CN107836710A - A kind of high-absorbility mends iron richness peptide foodstuff base material and its preparation method and application - Google Patents
A kind of high-absorbility mends iron richness peptide foodstuff base material and its preparation method and application Download PDFInfo
- Publication number
- CN107836710A CN107836710A CN201711172584.0A CN201711172584A CN107836710A CN 107836710 A CN107836710 A CN 107836710A CN 201711172584 A CN201711172584 A CN 201711172584A CN 107836710 A CN107836710 A CN 107836710A
- Authority
- CN
- China
- Prior art keywords
- peptide
- absorbility
- iron
- base material
- mass percent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 title claims abstract description 151
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 80
- 229910052742 iron Inorganic materials 0.000 title claims abstract description 77
- 239000000463 material Substances 0.000 title claims abstract description 34
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 239000000843 powder Substances 0.000 claims abstract description 42
- 239000002994 raw material Substances 0.000 claims abstract description 21
- 241000287828 Gallus gallus Species 0.000 claims abstract description 19
- 235000012054 meals Nutrition 0.000 claims abstract description 13
- 235000002332 ferrous fumarate Nutrition 0.000 claims abstract description 12
- 239000011259 mixed solution Substances 0.000 claims abstract description 12
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- PMVSDNDAUGGCCE-TYYBGVCCSA-L Ferrous fumarate Chemical compound [Fe+2].[O-]C(=O)\C=C\C([O-])=O PMVSDNDAUGGCCE-TYYBGVCCSA-L 0.000 claims abstract description 7
- UEDUENGHJMELGK-HYDKPPNVSA-N Stevioside Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O UEDUENGHJMELGK-HYDKPPNVSA-N 0.000 claims abstract description 7
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- OHHNJQXIOPOJSC-UHFFFAOYSA-N stevioside Natural products CC1(CCCC2(C)C3(C)CCC4(CC3(CCC12C)CC4=C)OC5OC(CO)C(O)C(O)C5OC6OC(CO)C(O)C(O)C6O)C(=O)OC7OC(CO)C(O)C(O)C7O OHHNJQXIOPOJSC-UHFFFAOYSA-N 0.000 claims abstract description 7
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- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims description 4
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- MVORZMQFXBLMHM-QWRGUYRKSA-N Gly-His-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)CN)CC1=CN=CN1 MVORZMQFXBLMHM-QWRGUYRKSA-N 0.000 description 3
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
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Abstract
The invention belongs to processing of farm products and application field, discloses a kind of high-absorbility and mends iron richness peptide foodstuff base material and its preparation method and application.This method is using Fresh Grade Breast meat gruel as raw material, adds trypsase and alkali protease is hydrolyzed to obtain chicken peptide;Stevioside is well mixed with chicken peptide, ultrasonic wave discontinuity treatment, ferrous fumarate is added during ultrasonication, obtains mixed solution;Mountain soda comminution is starched, filtering gained mountain Soviet Union juice is added in mixed solution, refiner stirring, then passes through temperature control microjet homogeneous;D-sorbite and PURE WHEY are added into the mixed liquor after homogeneous, then adds Bacillus acidi lactici;Horizontal pulse of going forward side by side sterilizes, and obtains the rich peptide raw material that ferments;The rich peptide raw material that will ferment is spray-dried, and gained dry powder is that high-absorbility mends iron richness peptide foodstuff base material;Pack.The foodstuff base material can be prepared into high-absorbility and mend iron richness peptide meal replacement powder.Absorption of the human body to iron, no adverse side effect are strengthened by normal diet.
Description
Technical field
The invention belongs to processing of farm products and application field, and in particular to a kind of high-absorbility mends iron richness peptide foodstuff base material
And its preparation method and application.
Background technology
Hypoferric anemia crowd is widely present in China, is that demand and supply of the body to iron is unbalance, its incidence of disease is being sent out
Substantially increase in the crowd such as national, economically less developed region and infant, the women of child-bearing age in exhibition.China District of Shanghai is 2010
Year investigation display, the annual morbidity of asiderosis was in 6 months~2 years old infant 75.0%~82.5%, gestation more than 3 months
Women 66.7%, the women of child-bearing age 43.3%, 10 years old~17 years old teenager 13.2%.Except some causes of disease in body cause iron
Malabsorption and iron are lost excessive outer, need iron to increase and iron insufficiency of intake the reason for being wherein important.Such as period of pregnancy and the food in one's mouth
The women of newborn phase, is in great demand to iron, also easily causes hypoferric anemia, unhealthful so as to reduce immunity of organisms
It is horizontal.
Some traditional food materials with benefit iron effect, such as donkey-hide gelatin, black fungus, spinach, jujube and pork liver etc. also have
It is certain to mend iron effect, but effect is slow, and be also required to mix to take with vitamin etc. just to have more effect, and need for a long time or
It is a large amount of edible, appetite certainly will be so influenceed, also easily disturbs the absorption and utilization of other nutrients.At present, it is on the market
Iron medicine, food and nutritional agents are mended, is by increasing the iron-holder in medicine, promoting to iron mostly in addition to expensive
Absorb, but after the molysite of organic acid largely takes in human body, the problems such as nutrient is normally taken in there is also interference body.This patent
Being compounded with chicken peptide and stevioside as base-material, the flavones ingredient in being revived using mountain promotes peptide iron construction stable,
The features such as biofermentation is increased operation rate, production are easy to the benefit iron richness peptide foodstuff base material absorbed by human body intestinal canal.
The content of the invention
In order to overcome the shortcomings and deficiencies of the prior art described above, primary and foremost purpose of the invention is to provide a kind of high-selenium corn
Rate mends the preparation method of iron richness peptide foodstuff base material.Benefit iron food prepared by this method using nature raw material glucosides peptide and iron from
Son carries out covalent bond, forms stable peptide iron construction, then the effect by mountain Soviet Union juice, forms the natural Targeting delivery effect of small intestine
Fruit;The characteristics of being easy to be absorbed by human body intestinal canal using the small peptide of biofermentation, by the peptide iron for forming rock-steady structure, you can increase
The strong flavor for mending iron foodstuff base material, can reach the effect by mending iron again.
Another object of the present invention is that providing the high-absorbility that a kind of above-mentioned preparation method is prepared mends iron richness peptide food
Base-material.
Still a further object of the present invention is to provide the application that a kind of above-mentioned high-absorbility mends iron richness peptide foodstuff base material.
The purpose of the present invention is realized by following proposal:
A kind of high-absorbility mends the preparation method of iron richness peptide foodstuff base material, including following operating procedure:
(1) using the Fresh Grade Breast meat gruel by wet pulverizing as raw material, by adding 20000u/g trypsase and 8000u/
G alkali proteases are hydrolyzed, and hydrolysis time is 3-5 hours, it is hydrolyzed to the chicken peptide that molecular weight is no more than 800Da;
(2) by stevioside according to weight than 1:3-1:5 ratio is well mixed with chicken peptide, using 300-500W
Ultrasonic wave discontinuity treatment 26-38 minutes, temperature maintains 38-42 DEG C all the time, and it is sub- that fumaric acid is added during ultrasonication
Iron, obtain mixed solution;The addition of ferrous fumarate is added according in every 100g steviosides and chicken peptide mixer
100-140mg ferrous fumarates;
(3) plant mountain soda comminution is starched, 200 eye mesh screen vacuum filters, juice is revived into according to 0.8- in filtering gained mountain
1.0wt% amount is added in the mixed solution of step (2), stirs 20-30 minutes by refiner, then penetrate by the way that temperature control is micro-
Flow homogeneous 1 time;
(4) 0.05-0.15wt% D-sorbite and 2-3wt% is added into the mixed liquor after homogeneous obtained by step (3)
PURE WHEY, then add Bacillus acidi lactici, 33-37 DEG C of temperature control, ferment after 3-5 hours, reach 10 to cell concentration3~
104Untill CFU/mL;Horizontal pulse of going forward side by side sterilizes, and obtains the rich peptide raw material that ferments;
(5) the rich peptide raw material that will ferment is spray-dried, and gained dry powder is that high-absorbility mends iron richness peptide foodstuff base material;
Pack.
The fineness of step (1) the Fresh Grade Breast meat gruel is 80-120 mesh.
Step (3) the temperature control microjet is control pressure 100-120MPa, 4-10 DEG C of temperature.
A kind of high-absorbility being prepared by above-mentioned preparation method mends iron richness peptide foodstuff base material.
Above-mentioned high-absorbility mends application of the iron richness peptide foodstuff base material in preparing high-absorbility and mending iron richness peptide meal replacement powder.
It is that mass percent 5-7% high-absorbility is mended into iron richness peptide food that the high-absorbility, which mends iron richness peptide meal replacement powder,
Base-material, mass percent 15-17% crystal diabetin, mass percent 12-16% maltodextrin, mass percent 13-
15% soybean protein isolate, mass percent 13-15% coarse rice powder, mass percent 7.5-8.5% whole milk powder,
Mass percent 6-8% walnut kernel, mass percent 3.5-5.5% FOSs, mass percent 3-4% medium chain triglyceride
Three cosmetics, mass percent 2.5-3.5% auricularia auriculajudae powder, vitamin C and vitamin E compounding are well mixed, pack.
The high-absorbility mends iron richness peptide meal replacement powder according to mass ratio 1:3 warm waters reconstitute edible.
Absorption to iron is strengthened by edible foodstuff base material of the present invention, reduces the various human bodies caused by hypoferric anemia
Disease or sub-health state, and change existing benefit iron food and mainly human body is brought not with increasing the technologies such as iron ion content
Profit influences.
The present invention is had the following advantages and beneficial effect relative to prior art:
(1) compared with traditional supplement iron product, the present invention is using the glucosides peptide of natural component with iron ion in ultrasonic wave
Effect is lower to carry out covalent bond, forms stable peptide-iron construction, then by the natural micro-embedded effect of mountain Soviet Union juice, formed stable
Small-particle polymer, be characterized in being oriented in small intestine Targeting delivery peptide iron conjugate, more complete peptide iron construction can be kept to make
It reaches intestinal absorption site;By the peptide iron for forming rock-steady structure, you can the flavor of iron foodstuff base material is mended in enhancing, again can be with
Reach the effect by mending iron.
(2) compared with traditional supplement ferro element product, first, raw material is raw material from the gentle chicken of property, is aided with
The natural material compositions such as STEVIA REBAUDIANA, mountain Soviet Union, nutritional ingredient collocation are reasonable;The long-term consumption such as breakfast can be substituted, by normal
Diet strengthens absorption of the human body to iron, no adverse side effect.
(3) the benefit iron richness Gly-His-Lys prepared using fermentation technique have the pleasant flavor of nature, and its flavor need not add people
Work spices reconciles, and it is more preferable to mend iron product quality than tradition, it is easier to child, old man etc. is received taste sensitive group.
Embodiment
With reference to embodiment, the present invention is described in further detail, but the implementation of the present invention is not limited to this.
Embodiment 1
(1) using the Fresh Grade Breast meat gruel by wet pulverizing as raw material (80 mesh), by adding trypsase (20000u/g)
It is hydrolyzed with alkali protease (8000u/g), hydrolysis time is 4 hours, it is hydrolyzed to molecular weight no more than 800Da's
Chicken peptide;
(2) by stevioside according to weight than 1:5 ratio is well mixed with chicken peptide, between ultrasonic wave (400W)
Disconnected processing 38 minutes, temperature maintains 40 DEG C all the time, and ferrous fumarate is added (according to every 100g STEVIA REBAUDIANAs in ultrasonication
100mg ferrous fumarates are added in glucosides and chicken peptide mixer), obtain mixed solution;
(3) plant mountain soda comminution is starched, 200 eye mesh screen vacuum filters, juice is revived into according to 1.0wt% in mountain after filtering
Add in mixed solution obtained by step (2), stirred 20 minutes by refiner, then pass through temperature control microjet homogeneous 1 time
(120MPa, 7 DEG C of temperature);
(4) 0.15wt% D-sorbite and 2.5 wt% breast are added into the mixed liquor after homogeneous obtained by step (3)
Albumin powder, then Bacillus acidi lactici is added, 37 DEG C of temperature control, ferment 3 hours, reach 10 to cell concentration4CFU/mL;And carry out arteries and veins
Punching sterilizing, obtains the rich peptide raw material that ferments;
(5) the rich peptide raw material that will ferment is spray-dried, and gained dry powder is that high-absorbility mends iron richness peptide foodstuff base material;
Pack.
(6) high-absorbility of obtained mass percent 5% is mended into iron richness peptide foodstuff base material, mass percent 16%
Crystal diabetin, the maltodextrin of mass percent 12%, the soybean protein isolate of mass percent 14%, mass percent
13% coarse rice powder, the whole milk powder of mass percent 8.5%, the walnut kernel of mass percent 7%, mass percent 3.5% are low
Fructooligosaccharides, the medium chain triglycerides powder of mass percent 4%, the auricularia auriculajudae powder of mass percent 3.5%, vitamin C and vitamin
E compoundings are well mixed, pack, and obtain high-absorbility and mend iron richness peptide meal replacement powder.The high-absorbility is mended iron richness peptide meal replacement powder and pressed
According to mass ratio 1:3 warm waters reconstitute edible.
Embodiment 2
(1) using the Fresh Grade Breast meat gruel by wet pulverizing as raw material (100 mesh), by adding trypsase (20000u/
G) it is hydrolyzed with alkali protease (8000u/g), hydrolysis time is 5 hours, it is hydrolyzed to molecular weight and is no more than 800Da
Chicken peptide;
(2) by stevioside according to weight ratio (1:4) it is well mixed with chicken peptide, in ultrasonic wave (300W) discontinuities
Reason 32 minutes, temperature maintains 38 DEG C all the time, and ferrous fumarate is added during ultrasonication (according to every 100g STEVIA REBAUDIANAs
120mg ferrous fumarates are added in glucosides and chicken peptide mixer), obtain mixed solution
(3) by plant mountain soda comminution starch, 200 eye mesh screen vacuum filters, by after filtering mountain revive juice according to
In the mixed solution of (0.8wt%) addition step (2), stirred 20 minutes by refiner, then pass through temperature control microjet homogeneous 1
Secondary (100MPa, 4 DEG C of temperature);
(4) 0.05wt% D-sorbite and 2 wt% whey are added into the mixed liquor after homogeneous obtained by step (3)
Albumen powder, then Bacillus acidi lactici is added, 33 DEG C of temperature control, ferment 4 hours, reach 10 to cell concentration3CFU/mL;Go forward side by side horizontal pulse
Sterilizing, obtains the rich peptide raw material that ferments;
(5) the rich peptide raw material that will ferment is spray-dried, and gained dry powder is that high-absorbility mends iron richness peptide foodstuff base material;
Pack.
(6) high-absorbility of obtained mass percent 6% is mended into iron richness peptide foodstuff base material, mass percent 15%
Crystal diabetin, the maltodextrin of mass percent 14%, the soybean protein isolate of mass percent 13%, mass percent
14% coarse rice powder, the whole milk powder of mass percent 7.5%, the walnut kernel of mass percent 8%, mass percent 5.5% are low
Fructooligosaccharides, the medium chain triglycerides powder of mass percent 3.5%, the auricularia auriculajudae powder of mass percent 2.5%, vitamin C and Wei Sheng
Plain E compoundings are well mixed, pack, and obtain high-absorbility and mend iron richness peptide meal replacement powder.The high-absorbility mends iron richness peptide meal replacement powder
According to mass ratio 1:3 warm waters reconstitute edible.
Embodiment 3
(1) using the Fresh Grade Breast meat gruel by wet pulverizing as raw material (120 mesh), by adding trypsase (20000u/
G) it is hydrolyzed with alkali protease (8000u/g), hydrolysis time is 3 hours, it is hydrolyzed to molecular weight and is no more than 800Da
Chicken peptide;
(2) by stevioside according to weight ratio (1:3) it is well mixed with chicken peptide, in ultrasonic wave (500W) discontinuities
Reason 26 minutes, temperature maintains 42 DEG C all the time, and ferrous fumarate is added during ultrasonication (according to every 100g STEVIA REBAUDIANAs
100-140mg ferrous fumarates are added in glucosides and chicken peptide mixer), obtain mixed solution;
(3) by plant mountain soda comminution starch, 200 eye mesh screen vacuum filters, by after filtering mountain revive juice according to
In the mixed solution of (0.9wt%) addition step (2), stirred 30 minutes by refiner, then pass through temperature control microjet homogeneous 1
Secondary (110MPa, 10 DEG C of temperature);
(4) 0.10wt% D-sorbite and 3 wt% whey are added into the mixed liquor after homogeneous obtained by step (3)
Albumen powder, then Bacillus acidi lactici is added, 35 DEG C of temperature control, ferment 5 hours, reach 10 to cell concentration4CFU/mL;Go forward side by side horizontal pulse
Sterilizing, obtains the rich peptide raw material that ferments;
(5) the rich peptide raw material that will ferment is spray-dried, and gained dry powder is that high-absorbility mends iron richness peptide foodstuff base material;
Pack.
(6) high-absorbility of obtained mass percent 7% is mended into iron richness peptide foodstuff base material, mass percent 17%
Crystal diabetin, the maltodextrin of mass percent 14%, the soybean protein isolate of mass percent 15%, mass percent
15% coarse rice powder, the whole milk powder of mass percent 8%, the walnut kernel of mass percent 6%, mass percent 4.5% are oligomeric
Fructose, the medium chain triglycerides powder of mass percent 3%, the auricularia auriculajudae powder of mass percent 3%, vitamin C and vitamin E are answered
With being well mixed, pack, obtain high-absorbility and mend iron richness peptide meal replacement powder.The high-absorbility mend iron richness peptide meal replacement powder according to
Mass ratio 1:3 warm waters reconstitute edible.
High-absorbility obtained by embodiment 1-3 is mended into iron richness peptide foodstuff base material, is referred to as mending iron richness Gly-His-Lys,
Performance detection is carried out, as a result as shown in table 1:
Absorptivity and its qualitative characteristics of the table 1Caco-2 cells to benefit iron richness Gly-His-Lys
Note:(1) Caco-2 cells absorptivity detection method:
Caco-2 cell models predict the progress of active material Absorption And Metabolism, 2015,15 (1):164-172. (2)
Flavor components detection method:(GC-MS gas-chromatography detection methods)
SPME-GC-MS detection [J] of the flavor components detection according to document Rapid Fermentation spun gold fish volatile flavor component,
Food and machinery, 2009,5 (6):102-106.
(3) method for detecting protein content:
The measure of GB 50095-2010 national food safety standard Protein in Food
(4)Fe2+Detection method of content:
Iron, zinc microelement field quick detection technical research, military medicine section of the Chinese People's Liberation Army in functional food
Institute, 2012, Master's thesis.
Above-described embodiment is the preferable embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any Spirit Essences without departing from the present invention with made under principle change, modification, replacement, combine, letter
Change, should be equivalent substitute mode, be included within protection scope of the present invention.
Claims (7)
1. a kind of high-absorbility mends the preparation method of iron richness peptide foodstuff base material, it is characterised in that including following operating procedure:
(1) using the Fresh Grade Breast meat gruel by wet pulverizing as raw material, by adding 20000u/g trypsase and 8000u/g alkalescence
Protease is hydrolyzed, and hydrolysis time is 3-5 hours, it is hydrolyzed to the chicken peptide that molecular weight is no more than 800Da;
(2) by stevioside according to weight than 1:3-1:5 ratio is well mixed with chicken peptide, using 300-500W ultrasound
Ripple discontinuity treatment 26-38 minutes, temperature maintain 38-42 DEG C, ferrous fumarate are being added during ultrasonication, is being obtained all the time
To mixed solution;The addition of ferrous fumarate is to add 100- according in every 100g steviosides and chicken peptide mixer
140mg ferrous fumarates;
(3) plant mountain soda comminution is starched, 200 eye mesh screen vacuum filters, juice is revived into according to 0.8-1.0wt% in filtering gained mountain
Amount be added in the mixed solution of step (2), 20-30 minutes are stirred by refiner, then pass through temperature control microjet homogeneous 1
It is secondary;
(4) 0.05-0.15wt% D-sorbite and 2-3wt% breast are added into the mixed liquor after homogeneous obtained by step (3)
Albumin powder, then Bacillus acidi lactici is added, 33-37 DEG C of temperature control, ferment after 3-5 hours, reach 10 to cell concentration3~104CFU/mL
Untill;Horizontal pulse of going forward side by side sterilizes, and obtains the rich peptide raw material that ferments;
(5) the rich peptide raw material that will ferment is spray-dried, and gained dry powder is that high-absorbility mends iron richness peptide foodstuff base material;Sealed bundle
Dress.
2. a kind of high-absorbility according to claim 1 mends the preparation method of iron richness peptide foodstuff base material, it is characterised in that:Step
Suddenly the fineness of (1) described Fresh Grade Breast meat gruel is 80-120 mesh.
3. a kind of high-absorbility according to claim 1 mends the preparation method of iron richness peptide foodstuff base material, it is characterised in that:Step
Suddenly (3) described temperature control microjet is control pressure 100-120MPa, 4-10 DEG C of temperature.
4. the high-absorbility that a kind of preparation method according to claim 1 is prepared mends iron richness peptide foodstuff base material.
5. high-absorbility according to claim 4 mends iron richness peptide foodstuff base material and is preparing high-absorbility benefit iron richness peptide meal replacement powder
In application.
6. application according to claim 5, it is characterised in that:It is by quality hundred that the high-absorbility, which mends iron richness peptide meal replacement powder,
Fraction 5-7% high-absorbility mends iron richness peptide foodstuff base material, mass percent 15-17% crystal diabetin, mass percent 12-
16% maltodextrin, mass percent 13-15% soybean protein isolate, mass percent 13-15% coarse rice powder, quality
Percentage 7.5-8.5% whole milk powder, mass percent 6-8% walnut kernel, mass percent 3.5-5.5% FOSs,
Mass percent 3-4% medium chain triglycerides powder, mass percent 2.5-3.5% auricularia auriculajudae powder, vitamin C and vitamin E
Compounding is well mixed, packs.
7. application according to claim 5, it is characterised in that:The high-absorbility mends iron richness peptide meal replacement powder according to mass ratio
1:3 warm waters reconstitute edible.
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