CN107744053A - A kind of Parent Female of Macrobrachium rosenbergii resisting stress promotees breeding pellet and preparation method thereof - Google Patents
A kind of Parent Female of Macrobrachium rosenbergii resisting stress promotees breeding pellet and preparation method thereof Download PDFInfo
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Abstract
The present invention relates to a kind of Parent Female of Macrobrachium rosenbergii resisting stress to promote breeding pellet and preparation method thereof, belongs to aquatic product matched field of feed.It first crushes fish meal, dregs of beans, cuttlefish powder, rapeseed meal, long patent flour, is stirred mixing with fish oil, potassium sorbate mould inhibitor, soybean lecithin powder, Choline Chloride, fishing premix, vitamin E, leaf of Moringa tunning, mulberry-leaf extract, Chinese rhubarb extract, zeolite powder and calcium dihydrogen phosphate etc. afterwards；It is mixed material to be crushed after stirring and evenly mixing by pulverizer, and pellet finally is made using the conventional method of pellet.Present invention preparation gained pellet nutrition is comprehensive, abundant, can improve Parent Female of Macrobrachium rosenbergii anti-stress ability, increase reproductive capacity, increase the survival rate of prelarva.
Description
Technical field
The present invention relates to a kind of Parent Female of Macrobrachium rosenbergii resisting stress to promote breeding pellet and preparation method thereof, belongs to aquatic products and matches somebody with somebody
Close field of feed.
Background technology
Macrobrachium rosenbergii（Macrobrachium rosenbergii）Also known as the long-armed prawn of Malaysian prawn, fresh water, money
Shrimp, big river prawn, for Freshwater shrimps maximum in the world, it is northern and western to originate in whole South Asia and south east asia and Oceania
Pacific Islands, whole year is lived in these regional fresh water or salt-fresh water waters, and is entered in the Hekou Area influenceed by tidewater
Row breeding.The Macrobrachium rosenbergii bodily form is larger, feeding habits are wide, disease is few, easy existence, growth is fast, nutrition is good, therefore has important economy
Value.
The feed nutrition of Parent Female of Macrobrachium rosenbergii grows to egg laying amount, ovum and the young shrimp quality and young shrimp that improve close shrimp, survived
Rate etc. has a major impact.The quality of close shrimp directly affects the quality of seed, influences the cultivation growth of Macrobrachium rosenbergii.In Roche
Low when high during the yield of many nurseries in pond crayfish nursery production process, bad when good during fry quality, production is very unstable, makes
On the other hand it is Broodstock Rearing Technology of Macrobracium and battalion into being on the one hand outside larval culture technology and its open-mouthed bait the reason for this phenomenon
It is relevant to support reinforcing, causes the of poor quality or lazy weight of oogenesis parent shrimp, normal nursery production is have impact on, after particularly educating
Phase, the reproduction ability of seed shrimp are gradually reduced.General seedling field uses common Macrobrachium rosenbergii commercial feed, some spiral shells of arranging in pairs or groups in recent years
The natural baits such as silk, chilled fish, one side feed waste is big, easy polluted-water, the natural bait such as another aspect screw, chilled fish
Cause of disease, which may be carried, causes seed shrimp, at the same carry cause of disease may also by vertical transmission to seedling, cause close shrimp spawning less,
Low reproduction rate, the survival rate of fry are low.
A kind of very common physiological status of stressed animal, it is that animal body produces to external environment or body interior
The summation for the various physiologic responses reaction made during raw abnormal stimulation.This is the natural reaction of animal, dynamic in stress situation
Thing tends not to normal growth and breeding.Aquatic livestock, including fish, Shrimp waste and shellfish etc. can all produce stress reaction.Root
From the point of view of the nursery period of Macrobrachium rosenbergii, start nearly seed shrimp or so November and enter indoor warmhouse booth cultivation, carry out fortification 2-3
Individual month, or so the March in the coming year formal numerous seedling.This process Macrobrachium rosenbergii seed shrimp persistently undergoes transport, temperature change, hypoxemia, close
The stress factors such as degree, reproductive performance is reduced, disease, even death easily occurs.How to avoid Parent Female of Macrobrachium rosenbergii produce stress
Negative interaction is that raising breeding efficiency and the highly important technology of productivity effect will.
More than understand, in seedling raising process Macrobrachium rosenbergii seed shrimp be subjected to environmental stress, food nutrition lack etc. it is many should
Swash stress, cause close shrimp to lay eggs less, low reproduction rate, the survival rate of fry is low, while also results in cultivation offspring's fry quality and pay no attention to
Topic, brings the industry problems such as diseases such as " iron-clad shrimps " is more, and cultivation risk is big, is unfavorable for the sound development of the industry of Macrobrachium rosenbergii.
For these problems, flood gets over group etc.（2016）Apply for a patent（201610010269.7）A kind of Parent Female of Macrobrachium rosenbergii pellet and
Its preparation method, mainly balanced by conventional nutrient, improve the efficiency of feed utilization of shrimp, improve shrimp reproductive performance.Wang Jiqiao
（2002）The influence that stress stimulation is bred to cultured fishes is elaborated, such as：Agitation or operation can make rainbow trout in advance or postpone breeding,
Also it can accelerate or completely inhibit bolti breeding, these stressors can be influenceed numerous by changing the quality of gamete or offspring
Grow.Wang Minjie etc. (2016）Apply for a patent（201611090050.9）Macrobrachium rosenbergii is disease-resistant and the plumage of anti-stress ability for a kind of enhancing
Hair hydrolysis powder feed and preparation method thereof.Yuan Ye etc.（2016）Apply for a patent（201610151676.X）A kind of Macrobrachium rosenbergii
Resisting stress reinforcing agent and its application.But stress be also rare with the paper and patent of breeding about improving shrimp Macrobrachium rosenbergii seed shrimp,
It can improve that seed shrimp is immune and anti-stress ability, be favorably improved the pellet of reproductive performance and its prepare application method, also not
Appear in the newspapers.Therefore, this patent is directed to the feed of Parent Female of Macrobrachium rosenbergii, from the angle of fish body breeding, improves Parent Female of Macrobrachium rosenbergii
Fertility, the immune and anti-anti-stress ability of Parent Female of Macrobrachium rosenbergii on the other hand can be improved, strengthens resistance against diseases, exploitation is a kind of
There is provided nutrition comprehensive, abundant, balance mixed feed.
The content of the invention
It is an object of the present invention to overcome the above deficiencies, there is provided a kind of Parent Female of Macrobrachium rosenbergii resisting stress promotees breeding particle
Feed and preparation method thereof, its feed nutrition is comprehensive, abundant, improves the anti-stress ability of Parent Female of Macrobrachium rosenbergii, enhancing breeding energy
Power, be advantageous to breeding period nutritional need and growth.
According to technical scheme provided by the invention, Parent Female of Macrobrachium rosenbergii resisting stress promotees breeding pellet, and formula rate is pressed
Parts by weight meter is as follows：Fish meal 32.0-35.0 parts, cuttlefish powder 3.0-5.0 parts, shrimp med 6.0-8.0 parts, 20.0 parts of dregs of beans 18.0-,
Rapeseed meal 9.0-12.0 parts, long patent flour 11.5-13.0 parts, fish oil 5.0-7.0 parts, soybean lecithin powder 0.50-1.20 parts, chlorination courage
Alkali 0.5-1.2 parts, calcium dihydrogen phosphate 2.10-2.50 parts, shell element 0.2-0.4 parts, potassium sorbate mould inhibitor 0.2-0.4 parts, boiling
Stone flour 0.84-1.07 parts, fishing premix 0.9-1.2 parts, vitamin E 0.06-0.15 parts, vitamin A 0.3-0.5 parts, L-
Methionine 0.3-0.5 parts, glycyl-L-glutamine 0.001-0.003 parts, leaf of Moringa tunning 0.025-0.075 parts, mulberry
Leaf extract 0.002-0.004 parts, Chinese rhubarb extract 0.001-0.003 parts；
First by fish meal, dregs of beans, cuttlefish powder, rapeseed meal, long patent flour crush, afterwards with fish oil, potassium sorbate mould inhibitor, soybean lecithin
Powder, Choline Chloride, fishing premix, vitamin E, leaf of Moringa tunning, mulberry-leaf extract, Chinese rhubarb extract, zeolite powder and
Calcium dihydrogen phosphate etc. is stirred mixing；It is mixed material to be crushed after stirring and evenly mixing by pulverizer, finally utilizes pellet
Conventional method be made Parent Female of Macrobrachium rosenbergii resisting stress promote breeding pellet.
The Parent Female of Macrobrachium rosenbergii resisting stress promotees the preparation method of breeding pellet, and step by weight is as follows：
（1）Mixed once is with crushing：By fish meal 32.0-35.0 parts, cuttlefish powder 3.0-5.0 parts, shrimp med 6.0-8.0 parts, dregs of beans
First time crushing, powder are carried out in 20.0 parts of 18.0-, rapeseed meal 9.0-12.0 parts and long patent flour 11.5-13.0 parts input pulverizer
Broken speed is 400-500r/min, obtains mixing thick material, and cross 40-60 eye mesh screens；
（2）Two batch mixings：In step（1）Gained, which mixes, adds fish oil 5.0-7.0 parts, soybean lecithin powder 0.50-1.20 in thick material
Part, Choline Chloride 0.5-1.2 parts, calcium dihydrogen phosphate 2.10-2.50 parts, shell element 0.2-0.4 parts, potassium sorbate mould inhibitor 0.2-
0.4 part and zeolite powder 0.84-1.07 parts, put into mixer and be well mixed, mixer rotating speed is 18-20r/min when feeding intake；
（3）Separating twice：Take step（2）Second is carried out in the well mixed material input pulverizer of gained to crush, and is passed through
40-60 eye mesh screens；Pulverizer crushes speed, and crushing speed is 400-500r/min；
（4）Batch mixing three times：In step（3）Gained, which mixes, adds fishing premix 0.9-1.2 parts, vitamin E in thick material
0.06-0.15 parts, vitamin A 0.3-0.5 parts, L-Methionine 0.3-0.5 parts, glycyl-L-glutamine 0.001-0.003
Part, leaf of Moringa tunning 0.025-0.075 parts, mulberry-leaf extract 0.002-0.004 parts and Chinese rhubarb extract 0.001-0.003
Part, put into mixer and be well mixed, mixer rotating speed is 18-20r/min when feeding intake；
（5）Crush three times：Take step（4）Third time crushing is carried out in the well mixed material input pulverizer of gained, and is passed through
40-60 eye mesh screens；Pulverizer crushes speed, and crushing speed is 400-500r/min；
（6）Post processing：Take step（5）Mixed material input aquatic feeds granulator after obtained separating twice, is made particle
Feed；Pellet is inserted in drying machine and dried at a temperature of 90-100 DEG C；By the pellet after drying in cooler
Packed after cooling, produce product Parent Female of Macrobrachium rosenbergii resisting stress and promote breeding pellet.
Step（4）The leaf of Moringa tunning preparation method is：Take leaf of Moringa to smash to 40-80 mesh, leaf of Moringa is made
Powder；It is added in the aqueous solution that pH is 4.0-6.0, leaf of Moringa and water quality ratio are 8-12:1, it is placed in fermentation tank, simultaneously
Bacillus amyloliquefaciens are added in fermentation tank, it is 30-40 DEG C, mixing speed 4-8r/min to set fermentation tank tank temperature, culture
16-32h；The zymotic fluid of gained is poured into the volumetric flask of Rotary Evaporators, it is 50-55 DEG C to set temperature, air pressure 0.001-
0.003MPa, extraction 2 ~ 4 times being repeated, 2 ~ 4 gained are screwed out into liquid concentrates in Rotary Evaporators, and thickening temperature is 70-80 DEG C,
Air pressure is 0.001-0.003MPa, untill being concentrated into stiff paste form, produces the leaf of Moringa tunning.
The aqueous solution that the pH is 4.0-6.0 uses Acetic acid-sodium acetate as pH adjusting agent；The bacillus amyloliquefaciens
FFRC-S24 is preserved in China on May 9th, 2013 and establishes culture collection, and deposit number is：CCTCC NO：
M2013191, specification are 10,000,000,000 CFU/g.
The pellet particle diameter is 1.0-3.0mm.
Beneficial effects of the present invention：
1st, mulberry-leaf extract, vitamin E, vitamin A etc. are with the addition of in mixed feed can increase Parent Female of Macrobrachium rosenbergii spawning, improve
Fertility.
2nd, leaf of Moringa tunning, glycyl-L-glutamine, Chinese rhubarb extract are with the addition of in mixed feed, rheum officinale carries
Take the immunopotentiators such as thing, the anti-stress ability of shrimp can be strengthened, reduce stress harm.
3rd, it is soybean lecithin powder, Choline Chloride and L-Methionine that this pellet, which with the addition of content, can improve energy utilization
Rate, mitigate stress be with, because of the deficiency disease of methionine requirement deficiency, improving efficiency of feed utilization and fish liver fat under Reproduction Conditions
The metabolism of fat, reduces the generation of fish body fatty liver, and has certain attractant effect, increases palatability, advantageously reduces bait
Expect coefficient, improve its growth.
4th, sufficient raw selected by this patent feed formula, the technique mixed three times with crushing three times is employed, is ensured
The grinding particle size and mixture homogeneity of feedstuff, stability of the increase shrimp material in water, there is provided efficiency of feed utilization.
5th, the nutrition of this pellet is comprehensive, abundant, compared with the Parent Female of Macrobrachium rosenbergii bait such as traditional screw, chilled fish,
Anti-stress ability can be improved, increases fish brood amount, Macrobrachium rosenbergii reproductive capacity is improved, increases young shrimp survival rate.
Embodiment
It is flavonoids, alkaloid, mulberry leaf polysaccharide etc. that mulberry-leaf extract described in following examples, which has Main Ingredients and Appearance, polysaccharide
10%-30%, provided by bright biological development corporation, Ltd. of Baoji side.Described glycyl-L-glutamine, content 98%, by
Zhejiang Quzhou Sai Muzhong spaces Bioisystech Co., Ltd provides.Rheum emodin, content 98%, had by the huge nation's plant material in Shifang City
Limit company provides.Described L-Methionine is provided by Zhengzhou Fu Run moral bioengineering Co., Ltd for you.Described Choline Chloride
Content is 50%, and by Hebei, global feed addictive factory provides.Described shell element, by Shandong Hua Wei bio tech ltd
There is provided.Described potassium sorbate mould inhibitor is provided by Ningbo Wang Long Science and Technology Ltd.s.Described vitamin E, it is 50% vitamin
E acetates, provided by Zhejiang Dong Gong pharmaceutical Co. Ltds.Described vitamin A, 500000U/ grams of content, by Zhejiang east work system
Medicine Co., Ltd provides.Described soybean lecithin powder is provided by Zibo Qi Xing feed corporation,Ltds.Described fishing is with premix
The 1% fresh-water fishes premix containing multivitamin and mineral element, provided by Nowe animal health Co., Ltd of Wuxi China.
The present invention will be further described in conjunction with the embodiments below：
A kind of Parent Female of Macrobrachium rosenbergii resisting stress of embodiment 1 promotees breeding pellet and preparation method thereof, is walked using following technique
Suddenly：
Its each raw material composition of 100 kg feeds of present invention preparation：
35.0 kg fish meal, 3.0 kg cuttlefish powders, 8.0 kg shrimp meds, 18.0 kg dregs of beans, 10.0 kg rapeseed meals, the common faces of 11.7 kg
Powder, 7.0 kg fish oil, 0.5 kg soybean lecithins powder, 0.5 kg Choline Chlorides, 2.5kg calcium dihydrogen phosphates, 0.2 kg shells element,
0.3kg potassium sorbates mould inhibitor, 0.84kg zeolite powders, 1.0 kg fishing premixes, 0.05 kg vitamin Es, 0.3 kg vitamins
A, 0.5 kg L-Methionines, 0.002 kg glycyl-L-glutamines, 0.05kg leaf of Moringa tunning, 0.003 kg mulberry leaf
Extract, 0.003 kg Chinese rhubarb extracts.
（1）Mixed once is with crushing：First by fish meal, dregs of beans, silkworm chrysalis, rapeseed meal, cotton dregs, long patent flour, wheat-middlings, wash rice bran,
Potassium sorbate mould inhibitor is crushed to mix thick material for the first time through pulverizer, and crushing speed is 400r/min, and passes through 40 mesh sieves
Net；
（2）Two batch mixings：Thick material and fish oil, soybean lecithin powder, Choline Chloride, calcium dihydrogen phosphate, shell element, sorb will be mixed again
Sour potassium mould inhibitor, zeolite powder etc. are stirred in mixer（V-Mixer, Liyang Huida Machinery Co., Ltd.）It is two after interior mixing
Secondary mixed material；Mixer feeds intake rotating speed：18 revs/min；
（3）Separating twice：By second of crushing of pulverizer, and pass through 40 eye mesh screens；Pulverizer crushes speed：400 revs/min；
（4）Batch mixing three times：Mixed material is then added into vitamine supplement, mineral additive, vitamin E, vitamin
A, the stirring such as L-Methionine, glutamine, leaf of Moringa tunning, mulberry-leaf extract, Chinese rhubarb extract is in mixer（V-type is mixed
Conjunction machine, Liyang Huida Machinery Co., Ltd.）It is mixed material three times after interior mixing；Mixer feeds intake rotating speed：18 revs/min；
（5）Crush three times：Crushed by pulverizer third time, and pass through 40 eye mesh screens；Pulverizer crushes speed：400 revs/min；
（6）Post processing：Finally by the mixed material of gained aquatic feeds granulator（STZG series, Zheng Chang group companies）It is made
Feed granules.Feed granules particle diameter：1.0mm；Afterwards by feed granules in drying machine（DWT series band driers, Zheng Chang groups are public
Department）In dry at a temperature of 95 DEG C；By the expanded pellet diet after drying in cooler（SKLG900 roller coolers, Zheng Chang groups
Company）Packed after cooling.
The leaf of Moringa tunning preparation method is：Leaf of Moringa is smashed to 40 mesh, leaf of Moringa powder is made；It is added to
PH is in 4.0 aqueous solution, and leaf of Moringa is 8 with water quality ratio:1, it is placed in fermentation tank, while Xie Dian is added in fermentation tank
Afnyloliquefaciens（FFRC-S24）, it is 30 DEG C to set fermentation tank tank temperature, and mixing speed is 4 revs/min, cultivates 16h；By gained
Zymotic fluid is poured into the volumetric flask of Rotary Evaporators, and it is 50 DEG C, air pressure 0.001MPa to set temperature, repeats extraction three times, will
The back-out liquid of gained concentrates in Rotary Evaporators three times, and thickening temperature is 70 DEG C, air pressure 0.001MPa, is concentrated into thick cream
Untill shape thing, the leaf of Moringa tunning is produced.The aqueous solution that the pH is 4.0 uses Acetic acid-sodium acetate as pH adjusting agent；
The bacillus amyloliquefaciens（FFRC-S24）China is preserved on May 9th, 2013 and establishes culture collection, preservation is compiled
Number it is：CCTCC NO：M2013191, specification are 10,000,000,000 CFU/g.
Culture experiment：Macrobrachium rosenbergii is provided by Jiangsu Yangzhou Macrobrachium rosenbergii breeding company.Experiment selection health, specification are basic
Consistent Parent Female of Macrobrachium rosenbergii, random packet, provided with control group and test group, wherein control group without leaf of Moringa tunning,
Mulberry-leaf extract.Test group contains that the Parent Female of Macrobrachium rosenbergii such as 0.05% leaf of Moringa tunning and 0.002% mulberry-leaf extract are anti-should
Swash and promote breeding pellet.Every group sets 3 parallel tests, every tail shrimp of parallel group 30, totally 6 cement pits（Specification be 200cm ×
150cm×100cm）.Control group basal diet is fed after Macrobrachium rosenbergii domestication 15d and resisting stress promotees breeding particle test feed 8
In week, determine the indexs such as its anti-stress ability, fish brood amount, reproductive capacity, survival rate.
As a result show：Compared with control group, resisting stress promotees breeding particle test group survival rate and has been respectively increased 6.58%, shows
Writing reduces concentration of malondialdehyde 8.77%, adds catalase 11.34%, superoxide dismutase activity 7.58%, and improve
Fish brood amount 16.78%, increase the survival rate 9.89% of prelarva.Therefore, the resisting stress promotees breeding pellet culture experiment and shows energy
Increase the anti-stress ability and fertility of Macrobrachium rosenbergii.
A kind of Parent Female of Macrobrachium rosenbergii resisting stress of the present invention of embodiment 2 promotees breeding pellet and preparation method thereof, uses
Following processing step：
Its each raw material composition of 100 kg feeds of present invention preparation：
32.0 kg fish meal, 4.0 kg cuttlefish powders, 6.0 kg shrimp meds, 20.0 kg dregs of beans, 12.0 kg rapeseed meals, the common faces of 11.5 kg
Powder, 6.0 kg fish oil, 1.0 kg soybean lecithins powder, 1.0kg Choline Chlorides, 2.1kg calcium dihydrogen phosphates, 0.3kg shells element,
0.2kg potassium sorbates mould inhibitor, 1.07kg zeolite powders, 1.2kg fishing premix, 0.15kg vitamin Es, 0.5kg vitamin As,
0.4 kg L-Methionines, 0.001 kg glycyl-L-glutamines, 0.075kg leaf of Moringa tunning, 0.002 kg mulberry leaf
Extract, 0.002 kg Chinese rhubarb extracts.
（1）Mixed once is with crushing：First by fish meal, dregs of beans, silkworm chrysalis, rapeseed meal, cotton dregs, long patent flour, wheat-middlings, wash rice bran,
Potassium sorbate mould inhibitor is crushed to mix thick material for the first time through pulverizer, and crushing speed is 500r/min, and passes through 50 mesh sieves
Net；
（2）Two batch mixings：Thick material and fish oil, soybean lecithin powder, Choline Chloride, calcium dihydrogen phosphate, shell element, sorb will be mixed again
Sour potassium mould inhibitor, zeolite powder etc. are stirred in mixer（V-Mixer, Liyang Huida Machinery Co., Ltd.）It is two after interior mixing
Secondary mixed material；Mixer feeds intake rotating speed：19 revs/min；
（3）Separating twice：By second of crushing of pulverizer, and pass through 40 eye mesh screens；Pulverizer crushes speed：450 revs/min；
（4）Batch mixing three times：Mixed material is then added into vitamine supplement, mineral additive, vitamin E, vitamin
A, the stirring such as L-Methionine, glutamine, leaf of Moringa tunning, mulberry-leaf extract, Chinese rhubarb extract is in mixer（V-type is mixed
Conjunction machine, Liyang Huida Machinery Co., Ltd.）It is mixed material three times after interior mixing；Mixer feeds intake rotating speed：19 revs/min；
（5）Crush three times：Crushed by pulverizer third time, and pass through 40 eye mesh screens；Pulverizer crushes speed：450 revs/min；
（6）Post processing：Finally by the mixed material of gained aquatic feeds granulator（STZG series, Zheng Chang group companies）It is made
Feed granules.Feed granules particle diameter：2.0mm；Afterwards by feed granules in drying machine（DWT series band driers, Zheng Chang groups are public
Department）In dry at a temperature of 95 DEG C；By the expanded pellet diet after drying in cooler（SKLG900 roller coolers, Zheng Chang groups
Company）Packed after cooling.
The leaf of Moringa tunning preparation method is：Leaf of Moringa is smashed to 60 mesh, leaf of Moringa powder is made；It is added to
PH is in 5.0 aqueous solution, and leaf of Moringa is 10 with water quality ratio:1, it is placed in fermentation tank, while solution is added in fermentation tank
Bacillus amyloliquefacienses（FFRC-S24）, it is 35 DEG C to set fermentation tank tank temperature, and mixing speed is 6 revs/min, cultivates 24h；By gained
Zymotic fluid pour into the volumetric flask of Rotary Evaporators, set temperature be 52 DEG C, air pressure 0.002MPa, repeat extraction three times,
The back-out liquid of gained three times is concentrated in Rotary Evaporators, thickening temperature is 75 DEG C, air pressure 0.002MPa, is concentrated into thick
Untill paste, the leaf of Moringa tunning is produced.The aqueous solution that the pH is 5.0 uses Acetic acid-sodium acetate to be adjusted for pH
Agent；The bacillus amyloliquefaciens（FFRC-S24）China is preserved on May 9th, 2013 and establishes culture collection, is protected
Hiding numbering is：CCTCC NO：M2013191, specification are 10,000,000,000 CFU/g.
Culture experiment：Macrobrachium rosenbergii is provided by Jiangsu Yangzhou Macrobrachium rosenbergii breeding company.Experiment selection health, specification are basic
Consistent Parent Female of Macrobrachium rosenbergii, random packet, provided with control group and test group, wherein control group without leaf of Moringa tunning,
Mulberry-leaf extract.Test group contains that the Parent Female of Macrobrachium rosenbergii such as 0.025% leaf of Moringa tunning, 0.003% mulberry-leaf extract are anti-should
Swash and promote breeding pellet.Every group sets 3 parallel tests, every tail shrimp of parallel group 30, totally 6 cement pits（Specification be 200cm ×
150cm×100cm）.Control group basal diet is fed after Macrobrachium rosenbergii domestication 15d and resisting stress promotees breeding particle test feed 8
In week, determine the indexs such as its anti-stress ability, fish brood amount, reproductive capacity, survival rate.
As a result show：Compared with control group, resisting stress promotees breeding particle test group survival rate and has been respectively increased 7.34%, shows
Writing reduces concentration of malondialdehyde 9.82%, adds hydrogen peroxidase 9 .11%, superoxide dismutase activity 13.32%, and improve
Fish brood amount 15.88%, increase the survival rate 12.26% of prelarva.Therefore, the resisting stress promotees breeding pellet culture experiment and shows energy
Increase the anti-stress ability and fertility of Macrobrachium rosenbergii.
A kind of Parent Female of Macrobrachium rosenbergii resisting stress of the present invention of embodiment 3 promotees breeding pellet and preparation method thereof, uses
Following processing step：
Its each raw material composition of 100 kg feeds of present invention preparation：
33.0 kg fish meal, 5.0 kg cuttlefish powders, 7.0 kg shrimp meds, 19.0 kg dregs of beans, 9.0 kg rapeseed meals, the common faces of 13.0 kg
Powder, 5.0 kg fish oil, 1.2 kg soybean lecithins powder, 1.2 kg Choline Chlorides, 2.0 kg calcium dihydrogen phosphates, 0.4 kg shells element,
0.4 kg potassium sorbates mould inhibitor, 1.12 kg zeolite powders, 0.9kg fishing premix, 0.10 kg vitamin Es, 0.4 kg dimension
Raw plain A, 0.3 kg L-Methionines, 0.003 kg glycyl-L-glutamines, 0.025kg leaf of Moringa tunning, 0.004
Kg mulberry-leaf extracts, 0.001 kg Chinese rhubarb extracts.
（1）Mixed once is with crushing：First by fish meal, dregs of beans, silkworm chrysalis, rapeseed meal, cotton dregs, long patent flour, wheat-middlings, wash rice bran,
Potassium sorbate mould inhibitor is crushed to mix thick material for the first time through pulverizer, and crushing speed is 450r/min, and passes through 60 mesh sieves
Net；
（2）Two batch mixings：Thick material and fish oil, soybean lecithin powder, Choline Chloride, calcium dihydrogen phosphate, shell element, sorb will be mixed again
Sour potassium mould inhibitor, zeolite powder etc. are stirred in mixer（V-Mixer, Liyang Huida Machinery Co., Ltd.）It is two after interior mixing
Secondary mixed material；Mixer feeds intake rotating speed：20 revs/min；
（3）Separating twice：By second of crushing of pulverizer, and pass through 40 eye mesh screens；Pulverizer crushes speed：500 revs/min；
（4）Batch mixing three times：Mixed material is then added into vitamine supplement, mineral additive, vitamin E, vitamin
A, the stirring such as L-Methionine, glutamine, leaf of Moringa tunning, mulberry-leaf extract, Chinese rhubarb extract is in mixer（V-type is mixed
Conjunction machine, Liyang Huida Machinery Co., Ltd.）It is mixed material three times after interior mixing；Mixer feeds intake rotating speed：20 revs/min；
（5）Crush three times：Crushed by pulverizer third time, and pass through 40 eye mesh screens；Pulverizer crushes speed：500 revs/min；
（6）Post processing：Finally by the mixed material of gained aquatic feeds granulator（STZG series, Zheng Chang group companies）It is made
Feed granules.Feed granules particle diameter：3.0 mm；Afterwards by feed granules in drying machine（DWT series band driers, Zheng Chang groups
Company）In dry at a temperature of 95 DEG C；By the expanded pellet diet after drying in cooler（SKLG900 roller coolers, Zheng Changji
Company of group）Packed after cooling.
The leaf of Moringa tunning preparation method is：Leaf of Moringa is smashed to 80 mesh, leaf of Moringa powder is made；It is added to
PH is in 6.0 aqueous solution, and leaf of Moringa is 12 with water quality ratio:1, it is placed in fermentation tank, while solution is added in fermentation tank
Bacillus amyloliquefacienses（FFRC-S24）, it is 40 DEG C to set fermentation tank tank temperature, and mixing speed is 8 revs/min, cultivates 32h；By gained
Zymotic fluid pour into the volumetric flask of Rotary Evaporators, set temperature be 55 DEG C, air pressure 0.003MPa, repeat extraction three times,
The back-out liquid of gained three times is concentrated in Rotary Evaporators, thickening temperature is 80 DEG C, air pressure 0.003MPa, is concentrated into thick
Untill paste, the leaf of Moringa tunning is produced.The aqueous solution that the pH is 6.0 uses Acetic acid-sodium acetate to be adjusted for pH
Agent；The bacillus amyloliquefaciens（FFRC-S24）China is preserved on May 9th, 2013 and establishes culture collection, is protected
Hiding numbering is：CCTCC NO：M2013191, specification are 10,000,000,000 CFU/g.
Culture experiment：Macrobrachium rosenbergii is provided by Jiangsu Yangzhou Macrobrachium rosenbergii breeding company.Experiment selection health, specification are basic
Consistent Parent Female of Macrobrachium rosenbergii, random packet, provided with control group and test group, wherein control group without leaf of Moringa tunning,
Mulberry-leaf extract.Test group contains 0.075% leaf of Moringa tunning, the Parent Female of Macrobrachium rosenbergii of 0.004% mulberry-leaf extract resists should
Swash and promote breeding pellet.Every group sets 3 parallel tests, every tail shrimp of parallel group 30, totally 6 cement pits（Specification be 200cm ×
150cm×100cm）.Control group basal diet is fed after Macrobrachium rosenbergii domestication 15d and resisting stress promotees breeding particle test feed 8
In week, determine the indexs such as its anti-stress ability, fish brood amount, reproductive capacity, survival rate.
As a result show：Compared with control group, resisting stress promotees breeding particle test group survival rate and has been respectively increased 8.87%, shows
Writing reduces concentration of malondialdehyde 10.21%, adds catalase 7.94%, superoxide dismutase activity 13.61%, and carry
High fish brood amount 14.76%, increase the survival rate 11.23% of prelarva.Therefore, resisting stress rush breeding pellet culture experiment shows
The anti-stress ability and fertility of Macrobrachium rosenbergii can be increased.
Claims (5)
1. Parent Female of Macrobrachium rosenbergii resisting stress promotees breeding pellet, it is characterized in that formula rate is as follows by weight：Fish meal
It is 32.0-35.0 parts, cuttlefish powder 3.0-5.0 parts, shrimp med 6.0-8.0 parts, 20.0 parts of dregs of beans 18.0-, rapeseed meal 9.0-12.0 parts, general
Logical flour 11.5-13.0 parts, fish oil 5.0-7.0 parts, soybean lecithin powder 0.50-1.20 parts, Choline Chloride 0.5-1.2 parts, phosphoric acid
Calcium dihydrogen 2.10-2.50 parts, shell element 0.2-0.4 parts, potassium sorbate mould inhibitor 0.2-0.4 parts, zeolite powder 0.84-1.07 parts,
Fishing with premix 0.9-1.2 parts, vitamin E 0.06-0.15 parts, vitamin A 0.3-0.5 parts, L-Methionine 0.3-0.5 parts,
Glycyl-L-glutamine 0.001-0.003 parts, leaf of Moringa tunning 0.025-0.075 parts, mulberry-leaf extract 0.002-
0.004 part, Chinese rhubarb extract 0.001-0.003 parts；
First by fish meal, dregs of beans, cuttlefish powder, rapeseed meal, long patent flour crush, afterwards with fish oil, potassium sorbate mould inhibitor, soybean lecithin
Powder, Choline Chloride, fishing premix, vitamin E, leaf of Moringa tunning, mulberry-leaf extract, Chinese rhubarb extract, zeolite powder and
Calcium dihydrogen phosphate etc. is stirred mixing；It is mixed material to be crushed after stirring and evenly mixing by pulverizer, finally utilizes pellet
Conventional method be made Parent Female of Macrobrachium rosenbergii resisting stress promote breeding pellet.
2. Parent Female of Macrobrachium rosenbergii resisting stress described in claim 1 promotees the preparation method of breeding pellet, it is characterized in that by weight
Part meter step is as follows：
（1）Mixed once is with crushing：By fish meal 32.0-35.0 parts, cuttlefish powder 3.0-5.0 parts, shrimp med 6.0-8.0 parts, dregs of beans
First time crushing, powder are carried out in 20.0 parts of 18.0-, rapeseed meal 9.0-12.0 parts and long patent flour 11.5-13.0 parts input pulverizer
Broken speed is 400-500r/min, obtains mixing thick material, and cross 40-60 eye mesh screens；
（2）Two batch mixings：In step（1）Gained, which mixes, adds fish oil 5.0-7.0 parts, soybean lecithin powder 0.50-1.20 in thick material
Part, Choline Chloride 0.5-1.2 parts, calcium dihydrogen phosphate 2.10-2.50 parts, shell element 0.2-0.4 parts, potassium sorbate mould inhibitor 0.2-
0.4 part and zeolite powder 0.84-1.07 parts, put into mixer and be well mixed, mixer rotating speed is 18-20r/min when feeding intake；
（3）Separating twice：Take step（2）Second is carried out in the well mixed material input pulverizer of gained to crush, and is passed through
40-60 eye mesh screens；Pulverizer crushes speed, and crushing speed is 400-500r/min；
（4）Batch mixing three times：In step（3）Gained, which mixes, adds fishing premix 0.9-1.2 parts, vitamin E in thick material
0.06-0.15 parts, vitamin A 0.3-0.5 parts, L-Methionine 0.3-0.5 parts, glycyl-L-glutamine 0.001-0.003
Part, leaf of Moringa tunning 0.025-0.075 parts, mulberry-leaf extract 0.002-0.004 parts and Chinese rhubarb extract 0.001-0.003
Part, put into mixer and be well mixed, mixer rotating speed is 18-20r/min when feeding intake；
（5）Crush three times：Take step（4）Third time crushing is carried out in the well mixed material input pulverizer of gained, and is passed through
40-60 eye mesh screens；Pulverizer crushes speed, and crushing speed is 400-500r/min；
（6）Post processing：Take step（5）Mixed material input aquatic feeds granulator after obtained separating twice, is made particle
Feed；Pellet is inserted in drying machine and dried at a temperature of 90-100 DEG C；By the pellet after drying in cooler
Packed after cooling, produce product Parent Female of Macrobrachium rosenbergii resisting stress and promote breeding pellet.
3. Parent Female of Macrobrachium rosenbergii resisting stress described in claim 2 promotees the preparation method of breeding pellet, it is characterized in that step（4）
The leaf of Moringa tunning preparation process is：Take leaf of Moringa to smash to 40-80 mesh, leaf of Moringa powder is made；Being added to pH is
In the 4.0-6.0 aqueous solution, leaf of Moringa and water quality ratio are 8-12:1, it is placed in fermentation tank, while added in fermentation tank
Bacillus amyloliquefaciens, it is 30-40 DEG C, mixing speed 4-8r/min to set fermentation tank tank temperature, cultivates 16-32h；By gained
Zymotic fluid is poured into the volumetric flask of Rotary Evaporators, and it is 50-55 DEG C, air pressure 0.001-0.003MPa to set temperature, and repetition carries
Take 2 ~ 4 times, 2 ~ 4 gained are screwed out into liquid concentrates in Rotary Evaporators, and thickening temperature is 70-80 DEG C, air pressure 0.001-
0.003MPa, untill being concentrated into stiff paste form, produce the leaf of Moringa tunning.
4. Parent Female of Macrobrachium rosenbergii resisting stress described in claim 3 promotees the preparation method of breeding pellet, it is characterized in that：The pH
Acetic acid-sodium acetate is used as pH adjusting agent for the 4.0-6.0 aqueous solution；The bacillus amyloliquefaciens FFRC-S24 was in 2013
May 9 was preserved in China and establishes culture collection, and deposit number is：CCTCC NO：M2013191, specification are 10,000,000,000
CFU/g。
5. Parent Female of Macrobrachium rosenbergii resisting stress as claimed in claim 2 promotees the preparation method of breeding pellet, it is characterized in that：It is described
Pellet particle diameter is 1.0-3.0mm.
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| CN108464413A (en) * | 2018-06-28 | 2018-08-31 | 中国水产科学研究院淡水渔业研究中心 | A kind of pond industrialization circulating water culture system megalobrama amblycephala functional feed and preparation method thereof |
| CN108684989A (en) * | 2018-05-17 | 2018-10-23 | 广西壮族自治区水产科学研究院 | A kind of feed and preparation method thereof improving Macrobrachium rosenbergii hypoxia-resistant capacity |
| CN110506847A (en) * | 2019-09-23 | 2019-11-29 | 南京欣腾瑞生物科技有限公司 | A kind of pet nutrition replenishers and its preparation method and application |
| CN111317079A (en) * | 2020-04-24 | 2020-06-23 | 江苏富裕达粮食制品股份有限公司 | Early functional feed for macrobrachium rosenbergii and preparation method thereof |
| CN111418718A (en) * | 2020-04-24 | 2020-07-17 | 江苏富裕达粮食制品股份有限公司 | Compound feed bred from full-plant protein macrobrachium rosenbergii and preparation method of compound feed |
| CN114277061A (en) * | 2021-09-26 | 2022-04-05 | 中国水产科学研究院淡水渔业研究中心 | Preparation method of fermented moringa oleifera leaves and application of fermented moringa oleifera leaves in aquaculture |
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| CN108684989A (en) * | 2018-05-17 | 2018-10-23 | 广西壮族自治区水产科学研究院 | A kind of feed and preparation method thereof improving Macrobrachium rosenbergii hypoxia-resistant capacity |
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| CN111418718A (en) * | 2020-04-24 | 2020-07-17 | 江苏富裕达粮食制品股份有限公司 | Compound feed bred from full-plant protein macrobrachium rosenbergii and preparation method of compound feed |
| CN114277061A (en) * | 2021-09-26 | 2022-04-05 | 中国水产科学研究院淡水渔业研究中心 | Preparation method of fermented moringa oleifera leaves and application of fermented moringa oleifera leaves in aquaculture |
| CN114277061B (en) * | 2021-09-26 | 2023-10-31 | 中国水产科学研究院淡水渔业研究中心 | Preparation method of fermented moringa leaves and application of fermented moringa leaves in aquaculture |
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