CN107455605A - A kind of feed compound for being used to reduce cholesterol level of vitellus - Google Patents
A kind of feed compound for being used to reduce cholesterol level of vitellus Download PDFInfo
- Publication number
- CN107455605A CN107455605A CN201710861242.3A CN201710861242A CN107455605A CN 107455605 A CN107455605 A CN 107455605A CN 201710861242 A CN201710861242 A CN 201710861242A CN 107455605 A CN107455605 A CN 107455605A
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- Prior art keywords
- fermentation
- feed
- lactobacillus
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- round
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- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 title claims abstract description 54
- 150000001875 compounds Chemical class 0.000 title claims abstract description 23
- 238000000855 fermentation Methods 0.000 claims abstract description 41
- 230000004151 fermentation Effects 0.000 claims abstract description 41
- 241001474374 Blennius Species 0.000 claims abstract description 22
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 claims abstract description 7
- 229910052804 chromium Inorganic materials 0.000 claims abstract description 7
- 239000011651 chromium Substances 0.000 claims abstract description 7
- 239000000203 mixture Substances 0.000 claims abstract description 6
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000011777 magnesium Substances 0.000 claims abstract description 5
- 229910052749 magnesium Inorganic materials 0.000 claims abstract description 5
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims abstract description 3
- 239000011575 calcium Substances 0.000 claims abstract description 3
- 229910052791 calcium Inorganic materials 0.000 claims abstract description 3
- 229930003231 vitamin Natural products 0.000 claims abstract description 3
- 235000013343 vitamin Nutrition 0.000 claims abstract description 3
- 239000011782 vitamin Substances 0.000 claims abstract description 3
- 229940088594 vitamin Drugs 0.000 claims abstract description 3
- 150000003722 vitamin derivatives Chemical class 0.000 claims abstract description 3
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 31
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 31
- 244000199885 Lactobacillus bulgaricus Species 0.000 claims description 31
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 claims description 31
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 31
- 229940004208 lactobacillus bulgaricus Drugs 0.000 claims description 31
- 238000002360 preparation method Methods 0.000 claims description 21
- 230000004913 activation Effects 0.000 claims description 16
- 235000015097 nutrients Nutrition 0.000 claims description 16
- 239000012530 fluid Substances 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 238000011081 inoculation Methods 0.000 claims description 8
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 7
- 238000011084 recovery Methods 0.000 claims description 7
- 238000007789 sealing Methods 0.000 claims description 7
- 241000894006 Bacteria Species 0.000 claims description 6
- 102000002322 Egg Proteins Human genes 0.000 claims description 5
- 108010000912 Egg Proteins Proteins 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 5
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 4
- CPLXHLVBOLITMK-UHFFFAOYSA-N Magnesium oxide Chemical compound [Mg]=O CPLXHLVBOLITMK-UHFFFAOYSA-N 0.000 claims description 4
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 claims description 4
- LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 claims description 4
- 241000276489 Merlangius merlangus Species 0.000 claims description 3
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims description 2
- 229930003451 Vitamin B1 Natural products 0.000 claims description 2
- 229930003268 Vitamin C Natural products 0.000 claims description 2
- 229930003448 Vitamin K Natural products 0.000 claims description 2
- 229940046374 chromium picolinate Drugs 0.000 claims description 2
- GJYSUGXFENSLOO-UHFFFAOYSA-N chromium;pyridine-2-carboxylic acid Chemical compound [Cr].OC(=O)C1=CC=CC=N1.OC(=O)C1=CC=CC=N1.OC(=O)C1=CC=CC=N1 GJYSUGXFENSLOO-UHFFFAOYSA-N 0.000 claims description 2
- 210000003278 egg shell Anatomy 0.000 claims description 2
- 239000000395 magnesium oxide Substances 0.000 claims description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 2
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 2
- 235000012054 meals Nutrition 0.000 claims description 2
- SHUZOJHMOBOZST-UHFFFAOYSA-N phylloquinone Natural products CC(C)CCCCC(C)CCC(C)CCCC(=CCC1=C(C)C(=O)c2ccccc2C1=O)C SHUZOJHMOBOZST-UHFFFAOYSA-N 0.000 claims description 2
- RADKZDMFGJYCBB-UHFFFAOYSA-N pyridoxal hydrochloride Natural products CC1=NC=C(CO)C(C=O)=C1O RADKZDMFGJYCBB-UHFFFAOYSA-N 0.000 claims description 2
- 229960003495 thiamine Drugs 0.000 claims description 2
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 claims description 2
- 235000010374 vitamin B1 Nutrition 0.000 claims description 2
- 239000011691 vitamin B1 Substances 0.000 claims description 2
- 235000019158 vitamin B6 Nutrition 0.000 claims description 2
- 239000011726 vitamin B6 Substances 0.000 claims description 2
- 235000019154 vitamin C Nutrition 0.000 claims description 2
- 239000011718 vitamin C Substances 0.000 claims description 2
- 235000019168 vitamin K Nutrition 0.000 claims description 2
- 239000011712 vitamin K Substances 0.000 claims description 2
- 150000003721 vitamin K derivatives Chemical class 0.000 claims description 2
- 229940011671 vitamin b6 Drugs 0.000 claims description 2
- 229940046010 vitamin k Drugs 0.000 claims description 2
- 210000000481 breast Anatomy 0.000 claims 2
- 241000193830 Bacillus <bacterium> Species 0.000 claims 1
- 235000012000 cholesterol Nutrition 0.000 abstract description 14
- 235000013601 eggs Nutrition 0.000 abstract description 13
- 239000000843 powder Substances 0.000 abstract description 10
- 240000008042 Zea mays Species 0.000 abstract description 6
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 abstract description 6
- 235000002017 Zea mays subsp mays Nutrition 0.000 abstract description 6
- 235000005822 corn Nutrition 0.000 abstract description 6
- 244000046052 Phaseolus vulgaris Species 0.000 abstract description 5
- 235000010627 Phaseolus vulgaris Nutrition 0.000 abstract description 5
- 235000002566 Capsicum Nutrition 0.000 abstract description 4
- 239000006002 Pepper Substances 0.000 abstract description 4
- 235000016761 Piper aduncum Nutrition 0.000 abstract description 4
- 235000017804 Piper guineense Nutrition 0.000 abstract description 4
- 244000203593 Piper nigrum Species 0.000 abstract description 4
- 235000008184 Piper nigrum Nutrition 0.000 abstract description 4
- 239000000796 flavoring agent Substances 0.000 abstract description 4
- 235000019634 flavors Nutrition 0.000 abstract description 4
- 239000000341 volatile oil Substances 0.000 abstract description 4
- 241000195493 Cryptophyta Species 0.000 abstract description 2
- 244000269722 Thea sinensis Species 0.000 abstract description 2
- 235000019789 appetite Nutrition 0.000 abstract description 2
- 230000036528 appetite Effects 0.000 abstract description 2
- 125000003118 aryl group Chemical group 0.000 abstract description 2
- 235000009569 green tea Nutrition 0.000 abstract description 2
- 230000002195 synergetic effect Effects 0.000 abstract description 2
- 239000002131 composite material Substances 0.000 abstract 1
- 238000009472 formulation Methods 0.000 abstract 1
- 239000000306 component Substances 0.000 description 10
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 9
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 8
- 239000007788 liquid Substances 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 230000001954 sterilising effect Effects 0.000 description 7
- 239000003795 chemical substances by application Substances 0.000 description 6
- 239000008103 glucose Substances 0.000 description 6
- 241000235342 Saccharomycetes Species 0.000 description 5
- 235000015278 beef Nutrition 0.000 description 5
- 229940041514 candida albicans extract Drugs 0.000 description 5
- YXVFQADLFFNVDS-UHFFFAOYSA-N diammonium citrate Chemical compound [NH4+].[NH4+].[O-]C(=O)CC(O)(C(=O)O)CC([O-])=O YXVFQADLFFNVDS-UHFFFAOYSA-N 0.000 description 5
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 5
- 239000012153 distilled water Substances 0.000 description 5
- 210000002969 egg yolk Anatomy 0.000 description 5
- 235000013312 flour Nutrition 0.000 description 5
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 5
- 239000006872 mrs medium Substances 0.000 description 5
- 235000016709 nutrition Nutrition 0.000 description 5
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 5
- 229920000053 polysorbate 80 Polymers 0.000 description 5
- 239000012138 yeast extract Substances 0.000 description 5
- 229920001817 Agar Polymers 0.000 description 4
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 4
- 239000008272 agar Substances 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 229910052564 epsomite Inorganic materials 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 238000002386 leaching Methods 0.000 description 4
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 238000009928 pasteurization Methods 0.000 description 4
- 108010009004 proteose-peptone Proteins 0.000 description 4
- 239000001632 sodium acetate Substances 0.000 description 4
- 235000017281 sodium acetate Nutrition 0.000 description 4
- 206010012601 diabetes mellitus Diseases 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000002054 inoculum Substances 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 201000001320 Atherosclerosis Diseases 0.000 description 2
- 241000195649 Chlorella <Chlorellales> Species 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 241001261505 Undaria Species 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 229910000019 calcium carbonate Inorganic materials 0.000 description 2
- 239000013530 defoamer Substances 0.000 description 2
- KCIDZIIHRGYJAE-YGFYJFDDSA-L dipotassium;[(2r,3r,4s,5r,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] phosphate Chemical compound [K+].[K+].OC[C@H]1O[C@H](OP([O-])([O-])=O)[C@H](O)[C@@H](O)[C@H]1O KCIDZIIHRGYJAE-YGFYJFDDSA-L 0.000 description 2
- 235000013345 egg yolk Nutrition 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 235000021323 fish oil Nutrition 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 125000004435 hydrogen atom Chemical class [H]* 0.000 description 2
- 235000021388 linseed oil Nutrition 0.000 description 2
- 239000000944 linseed oil Substances 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 238000011218 seed culture Methods 0.000 description 2
- DYNFCHNNOHNJFG-UHFFFAOYSA-M 2-formylbenzoate Chemical compound [O-]C(=O)C1=CC=CC=C1C=O DYNFCHNNOHNJFG-UHFFFAOYSA-M 0.000 description 1
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 241000186000 Bifidobacterium Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 208000035150 Hypercholesterolemia Diseases 0.000 description 1
- 208000031226 Hyperlipidaemia Diseases 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 101000905241 Mus musculus Heart- and neural crest derivatives-expressed protein 1 Proteins 0.000 description 1
- 101001031591 Mus musculus Heart- and neural crest derivatives-expressed protein 2 Proteins 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 241000195474 Sargassum Species 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- FUFJGUQYACFECW-UHFFFAOYSA-L calcium hydrogenphosphate Chemical compound [Ca+2].OP([O-])([O-])=O FUFJGUQYACFECW-UHFFFAOYSA-L 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 230000002490 cerebral effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- WORJEOGGNQDSOE-UHFFFAOYSA-N chloroform;methanol Chemical compound OC.ClC(Cl)Cl WORJEOGGNQDSOE-UHFFFAOYSA-N 0.000 description 1
- 229940125782 compound 2 Drugs 0.000 description 1
- 229940126214 compound 3 Drugs 0.000 description 1
- 230000009514 concussion Effects 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000019700 dicalcium phosphate Nutrition 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000014103 egg white Nutrition 0.000 description 1
- 210000000969 egg white Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000003031 feeding effect Effects 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 235000004213 low-fat Nutrition 0.000 description 1
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 description 1
- 229940099596 manganese sulfate Drugs 0.000 description 1
- 239000011702 manganese sulphate Substances 0.000 description 1
- 235000007079 manganese sulphate Nutrition 0.000 description 1
- CDUFCUKTJFSWPL-UHFFFAOYSA-L manganese(II) sulfate tetrahydrate Chemical compound O.O.O.O.[Mn+2].[O-]S([O-])(=O)=O CDUFCUKTJFSWPL-UHFFFAOYSA-L 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 230000000474 nursing effect Effects 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- AICOOMRHRUFYCM-ZRRPKQBOSA-N oxazine, 1 Chemical compound C([C@@H]1[C@H](C(C[C@]2(C)[C@@H]([C@H](C)N(C)C)[C@H](O)C[C@]21C)=O)CC1=CC2)C[C@H]1[C@@]1(C)[C@H]2N=C(C(C)C)OC1 AICOOMRHRUFYCM-ZRRPKQBOSA-N 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- 238000010079 rubber tapping Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 208000019553 vascular disease Diseases 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Animal Husbandry (AREA)
- Biotechnology (AREA)
- Birds (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Sustainable Development (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Fodder In General (AREA)
Abstract
The present invention relates to a kind of feed compound for being used to reduce cholesterol level of vitellus.The compound includes following composition by weight ratio：5 20 parts of fermentation of seaweed thing；46 parts of green tea powder；13 parts of Japan pepper essential oil；Organic 12 parts of trivalent chromium；25 parts of vitamin；5 10 parts of calcium source feed；12 parts of magnesium source feed；50 60 parts of corn；20 30 parts of dregs of beans.The feed compound of the present invention with the addition of algae component, Japan pepper essential oil so that feed flavour is delicious, aromatic flavour, can promote the appetite of laying hen.The composite formulations are reasonable, can significantly reduce cholesterol content in eggs, improve laying rate, have synergy and synergistic effect well.
Description
Technical field
The present invention relates to a kind of feed compound, more particularly to a kind of feed for being used to reduce cholesterol level of vitellus to answer
Compound.
Background technology
In recent years, with the continuous improvement greatly developed with living standards of the people of China's economy, person in middle and old age's population ratio
Gradually increase, and suffer from the ratio of the diseases such as hypercholesterolemia, hyperlipidemia, hypertension, atherosclerosis and diabetes
Substantially increase.Weighed very much so active prevention and early treatment diabetes, the angiocarpy and prognosis for diabetic have
The influence wanted, it is of crucial importance actively to find a kind of high nutrition, the dietary product of low fat.
Egg is a kind of food that people often eat, and especially in the north, egg is that essential on dining table one is beautiful
Food.But due in yolk cholesterol it is high, eat that egg is excessive, can greatly increase the intake of cholesterol, cause cholesterolemia
Too high levels, cause the generation of atherosclerosis and Cardial or cerebral vascular diseases.So many people due to cholesterol not
Dare to eat egg, the people particularly to suffer from a heart complaint dare not more eat yolk, only eat egg white.
Existing patent CN103689267A discloses a kind of high DHA low cholesterol eggs nutritional agents and production method, the battalion
Supporting agent includes mixing the fermentation of seaweed product that marine alga is fermented to obtain with fish oil, linseed oil.But its effect needs into one
Step improves.
How more effectively to reduce Cholesterol Content in Egg Yolk becomes urgent problem to be solved.
The content of the invention
(1) technical problems to be solved
In order to solve the above mentioned problem of prior art, the present invention provides a kind of feeding for being used to reduce cholesterol level of vitellus
Expect compound.
The present invention still further provides the preparation method of above-mentioned feed compound.
(2) technical scheme
In order to achieve the above object, the main technical schemes that the present invention uses include：
A kind of feed compound for being used to reduce cholesterol level of vitellus, including following composition by weight ratio：
Preferably, a kind of feed compound for being used to reduce cholesterol level of vitellus, including following composition by weight ratio：
Wherein, fermentation of seaweed thing is prepared by the following method：
S1, inoculation：By lactobacillus bulgaricus (Lactobacillus bulgaricus) and lactobacillus acidophilus
The activation culture thing of (Lactobacillus acidophilus) is inoculated into round, adds water；
S2, sealing：The marine alga of pretreatment is put into round, sealed；
S3, fermentation：Round is fermented 4-12 days at 20 DEG C -30 DEG C, obtains fermentation of seaweed thing.
Further, above-mentioned fermentate can be dried, and be comprised the following steps：By fermentate low temperature drying after fermentation ends,
It is ground into fine powder.
Preferably, in step S1, the activation of lactobacillus bulgaricus includes：Under aseptic condition, by lactobacillus bulgaricus
It is inoculated in MRS fluid nutrient mediums, at 40 DEG C -45 DEG C, 150-200rpm, recovery culture 16-24h；The activation bag of lactobacillus acidophilus
Include：Lactobacillus acidophilus is inoculated in MRS fluid nutrient mediums, at 36 DEG C -38 DEG C, 150-200rpm, recovery culture 18-24h.
Preferably, in step S1, the mass ratio of lactobacillus bulgaricus and lactobacillus acidophilus is 3:1, activation culture thing is pressed
Volume ratio 5-20% is inoculated into round.
Preferably, in step S2, the pretreatment of marine alga includes cleaning marine alga, is filtered dry, and crushes, and crosses 100 mesh sieves.
Wherein, organic trivalent chromium includes chromium picolinate and/or yeast chromium.
Wherein, vitamin includes the one or more in vitamin K, vitamin B1, vitamin B6, vitamin C.
Wherein, calcium source feed includes the one or more in agstone, oyster shell whiting, egg-shell meal.
Wherein, magnesium source feed includes the one or more in magnesia, magnesium sulfate, Magnesium Asparticum.
The present invention also provides a kind of preparation of fermentation of seaweed thing in feed compound for reducing cholesterol level of vitellus
Method, comprise the following steps：
S1, inoculation：By lactobacillus bulgaricus (Lactobacillus bulgaricus) and lactobacillus acidophilus
The activation culture thing of (Lactobacillus acidophilus) is inoculated into round, adds water；
S2, sealing：The marine alga of pretreatment is put into round, sealed；
S3, fermentation：Round is fermented 4-12 days at 20 DEG C -30 DEG C, obtains fermentation of seaweed thing.
Wherein, in step S1, the activation of lactobacillus bulgaricus includes：Under aseptic condition, lactobacillus bulgaricus is connect
Kind is in MRS fluid nutrient mediums, at 40 DEG C -45 DEG C, 150-200rpm, recovery culture 16-24h；The activation bag of lactobacillus acidophilus
Include：Lactobacillus acidophilus is inoculated in MRS fluid nutrient mediums, at 36 DEG C -38 DEG C, 150-200rpm, recovery culture 18-24h.
Wherein, in step S1, the mass ratio of lactobacillus bulgaricus and lactobacillus acidophilus is 3:1, activation culture thing presses body
Product is inoculated into round than 5-20%.In step S2, the pretreatment of marine alga includes cleaning marine alga, is filtered dry, and crushes, mistake
100 mesh sieves.
Present invention also offers a kind of preparation method of feed addictive, each components in certain proportion is well mixed, produced.
(3) beneficial effect
The beneficial effects of the invention are as follows：(1) feed addictive of the invention can significantly reduce cholesterol content in eggs,
Improve laying rate.(2) feed addictive of the invention with the addition of algae component, Japan pepper essential oil, and its flavour is delicious, aromatic flavour,
It can improve a poor appetite.(3) feed addictive prescription of the invention is reasonable, has synergy and synergistic effect well.
Embodiment
In order to preferably explain the present invention, in order to understand, by embodiment, the present invention is described in detail.
Bacterium source：Lactobacillus bulgaricus (Lactobacillus bulgaricus) and lactobacillus acidophilus
(Lactobacillus acidophilus) is common strain, can be commercially available by commercial sources.In the embodiment of the present invention
Lactobacillus bulgaricus is purchased from Chinese agriculture Culture Collection, and lactobacillus acidophilus is purchased from Chinese agriculture microorganism fungus kind
Collection.Japan pepper essential oil is purchased from fragrant (Hancheng) Chinese prickly ash Co., Ltd long.Green tea powder is purchased from vegetable agricultural Co., Ltd in Jiangsu.
Embodiment 1
The preparation of fermentation of seaweed thing
By lactobacillus bulgaricus, with MRS culture mediums, the 150-200rpm at 40 DEG C cultivates 16-24h, by lactobacillus acidophilus
With MRS culture mediums, 150-200rpm cultivates 18-24h at 36 DEG C.Each bacterium bacterium number is set to respectively reach 108Individual -1010Individual/g culture mediums.
Wherein, the MRS medium components：Peptone 10g, yeast extract 5g, beef extract 10g, glucose 20g, second
Sour sodium 5g, dibasic ammonium citrate 2g, Tween-80 1mL, MgSO4.7H2O 0.58g, MnSO4.4H2O 0.05g, K2HPO42g, fine jade
Cosmetics 17g, water 1000mL, adjust pH6.2-6.4,121 DEG C, 1 × 105Pa sterilizings 20min.
By lactobacillus bulgaricus nutrient solution and lactobacillus acidophilus nutrient solution by weight 3:1 ratio mixing, is configured to
Leavening.
Sea-tangle is cleaned, is filtered dry, it is broken after, cross 100 mesh sieves, pass through pasteurization before fermentation.
The preparation method of fermentation of seaweed thing：
S1, inoculation：Above-mentioned leavening is inoculated in 500L rounds for 5% by volume, adds water, to fermentation
At the 2/3 of vessel volume；
S2, sealing：Above-mentioned sea-tangle is put into round so that zymotic fluid identity distance from vessel port 1-2cm at, cover
Lid seals；
S3, fermentation：Round is fermented 12 days at 20 DEG C, detection pH value is 5, obtains fermentation of seaweed thing 1.
Embodiment 2
The preparation of fermentation of seaweed thing
By lactobacillus bulgaricus, with MRS culture mediums, the 150-200rpm at 45 DEG C cultivates 16-24h, by lactobacillus acidophilus
With MRS culture mediums, 150-200rpm cultivates 18-24h at 38 DEG C.Each bacterium bacterium number is set to respectively reach 108Individual -1010Individual/g culture mediums.
Wherein, lactobacillus bulgaricus MRS medium components：Casein peptone 10g, yeast extract 5g, beef leaching
Thing 10g, glucose 20g, sodium acetate 5g, dibasic ammonium citrate 2g, Tween 80 1g, K2HPO42g, MgSO4·7H2O 0.2g,
MnSO4·7H2O 0.05g, agar powder 20g, distilled water 1L, adjust pH6.2-6.4,121 DEG C, 1 × 105Pa sterilizings 20min.
Lactobacillus acidophilus MRS medium components：Casein peptone 10g, yeast extract 5g, beef leaching thing 10g, Portugal
Grape sugar 20g, sodium acetate 5g, dibasic ammonium citrate 2g, Tween 80 1g, K2HPO42g, MgSO4·7H2O 0.2g, MnSO4·7H2O
0.05g, CaCO320g, agar powder 20g, distilled water 1L, adjust pH6.2-6.4,121 DEG C, 1 × 105Pa sterilizings 20min.
By lactobacillus bulgaricus nutrient solution and lactobacillus acidophilus nutrient solution by weight 3:1 ratio mixing, is configured to
Leavening.
Sargassum is cleaned, is filtered dry, it is broken after, cross 100 mesh sieves, pass through pasteurization before fermentation.
The preparation method of fermentation of seaweed thing：
S1, inoculation：Above-mentioned leavening is inoculated in 500L rounds for 20% by volume, adds water, to fermentation
At the 2/3 of vessel volume；
S2, sealing：Above-mentioned chlorella is put into round so that zymotic fluid identity distance from vessel port 1-2cm at, lid
Upper cover seals；
S3, fermentation：Round is fermented 4 days at 30 DEG C, detection pH value is 3, obtains fermentation of seaweed thing 2.
Embodiment 3
The preparation of fermentation of seaweed thing
By lactobacillus bulgaricus, with MRS culture mediums, the 150-200rpm at 42 DEG C cultivates 16-24h, by lactobacillus acidophilus
With MRS culture mediums, 150-200rpm cultivates 18-24h at 37 DEG C.Each bacterium bacterium number is set to respectively reach 108Individual -1010Individual/g culture mediums.
Wherein, lactobacillus bulgaricus MRS medium components：Casein peptone 10g, yeast extract 5g, beef leaching
Thing 10g, glucose 20g, sodium acetate 5g, dibasic ammonium citrate 2g, Tween 80 1g, K2HPO42g, MgSO4·7H2O 0.2g,
MnSO4·7H2O 0.05g, agar powder 20g, distilled water 1L, adjust pH6.2-6.4,121 DEG C, 1 × 105Pa sterilizings 20min.
Lactobacillus acidophilus MRS medium components：Casein peptone 10g, yeast extract 5g, beef leaching thing 10g, Portugal
Grape sugar 20g, sodium acetate 5g, dibasic ammonium citrate 2g, Tween 80 1g, K2HPO42g, MgSO4·7H2O 0.2g, MnSO4·7H2O
0.05g, CaCO320g, agar powder 20g, distilled water 1L, adjust pH6.2-6.4,121 DEG C, 1 × 105Pa sterilizings 20min.
By lactobacillus bulgaricus nutrient solution and lactobacillus acidophilus nutrient solution by weight 3:1 ratio mixing, is configured to
Leavening.
Undaria pinnitafida is cleaned, is filtered dry, it is broken after, cross 100 mesh sieves, pass through pasteurization before fermentation.
The preparation method of fermentation of seaweed thing：
S1, inoculation：Above-mentioned leavening is inoculated in 500L rounds for 15% by volume, adds water, to fermentation
At the 2/3 of vessel volume；
S2, sealing：Above-mentioned chlorella is put into round so that zymotic fluid identity distance from vessel port 1-2cm at, lid
Upper cover seals；
S3, fermentation：Round is fermented 8 days at 25 DEG C, detection pH value is 4, obtains fermentation of seaweed thing 3.
Embodiment 4
The preparation of feed compound
Formula：
Above-mentioned formula components are mixed to stir evenly and produce feed compound 1.
Embodiment 5
The preparation of feed compound
Formula：
Above-mentioned formula components are mixed to stir evenly and produce feed compound 2.
Embodiment 6
The preparation of feed compound
Formula：
Above-mentioned formula components are mixed to stir evenly and produce feed compound 3.
Test example (prepares feed) with reference to embodiment 3 in patent CN103689267A
The preparation of tunning：
(1) shake-flask seed liquid is prepared：Using saccharomycete, bacillus subtilis, Bifidobacterium as starting strain, inoculum concentration ratio
Example is 3:1:1, under aseptic condition, bacterium germination will be gone out and be inoculated in fluid nutrient medium, cultivated 17h, obtain shake-flask seed liquid；
(2) saccharomycete seed liquor is prepared：Shake-flask seed liquid is by volume with 4% inoculum concentration, aseptically, inoculation
In 50L seeding tanks, 15h is cultivated, fermentation obtains saccharomycete seed liquor；
(3) fermented and cultured：The saccharomycete seed liquor that step 2 is obtained, it is inoculated in fermentation tank, ferments with 10% by volume
Period adds marine alga frequently, and the addition of marine alga is the 15% of fermentation cumulative volume, cultivates 25h, stops tank tapping, obtain tunning.
Marine alga is undaria pinnitafida, is 40 mesh powder, passes through pasteurization before fermentation.
In step (1), inoculum concentration is the 5% of fluid nutrient medium volume, and shake-flask seed culture medium formula is：Glucose
0.5%, 20 mesh corn flour 1.0%, dipotassium hydrogen phosphate 0.3%, 20%NaOH solution regulation pH=7.5, shake-flask seed culture bar
Part is：35 DEG C, 150rpm, liquid amount 180mL of temperature；
In step (2), culture medium prescription is：Glucose 0.5%, 20 mesh corn flour 1.0%, 20 mesh bean cake powders 3%, phosphorus
Sour hydrogen dipotassium 0.3%, defoamer 0.07%, 20%NaOH solution regulation pH=7.5；Saccharomycete seed liquor condition of culture is：Temperature
37 DEG C, 220rpm, liquid amount 40L of degree, throughput 2.2L/h；
In step (3), culture medium prescription is glucose 0.5%, 20 mesh corn flour 1.0%, 20 mesh bean cake powders 3%, phosphoric acid
Hydrogen dipotassium 0.3%, manganese sulfate 0.05%, defoamer 0.07%, 20%NaOH solution regulation pH=7.5；Fermentation condition is：Temperature
35 DEG C, 120rpm, liquid amount 400L of degree, throughput 2.5L/h；
The culture medium passes through moist heat sterilization, and sterilising conditions are 121 DEG C, autoclaving 20-30min.
The preparation of nutritional agents：By weight percentage, by fermentation of seaweed product 30% and fish oil 20%, linseed oil 50%
Well mixed obtained nutritional agents.
The feed formula be corn flour 60%, dregs of beans 30%, oyster shell whiting 6%, calcium monohydrogen phosphate 1%, retinol1 %,
Mineral matter iron 1%, nutritional agents 1%.
Control group 1：
With embodiment 6, difference is to be not added with fermentation of seaweed thing preparation method.
Control group 2：
With embodiment 6, difference is to be not added with yeast chromium preparation method.
Control group 3：
With embodiment 6, difference is to be not added with fermentation of seaweed thing and yeast chromium preparation method.
Control group 4：
Feed formula：Corn flour 70%, dregs of beans 30%.
Laying hen feeding effect is tested and assessed
1st, animal experiment
Experiment is carried out in certain chicken farm.Laying hen 240 is chosen, is divided into 1-8 groups, every group 30, according to embodiment 4, implements
Feed prepared by example 5, embodiment 6, control group 1, control group 2, control group 3, control group 4, test example is successively to 1-8 group laying hens
Fed, nursing time 30 days, gather egg daily.
2nd, the content of cholesterol in egg is detected
Using OPA development process, cholesterol detection content.Specific method is：
Agents useful for same：OPA, 90% acetic acid, mixed acid：The isometric concentrated sulfuric acid+isometric 90% acetic acid,
0.1mg/ml standard cholesterol applications liquid, calf serum
(1) drafting of standard curve：
Take 5 test tubes, be separately added into 0,0.1,0.2,0.3,0.4mL standard cholesterol application liquid, sequentially add acetic acid
It is 0.4mL to make each pipe volume.Often pipe adds O-phthalic aldehyde reagent 0.2mL, distilled water 0.01mL, adds 4mL mixed acid,
Concussion shakes up.10min determines light absorption value after 550nm.Using cholesterol level mg/100ml as abscissa, absorbance value is vertical
Coordinate draws standard curve.
(2) preparation of sample：8 test tubes are taken, numbering 1-8, with methanol chloroform solution, extract the egg in 1-8 groups successively
Cholesterol in yolk, determine cholesterol level.Every piece of cholesterol content in eggs=cholesterol weight/egg yolk weight, experiment
Carry out three times, every group of 10 eggs every time, taking average.
(3) measure of laying rate
Laying rate=(the egg sum/laying hen number of one day) × 100%, takes average.
(4) testing result：As shown in table 1
The testing result of table 1
From table 1, embodiment 4-6 cholesterol level is significantly lower than control group and test example (P<0.05), laying rate
Apparently higher than control group and test example (P<0.05).
The technical principle of the present invention is described above in association with embodiment.These descriptions are intended merely to explain this hair
Bright principle, and limiting the scope of the invention can not be construed in any way.Based on explanation herein, this area
Technical staff, which need not pay creative work, can associate the other embodiments of the present invention, and these modes will all fall
Enter within the scope of the present invention.
Claims (10)
1. a kind of be used to reduce the feed compound of cholesterol level of vitellus, it is characterised in that including by weight ratio as
Lower composition：
2. feed compound as claimed in claim 1, it is characterised in that including following composition by weight ratio：
3. feed compound as claimed in claim 1 or 2, it is characterised in that the fermentation of seaweed thing is prepared by the following method
Obtain：
S1, inoculation：By lactobacillus bulgaricus (Lactobacillus bulgaricus) and lactobacillus acidophilus
The activation culture thing of (Lactobacillus acidophilus) is inoculated into round, adds water；
S2, sealing：The marine alga of pretreatment is put into round, sealed；
S3, fermentation：Round is fermented 4-12 days at 20 DEG C -30 DEG C, obtains fermentation of seaweed thing.
4. feed compound as claimed in claim 3, it is characterised in that in step S1, the activation bag of lactobacillus bulgaricus
Include：Under aseptic condition, lactobacillus bulgaricus is inoculated in MRS fluid nutrient mediums, at 40 DEG C -45 DEG C, 150-200rpm is multiple
Soviet Union culture 16-24h；The activation of lactobacillus acidophilus includes：Lactobacillus acidophilus is inoculated in MRS fluid nutrient mediums, 36 DEG C -38 DEG C
Under, 150-200rpm, recovery culture 18-24h.
5. feed compound as claimed in claim 3, it is characterised in that in step S1, lactobacillus bulgaricus and acidophilus breast
The mass ratio of bacillus is 3:1,5-20% is inoculated into round activation culture thing by volume.
6. feed compound as claimed in claim 3, it is characterised in that in step S2, the pretreatment of marine alga is included marine alga
Cleaning, is filtered dry, and crushes, and crosses 100 mesh sieves.
7. feed compound as claimed in claim 1 or 2, it is characterised in that organic trivalent chromium include chromium picolinate and/or
Yeast chromium.
The vitamin includes the one or more in vitamin K, vitamin B1, vitamin B6, vitamin C.
The calcium source feed includes the one or more in agstone, oyster shell whiting, egg-shell meal.
The magnesium source feed includes the one or more in magnesia, magnesium sulfate, Magnesium Asparticum.
8. the preparation method of fermentation of seaweed thing, its feature exist in a kind of feed compound for reducing cholesterol level of vitellus
In comprising the following steps：
S1, inoculation：By lactobacillus bulgaricus (Lactobacillus bulgaricus) and lactobacillus acidophilus
The activation culture thing of (Lactobacillus acidophilus) is inoculated into round, adds water；
S2, sealing：The marine alga of pretreatment is put into round, sealed；
S3, fermentation：Round is fermented 4-12 days at 20 DEG C -30 DEG C, obtains fermentation of seaweed thing.
9. preparation method as claimed in claim 8, it is characterised in that in step S1, the activation of lactobacillus bulgaricus includes：
Under aseptic condition, lactobacillus bulgaricus is inoculated in MRS fluid nutrient mediums, at 40 DEG C -45 DEG C, 150-200rpm, recovery
Cultivate 16-24h；The activation of lactobacillus acidophilus includes：Lactobacillus acidophilus is inoculated in MRS fluid nutrient mediums, 36 DEG C -38 DEG C
Under, 150-200rpm, recovery culture 18-24h.
10. preparation method as claimed in claim 8, it is characterised in that in step S1, lactobacillus bulgaricus and acidophilus breast bar
The mass ratio of bacterium is 3:1,5-20% is inoculated into round activation culture thing by volume.In step S2, the pre- place of marine alga
Reason includes cleaning marine alga, is filtered dry, and crushes, and crosses 100 mesh sieves.
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