CN107318647A - A kind of root-growing agent and its outside sprout-cultivating-bottle method for blueberry tissue culture outside sprout-cultivating-bottle radication - Google Patents
A kind of root-growing agent and its outside sprout-cultivating-bottle method for blueberry tissue culture outside sprout-cultivating-bottle radication Download PDFInfo
- Publication number
- CN107318647A CN107318647A CN201710418664.3A CN201710418664A CN107318647A CN 107318647 A CN107318647 A CN 107318647A CN 201710418664 A CN201710418664 A CN 201710418664A CN 107318647 A CN107318647 A CN 107318647A
- Authority
- CN
- China
- Prior art keywords
- root
- growing agent
- cultivating
- bottle
- tissue culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
The present invention relates to a kind of root-growing agent and its outside sprout-cultivating-bottle method for blueberry tissue culture outside sprout-cultivating-bottle radication, root-growing agent includes 1.5mg/mL indolebutyric acids (IBA) and the methyl α-naphthyl acetates of 0.4mg/mL 1 (NAA).Matrix of taking root in the method for outside sprout-cultivating-bottle is turf and the mixture of perlite, and the mass ratio of turf and perlite is 2.5 3.5:1, choose the root-growing agent of the present invention；Then tissue-cultured seedling is cut into single stem branch seedling, 8 15s is soaked in root-growing agent, matrix is then inserted into；Hole tray is placed in shed and cultivated 60 days.The present invention substantially reduced the outside sprout-cultivating-bottle time of tissue-cultured seedling, improves rooting rate, by the incubation time of more than 60 days, and the rooting rate of tissue-cultured seedling can reach 100%.
Description
Technical field
The present invention relates to a kind of root-growing agent and outside sprout-cultivating-bottle method for blueberry tissue culture outside sprout-cultivating-bottle radication, belong to blueberry training
Educate technical field.
Background technology
Blueberry can prevent uterine cancer as nutritious fruit, its folic acid contained, and the ursolic acid contained is to maincenter god
Through there is obvious stable and cooling effect, the normal body temperature of mouse can be substantially reduced, to external gram-positive bacterium, feminine gender
Bacterium and saccharomycete have inhibitory activity etc..
The young tender stem branch of blueberry tissue culture seedling has very strong meristematic capacity, and growth is fast.But the ability for being divided into root compares
Weak, outside sprout-cultivating-bottle is relatively difficult.The sea of present most of root-inducing powders for using in the market finished product either some growth-promoting roots
Algae fertilizer.In the prior art, cuttage branch base portion (3-5cm) 24h is soaked with 25-500mg/kg methyl α-naphthyl acetates decoction, cutting can be promoted
Take root, improve survival rate；It is mainly used in rooting of cuttings, can induce the formation of root substance, promotes cell differentiation and division, be conducive to
New root generates the differentiation with fibrovascular system, promotes the formation of cutting adventitious root.However, these finished product root-growing agents typically have than
Preferable versatility, but the selectivity to blueberry is not strong, takes root slow, rooting rate is low.By taking blueberry kind Lai Gexi as an example, market
Finished product root-growing agent only had 66% in the rooting rate of 60 days.
Application No. 201410054258.X patent application, a kind of entitled blueberry tissue culture outside sprout-cultivating-bottle radication side
Method, discloses blueberry tissue culture seedling blueberry seedling in the incubation time of seeding room is+2-3 days 5-7 days, the 5th step and cultivates 1-2 weeks
Recession canopy, continues to obtain the blueberry liver moss seedling of stalwartness after cultivating 4 months, root-growing agent is preferably 1g/L IBA, and whole process takes
More than 4 months, final rooting rate highest only reached 85%, it can be seen that, the technology is on rootage duration or long,
And rooting rate is also less than 90%.
The content of the invention
For the problems of the prior art, the invention provides a kind of root-growing agent for blueberry tissue culture outside sprout-cultivating-bottle radication and
Outside sprout-cultivating-bottle method, the present invention is time-consuming short, and rooting rate is high.Technical scheme is as follows：
A kind of root-growing agent for blueberry tissue culture outside sprout-cultivating-bottle radication, including 1.5mg/mL indolebutyric acids (IBA) and 0.4mg/
ML 1- methyl α-naphthyl acetates (NAA)；
Further, the root-growing agent also includes 200mg/L boron.
The method of blueberry tissue culture outside sprout-cultivating-bottle radication, is comprised the following steps that：
(1) bottle cap for the blueberry tissue culture seedling that grown eight weeks is opened into a quarter, is placed in hardening 5 days in glasshouse,
To adapt to external environment；
(2) matrix of taking root：Turf and perlite are 2.5-3.5 in mass ratio:1 ratio is well mixed, and is dispensed into 50 holes
Hole tray in, load it is even, moisturizing of watering is standby；
(3) root-growing agent：Root-growing agent 250mL of the present invention is prepared, is kept in dark place at low temperature standby；
(4) the blueberry tissue culture seedling after hardening is removed into callus and tissue culture matrix, then it is 2.5-4cm's to be cut into plant height
Single stem branch seedling, the base portion of single stem branch seedling is placed in the root-growing agent of step (3) preparation, 8-15s is soaked, is then pressed from both sides with tweezers
Firmly single stem branch seedling base portion is inserted into the plug media of step (2) preparation, and insertion depth is 1-1.5cm, and plant is at intervals of 3-
4cm；
(5) hole tray is placed in shed, temperature is 25 DEG C, and humidity is that 70%, intensity of illumination is 2300-2500Lx, illumination
Time is 13h/d, and incubation time is 60 days.
It is preferred that, turf and perlite are 3 in mass ratio in step (2):1；The plant height of single stem branch seedling is in step (4)
3cm；The base portion of single stem branch seedling, which is placed in root-growing agent, in step (4) soaks 10s；Single stem branch seedling base portion insertion in step (4)
Depth in plug media is 1.2cm, and plant is at intervals of 3.5cm；Intensity of illumination in step (5) is 2400Lx.
It is furthermore preferred that the kind of blueberry is Lai Gexi.
The present invention has advantages below compared with prior art：
Using the root-growing agent of the present invention, blueberry tissue culture seedling was by outside sprout-cultivating-bottle method of the present invention, by the culture of more than 60 days
Time, the rooting rate of tissue-cultured seedling can reach 100%, and the present invention substantially reduces incubation time, and improves rooting rate.
Embodiment
The invention will now be further described with reference to specific embodiments, advantages of the present invention and feature will be with description and
It is apparent.But embodiment is only exemplary, does not constitute any limitation to the scope of the present invention.Those skilled in the art should
It should be appreciated that, the details and form of technical solution of the present invention can be repaiied without departing from the spirit and scope of the invention
Change or replace, but these modifications and replacement are each fallen within protection scope of the present invention.
A kind of root-growing agent and its outside sprout-cultivating-bottle method for blueberry tissue culture outside sprout-cultivating-bottle radication of embodiment 1
The kind of blueberry is Lai Gexi；
Root-growing agent is made up of 1.5mg/mL indolebutyric acids (IBA) and 0.4mg/mL 1- methyl α-naphthyl acetates (NAA)；
The method of blueberry tissue culture outside sprout-cultivating-bottle radication, is comprised the following steps that：
(1) bottle cap for the blueberry tissue culture seedling that grown eight weeks is opened into a quarter, is placed in hardening 5 days in glasshouse,
To adapt to external environment；
(2) matrix of taking root：Turf and perlite are 3 in mass ratio:1 ratio is well mixed, and is dispensed into the hole tray in 50 holes
In, filling is even, and moisturizing of watering is standby；
(3) root-growing agent：Root-growing agent 250mL of the present invention is prepared, is kept in dark place at low temperature standby；
(4) the blueberry tissue culture seedling after hardening is removed into callus and tissue culture matrix, then is cut into single stem that plant height is 3cm
Branch seedling, the base portion of single stem branch seedling is placed in the root-growing agent of step (3) preparation, 10s is soaked, single stem is then clamped with tweezers
Branch seedling base portion is inserted into the plug media of step (2) preparation, and insertion depth is 1.2cm, and plant is at intervals of 3.5cm；
(5) hole tray is placed in shed, temperature is 25 DEG C, and humidity is that 70%, intensity of illumination is 2400Lx, and light application time is
13h/d, incubation time is 60 days.
Embodiment 2
A kind of root-growing agent and its outside sprout-cultivating-bottle method for blueberry tissue culture outside sprout-cultivating-bottle radication
The kind of blueberry is Lai Gexi；
Root-growing agent is made up of 1.5mg/mL indolebutyric acids (IBA) and 0.4mg/mL 1- methyl α-naphthyl acetates (NAA)；
The method of blueberry tissue culture outside sprout-cultivating-bottle radication, is comprised the following steps that：
(1) bottle cap for the blueberry tissue culture seedling that grown eight weeks is opened into a quarter, is placed in hardening 5 days in glasshouse,
To adapt to external environment；
(2) matrix of taking root：Turf and perlite are 3 in mass ratio:1 ratio is well mixed, and is dispensed into the hole tray in 50 holes
In, filling is even, and moisturizing of watering is standby；
(3) root-growing agent：Root-growing agent 250mL of the present invention is prepared, is kept in dark place at low temperature standby；
(4) the blueberry tissue culture seedling after hardening is removed into callus and tissue culture matrix, then is cut into single stem that plant height is 2.5cm
Branch seedling, the base portion of single stem branch seedling is placed in the root-growing agent of step (3) preparation, 8s is soaked, single stem is then clamped with tweezers
Branch seedling base portion is inserted into the plug media of step (2) preparation, and insertion depth is 1cm, and plant is at intervals of 3cm；
(5) hole tray is placed in shed, temperature is 25 DEG C, and humidity is that 70%, intensity of illumination is 2300Lx, and light application time is
13h/d, incubation time is 60 days.
A kind of root-growing agent and its outside sprout-cultivating-bottle method for blueberry tissue culture outside sprout-cultivating-bottle radication of embodiment 3
The kind of blueberry is Lai Gexi；
Root-growing agent is made up of 1.5mg/mL indolebutyric acids (IBA) and 0.4mg/mL 1- methyl α-naphthyl acetates (NAA)；
The method of blueberry tissue culture outside sprout-cultivating-bottle radication, is comprised the following steps that：
(1) bottle cap for the blueberry tissue culture seedling that grown eight weeks is opened into a quarter, is placed in hardening 5 days in glasshouse,
To adapt to external environment；
(2) matrix of taking root：Turf and perlite are 3 in mass ratio:1 ratio is well mixed, and is dispensed into the hole tray in 50 holes
In, filling is even, and moisturizing of watering is standby；
(3) root-growing agent：Root-growing agent 250mL of the present invention is prepared, is kept in dark place at low temperature standby；
(4) the blueberry tissue culture seedling after hardening is removed into callus and tissue culture matrix, then is cut into single stem that plant height is 4cm
Branch seedling, the base portion of single stem branch seedling is placed in the root-growing agent of step (3) preparation, 13s is soaked, single stem is then clamped with tweezers
Branch seedling base portion is inserted into the plug media of step (2) preparation, and insertion depth is 1.5cm, and plant is at intervals of 4cm；
(5) hole tray is placed in shed, temperature is 25 DEG C, and humidity is that 70%, intensity of illumination is 2500Lx, and light application time is
13h/d, incubation time is 60 days.
A kind of root-growing agent and its outside sprout-cultivating-bottle method for blueberry tissue culture outside sprout-cultivating-bottle radication of embodiment 4
Blueberry kind is Lai Gexi
Root-growing agent by 1.5mg/mL indolebutyric acids (IBA), 0.4mg/mL 1- methyl α-naphthyl acetates (NAA) and 200mg/L boron group
Into.
The method be the same as Example 1 of blueberry tissue culture outside sprout-cultivating-bottle radication.
A kind of root-growing agent for blueberry tissue culture outside sprout-cultivating-bottle radication of embodiment 5
Blueberry kind is Vaccinium ashei
Root-growing agent is made up of 1.5mg/mL indolebutyric acids (IBA) and 0.4mg/mL 1- methyl α-naphthyl acetates (NAA)；
The method be the same as Example 1 of blueberry tissue culture outside sprout-cultivating-bottle radication.
A kind of root-growing agent for blueberry tissue culture outside sprout-cultivating-bottle radication of embodiment 6
Blueberry kind is Vaccinium ashei
Root-growing agent by 1.5mg/mL indolebutyric acids (IBA), 0.4mg/mL 1- methyl α-naphthyl acetates (NAA) and 200mg/L boron group
Into.
The method be the same as Example 1 of blueberry tissue culture outside sprout-cultivating-bottle radication.
Comparative example 1
Blueberry kind：Lai Gexi
Root-growing agent：The special root-growing agent of cuttage and seedling culture, manufacturer is the Lu Jiu bio tech ltd of Zhengzhou three；
Outside sprout-cultivating-bottle method be the same as Example 1.
Comparative example 2
Blueberry kind：Lai Gexi
Root-growing agent and outside sprout-cultivating-bottle method use Application No. 201210083508.3, a kind of entitled suitable blueberry
The method of tissue-cultured seedling Rapid Rooting.
Comparative example 3
Blueberry kind：Lai Gexi
Root-growing agent：Clear water
Outside sprout-cultivating-bottle method be the same as Example 1.
The test example present invention compareing in blueberry rooting rate and quantity of averagely taking root with comparative example 1-3
Detection time is 10 days, 20 days, 30 days, 40 days, 50 days and 60 days that tissue-cultured seedling is inserted after matrix.As a result such as table 1
With shown in table 2.
The rooting rate of the present invention of table 1 and blueberry in comparative example 1-3 in different time points
As shown in Table 1, blueberry tissue culture outside sprout-cultivating-bottle radication is carried out using the present invention, rooting rate is taken root more than 90%
In agent during boron containing 200mg/L, rooting rate can reach 100%.(root-growing agent and outside sprout-cultivating-bottle method are using application for comparative example 2
Number be 201210083508.3, a kind of entitled method of suitable rapid rooting of blueberry tissue culture seedlings) although in the 10th day, the
Rooting rate is slightly above the present invention at 20 days, but its later stage rooting rate is not good, and at 60 days, rooting rate is only 83%.Meanwhile, table 1
It has been shown that, also with root-growing agent of the present invention and outside sprout-cultivating-bottle method, for the different cultivars of blueberry, on rooting rate slightly not
Together, better off of the invention of being taken root to Lai Gexi blueberries.
The quantity of taking root that is averaged of the present invention of table 2 and blueberry in comparative example 1-3 in different time points
| Sample | 10 days | 20 days | 30 days | 40 days | 50 days | 60 days |
| Embodiment 1 | 0 | 2.2 bar | 4.1 bar | 4.9 bar | 5.1 bar | 5.9 bar |
| Embodiment 2 | 0 | 2.1 bar | 3.9 bar | 4.5 bar | 4.9 bar | 5.6 bar |
| Embodiment 3 | 0 | 2.3 bar | 3.9 bar | 4.7 bar | 5.0 bar | 5.5 bar |
| Embodiment 4 | 0 | 2.6 bar | 4.3 bar | 5.3 bar | 5.5 bar | 6.2 bar |
| Embodiment 5 | 0 | 1.9 bar | 3.8 bar | 4.6 bar | 4.8 bar | 5.1 bar |
| Embodiment 6 | 0 | 2.0 bar | 3.5 bar | 4.7 bar | 5.0 bar | 5.2 bar |
| Comparative example 1 | 0 | 1.5 bar | 2.5 bar | 3.8 bar | 4.0 bar | 4.2 bar |
| Comparative example 2 | 0 | 2.1 bar | 3.5 bar | 3.9 bar | 4.1 bar | 4.5 bar |
| Comparative example 3 | 0 | 0 | 1.5 bar | 2.9 bar | 3.3 bar | 3.6 bar |
As shown in Table 2, blueberry tissue culture outside sprout-cultivating-bottle radication is carried out using the present invention, at the 60th day, quantity of averagely taking root is 5.1
More than bar, in root-growing agent during boron containing 200mg/L, rooting rate quantity of averagely taking root can reach 6.2.Comparative example 1 the 60th day
When, quantity of averagely taking root is 4.2, and (root-growing agent and outside sprout-cultivating-bottle method use Application No. to comparative example 2
201210083508.3, a kind of entitled method of suitable rapid rooting of blueberry tissue culture seedlings) the 60th day when, number of averagely taking root
Measure as 4.5.It is identical with rooting rate, also with root-growing agent of the present invention and outside sprout-cultivating-bottle method, for the different cultivars of blueberry,
It is slightly different in quantity taking root, better off of the invention of being taken root to Lai Gexi blueberries.
Claims (10)
1. a kind of root-growing agent for blueberry tissue culture outside sprout-cultivating-bottle radication, it is characterised in that the root-growing agent includes 1.5mg/mL Yin
Diindyl butyric acid and 0.4mg/mL 1- methyl α-naphthyl acetates.
2. root-growing agent according to claim 1, it is characterised in that the root-growing agent also includes 200mg/L boron.
3. a kind of method of blueberry tissue culture outside sprout-cultivating-bottle radication, is comprised the following steps that：
(1) bottle cap for the blueberry tissue culture seedling that grown eight weeks is opened into a quarter, is placed in hardening 5 days in glasshouse, with suitable
Answer external environment；
(2) matrix of taking root：Turf and perlite are 2.5-3.5 in mass ratio:1 ratio is well mixed, and is dispensed into the cave in 50 holes
In disk, filling is even, and moisturizing of watering is standby；
(3) root-growing agent：Root-growing agent 250mL as claimed in claim 1 or 2 is prepared, is kept in dark place at low temperature standby；
(4) the blueberry tissue culture seedling after hardening is removed into callus and tissue culture matrix, then is cut into single stem branch that plant height is 2.5-4cm
Seedling, the base portion of single stem branch seedling is placed in the root-growing agent of step (3) preparation, 8-15s is soaked, single stem is then clamped with tweezers
Branch seedling base portion is inserted into the plug media of step (2) preparation, and insertion depth is 1-1.5cm, and plant is at intervals of 3-4cm；
(5) hole tray is placed in shed, temperature is 25 DEG C, and humidity is that 70%, intensity of illumination is 2300-2500Lx, light application time
For 13h/d, incubation time is 60 days.
4. method according to claim 3, it is characterised in that turf and perlite are in mass ratio in the step (2)
3:1。
5. method according to claim 3, it is characterised in that the plant height of single stem branch seedling is 3cm in the step (4).
6. method according to claim 3, it is characterised in that the base portion of single stem branch seedling is placed in life in the step (4)
10s is soaked in root agent.
7. method according to claim 3, it is characterised in that single stem branch seedling base portion insertion hole tray in the step (4)
Depth in matrix is 1.2cm, and plant is at intervals of 3.5cm.
8. method according to claim 3, it is characterised in that the intensity of illumination in step (5) is 2400Lx.
9. root-growing agent according to claim 1 or 2, it is characterised in that the kind of the blueberry is Lai Gexi.
10. method according to claim 3, it is characterised in that the kind of the blueberry is Lai Gexi.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710418664.3A CN107318647A (en) | 2017-06-06 | 2017-06-06 | A kind of root-growing agent and its outside sprout-cultivating-bottle method for blueberry tissue culture outside sprout-cultivating-bottle radication |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710418664.3A CN107318647A (en) | 2017-06-06 | 2017-06-06 | A kind of root-growing agent and its outside sprout-cultivating-bottle method for blueberry tissue culture outside sprout-cultivating-bottle radication |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107318647A true CN107318647A (en) | 2017-11-07 |
Family
ID=60194038
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710418664.3A Pending CN107318647A (en) | 2017-06-06 | 2017-06-06 | A kind of root-growing agent and its outside sprout-cultivating-bottle method for blueberry tissue culture outside sprout-cultivating-bottle radication |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107318647A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108668659A (en) * | 2018-07-18 | 2018-10-19 | 苏州枫彩生态科技集团有限公司 | A kind of method of the outer cuttage root-taking of blueberry bottle |
| CN110741931A (en) * | 2019-10-15 | 2020-02-04 | 贵州省生物研究所 | Preparation method of culture medium for promoting tissue culture and rooting of blueberries |
| CN112021181A (en) * | 2020-10-11 | 2020-12-04 | 江苏东郁植物科技有限公司 | Rooting medium for blueberry tissue culture seedlings and rooting method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101416591A (en) * | 2008-12-08 | 2009-04-29 | 山东省果树研究所 | High-irrigation blueberry field young-shoot rooting plug-seeding direct seedling method |
| CN102187813A (en) * | 2011-03-31 | 2011-09-21 | 中国农业大学 | Blueberry tissue culture method and special culture medium thereof |
| CN104350912A (en) * | 2014-10-23 | 2015-02-18 | 湖南省核农学与航天育种研究所 | Cutting propagation method for southern blueberries |
| CN104430359A (en) * | 2014-10-23 | 2015-03-25 | 湖南省核农学与航天育种研究所 | Composite rooting powder as well as preparation method and application thereof |
| CN105284388A (en) * | 2015-11-18 | 2016-02-03 | 四川农业大学 | Blueberry softwood cutting seedling method |
| CN106613859A (en) * | 2017-02-08 | 2017-05-10 | 合肥师范学院 | Cultivation method of sound seedlings of blueberry tissue culture seedlings |
-
2017
- 2017-06-06 CN CN201710418664.3A patent/CN107318647A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101416591A (en) * | 2008-12-08 | 2009-04-29 | 山东省果树研究所 | High-irrigation blueberry field young-shoot rooting plug-seeding direct seedling method |
| CN102187813A (en) * | 2011-03-31 | 2011-09-21 | 中国农业大学 | Blueberry tissue culture method and special culture medium thereof |
| CN104350912A (en) * | 2014-10-23 | 2015-02-18 | 湖南省核农学与航天育种研究所 | Cutting propagation method for southern blueberries |
| CN104430359A (en) * | 2014-10-23 | 2015-03-25 | 湖南省核农学与航天育种研究所 | Composite rooting powder as well as preparation method and application thereof |
| CN105284388A (en) * | 2015-11-18 | 2016-02-03 | 四川农业大学 | Blueberry softwood cutting seedling method |
| CN106613859A (en) * | 2017-02-08 | 2017-05-10 | 合肥师范学院 | Cultivation method of sound seedlings of blueberry tissue culture seedlings |
Non-Patent Citations (2)
| Title |
|---|
| 朱世银等: ""不同蓝莓组培苗在不同基质中的瓶外生根技术初探"", 《南方农业》 * |
| 闫金玲等: ""不同处理方式对蓝莓绿枝扦插的影响"", 《安徽农业科学》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108668659A (en) * | 2018-07-18 | 2018-10-19 | 苏州枫彩生态科技集团有限公司 | A kind of method of the outer cuttage root-taking of blueberry bottle |
| CN110741931A (en) * | 2019-10-15 | 2020-02-04 | 贵州省生物研究所 | Preparation method of culture medium for promoting tissue culture and rooting of blueberries |
| CN112021181A (en) * | 2020-10-11 | 2020-12-04 | 江苏东郁植物科技有限公司 | Rooting medium for blueberry tissue culture seedlings and rooting method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105340747A (en) | Asexual rapid propagation method for radix glycyrrhizae | |
| CN105638477A (en) | Rapid propagation method for dendrobium hancockii seeds | |
| CN104186263B (en) | A kind of Cranberry cuttage seeding individual plant planting culture method | |
| CN106905034A (en) | Improve the fertilizer and method of Tomato Grafting shoot survival percent | |
| CN111034479A (en) | Cutting seedling raising method for pyrus betulaefolia | |
| CN101622955B (en) | Culture medium composition suitable for germ-free germination of orchid seeds and method thereof | |
| CN107318647A (en) | A kind of root-growing agent and its outside sprout-cultivating-bottle method for blueberry tissue culture outside sprout-cultivating-bottle radication | |
| CN101810144A (en) | Rapid breeding method of senecio cruentus | |
| CN108157187A (en) | A kind of Simple tissue culture method for broussonetia papyrifera | |
| CN103155869A (en) | Sweet cherry rootstock Colt tissue culture method | |
| CN101564010B (en) | Method for rapidly propagating tupelos | |
| CN101743908A (en) | Tissue culture, rapid propagation and cultivation method of grevillea banksii | |
| CN116671395A (en) | Cold resistance identification method for forage germplasm resources | |
| CN107853181B (en) | Tobacco explant anti-browning method based on activated carbon | |
| CN106305051A (en) | Leaf cutting propagation method of succulent plants of xPachyveria pachytoides | |
| CN105145369A (en) | Tissue culture rapid-propagation method for cymbidium bicolor | |
| CN113142060B (en) | Method for rapid and efficient propagation of Japanese beautyberry | |
| CN114885689A (en) | Cultivation method of peony seedlings | |
| CN114424748A (en) | Rooting culture and cultivation method of agar-free sweet potato virus-free test-tube plantlet | |
| CN110612903B (en) | Method for improving tissue culture secondary rooting of blueberry | |
| CN106577232A (en) | Natural propagation method for sowing seeds of dendrobium flexicaule on palisades | |
| CN111990131A (en) | Seedling raising device and seedling raising process | |
| KR101219201B1 (en) | Method for water culturing of ice plant | |
| CN105123522A (en) | Tissue culture rapid propagation method of Guangxi Mayten | |
| CN110278786A (en) | A kind of succulent stem plants culture method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171107 |
|
| RJ01 | Rejection of invention patent application after publication |