- 238000005530 etching Methods 0.000 claims abstract description 20
- 230000005059 dormancy Effects 0.000 claims abstract description 18
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims abstract description 17
- 239000002253 acid Substances 0.000 claims abstract description 16
- 238000002791 soaking Methods 0.000 claims abstract description 15
- 229930191978 Gibberellin Natural products 0.000 claims abstract description 14
- 239000003448 gibberellin Substances 0.000 claims abstract description 14
- 238000003475 lamination Methods 0.000 claims abstract description 11
- 239000003651 drinking water Substances 0.000 claims description 19
- 235000020188 drinking water Nutrition 0.000 claims description 19
- 240000007313 Tilia cordata Species 0.000 claims description 13
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical class C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 claims description 11
- 238000013517 stratification Methods 0.000 claims description 10
- 238000003756 stirring Methods 0.000 claims description 7
- 239000011521 glass Substances 0.000 claims description 6
- 238000005286 illumination Methods 0.000 claims description 6
- 239000004576 sand Substances 0.000 claims description 6
- 230000007226 seed germination Effects 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 3
- 238000002054 transplantation Methods 0.000 claims description 3
- 238000003672 processing method Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 4
- 238000011161 development Methods 0.000 abstract description 3
- 230000018109 developmental process Effects 0.000 abstract description 3
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 abstract description 3
- 230000004071 biological effect Effects 0.000 abstract 1
- 238000011160 research Methods 0.000 description 4
- 241000219071 Malvaceae Species 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 244000292839 Ficus religiosa Species 0.000 description 1
- 241000907897 Tilia Species 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000005058 diapause Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 230000014284 seed dormancy process Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
Landscapes
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention provides a method for promoting germination of tilia miqueliana seeds by magnetic treatment. Conditions such as the concentrated sulfuric acid etching time of the tilia miqueliana seeds, the seed soaking time of a gibberellin solution and the magnetized water treatment intensity and the frequency in a low-temperature lamination process are strictly controlled; the method is implemented by seed hulling, acid etching by concentrated sulfuric acid, seed soaking by the gibberellin solution, magnetized water treatment, low-temperature lamination and germination environment control; the control on key steps in the dormancy relieving and germination processes of the tilia miqueliana seeds is enhanced, and a magnetic biological effect is applied to the dormancy relieving and germination processes of the seeds, so that the dormancy of the seeds can be quickly relieved, and the seeds are germinated; the effects of high germination speed, tidy germination, high seedling rate and the like are achieved; the technical difficulty in multiplication of the tilia miqueliana seeds is solved; the germination rate of full seeds is over 90 percent, and the seedling rate is over 85 percent; a better sprout foundation is laid for realizing the industrial development of tilia miqueliana seedling cultivation.
Description
Technical field
The present invention relates to achievements in forest-tree seedling breeding field, be specifically related to a kind of magnetic treatment and promote the method that Nanjing linden seeds germinates.
Background technology
Tilia miqueliana (Tilia miqueliana Maxim.) has another name called close gram of linden, white passwood, is commonly called as " bodhi tree ", is Tiliaceae (Tiliaceae) Tilia (Tilia L.) seeds, China's endemic tree.The tilia miqueliana fragrance of a flower is strong fragrant, the large shade of leaf is dense, trunk is tall and straight, integrates afforestation, medicinal, material with, nectariferous plant, because of the religion culture color of its mystery, once by a large amount of introducing and planting of Japan.Tilia miqueliana was once East China indigenous tree species and extensively distributing, but owing to breeding difficulty and habitat destruction, its population quantity and individual amount have been decayed gradually, carried out the task of top priority that propagation technique research becomes tilia miqueliana protection of resources, development and utilization!
Tilia miqueliana propagation technique is based on seminal propagation, but Nanjing linden seeds plumpness is low and deep dormancy characteristic has a strong impact on seedling propagation, becomes the bottleneck problem suppressing tilia miqueliana seeding propagation.At present, be also applied to the report of nursery stock production both at home and abroad without Nanjing linden seeds propagation technique, its main cause is that Nanjing linden seeds plumpness is low, seed breaking dormancy difficulty, seed germination planting percent are low, often causes nursery failure.
Nanjing Forestry University started from 2003, carried out systematicness, continuation scientific research to contents such as tilia miqueliana germ plasm resource, propagation techniques, and study emphasis propagation technique is developed, and has successively carried out the repeatedly research of tilia miqueliana seed propagation technology.Scientific research for many years confirms that tilia miqueliana seedling propagation is very difficult, obtains high quality seedling and to realize Industrialization of seeds and seedlings very difficult.
Through repeatedly attempting, and improve experimental technique gradually, Nanjing linden seeds propagation technique makes a breakthrough, and succeeds.Magnetized water treatment promotes the method that Nanjing linden seeds germinates, comprise and seed is selected, acid etching seed, Seeds Treated with Magnetic Field, hormone is soaked seed, Stratificated treatment, the control that the conditions such as seed sprouting are strict, by wet concentration full seed, concentrated sulfuric acid acid etching seed, magnetized water treatment seed, Gibberellins solution is soaked seed, cold stratification, the management process such as germination that heat up realize, strengthen the control to key link in Nanjing linden seeds termination of diapause and germination process, wet concentration seed improves seed quality, acid etching seed is conducive to increasing seed coat permeability and reducing mechanical obstacles, gibberellin seed soaking is conducive to breaking seed dormancy, magnetic treatment is conducive to seed breaking dormancy and promotes to sprout, integrated treatment optimizes seed breaking dormancy, promote the environment sprouted, ensure that seed can be quick, neat sprouting, good seedling basis has been established for realizing the industrialized development of tilia miqueliana cultivating seedlings.
Summary of the invention
The invention provides a kind of magnetic treatment and promote the method that Nanjing linden seeds germinates, strict control has been carried out to wet concentration seed, acid etching seed, Seeds Treated with Magnetic Field, hormone seed soaking, cool stratification and intensification germination condition, has achieved splendid effect.
The present invention realizes by following technical solution:
Magnetic treatment promotes the method that Nanjing linden seeds germinates, and concrete steps comprise:
(1) wet concentration full seed: use hawkbill to be caught broken shell, taken out by seed, note not clipping broken seed coat, some obvious empty grains or damaged seed should be rejected in time.The seed that will shell puts into washbowl or bulk container, and glass bar fully stirs; After 30min, removed by the seed floating on the water surface, be sunken to bottom seed and take out and drain moisture, this part seed is full seed; Full seed is placed on 3 ~ 5 layers of newspaper or blotting paper, and the surface of the seed moisture blots by paper using.
(2) concentrated sulfuric acid acid etching seed: 100 ~ 150g seed is placed in 1000ml beaker, adds the concentrated sulfuric acid of 250 ~ 300ml; Concentrated sulfuric acid etching time is 40 ~ 50min, period, and glass bar continues slowly to stir; Slowly inclined to by the concentrated sulfuric acid in beaker, the seed after acid etching uses running water fully to clean, and running water 24h.
(3) magnetized water treatment seed: in seed breaking dormancy and germination process, all uses magnetized water treatment seed.
Affiliated magnetized water treatment seed is: 1) Gibberellins solution uses the preparation of 10000 ~ 15000GS magnetized drinking water; 2) in lamination process, moisture is provided by 10000 ~ 15000GS magnetized drinking water; 3) in germination process, moisture is provided by 10000 ~ 15000GS magnetized drinking water.
(4) Gibberellins solution seed soaking: use the seed 48 ~ 60h after the Gibberellins solution seed soaking acid etching of 1000 ~ 1500mg/L;
(5) cold stratification: the seed after seed soaking is placed in wet rough sand, and planting husky volume ratio is 1: 3, puts into 0 ~ 5 DEG C of environment and carries out cold stratification; Rough sand adds magnetized drinking water, and addition is advisable with " hold agglomerating, unclamp and namely fall apart ".In lamination process, stirred seed once every 5 days, and according to circumstances suitably add magnetized drinking water.Lamination is after 50 ~ 60 days, and seed and breaking dormancy enter germinating.
(6) heat up germination: be transferred in 23 ± 2 DEG C of illumination boxs by the seed entering germinating by low temperature environment, intensity of illumination 10000 ~ 15000LX, light application time 10 ~ 12h/ days, sprouting situation is observed the next day of duration of germination, add magnetized drinking water in time, until after cotyledon launches completely, carry out bud transplantation of seedlings.
With this processing method, seed germination rate can reach the level of 90%, transplants planting percent more than 85%.
The advantage of technical solution of the present invention is:
(1) wet concentration full seed improves seed quality, by empty grain and grow imperfect seed reject, decrease seed subsequent treatment quantity, improve seed germination rate, meanwhile, reduce the probability rotted in earthing of seeds process.
(2) concentrated sulfuric acid acid etching seed, increases seed coat permeability, reduces the mechanical obstacles of seed coat, is conducive to seed breaking dormancy and sprouts, and meanwhile, concentrated sulfuric acid acid etching can also play the effect of sterilization to seed coat surface.
(3) Gibberellins solution seed soaking, improves Interior Seed GA/ABA ratio, can indirectly shorten the cool stratification time, be conducive to seed breaking dormancy and sprout.
(4) cool stratification, can promote that seed removes physiological dormancy, promote seed germination, improve seed germination regularity.
(5) Seeds Treated with Magnetic Field, multiple links of seed treatment use magnetized drinking water, make all to use magnetic treatment in seed breaking dormancy and germination process, magnetic treatment is conducive to neutron absorption moisture, increases respiratory intensity, promotes Interior Seed physiological activator to increase and improve related enzyme activity, promotes seed breaking dormancy and sprouting.
Embodiment
Now adopt following embodiment to be further described the present invention, but the scope involved by invention is not restricted to following examples.
Embodiment 1
Magnetic treatment promotes the method that Nanjing linden seeds germinates, and concrete steps comprise:
1, seed shells
Use hawkbill to be caught broken shell, taken out by seed, note not clipping broken seed coat, some obvious empty grains or damaged seed should be rejected in time.
2, wet concentration full seed
The seed that will shell puts into washbowl or bulk container, and glass bar fully stirs; After 30min, removed by the seed floating on the water surface, be sunken to bottom seed and take out and drain moisture, this part seed is full seed; Full seed is placed on 3 ~ 5 layers of newspaper or blotting paper, and the surface of the seed moisture blots by paper using.
3, concentrated sulfuric acid acid etching seed
100 ~ 150g seed is placed in 1000ml beaker, adds the concentrated sulfuric acid of 250 ~ 300ml; Concentrated sulfuric acid etching time is 40 ~ 50min, period, and glass bar continues slowly to stir; Slowly inclined to by the concentrated sulfuric acid in beaker, the seed after acid etching uses running water fully to clean, and running water 24h.
4, magnetized water treatment seed
In seed breaking dormancy and germination process, all use magnetized water treatment seed.Magnetized water treatment seed is divided into three links: Gibberellins solution uses the preparation of 10000 ~ 15000GS magnetized drinking water; In lamination process, moisture is provided by 10000 ~ 15000GS magnetized drinking water; In germination process, moisture is provided by 10000 ~ 15000GS magnetized drinking water.
5, Gibberellins solution seed soaking
Use the seed 48 ~ 60h after the Gibberellins solution seed soaking acid etching of 1000 ~ 1500mg/L, frequently stir in seed soaking process, make the abundant absorbent solution of seed.
6, cold stratification
Seed after seed soaking is placed in wet rough sand, and planting husky volume ratio is 1: 3, puts into 0 ~ 5 DEG C of environment and carries out cold stratification; Rough sand adds magnetized drinking water, and addition is advisable with " hold agglomerating, unclamp and namely fall apart ".In lamination process, stirred seed once every 5 days, and according to circumstances suitably add magnetized drinking water.Lamination is after 50 ~ 60 days, and seed and breaking dormancy enter germinating.
7, heat up germination
The seed entering germinating is transferred in 23 ± 2 DEG C of illumination boxs by low temperature environment, intensity of illumination 10000 ~ 15000LX, light application time 10 ~ 12h/ days, sprouting situation is observed the next day of duration of germination, add magnetized drinking water in time, until after cotyledon launches completely, carry out bud transplantation of seedlings.
Claims (1)
1. magnetic treatment promotes to it is characterized in that the method that Nanjing linden seeds germinates, and adopts following steps:
(1) wet concentration full seed: use hawkbill to be caught broken shell, taken out by seed, note not clipping broken seed coat, some obvious empty grains or damaged seed should be rejected in time.The seed that will shell puts into washbowl or bulk container, and glass bar fully stirs; After 30min, removed by the seed floating on the water surface, be sunken to bottom seed and take out and drain moisture, this part seed is full seed; Full seed is placed on 3 ~ 5 layers of newspaper or blotting paper, and the surface of the seed moisture blots by paper using.
(2) concentrated sulfuric acid acid etching seed: 100 ~ 150g seed is placed in 1000ml beaker, adds the concentrated sulfuric acid of 250 ~ 300ml; Concentrated sulfuric acid etching time is 40 ~ 50min, period, and glass bar continues slowly to stir; Slowly inclined to by the concentrated sulfuric acid in beaker, the seed after acid etching uses running water fully to clean, and running water 24h.
(3) magnetized water treatment seed: in seed breaking dormancy and germination process, all uses magnetized water treatment seed.
Affiliated magnetized water treatment seed is: 1) Gibberellins solution uses the preparation of 10000 ~ 15000GS magnetized drinking water; 2) in lamination process, moisture is provided by 10000 ~ 15000GS magnetized drinking water; 3) in germination process, moisture is provided by 10000 ~ 15000GS magnetized drinking water.
(4) Gibberellins solution seed soaking: use the seed 48 ~ 60h after the Gibberellins solution seed soaking acid etching of 1000 ~ 1500mg/L;
(5) cold stratification: the seed after seed soaking is placed in wet rough sand, and planting husky volume ratio is 1: 3, puts into 0 ~ 5 DEG C of environment and carries out cold stratification; Rough sand adds magnetized drinking water, and addition is advisable with " hold agglomerating, unclamp and namely fall apart ".In lamination process, stirred seed once every 5 days, and according to circumstances suitably add magnetized drinking water.Lamination is after 50 ~ 60 days, and seed and breaking dormancy enter germinating.
(6) heat up germination: be transferred in 23 ± 2 DEG C of illumination boxs by the seed entering germinating by low temperature environment, intensity of illumination 10000 ~ 15000LX, light application time 10 ~ 12h/ days, sprouting situation is observed the next day of duration of germination, add magnetized drinking water in time, until after cotyledon launches completely, carry out bud transplantation of seedlings.
With this processing method, seed germination rate can reach the level of 90%, transplants planting percent more than 85%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201410611943.8A CN104285542B (en) | 2014-10-30 | 2014-10-30 | A kind of magnetic treatment promotes the method that Nanjing linden seeds germinates |
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| CN201410611943.8A CN104285542B (en) | 2014-10-30 | 2014-10-30 | A kind of magnetic treatment promotes the method that Nanjing linden seeds germinates |
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| CN104285542B CN104285542B (en) | 2016-05-25 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105284233A (en) * | 2015-02-04 | 2016-02-03 | 王振月 | Germination accelerating method of northeastern crowberry seeds |
| CN108834473A (en) * | 2018-07-18 | 2018-11-20 | 南京林业大学 | A method of releasing seed dormancy |
| CN110583146A (en) * | 2019-10-14 | 2019-12-20 | 安徽格瑞恩园林工程有限公司 | Treatment method for improving germination rate of Tilia miqueliana seeds |
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| CN101120634A (en) * | 2007-09-11 | 2008-02-13 | 南京林业大学 | Method for rapid relieving dormant state of nanjing linden seed |
| CN102599026A (en) * | 2012-03-30 | 2012-07-25 | 常熟市常福有机复合肥有限公司 | Tilia miqueliana seedling raising method |
| CN102668851A (en) * | 2012-05-29 | 2012-09-19 | 承德市神洲药业开发有限公司 | Linden cultivation method |
| RU2492625C2 (en) * | 2011-10-27 | 2013-09-20 | Государственное научное учреждение Поволжский научно-исследовательский институт производства и переработки мясомолочной продукции Российской академии сельскохозяйственных наук | Method of stimulation of seeds germination of agricultural crops |
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2014
- 2014-10-30 CN CN201410611943.8A patent/CN104285542B/en not_active Expired - Fee Related
Patent Citations (4)
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| CN101120634A (en) * | 2007-09-11 | 2008-02-13 | 南京林业大学 | Method for rapid relieving dormant state of nanjing linden seed |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105284233A (en) * | 2015-02-04 | 2016-02-03 | 王振月 | Germination accelerating method of northeastern crowberry seeds |
| CN105284233B (en) * | 2015-02-04 | 2017-12-29 | 王振月 | A kind of Northeastern crowberry seed accelerating germination method |
| CN108834473A (en) * | 2018-07-18 | 2018-11-20 | 南京林业大学 | A method of releasing seed dormancy |
| CN110583146A (en) * | 2019-10-14 | 2019-12-20 | 安徽格瑞恩园林工程有限公司 | Treatment method for improving germination rate of Tilia miqueliana seeds |
| CN110583146B (en) * | 2019-10-14 | 2024-01-19 | 安徽格瑞恩园林工程有限公司 | Treatment method for improving germination rate of Tilia miqueliana seeds |
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Application publication date: 20150121 Assignee: Yangzhou little apple gardening Co., Ltd. Assignor: Nanjing Forestry University Contract record no.: 2018320000233 Denomination of invention: Method for promoting germination of tilia miqueliana seeds by magnetic treatment Granted publication date: 20160525 License type: Common License Record date: 20181024 Application publication date: 20150121 Assignee: Beijing Huamei Wanxiang Technology Co., Ltd. Assignor: Nanjing Forestry University Contract record no.: 2018320000235 Denomination of invention: Method for promoting germination of tilia miqueliana seeds by magnetic treatment Granted publication date: 20160525 License type: Common License Record date: 20181024 |
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