CN107494543A - A kind of root-growing agent and its outside sprout-cultivating-bottle method for cherry tissue culture outside sprout-cultivating-bottle radication - Google Patents
A kind of root-growing agent and its outside sprout-cultivating-bottle method for cherry tissue culture outside sprout-cultivating-bottle radication Download PDFInfo
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- CN107494543A CN107494543A CN201710647701.8A CN201710647701A CN107494543A CN 107494543 A CN107494543 A CN 107494543A CN 201710647701 A CN201710647701 A CN 201710647701A CN 107494543 A CN107494543 A CN 107494543A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
- A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/14—Boron; Compounds thereof
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Wood Science & Technology (AREA)
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- Inorganic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
The present invention relates to a kind of root-growing agent and its outside sprout-cultivating-bottle method for cherry tissue culture outside sprout-cultivating-bottle radication, root-growing agent includes 1.0mg/mL heteroauxins (IAA) and 35.0mg/mL chlorogenic acids (CA)；Matrix of taking root in the method for outside sprout-cultivating-bottle is turf and the mixture of perlite, and the mass ratio of turf and perlite is 2.5 3.5:1, choose the root-growing agent of the present invention；Then tissue-cultured seedling is cut into single stem branch seedling, 8 13min is soaked in root-growing agent, are then inserted into matrix；Hole tray is placed in shed and cultivated 60 days.Using the root-growing agent of the present invention, cherry tissue-cultured seedling can reach 100% by outside sprout-cultivating-bottle method of the present invention, the rooting rate of tissue-cultured seedling, and the present invention substantially reduces incubation time, improves rooting rate, and add quantity of averagely taking root.
Description
Technical field
The present invention relates to a kind of root-growing agent and its outside sprout-cultivating-bottle method for cherry tissue culture outside sprout-cultivating-bottle radication, belong to cherry
Cultivating techniques field.
Background technology
Cherry belongs to rose family Prunus, is generally acknowledged tissue culture expanding propagation and bottle in field of plant tissue culture as apple
A kind of plant of outer difficulty of taking root.
The alga fertilizer of present most of root-inducing powders using in the market finished product either some growth-promoting roots.Prior art
In, cuttage branch base portion (3-5cm) 24h is soaked with 25-500mg/kg methyl α-naphthyl acetates decoction, rooting of cuttings can be promoted, raising survives
Rate；It is mainly used in rooting of cuttings, can induce the formation of root substance, promote cell differentiation and division, is advantageous to new root generation and dimension
The differentiation of tube bundle system, promote the formation of cutting adventitious root.However, these finished product root-growing agents typically have it is relatively good general
Property, but selectivity is not strong for cherry, the DeGrain of hestening rooting.
The patent application of Application No. 201610641774.1, a kind of entitled outside sprout-cultivating-bottle side of cherry tissue-cultured seedling
Method, including the clip for material of taking root, the preparation for facility of taking root, the preparation of nutrient solution, the cuttage of culturing young plants, period management of taking root,
Hardening period management, point cultivation period management；Wherein, take root in nutrient solution containing ammonium nitrate, potassium nitrate, potassium dihydrogen sulfate, magnesium sulfate, chlorine
Change calcium, KI, boric acid, manganese sulfate, zinc sulfate, sodium molybdate, copper sulphate, cobalt chloride, indolebutyric acid and heteroauxin, by this
The rooting method of invention makes cherry tissue-cultured seedling rooting rate be up to more than 90%, but the species of nutrient solution is more in the invention,
Prepare complicated.
The content of the invention
It is an object of the invention to a kind of root-growing agent and its outside sprout-cultivating-bottle method for cherry tissue culture outside sprout-cultivating-bottle radication, sheet
Invention is time-consuming short, and rooting rate is high, and quantity of averagely taking root is more.Technical scheme is as follows：
A kind of root-growing agent for cherry tissue culture outside sprout-cultivating-bottle radication, including 1.0mg/mL heteroauxins (IAA) and 35.0mg/
ML chlorogenic acids (CA)；
Further, the root-growing agent also includes 80mg/L boron.
Root-growing agent of the present invention is in use, be now with the current.
The method of cherry tissue culture outside sprout-cultivating-bottle radication, is comprised the following steps that：
(1) bottle cap for the cherry tissue-cultured seedling that grown 6 weeks is opened 1/6th, is placed in hardening 8 days in glasshouse, with
Adapt to external environment；
(2) matrix of taking root：Turf and perlite are 2.5-3.5 in mass ratio:1 ratio is well mixed, and is dispensed into 50 holes
Hole tray in, load it is even, watering moisturizing it is standby；
(3) root-growing agent：Root-growing agent 250mL of the present invention is prepared, is kept in dark place at low temperature standby；
(4) the cherry tissue-cultured seedling after hardening is removed into callus and tissue culture matrix, then it is 2.5-3.5cm's to be cut into plant height
Single stem branch seedling, the base portion of single stem branch seedling is placed in the root-growing agent of step (3) preparation, soaks 8-13min, then use tweezers
Clamp single stem branch seedling base portion to be inserted into the plug media of step (2) preparation, insertion depth 0.8-1.3cm, plant interval
For 3-4cm；
(5) hole tray is placed in shed, temperature is 25 DEG C, humidity 70%, intensity of illumination 2300-2500Lx, illumination
Time is 13h/d, and incubation time is 60 days.
Preferably, turf and perlite are 3 in mass ratio in step (2):1；The plant height of single stem branch seedling is in step (4)
3cm；The base portion of single stem branch seedling, which is placed in root-growing agent, in step (4) soaks 10min；Single stem branch seedling base portion is inserted in step (4)
The depth entered in plug media is 1.1cm, and plant is at intervals of 3.5cm；Intensity of illumination in step (5) is 2400Lx.
It is furthermore preferred that tissue-culture container seedling of the cherry tissue-cultured seedling selection for cherry rootstock " examining spy ".
The present invention has advantages below compared with prior art：
Using the root-growing agent of the present invention, for cherry tissue-cultured seedling by outside sprout-cultivating-bottle method of the present invention, the rooting rate of tissue-cultured seedling can
Reach 100%, the present invention substantially reduces incubation time, improves rooting rate, and add quantity of averagely taking root.
Embodiment
The invention will now be further described with reference to specific embodiments, advantages of the present invention and feature will be with description and
It is apparent.But embodiment is only exemplary, does not form any restrictions to the scope of the present invention.Those skilled in the art should
It should be appreciated that the details and form of technical solution of the present invention can be repaiied without departing from the spirit and scope of the invention
Change or replace, but these modifications and replacement are each fallen within protection scope of the present invention.
The selection of cherry tissue-cultured seedling is the tissue-culture container seedling of cherry rootstock " examining spy ".
A kind of root-growing agent and its outside sprout-cultivating-bottle method for cherry tissue culture outside sprout-cultivating-bottle radication of embodiment 1
Root-growing agent is by including 1.0mg/mL heteroauxins (IAA) and 35.0mg/mL chlorogenic acids (CA)；
The method of cherry tissue culture outside sprout-cultivating-bottle radication, is comprised the following steps that：
(1) bottle cap for the cherry tissue-cultured seedling that grown 6 weeks is opened 1/6th, is placed in hardening 8 days in glasshouse, with
Adapt to external environment；
(2) matrix of taking root：Turf and perlite are 3 in mass ratio:1 ratio is well mixed, and is dispensed into the hole tray in 50 holes
In, filling is even, and watering moisturizing is standby；
(3) root-growing agent：Root-growing agent 250mL of the present invention is prepared, is kept in dark place at low temperature standby；
(4) the cherry tissue-cultured seedling after hardening is removed into callus and tissue culture matrix, then is cut into single stem branch that plant height is 3cm
Seedling, the base portion of single stem branch seedling is placed in the root-growing agent of step (3) preparation, soaks 10min, then clamp single stem with tweezers
Branch seedling base portion is inserted into the plug media of step (2) preparation, and insertion depth 1.1cm, plant is at intervals of 3.5cm；
(5) hole tray is placed in shed, temperature is 25 DEG C, humidity 70%, intensity of illumination 2400Lx, and light application time is
13h/d, incubation time are 60 days.
Preferably, turf and perlite are 3 in mass ratio in step (2):1；The plant height of single stem branch seedling is in step (4)
3cm；The base portion of single stem branch seedling, which is placed in root-growing agent, in step (4) soaks 10min；Single stem branch seedling base portion is inserted in step (4)
The depth entered in plug media is 1.1cm, and plant is at intervals of 3.5cm；Intensity of illumination in step (5) is 2400Lx.
A kind of root-growing agent and its outside sprout-cultivating-bottle method for cherry tissue culture outside sprout-cultivating-bottle radication of embodiment 2
Root-growing agent is by including 1.0mg/mL heteroauxins (IAA) and 35.0mg/mL chlorogenic acids (CA)；
The method of cherry tissue culture outside sprout-cultivating-bottle radication, is comprised the following steps that：
(1) bottle cap for the cherry tissue-cultured seedling that grown 6 weeks is opened 1/6th, is placed in hardening 8 days in glasshouse, with
Adapt to external environment；
(2) matrix of taking root：Turf and perlite are 2.6 in mass ratio:1 ratio is well mixed, and is dispensed into the cave in 50 holes
In disk, filling is even, and watering moisturizing is standby；
(3) root-growing agent：Root-growing agent 250mL of the present invention is prepared, is kept in dark place at low temperature standby；
(4) the cherry tissue-cultured seedling after hardening is removed into callus and tissue culture matrix, then is cut into single stem that plant height is 3.3cm
Branch seedling, the base portion of single stem branch seedling is placed in the root-growing agent of step (3) preparation, is soaked 12min, is then clamped list with tweezers
Stem branch seedling base portion is inserted into the plug media of step (2) preparation, and insertion depth 1.3cm, plant is at intervals of 4cm；
(5) hole tray is placed in shed, temperature is 25 DEG C, humidity 70%, intensity of illumination 2500Lx, and light application time is
13h/d, incubation time are 60 days.
A kind of root-growing agent and its outside sprout-cultivating-bottle method for cherry tissue culture outside sprout-cultivating-bottle radication of embodiment 3
Root-growing agent is by including 1.0mg/mL heteroauxins (IAA) and 35.0mg/mL chlorogenic acids (CA)；
The method of cherry tissue culture outside sprout-cultivating-bottle radication, is comprised the following steps that：
(1) bottle cap for the cherry tissue-cultured seedling that grown 6 weeks is opened 1/6th, is placed in hardening 8 days in glasshouse, with
Adapt to external environment；
(2) matrix of taking root：Turf and perlite are 3.5 in mass ratio:1 ratio is well mixed, and is dispensed into the cave in 50 holes
In disk, filling is even, and watering moisturizing is standby；
(3) root-growing agent：Root-growing agent 250mL of the present invention is prepared, is kept in dark place at low temperature standby；
(4) the cherry tissue-cultured seedling after hardening is removed into callus and tissue culture matrix, then is cut into single stem that plant height is 2.5cm
Branch seedling, the base portion of single stem branch seedling is placed in the root-growing agent of step (3) preparation, is soaked 9min, is then clamped list with tweezers
Stem branch seedling base portion is inserted into the plug media of step (2) preparation, and insertion depth 0.9cm, plant is at intervals of 3.2cm；
(5) hole tray is placed in shed, temperature is 25 DEG C, humidity 70%, intensity of illumination 2300Lx, and light application time is
13h/d, incubation time are 60 days.
A kind of root-growing agent for cherry tissue culture outside sprout-cultivating-bottle radication of embodiment 4, including 1.0mg/mL heteroauxins (IAA),
The boron of 35.0mg/mL chlorogenic acids (CA) and 80mg/L；
The method of cherry tissue culture outside sprout-cultivating-bottle radication is the same as embodiment 1.
Comparative example 1
Root-growing agent：The special root-growing agent of cuttage and seedling culture, manufacturer are the Lu Jiu bio tech ltd of Zhengzhou three；
Outside sprout-cultivating-bottle method is the same as embodiment 1.
Comparative example 2
Root-growing agent and outside sprout-cultivating-bottle method use the patent application of Application No. 201610641774.1, and entitled one
The outside sprout-cultivating-bottle method of kind cherry tissue-cultured seedling.
Comparative example 3
Root-growing agent uses the patent application of Application No. 201610641774.1, a kind of entitled cherry tissue-cultured seedling
Nutrient solution in outside sprout-cultivating-bottle method；Rooting method is the same as embodiment 2.
Comparative example 4
Root-growing agent：Clear water
Outside sprout-cultivating-bottle method is the same as embodiment 1.
The test example present invention compareing in cherry rooting rate and quantity of averagely taking root with comparative example 1-4
Detection time is 10 days, 20 days, 30 days, 40 days, 50 days and 60 days that tissue-cultured seedling is inserted after matrix.As a result such as table 1
Shown in table 2.
The rooting rate of the present invention of table 1 and cherry in comparative example 1-4 in different time points
As shown in Table 1, cherry tissue culture outside sprout-cultivating-bottle radication is carried out using the present invention, rooting rate is implemented more than 95%
Rooting rate in example has reached 100%.At the 60th day, rooting rate was respectively 90% and 91% for comparative example 2 and comparative example 3, but
It is not less than 95%.
The quantity of taking root that is averaged of the present invention of table 2 and cherry in comparative example 1-4 in different time points
As shown in Table 2, cherry tissue culture outside sprout-cultivating-bottle radication is carried out using the present invention, at the 60th day, quantity of averagely taking root is 6.0
It is more than bar；During comparative example 1 the 60th day, quantity of averagely taking root is 3.2, and during comparative example 2 the 60th day, quantity of averagely taking root is 4.3
Bar, during comparative example 3 the 60th day, quantity of averagely taking root is 4.3, and during comparative example 4 the 60th day, quantity of averagely taking root is only 3.0.
Above-mentioned result of the test shows, cherry tissue culture outside sprout-cultivating-bottle radication agent of the invention and rooting method, either rooting rate
Still take root quantity, hence it is evident that better than other root-growing agents and rooting method.
Claims (8)
1. a kind of root-growing agent for cherry tissue culture outside sprout-cultivating-bottle radication, it is characterised in that root-growing agent includes 1.0mg/mL indoles second
Sour (IAA) and 35.0mg/mL chlorogenic acids (CA).
2. root-growing agent according to claim 1, it is characterised in that the root-growing agent also includes 80mg/L boron.
3. a kind of method of cherry tissue culture outside sprout-cultivating-bottle radication, is comprised the following steps that：
(1) bottle cap for the cherry tissue-cultured seedling that grown 6 weeks is opened 1/6th, hardening 8 days in glasshouse is placed in, to adapt to
External environment；
(2) matrix of taking root：Turf and perlite are 2.5-3.5 in mass ratio:1 ratio is well mixed, and is dispensed into the cave in 50 holes
In disk, filling is even, and watering moisturizing is standby；
(3) root-growing agent：Root-growing agent 250mL as claimed in claim 1 or 2 is prepared, is kept in dark place at low temperature standby；
(4) the cherry tissue-cultured seedling after hardening is removed into callus and tissue culture matrix, then is cut into single stem that plant height is 2.5-3.5cm
Branch seedling, the base portion of single stem branch seedling is placed in the root-growing agent of step (3) preparation, is soaked 8-13min, is then clamped with tweezers
Single stem branch seedling base portion is inserted into the plug media of step (2) preparation, and insertion depth 0.8-1.3cm, plant is at intervals of 3-
4cm；
(5) hole tray is placed in shed, temperature is 25 DEG C, humidity 70%, intensity of illumination 2300-2500Lx, light application time
For 13h/d, incubation time is 60 days.
4. according to the method for claim 3, it is characterised in that turf and perlite are in mass ratio in the step (2)
3:1。
5. according to the method for claim 3, it is characterised in that the plant height of single stem branch seedling is 3cm in the step (4).
6. according to the method for claim 3, it is characterised in that the base portion of single stem branch seedling is placed in life in the step (4)
10min is soaked in root agent.
7. according to the method for claim 3, it is characterised in that single stem branch seedling base portion insertion hole tray in the step (4)
Depth in matrix is 1.1cm, and plant is at intervals of 3.5cm.
8. according to the method for claim 3, it is characterised in that the intensity of illumination in step (5) is 2400Lx.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710647701.8A CN107494543A (en) | 2017-08-01 | 2017-08-01 | A kind of root-growing agent and its outside sprout-cultivating-bottle method for cherry tissue culture outside sprout-cultivating-bottle radication |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710647701.8A CN107494543A (en) | 2017-08-01 | 2017-08-01 | A kind of root-growing agent and its outside sprout-cultivating-bottle method for cherry tissue culture outside sprout-cultivating-bottle radication |
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| Publication Number | Publication Date |
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| CN107494543A true CN107494543A (en) | 2017-12-22 |
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| CN201710647701.8A Pending CN107494543A (en) | 2017-08-01 | 2017-08-01 | A kind of root-growing agent and its outside sprout-cultivating-bottle method for cherry tissue culture outside sprout-cultivating-bottle radication |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108243756A (en) * | 2018-03-20 | 2018-07-06 | 山东农业大学 | A kind of Tissue-cultured apple seedling bottle grafts the micro-grafting method taken root outside |
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| CN102138486A (en) * | 2011-04-01 | 2011-08-03 | 天津樱桃谷农业科技发展有限公司 | Method for carrying out rapid cuttage propagation on plantlets of prunus pseudocerasus |
| CN103155867A (en) * | 2011-12-10 | 2013-06-19 | 天水市果树研究所 | Large cherry rootstock G-7 rapid propagation method |
| CN105638479A (en) * | 2016-02-03 | 2016-06-08 | 山东省果树研究所 | Method for rapidly propagating large cherries |
| CN106258958A (en) * | 2016-08-08 | 2017-01-04 | 天水市果树研究所 | A kind of outside sprout-cultivating-bottle method of Fructus Pruni pseudocerasi tissue cultured seedling |
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2017
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102138486A (en) * | 2011-04-01 | 2011-08-03 | 天津樱桃谷农业科技发展有限公司 | Method for carrying out rapid cuttage propagation on plantlets of prunus pseudocerasus |
| CN103155867A (en) * | 2011-12-10 | 2013-06-19 | 天水市果树研究所 | Large cherry rootstock G-7 rapid propagation method |
| CN105638479A (en) * | 2016-02-03 | 2016-06-08 | 山东省果树研究所 | Method for rapidly propagating large cherries |
| CN106258958A (en) * | 2016-08-08 | 2017-01-04 | 天水市果树研究所 | A kind of outside sprout-cultivating-bottle method of Fructus Pruni pseudocerasi tissue cultured seedling |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108243756A (en) * | 2018-03-20 | 2018-07-06 | 山东农业大学 | A kind of Tissue-cultured apple seedling bottle grafts the micro-grafting method taken root outside |
| CN108243756B (en) * | 2018-03-20 | 2020-04-14 | 山东农业大学 | Micro-grafting method for grafting and rooting outside apple tissue culture seedling bottle |
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