CN107836710B - High-absorptivity iron-supplementing peptide-rich food base material and preparation method and application thereof - Google Patents

High-absorptivity iron-supplementing peptide-rich food base material and preparation method and application thereof Download PDF

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CN107836710B
CN107836710B CN201711172584.0A CN201711172584A CN107836710B CN 107836710 B CN107836710 B CN 107836710B CN 201711172584 A CN201711172584 A CN 201711172584A CN 107836710 B CN107836710 B CN 107836710B
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peptide
iron
supplementing
absorptivity
mass
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CN107836710A (en
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张业辉
张友胜
汪婧瑜
阮奇珺
刘学铭
陈智毅
王旭苹
程镜蓉
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Sericulture and Agri Food Research Institute GAAS
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Abstract

The invention belongs to the field of agricultural product processing and application, and discloses a high-absorptivity iron-supplementing peptide-rich food base material, and a preparation method and application thereof. The method takes chicken breast meat paste as a raw material, and adds trypsin and alkaline protease for hydrolysis to obtain chicken peptide; uniformly mixing stevioside and chicken peptide, carrying out ultrasonic intermittent treatment, and adding ferrous fumarate during the ultrasonic treatment to obtain a mixed solution; pulping the soda ash, filtering to obtain Sucus Perillae Frutescentis juice, adding into the mixed solution, stirring with a homogenizer, and homogenizing with temperature-controlled micro-jet; adding sorbitol and whey protein powder into the homogenized mixed solution, and adding lactobacillus; performing pulse sterilization to obtain a fermented peptide-rich raw material; spray drying the fermented peptide-rich raw material to obtain dry powder, namely the high-absorptivity iron-supplementing peptide-rich food base material; and (7) sealing and packaging. The food base material can be prepared into high-absorptivity iron-supplementing peptide meal replacement powder. The iron absorption of human body is enhanced through normal diet without adverse side effect.

Description

High-absorptivity iron-supplementing peptide-rich food base material and preparation method and application thereof
Technical Field
The invention belongs to the field of agricultural product processing and application, and particularly relates to a high-absorptivity iron-supplementing peptide-rich food base material and a preparation method and application thereof.
Background
Iron-deficiency anemia people widely exist in China, the demand and supply of organisms to iron are unbalanced, and the incidence rate of the iron-deficiency anemia is obviously increased in developing countries, regions with underdeveloped economy, infants, women in childbearing age and other people. The survey in 2010 in Shanghai area of China shows that the annual incidence of iron deficiency is 75.0-82.5% of infants and children between 6 months and 2 years old, 66.7% of women over 3 months of gestation, 43.3% of women of childbearing age and 13.2% of teenagers between 10 years and 17 years old. In addition to certain causes in the body leading to impaired iron absorption and excessive iron loss, increased iron demand and insufficient iron intake are important causes thereof. For example, pregnant and nursing women have a large demand for iron, and are also prone to iron-deficiency anemia, thereby reducing body immunity and affecting health.
Some traditional food materials with the iron supplementing effect, such as donkey-hide gelatin, black fungus, spinach, red dates, pig liver and the like, also have a certain iron supplementing effect, but the effect is slow, and the food materials also need to be mixed with vitamins and the like for taking to have better effect, and need to be eaten for a long time or in large quantities, so that the appetite is influenced, and the absorption and utilization of other nutrients are easily interfered. At present, iron supplement medicines, foods and nutritional agents sold in the market are expensive, and the iron absorption is promoted mostly by increasing the iron content in the medicines, but after a large amount of organic acid iron salt is taken into a human body, the normal nutrient intake of the body is interfered, and the like. This patent uses chicken peptide and stevioside to carry out the complex formulation as the base material, utilizes the flavonoid composition in the shan su to promote peptide iron stable in structure, characteristics such as biological fermentation increase of utilization ratio produce easily by human intestinal absorption's iron supplementation peptide food base material.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention mainly aims to provide a preparation method of a high-absorptivity iron-supplementing peptide-rich food base material. The iron supplement food prepared by the method utilizes natural raw material glycoside peptide to carry out covalent bonding with iron ions to form a stable peptide iron structure, and then forms a natural targeting release effect of small intestine under the action of Sucus; by utilizing the characteristic that the small peptide of biological fermentation is easily absorbed by the intestinal tract of a human body and forming the peptide iron with a stable structure, the flavor of the iron-supplementing food base material can be enhanced, and the effect of supplementing iron can be achieved.
The invention also aims to provide the high-absorptivity iron-supplementing peptide-rich food base material prepared by the preparation method.
The invention further aims to provide application of the high-absorptivity iron-supplementing peptide-rich food base material.
The purpose of the invention is realized by the following scheme:
a preparation method of a high-absorptivity iron-supplementing peptide-rich food base material comprises the following operation steps:
(1) taking chicken breast meat paste crushed by a wet method as a raw material, and adding 20000u/g trypsin and 8000u/g alkaline protease for hydrolysis, wherein the hydrolysis time is 3-5 hours, so that the chicken breast meat paste is hydrolyzed into chicken peptide with the molecular weight not more than 800 Da;
(2) uniformly mixing stevioside with chicken peptide according to the weight ratio of 1:3-1:5, carrying out intermittent treatment for 26-38 minutes by adopting 300-500W ultrasonic waves, keeping the temperature at 38-42 ℃ all the time, and adding ferrous fumarate during the ultrasonic wave treatment to obtain a mixed solution; the addition amount of the ferrous fumarate is 100-140mg per 100g of the mixture of the stevioside and the chicken peptide;
(3) crushing plant common perilla, grinding into slurry, vacuum filtering with a 200-mesh screen, adding the filtered common perilla juice into the mixed solution in the step (2) according to the amount of 0.8-1.0 wt%, stirring for 20-30 minutes by a refiner, and homogenizing for 1 time by temperature-controlled micro-jet;
(4) adding 0.05-0.15 wt% of sorbitol and 2-3 wt% of whey protein powder into the homogenized mixed solution obtained in the step (3), adding lactobacillus, controlling the temperature at 33-37 ℃, fermenting for 3-5 hours until the thallus concentration reaches 103～104CFU/mL; performing pulse sterilization to obtain a fermented peptide-rich raw material;
(5) spray drying the fermented peptide-rich raw material to obtain dry powder, namely the iron-supplementing peptide-rich food base material with high absorptivity; and (7) sealing and packaging.
The fineness of the chicken breast meat paste in the step (1) is 80-120 meshes.
The temperature control micro-jet in the step (3) is controlled at the pressure of 100-120MPa and the temperature of 4-10 ℃.
A high-absorptivity iron-supplementing peptide-rich food base material prepared by the preparation method is provided.
The high-absorptivity iron-supplementing peptide-rich food base material is applied to preparation of high-absorptivity iron-supplementing peptide-rich meal replacement powder.
The high-absorptivity iron-supplementing peptide meal replacement powder is prepared by uniformly compounding and mixing 5-7% by mass of a high-absorptivity iron-supplementing peptide food base material, 15-17% by mass of crystalline fructose, 12-16% by mass of maltodextrin, 13-15% by mass of soybean protein isolate, 13-15% by mass of brown rice powder, 7.5-8.5% by mass of whole milk powder, 6-8% by mass of walnut kernels, 3.5-5.5% by mass of fructo-oligosaccharide, 3-4% by mass of medium-chain triglyceride powder, 2.5-3.5% by mass of agaric powder, vitamin C and vitamin E, and sealing and packaging.
The high-absorptivity iron-supplementing peptide meal replacement powder is brewed with warm water to eat according to the mass ratio of 1: 3.
By eating the food base material of the invention, the absorption of iron is enhanced, various human diseases or sub-health states caused by iron deficiency anemia are reduced, and the adverse effects of the existing iron supplement food on human bodies mainly by the technologies of increasing the content of iron ions and the like are changed.
Compared with the prior art, the invention has the following advantages and beneficial effects:
(1) compared with the traditional iron supplement product, the invention utilizes the glycosidic peptide of natural components to carry out covalent combination with iron ions under the action of ultrasonic waves to form a stable peptide-iron structure, and then forms a stable small-particle polymer through the natural micro-embedding effect of the common perilla juice, and is characterized in that the peptide-iron conjugate is directionally targeted and released in the small intestine, and the relatively complete peptide-iron structure can be kept to reach the absorption part of the small intestine; the peptide iron with stable structure can enhance the flavor of the iron-supplementing food base material and achieve the effect of iron supplementation.
(2) Compared with the traditional product for supplementing iron elements, firstly, the chicken with mild property is selected as the raw material, natural raw material components such as stevia rebaudiana and common perilla are used as auxiliary materials, and the nutrition components are reasonably matched; can be used for replacing breakfast and the like for a long time, and can enhance the iron absorption of human body through normal diet without adverse side effect.
(3) The iron-supplementing peptide-rich powder prepared by the fermentation technology has natural and pleasant flavor, does not need to be blended by adding artificial spices, has better quality compared with the traditional iron-supplementing product, and is easier to be accepted by children, old people and other people with sensitive taste.
Detailed Description
The present invention will be described in further detail with reference to examples, but the embodiments of the present invention are not limited thereto.
Example 1
(1) Taking chicken breast meat paste crushed by a wet method as a raw material (80 meshes), adding trypsin (20000u/g) and alkaline protease (8000u/g) for hydrolysis for 4 hours to hydrolyze the chicken breast meat paste into chicken peptide with the molecular weight not more than 800 Da;
(2) uniformly mixing stevioside and chicken peptide according to the weight ratio of 1:5, carrying out intermittent ultrasonic treatment (400W) for 38 minutes, keeping the temperature at 40 ℃ all the time, and adding ferrous fumarate (100 mg of ferrous fumarate is added to each 100g of the mixture of the stevioside and the chicken peptide) in the ultrasonic treatment to obtain a mixed solution;
(3) crushing plant herba cistanches, grinding into slurry, vacuum filtering with a 200-mesh screen, adding the filtered herba cistanches juice into the mixed solution obtained in the step (2) according to the proportion of 1.0 wt%, stirring for 20 minutes by a refiner, and homogenizing for 1 time (120MPa, temperature 7 ℃) by temperature-controlled micro-jet;
(4) adding 0.15 wt% of sorbitol and 2.5 wt% of whey protein powder into the homogenized mixed solution obtained in the step (3), adding lactobacillus, controlling the temperature at 37 ℃, and fermenting for 3 hours until the thallus concentration reaches 104CFU/mL; performing pulse sterilization to obtain a fermented peptide-rich raw material;
(5) spray drying the fermented peptide-rich raw material to obtain dry powder, namely the iron-supplementing peptide-rich food base material with high absorptivity; and (7) sealing and packaging.
(6) And uniformly compounding and mixing the obtained high-absorptivity iron-supplementing peptide-rich food base material with the mass percentage of 5%, crystalline fructose with the mass percentage of 16%, maltodextrin with the mass percentage of 12%, soybean protein isolate with the mass percentage of 14%, brown rice powder with the mass percentage of 13%, whole milk powder with the mass percentage of 8.5%, walnut kernel with the mass percentage of 7%, fructo-oligosaccharide with the mass percentage of 3.5%, medium-chain triglyceride powder with the mass percentage of 4%, agaric powder with the mass percentage of 3.5%, vitamin C and vitamin E, and sealing and packaging to obtain the high-absorptivity iron-supplementing peptide-rich meal replacement powder. The high-absorptivity iron-supplementing peptide meal replacement powder is brewed with warm water according to the mass ratio of 1:3 and is eaten.
Example 2
(1) Taking chicken breast meat paste crushed by a wet method as a raw material (100 meshes), adding trypsin (20000u/g) and alkaline protease (8000u/g) for hydrolysis for 5 hours to hydrolyze the chicken breast meat paste into chicken peptide with the molecular weight not more than 800 Da;
(2) mixing stevioside and chicken peptide at a weight ratio of 1:4, performing ultrasonic treatment (300W) for 32 min at 38 deg.C, and adding ferrous fumarate (120 mg per 100g mixture of stevioside and chicken peptide) during ultrasonic treatment to obtain mixed solution
(3) Crushing plant herba cistanches, grinding into slurry, vacuum filtering with a 200-mesh screen, adding filtered herba cistanches juice into the mixed solution in the step (2) according to the proportion (0.8 wt%), stirring for 20 minutes by a refiner, and homogenizing for 1 time (100MPa, temperature 4 ℃) by temperature-controlled micro-jet;
(4) adding 0.05 wt% of sorbitol and 2 wt% of whey protein powder into the homogenized mixed solution obtained in the step (3), adding lactobacillus, controlling the temperature at 33 ℃, and fermenting for 4 hours until the thallus concentration reaches 103CFU/mL; performing pulse sterilization to obtain a fermented peptide-rich raw material;
(5) spray drying the fermented peptide-rich raw material to obtain dry powder, namely the iron-supplementing peptide-rich food base material with high absorptivity; and (7) sealing and packaging.
(6) The high-absorptivity iron-supplementing peptide-rich food base material with the mass percentage of 6 percent, the crystalline fructose with the mass percentage of 15 percent, the maltodextrin with the mass percentage of 14 percent, the soybean protein isolate with the mass percentage of 13 percent, the brown rice powder with the mass percentage of 14 percent, the whole milk powder with the mass percentage of 7.5 percent, the walnut kernel with the mass percentage of 8 percent, the fructo-oligosaccharide with the mass percentage of 5.5 percent, the medium-chain triglyceride powder with the mass percentage of 3.5 percent, the agaric powder with the mass percentage of 2.5 percent, the vitamin C and the vitamin E are compounded, mixed evenly, sealed and packaged to obtain the high-absorptivity iron-supplementing peptide-rich meal replacement powder. The high-absorptivity iron-supplementing peptide meal replacement powder is brewed with warm water according to the mass ratio of 1:3 and is eaten.
Example 3
(1) Taking chicken breast meat paste crushed by a wet method as a raw material (120 meshes), adding trypsin (20000u/g) and alkaline protease (8000u/g) for hydrolysis for 3 hours to hydrolyze the chicken breast meat paste into chicken peptide with the molecular weight not more than 800 Da;
(2) uniformly mixing stevioside and chicken peptide according to a weight ratio (1:3), carrying out intermittent ultrasonic treatment (500W) for 26 minutes, keeping the temperature at 42 ℃ all the time, and adding ferrous fumarate (100-140 mg of ferrous fumarate is added to each 100g of the mixture of the stevioside and the chicken peptide) during the ultrasonic treatment to obtain a mixed solution;
(3) crushing plant herba cistanches, grinding into slurry, vacuum filtering with a 200-mesh screen, adding filtered herba cistanches juice into the mixed solution in the step (2) according to the proportion (0.9 wt%), stirring for 30 minutes by a refiner, and homogenizing for 1 time (110MPa, temperature 10 ℃) by temperature-controlled micro-jet;
(4) adding 0.10 wt% of sorbitol and 3 wt% of whey protein powder into the homogenized mixed solution obtained in the step (3), adding lactobacillus, controlling the temperature to be 35 ℃, and fermenting for 5 hours until the thallus concentration reaches 104CFU/mL; performing pulse sterilization to obtain a fermented peptide-rich raw material;
(5) spray drying the fermented peptide-rich raw material to obtain dry powder, namely the iron-supplementing peptide-rich food base material with high absorptivity; and (7) sealing and packaging.
(6) And compounding and uniformly mixing the obtained high-absorptivity iron-supplementing peptide-rich food base material with the mass percent of 7%, crystalline fructose with the mass percent of 17%, maltodextrin with the mass percent of 14%, soybean protein isolate with the mass percent of 15%, brown rice powder with the mass percent of 15%, whole milk powder with the mass percent of 8%, walnut kernel with the mass percent of 6%, fructo-oligosaccharide with the mass percent of 4.5%, medium-chain triglyceride powder with the mass percent of 3%, agaric powder with the mass percent of 3%, vitamin C and vitamin E, and sealing and packaging to obtain the high-absorptivity iron-supplementing peptide-rich meal replacement powder. The high-absorptivity iron-supplementing peptide meal replacement powder is brewed with warm water according to the mass ratio of 1:3 and is eaten.
The high-absorptivity iron-supplement peptide-rich food base material obtained in examples 1 to 3, which may also be referred to as iron-supplement peptide-rich powder, was subjected to performance tests, and the results are shown in table 1:
TABLE 1 absorption rate of Caco-2 cells to iron-supplementing peptide-rich powder and its quality characteristics
Figure BDA0001477538850000071
Note: (1) the Caco-2 cell absorption rate detection method comprises the following steps:
research progress in the Caco-2 cell model to predict active substance absorption metabolism, 2015,15 (1): 164-172 (2) flavor component detection method: (GC-MS gas chromatography detection method)
Flavor component detection SPME-GC-MS detection of fast fermented goldfish volatile flavor components according to literature [ J ], food and machinery, 2009, 5 (6): 102-106.
(3) The protein content detection method comprises the following steps:
determination of protein in GB 50095-activated 2010 food safety national standard food
(4)Fe2+The content detection method comprises the following steps:
research on-site rapid detection technology of trace elements such as iron and zinc in functional food, the scientific academy of military medical science of the liberation army of people in China, 2012, master thesis.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.

Claims (7)

1. The preparation method of the high-absorptivity iron-supplementing peptide-rich food base material is characterized by comprising the following operation steps of:
(1) taking chicken breast meat paste crushed by a wet method as a raw material, and adding 20000u/g trypsin and 8000u/g alkaline protease for hydrolysis, wherein the hydrolysis time is 3-5 hours, so that the chicken breast meat paste is hydrolyzed into chicken peptide with the molecular weight not more than 800 Da;
(2) uniformly mixing stevioside with chicken peptide according to the weight ratio of 1:3-1:5, carrying out intermittent treatment for 26-38 minutes by adopting 300-500W ultrasonic waves, keeping the temperature at 38-42 ℃ all the time, and adding ferrous fumarate during the ultrasonic treatment to obtain a mixed solution; the addition amount of the ferrous fumarate is 100-140mg per 100g of the mixture of the stevioside and the chicken peptide;
(3) crushing plant herba cistanches, grinding into slurry, vacuum filtering with a 200-mesh screen, adding filtered herba cistanches juice into the mixed solution in the step (2) according to the amount of 0.8-1.0 wt%, stirring for 20-30 minutes by a refiner, and homogenizing for 1 time by temperature-controlled micro-jet;
(4) adding 0.05-0.15 wt% of sorbitol and 2-3 wt% of whey protein powder into the homogenized mixed solution obtained in the step (3), adding lactobacillus, controlling the temperature to be 33-37 ℃, fermenting for 3-5 hours until the thallus concentration reaches 103～104CFU/mL; performing pulse sterilization to obtain a fermented peptide-rich raw material;
(5) spray drying the fermented peptide-rich raw material to obtain dry powder, namely the high-absorptivity iron-supplementing peptide-rich food base material; and (7) sealing and packaging.
2. The method for preparing the iron-supplementing peptide-rich food base material with high absorptivity according to claim 1, wherein the method comprises the following steps: the fineness of the chicken breast meat paste in the step (1) is 80-120 meshes.
3. The method for preparing the iron-supplementing peptide-rich food base material with high absorptivity according to claim 1, wherein the method comprises the following steps: the temperature control micro-jet in the step (3) is controlled at the pressure of 100-120MPa and the temperature of 4-10 ℃.
4. A high-absorptivity iron-supplementing peptide-rich food base prepared according to the preparation method of claim 1.
5. The use of the high absorption rate iron supplement peptide-rich food base according to claim 4 for preparing a high absorption rate iron supplement peptide-rich meal replacement powder.
6. Use according to claim 5, characterized in that: the high-absorptivity iron-supplementing peptide meal replacement powder is prepared by uniformly compounding and mixing 5-7% by mass of a high-absorptivity iron-supplementing peptide food base material, 15-17% by mass of crystalline fructose, 12-16% by mass of maltodextrin, 13-15% by mass of soybean protein isolate, 13-15% by mass of brown rice powder, 7.5-8.5% by mass of whole milk powder, 6-8% by mass of walnut kernels, 3.5-5.5% by mass of fructo-oligosaccharide, 3-4% by mass of medium-chain triglyceride powder, 2.5-3.5% by mass of agaric powder, vitamin C and vitamin E, and sealing and packaging.
7. Use according to claim 5, characterized in that: the high-absorptivity iron-supplementing peptide meal replacement powder is brewed with warm water to eat according to the mass ratio of 1: 3.
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