CN104529613B - Organic-biological compounds liquid fertilizer and alkaline land improving application process - Google Patents
Organic-biological compounds liquid fertilizer and alkaline land improving application process Download PDFInfo
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- CN104529613B CN104529613B CN201410717310.5A CN201410717310A CN104529613B CN 104529613 B CN104529613 B CN 104529613B CN 201410717310 A CN201410717310 A CN 201410717310A CN 104529613 B CN104529613 B CN 104529613B
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C9/00—Fertilisers containing urea or urea compounds
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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Abstract
The present invention relates to a kind of organic-biological compounding liquid method for preparing fertilizer and alkaline land soil improvement application process, belong to green liquid organic fertilizer and alkaline land soil improving technology field.According to the parts by weight of raw material, 1 50 parts of amino-acid liquid fertilizer is taken, 1 50 parts of lactic acid bacteria Liquid Fertilizer is mixed evenly in room temperature；Amino acid contained 200 220g/L of total amount of the amino-acid liquid fertilizer；1,000,000,000/mL of lactic acid bacteria Liquid Fertilizer viable bacteria content.The present invention is combined by using two kinds of the effective of organic fertilizer, and the effect of 1+1 is more than 2 can be played to the nutritional ingredient of plant.
Description
Technical field
The present invention relates to a kind of organic-biological compounding liquid method for preparing fertilizer and alkaline land soil improvement application process, belong to
In green liquid organic fertilizer and alkaline land soil improving technology field.
Background technology
According to China's development low-carbon, circulation, organic agriculture, rescue, improvement, repair arable land；Ensure national food security.Protect
Hinder the agricultural development planning of food security, in line with the purpose for improving quality of agricultural product, reducing production cost, research crops green
Biological organic fertilizer becomes a kind of inexorable trend.Biological organic Liquid Fertilizer is also one kind of biological organic fertilizer.
Biological organic fertilizer is used as the green animal and plant body extract of fertilizer.Biological organic fertilizer is that a kind of nutrient is completely green
The colour biological source of manure.Biological organic fertilizer is not only to add the effective ways of the source of manure, also has great role to improvement soil, specific next
Say, biological organic liquid fertilizer has following advantage：
(1) abundant nutrient can be provided for soil.Various animal and plant bodies, containing abundant nutrient, once in biochemistry
Micromolecule polypeptide organic matter is isolated under effect, organic matter in soil and nitrogen, phosphorus, potassium, calcium, magnesium and various can be increased in large quantities
Trace element.
(2) amino acid and lactic acid bacteria can convert difficultly soluble nutrient in soil, in favor of absorbing for crop.Biology has
Machine Liquid Fertilizer, which decomposes the organic acid produced, can enable the phosphorus of slightly solubility, potassium in soil be converted into validity phosphorus, potassium that crop utilizes.
(3) physical and chemical character of soil can be improved.After biological organic fertilizer is manured into soil, under the action of microorganism, no
Decompose disconnectedly, in addition to mass efficient nutrient is discharged, also form humus, humus is combined with calcium can make soil cementation into granule
Structure, has the loosing soil of crumb structure, breathes freely, retain water and nutrients power is strong, adjusts water, fertilizer, the performance of air and heat；Particularly amino
The pH value that acid and lactic acid bacteria can adjust alkaline land soil tends to neutral soil；Improvement to alkaline land soil has very good
Application value；The salination and the saliferous improvement of this life for being conducive to the northern area of China arable land are repaired.
(4) activity of edaphon is promoted.After biological organic fertilizer is manured into soil, fresh organic energy substance is added,
Microorganism is set to breed rapidly, activity enhancing, promotes the formation of humus, the validation of nutrient, accelerates soil-ripening.
Biological organic Liquid Fertilizer has been studied in China at present, such as number of patent application：200980158552.2 disclose one
The Amino acid liquid fertilizer that kind is prepared the method for Amino acid liquid fertilizer and thus prepared using poultry blood.1) poultry blood is collected
Liquid is simultaneously ground poultry blood；2) protease extracted in soybean is added in the blood by grinding, albumen will be added
The blood of enzyme, which is put into, to be kept in 50-70 DEG C of reactor, when being reacted that about 8-15 is small under conditions of closed, as the PH of blood
It is worth to terminate reaction during 7.5-8.0, makes reactant cooling when about 3-4 is small.3) in single mixer at about 25 DEG C -80 DEG C
In, urea, potash, diammonium hydrogen phosphate, water-based zinc and EDTA are added to the water, are thoroughly mixed the mixture to prepare additive
Mixture solution.4) using obtained blood in 100 mesh filters filtering second step, by additive agent mixture made from the 3rd step
Solution is mixed with the filtered blood.
Safety non-pollution of the present invention, organo-mineral complexing improve nutrient and efficiently use, can be widely used in corn,
The industrial crops such as cotton, rape, peanut, beans and silkworm and mulberry, tea woods, fruit tree, various vegetables.
Such as number of patent application：201010503246.2 disclose a kind of processing method of organic liquid fertilizer.
By the waste water of production bean curd generation, waste residue according to weight ratio 1:0.3-0.6 puts into fermentation tank, at 103-105 DEG C
Sterilization 60-80 minutes under high temperature, 30-40 DEG C is then cooled to, culture medium is made.By brewer's yeast, bacillus subtilis
With lactic acid bacteria according to 1：1:1.2 ratio mixing, is made mixed bacteria.Ratio according to the 1.0-1.8% of culture medium weight takes
Above-mentioned steps 2) mixed bacteria, mixed bacteria is uniformly sprayed onto in culture medium.Cultivated at 35-40 DEG C of temperature, until culture
Bubble-free produces in base fermentation tank, that is, generates organic liquid fertilizer.
But the fertilizer of above-mentioned preparation is not strong to salt-soda soil specific aim, it is impossible to effectively solves the continuous cropping barrier in agricultural production
Hinder problem, existing lactic acid bacteria, which is unable to rehabilitating soil colloid and crumb structure character, improves pH value；Repair and improve soil physics matter
Amount standard, it is impossible to while improve the amino acid in soil and lactic acid and animal/vegetable protein rehabilitating soil colloid, it is impossible to logical induction is planted
Thing produces sozin (alkaloids, flavonoid class, protein-based, organic acid and phenolic compound)
Current existing biological organic liquid fertilizer is not specific to alkaline land soil improvement to develop, its specific aim
It is not strong.
The content of the invention
For above the deficiencies in the prior art, the present invention provides a kind of organic-biological compounding alkaline land soil improvement liquid
Method for preparing fertilizer and alkaline land soil improvement application process.The biological organic liquid fertilizer realizes " turning waste into wealth ", " green
Color ", has the function of to improve alkaline land soil physicochemical character, low-carbon safety；And it can supply comprehensively organic, micro- needed for plant
Measure nutrient.
The technical scheme is that：A kind of organic-biological compounds liquid fertilizer, according to the parts by weight of raw material, takes amino acid
1-50 parts of Liquid Fertilizer, 1-50 parts of lactic acid bacteria Liquid Fertilizer are mixed evenly in room temperature；The amino-acid liquid fertilizer is amino acid contained
Total amount 200-220g/L；1,000,000,000/mL of lactic acid bacteria Liquid Fertilizer viable bacteria content.
Further, the amino-acid liquid fertilizer, weight share forms the present invention program：The feather of uniform state is taken to hang
1000~3000 parts of supernatant liquid；3942 5~30 parts of neutral proteinases；1398 5~30 parts of neutral proteinases；166 unwrapping wire mycoproteinases 5
~30 parts；2~10 parts of pancreatin；5~30 parts of urea；1-10 parts of sodium hydrogensulfite；Manganese chloride 1~10.
The amino-acid liquid fertilizer is prepared by following steps：
A., poultry feather is prepared into the feather suspension of uniform state；
B. by the feather suspension placing response kettle prepared, urea, sodium hydrogensulfite, pancreatin are sequentially added, is maintained
The mixing speed stirring 2h of 180 turns/min, and 45-47 DEG C is warming up to, 3942 neutral proteinases and 1398 neutral proteinases are added,
Suspension is made in addition 166 unwrapping wire mycoproteinase of enzyme preparation after maintaining the mixing speed 12h of 180 turns/min, uses 30% hydrogen-oxygen
Change calcium and adjust pH value to 6.8-7.2, and chlorination is added after continuation enzyme hydrolysis 18h under 1.1 × 105~1.1 × 110Pa pressure
Manganese, with 12mol/L sulphur acid for adjusting pH value to 5.0, is heated to 76-80 DEG C of insulation 5h, 600 mesh mistake of pressure glass fibre filter bag machine
Filter, the filter residue being collected into is rich in amino acid and various trace elements and organic matter is the green organic fertilizer of vegetables, flowers, collection
Filtrate is exactly enzyme hydrolysis poultry feather Amino acid liquid fertilizer, aseptic subpackaged storage, the Amino acid liquid fertilizer of prepared completion
Amino acid contained total amount 200-220g/L.
Further, feather suspension is prepared as：
1) poultry feather is collected, through impurity elimination, cleaning, stopped clearly to water, placed at aeration-drying and receive solar irradiation and dry in the air
It is dry, after its aqueous about 8-10%, then place in baking oven and dried, drying temperature is set in 47-52 DEG C and obtains dry feather；
2) the drying feather of preparation is placed to the sheet that 1-3mm is ground into pulverizer, example in mass ratio prepares mixed liquor,
Powder Yu Mao ︰ distilled water is 1 ︰ 5, and the mixed liquor after compounding is placed on and the sheet of 0.02-0.03mm is ground into colloid mill mixes
Liquid is closed, then sheet mixed liquor is placed to the feather suspension that uniform state is prepared into homogeneous machine.
The lactic acid bacteria Liquid Fertilizer, raw material are according to parts by weight composition：Edible 1-10 parts of white granulated sugar, eats brown sugar 1-10
Part, 1-10 parts of analysis for soybean powder, 1-3 parts of xanthans, 1-3 parts of converted starch, 1-2 parts of sucrose ester, high temperature ɑ-starch liquefacation enzyme 0.01-
0.03 part, 0.001-0.003 parts of sodium pyrophosphate, 0.01-0.05 parts of dipotassium hydrogen phosphate, above-mentioned material is mixed, adds purified water 73-
95 parts, 0.01-0.02 parts of lactobacillus bulgaricus, 0.01-0.02 parts of streptococcus thermophilus.
Further, the lactic acid bacteria Liquid Fertilizer produces the lactic acid bacteria liquid fertilizer according to the following steps：
1) by weight by edible white granulated sugar 1-10 parts, edible 1-10 parts of brown sugar, 1-3 parts of xanthans of 1-10 parts of analysis for soybean powder,
1-3 parts of converted starch, 1-2 parts of sucrose ester, high temperature ɑ -0.01-0.03 parts of starch liquefacation enzyme, 0.001-0.003 parts of sodium pyrophosphate,
0.01-0.05 parts of dipotassium hydrogen phosphate, above-mentioned material is mixed, and is added 73-95 parts of purified water to place and is prepared into homogeneous machine uniformly
The suspension of state, then it is used as culture medium after carrying out high-temperature sterilization processing.
2) one of following strain or both combination is inoculated with when liquid medium being cooled to 35-40 DEG C, according to parts by weight, is connect
Kind measures 0.01-0.04 parts；The strain is 0.01-0.02 parts of lactobacillus bulgaricus, 0.01-0.02 parts of streptococcus thermophilus；
3) the inoculation mixed liquor of step (2) is placed in culture tank, when keeping 35-38 DEG C of culture 48 small, pH value reaches
During 3.3-4.5, glass cloth filtering, collects the preparation that filtrate completes lactic acid bacteria liquid fertilizer；
4) by the lactic acid bacteria liquid fertilizer of preparation it is aseptic subpackaged, storage.
Further, high-temperature process described in step 1) is when keeping 30-120min sterilizings at a temperature of 110-130 DEG C
Between.
A kind of alkaline land improving application process of organic-biological compounding liquid fertilizer, when carrying out alkaline land soil improvement administration
To rush and apply with water；The best application process of fertilizer efficiency is：Crop field irrigation water is first imported and compounds the organic-biological of preparation with fertile pond
Alkaline land soil improve liquid fertilizer according to：The multiple of the ︰ 3000 of Fei Liao ︰ water=1 is added to fertile pond；Stagnate 2-4h and treat that liquid manure fills
Point mixing and water temperature lifted 0.5-3 DEG C when carry out crop field irrigation.
Organic-biological compounding liquid fertilizer is used according to 25-100 kgs/acre of (667 ㎡) amount of application.
Feather of the present invention refers to poultry (chicken and duck goose).
The present invention will be further explained below：
The Mechanism of fertilizer efficiency of poultry feather selected by 1.：
(1) poultry feather Advances on Effective Compositions：
In recent years, with the continuous improvement of detection technique, and attention of the country to amino acid medicinal study, according to Shanghai
University Zhu Xian, Yang Lei, which research and analyse, shows that poultry feather contains：Lysine, proline, methionine, threonine, tryptophan, group ammonia
Amino acid necessary to more than the 16 kinds animals and plants such as acid, glycine, cystine, also containing macroelement, trace element, vitamin
And unknown growth factor (magnifying thunder).Monomer of the amino acid as protein, its application prospect is very wide, feather protein matter
It is a kind of insoluble keratin, there is stronger stability, is difficult hydrolysis under normal circumstances.
(2) microorganism growth is promoted:
Each quasi-microorganism is required for a variety of nutriments during its vital movement in nature, mainly including water, carbon
Source, nitrogen source, mineral element, auxin etc..And growth of the auxin for microorganism has great importance, because they are micro-
The prothetic group component of enzyme classes in biology, once lacking these materials, enzyme does not just have vigor, and the vital movement of microorganism also just stops
.And ion, the auxin needed for the multiple-microorganism such as saccharomycete contained in amino acid, these microorganisms can be advantageously promoted
Growth and breeding.
(3) bacteriostasis of amino acid：
Domestic and international agricultural experts are sprayed on plant using Class of Amino Acid such as tryptophans resists and eliminates farming
The action effect of thing germ；According to a variety of medicine and pharmacology document reports, polypeptide (general name of amino acid) is to shigella dysenteriae, diphtheria bar
Bacterium, proteus, bacillus typhi murium, green expanding rod bacterium, Escherichia coli, staphylococcus aureus, hay bacillus, cured sample bacillus, eight
A variety of pathogenic microbes such as folded bacillus have good inhibiting effect.
2nd, the nutrition Mechanism of fertilizer efficiency of selected amino acid：
1) be first amino acid physiological function：Amino acid is connected as peptide and protein by peptide bond.Amino acid,
Peptide and protein are the histiocytic ultimate constituents of organic life entity, play life movement very important work
With.Some amino acid also participate in some special metabolic responses in addition to it can form protein, show some important life sections
Learn characteristic ...
2) dissociation property of amino acid forms the distinctive pH value acid-base balance function of amino acid：Amino acid is in crystal habit
Or in aqueous, do not exist with free carboxyl or amine-format, but be dissociated into amphion.In amphion, ammonia
Base is to protonate (- NH3 +) form presence, carboxyl is with dissociated state (- COO-) exist.Under different pH conditions.Both sexes
The state of ion also changes therewith.Amino acid is also equipped with both sexes dissociation property and isoelectric point, when Freamine Ⅲ is a certain
When determining pH value, make on certain specific amino acid molecule institute's band positive and negative charge equal, become amphion；So as to balance alkaline land soil
PH value progressively tend to neutral.And amino acid is from the influence of Na+ and Na- ions and pH value in fused soil and dry
Disturb, other nutrients that amino acid is linked can directly be absorbed by crop.
3) Amino acid liquid fertilizer is used for crops, tool be significantly improved yield, improving quality, reduce pesticide residue,
The effect of preserving the ecological environment etc..
3rd, selected lactic acid bacteria is to belong to lactic acid Cordycepps, and this kind of bacterium is morphologically widely different, existing elongated rod shape,
Rod-short has circular again；So species is all gram-positive bacteria, gemma is not formed, and is facultative anaerobic bacteria, is adapted to
Oxygen content is low or oxygen-free environment under grow, organic acid can be largely discharged under alkaline land soil to complete lactic acid bacteria existence and passage
Existence demand；The pH value that the organic acid that lactic acid bacteria carries and generates can improve salt-soda soil progressively tends to neutral.With scientist couple
Lactic acid bacteria improvement, the further research of repair saline-land soil environment；Believe and necessarily have very big harvest.
Compared with prior art, advantage of the invention is that：
The present invention mainly realizes that organic agriculture plantation is realized in alkaline land soil improvement (neutral pH) on salt-soda soil
High yield increases income (upgrading drop is originally).Without using with few future using chemical fertilizer, the guarantee organic agriculture of Maximum Safety
It can harvest and increase income.
Problem on obstacle does in the company solved in agricultural production.Show：Increase soil organic matter content and trace element.Especially
It is to increase the organic nutrition element that plant reproductive growth and nutrient growth must absorb：Such as glucose, fructose, oligosaccharide,
Nucleotide, vitamin, amino acid etc..These organic nutritioies are all by being provided in amino acid and the humification process of lactic acid bacteria
To plant growth overall process.
The effective element possessed by organic substance itself suppresses the harmful microorganism in soil, improves edaphon
Environment, reaches reparation and improves the chemical quality standard of soil.
By increasing amino acid and lactic acid bacteria and animal/vegetable protein rehabilitating soil colloid and crumb structure character in soil,
Improve P in soil H values；Reach reparation and improve soil physics quality standard.
There is certain preventive and therapeutic effect to soil-borne disease.Because biological source bactericide is some using containing in some plants
The plant defense element that antibacterial material or induction produce, kills or effectively suppresses the growth and development of some pathogens.In plant
Antimicrobial compound is a variety of secondary metabolites with antibacterial activity that plant produces, and contains alkaloids, flavonoids
Many different types such as class, protein-based, organic acid and phenolic compound, such as capillin (Capillin), gleditsia sinensis glycoside
(Saponin)。
The organic fertilizer made using this method have selected bacterial strain is clear and definite, effective viable bacteria Tiny ecosystem is stable, more than content and
The advantage simple production process such as bacterial metabolism thing application effect is obvious, comprehensive low into wood, fertilizer is applied widely.
Combined by using two kinds of the effective of organic fertilizer, the effect of 1+1 is more than 2 can be played to the nutritional ingredient of plant.
Embodiment
Embodiment 1：
A kind of organic-biological compounding alkaline land soil improvement liquid fertilizer described in the present embodiment, by following parts by weight (kg)
Raw material composition：According to the parts by weight of raw material, 50 parts of amino-acid liquid fertilizer is taken, 50 parts of lactic acid bacteria Liquid Fertilizer is mixed in (25 DEG C) of room temperature
Conjunction stirs evenly；The amino acid contained total amount 200-220g/L of the amino-acid liquid fertilizer；Lactic acid bacteria Liquid Fertilizer viable bacteria content 1,000,000,000/
mL。
Mixed liquor is sterile, packing；Prepared with regard to completing a kind of organic-biological compounding alkaline land soil improvement fertilizer.
Wherein amino-acid liquid fertilizer, is made of the raw material of following parts by weight (kg)：Take the feather suspension of uniform state
3000 parts；3942 15 parts of neutral proteinases；1398 7.5 parts of neutral proteinases；166 7.5 parts of unwrapping wire mycoproteinases；3 parts of pancreatin；Urine
15 parts of element；10 parts of sodium hydrogensulfite；3 parts of manganese chloride.
Preparation method comprises the following steps：Match and operate according to parts by weight of raw materials：
Step 1)：After the collection of birds (chicken, duck, goose) feather, impurity elimination, cleaning (clear water)；Place at aeration-drying and connect
Dried by solar irradiation to aqueous about 8-10%, then place secondary drying in baking oven, drying temperature is set in 47-52 DEG C.
Dry feather is placed in pulverizer and is ground into 1-3mm by step 2)；Example prepares intermixture powder Yu Mao ︰ in mass ratio
The ︰ 5 of distilled water=1, the intermixture after compounding is placed in colloid mill and is ground into 0.02-0.03mm mixed liquors, then mixed liquor is put
Put the feather suspension that uniform state is prepared into homogeneous machine.
Step 3) prepares amino acid by enzymatic reaction (enzyme hydrolysis).Use enzyme preparation species：3942 neutral proteinases are (living
Power 200000U/g)；1398 neutral proteinases (vigor 30000U/g)；166 unwrapping wire mycoproteinases (vigor 30000U/g)；Pancreatin
(vigor of apoenzyme vigor and coenzyme ratio is 1 in pancreatin:60).
3 tons of placement 5m of feather suspension that step 4) will prepare3Reaction kettle in, add urea 15kg, bisulfite
Sodium 10kg, pancreatin 3kg maintain the mixing speed stirring 2h of 180 turns/min and are warming up to 45-47 DEG C of 3942 neutral proteinases of addition
15kg and 1398 neutral proteinase 7.5kg, 166 unwrapping wire mycoprotein of enzyme preparation is added after maintaining the mixing speed 12h of 180 turns/min
Enzyme 7.5kg, suspension is adjusted and maintain pH6.8-7.2 using 30% calcium hydroxide；Maintain 1.1 × 105~1.1 ×
110Pa pressure continues enzyme hydrolysis 18h and adds manganese chloride 3kg, with 12mol/L sulphur acid for adjusting pH to 5.0, is heated to 76-80 DEG C of guarantor
Warm 5h, the filtering of 600 mesh of pressure glass fibre filter bag machine, the filter residue being collected into are rich in amino acid and various trace elements and organic matter
It is the green organic fertilizer of vegetables, flowers.The filtrate of collection is exactly enzyme hydrolysis poultry feather amino-acid liquid fertilizer；Aseptic subpackaged storage
.The amino acid contained total amount 200-220g/L of amino-acid liquid fertilizer of prepared completion.
Lactic acid bacteria Liquid Fertilizer, is made of the raw material of following parts by weight (kg)：By weight by edible 10 parts of white granulated sugar, food
With 10 parts of brown sugar, 1 part of analysis for soybean powder, 1 part of xanthans, 1 part of converted starch, 2 parts of sucrose ester (SE), high temperature ɑ-starch liquefacation enzyme
0.001 part, 0.001 part of sodium pyrophosphate, 0.01 part of dipotassium hydrogen phosphate, above-mentioned material is mixed, adds purified water 85, bulgarian milk
0.01 part of bacillus, 0.01 part of streptococcus thermophilus
Preparation method comprises the following steps：Match and operate according to parts by weight of raw materials：
1) edible 10 parts of white granulated sugar is eaten into 10 parts of brown sugar, 1 part of analysis for soybean powder, 1 part of xanthans, converted starch 1 by weight
Part, 2 parts of sucrose ester (SE), high temperature ɑ -0.001 part of starch liquefacation enzyme, 0.001 part of sodium pyrophosphate, 0.01 part of dipotassium hydrogen phosphate, will
Above-mentioned material mixes, and adds 85 parts of purified water to place in homogeneous machine and is prepared into the suspension of uniform state, then carries out high-temperature sterilization
Processing (high-temperature process is that 30-120min sterilization times are kept at a temperature of 110-130 DEG C) is used as culture medium afterwards.
2) combination of both following strains is inoculated with when above-mentioned liquid medium being cooled to 35-40 DEG C, inoculum concentration is 0.02 part of (weight
Measure part).0.01 part of lactobacillus bulgaricus, 0.01 part of streptococcus thermophilus.
3) the inoculation mixed liquor of step (2) is placed in culture (fermentation) tank, when keeping 35-38 DEG C of culture 48 small, pH value
When reaching 3.3-4.5, glass cloth filtering, collects the preparation that filtrate completes lactic acid bacteria liquid fertilizer.
1,000,000,000/mL of lactic acid bacteria Liquid Fertilizer viable bacteria content of prepared completion.
4) by the lactic acid bacteria liquid fertilizer of preparation it is aseptic subpackaged, storage.
Embodiment 2：
The present embodiment, is made of the raw material of following parts by weight (kg)：Amino-acid liquid fertilizer 40kg, lactic acid bacteria Liquid Fertilizer
20kg, above-mentioned amino-acid liquid fertilizer 40kg, lactic acid bacteria Liquid Fertilizer 20kg are mixed for (25 DEG C) in room temperature according to parts by weight
It is even, you can.Mixed liquor is sterile, packing；Prepared with regard to completing a kind of organic-biological compounding alkaline land soil improvement fertilizer.
A kind of amino-acid liquid fertilizer described in the present embodiment, is made of the raw material of following parts by weight (kg)：
2000 parts of the feather suspension of uniform state；3942 20 parts of neutral proteinases (vigor 200000U/g)；1398 is neutral
30 parts of protease (vigor 30000U/g)；166 12.5 parts of unwrapping wire mycoproteinases (vigor 30000U/g)；5 parts of pancreatin.Urea 12.5
Part, 7.5 parts of sodium hydrogensulfite, manganese chloride 5.
Preparation method is identical according to the operation of parts by weight of raw materials proportioning and embodiment 1.
Lactic acid bacteria Liquid Fertilizer described in the present embodiment, is made of the raw material of following parts by weight (kg)：By weight will be edible
5 parts of white granulated sugar, edible 5 parts of brown sugar, 2.5 parts of analysis for soybean powder, 2 parts of xanthans, 22 parts of converted starch, 1 part of sucrose ester (SE), high temperature ɑ-
0.01 part of starch liquefacation enzyme, 0.003 part of sodium pyrophosphate, 0.02 part of dipotassium hydrogen phosphate, above-mentioned material is mixed, adds purified water 91,
0.02 part of lactobacillus bulgaricus, 0.02 part of streptococcus thermophilus.
Preparation method is same as Example 1 according to parts by weight of raw materials proportioning operation.
Embodiment 3：
A kind of organic-biological compounding liquid fertilizer of the present embodiment, is made of the raw material of following parts by weight (kg)：
Amino-acid liquid fertilizer 30kg, lactic acid bacteria Liquid Fertilizer 40kg are matched according to parts by weight of raw materials and operated：At room temperature (25 DEG C)
It is mixed evenly, you can.Mixed liquor is sterile, packing；With regard to completing a kind of organic-biological compounding alkaline land soil improvement liquid
It is prepared by fertilizer.
Amino-acid liquid fertilizer, is made of the raw material of following parts by weight (kg)：1000 parts of the feather suspension of uniform state；
3942 15 parts of neutral proteinases (vigor 200000U/g)；1398 25 parts of neutral proteinases (vigor 30000U/g)；166 actinomyces
18 parts of protease (vigor 30000U/g)；6 parts of pancreatin；25 parts of urea；8 parts of sodium hydrogensulfite；Manganese chloride 9.
Preparation method is identical according to the operation of parts by weight of raw materials proportioning and embodiment 1.
Lactic acid bacteria Liquid Fertilizer, is made of the raw material of following parts by weight (kg)：By weight by edible 5 parts of white granulated sugar, eat
5 parts of brown sugar, 2.5 parts of analysis for soybean powder, 2 parts of xanthans, 22 parts of converted starch, 1 part of sucrose ester (SE), high temperature ɑ-starch liquefacation enzyme 0.01
Part, 0.003 part of sodium pyrophosphate, 0.02 part of dipotassium hydrogen phosphate, above-mentioned material is mixed, adds purified water 91, lactobacillus bulgaricus
0.02 part, 0.02 part of streptococcus thermophilus.
Preparation method is same as Example 1 according to parts by weight of raw materials proportioning operation.
Embodiment 4：Application process is tested：
First, test objective
Exist to explore muskmelon at salt-soda soil (pH value 7.54) using organic-biological compounding alkaline land soil improvement liquid fertilizer
Muskmelon Growth rule, Resistant expression and yield result, design and implement this experiment, are salt-soda soil muskmelon green non-pollution
And the production that organises provides scientific basis.
2nd, experimental design
It is using great Qu contrast tests, i.e., whole to apply organic-biological compounding alkaline land soil improvement liquid fertilizer (following letter
Liquid fertilizer) it is referred to as processing, whole process applies chemical fertilizer as control (ck).Fertilization structure two handle respectively with liquid fertilizer and chemical fertilizer carry out base fertilizer and
The administration of top dressing.Handle dose such as table one.The base fertilizer of chemical fertilizer processing (ck) and top dressing are compound fertilizer (N：P：K=22：
11：12).The base fertilizer of liquid fertilizer processing and top dressing are liquid fertilizer.Great Qu areas are 667m2；If three repetitions.
One test process dose of table
Unit：Kg/acre
3rd, material to be tested：
For trying melon variety：Radix curcumae is fragrant, and liquid fertilizer compounds alkaline land soil for organic-biological and improves liquid fertilizer, and compound fertilizer is
The general goods fertilizer of in the market purchase.
4th, for trying field basic condition：
It is located at 2 groups of Xi'an Yanliang District frontier passes and mountains town Shui Zhai villages for examination field；Soil types is grey soil property hilltop soil.Quality is low
Earth, soil fertility are low etc., pH value 7.54；Preceding stubble is corn, 464 kilograms of per unit area yield；Irrigation condition is medium, exchange facility.
5th, organic-biological compounding alkaline land soil improvement liquid fertilizer and influence of the common chemical fertilizer to Muskmelon Plants character：
(being shown in Table 2)
2 liquid fertilizer of table is tested with chemical fertilizer contrast Muskmelon Plants character
6th, organic-biological compounding alkaline land soil improvement liquid fertilizer and influence of the common chemical fertilizer to melon fruit character：
(being shown in Table 3)
3 liquid fertilizer of table is tested with chemical fertilizer contrast melon fruit character
Seven, organic-biologicals compounding alkaline land soil improvement liquid fertilizer and influence of the common chemical fertilizer to melon fruit quality：
(being shown in Table 4)
4 liquid fertilizer of table is tested with chemical fertilizer contrast melon fruit quality
8th, result of the test economic characters and Yield comparison (being shown in Table 5)：
Table 5, different disposal muskmelon economic characters and Yield comparison (3 repetition average value)
9th, result of the test resistance comparative analysis (being shown in Table six)
The whole observation of muskmelon breeding time and the degeneration-resistant sex expression of the processing of investigation two, key resistance list 6.It was found from table six, have
Machine biology compounding alkaline land soil improvement liquid fertilizer processing is conducive to improve muskmelon comprehensive resistance, final directly or indirectly to influence
To muskmelon fruiting rate and single melon weight, the Economic Characters of table five also demonstrate this point.
6 resistance of table compares
Unit：%
Tenth, discuss
1st, this experiment shows, in low fertility or medium field, cultivates muskmelon, mu applies organic-biological compounding alkaline land soil
100 kilograms of liquid fertilizer is improved, is fully able to meet the needs of muskmelon is to three big nutrients, realizes volume increase.
2 organic-biologicals compounding alkaline land soil improvement liquid fertilizer, nutrient are complete, and especially the content of organic matter is high, and speed is slow
Have both lasting period length, will not both cause muskmelon to overgrow, de- fertile early ageing will not be caused.
3rd, for opposite chemical fertilizer, organic-biological compounding alkaline land soil improvement liquid fertilizer fertilizer efficiency is relatively slow, and making top dressing should use up
May early apply, i.e., under being applied before the appearance of muskmelon summit of growth, would rather the seedling such as fertilizer, can not the fertilizer such as seedling, to meet muskmelon normal growth
Demand.
11, summarize：
The comparative experiments of organic-biological compounding alkaline land soil improvement liquid fertilizer and common chemical fertilizer is from muskmelon in plant property
Shape (table 1), fruit properties (table 3), fruit quality (table 4), economic characters and yield (table 5), resistance (table 6) aspect：Muskmelon
Organic-biological compounding alkaline land soil improvement liquid fertilizer is applied in plantation and shows preferable Comprehensive Traits.It is in particular in：
Growth potential is strong, yield is high, fruit glossiness is good, fruit shape is beautiful, disease resistance is strong, thin skin meat is thick, sugariness is high, fragranced is dense.Thus
Illustrate that organic-biological compounding alkaline land soil improvement liquid fertilizer is applied in fruits and vegetables produce reduces chemical substance to farmland ring
The pollution in border, has saved investment of production, may advantageously facilitate green, organic farm products production, while improve alkaline land soil simultaneously
Crops resistance is improved, optimizes quality.Therefore, Devoting Major Efforts To Developing organic-biological compounding alkaline land soil improvement Liquid Fertilizer
Material, is development sustainable agriculture and circulation, the important step of sustainable agriculture.
Claims (1)
1. application of a kind of organic-biological compounding liquid fertilizer in alkaline land soil improvement, it is characterised in that according to raw material
Parts by weight, take 1-50 parts of amino-acid liquid fertilizer, and 1-50 parts of lactic acid bacteria Liquid Fertilizer is mixed evenly in room temperature；The aminoacids solution
The amino acid contained total amount 200-220g/L of body fertilizer；1,000,000,000/mL of lactic acid bacteria Liquid Fertilizer viable bacteria content；
The amino-acid liquid fertilizer, weight share composition：Take 1000~3000 parts of the feather suspension of uniform state；In 3942
5~30 parts of protease of property；1398 5~30 parts of neutral proteinases；166 5~30 parts of unwrapping wire mycoproteinases；2~10 parts of pancreatin；Urea
5~30 parts；1-10 parts of sodium hydrogensulfite；1~10 part of manganese chloride；
The lactic acid bacteria Liquid Fertilizer, raw material are according to parts by weight composition：Edible 1-10 parts of white granulated sugar, eats 1-10 parts of brown sugar, yellow
1-10 parts of bean powder, 1-3 parts of xanthans, 1-3 parts of converted starch, 1-2 parts of sucrose ester, high temperature ɑ -0.01-0.03 parts of starch liquefacation enzyme,
0.001-0.003 parts of sodium pyrophosphate, 0.01-0.05 parts of dipotassium hydrogen phosphate, above-mentioned material is mixed, and adds 73-95 parts of purified water, is protected
Add 0.01-0.02 parts of Leah lactobacillus, 0.01-0.02 parts of streptococcus thermophilus；
Prepared by following steps：
A., poultry feather is prepared into the feather suspension of uniform state；
B. urea, sodium hydrogensulfite, pancreatin in the feather suspension placing response kettle prepared, will be sequentially added, maintains 180
Turn/the mixing speed of min stirring 2h, and be warming up to 45-47 DEG C, add 3942 neutral proteinases and 1398 neutral proteinases, dimension
Suspension is made in addition 166 unwrapping wire mycoproteinase of enzyme preparation after holding the mixing speed 12h of 180 turns/min, uses 30% hydroxide
Calcium adjusts pH value to 6.8-7.2, and 1.1 × 105~1.1 × 110After continuing enzyme hydrolysis 18h under Pa pressure, manganese chloride is added,
With 12mol/L sulphur acid for adjusting pH value to 5.0,76-80 DEG C of insulation 5h is heated to, the filtering of 600 mesh of pressure glass fibre filter bag machine, is received
The filter residue collected is rich in amino acid and various trace elements and organic matter, is the green organic fertilizer of vegetables, flowers, the filter of collection
Liquid is exactly enzyme hydrolysis poultry feather Amino acid liquid fertilizer, aseptic subpackaged storage, the Amino acid liquid fertilizer institute of prepared completion
200-220g/L containing total amino acid content；
The feather suspension is prepared as：
1) poultry feather is collected, through impurity elimination, cleaning, stops clearly to water, place at aeration-drying and receive solar irradiation and dry, its
After aqueous 8-10%, then place in baking oven and dried, drying temperature is set in 47-52 DEG C and obtains dry feather；
2) the drying feather of preparation is placed to the sheet that 1-3mm is ground into pulverizer, example in mass ratio prepares mixed liquor, powder
Yu Mao ︰ distilled water is 1 ︰ 5, and the mixed liquor after compounding is placed on to the sheet mixed liquor that 0.02-0.03mm is ground into colloid mill,
Sheet mixed liquor is placed to the feather suspension that uniform state is prepared into homogeneous machine again；
The lactic acid bacteria Liquid Fertilizer produces the lactic acid bacteria liquid fertilizer according to the following steps：
1) by weight by edible white granulated sugar 1-10 parts, 1-10 parts of brown sugar is eaten, 1-10 parts of analysis for soybean powder, 1-3 parts of xanthans, becomes
1-3 parts of starch of property, 1-2 parts of sucrose ester, high temperature ɑ -0.01-0.03 parts of starch liquefacation enzyme, 0.001-0.003 parts of sodium pyrophosphate, phosphorus
Sour 0.01-0.05 parts of hydrogen dipotassium, above-mentioned material is mixed, and is added 73-95 parts of purified water to place in homogeneous machine and is prepared into uniform shape
The suspension of state, then it is used as culture medium after carrying out high-temperature sterilization processing；The high-temperature process is kept at a temperature of 110-130 DEG C
30-120min sterilization times；
2) combination of both following strains is inoculated with when liquid medium being cooled to 35-40 DEG C, according to parts by weight, inoculation measures 0.02-
0.04 part；The strain is 0.01-0.02 parts of lactobacillus bulgaricus, 0.01-0.02 parts of streptococcus thermophilus；
3) the inoculation mixed liquor of step (2) is placed in culture tank, when keeping 35-38 DEG C of culture 48 small, pH value reaches 3.3-4.5
When, glass cloth filtering, collects the preparation that filtrate completes lactic acid bacteria liquid fertilizer；
4) by the lactic acid bacteria liquid fertilizer of preparation it is aseptic subpackaged, storage.
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