CN107473818A - A kind of culture medium and its application in asparagus and roxburgh anoectochilus terminal bud cultivation - Google Patents
A kind of culture medium and its application in asparagus and roxburgh anoectochilus terminal bud cultivation Download PDFInfo
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- CN107473818A CN107473818A CN201710713048.0A CN201710713048A CN107473818A CN 107473818 A CN107473818 A CN 107473818A CN 201710713048 A CN201710713048 A CN 201710713048A CN 107473818 A CN107473818 A CN 107473818A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C3/00—Fertilisers containing other salts of ammonia or ammonia itself, e.g. gas liquor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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Abstract
A kind of application the invention discloses culture medium and its in asparagus and roxburgh anoectochilus terminal bud cultivation, the culture medium, including following raw material：Rice straw minces, orange blossom, peanut cake, potassium chloride, sodium nitrate, ammonium sulfate, sodium iodate, calcium nitrate, magnesium sulfate, zinc nitrate, manganese chloride, Boratex, sodium molybdate, ferric nitrate, copper chloride, N lauroyl glutamates potassium, alkyl sulfate sodium, esters of acrylic acid conditioning agent, diethyllaminoethyl ethanol ester, 6 benzyl gland fat purines, indolebutyric acid, methyl α-naphthyl acetate and water.Using the culture medium cultivation asparagus and gold thread of the present invention, kill two birds with one stone, improve space availability ratio and economic benefit.
Description
【Technical field】
The invention belongs to technical field of cultivation, and in particular to a kind of culture medium and its asparagus and roxburgh anoectochilus terminal bud cultivation in
Using.
【Background technology】
Asparagus scientific name hair handle money bacterium, also known as hair handle small fire mushroom, structure bacterium, plain mushroom, dried mushroom, plain wild rice, jelly bacterium, golden mushroom, intelligence
Power mushroom etc., English are：" Enoki Mushroom ", Latin name are Flammulina velutiper (Fr.) Sing.." weight
Taste alias：See You Tomorrow」.Because its stem is elongated, like day lily, therefore claim asparagus, category Agaricales Bai Mo sections gold
Pin mushroom category, it is a kind of bacterium algae lichens.Asparagus has very high medicinal dietary function.
Asparagus is widely distributed in nature, and the ground such as China, Japan, Russia, Europe, North America, Australia has
Distribution., from the east of Jiangsu, the growth of asparagus is suitable for west to Xinjiang to Yunnan North gets Heilungkiang, south in China.Asparagus is free of
Chlorophyll, without photosynthesis, it is impossible to manufacture carbohydrate, but can be grown completely in dark surrounds, it is necessary to from culture
Ready-made organic substance is absorbed in base, is metatrophy type such as the degradation product of carbohydrate, protein and fat, is a kind of
Heterotrophic organism, belong to basidiomycetes.Asparagus is a kind of timber saprophytic bacteria, is easily grown in the withered tree of the broad leaf trees such as willow, elm, white poplar
On dry and stub.
The edible mushroom that asparagus is the autumn and winter with early spring cultivation, with its cap slide tender, handle is crisp, nutritious, tastes delicious and
It is world-famous for, particularly the first-class food materials of cold vegetable dish in sauce and chafing dish, its is nutritious, and A sweety scent assails the nostrils and delicious flavour, deep by big
Many likes.According to surveying and determination, the content of asparagus amino acid is very abundant, special higher than the content of general mushroom class, especially lysine
Not high, lysine has the function of promoting children's intelligence development；Asparagus, it can not only prevent and treat hepatopathy and stomach, intestines
Road ulcer, and also be adapted for hyperpietic, overweight people and the elderly and eat；Asparagus can suppress blood fat rise, reduce courage
Sterol, prevent and treat cardiovascular and cerebrovascular disease.Asparagus nutritional ingredient very abundant, such as protein, vitamin B1、B2Deng having dampness removing
Hot, wide chest, diuresis, hemostasis, it is lower newborn the effect of, and control lack of lactation after delivery, stewing thin pork with asparagus eats, extremely active
Effect.There is research and show, a kind of contained material has good antitumaous effect in asparagus.Asparagus is both a kind of delicious food
Product, are preferable health food again, and the domestic and international market of asparagus is increasingly wide.
Roxburgh anoectochilus terminal bud is also known as Shorthairy Antenoron, invaluable, bird ginseng etc., is rare Valuable Herbal Medicine, for orchid family Anoectochilus Blume for many years
Raw herbaceous plant, China are mainly distributed on the ground such as Fujian, Taiwan, Zhejiang, Jiangxi, Guangdong and Guangxi.Roxburgh anoectochilus terminal bud with all herbal medicine,
With refrigerant annealing, the solid lung of cool blood, wound of dispelling removing toxic substances, strengthening by means of tonics and other effects, there is extensive medical value among the people, because it is controlled
Wide, special effect is treated, is considered as " god's medicine " by among the people, Taiwan is referred to as " king of Chinese medicine "；In recent years, roxburgh anoectochilus terminal bud is applied to again
The diseases such as hypertension, diabetes, hepatitis and tumour are treated, many fine qualities such as its is medicinal, eats, views and admires are increasingly subject to
The favor of people, there is wide exploitation prospect.
Gold thread Nelumbo shade plant, light saturation point is low in its natural state, and wild gold lotus is distributed in height above sea level 300-1200m
Between, the sylvan life surface layer at the dark and damp places such as relative humidity is high, grass is less, humus is abundant is often sporadicly distributed in, likes the cool humidity of temperature
And low light environment.Under natural environment, roxburgh anoectochilus terminal bud 18-25 DEG C of thermophilic of growth, temperature growth more than 28 DEG C is suppressed, less than 5 DEG C
Undergrowth even stops growing, and gradually restores growth after temperature rise；In relative air humidity 85-92% nice and cool dark and damp gas
It is preferable to wait growth.Roxburgh anoectochilus terminal bud is typical shallow root shade plant, and because its seed is small, embryo development is not complete, natural propagation ability
Lowly, natural production is extremely rare, along with artificial long-term excessively excavation, makes its wild resource increasingly exhausted, endangered, because
This, it is necessary to a large amount of artificial cultivation to meet the market needs constantly expanded.
【The content of the invention】
Answering the technical problem to be solved in the present invention is to provide a kind of culture medium and its in asparagus and roxburgh anoectochilus terminal bud cultivation
With to solve the problems, such as encountered in asparagus and roxburgh anoectochilus terminal bud cultivation.
To solve above technical problem, the present invention uses following technical scheme：
A kind of culture medium, in units of parts by weight, including following raw material：Rice straw minces 92-108 parts, orange blossom 20-40
Part, peanut cake 15-22 parts, potassium chloride 4-7 parts, sodium nitrate 3-6 parts, ammonium sulfate 3.5-6 parts, sodium iodate 0.3-0.4 parts, calcium nitrate
0.6-0.8 parts, magnesium sulfate 0.8-1 parts, zinc nitrate 0.3-0.5 parts, manganese chloride 0.3-0.4 parts, Boratex 0.4-0.6 parts, molybdic acid
Sodium 0.3-0.5 parts, ferric nitrate 0.2-0.3 parts, copper chloride 0.2-0.4 parts, N- lauroyl glutamate potassium 0.6-0.8 parts, alkyl
Sodium sulfovinate 0.3-0.5 parts, esters of acrylic acid conditioning agent 0.2-0.4 parts, diethyllaminoethyl ethanol ester 0.2-0.3 parts, 6- benzyls
Base gland fat purine 0.1-0.16 parts, indolebutyric acid 0.08-0.15 parts, methyl α-naphthyl acetate 0.07-0.1 parts and water.
Preferably, institute's amount of water makes the water content of culture medium be 55%-58%.
Preferably, the pH value of the culture medium is 5.6-6.
The present invention also provides a kind of application of culture medium in asparagus and roxburgh anoectochilus terminal bud cultivation, the asparagus and roxburgh anoectochilus terminal bud
Cultural method, comprise the following steps：
S1：The culture of Flammulina velutipes mycelium：Culture medium for golden mushroom is prepared, is in temperature after the culture medium for golden mushroom bottling
120-125 DEG C, pressure is the 3-5min that sterilized under 150-170KPa, accesses Flammulina velutipes mycelium parent species, is cultivated at 23-26 DEG C, shape
It is standby into the Flammulina velutipes mycelium；
S2：Withered tree is erect and punching：Several withered trees are erect in warmhouse booth, described withered tree seeds include mulberry tree, banyan
Set, one kind in chestnut, distance is 45-62cm between each withered tree, is then punched on the withered tree, the method for the punching
It is：The aperture beaten is 2.6-4.2cm, depth 4.8-9.2cm, and 30-80 degree punching obliquely, the spacing of adjacent holes is 8.6-
17cm, from the ground 0.4-0.6m；
S3：The transplanting of roxburgh anoectochilus terminal bud：Long 4.2-5.3cm roxburgh anoectochilus terminal bud is transplanted into described in step S2 in the hole of withered tree, simultaneously
Load Flammulina velutipes mycelium and culture medium for cultivating；
S4：Warmhouse booth takes sunshade net sunshade, control temperature, humidity；
S5：Carry out the work of the disasters such as asparagus, the diseases prevention of roxburgh anoectochilus terminal bud, insect prevention；
S6：The Flammulina velutipes mycelium can harvest when cultivating 12-17cm a length of to entity, after roxburgh anoectochilus terminal bud cultivation 1.2-2
Harvesting.
Preferably, culture medium for golden mushroom described in step S1, in units of parts by weight, including following raw material：Rice straw minces
92-108 parts, orange blossom 20-40 parts, peanut cake 15-22 parts, potassium chloride 4-7 parts, sodium nitrate 3-6 parts, ammonium sulfate 3.5-6 parts, iodine
Sour sodium 0.3-0.4 parts, calcium nitrate 0.6-0.8 parts, magnesium sulfate 0.8-1 parts, zinc nitrate 0.3-0.5 parts, manganese chloride 0.3-0.4 parts,
Boratex 0.4-0.6 parts, sodium molybdate 0.3-0.5 parts, ferric nitrate 0.2-0.3 parts, copper chloride 0.2-0.4 parts and water.
Preferably, institute's amount of water makes the water content of culture medium for golden mushroom be 60%-64%.
Preferably, the pH value of the culture medium for golden mushroom is 5.5-6.2.
Preferably, the mesh number of sunshade net described in step S4 is 50-100.
Preferably, it is 16-20 DEG C that temperature is controlled described in step S4.
Preferably, humidity described in step S4 is 83%-88%.
The effect of medium component of the present invention is as follows：
Rice straw minces, contains abundant protein, carbohydrate, mineral matter, vitamin, cellulose and half in peanut cake
Cellulose etc., it is favourable to asparagus and roxburgh anoectochilus terminal bud.
Orange blossom contains sugar, can directly be absorbed by plant, is advantageous to the accumulation of plant polyose.
The nutritive effect of potassium element can improve photosynthetic intensity, promote the formation of starch and sugar in crop body, increase
Pretend the resistance and resistance against diseases of thing, moreover it is possible to improve crop and nitrogen is absorbed.
Nitrogen is the essential element that protein is formed, and protein is the base substance in cellular plasm composition.Nitrogen energy
Promote the formation of protein and chlorophyll, make dark green leaf color, leaf area increase, promote the assimilation of carbon, be advantageous to yield increase, product
Matter improves.
Phosphorus is to form the indispensable element such as Nuclear extract, lecithin.P elements can accelerate cell division, promote root
System and overground part accelerate growth, promote bud differentiation, ahead of time maturation, improve fruit quality.
Iodine is the indispensable element of plant growth, is described as biological element, wisdom element.
It is slack-off to lack the growth of calcium plant meristem, calcium deficiency and disease are just in growing point and spire, when serious, leaf deformation
And chlorosis, there is necrotic spot at the edge of leaf.
Magnesium is the constituent of chlorophyll.If lacking magnesium, blade loses green, it is impossible to manufactures nutriment.
Zinc can promote crop to carry out photosynthesis, and it is the constituent of a variety of enzymes, and crop growth occurs during zinc-deficiency
Stagnate.
Contain manganese in plant chloroplast, manganese can promote germination and seedling period to grow, and the light of crop is influenceed during manganese deficiency
The accumulation of cooperation use, respiration, nitrate nitrogen in vivo.Chlorosis yellow between blade arteries and veins is shown as, there is brown spot.Boron is in plant
Relatively concentrate in stem apex, the tip of a root, blade and floral organ in vivo, the content in dicotyledon is usually above monocotyledon.
Boron can improve the nitrogenase activity of legume rhizobium, increase amount of nitrogen fixation, and during boron deficiency, root stays undeveloped, influences amount of nitrogen fixation.Boron
Crop anti-adversity can also be strengthened.
Molybdenum is nitrogen-fixing microorganism, is particularly fixed with the rhizobium of legume symbiosis necessary during air nitrogen.Together
When and can promote leaf photosynthesis intensity.Plant is short and small when lacking molybdenum, and chlorosis between blade arteries and veins, leaf margin is dried-up, inward curl, into withering
Listless state.
Iron is that chlorophyll formation is indispensable, is difficult transfer in plant body, so blade " chlorosis " is that plant lacks
The performance of iron, and this chlorosis is shown on young leaflet tablet first.
The photosynthesis of copper participation plant, respiration, nitrogen metabolism, the resistance for strengthening plant.Plant strain growth is thin when lacking copper
Weak, young leaves jaundice is wilting withered, and blade tip curling is turned white, and has necrotic spot.
N- lauroyl glutamates potassium can make nutritional ingredient effectively scattered, beneficial to plant absorption.
Alkyl sulfate sodium can make plant effectively absorb nutritional ingredient as a kind of bleeding agent.
Esters of acrylic acid conditioning agent can play a part of regulation, plant is fully absorbed nutritional ingredient.
Diethyllaminoethyl ethanol ester can play synergistic effect.
6-benzyladenine, Chinese nickname are 2- benayl aminopurines, N- benzyl adenosines, n-butyl acrylate, white or class
White crystal, water is insoluble in, is slightly soluble in ethanol, it is stable in acid, alkali, belong to broad spectrum activity plant growth regulator, plant can be promoted
Cell growth, suppress the degraded of plant chlorophyll, improve the content of amino acid, Delaying Leaf-Senescence etc..
Indolebutyric acid is mainly used in rooting of cuttings, can induce the formation of root substance, promotes cell differentiation and division, is advantageous to
New root generates and the differentiation of fibrovascular system, promotes the formation of cutting adventitious root.
Methyl α-naphthyl acetate (1-Naphthaleneacetic acid), abbreviation NAA, is a kind of organic compound, is a kind of readily soluble
In the colorless solid of organic solvent, its structure is substituted for No. 1 position of naphthalene with carboxymethyl, and it is Plant Hormone growth
Element, it is usually used in the root of hair powder or rooting agent of commercialization, is used when plant is using cuttage breeding.It can also be used for plant tissue
Culture.
The invention has the advantages that：
(1) method of the invention, by analyzing asparagus and roxburgh anoectochilus terminal bud growth conditions, find nutritional ingredients needed for both and
PH is similar, in addition both Xi Yin, middle low temperature, high air humidity, and therefore, asparagus and roxburgh anoectochilus terminal bud cultivation can phase alternates
It is long, so that also coproduction asparagus while cultivation obtains roxburgh anoectochilus terminal bud, kills two birds with one stone, improve space availability ratio and economic benefit；
(2) culture medium for cultivating prepared of the present invention is nutritious, collocation is reasonable, synergy is generated, with prior art
Compare, the roxburgh anoectochilus terminal bud yield that method of the invention is planted is high, quality is excellent (contains more active ingredients：Taurine, polysaccharide, ammonia
Base acid etc.)；
(3) asparagus cycle for cultivating of the present invention is short, yield is high, quality is excellent；
(4) method of the invention is green, and simple to operate, production cost is low, is easy to industrialized development.
【Embodiment】
For ease of more fully understanding the present invention, it is illustrated by following examples, these embodiments belong to the present invention's
Protection domain, but do not limit the scope of the invention.
In embodiment, the new cultivation technology of the asparagus and roxburgh anoectochilus terminal bud, comprise the following steps：
S1：The culture of Flammulina velutipes mycelium：Prepare culture medium for golden mushroom, the culture medium for golden mushroom in units of parts by weight,
Including following raw material：Rice straw minces 92-108 parts, orange blossom 20-40 parts, peanut cake 15-22 parts, potassium chloride 4-7 parts, sodium nitrate
3-6 parts, ammonium sulfate 3.5-6 parts, sodium iodate 0.3-0.4 parts, calcium nitrate 0.6-0.8 parts, magnesium sulfate 0.8-1 parts, zinc nitrate 0.3-
0.5 part, manganese chloride 0.3-0.4 parts, Boratex 0.4-0.6 parts, sodium molybdate 0.3-0.5 parts, ferric nitrate 0.2-0.3 parts, copper chloride
0.2-0.4 parts and water, institute's amount of water make the water content of culture medium be 60%-64%, and the pH value of the culture medium is 5.5-6.2,
In temperature it is 120-125 DEG C after culture medium for golden mushroom bottling, pressure is the 3-5min that sterilized under 150-170KPa, accesses acupuncture needle
Mushroom screw kind, is cultivated at 23-26 DEG C, forms the Flammulina velutipes mycelium, standby；
S2：Withered tree is erect and punching：Several withered trees are erect in warmhouse booth, described withered tree seeds include mulberry tree, banyan
Set, one kind in chestnut, distance is 45-62cm between each withered tree, is then punched on the withered tree, the method for the punching
It is：The aperture beaten is 2.6-4.2cm, depth 4.8-9.2cm, and 30-80 degree punching obliquely, the spacing of adjacent holes is 8.6-
17cm, from the ground 0.4-0.6m；
S3：The transplanting of roxburgh anoectochilus terminal bud：Long 4.2-5.3cm roxburgh anoectochilus terminal bud is transplanted into described in step S2 in the hole of withered tree, simultaneously
Load Flammulina velutipes mycelium and culture medium for cultivating, the culture medium for cultivating is in units of parts by weight, including following raw material：Rice straw minces
92-108 parts, orange blossom 20-40 parts, peanut cake 15-22 parts, potassium chloride 4-7 parts, sodium nitrate 3-6 parts, ammonium sulfate 3.5-6 parts, iodine
Sour sodium 0.3-0.4 parts, calcium nitrate 0.6-0.8 parts, magnesium sulfate 0.8-1 parts, zinc nitrate 0.3-0.5 parts, manganese chloride 0.3-0.4 parts,
Boratex 0.4-0.6 parts, sodium molybdate 0.3-0.5 parts, ferric nitrate 0.2-0.3 parts, copper chloride 0.2-0.4 parts, N- lauroyl paddy
Propylhomoserin potassium 0.6-0.8 parts, alkyl sulfate sodium 0.3-0.5 parts, esters of acrylic acid conditioning agent 0.2-0.4 parts, diethyllaminoethyl
Ethanol ester 0.2-0.3 parts, 6- benzyl gland fat purine 0.1-0.16 parts, indolebutyric acid 0.08-0.15 parts, methyl α-naphthyl acetate 0.07-0.1 parts and
Water, institute's amount of water make the water content of culture medium for cultivating be 55%-58%, and the pH value of the culture medium for cultivating is 5.6-6；
S4：Warmhouse booth takes the sunshade net sunshade of 50-100 mesh, and to control temperature be 16-20 DEG C, humidity 83%-
88%；
S5：Carry out the work of the disasters such as asparagus, the diseases prevention of roxburgh anoectochilus terminal bud, insect prevention；
S6：The Flammulina velutipes mycelium can harvest when cultivating 12-17cm a length of to entity, after roxburgh anoectochilus terminal bud cultivation 1.2-2
Harvesting.
It is illustrated below with more specifically embodiment.
Embodiment 1
A kind of new cultivation technology of asparagus and roxburgh anoectochilus terminal bud, comprises the following steps：
S1：The culture of Flammulina velutipes mycelium：Prepare culture medium for golden mushroom, the culture medium for golden mushroom in units of parts by weight,
Including following raw material：Rice straw mince 102 parts, 30 parts of orange blossom, 20 parts of peanut cake, 6 parts of potassium chloride, 5 parts of sodium nitrate, ammonium sulfate 5
Part, 0.35 part of sodium iodate, 0.7 part of calcium nitrate, 0.9 part of magnesium sulfate, 0.4 part of zinc nitrate, 0.35 part of manganese chloride, 0.5 part of Boratex,
0.4 part of sodium molybdate, 0.2 part of ferric nitrate, 0.3 part of copper chloride and water, institute's amount of water make the water content of culture medium be 63%, the training
The pH value for supporting base is 5.8, in temperature is 123 DEG C after culture medium for golden mushroom bottling, pressure is the 4min that sterilized under 160KPa, is connect
Enter Flammulina velutipes mycelium parent species, cultivated at 25 DEG C, form the Flammulina velutipes mycelium, it is standby；
S2：Withered tree is erect and punching：Several withered trees are erect in warmhouse booth, described withered tree seeds include mulberry tree, banyan
Set, one kind in chestnut, distance is 52cm between each withered tree, is then punched on the withered tree, the method for the punching is：
The aperture beaten is 3.8cm, depth 7cm, and 70 degree of punchings obliquely, the spacing of adjacent holes is 13cm, from the ground 0.5m；
S3：The transplanting of roxburgh anoectochilus terminal bud：Long 4.8cm roxburgh anoectochilus terminal bud is transplanted into described in step S2 in the hole of withered tree, loaded simultaneously
Flammulina velutipes mycelium and culture medium for cultivating, the culture medium for cultivating is in units of parts by weight, including following raw material：Rice straw minces 102
Part, 30 parts of orange blossom, 20 parts of peanut cake, 6 parts of potassium chloride, 5 parts of sodium nitrate, 5 parts of ammonium sulfate, 0.35 part of sodium iodate, calcium nitrate 0.7
Part, 0.9 part of magnesium sulfate, 0.4 part of zinc nitrate, 0.35 part of manganese chloride, 0.5 part of Boratex, 0.4 part of sodium molybdate, 0.2 part of ferric nitrate,
0.3 part of copper chloride, 0.7 part of N- lauroyl glutamates potassium, 0.4 part of alkyl sulfate sodium, 0.3 part of esters of acrylic acid conditioning agent,
0.2 part of diethyllaminoethyl ethanol ester, 0.14 part of 6- benzyl gland fats purine, 0.12 part of indolebutyric acid, 0.09 part of methyl α-naphthyl acetate and water, institute
Amount of water makes the water content of culture medium for cultivating be 56%, and the pH value of the culture medium for cultivating is 5.8；
S4：Warmhouse booth takes the sunshade net sunshade of 80 mesh, and to control temperature be 18 DEG C, humidity 86%；
S5：Carry out the work of the disasters such as asparagus, the diseases prevention of roxburgh anoectochilus terminal bud, insect prevention；
S6：The Flammulina velutipes mycelium can harvest when cultivating 15cm a length of to entity, and roxburgh anoectochilus terminal bud harvests after cultivating 1.5 years.
Embodiment 2
A kind of new cultivation technology of asparagus and roxburgh anoectochilus terminal bud, comprises the following steps：
S1：The culture of Flammulina velutipes mycelium：Prepare culture medium for golden mushroom, the culture medium for golden mushroom in units of parts by weight,
Including following raw material：Rice straw mince 93 parts, 22 parts of orange blossom, 15 parts of peanut cake, 4 parts of potassium chloride, 3 parts of sodium nitrate, ammonium sulfate 3.5
Part, 0.3 part of sodium iodate, 0.6 part of calcium nitrate, 0.8 part of magnesium sulfate, 0.3 part of zinc nitrate, 0.3 part of manganese chloride, 0.4 part of Boratex, molybdenum
Sour 0.3 part of sodium, 0.2 part of ferric nitrate, 0.2 part of copper chloride and water, institute's amount of water make the water content of culture medium be 60%, the culture
The pH value of base is 5.5, in temperature is 120 DEG C after culture medium for golden mushroom bottling, and pressure is the 5min that sterilized under 150KPa, access
Flammulina velutipes mycelium parent species, cultivated at 23 DEG C, form the Flammulina velutipes mycelium, it is standby；
S2：Withered tree is erect and punching：Several withered trees are erect in warmhouse booth, described withered tree seeds include mulberry tree, banyan
Set, one kind in chestnut, distance is 45cm between each withered tree, is then punched on the withered tree, the method for the punching is：
The aperture beaten is 2.6cm, depth 4.8cm, and 30 degree of punchings obliquely, the spacing of adjacent holes is 8.6cm, from the ground 0.4m；
S3：The transplanting of roxburgh anoectochilus terminal bud：Long 4.2cm roxburgh anoectochilus terminal bud is transplanted into described in step S2 in the hole of withered tree, loaded simultaneously
Flammulina velutipes mycelium and culture medium for cultivating, the culture medium for cultivating is in units of parts by weight, including following raw material：Rice straw minces 93
Part, 22 parts of orange blossom, 15 parts of peanut cake, 4 parts of potassium chloride, 3 parts of sodium nitrate, 3.5 parts of ammonium sulfate, 0.3 part of sodium iodate, calcium nitrate
0.6 part, 0.8 part of magnesium sulfate, 0.3 part of zinc nitrate, 0.3 part of manganese chloride, 0.4 part of Boratex, 0.3 part of sodium molybdate, ferric nitrate 0.2
Part, 0.2 part of copper chloride, 0.6 part of N- lauroyl glutamates potassium, 0.3 part of alkyl sulfate sodium, esters of acrylic acid conditioning agent 0.2
Part, 0.2 part of diethyllaminoethyl ethanol ester, 0.1 part of 6- benzyl gland fats purine, 0.08 part of indolebutyric acid, 0.07 part of methyl α-naphthyl acetate and water,
Institute's amount of water makes the water content of culture medium for cultivating be 55%, and the pH value of the culture medium for cultivating is 5.6；
S4：Warmhouse booth takes the sunshade net sunshade of 50 mesh, and to control temperature be 16 DEG C, humidity 83%；
S5：Carry out the work of the disasters such as asparagus, the diseases prevention of roxburgh anoectochilus terminal bud, insect prevention；
S6：The Flammulina velutipes mycelium can harvest when cultivating 12cm a length of to entity, and roxburgh anoectochilus terminal bud harvests after cultivating 1.2 years.
Embodiment 3
A kind of new cultivation technology of asparagus and roxburgh anoectochilus terminal bud, comprises the following steps：
S1：The culture of Flammulina velutipes mycelium：Prepare culture medium for golden mushroom, the culture medium for golden mushroom in units of parts by weight,
Including following raw material：Rice straw mince 105 parts, 40 parts of orange blossom, 22 parts of peanut cake, 7 parts of potassium chloride, 6 parts of sodium nitrate, ammonium sulfate 6
Part, 0.4 part of sodium iodate, 0.8 part of calcium nitrate, 1 part of magnesium sulfate, 0.5 part of zinc nitrate, 0.4 part of manganese chloride, 0.6 part of Boratex, molybdic acid
0.5 part of sodium, 0.3 part of ferric nitrate, 0.4 part of copper chloride and water, institute's amount of water make the water content of culture medium be 64%, the culture medium
PH value be 6.2, in temperature be 125 DEG C after culture medium for golden mushroom bottling, pressure is the 3min that sterilized under 170KPa, access gold
Pin mushroom screw kind, is cultivated at 26 DEG C, forms the Flammulina velutipes mycelium, standby；
S2：Withered tree is erect and punching：Several withered trees are erect in warmhouse booth, described withered tree seeds include mulberry tree, banyan
Set, one kind in chestnut, distance is 62cm between each withered tree, is then punched on the withered tree, the method for the punching is：
The aperture beaten is 4.2cm, depth 9.2cm, and 80 degree of punchings obliquely, the spacing of adjacent holes is 17cm, from the ground 0.6m；
S3：The transplanting of roxburgh anoectochilus terminal bud：Long 5.3cm roxburgh anoectochilus terminal bud is transplanted into described in step S2 in the hole of withered tree, loaded simultaneously
Flammulina velutipes mycelium and culture medium for cultivating, the culture medium for cultivating is in units of parts by weight, including following raw material：Rice straw minces 105
Part, 40 parts of orange blossom, 22 parts of peanut cake, 7 parts of potassium chloride, 6 parts of sodium nitrate, 6 parts of ammonium sulfate, 0.4 part of sodium iodate, calcium nitrate 0.8
Part, 1 part of magnesium sulfate, 0.5 part of zinc nitrate, 0.4 part of manganese chloride, 0.6 part of Boratex, 0.5 part of sodium molybdate, 0.3 part of ferric nitrate, chlorination
0.4 part of copper, 0.8 part of N- lauroyl glutamates potassium, 0.5 part of alkyl sulfate sodium, 0.4 part of esters of acrylic acid conditioning agent, diethyl
0.3 part of amino-ethyl ethanol ester, 0.16 part of 6- benzyl gland fats purine, 0.15 part of indolebutyric acid, 0.1 part of methyl α-naphthyl acetate and water, added water
Amount makes the water content of culture medium for cultivating be 58%, and the pH value of the culture medium for cultivating is 6；
S4：Warmhouse booth takes the sunshade net sunshade of 100 mesh, and to control temperature be 20 DEG C, humidity 88%；
S5：Carry out the work of the disasters such as asparagus, the diseases prevention of roxburgh anoectochilus terminal bud, insect prevention；
S6：The Flammulina velutipes mycelium can harvest when cultivating 17cm a length of to entity, and roxburgh anoectochilus terminal bud harvests after cultivating 2 years.
Comparative example 1
It is essentially identical to prepare culture medium for cultivating technique with the step S3 of embodiment 3, have only unlike prepare culture medium for cultivating
Raw material in lack N- lauroyl glutamates potassium, alkyl sulfate sodium, esters of acrylic acid conditioning agent, diethyllaminoethyl ethanol
The planting technique of ester, other asparagus and roxburgh anoectochilus terminal bud is essentially identical.
Comparative example 2
It is essentially identical to prepare culture medium for cultivating technique with the step S3 of embodiment 3, have only unlike prepare culture medium for cultivating
Raw material in lack N- lauroyl glutamate potassium, the planting technique of other asparagus and roxburgh anoectochilus terminal bud is essentially identical.
Comparative example 3
It is essentially identical to prepare culture medium for cultivating technique with the step S3 of embodiment 3, have only unlike prepare culture medium for cultivating
Raw material in lack alkyl sulfate sodium, the planting technique of other asparagus and roxburgh anoectochilus terminal bud is essentially identical.
Comparative example 4
It is essentially identical to prepare culture medium for cultivating technique with the step S3 of embodiment 3, have only unlike prepare culture medium for cultivating
Raw material in lack esters of acrylic acid conditioning agent, the planting technique of other asparagus and roxburgh anoectochilus terminal bud is essentially identical.
Comparative example 5
It is essentially identical to prepare culture medium for cultivating technique with the step S3 of embodiment 3, have only unlike prepare culture medium for cultivating
Raw material in lack diethyllaminoethyl ethanol ester, the planting technique of other asparagus and roxburgh anoectochilus terminal bud is essentially identical.
Using identical young plant, roxburgh anoectochilus terminal bud is planted using embodiment 1-3 and comparative example 1-5 methods, using patent document " gold
The bottled culture medium preparation method of line lotus and its packaging device (publication number：CN103766205A the embodiment culture medium plantation in) "
Roxburgh anoectochilus terminal bud (is set to comparative example 6), and the other conditions such as illumination, humidity are essentially identical, after each group is cultivated 3 months, every group of harvesting
10 plants of roxburgh anoectochilus terminal bud measurement content of taurine, polyoses content, amino acid content average yield and length, are measured wherein setting comparative example 6
Content of taurine, polyoses content, amino acid content average yield and the length obtained is respectively 0,0,0,0, other group contrasts, as a result
It see the table below.
(1) from the data of embodiment 1-3 and comparative example 6, the content of taurine of the roxburgh anoectochilus terminal bud of plantation, polyoses content,
Amino acid content, length increase, and illustrate that the culture medium for cultivating that the present invention prepares is nutritious, collocation is reasonable, with showing
There is technology to compare, the roxburgh anoectochilus terminal bud yield that method of the invention is planted is higher, quality is more excellent (contains more active ingredients：Ox sulphur
Acid, polysaccharide, amino acid etc.).
(2) from embodiment 3 and comparative example 1-5 data, N- lauroyl glutamates potassium, alkyl sulfate sodium, third
Olefin(e) acid esters conditioning agent, diethyllaminoethyl ethanol ester serve synergy in roxburgh anoectochilus terminal bud is cultivated, and improve taurine, more
Sugar, amino acid, length；This is probably that N- lauroyl glutamates potassium can make nutritional ingredient effectively scattered, beneficial to plant absorption；
Alkyl sulfate sodium can make plant effectively absorb nutritional ingredient as a kind of bleeding agent；Esters of acrylic acid conditioning agent can play tune
The effect of section, makes plant fully absorb nutritional ingredient；Diethyllaminoethyl ethanol ester can play synergistic effect, significantly improve battalion
The dispersiveness and permeability formed point, make plant fully absorb nutritional ingredient, improve the taurine, polysaccharide, amino of roxburgh anoectochilus terminal bud
Acid, length.
Above content is it cannot be assumed that present invention specific implementation is confined to these explanations, for the technical field of the invention
Those of ordinary skill for, under the premise of present inventive concept is not departed from, some simple deduction or replace can also be made, all should
When being considered as belonging to the scope of patent protection that is determined by the claims submitted of the present invention.
Claims (10)
- A kind of 1. culture medium, it is characterised in that in units of parts by weight, including following raw material：Rice straw minces 92-108 parts, citrus Juice 20-40 parts, peanut cake 15-22 parts, potassium chloride 4-7 parts, sodium nitrate 3-6 parts, ammonium sulfate 3.5-6 parts, sodium iodate 0.3-0.4 Part, calcium nitrate 0.6-0.8 parts, magnesium sulfate 0.8-1 parts, zinc nitrate 0.3-0.5 parts, manganese chloride 0.3-0.4 parts, Boratex 0.4- 0.6 part, sodium molybdate 0.3-0.5 parts, ferric nitrate 0.2-0.3 parts, copper chloride 0.2-0.4 parts, N- lauroyl glutamate potassium 0.6- 0.8 part, alkyl sulfate sodium 0.3-0.5 parts, esters of acrylic acid conditioning agent 0.2-0.4 parts, diethyllaminoethyl ethanol ester 0.2- 0.3 part, 6- benzyl gland fat purine 0.1-0.16 parts, indolebutyric acid 0.08-0.15 parts, methyl α-naphthyl acetate 0.07-0.1 parts and water.
- 2. culture medium according to claim 1, it is characterised in that institute's amount of water makes the water content of culture medium be 55%- 58%.
- 3. culture medium according to claim 1, it is characterised in that the pH value of the culture medium is 5.6-6.
- 4. a kind of application of culture medium according to claim any one of 1-3 in asparagus and roxburgh anoectochilus terminal bud cultivation, it is special Sign is, the cultural method of the asparagus and roxburgh anoectochilus terminal bud, comprises the following steps：S1：The culture of Flammulina velutipes mycelium：Culture medium for golden mushroom is prepared, in temperature is 120- after culture medium for golden mushroom bottling 125 DEG C, pressure is the 3-5min that sterilized under 150-170KPa, accesses Flammulina velutipes mycelium parent species, is cultivated at 23-26 DEG C, forms institute Flammulina velutipes mycelium is stated, it is standby；S2：Withered tree is erect and punching：Several withered trees are erect in warmhouse booth, described withered tree seeds include mulberry tree, banyan, chestnut One kind in tree, distance is 45-62cm between each withered tree, is then punched on the withered tree, the method for the punching is：Institute The aperture beaten is 2.6-4.2cm, depth 4.8-9.2cm, and 30-80 degree punching obliquely, the spacing of adjacent holes is 8.6-17cm, from Ground 0.4-0.6m；S3：The transplanting of roxburgh anoectochilus terminal bud：Long 4.2-5.3cm roxburgh anoectochilus terminal bud is transplanted into described in step S2 in the hole of withered tree, loaded simultaneously Flammulina velutipes mycelium and culture medium for cultivating；S4：Warmhouse booth takes sunshade net sunshade, control temperature, humidity；S5：Carry out the work of the disasters such as asparagus, the diseases prevention of roxburgh anoectochilus terminal bud, insect prevention；S6：The Flammulina velutipes mycelium can harvest when cultivating 12-17cm a length of to entity, be harvested after roxburgh anoectochilus terminal bud cultivation 1.2-2.
- 5. application of the culture medium according to claim 4 in asparagus and roxburgh anoectochilus terminal bud cultivation, it is characterised in that step S1 Described in culture medium for golden mushroom, in units of parts by weight, including following raw material：Rice straw minces 92-108 parts, orange blossom 20-40 Part, peanut cake 15-22 parts, potassium chloride 4-7 parts, sodium nitrate 3-6 parts, ammonium sulfate 3.5-6 parts, sodium iodate 0.3-0.4 parts, calcium nitrate 0.6-0.8 parts, magnesium sulfate 0.8-1 parts, zinc nitrate 0.3-0.5 parts, manganese chloride 0.3-0.4 parts, Boratex 0.4-0.6 parts, molybdic acid Sodium 0.3-0.5 parts, ferric nitrate 0.2-0.3 parts, copper chloride 0.2-0.4 parts and water.
- 6. application of the culture medium according to claim 5 in asparagus and roxburgh anoectochilus terminal bud cultivation, it is characterised in that added water Amount makes the water content of culture medium for golden mushroom be 60%-64%.
- 7. application of the culture medium according to claim 5 in asparagus and roxburgh anoectochilus terminal bud cultivation, it is characterised in that the gold The pH value of pin mushroom culture medium is 5.5-6.2.
- 8. application of the culture medium according to claim 4 in asparagus and roxburgh anoectochilus terminal bud cultivation, it is characterised in that step S4 Described in the mesh number of sunshade net be 50-100.
- 9. application of the culture medium according to claim 4 in asparagus and roxburgh anoectochilus terminal bud cultivation, it is characterised in that step S4 Described in control temperature be 16-20 DEG C.
- 10. the new cultivation technology of asparagus according to claim 4 and roxburgh anoectochilus terminal bud, it is characterised in that described in step S4 Humidity is 83%-88%.
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