CN116814459A - Microorganism strain with functions of inducing cucumber to promote growth and resisting salt and alkali and application formula thereof - Google Patents
Microorganism strain with functions of inducing cucumber to promote growth and resisting salt and alkali and application formula thereof Download PDFInfo
- Publication number
- CN116814459A CN116814459A CN202310000324.4A CN202310000324A CN116814459A CN 116814459 A CN116814459 A CN 116814459A CN 202310000324 A CN202310000324 A CN 202310000324A CN 116814459 A CN116814459 A CN 116814459A
- Authority
- CN
- China
- Prior art keywords
- cucumber
- strain
- growth
- alkali
- inducing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 title claims abstract description 83
- 230000012010 growth Effects 0.000 title claims abstract description 54
- 239000003513 alkali Substances 0.000 title claims abstract description 42
- 230000001939 inductive effect Effects 0.000 title claims abstract description 27
- 150000003839 salts Chemical class 0.000 title claims abstract description 18
- 244000005700 microbiome Species 0.000 title claims description 11
- 244000299906 Cucumis sativus var. sativus Species 0.000 title 1
- 240000008067 Cucumis sativus Species 0.000 claims abstract description 83
- 238000000034 method Methods 0.000 claims abstract description 38
- 238000003973 irrigation Methods 0.000 claims abstract description 24
- 230000002262 irrigation Effects 0.000 claims abstract description 24
- 230000000813 microbial effect Effects 0.000 claims abstract description 22
- 230000008569 process Effects 0.000 claims abstract description 22
- 239000012224 working solution Substances 0.000 claims abstract description 22
- BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 claims abstract description 18
- 150000001875 compounds Chemical class 0.000 claims abstract description 17
- 230000001737 promoting effect Effects 0.000 claims abstract description 15
- 239000002689 soil Substances 0.000 claims abstract description 14
- 238000004321 preservation Methods 0.000 claims abstract description 10
- OGNSCSPNOLGXSM-UHFFFAOYSA-N (+/-)-DABA Natural products NCCC(N)C(O)=O OGNSCSPNOLGXSM-UHFFFAOYSA-N 0.000 claims abstract description 9
- 229960003692 gamma aminobutyric acid Drugs 0.000 claims abstract description 9
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 7
- 238000009629 microbiological culture Methods 0.000 claims abstract description 5
- 241000315694 Bacillus tequilensis Species 0.000 claims abstract 4
- 230000001580 bacterial effect Effects 0.000 claims description 20
- 239000007788 liquid Substances 0.000 claims description 16
- 235000013350 formula milk Nutrition 0.000 claims description 15
- 239000000843 powder Substances 0.000 claims description 10
- 238000005728 strengthening Methods 0.000 claims description 10
- 241000196324 Embryophyta Species 0.000 claims description 7
- 235000013399 edible fruits Nutrition 0.000 claims description 6
- 238000009331 sowing Methods 0.000 claims description 6
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 claims description 6
- 241000894006 Bacteria Species 0.000 claims description 5
- 238000013329 compounding Methods 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 4
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 3
- 229930006000 Sucrose Natural products 0.000 claims description 3
- 239000007853 buffer solution Substances 0.000 claims description 3
- 239000005018 casein Substances 0.000 claims description 3
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 claims description 3
- 235000021240 caseins Nutrition 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims description 3
- 229910052700 potassium Inorganic materials 0.000 claims description 3
- 239000011591 potassium Substances 0.000 claims description 3
- 238000005507 spraying Methods 0.000 claims description 3
- 239000004575 stone Substances 0.000 claims description 3
- 239000000758 substrate Substances 0.000 claims description 3
- 239000005909 Kieselgur Substances 0.000 claims description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 2
- 238000009472 formulation Methods 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 235000020610 powder formula Nutrition 0.000 claims description 2
- 239000000725 suspension Substances 0.000 claims description 2
- 230000000844 anti-bacterial effect Effects 0.000 claims 2
- 239000003899 bactericide agent Substances 0.000 claims 2
- 238000012258 culturing Methods 0.000 claims 1
- 238000001035 drying Methods 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 3
- 239000011780 sodium chloride Substances 0.000 abstract 3
- 235000013311 vegetables Nutrition 0.000 description 9
- 241000193830 Bacillus <bacterium> Species 0.000 description 6
- 230000000694 effects Effects 0.000 description 3
- 230000015784 hyperosmotic salinity response Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 230000004913 activation Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- LJRGBERXYNQPJI-UHFFFAOYSA-M sodium;3-nitrobenzenesulfonate Chemical compound [Na+].[O-][N+](=O)C1=CC=CC(S([O-])(=O)=O)=C1 LJRGBERXYNQPJI-UHFFFAOYSA-M 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/05—Fruit crops, e.g. strawberries, tomatoes or cucumbers
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical & Material Sciences (AREA)
- Environmental Sciences (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Pest Control & Pesticides (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Virology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Forests & Forestry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Dentistry (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Agronomy & Crop Science (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a microbial strain with functions of inducing cucumber growth and resisting salt and alkali and an application formula thereof, belongs to the technical field of cultivation and application of microbial strains, and relates to a microbial strain with functions of inducing cucumber growth and resisting salt and alkali and an application formula thereof, wherein the microbial strain is Bacillus tequilensis (deposited in the common microorganism center of China general microbiological culture preservation management committee, the preservation date is 2022 and 9 and 19 days, the preservation number is CGMCC No. 25754), the quality of the seedlings can be obviously improved by applying the strain to a conventional seedling culture or factory seedling culture process in a mode of a strain growth promoting working solution or a compound microbial inoculum, and the saline and alkali resistance of the cucumber can be also induced by root irrigation application after the strain is compounded with gamma-aminobutyric acid to form a saline and alkali compound resistance inducing strain working solution in a process of planting cucumber in saline and alkali soil, so that the cucumber production benefit is effectively improved.
Description
Technical Field
The invention relates to the technical field of cultivation and application of microbial strains, in particular to a microbial strain with functions of inducing cucumber to promote growth and resisting salt and alkali and an application formula thereof.
Background
Vegetables occupy important positions in agricultural production and resident consumption in China, and are crops planted in the second largest class in China. The growth promotion, yield increase and quality improvement are three fundamental targets of high-quality and high-efficiency cultivation of vegetables, and effective measures for realizing standardized, intensive and modern vegetable production are key links for realizing high-quality vegetable production and improving vegetable yield and quality. In the past researches on promoting the growth of vegetables and improving the yield of vegetables, chemical fertilizers or chemical regulating substances are mainly focused on, but in recent years, the beneficial effects of rhizosphere growth promoting bacteria on the processes of organic nutrient conversion and crop element absorption are gradually emphasized, and functional microorganism strains are mined, so that the method is a novel technical means for realizing 'crop growth promotion-soil protection-food safety'. Saline-alkali soil is a general term for various saline-alkali soil and alkaline soil with different degrees of salinization and alkalization, and is characterized in that the soil contains more saline-alkali components, so that the growth of most plants is inhibited with different degrees. The measures for improving the salt tolerance of vegetables are significant in improving the yield, improving the quality and improving the economic benefit of vegetables, microorganisms are used as indigenous organisms in soil, and are nontoxic, harmless and environment-friendly, so that the attention of countries and scientific researchers is increasingly paid in recent years, and the efficient microorganism strain can promote plant growth, can help to improve the activity of the soil, improve the soil environment and restore the soil fertility level.
In view of the above, the invention provides a microorganism strain with functions of inducing cucumber to promote growth and resisting salt and alkali, and develops an application formula thereof.
Disclosure of Invention
1. Technical problem to be solved
Aiming at the problems existing in the prior art, the invention aims to provide a microorganism strain with functions of inducing cucumber to promote growth and resisting salt and alkali and an application formula thereof, which can be added into a matrix under the condition of normal seedling growth or in the process of industrial seedling raising to obviously accelerate the growth speed of seedlings and improve the seedling raising quality, and when cucumber is cultivated in saline-alkali soil or saline-alkali water source area, a composite strain working solution containing gamma-aminobutyric acid (GABA) is added into root irrigation of rhizosphere to obviously induce cucumber seedlings to resist salt stress.
2. Technical proposal
In order to solve the problems, the invention adopts the following technical scheme.
A microbial strain with functions of inducing cucumber to promote growth and resisting salt and alkali, wherein the microbial strain is bacillus tertagatous (Bacillus equilensis SX 31) and is preserved in China general microbiological culture Collection center (China Committee for culture Collection), the preservation date is 2022, 9 months and 19 days, the preservation number is CGMCC No.25754, and the preservation address is: beijing, china.
Furthermore, in the process of being applied to the cucumber growth promotion and seedling strengthening, the application method for promoting the cucumber seedling growth is that the bacterial liquid is subjected to root irrigation in a resuspension manner or is formed into compound bacterial agent powder for application.
Furthermore, in the process of being applied to cucumber growth promotion and seedling strengthening, the bacterial liquid re-suspension root irrigation application formula comprises the following steps: the bacillus equisetis sx31 strain was subjected to 2 activation cultures to a concentration of 1 x 10 8 ～1.0*10 9 CFU/mL, after centrifugation, was concentrated to a concentration of 1.0 x 10 by re-suspending with 0.1mol/L, ph=7.0, in PBS buffer solution 9 ～1.0*10 11 CFU/mL is the strain growth promoting working solution.
Further, in the process of applying the strain to cucumber growth promotion and seedling strengthening, the root irrigation dosage of the strain growth promotion working solution for promoting cucumber seedling growth according to the cucumber seedling growth stage division is as follows:
root irrigation dosage during cucumber sowing: 1*10 8 ～5*10 9 CFU/strain;
root irrigation dosage when the true leaves of the cucumber are flattened to two leaves and one heart: 5*10 10 ～1*10 11 CFU/strain;
root irrigation dose after cucumber three leaves one heart (or after field planting): 5*10 11 CFU/strain.
Further, in the process of being applied to the cucumber growth promotion and seedling strengthening, the process of forming the compound microbial inoculum powder formula comprises the following components in parts by weight: potassium fulvate: casein: diatomaceous earth: stone powder: white sugar = 1:4:6:3:2, the compounding method is that bacterial strains are attached to a carrier by using a bacterial liquid spraying mode in a constant temperature sterile stirring process at 28 ℃, and then stirred, enriched and cultured in a sterile environment at 28 ℃ and dried until the effective viable bacteria concentration is 2 x 10 10 CFU/g。
Further, in the process of being applied to cucumber growth promotion and seedling strengthening, the application dosage and the method of the compound microbial inoculum powder are as follows:
compounding seedling substrate during cucumber sowing: 0.5 g/strain;
the cucumber is planted from two leaves to three leaves and one heart: 1-3 g/strain.
Furthermore, when the application method is applied to inducing the salt-tolerant alkalinity of the cucumber, the application method is to form a saline-alkali compound induced-resistance strain working solution.
Further, when the strain working solution is applied to inducing salt tolerance alkaline of cucumber, the concentration of the strain working solution is adjusted to be 1.0 x 10 9 CFU/mL, and then gamma-aminobutyric acid (GABA) is added to a final concentration of 10-50 mmol/L, which is the saline-alkali compound induced resistance strain working solution.
Furthermore, when the method is applied to inducing salt-tolerant alkalinity of the cucumber, after the cucumber suffers from saline-alkali stress or when the cucumber is cultivated in saline-alkali soil, the dosage of the saline-alkali composite induced resistance strain working solution is as follows according to the growth stage of the cucumber seedling:
the root irrigation dose before three leaves and one heart of cucumber is as follows: 10-20 mL/plant;
the root irrigation dosage of the cucumber from three leaves to one heart to before flowering and fruit setting is as follows: 50-100 mL/plant;
after flowering and fruit setting of cucumber: 150-200 mL/strain.
3. Advantageous effects
Compared with the prior art, the invention has the advantages that:
the scheme provides a microorganism strain with functions of inducing cucumber growth promotion and salt tolerance and an application formula thereof, the strain can realize the cultivation effects of promoting growth, strengthening seedlings and increasing yield in the conventional cucumber seedling raising or cultivation production process according to the scheme, and the strain can obviously induce cucumber plants to resist salt stress in the process of cultivating the cucumber in saline-alkali soil or saline-alkali water source irrigation land according to the scheme, so that the cultivation purposes of saving cost and improving efficiency are realized.
Drawings
FIG. 1 is a comparative Table I of the present invention after treatment with a control CK-C and BacillusequiumsisX 31 bacterial solution;
FIG. 2 is a comparative Table II of the present invention after treatment with a control CK-C and Bacillus equisetis SX31 bacterial liquid.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention; it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments, and that all other embodiments obtained by persons of ordinary skill in the art without making creative efforts based on the embodiments in the present invention are within the protection scope of the present invention.
In the description of the present invention, it should be noted that the positional or positional relationship indicated by the terms such as "upper", "lower", "inner", "outer", "top/bottom", etc. are based on the positional or positional relationship shown in the drawings, are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the apparatus or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "configured to," "engaged with," "connected to," and the like are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
Example 1:
referring to fig. 1-2, a microorganism strain with functions of inducing cucumber to promote growth and resisting salt and alkali is bacillus tertag, which is preserved in China general microbiological culture Collection center (China general microbiological culture Collection center), with a preservation date of 2022, 9 months and 19 days, and a preservation number of CGMCC No.25754.
The application method for promoting the growth of cucumber seedlings is that bacterial liquid is re-suspended and root-irrigated or compounded to form bacterial agent powder.
The bacterial liquid resuspension root irrigation application formula comprises the following steps: the bacillus equisetis sx31 strain was subjected to 2 activation cultures to a concentration of 1 x 10 8 ～1.0*10 9 CFU/mL, after centrifugation, was concentrated to a concentration of 1.0 x 10 by re-suspending with 0.1mol/L, ph=7.0, in PBS buffer solution 9 ～1.0*10 11 CFU/mL is the working solution of the growth-promoting bacterial liquid.
The root irrigation dosage of the growth promoting bacterial liquid working solution for promoting the growth of cucumber seedlings according to the growth stage division of the cucumber seedlings is as follows:
root irrigation dosage during cucumber sowing: 1*10 8 ～5*10 9 CFU/strain;
root irrigation dosage when the true leaves of the cucumber are flattened to two leaves and one heart: 5*10 10 ～1*10 11 CFU/strain;
root irrigation dose after cucumber three leaves one heart (or after field planting): 5*10 11 CFU/strain.
In the process of forming the compound microbial inoculant powder formulation, the carrier comprises the following components in parts by weight: potassium fulvate: casein: diatom diatomSoil: stone powder: white sugar = 1:4:6:3:2, the compounding method is that bacterial strains are attached to a carrier by using a bacterial liquid spraying mode in a constant temperature sterile stirring process at 28 ℃, and then stirred, enriched and cultured in a sterile environment at 28 ℃ and dried until the effective viable bacteria concentration is 2 x 10 10 CFU/g。
Example 2:
the application dosage and the method of the compound microbial inoculum powder are as follows:
compounding seedling substrate during cucumber sowing: 0.5 g/strain;
the cucumber is planted from two leaves, one heart to three leaves, one heart: 1-3 g/strain.
The concentration of the working solution of the bacterial liquid is adjusted to be 1.0-10 9 And adding gamma-aminobutyric acid (GABA) into CFU/mL to a final concentration of 30-100 mmol/L, wherein the CFU/mL is the saline-alkali induced-resistance type bacterial liquid working solution.
After the cucumber is subjected to saline-alkali stress, the saline-alkali induced resistance type bacterial liquid working solution for inducing the cucumber to resist the saline-alkali is divided according to the growth stage of the cucumber seedling, and the root filling dosage is as follows: :
the root irrigation dose before three leaves and one heart of cucumber is as follows: 10-20 mL/plant;
the root irrigation dosage from three leaves of cucumber to one heart (or after field planting) before flowering and fruit setting is as follows: 50-100 mL/plant;
after flowering and fruit setting of cucumber: 150-200 mL/strain.
The above description is only of the preferred embodiments of the present invention; the scope of the invention is not limited in this respect. Any person skilled in the art, within the technical scope of the present disclosure, may apply to the present invention, and the technical solution and the improvement thereof are all covered by the protection scope of the present invention.
Claims (9)
1. A microbial strain with functions of inducing cucumber to promote growth and resisting salt and alkali is characterized in that: the microbial strain is Bacillus tequilensis (Bacillus tequilensis SX), and is preserved in China general microbiological culture Collection center (China Committee for culture Collection of microorganisms) with a preservation date of 2022, 9 months and 19 days and a preservation number of CGMCC No.25754.
2. The application formula of the microbial strain with functions of inducing cucumber growth and resisting salt and alkali according to claim 1, which is characterized in that: in the process of applying the compound bactericide to the cucumber seedling, the application method for promoting the cucumber seedling growth is to apply the bacterium liquid to resuspension and irrigate roots or to apply the compound bactericide powder.
3. The application formula of the microbial strain with functions of inducing cucumber growth and resisting salt and alkali according to claim 2, which is characterized in that: in the process of applying the bacterial liquid to cucumber growth promotion and seedling strengthening, the bacterial liquid re-suspension root irrigation application formula comprises the following steps: bacillus tequilensis SX31 strain was grown 2 times to a concentration of 1 x 10 8 ～1.0*10 9 CFU/mL, after centrifugation, was concentrated to a concentration of 1.0 x 10 by re-suspending with 0.1mol/L, ph=7.0, in PBS buffer solution 9 ～1.0*10 11 CFU/mL is the strain growth promoting working solution.
4. The formulation for applying a microorganism strain having both functions of inducing cucumber growth and resisting salt and alkali according to claim 3, wherein: in the process of applying the strain growth promoting working solution to the cucumber seedling growth promotion and strengthening process, the strain growth promoting working solution for promoting the cucumber seedling growth is divided according to the cucumber seedling growth stage, and the root filling dosage is as follows:
root irrigation dosage during cucumber sowing: 1*10 8 ～5*10 9 CFU/strain;
root irrigation dosage when the true leaves of the cucumber are flattened to two leaves and one heart: 5*10 10 ～1*10 11 CFU/strain;
root irrigation dose after cucumber three leaves one heart (or after field planting): 5*10 11 CFU/strain.
5. The microbial strain with functions of inducing cucumber to promote growth and resisting salt and alkali according to claim 2, wherein the microbial strain and an application formula thereof are characterized in that: in the process of being applied to the cucumber growth promotion and seedling strengthening, the process of forming the compound microbial inoculum powder formula comprises the following components in parts by weight: potassium fulvate: casein: diatomaceous earth: stone powder: white sugar = 1:4:6:3:2, its compoundingThe method comprises the steps of attaching a strain to a carrier by using a bacterial liquid spraying mode in a constant-temperature aseptic stirring process at 28 ℃, stirring, enriching and culturing in an aseptic environment at 28 ℃ and drying until the effective viable bacteria concentration is 2 x 10 10 CFU/g。
6. The microbial strain with functions of inducing cucumber growth and resisting salt and alkali according to claim 5, wherein the microbial strain and an application formula thereof are characterized in that: in the process of applying the compound microbial inoculum to cucumber growth promotion and seedling strengthening, the application dosage and the method of the compound microbial inoculum powder are as follows:
compounding seedling substrate during cucumber sowing: 0.5 g/strain;
the cucumber is planted from two leaves to three leaves and one heart: 1-3 g/strain.
7. The application formula of the microbial strain with functions of inducing cucumber growth and resisting salt and alkali according to claim 1, which is characterized in that: when the application method is applied to inducing the salt-tolerant alkalinity of the cucumber, the application method is to form the saline-alkali compound induced-resistance strain working solution.
8. The microbial strain with functions of inducing cucumber growth and resisting salt and alkali according to claim 7, wherein the microbial strain and an application formula thereof are characterized in that: when applied to inducing salt-tolerant alkalinity of cucumber, the concentration of the working solution of the strain in claim 3 is adjusted to be 1.0 x 10 9 CFU/mL, and then gamma-aminobutyric acid (GABA) is added to a final concentration of 10-50 mmol/L, which is the saline-alkali compound induced resistance strain working solution.
9. The microbial strain having both functions of inducing cucumber growth and resisting salt and alkali according to claim 7 and claim 8, and an application formula thereof, wherein the microbial strain is characterized in that: when the method is applied to inducing salt-tolerant alkalinity of the cucumber, after the cucumber suffers from saline-alkali stress or when the cucumber is cultivated in saline-alkali soil, the dosage of the saline-alkali compound induced strain working solution is as follows:
the root irrigation dose before three leaves and one heart of cucumber is as follows: 10-20 mL/plant;
the root irrigation dosage of the cucumber from three leaves to one heart to before flowering and fruit setting is as follows: 50-100 mL/plant;
after flowering and fruit setting of cucumber: 150-200 mL/strain.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310000324.4A CN116814459A (en) | 2023-01-02 | 2023-01-02 | Microorganism strain with functions of inducing cucumber to promote growth and resisting salt and alkali and application formula thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310000324.4A CN116814459A (en) | 2023-01-02 | 2023-01-02 | Microorganism strain with functions of inducing cucumber to promote growth and resisting salt and alkali and application formula thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116814459A true CN116814459A (en) | 2023-09-29 |
Family
ID=88141859
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310000324.4A Pending CN116814459A (en) | 2023-01-02 | 2023-01-02 | Microorganism strain with functions of inducing cucumber to promote growth and resisting salt and alkali and application formula thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116814459A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117603886A (en) * | 2024-01-18 | 2024-02-27 | 东北农业大学 | Saline-alkali resistant rhizosphere growth promoting microbial agent and preparation method and application thereof |
-
2023
- 2023-01-02 CN CN202310000324.4A patent/CN116814459A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117603886A (en) * | 2024-01-18 | 2024-02-27 | 东北农业大学 | Saline-alkali resistant rhizosphere growth promoting microbial agent and preparation method and application thereof |
| CN117603886B (en) * | 2024-01-18 | 2024-04-05 | 东北农业大学 | Saline-alkali resistant rhizosphere growth promoting microbial agent and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| HU230555B1 (en) | Environment-friend micro-organism produce and producing thereof | |
| CN101671633B (en) | Antagonistic bacteria for preventing and eliminating greensickness of continuous cropping cotton and microbial organic fertilizer thereof | |
| CN101671205B (en) | Composite microbial preparation for promoting growth of rape and preparation method thereof | |
| CN110616172B (en) | Bacillus with crucifer clubroot prevention and treatment function and preparation method and application thereof | |
| CN101948780B (en) | Antagonist bacterium for preventing and treating continuous cropping hot pepper epidemic disease and microbial organic fertilizer thereof | |
| CN115340968B (en) | New application of pseudomonas microphylla and method thereof, pseudomonas microphylla 21.1.9.2-14 and products thereof | |
| CN102674986A (en) | Microbial organic fertilizer with moisture and fertility retention functions and preparation method of microbial organic fertilizer | |
| CN107580818A (en) | A kind of soil conditioning and the integrated approach repaired | |
| CN110373363A (en) | A kind of concentration microbial inoculum of bacillusmusilaginosiengineering and its preparation method and application | |
| CN116814459A (en) | Microorganism strain with functions of inducing cucumber to promote growth and resisting salt and alkali and application formula thereof | |
| CN111670769A (en) | Method for improving stress resistance of rice | |
| CN109055274B (en) | Caragana rhizobium and fermentation culture method and application thereof | |
| CN107628894A (en) | Composite bacteria agent increase soil fertility and its preparation method and application | |
| CN108048360B (en) | Bacillus subtilis with dual functions of degrading organic phosphorus and preventing diseases | |
| CN106396931A (en) | Compound nitrogen-fixing microorganism bacterium fertilizer and preparation method thereof | |
| CN102276337A (en) | Re-cultivation long-acting bio-organic fertilizer | |
| CN110713953B (en) | Mesorhizobium strain with phosphate solubilizing property and application thereof | |
| CN104988094A (en) | Method for manufacturing quinclorac solid degrading inoculant | |
| CN110218657B (en) | Trichoderma longibrachiatum MD30 and biological organic fertilizer developed by same | |
| CN102219567A (en) | Method for producing biological organic fertilizer by using methane liquid as basic culture medium through fermentation | |
| CN113957011B (en) | Agricultural liquid microbial agent and production method thereof | |
| WO1996034840A1 (en) | Bacterial fertilizer and production process | |
| CN102776139A (en) | Bacterium agent capable of dissolving phosphorus and releasing potassium and preparation method thereof | |
| CN115557815A (en) | Ginger compound microbial fertilizer capable of solving continuous cropping obstacle and preparation method thereof | |
| CN112979391B (en) | Root-promoting bio-organic fertilizer and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |