CN107467074A - Growth promotion plant growth regulator - Google Patents
Growth promotion plant growth regulator Download PDFInfo
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- CN107467074A CN107467074A CN201710493538.4A CN201710493538A CN107467074A CN 107467074 A CN107467074 A CN 107467074A CN 201710493538 A CN201710493538 A CN 201710493538A CN 107467074 A CN107467074 A CN 107467074A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses growth promotion plant growth regulator, the conditioning agent composition and its parts by weight are：20 25 parts of bacillus megaterium and its metabolite, 5 10 parts of gibberellic acid.Invention also discloses the preparation method of the conditioning agent.Have the beneficial effect that：Two kinds of composition effect mutually collaborations, plant establishment can be remarkably promoted, the cost of raw material prepared used in conditioning agent is cheap, and the ratio between composition is moderate, it is easily stored, safe and non-toxic, process of preparing is simple, and product quality stability is good, it is cheap, application effect is good, has good economy, is a kind of green technique.
Description
Technical field
The present invention relates to plant growth regulator field, more particularly, to growth promotion plant growth regulator.
Background technology
China is one of most widely used country of plant growth regulator in the world.Plant growth regulator is in regulation agriculture
Growing, improving yield and improvement quality aspect for crop, significant contribution is made that for China's agricultural production and development.According to having
Report, 20 end of the centurys are closed, China is occupied first of the world using the area of plant growth regulator every year.With Traditional agriculture technology phase
Application than, plant growth regulator has the advantages that cost is low, fast, high efficiency of producing effects, saves labour, has become 21 generation
Agricultural of recording, which is realized, overfulfils a production target and improves one of major measure of commodity.
Prior art such as Authorization Notice No. is the B of CN 104886055 Chinese invention patent, discloses a plant growth
Conditioning agent, the conditioning agent include cloxyfonac, fluoridamid, gibberellic acid, eugenol, solubilizer and cosolvent, and the product has association
With the effect of synergy, simple production process, cost is low, but the conditioning agent can cause the destruction of soil texture, and fertility declines, agriculture
Crop quality reduces.
The content of the invention
It is an object of the invention to provide a kind of environmentally friendly, efficient growth promotion plant growth regulator.
The present invention is directed to the problem of being mentioned in background technology, and the technical scheme taken is：Growth promotion plant growth regulating
Agent, the conditioning agent composition and its parts by weight are：Bacillus megaterium and its metabolite 20-25 parts, gibberellic acid 5-10 parts.It is used
Bacillus megaterium derive from Shandong Lv Long Bioisystech Co., Ltd.The cost of raw material used is cheap, easily stored, and two
Ratio between kind of composition is moderate, acts on mutually collaboration, can remarkably promote plant growth, for plant development provide it is excellent
Condition.
Preferably, the preparation method of growth promotion plant growth regulator of the present invention includes test tube slant culture, fermentation
Tank fermented and cultured, compound emulsion.Process is simple, obtains that tunning quality is good, and stability is good, during needed for original
Expect cheap, application effect is good, preparation method is had good economic benefit, and favourable bar is provided for industrial applications
Part.
Preferably, in test tube slant incubation, the production strain that will be stored in sandy soil pipe, sterile working access fine jade
Fat slant medium, it is 6-8 in pH, temperature is to cultivate 32-40h at 25-30 DEG C, and obtained strain is preserved to stand-by, work at 3 DEG C
For the strain source of ferment tank culture.Wherein, the composition of agar slant culture-medium and its parts by weight are：Glucose 3-7 parts, ferment
Female cream 2-5 parts, agar 5-10 parts, water 10-15 parts.Under the conditions of being somebody's turn to do, the growth vigor of strain becomes strong, and physiological character is more stable,
Purebred, the shortening process cycle of certain amount and quality can be quickly obtained, and is provided for follow-up ferment tank culture
Sufficient amount of seed.
Preferably, in ferment tank incubation, it is 0.15-0.2MPa, throughput 30-40m to set tank to press3/
H, 5-10 parts bioactive peptide and 1-3 part strains are added, be 6-8 in pH, temperature is to cultivate 20-25h at 25-30 DEG C, obtains huge bud
Spore bacillus and its metabolite.Wherein, the composition of culture medium and its parts by weight are in ferment tank incubation：Glucose 3-
7 parts, yeast extract 2-5 parts, potassium dihydrogen phosphate 2-6 parts, ammonium sulfate 3-6 parts, magnesium sulfate 1-4 parts, water 10-15 parts.Under the conditions of being somebody's turn to do, hair
The ferment time is short, and fermentation tank utilization rate is high, and microbiological contamination chance is small, and the bacillus megaterium produced and its metabolite quality are high, should
It is good with effect, improve the economic value of technique.
Preferably, the amino acid sequence of the bioactive peptide added in ferment tank incubation is：
HSHACKLCVCVNCSR.Bacillus megaterium can synthesize exotoxin in fermentation tank, influence its metabolism, the activity of addition
Small peptide is combined with ectotoxic active site, is effectively reduced ectotoxic poisonous effect, has been provided for fermentation tank culture
Sharp environment.
Preferably, during compound emulsion, 20-25 parts bacillus megaterium and its metabolite are taken, adds 5-10 parts
Gibberellic acid and 1-3 part organic silicon surfactants, stirring 10min are well mixed, and obtain growth promotion plant growth regulator.This
Under part, various composition can be sufficiently mixed, and the addition of surfactant makes the more uniform stabilization of insecticide, and improves product
In the deposition of plant surface, make drug effect more stable, enhance the lasting period of product.
Compared with prior art, the advantage of the invention is that：The conditioning agent of promotion plant establishment disclosed by the invention is main
It is made up of gibberellic acid, bacillus megaterium and its metabolite, acts on mutually collaboration, plant growth can be remarkably promoted；Fermentation
The bacillus megaterium that is incorporated as of bioactive peptide provides good metabolism environment in tank incubation；Prepare used in conditioning agent
The cost of raw material is cheap, and the ratio between various compositions is moderate, easily stored, and process is simple, and product quality stability is good,
Cheap, application effect is good, has good economy, is a kind of green technique.
Embodiment
Below by embodiment, the present invention will be further described：
Embodiment 1：
Growth promotion plant growth regulator, the conditioning agent composition and its parts by weight are：Bacillus megaterium and its metabolite
20 parts, 5 parts of gibberellic acid.Bacillus megaterium used derives from Shandong Lv Long Bioisystech Co., Ltd.The system of the mediator agent
Preparation Method includes：It is test tube slant culture first：Alcolhol burner is lighted, oese is sterilized by flame calcination, by connecing for sterilizing
Kind of ring is stretched into strain tube, and ring first is contacted into inside pipe wall, reaches the purpose of cooling, then a little lawn of picking again.By oese
Strain tube is exited, stretches into slant tube to be seeded rapidly, straight line is lightly drawn from the bottom up end of test tube on inclined-plane with ring,
Culture medium is not scratched, also not make ring contact tube wall or the mouth of pipe, oese exits slant tube, then burns the mouth of pipe with flame, and
Flame side by test tube plug beyond the Great Wall, pH be 6, temperature be 25 DEG C at cultivate 32h, it is stand-by by being preserved at obtained 3 DEG C of strain, as
The strain source of ferment tank culture.Wherein, the composition of agar slant culture-medium and its parts by weight are：3 parts of glucose, yeast extract
2 parts, 5 parts of agar, 10 parts of water.Under the conditions of being somebody's turn to do, the growth vigor of strain becomes strong, and physiological character is more stable, can quickly obtain
Certain amount and quality it is purebred, provide sufficient amount of seed for follow-up ferment tank culture；Followed by fermentation tank
Fermented and cultured：Fermentation tank valve is closed, sealing is checked, is passed through steam and pipeline is carried out disinfection, adds 5 parts of bioactive peptides and 1
Part strain, it is 0.15MPa, throughput 30m to set tank to press3/ h, it is 6 in pH, temperature is to cultivate 20h at 25 DEG C, obtains huge bud
Spore bacillus and its metabolite.Wherein, the composition of culture medium and its parts by weight are in ferment tank incubation：Glucose 3
Part, 2 parts of yeast extract, 2 parts of potassium dihydrogen phosphate, 3 parts of ammonium sulfate, 1 part of magnesium sulfate, 10 parts of water.Under the conditions of being somebody's turn to do, fermentation time is short, hair
Fermentation tank utilization rate is high, and microbiological contamination chance is small, and the bacillus megaterium produced and its metabolite quality are high, improve the warp of technique
Ji value；It is finally compound emulsion：20 parts of bacillus megateriums and its metabolite are taken, adds 5 parts of gibberellic acid and 1 part of organosilicon
Surfactant, stirring 10min are well mixed, and obtain growth promotion plant growth regulator.The addition of surfactant makes production
The more uniform stabilization of conditioning agent, improve effect of the product in actual application.
Embodiment 2：
Growth promotion plant growth regulator, the conditioning agent composition and its parts by weight are：Bacillus megaterium and its metabolite
22 parts, 7 parts of gibberellic acid.Bacillus megaterium used derives from Shandong Lv Long Bioisystech Co., Ltd.The system of the mediator agent
Preparation Method includes：
1) test tube slant culture：The production strain that will be stored in sandy soil pipe, sterile working access agar slant culture-medium, in pH
For 7, temperature is to cultivate 35h at 26 DEG C, and obtained strain is preserved to stand-by, the strain source as ferment tank culture at 3 DEG C.
Wherein, the composition of agar slant culture-medium and its parts by weight are：4 parts of glucose, 3 parts of yeast extract, 6 parts of agar, 12 parts of water.This
Under part, the growth vigor of strain becomes strong, and physiological character is more stable, can quickly obtain the purebred of certain amount and quality,
Sufficient amount of seed is provided for follow-up ferment tank culture；
2) ferment tank culture：It is 0.17MPa, throughput 35m to set tank to press3/ h, add 6 parts of bioactive peptides and 2 parts of bacterium
Kind, it is 7 in pH, temperature is to cultivate 22h at 27 DEG C, obtains bacillus megaterium and its metabolite.Wherein, ferment tank culture
During culture medium composition and its parts by weight be：4 parts of glucose, 3 parts of yeast extract, 3 parts of potassium dihydrogen phosphate, 4 parts of ammonium sulfate, sulphur
Sour 2 parts of magnesium, 12 parts of water.Under the conditions of being somebody's turn to do, fermentation time is short, and fermentation tank utilization rate is high, and microbiological contamination chance is small, the huge gemma produced
Bacillus and its metabolite quality are high, improve the economic value of technique；
3) compound emulsion：22 parts of bacillus megateriums and its metabolite are taken, 7 parts of gibberellic acid is added and 2 parts of organosilyl surfaces is lived
Property agent, stirring 10min be well mixed, obtain growth promotion plant growth regulator.The addition of surfactant makes the regulation of production
The more uniform stabilization of agent, improves effect of the product in actual application.
Embodiment 3：
Growth promotion plant growth regulator, the conditioning agent composition and its parts by weight are：Bacillus megaterium and its metabolite
25 parts, 10 parts of gibberellic acid.Bacillus megaterium used derives from Shandong Lv Long Bioisystech Co., Ltd.The system of the mediator agent
Preparation Method includes：
1) test tube slant culture：The production strain that will be stored in sandy soil pipe, sterile working access agar slant culture-medium, in pH
For 8, temperature is to cultivate 40h at 30 DEG C, and obtained strain is preserved to stand-by, the strain source as ferment tank culture at 3 DEG C.
Wherein, the composition of agar slant culture-medium and its parts by weight are：7 parts of glucose, 5 parts of yeast extract, 10 parts of agar, 15 parts of water.Should
Under the conditions of, the growth vigor of strain becomes strong, and physiological character is more stable, can quickly obtain the pure of certain amount and quality
Kind, provide sufficient amount of seed for follow-up ferment tank culture；
2) ferment tank culture：It is 0.2MPa, throughput 40m to set tank to press3/ h, add 10 parts of bioactive peptides and 3 parts of bacterium
Kind, it is 8 in pH, temperature is to cultivate 25h at 30 DEG C, obtains bacillus megaterium and its metabolite.Wherein, ferment tank culture
During culture medium composition and its parts by weight be：7 parts of glucose, 5 parts of yeast extract, 6 parts of potassium dihydrogen phosphate, 6 parts of ammonium sulfate, sulphur
Sour 4 parts of magnesium, 15 parts of water.Under the conditions of being somebody's turn to do, fermentation time is short, and fermentation tank utilization rate is high, and microbiological contamination chance is small, the huge gemma produced
Bacillus and its metabolite quality are high, improve the economic value of technique；
3) compound emulsion：25 parts of bacillus megateriums and its metabolite are taken, 10 parts of gibberellic acid is added and 3 parts of organosilyl surfaces is lived
Property agent, stirring 10min be well mixed, obtain growth promotion plant growth regulator.The addition of surfactant makes the regulation of production
The more uniform stabilization of agent, improves effect of the product in actual application.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should be included in the scope of the protection.
SEQUENCE LISTING
<110>Pujiang County Ang Bao Bioisystech Co., Ltd
<120>Growth promotion plant growth regulator
<130> 1
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 15
<212> PRT
<213>It is artificial synthesized
<400> 1
His Ser His Ala Cys Lys Leu Cys Val Cys Val Asn Cys Ser Arg
1 5 10 15
Claims (7)
1. growth promotion plant growth regulator, it is characterised in that：Described conditioning agent composition and its parts by weight are：Huge gemma
Bacillus and its metabolite 20-25 parts, gibberellic acid 5-10 parts.
2. growth promotion plant growth regulator according to claim 1, its preparation method includes test tube slant culture, hair
Fermenting pot culture, compound emulsion, it is characterised in that：In described ferment tank incubation, it is 0.15- to set tank to press
0.2MPa, throughput 30-40m3/ h, 5-10 parts bioactive peptide and 1-3 part strains are added, is 6-8, temperature 25-30 in pH
40-45h is cultivated at DEG C, obtains bacillus megaterium and its metabolite.
3. the preparation method of growth promotion plant growth regulator according to claim 2, it is characterised in that：Described hair
The amino acid sequence of the bioactive peptide added in fermenting pot incubation is：HSHACKLCVCVNCSR.
4. the preparation method of growth promotion plant growth regulator according to claim 2, it is characterised in that：Described hair
In fermenting pot incubation, the composition and its parts by weight of culture medium are：Glucose 3-7 parts, yeast extract 2-5 parts, biphosphate
Potassium 2-6 parts, ammonium sulfate 3-6 parts, magnesium sulfate 1-4 parts, water 10-15 parts.
5. the preparation method of growth promotion plant growth regulator according to claim 2, it is characterised in that：Described examination
During pipe inclined-plane culture, the bacillus megaterium in sandy soil pipe will be stored in, sterile working accesses agar slant culture-medium,
PH is 6-8, and temperature is to cultivate 32-40h at 25-30 DEG C, the strain source using obtained strain as ferment tank culture.
6. the preparation method of growth promotion plant growth regulator according to claim 2, it is characterised in that：Described examination
During pipe inclined-plane culture, the composition and its parts by weight of agar slant culture-medium are：Glucose 3-7 parts, yeast extract 2-5 parts, fine jade
Fat 5-10 parts, water 10-15 parts.
7. the preparation method of growth promotion plant growth regulator according to claim 2, it is characterised in that：Described answers
With 20-25 parts bacillus megaterium and its metabolite in emulsion process, is taken, 5-10 parts gibberellic acid and 1-3 part organosilicons are added
Surfactant, it is stirred, obtains growth promotion plant growth regulator.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710493538.4A CN107467074A (en) | 2017-06-26 | 2017-06-26 | Growth promotion plant growth regulator |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710493538.4A CN107467074A (en) | 2017-06-26 | 2017-06-26 | Growth promotion plant growth regulator |
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| CN107467074A true CN107467074A (en) | 2017-12-15 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108617713A (en) * | 2018-07-18 | 2018-10-09 | 合肥百绿盛农业科技有限公司 | A kind of preparation method of nuisanceless composite plant growth regulator |
| CN108739889A (en) * | 2018-07-18 | 2018-11-06 | 合肥百绿盛农业科技有限公司 | A kind of sweet osmanthus nursery stock plant growth regulator |
| CN108782623A (en) * | 2018-07-18 | 2018-11-13 | 合肥百绿盛农业科技有限公司 | Efficient Yield increasing type plant growth regulator and its application |
| CN110423746A (en) * | 2019-07-16 | 2019-11-08 | 北京林业大学 | The method for encoding gene order, small peptide PFER and the application of small peptide PFER and inducing plant establishment |
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| CN1757716A (en) * | 2005-07-25 | 2006-04-12 | 中国林业科学研究院热带林业研究所 | Coculturing fermentation method of root nodule bacterial and Bacillus phosphorus |
| CN101724560A (en) * | 2009-11-23 | 2010-06-09 | 山东盛隆生物工程有限公司 | Microbial inoculums and preparation method thereof |
| CN104447032A (en) * | 2014-12-15 | 2015-03-25 | 苏州科大微龙信息技术有限公司 | Gibberellin-containing hydroponic leaf vegetable organic nutrient solution and preparation method of nutrient solution |
| CN105263965A (en) * | 2013-03-15 | 2016-01-20 | 斯波根生物技术公司 | Fusion proteins and methods for stimulating plant growth, protecting plants, and immobilizing bacillus spores on plants |
-
2017
- 2017-06-26 CN CN201710493538.4A patent/CN107467074A/en not_active Withdrawn
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1757716A (en) * | 2005-07-25 | 2006-04-12 | 中国林业科学研究院热带林业研究所 | Coculturing fermentation method of root nodule bacterial and Bacillus phosphorus |
| CN101724560A (en) * | 2009-11-23 | 2010-06-09 | 山东盛隆生物工程有限公司 | Microbial inoculums and preparation method thereof |
| CN105263965A (en) * | 2013-03-15 | 2016-01-20 | 斯波根生物技术公司 | Fusion proteins and methods for stimulating plant growth, protecting plants, and immobilizing bacillus spores on plants |
| CN104447032A (en) * | 2014-12-15 | 2015-03-25 | 苏州科大微龙信息技术有限公司 | Gibberellin-containing hydroponic leaf vegetable organic nutrient solution and preparation method of nutrient solution |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108617713A (en) * | 2018-07-18 | 2018-10-09 | 合肥百绿盛农业科技有限公司 | A kind of preparation method of nuisanceless composite plant growth regulator |
| CN108739889A (en) * | 2018-07-18 | 2018-11-06 | 合肥百绿盛农业科技有限公司 | A kind of sweet osmanthus nursery stock plant growth regulator |
| CN108782623A (en) * | 2018-07-18 | 2018-11-13 | 合肥百绿盛农业科技有限公司 | Efficient Yield increasing type plant growth regulator and its application |
| CN110423746A (en) * | 2019-07-16 | 2019-11-08 | 北京林业大学 | The method for encoding gene order, small peptide PFER and the application of small peptide PFER and inducing plant establishment |
| CN110423746B (en) * | 2019-07-16 | 2021-03-12 | 北京林业大学 | Gene sequence for coding small peptide PFER, application and method for inducing plant to root |
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Application publication date: 20171215 |