实施例4、EeFRG1及其同源基因在新一代小麦杂交育种技术中的应用
FRG1及其同源基因可以用于新一代杂交育种技术,该技术的核心思想是:以frg1隐性核雄性不育突变体为转化受体材料,通过将紧密连锁的3个目标基因转化至不育突变体中,其中,育性恢复基因FRG1可使转化受体育性恢复,花粉失活基因可使含有外源基因的花粉失活,即失去授精能力,种子标记基因可以用于转基因种子和非转基因种子的分拣,分拣出的非转基因种子即为不育系,而转基因种子用作保持系。通过保持系给不育系授粉杂交,可以在不育系上结实,由此繁殖不育系。而保持系通过自交可以源源不断地得以繁殖。由于该技术利用生物技术生产非转基因产品,解决了小麦等作物在杂交制种过程中面临的瓶颈问题,即三系法资源利用率低而两系法中不育系育性不稳定的问题。
根据以上原理,发明人首先分别对表达载体内的ZmBT1-ZmAA、FRG1和mCherryW三个表达盒单独进行了小麦转化,并进一步对各个表达盒的功能进行了验证。结果表明各个表达盒单独转化小麦时,都能够工作良好,达到预期的设计效果。其中FRG1表达盒中只要包含的是有功能的FRG1基因即可,例如EeFRG1,TaFRG1-1,TaFRG1-2,TuFRG1,AetFRG1,SbFRG1,SiFRG1,ZmFRG1-1或ZmFRG1-2等均可恢复frg1雄性不育突变体的育性。
进一步,发明人通过装配下述DNA元件,构建了pAHC20-FRG1-AA-mCherryW载体:
1)以pAHC20载体为基础;
2)FRG1基因表达盒,有功能的FRG1基因连接于EeFRG1基因启动子(如SEQ ID NO:1)的下游、EeFRG1基因终止子(如SEQ ID NO:3)的上游,FRG1基因可以是EeFRG1,TaFRG1-1,TaFRG1-2,TuFRG1,AetFRG1,SbFRG1,SiFRG1,ZmFRG1-1或ZmFRG1-2,序列如SEQ ID NO:2,SEQ ID NO:5,SEQ ID NO:8,SEQ ID NO:11,SEQ ID NO:14,SEQ ID NO:17,SEQ ID NO:20,SEQ ID NO:23,SEQ ID NO:26所示;
3)基因表达盒PG47:ZmBT1-ZmAA-IN2-1,目标基因为ZmAA,转运肽为ZmBT1,ZmBT1-ZmAA(其核苷酸序列如SEQ ID NO:29所示)的开放读码框连接于启动子PG47(其核苷酸序列如SEQ ID NO:30所示)的下游、终止子IN2-1(其核苷酸序列如SEQ ID NO:31所示)的上游。
4)基因表达盒CaMV35S增强子-LTP2:mCherryW-PINII,mCherryW基因(SEQ ID NO:32)的开放读码框连接于CaMV35S增强子-LTP2启动子(SEQ ID NO:33)和PINII终止子(SEQID NO:34)之间,重组成mCherryW的基因表达盒(CaMV35S增强子-LTP2:mCherryW-PINII);
利用基因枪法将质粒pAHC20-FRG1-AA-mCherryW转化4Ag-ms单体异附加系植株幼胚,经过筛选、分化、壮苗生根、PCR鉴定等过程,得到基因型为兰州核不育位点纯合突变、无长穗偃麦草4Ag染色体、且转基因为单拷贝的转基因阳性植株。
对上述植株进行花粉活性检测,在非转基因植株中,不育花粉的比例小于2%,而转基因植株中不育花粉的比例为50%左右,表明本发明所提供的载体能够达到预期的花粉失活功能。
对植株所结T1代种子进行荧光分离比例调查,结果表明这些种子均显示1:1分离比,即携带外源基因的荧光种子和不携带外源基因的非荧光种子表现为1:1分离,表明本发明所提供的载体各元件作为整体表达良好,可以实现创制和繁殖不育系的目的。
SEQUENCE LISTING
<110> 未名兴旺***作物设计前沿实验室(北京)有限公司
北京大学
<120> 雄性育性的保持方法及其应用
<130>
<160> 35
<170> PatentIn version 3.3
<210> 1
<211> 2265
<212> DNA
<213> 长穗偃麦草(Elytrigia elongata)
<400> 1
gctttgccta catactctag agaagttcaa cccatgatcc ataagcaagc tctcaattgc 60
tgttttgagt gtcaaagatg tggtgtttgc aacatataaa atacgaagaa acctctcaac 120
cacccttcct attttaggta cacatcactt gattcatgga caagtattga gaaacaacta 180
tcacctagct ctttcatcat gcgcctagta gtttctttgg cacaagggtt gatgaggtat 240
gtttgtatat cttgagctat cattttattg ttccttgaag cattcttgag aaccaccttg 300
ttcacttctt cagaaatctc tactagccat tgtataagct caaggaaatt acctttatgc 360
agtgaaactt ctttccatca tgtccacgaa aagctaaccc ttgatgcaaa agaaaccttg 420
gacattaaaa ggaatatgtc aagccagcta tatataaaac cttgtcttgt ttggtggttg 480
aaaaaatgac tctatgattg ttgtttttgg tgcaatgaag aaattgtatt tctcttgtgc 540
tttgttgtgt gcactgttga ttccaccaac atgtttcaaa aaactatcct tgtgattcta 600
atttctaaat cacccgttca caaaaaatct ccacaaacat gcaaattgtc cttgaaaaga 660
taacatacaa gtagaaagca tcatcttttt tactatactc aagccaacta taaggcttaa 720
accatttagc tacaaatatc gatgcacacc tccggtgggg tgttgtggaa aagcatgtta 780
ttttggccga taagcccctt ttacagtgta tcctcttcta attctattca gatcattaac 840
atcagctgtg attgacatcc tcttcccaag attagattca cgcaattgaa catcataaac 900
cacatcttca atgtcatcct cttcctatat attttcagat tattggcttg cttcgttctc 960
aatatcaggt tctatgaatg gacttctgtt gatgccacta ataatttgta gttgttgtgg 1020
aatataaatt gaacgggagc tcatggtgct atgaacttga ttggatgaga aattgttcta 1080
cagctccact tgctgctcaa cctaaataca tgcttggatt tcttcccagc tctagcacat 1140
aaagttttca aattaatgtt tccaccacat aaagttttga aatccacaaa tacttttttt 1200
agtacatgaa catttttcta atatacggtg aagatttttc atatacaaac tgatcgtttt 1260
aatatatggt aaaaattggt gtaatatatg ctgaaatgtt ttcaaataca tattgaatat 1320
atttataata aatggtgagc atttctgtaa tacatgatga ccattttaaa aatacatatc 1380
gaacatttca taatatacga tgaacagttt tataatactc gatgaacata ttttggagtt 1440
ctgaacaatt ttttttcaaa aacacaagcc attttccagg aaaaaacaaa agcaaaagaa 1500
atgagaaacc caaaaaacaa aaaagcaaag cagaacaaaa caaaacaaaa caaaacagag 1560
aaatctacac ggaaaatgaa acagaaaaag taaagaaaga acccgaactg ggccaggcaa 1620
tgtttgcaac ggcctcgctc ttcctgaaca agaaggtcag tccatgggct gcttccagta 1680
cacgggcctc gctgtgtggc aacacgccat gtaatagttt tcgcgggaat ccaacgtcgg 1740
tctggtgact tctggtgcct accagaactc tccacaagct cccacaggac ccacgcagcc 1800
gtctgatcag atcagcacga agcacgaact ttgaagcgcg gcgatatttt ctctccccag 1860
cctccgcctc gcccgacgac gctgcgctgc agcaccattt caatttcaaa atcaaaatcg 1920
aaaacggaaa agctttctcg catcccgagg agaggcggtt acgcgcgcca gaggagcacg 1980
agagaggcca ccccacccac ccccgccctc acgtgccgcg ctcgcacccc ccgcggccgc 2040
atccgggccg tccgcgcgga cagctggccg cgccccaccc gaaccgacgc ccaggatcga 2100
gcgagagcgg cgcgcccgag gcttggctta gcgtccacgg ctcctccggc tatataaccc 2160
gcccgccacc cgctccccct ccggcattcc attccgccac cgcaccccca ctccaccaca 2220
aaccctagcg agcgagcgag agggagagac cgccccgccc cgccg 2265
<210> 2
<211> 1878
<212> DNA
<213> 长穗偃麦草(Elytrigia elongata)
<400> 2
atggagagat cccggcgcct gctgctcgtg gcgggcctgc tggcggcgct gctcccggcg 60
gcggcggccg cgttcgggcc gcagccgggg gcgccgtgcg agcccacgct gctggcgacg 120
caggtggcgc tcttctgcgc gcccgacatg cccaccgcgc agtgctgcga gcccgtcgtc 180
gccgccgtcg acctcggcgg cggcgtcccc tgcctctgcc gcgtcgccgc ggagccgcag 240
ctcgtcatgg cgggcctcaa cgccacccac ctcctcacgc tctacagctc ctgcggcggc 300
ctccgccccg gcggcgccca cctcgccgcc gcctgcgaag gtacgcaccg cacgtcgcct 360
ccttccctcc ctccctctac gtgccgattt tctgtgctcg ctttcctgct tacctagtag 420
tacgtagttt cccatggctt ctcgagtcgc tatagtgctc cgatttgggt cggtttcctc 480
gctgttctac cggatctgtc ggcacggcgc gcggcgtcgg gttctcgccg tctcccgtgg 540
cgagcgacct gcgcagcgcg cgcacggcct agctagcttc ttcataccgc tgtaccttga 600
gatatacgga gcgatttagg gtctactctg agtatttcgt catcgtagaa cgcatgtgcc 660
acgcgcgatt gtttcatcga ttctagatct gtgtttgttc ccgcgagtta agatggatct 720
agcgccgtac gcagatgcgg aggccttgcg gtctctgtag ctcgagttat cttatctact 780
gtcgttcgac tacagtattt gcctgcttcc ttttcactgg gtttatcgtg cagtagtagt 840
agccatgtcc acgccttctt gttttgaggc gatcatcgtg gacatacgtt tcctgctaca 900
gatttgagga gcactttgtt tcaaactgca acgcagcttt gctttctgca gtatcttctg 960
ccttgttttg ttctgtgcag tacctcttgc ttggtcaaaa ctgaaaacgc ttgctgtttg 1020
atcggcagag caaaagcttg ccgtgctttt cgctctgcag tgcatcgcct ctgcgtcttt 1080
tcccaaacat ttccgcgttg atcctctggc ggcactgctt ttttgcatgc ggtttccgta 1140
gccttcctat ttcgtgaaaa aaggttgggt caaatcaaat ggatcgccta ttggcagagc 1200
agcagcagca acagatagct ggctgtctcg cagctttggc agaatcggtc tgtggccatc 1260
tgtcccctgc caccgttttc ctgatatttg tttctctcat ctcatcttgc ctgccactgt 1320
gcttcttttc ttgttgcgca cgtcgtcacc tctactttct ttccagattt gtttgctttt 1380
gagatacgga cgaacggctg gtaattaact ttggttgttg ttgttactgt ggattttgga 1440
cgcaggaccc gctcccccgg ccgccgtcgt cagcagcccc ccacccccgc caccgtccgc 1500
cgcacctcgc cgcaagcagc cagcgcgtac gaaccctccc tctctctctc gcctgcatct 1560
cgctctgttt atctatcttc atatgttgat cagccttgtt tacatactga catgtgctct 1620
ggatcggttt tcgcagacga cgcaccgcca ccgccgccgt cgaccgagaa gccagcgtcc 1680
ccgccgcccc aggagcacga cggcgccgcc ccccgcgcca aggccgcccc cgcccaggcg 1740
gctacctccc cgctcgcgcc cgctgccgcc accgccccgc cgccccaggc gccgcactcc 1800
gccgcggcga cggcgtcgtc caaggcggcc ttcttcttcg tcgccacggc catgctcggc 1860
ctctacatca tcctctga 1878
<210> 3
<211> 628
<212> DNA
<213> 长穗偃麦草(Elytrigia elongata)
<400> 3
gtggccgacc ccgaggccat ggtccgtcca gttgcagtag agtgctcgtc gtcttgttcc 60
gtttcatgct tgtcgccatt tgaggttcgt ttctgcagtc cggtcgaaga agacggtgga 120
ttttgagtag tagtagtagc tatcgttggc aggagtatgg agttcatgtg tcctcggtcg 180
cctagttttg gtctcaagta gtactgtctg tccgccgtgt ttgggtgttt gcgattcctc 240
tggttagatg aaccactgct atgtgatcga tcgatatgat ctgaatggaa tggatcaagt 300
tttgcgttct gctgatgatg tgatatgctt cttcgtatat actcatgctc gacctatcta 360
tgttctccca tttgaatttg tggagcaaca gtttggcatg cttttgctct gctatgaatg 420
aatgcttctt gcatgcatct tgtctttgct taatttgaac tgtagaacgg atgcagttct 480
gatttcttca tatgcatatg ctctgtatgt gttcatctct tcgaatttat tatgtagcaa 540
cagtttgtag cttttcattc tgctatgaat gaatgcctct tgcatgttgt ctttgcttaa 600
tttgtactag cagaatgcag accttgcg 628
<210> 4
<211> 220
<212> PRT
<213> 长穗偃麦草(Elytrigia elongata)
<400> 4
Met Glu Arg Ser Arg Arg Leu Leu Leu Val Ala Gly Leu Leu Ala Ala
1 5 10 15
Leu Leu Pro Ala Ala Ala Ala Ala Phe Gly Pro Gln Pro Gly Ala Pro
20 25 30
Cys Glu Pro Thr Leu Leu Ala Thr Gln Val Ala Leu Phe Cys Ala Pro
35 40 45
Asp Met Pro Thr Ala Gln Cys Cys Glu Pro Val Val Ala Ala Val Asp
50 55 60
Leu Gly Gly Gly Val Pro Cys Leu Cys Arg Val Ala Ala Glu Pro Gln
65 70 75 80
Leu Val Met Ala Gly Leu Asn Ala Thr His Leu Leu Thr Leu Tyr Ser
85 90 95
Ser Cys Gly Gly Leu Arg Pro Gly Gly Ala His Leu Ala Ala Ala Cys
100 105 110
Glu Gly Pro Ala Pro Pro Ala Ala Val Val Ser Ser Pro Pro Pro Pro
115 120 125
Pro Pro Ser Ala Ala Pro Arg Arg Lys Gln Pro Ala His Asp Ala Pro
130 135 140
Pro Pro Pro Pro Ser Thr Glu Lys Pro Ala Ser Pro Pro Pro Gln Glu
145 150 155 160
His Asp Gly Ala Ala Pro Arg Ala Lys Ala Ala Pro Ala Gln Ala Ala
165 170 175
Thr Ser Pro Leu Ala Pro Ala Ala Ala Thr Ala Pro Pro Pro Gln Ala
180 185 190
Pro His Ser Ala Ala Ala Thr Ala Ser Ser Lys Ala Ala Phe Phe Phe
195 200 205
Val Ala Thr Ala Met Leu Gly Leu Tyr Ile Ile Leu
210 215 220
<210> 5
<211> 1857
<212> DNA
<213> 小麦(Triticum aestivuml)
<400> 5
atggagagat cccgccgcct gctgctggtg gcgggcctgc tcgccgcgct gctcccggcg 60
gcggcggccg ccttcgggca gcagccgggg gcgccgtgcg agcccacgct gctggcgacg 120
caggtggcgc tcttctgcgc gcccgacatg cccaccgcgc agtgctgcga gcccgtcgtc 180
gccgccgtcg acctcggcgg cggggtcccc tgcctctgcc gcgtcgccgc ggagccgcag 240
ctcgtcatgg cgggcctcaa cgccacccac ctcctcacgc tctacagctc ctgcggcggc 300
ctccgtcccg gcggcgccca cctcgccgcc gcctgcgaag gtacgcgcac gttcaccgcc 360
ctccgtccct ccctctctct gtctacgtgc agattttctg tgctctcttt cctgcttgcc 420
tagtacgtag tgttccatgg cctctcgggc cgctagcgct ccgatttgcg ttggtttcct 480
tgctgttctg ccggatctgt tggcacggcg cgcggcgtcg ggttctcgcc gtctcccgtg 540
gcgagcgacc tgcgcagcgc gcgcggcctg gctagcttca taccgctgta ccttgagata 600
cacggagcga tttagggtct actctgagta tttcgtcatc gtaggatgca tgtgccgctc 660
gcgattgttt catcgatttg agatctgtgc ttgttcccgc gagttaagat ggatctagcg 720
ccgtacgcag atgcagagtc tgttgctcga gttaccttat ctaccgtcgt tcgactatgg 780
tatttgcctg cttccttttg gctgggttta tcgtgcagta gtagtagaca tgtggacgcg 840
ttcttcttat tttgtgccga ccatcgtcga gatacttttc ctgctacagc gtttcatcgc 900
ctgcaccatc ccgttcgtga tagcactttt gtgtcaaacc gcaacgcagc tttgctttct 960
gcggtatctt ctgccttgtt tgtcgccttg cttggtcaaa actgagaact cttgctgttt 1020
gatcgaccga gggcagaggc agagcaagag cctgccgtgc ttttggctct gcagtgcgtc 1080
gtctctgcct cctttgccaa acatttccat gttgatcctc tgggggcact gctttttcgc 1140
atgcggtttc cgtagccttc ctctttcatg aaaaaaggtt tgggtcaaat caaatggatc 1200
gcctattggc agagcagcag cagatagctg gctgtctcac agctttggca gaatcggtct 1260
gttgcctgcc accgtgtctc ttatcttgcc tgccaccgtg tctcttttct tgttgcgcac 1320
gtcgtcacct cctcctactt cttttccagt tttgtttact tttgatgaaa tacggacgaa 1380
cggctggtaa tcattaactt tggttgctgt tgttactgtg gattttggac gcaggacccg 1440
ctcccccggc cgccgtcgtc agcagccccc cgcccccgcc accgtcgacc gcacctcgcc 1500
gcaagcagcc agcgcgtgcg tacctctccc tctcgcccgc atctcgctcc gtattaactg 1560
attgtgtctg catactgacg tgtgctttgg ctttggatct gtttcgcaga cgacgcacca 1620
ccgccgccgc cgccgtccag cgacaagccg tcgtccccgc cgccgtccca ggaacacgac 1680
ggcgccgctc cccacgccaa ggccgccccc gcccaggcgg ctacctcccc gctcgcgccc 1740
gctgctgcca tcgccccgcc gccccaggcg ccacactccg ccgcgcccac ggcgtcatcc 1800
aaggcggcct tcttcttcgt cgccacggcc atgctcggcc tctacatcat cctctga 1857
<210> 6
<211> 669
<212> DNA
<213> 小麦(Triticum aestivuml)
<400> 6
atggagagat cccgccgcct gctgctggtg gcgggcctgc tcgccgcgct gctcccggcg 60
gcggcggccg ccttcgggca gcagccgggg gcgccgtgcg agcccacgct gctggcgacg 120
caggtggcgc tcttctgcgc gcccgacatg cccaccgcgc agtgctgcga gcccgtcgtc 180
gccgccgtcg acctcggcgg cggggtcccc tgcctctgcc gcgtcgccgc ggagccgcag 240
ctcgtcatgg cgggcctcaa cgccacccac ctcctcacgc tctacagctc ctgcggcggc 300
ctccgtcccg gcggcgccca cctcgccgcc gcctgcgaag gacccgctcc cccggccgcc 360
gtcgtcagca gccccccgcc cccgccaccg tcgaccgcac ctcgccgcaa gcagccagcg 420
cacgacgcac caccgccgcc gccgccgtcc agcgacaagc cgtcgtcccc gccgccgtcc 480
caggaacacg acggcgccgc tccccacgcc aaggccgccc ccgcccaggc ggctacctcc 540
ccgctcgcgc ccgctgctgc catcgccccg ccgccccagg cgccacactc cgccgcgccc 600
acggcgtcat ccaaggcggc cttcttcttc gtcgccacgg ccatgctcgg cctctacatc 660
atcctctga 669
<210> 7
<211> 222
<212> PRT
<213> 小麦(Triticum aestivuml)
<400> 7
Met Glu Arg Ser Arg Arg Leu Leu Leu Val Ala Gly Leu Leu Ala Ala
1 5 10 15
Leu Leu Pro Ala Ala Ala Ala Ala Phe Gly Gln Gln Pro Gly Ala Pro
20 25 30
Cys Glu Pro Thr Leu Leu Ala Thr Gln Val Ala Leu Phe Cys Ala Pro
35 40 45
Asp Met Pro Thr Ala Gln Cys Cys Glu Pro Val Val Ala Ala Val Asp
50 55 60
Leu Gly Gly Gly Val Pro Cys Leu Cys Arg Val Ala Ala Glu Pro Gln
65 70 75 80
Leu Val Met Ala Gly Leu Asn Ala Thr His Leu Leu Thr Leu Tyr Ser
85 90 95
Ser Cys Gly Gly Leu Arg Pro Gly Gly Ala His Leu Ala Ala Ala Cys
100 105 110
Glu Gly Pro Ala Pro Pro Ala Ala Val Val Ser Ser Pro Pro Pro Pro
115 120 125
Pro Pro Ser Thr Ala Pro Arg Arg Lys Gln Pro Ala His Asp Ala Pro
130 135 140
Pro Pro Pro Pro Pro Ser Ser Asp Lys Pro Ser Ser Pro Pro Pro Ser
145 150 155 160
Gln Glu His Asp Gly Ala Ala Pro His Ala Lys Ala Ala Pro Ala Gln
165 170 175
Ala Ala Thr Ser Pro Leu Ala Pro Ala Ala Ala Ile Ala Pro Pro Pro
180 185 190
Gln Ala Pro His Ser Ala Ala Pro Thr Ala Ser Ser Lys Ala Ala Phe
195 200 205
Phe Phe Val Ala Thr Ala Met Leu Gly Leu Tyr Ile Ile Leu
210 215 220
<210> 8
<211> 1893
<212> DNA
<213> 小麦(Triticum aestivuml)
<400> 8
atggagagat cccgcggcct gctgctggcg gcgggcctgc tggcggcgct gctgccggcg 60
gcggcggccg cgttcgggca gcagccgggg gcgccgtgcg agcccacgct gctggcgacg 120
caggtggcgc tcttctgcgc gcccgacatg cccacggccc agtgctgcga gcccgtcgtc 180
gccgccgtcg acctcggcgg cggggtgccc tgcctctgcc gcgtcgccgc ggagccgcag 240
ctcgtcatgg cgggcctcaa cgccacccac ctcctcacgc tctacggctc ctgcggcggc 300
ctccgtcccg gcggcgccca cctcgccgcc gcctgcgaag gtacgtcgcg cacgttcacc 360
gcctccctcc ctccctcgct ctctctctct ctctctctct ctctctacgt gccgattctc 420
tgtgttcgct tccctgctta cctagcacgt agttttccat ggcttctcga ctcgctggtc 480
ctccgatttg ggtcggttaa tttcctcgct gtactaccgg atctgtcggc acggcgcgcg 540
gcgtcgggtt ctcgccgtct cccgtggcga gcgacctgcg cagcgcgcgc gcggcctagc 600
tagcttcata ccgctgtacc ttcagataca cggagcgatt tagggtctac tctgagtatt 660
tcgtcatcgt aggatgcatg tggcagtcgc gattgtttca tcgattttag atctgtgctt 720
gttcccgcga gttaagatgg atctagcgcc gtacgcagac gcagatggtc ttgctgtctc 780
tgttgctcga gttatcttat ctactgtcgt tcgagtatat ttgcctgctt ccttttgatc 840
tgtgtttatc gtgcagtagc agtagccatg tccacgcctt cttgtttcga ggcgatcatc 900
gtcgagatag cgctttgttt caaaccgcaa cgcagccttt gctttctgcg gtatcttctg 960
ccttgttttt gttctgtgca gtacgtcttg cttggtcaaa agtaaaaact cttgctgttc 1020
gatcgaccga ggcctgatgc agagcaagag ctggccgtgc ttttcgctct gcagtgcatc 1080
gcctctgcct ctttggccaa acatttccat gttgatcctc tggtgtggta ctactttttt 1140
gcatgcggtt tgcgtagcct tcctctttcg tgaaaaaagg tcgggtcgcc tattggcaga 1200
gcagcagcag cagcaacaga tagctggctg tctcgcagct ttgacagaac cggtctgtgg 1260
ccatctgtcg ccgcctgcca ccgtttccct gatgtttgtt tctctcgtct catctcgcct 1320
gccactgttt cttttcttgt tgcgcacgtc gtcacctcct cctacttttt tttccagttt 1380
tgtttacttt tgagatacgg acgaacggct ggtaattact aactttggtt gctgttgtta 1440
ctgtggattt tggacgcagg acccgctccc ccggccgcca tcgtcagcag ccccccgccc 1500
ccgccaccac cgtccgccgc acctcgccgc aagcagccag cgcgtacgaa cctctccctc 1560
cctctctctc gcctgcatct cgctctgtat tagctgattg tgtttactta ctgacgtgtg 1620
ctttggcttt ggatctgttt cgcagacgac gcaccgccgc cgccgccgcc gtctagcgag 1680
aagccgtcgt ccccgccgcc gtcccaggag cacgacggcg ccgccccccg cgccaaggcc 1740
gcgcccgccc aggcgaccac ctccccgctc gcgcccgctg ccgccatcgc cccgccgccc 1800
caggcgccac actccgcggc gcccacggcg tcgtccaagg cggccttctt cttcgtcgcc 1860
acggccatgc tcggcctcta catcatcctc tga 1893
<210> 9
<211> 672
<212> DNA
<213> 小麦(Triticum aestivuml)
<400> 9
atggagagat cccgcggcct gctgctggcg gcgggcctgc tggcggcgct gctgccggcg 60
gcggcggccg cgttcgggca gcagccgggg gcgccgtgcg agcccacgct gctggcgacg 120
caggtggcgc tcttctgcgc gcccgacatg cccacggccc agtgctgcga gcccgtcgtc 180
gccgccgtcg acctcggcgg cggggtgccc tgcctctgcc gcgtcgccgc ggagccgcag 240
ctcgtcatgg cgggcctcaa cgccacccac ctcctcacgc tctacggctc ctgcggcggc 300
ctccgtcccg gcggcgccca cctcgccgcc gcctgcgaag gacccgctcc cccggccgcc 360
atcgtcagca gccccccgcc cccgccacca ccgtccgccg cacctcgccg caagcagcca 420
gcgcacgacg caccgccgcc gccgccgccg tctagcgaga agccgtcgtc cccgccgccg 480
tcccaggagc acgacggcgc cgccccccgc gccaaggccg cgcccgccca ggcgaccacc 540
tccccgctcg cgcccgctgc cgccatcgcc ccgccgcccc aggcgccaca ctccgcggcg 600
cccacggcgt cgtccaaggc ggccttcttc ttcgtcgcca cggccatgct cggcctctac 660
atcatcctct ga 672
<210> 10
<211> 223
<212> PRT
<213> 小麦(Triticum aestivuml)
<400> 10
Met Glu Arg Ser Arg Gly Leu Leu Leu Ala Ala Gly Leu Leu Ala Ala
1 5 10 15
Leu Leu Pro Ala Ala Ala Ala Ala Phe Gly Gln Gln Pro Gly Ala Pro
20 25 30
Cys Glu Pro Thr Leu Leu Ala Thr Gln Val Ala Leu Phe Cys Ala Pro
35 40 45
Asp Met Pro Thr Ala Gln Cys Cys Glu Pro Val Val Ala Ala Val Asp
50 55 60
Leu Gly Gly Gly Val Pro Cys Leu Cys Arg Val Ala Ala Glu Pro Gln
65 70 75 80
Leu Val Met Ala Gly Leu Asn Ala Thr His Leu Leu Thr Leu Tyr Gly
85 90 95
Ser Cys Gly Gly Leu Arg Pro Gly Gly Ala His Leu Ala Ala Ala Cys
100 105 110
Glu Gly Pro Ala Pro Pro Ala Ala Ile Val Ser Ser Pro Pro Pro Pro
115 120 125
Pro Pro Pro Ser Ala Ala Pro Arg Arg Lys Gln Pro Ala His Asp Ala
130 135 140
Pro Pro Pro Pro Pro Pro Ser Ser Glu Lys Pro Ser Ser Pro Pro Pro
145 150 155 160
Ser Gln Glu His Asp Gly Ala Ala Pro Arg Ala Lys Ala Ala Pro Ala
165 170 175
Gln Ala Thr Thr Ser Pro Leu Ala Pro Ala Ala Ala Ile Ala Pro Pro
180 185 190
Pro Gln Ala Pro His Ser Ala Ala Pro Thr Ala Ser Ser Lys Ala Ala
195 200 205
Phe Phe Phe Val Ala Thr Ala Met Leu Gly Leu Tyr Ile Ile Leu
210 215 220
<210> 11
<211> 1837
<212> DNA
<213> 乌拉尔图小麦(Triticum urartu)
<400> 11
atggagagat cccgccgcct gctgctggtg gcgggcctgc tcgccgcgct gctcccggcg 60
gcggcggccg ccttcgggcc gcagccgggg gcgccgtgcg agcccacgct gctggcgacg 120
caggtggcgc tcttctgcgc gcccgacatg cccaccgcgc agtgctgcga gcccgtcgtc 180
gccgccgtcg acctcggcgg cggggtcccc tgcctctgcc gcgtcgccgc ggagccgcag 240
ctcgtcatgg cgggcctcaa cgccacccac ctcctcacgc tctacagctc ctgcggcggc 300
ctccgtcccg gcggcgccca cctcgccgcc gcctgcgaag gtacgcgcac gttcaccgcc 360
ctccgtccct ccctctctct gtctacgtgc agattttctg tgctctcttt cctgcttgcc 420
tagtacgtag tgttccatgg cctctcgggc cgctagcgct ccgatttgcg ttggtttcct 480
tgctgttctg ccggatctgt cggcacggcg cgcggcgtcg ggttctcgcc gtctcccgtg 540
gcgagcgacc tgcgcagcgc gcgcgcggcc tagctagctt cataccgctg taccttgaga 600
tacacggagc gatttagggt ctactctgag tatttcgtca tcgtatgatg catgtgccgc 660
tcgcgattgt ttcatcgatt tgagatctgt gcttgttccc gcgagttaag atggatctag 720
cgccgtacgc agatgcagag tctgttgctc gagttatctt atctactgtc gttcgactat 780
ggtatttgcc tgcttccttt tggctgggtt tatcgtgcag tggtagtaga catgtggacg 840
cgttcttctt attttgtgcc gaccatcgtc gagatacttt tcctgctaca gcgtttcatc 900
gcctgcacca tcccgttcgt gatagcactt ttgtgtcaaa ccgcaacgca gctttgcttt 960
ctgcggtatc ttctgccttg tttgtcgcct tgcttggtca aaactgagaa ctcttgctgt 1020
ttgatcgacc gaggacaatc gcctgccgtg cttttggctc tgcagtgcgt cgtctctgcc 1080
tcctttgcca aacgtttcca tgggggcact gcttttttgc atgcggtttc cgtagccttc 1140
ctctttcgtg aaaaaaggtt tgggtcaaat caaatggatc gcctattggc agagcagcag 1200
cagatagctg gctgtctcac agctttggca gaatcggtct gttgcctgcc accgtgtctc 1260
ttatcttgcc tgccactgtg tctcttttct tgttgcgcac gtcgtcacct cctcctactt 1320
tttttccagt tttgtttact tttgatgaaa tacggacgaa cggctggtaa ttattaactt 1380
tggttgctgt tgttactgtg gattttggac gcaggacccg ctcccccggc cgccgtcgtc 1440
agcagccccc cgcccccgcc accgtcgacc gcacctcgcc gcaagcagcc agcgcgtgcg 1500
tacctctccc tctcgcccgc atctcgctcc gtattagctg attgtgtctg catactgacg 1560
tgtgctttgg ctttggatct gtttcgcaga cgacgcacca ccgccgccgc cgccgtccag 1620
cgacaagccg tcgtccccgc cgccgtccca ggagcacgac ggcgccgctc cccacgcaaa 1680
ggccgccccc gcccaggcgg ctacctcccc gctcgcgccc gctgctgcca ttgccccgcc 1740
gccccaggcg ccacactccg ccgcgcccac ggcgtcatcc aaggcggcct tcttcttcgt 1800
cgccacggcc atgctcggcc tctacatcat cctctga 1837
<210> 12
<211> 669
<212> DNA
<213> 乌拉尔图小麦(Triticum urartu)
<400> 12
atggagagat cccgccgcct gctgctggtg gcgggcctgc tcgccgcgct gctcccggcg 60
gcggcggccg ccttcgggcc gcagccgggg gcgccgtgcg agcccacgct gctggcgacg 120
caggtggcgc tcttctgcgc gcccgacatg cccaccgcgc agtgctgcga gcccgtcgtc 180
gccgccgtcg acctcggcgg cggggtcccc tgcctctgcc gcgtcgccgc ggagccgcag 240
ctcgtcatgg cgggcctcaa cgccacccac ctcctcacgc tctacagctc ctgcggcggc 300
ctccgtcccg gcggcgccca cctcgccgcc gcctgcgaag gacccgctcc cccggccgcc 360
gtcgtcagca gccccccgcc cccgccaccg tcgaccgcac ctcgccgcaa gcagccagcg 420
cacgacgcac caccgccgcc gccgccgtcc agcgacaagc cgtcgtcccc gccgccgtcc 480
caggagcacg acggcgccgc tccccacgca aaggccgccc ccgcccaggc ggctacctcc 540
ccgctcgcgc ccgctgctgc cattgccccg ccgccccagg cgccacactc cgccgcgccc 600
acggcgtcat ccaaggcggc cttcttcttc gtcgccacgg ccatgctcgg cctctacatc 660
atcctctga 669
<210> 13
<211> 222
<212> PRT
<213> 乌拉尔图小麦(Triticum urartu)
<400> 13
Met Glu Arg Ser Arg Arg Leu Leu Leu Val Ala Gly Leu Leu Ala Ala
1 5 10 15
Leu Leu Pro Ala Ala Ala Ala Ala Phe Gly Pro Gln Pro Gly Ala Pro
20 25 30
Cys Glu Pro Thr Leu Leu Ala Thr Gln Val Ala Leu Phe Cys Ala Pro
35 40 45
Asp Met Pro Thr Ala Gln Cys Cys Glu Pro Val Val Ala Ala Val Asp
50 55 60
Leu Gly Gly Gly Val Pro Cys Leu Cys Arg Val Ala Ala Glu Pro Gln
65 70 75 80
Leu Val Met Ala Gly Leu Asn Ala Thr His Leu Leu Thr Leu Tyr Ser
85 90 95
Ser Cys Gly Gly Leu Arg Pro Gly Gly Ala His Leu Ala Ala Ala Cys
100 105 110
Glu Gly Pro Ala Pro Pro Ala Ala Val Val Ser Ser Pro Pro Pro Pro
115 120 125
Pro Pro Ser Thr Ala Pro Arg Arg Lys Gln Pro Ala His Asp Ala Pro
130 135 140
Pro Pro Pro Pro Pro Ser Ser Asp Lys Pro Ser Ser Pro Pro Pro Ser
145 150 155 160
Gln Glu His Asp Gly Ala Ala Pro His Ala Lys Ala Ala Pro Ala Gln
165 170 175
Ala Ala Thr Ser Pro Leu Ala Pro Ala Ala Ala Ile Ala Pro Pro Pro
180 185 190
Gln Ala Pro His Ser Ala Ala Pro Thr Ala Ser Ser Lys Ala Ala Phe
195 200 205
Phe Phe Val Ala Thr Ala Met Leu Gly Leu Tyr Ile Ile Leu
210 215 220
<210> 14
<211> 1885
<212> DNA
<213> 粗山羊草(Aegilops tauschii Coss)
<400> 14
atggagagat cccgcggcct gctgctggtg gcgggcctgc tggcggcgct gctgccggcg 60
gcggcggccg cgttcgggca gcagccgggg gcgccgtgcg agcccacgct gctggcgacg 120
caggtggcgc tcttctgcgc gcccgacatg cccacggccc agtgctgcga gcccgtcgtc 180
gccgccgtcg acctcggcgg cggggtgccc tgcctctgcc gcgtcgccgc ggagccgcag 240
ctcgtcatgg cgggcctcaa cgccacccat ctcctcacgc tctacggctc ctgcggcggc 300
ctccgtcctg gcggcgccca cctcgccgcc gcctgcgaag gtacgtcgcg cacgttcacc 360
gcctccctcc ctccctcgct ctctctctct ctctctctac gtgccgattc tctgtgttcg 420
cttccctgct tacctagcac gtagttttcc atggcttctc gactcgctgg tcctccgatt 480
tgggtcggtt aatttcctcg ctgtactacc ggatctgtcg gcacggcgcg cggcgtcggg 540
ttctcgccgt ctcccgtggc gagcgacctg cgcagcgcgc gcgcggccta gctagcttca 600
taccgctgta ccttcagata cacggagcga tttagggtct actctgagta tttcgtcatc 660
gtaggatgca tgtggcagtc gcgattgttt catcgatttt agatctgtgc ttgttcccgc 720
gagttaagat ggatctagcg ccgtacgcag acgcagatgg tcttgctgtc tctgttgctc 780
gagttatctt atctactgtc gttcgagtat atttgcctgc ttccttttga tctgtgttta 840
tcgtgcagta gcagtagcca tgtccacgcc ttcttgtttc gaggcgatca tcgtcgagat 900
agcgctttgt ttcaaaccgc aacgcagcct ttgctttctg cggtatcttc tgccttgttt 960
ttgttctgtg cagtacgtct tgcttggtca aaagtaaaaa ctcttgctgt tcgatcgacc 1020
gaggcctgat gcagagcaag agctggccgt gcttttcgct ctgcagtgca tcgcctctgc 1080
ctctttggcc aaacatttcc atgttgatcc tctggtgtgg tactactttt ttgcatgcgg 1140
tttgcgtagc cttcctcttt cgtgaaaaaa ggtcgggtcg cctattggca gagcagcagc 1200
agcagcaaca gatagctggc tgtctcgcag ctttgacaga accggtctgt ggccatctgt 1260
cgccgcctgc caccgtttcc ctgatgtttg tttctctcgt ctcatctcgc ctgccactgt 1320
ttcttttctt gttgcgcacg tcgtcacctc ctcctacttt tttttccagt tttgtttact 1380
tttgagatac ggacgaacgg ctggtaatta ctaactttgg ttgctgttgt tactgtggat 1440
tttggacgca ggacccgctc ccccggccgc catcgtcagc agccccccgc ccccgccacc 1500
accgtccgcc gcacctcgcc gcaagcagcc agcgcgtacg aacctctccc tccctctctc 1560
tcgcctgcat ctcgctctgt attagctgat tgtgtttact tactgacgtg tgctttggct 1620
ttggatctgt ttcgcagacg acgcaccgcc gccgccgccg ccgtctagcg agaagccgtc 1680
gtccccgccg ccgtcccagg agcacgacgg cgccgccccc cgcgccaagg ccgcgcccgc 1740
ccaggcgacc acctccccgc tcgcgcccgc tgccgccatc gccccgccgc cccaggcgcc 1800
acactccgcg gcgcccacgg cgtcgtccaa ggcggccttc ttcttcgtcg ccacggccat 1860
gctcggcctc tacatcatcc tctga 1885
<210> 15
<211> 672
<212> DNA
<213> 粗山羊草(Aegilops tauschii Coss)
<400> 15
atggagagat cccgcggcct gctgctggtg gcgggcctgc tggcggcgct gctgccggcg 60
gcggcggccg cgttcgggca gcagccgggg gcgccgtgcg agcccacgct gctggcgacg 120
caggtggcgc tcttctgcgc gcccgacatg cccacggccc agtgctgcga gcccgtcgtc 180
gccgccgtcg acctcggcgg cggggtgccc tgcctctgcc gcgtcgccgc ggagccgcag 240
ctcgtcatgg cgggcctcaa cgccacccat ctcctcacgc tctacggctc ctgcggcggc 300
ctccgtcctg gcggcgccca cctcgccgcc gcctgcgaag gacccgctcc cccggccgcc 360
atcgtcagca gccccccgcc cccgccacca ccgtccgccg cacctcgccg caagcagcca 420
gcgcacgacg caccgccgcc gccgccgccg tctagcgaga agccgtcgtc cccgccgccg 480
tcccaggagc acgacggcgc cgccccccgc gccaaggccg cgcccgccca ggcgaccacc 540
tccccgctcg cgcccgctgc cgccatcgcc ccgccgcccc aggcgccaca ctccgcggcg 600
cccacggcgt cgtccaaggc ggccttcttc ttcgtcgcca cggccatgct cggcctctac 660
atcatcctct ga 672
<210> 16
<211> 223
<212> PRT
<213> 粗山羊草(Aegilops tauschii Coss)
<400> 16
Met Glu Arg Ser Arg Gly Leu Leu Leu Val Ala Gly Leu Leu Ala Ala
1 5 10 15
Leu Leu Pro Ala Ala Ala Ala Ala Phe Gly Gln Gln Pro Gly Ala Pro
20 25 30
Cys Glu Pro Thr Leu Leu Ala Thr Gln Val Ala Leu Phe Cys Ala Pro
35 40 45
Asp Met Pro Thr Ala Gln Cys Cys Glu Pro Val Val Ala Ala Val Asp
50 55 60
Leu Gly Gly Gly Val Pro Cys Leu Cys Arg Val Ala Ala Glu Pro Gln
65 70 75 80
Leu Val Met Ala Gly Leu Asn Ala Thr His Leu Leu Thr Leu Tyr Gly
85 90 95
Ser Cys Gly Gly Leu Arg Pro Gly Gly Ala His Leu Ala Ala Ala Cys
100 105 110
Glu Gly Pro Ala Pro Pro Ala Ala Ile Val Ser Ser Pro Pro Pro Pro
115 120 125
Pro Pro Pro Ser Ala Ala Pro Arg Arg Lys Gln Pro Ala His Asp Ala
130 135 140
Pro Pro Pro Pro Pro Pro Ser Ser Glu Lys Pro Ser Ser Pro Pro Pro
145 150 155 160
Ser Gln Glu His Asp Gly Ala Ala Pro Arg Ala Lys Ala Ala Pro Ala
165 170 175
Gln Ala Thr Thr Ser Pro Leu Ala Pro Ala Ala Ala Ile Ala Pro Pro
180 185 190
Pro Gln Ala Pro His Ser Ala Ala Pro Thr Ala Ser Ser Lys Ala Ala
195 200 205
Phe Phe Phe Val Ala Thr Ala Met Leu Gly Leu Tyr Ile Ile Leu
210 215 220
<210> 17
<211> 1750
<212> DNA
<213> 高粱(Sorghum bicolor)
<400> 17
atggagcgcc gcctccacca cctcgcgctg ctgctcggcc tcctcgcctg ggcggcggcg 60
gccgccacca cgggagccga ggcgcagccg gcggtgtgcg acccctccat catcgccacc 120
cagatcgcgc tcttctgcat gccggacatg cccacggcgc cctgctgcga gcccatcatc 180
gcctccgtcg acctcggcgg cggcatcccc tgcctctgcc gcgtcgccgc ccaaccgcag 240
ctcgtcctcg cccgcctcaa cgccacccac ctcctcgcgc tctacgcctc ctgcggagga 300
cagcacaccg ggggcgccca cctcgccgcc gcctgccaag gtacatcacg cgttcgcctc 360
tctctctctc tctctctctc tctctctctc tctctctctc tctctctctc tctctctcgt 420
gcgagtgtga gtgtgtatgt ttggctgtgt gcgtgaggtg agggtgtgtg tttgctcgga 480
tccagtggtg gtttggcgag atcctagtgt actgtctgtg cttgccgatt tggttcttga 540
ctcttgagcc ttttgcgtgc gtttatgatg gtatatatat ttcggtgcga gttctcgcct 600
tccactgtgg cgacgaaccg tacgtactga tcaagtttgt tcgaacttaa ttttgcttca 660
tgtcgtcgtc tctttcgagt cgcaagaccg ctactactag tgggcatatt ttgggtgaga 720
ttacccgttg ctccgtacgc gcgcggctcc tggtctttgt agaccttttc tctttggttt 780
ggatttcgtt cgttttgttc tggatttcga aaaaaagttt gttcgtcttg gttccccact 840
ctgggcttaa ttttccccat ggatcacctg atgttttgac ccgtttctga tgtttttttt 900
ttgctaaaaa gtatttgtgc ttatcaagca ggtctgcgtt tctttttcgc ctcttccttt 960
ttgcgttctt gtctgcttgt tcctgttgcg tgaaaagttt cctggcctga attttgaaca 1020
gagagagagc aactgccctc tgggcttctg gcttttgcag cagcaggcca gctgcacaac 1080
ctgcagcagc gctgcagcct ttcaggattt cggcaaagac catcccattc ttttgcccgt 1140
ttgttttctt ggcccgcacg aaatagcgcc acgtcgcatg cttttctacc cgaaagacga 1200
tactgccctt gcactaatta tctttccgtt gttttccttt tgtggacgtg aaattattgc 1260
atctctggac taacgccgtt aaactctcgt gctgtttgcc tgcaggtccg tcacctcccg 1320
ccgccaccat tcccgtgatc gccccgccgc cgcccgccgc gccccgccac aagcagccaa 1380
cacgtacgaa cccgctgtca cctcctcgat ctgctctgtg ctatttctgc tttaactttt 1440
cgcgtgttct ctgtttgatc cgtgcgggac gggacggtgt cttatgtttc tgttgcgatg 1500
atgtttcgca ggcgaggccc ctcccccgcc gctgcggggc gagaagccgt ccccgtcgcc 1560
ccagcagcag cctggcgccg ccgccgccca cggcaaggcc atccccgcca gcccggccgc 1620
ctccttctcc cagctggcgc cggccgccgc gcccacgacg cccacgccac cgcactccgg 1680
ctccgacccc atcgtcgcct ccgccgcgct cctcgtcttc ttcatcgccg tcctcatcat 1740
cctcgactga 1750
<210> 18
<211> 657
<212> DNA
<213> 高粱(Sorghum bicolor)
<400> 18
atggagcgcc gcctccacca cctcgcgctg ctgctcggcc tcctcgcctg ggcggcggcg 60
gccgccacca cgggagccga ggcgcagccg gcggtgtgcg acccctccat catcgccacc 120
cagatcgcgc tcttctgcat gccggacatg cccacggcgc cctgctgcga gcccatcatc 180
gcctccgtcg acctcggcgg cggcatcccc tgcctctgcc gcgtcgccgc ccaaccgcag 240
ctcgtcctcg cccgcctcaa cgccacccac ctcctcgcgc tctacgcctc ctgcggagga 300
cagcacaccg ggggcgccca cctcgccgcc gcctgccaag gtccgtcacc tcccgccgcc 360
accattcccg tgatcgcccc gccgccgccc gccgcgcccc gccacaagca gccaacacgc 420
gaggcccctc ccccgccgct gcggggcgag aagccgtccc cgtcgcccca gcagcagcct 480
ggcgccgccg ccgcccacgg caaggccatc cccgccagcc cggccgcctc cttctcccag 540
ctggcgccgg ccgccgcgcc cacgacgccc acgccaccgc actccggctc cgaccccatc 600
gtcgcctccg ccgcgctcct cgtcttcttc atcgccgtcc tcatcatcct cgactga 657
<210> 19
<211> 218
<212> PRT
<213> 高粱(Sorghum bicolor)
<400> 19
Met Glu Arg Arg Leu His His Leu Ala Leu Leu Leu Gly Leu Leu Ala
1 5 10 15
Trp Ala Ala Ala Ala Ala Thr Thr Gly Ala Glu Ala Gln Pro Ala Val
20 25 30
Cys Asp Pro Ser Ile Ile Ala Thr Gln Ile Ala Leu Phe Cys Met Pro
35 40 45
Asp Met Pro Thr Ala Pro Cys Cys Glu Pro Ile Ile Ala Ser Val Asp
50 55 60
Leu Gly Gly Gly Ile Pro Cys Leu Cys Arg Val Ala Ala Gln Pro Gln
65 70 75 80
Leu Val Leu Ala Arg Leu Asn Ala Thr His Leu Leu Ala Leu Tyr Ala
85 90 95
Ser Cys Gly Gly Gln His Thr Gly Gly Ala His Leu Ala Ala Ala Cys
100 105 110
Gln Gly Pro Ser Pro Pro Ala Ala Thr Ile Pro Val Ile Ala Pro Pro
115 120 125
Pro Pro Ala Ala Pro Arg His Lys Gln Pro Thr Arg Glu Ala Pro Pro
130 135 140
Pro Pro Leu Arg Gly Glu Lys Pro Ser Pro Ser Pro Gln Gln Gln Pro
145 150 155 160
Gly Ala Ala Ala Ala His Gly Lys Ala Ile Pro Ala Ser Pro Ala Ala
165 170 175
Ser Phe Ser Gln Leu Ala Pro Ala Ala Ala Pro Thr Thr Pro Thr Pro
180 185 190
Pro His Ser Gly Ser Asp Pro Ile Val Ala Ser Ala Ala Leu Leu Val
195 200 205
Phe Phe Ile Ala Val Leu Ile Ile Leu Asp
210 215
<210> 20
<211> 1690
<212> DNA
<213> 玉米(Zea mays L.)
<400> 20
atggagctcc gcctctacca cctcgcgctg ctgctcggcc tcgtcgcctg ggcggcggct 60
gctgccacgg gagccgaggc gcagccggtg tgcgacccct ccatcatcgc cacccagatc 120
gcgctcttct gcatgccgga catgcccacg gcgccctgct gcgagccgat catcgcctcc 180
gtcgacctcg gcggcggcgt cccctgcctc tgccgcgtcg ccgcccaacc gcagctcgtc 240
ctcgcccgcc tcaacgccac ccacctcctc gcgctctacg cttcctgcgg aggacaacac 300
accgtgggcg ctcacctcgc tgccgcctgc caaggtacat cacggtcgca ctctctctct 360
ctctctctct ctctctgtgc gtgcgtgacc tgtgcgcgtg agtgcggtgt gtgcgcgagg 420
gtgtgtgttc gctcggatcg agtggtggtc tgccgtgatc ctagtgtact gttctactgt 480
gtcttcggat ttggtttttg agcttttggc gtgcgttttt gtgtcatggt atttcgttgc 540
gagttcccgt cttccgctgt ggcgacgaac ccgtaccgta cgtcttgata aagtttgttc 600
gatttttctt cgcaagagcg ctactactgg gtgggcatat ttggggcgag atcaccagtg 660
gctccatagt ccatacgcgc gcgactcgtg ttttttagcc tattctattt ggcctgaatt 720
ttgaaaaagt ttgttcgttt aattggttgc cttctctggg cttgttttgc cccattgatc 780
acgtgacccg tttctgatgt ttttgcttac tgaaatatac atttgcttta gtattaagcg 840
acgcacggac gctgcgtttc tttttcactt attccttttt gcgtcattgt ctgcttgttc 900
tttgttgctc gaaagtttgc tggcctgaat tctgaacata gagagtaact gccctctggg 960
ctctggcctc tgcaggagca ggctgcaagc cggttgcaga acctgcagca gcgctgcagc 1020
ctttcaggat gccggcaaag accagccccg tttctcttgc ccgtttgttt tcttggcccg 1080
cacgaaatgg cgccgcgtcg catgcttttc tacgcgagag acgatactgc ccttgcacta 1140
attatcactc ttgttgtttt atttttgaac gtgaaattat tgcatgtctg ggcggactat 1200
aacgctgtta aaaacttaaa atcacgtgct attgcctgca ggtccgtcac ctcccgccac 1260
cgttcctgtt atcgccccgc cgccgcccgc cgcgccccgc ctcaagcagc caacacgtac 1320
gaagccgctg tcacctagtc tgctctgtgc tatttccgct ttaacttttc gcgcgctctg 1380
tttgattgat gcgagaattg gactaatccg tgctgtgcat aattttctgt tgcgatgttt 1440
ggcaggcgag gcccctcccc ctccgccaca aagcgagaag ccgtccccgt cgccccagca 1500
gcagcctggc gtcgccgccg cccacggcaa ggccatcccc accagttcgg ccgccaactt 1560
ctccacgctg gcgccggccg ccgcgcccac gactcccacg ccaccacact ctggctccga 1620
caacgggtgc gtcgtcgtct cctccacgat tgtcatcttc gtcatcgccg ttatcaccat 1680
cctcgaatga 1690
<210> 21
<211> 654
<212> DNA
<213> 玉米(Zea mays L.)
<400> 21
atggagctcc gcctctacca cctcgcgctg ctgctcggcc tcgtcgcctg ggcggcggct 60
gctgccacgg gagccgaggc gcagccggtg tgcgacccct ccatcatcgc cacccagatc 120
gcgctcttct gcatgccgga catgcccacg gcgccctgct gcgagccgat catcgcctcc 180
gtcgacctcg gcggcggcgt cccctgcctc tgccgcgtcg ccgcccaacc gcagctcgtc 240
ctcgcccgcc tcaacgccac ccacctcctc gcgctctacg cttcctgcgg aggacaacac 300
accgtgggcg ctcacctcgc tgccgcctgc caaggtccgt cacctcccgc caccgttcct 360
gttatcgccc cgccgccgcc cgccgcgccc cgcctcaagc agccaacacg cgaggcccct 420
ccccctccgc cacaaagcga gaagccgtcc ccgtcgcccc agcagcagcc tggcgtcgcc 480
gccgcccacg gcaaggccat ccccaccagt tcggccgcca acttctccac gctggcgccg 540
gccgccgcgc ccacgactcc cacgccacca cactctggct ccgacaacgg gtgcgtcgtc 600
gtctcctcca cgattgtcat cttcgtcatc gccgttatca ccatcctcga atga 654
<210> 22
<211> 217
<212> PRT
<213> 玉米(Zea mays L.)
<400> 22
Met Glu Leu Arg Leu Tyr His Leu Ala Leu Leu Leu Gly Leu Val Ala
1 5 10 15
Trp Ala Ala Ala Ala Ala Thr Gly Ala Glu Ala Gln Pro Val Cys Asp
20 25 30
Pro Ser Ile Ile Ala Thr Gln Ile Ala Leu Phe Cys Met Pro Asp Met
35 40 45
Pro Thr Ala Pro Cys Cys Glu Pro Ile Ile Ala Ser Val Asp Leu Gly
50 55 60
Gly Gly Val Pro Cys Leu Cys Arg Val Ala Ala Gln Pro Gln Leu Val
65 70 75 80
Leu Ala Arg Leu Asn Ala Thr His Leu Leu Ala Leu Tyr Ala Ser Cys
85 90 95
Gly Gly Gln His Thr Val Gly Ala His Leu Ala Ala Ala Cys Gln Gly
100 105 110
Pro Ser Pro Pro Ala Thr Val Pro Val Ile Ala Pro Pro Pro Pro Ala
115 120 125
Ala Pro Arg Leu Lys Gln Pro Thr Arg Glu Ala Pro Pro Pro Pro Pro
130 135 140
Gln Ser Glu Lys Pro Ser Pro Ser Pro Gln Gln Gln Pro Gly Val Ala
145 150 155 160
Ala Ala His Gly Lys Ala Ile Pro Thr Ser Ser Ala Ala Asn Phe Ser
165 170 175
Thr Leu Ala Pro Ala Ala Ala Pro Thr Thr Pro Thr Pro Pro His Ser
180 185 190
Gly Ser Asp Asn Gly Cys Val Val Val Ser Ser Thr Ile Val Ile Phe
195 200 205
Val Ile Ala Val Ile Thr Ile Leu Glu
210 215
<210> 23
<211> 1600
<212> DNA
<213> 玉米(Zea mays L.)
<400> 23
atggaatgcc gcctccacca cctcgcgctg ctgctcggcc tcctcgtctg ggcggcggcg 60
gccgccacgg gaggcggggc gcagccggtg tgcgagccct ccatcatcgc cacccagatc 120
gcgctcttct gcatgccgga catgcccaca gcgccctgct gtgagccgat catcgcttcc 180
atcgacctcg gcggcggcgt cccctgccta tgccgcgtcg ccgcccagcc gcagctcgtc 240
ctcgcccgcc tcaacgccac ccaccacctc gcgctctacg cctcctgcgg aggacaacac 300
actatgggcg cccacctcgc cgccgcctgc caacaaggta catcatgttc gtctctcttt 360
atctctctct gtgtgtgaga gagagtgtgt gtgtggcctg tgcgcgtgag tgcgcaagtg 420
tgtgtacgct cggatccagt ggtggtttgg ctagtgtact gtactactgt gtctgtggct 480
atgtgccttc agatttggtt cttagctttt tgcgttcgtc tttgcttgat ggtgtttcgt 540
tgcgagtttc cgtacgtatt ccgctgtggc gacgaactcg tacgtcttga tcaaagtttg 600
ttcgattttt ttgctacatt attcgtctct ttcgggtcgc aagagcggta ctactagtgg 660
gcatgtttcg ggtgagattg cccgagcgac cgagtctccg tacgcgcgcg actgctgtgg 720
tttttgaggt cttcctcttt gtctgtggtt ccccttcccc gcaccccacc ccatgaatca 780
ggtatgattt aacccgtttc tgatgttttt tttgccctta agcagagctc cgaacgcagg 840
aacgctgttt tttttttttt gccctttagg gtttcctggc ctgaactctc aacagagaga 900
gggagagcaa ctgccctctg ccctttgcag cagcaggctg gccagctgca caacctgcac 960
tcctgcaggc agcagcgctt gcagcctctc agaggattcc ggcaaagacc caccccgttt 1020
ctcttgctcc atttgttttc ttggcccgca cgaaatggcg ccgcgggcgc atgctgctgt 1080
atacgacact gcccttgcac tagttatctt ttgtgttttg tgtacaggaa acgtttctgc 1140
tgttctcctt ttgagcccga aaactattgc atgtctggag agtggagact aacgccgtta 1200
aactctcgtt ctttctttgc ccccagctcc gtcacctccc gccaccgtcc tcgtgctcgc 1260
cccgccgccg ccacaagcag ccaacacgta cgtacgaacc cgctgtcacc tactctgctc 1320
tgtgctattt cttctgcttt aacttatttt agtgttccgt ttgattcatg cgagagttgg 1380
gctactaatc cgtgccgcgc ggcaatgttt ctgccgtgcc gtgtgctgtt tcgcaggcga 1440
gacgacccct cccccgctgc agccgagcaa gaagccgtcc cctacagggg ccaccgccgc 1500
cgcccacggc ctggccaacc ccacgccagc ccggccgccg cgcccacgat gcccccgccc 1560
ccgccaccgc actccggctc cgacaagggc tgcgtcgtag 1600
<210> 24
<211> 576
<212> DNA
<213> 玉米(Zea mays L.)
<400> 24
atggaatgcc gcctccacca cctcgcgctg ctgctcggcc tcctcgtctg ggcggcggcg 60
gccgccacgg gaggcggggc gcagccggtg tgcgagccct ccatcatcgc cacccagatc 120
gcgctcttct gcatgccgga catgcccaca gcgccctgct gtgagccgat catcgcttcc 180
atcgacctcg gcggcggcgt cccctgccta tgccgcgtcg ccgcccagcc gcagctcgtc 240
ctcgcccgcc tcaacgccac ccaccacctc gcgctctacg cctcctgcgg aggacaacac 300
actatgggcg cccacctcgc cgccgcctgc caacaagctc cgtcacctcc cgccaccgtc 360
ctcgtgctcg ccccgccgcc gccacaagca gccaacacgt acgtacgaac ccgcgagacg 420
acccctcccc cgctgcagcc gagcaagaag ccgtccccta caggggccac cgccgccgcc 480
cacggcctgg ccaaccccac gccagcccgg ccgccgcgcc cacgatgccc ccgcccccgc 540
caccgcactc cggctccgac aagggctgcg tcgtag 576
<210> 25
<211> 191
<212> PRT
<213> 玉米(Zea mays L.)
<400> 25
Met Glu Cys Arg Leu His His Leu Ala Leu Leu Leu Gly Leu Leu Val
1 5 10 15
Trp Ala Ala Ala Ala Ala Thr Gly Gly Gly Ala Gln Pro Val Cys Glu
20 25 30
Pro Ser Ile Ile Ala Thr Gln Ile Ala Leu Phe Cys Met Pro Asp Met
35 40 45
Pro Thr Ala Pro Cys Cys Glu Pro Ile Ile Ala Ser Ile Asp Leu Gly
50 55 60
Gly Gly Val Pro Cys Leu Cys Arg Val Ala Ala Gln Pro Gln Leu Val
65 70 75 80
Leu Ala Arg Leu Asn Ala Thr His His Leu Ala Leu Tyr Ala Ser Cys
85 90 95
Gly Gly Gln His Thr Met Gly Ala His Leu Ala Ala Ala Cys Gln Gln
100 105 110
Ala Pro Ser Pro Pro Ala Thr Val Leu Val Leu Ala Pro Pro Pro Pro
115 120 125
Gln Ala Ala Asn Thr Tyr Val Arg Thr Arg Glu Thr Thr Pro Pro Pro
130 135 140
Leu Gln Pro Ser Lys Lys Pro Ser Pro Thr Gly Ala Thr Ala Ala Ala
145 150 155 160
His Gly Leu Ala Asn Pro Thr Pro Ala Arg Pro Pro Arg Pro Arg Cys
165 170 175
Pro Arg Pro Arg His Arg Thr Pro Ala Pro Thr Arg Ala Ala Ser
180 185 190
<210> 26
<211> 1856
<212> DNA
<213> 粟(Setaria italica)
<400> 26
atggagcgcc ccctccgcca cctcgcgctg ctgctcggcc tcctcgcctg ggcggcggcg 60
gccgccaccg gggccgcggc gcagcctgcg tgcgagccct ccaacctcgc cacgcagatc 120
acgctcttct gcatgcccga catgcccacg gcgccctgct gcgagccggt cgtcgcctcc 180
gtagacctcg gcggcggcgt cccctgcctc tgccgcgtcg ccgcccagcc gcagctcgtc 240
ctcgcccgcc tcaacgcctc ccacctcctc gcgctctaca ccgcctgcgg gggactgcgc 300
accgggggcg cccacctcgc cgccgcctgc caaggtacgt gacaccagcg cccgcgcctc 360
tcctctctct agatctgcgt ctgtgccgtg gccgtgcgcg agatgggtgt gctaggtgcg 420
agatgtgtgt gttctgcagc tttctggttt ggctcttgag cgagctgttc gtggttgcgc 480
tcttgggtaa cggtgttttg ttgcgagttc ccgtcttcct ctgtggcgac gaacccgtac 540
gtcttgttcc agtacgttcg aacatttttt tttgcttcgt tttcttctct cctccgtatt 600
ttggctatgg ttctcgcgtc acatgaatgc tactagttgg gcacatttcg ggatagattt 660
acccgtacgt cctgttctgg tttgttcgaa cacttttcct tcgttttctt ttctcttccg 720
aattttggtt gtgttttcgg gacacgcgag cgctactact agttgggcaa atttggggtc 780
agatgtaccc gtggctccgt acgcgcgcac gtctcctgat ttatttgtgc gtttcctttt 840
ctcaagcgat ttctctgtgc cttttcgttt tggtttggaa gcaaattctc tgggtttcaa 900
ggcgttggtt ccgatctctg ggcttttagc catgcatctc ctgatgattt gacccgtttc 960
agtggagtgg aatctgttca actgcccaag cgaggtcgaa aggctgggtt tctttgccca 1020
agcgaggtcg aaaggctggg tttctttttg ctttttcacc gcttcctttt tttgtttttg 1080
tctccttgtt tccgttgcgt cgaaaggctt gctgcgttcc agccttctga acagagagag 1140
caaccgccct ctgatcctct gcagcggaca gctgcacagc ctgcacagca gcgctgcagc 1200
ctttcaggca agaccaagcc cgttcggcac cgtttctctc tcccgcttgg tttcttggcc 1260
ctcacgaaat ggcgcctcgg cgcatgcttt ttctgcccaa aagacgatac tacccctgca 1320
ataaatatct tctgttattt ttgccgtttt aatctgtgaa ttattacaag cctggactaa 1380
cggcgttaac ctctcgtgtt tcgtgcctgc aggtccgtca ccccctgcca ccgtccccgt 1440
catcgccccg ccgccgcccg ccgcccctcg ccacaggctg ccagcacgta cgaacccgcc 1500
cgcacccgct ctgctcctgc tactttccgc tttaactttt cgcgtgctct gtttgatcga 1560
tgcgagagtt gaactaatgc atgtgctggt ttctgtttct cttgtgctgt tgcgcaggcg 1620
gcgaggcccc tccccctccg gcgacgagcg agaagccgtc cccaccaccc cagcagcagc 1680
ctggcgccgc cgccgcccac ggcaaggcca tccccgccag cccggccgcc tcctcgccgc 1740
tggcgccggc agccgcgccc acgacgccca cgccgccaac ctccggctcc gacggcaggt 1800
gcagctgcac cccggtcatc ctcttcctcc tcaccgccat catcgtcctc tactga 1856
<210> 27
<211> 648
<212> DNA
<213> 粟(Setaria italica)
<400> 27
atggagcgcc ccctccgcca cctcgcgctg ctgctcggcc tcctcgcctg ggcggcggcg 60
gccgccaccg gggccgcggc gcagcctgcg tgcgagccct ccaacctcgc cacgcagatc 120
acgctcttct gcatgcccga catgcccacg gcgccctgct gcgagccggt cgtcgcctcc 180
gtagacctcg gcggcggcgt cccctgcctc tgccgcgtcg ccgcccagcc gcagctcgtc 240
ctcgcccgcc tcaacgcctc ccacctcctc gcgctctaca ccgcctgcgg gggactgcgc 300
accgggggcg cccacctcgc cgccgcctgc caaggtccgt caccccctgc caccgtcccc 360
gtcatcgccc cgccgccgcc cgccgcccct cgccacaggc tgccagcacg cggcgaggcc 420
cctccccctc cggcgacgag cgagaagccg tccccaccac cccagcagca gcctggcgcc 480
gccgccgccc acggcaaggc catccccgcc agcccggccg cctcctcgcc gctggcgccg 540
gcagccgcgc ccacgacgcc cacgccgcca acctccggct ccgacggcag gtgcagctgc 600
accccggtca tcctcttcct cctcaccgcc atcatcgtcc tctactga 648
<210> 28
<211> 215
<212> PRT
<213> 粟(Setaria italica)
<400> 28
Met Glu Arg Pro Leu Arg His Leu Ala Leu Leu Leu Gly Leu Leu Ala
1 5 10 15
Trp Ala Ala Ala Ala Ala Thr Gly Ala Ala Ala Gln Pro Ala Cys Glu
20 25 30
Pro Ser Asn Leu Ala Thr Gln Ile Thr Leu Phe Cys Met Pro Asp Met
35 40 45
Pro Thr Ala Pro Cys Cys Glu Pro Val Val Ala Ser Val Asp Leu Gly
50 55 60
Gly Gly Val Pro Cys Leu Cys Arg Val Ala Ala Gln Pro Gln Leu Val
65 70 75 80
Leu Ala Arg Leu Asn Ala Ser His Leu Leu Ala Leu Tyr Thr Ala Cys
85 90 95
Gly Gly Leu Arg Thr Gly Gly Ala His Leu Ala Ala Ala Cys Gln Gly
100 105 110
Pro Ser Pro Pro Ala Thr Val Pro Val Ile Ala Pro Pro Pro Pro Ala
115 120 125
Ala Pro Arg His Arg Leu Pro Ala Arg Gly Glu Ala Pro Pro Pro Pro
130 135 140
Ala Thr Ser Glu Lys Pro Ser Pro Pro Pro Gln Gln Gln Pro Gly Ala
145 150 155 160
Ala Ala Ala His Gly Lys Ala Ile Pro Ala Ser Pro Ala Ala Ser Ser
165 170 175
Pro Leu Ala Pro Ala Ala Ala Pro Thr Thr Pro Thr Pro Pro Thr Ser
180 185 190
Gly Ser Asp Gly Arg Cys Ser Cys Thr Pro Val Ile Leu Phe Leu Leu
195 200 205
Thr Ala Ile Ile Val Leu Tyr
210 215
<210> 29
<211> 1487
<212> DNA
<213> 人工合成
<400> 29
atggcggcga caatggcagt gacgacgatg gtgacgagga gcaaggagag ctggtcgtca 60
ttgcaggtcc cggcggtggc attcccttgg aagccacgag gtggcaagac cggcggcctc 120
gagttccctc gccgggcgat gttcgccagc gtcggcctca acgtgtgccc gggcgtcccg 180
gcggggcgcg acccgcggga gcccgatccc aaggtcgtcc gggcggcctg cggcctggtc 240
caggcacaag tcctcttcca ggggtttaac tgggagtcgt gcaagcagca gggaggctgg 300
tacaacaggc tcaaggccca ggtcgacgac atcgccaagg ccggcgtcac gcacgtctgg 360
ctgcctccac cctcgcactc cgtctcgcca caaggctaca tgccaggccg cctatacgac 420
ctggacgcgt ccaagtacgg cacggcggcg gagctcaagt ccctgatagc ggcgttccac 480
ggcaggggcg tgcagtgcgt ggcggacatc gtcatcaacc accggtgcgc ggaaaagaag 540
gacgcgcgcg gcgtgtactg catcttcgag ggcgggactc ccgacgaccg cctggactgg 600
ggccccggga tgatctgcag cgacgacacg cagtactcgg acgggacggg gcaccgcgac 660
cgggcgaggg gttcgcggcg gcgcccgaca tcgaccacct caacccgcgc gtgcagcggg 720
agctctccgc ctggctcaac tggctcaggt ccgacgccgt ggggttcgac ggctggcgcc 780
tcgacttcgc caagggctac tcgccggccg tcgccagaat gtacgtggag agcacggggc 840
cgccgagctt cgtcgtcgcg gagatatgga actcgctgag ctacagcggg gacggcaagc 900
cggcgcccaa ccaggaccag tgccggcagg agctgctgga ctggacgcgg gccgtcggcg 960
ggcccgccat ggcgttcgac ttccccacca agggcctgct gcaggcgggc gtgcaggggg 1020
agctgtggcg gctgcgcgac agctccggca acgcggccgg cctgatcggg tgggcgcccg 1080
agaaggccgt caccttcgtc gacaaccatg acaccgggtc gacgcagaag ctctggccgt 1140
tcccatccga caaggtcatg cagggctacg cctacatcct cacccatcca ggagtcccct 1200
gcattttcta cgaccacatg ttcgactgga acctgaagca ggagatatcc acgctgtctg 1260
ccatcagggc gcggaacggc atccgcgccg ggagcaagct gcggatcctc gtggcggacg 1320
cggacgcgta cgtggccgtc gtcgacgaga aggtcatggt gaagatcggg acaaggtacg 1380
gcgtgagcag cgtggtcccg tcggatttcc acccggcggc gcacggcaag gactactgcg 1440
tctgggagaa agcgagcctc cgcgtcccgg cggggcgcca cctctag 1487
<210> 30
<211> 2736
<212> DNA
<213> 玉米(Zea mays)
<400> 30
tgcaccggac actgtctggt ggcataccag acagtccggt gtgccagatc agggcaccct 60
tcggttcctt tgctcctttg cttttgaacc ctaactttga tcgtttattg gtttgtgttg 120
aacctttatg cacctgtgga atatataatc tagaacaaac tagttagtcc aatcatttgt 180
gttgggcatt caaccaccaa aattatttat aggaaaaggt taaaccttat ttccctttca 240
atctccccct ttttggtgat tgatgccaac acaaaccaaa gaaaatatat aagtgcagaa 300
ttgaactagt ttgcataagg taagtgcata ggttacttag aattaaatca atttatactt 360
ttacttgata tgcatggttg ctttctttta ttttaacatt ttggaccaca tttgcaccac 420
ttgttttgtt ttttgcaaat ctttttggaa attctttttc aaagtctttt gcaaatagtc 480
aaaggtatat gaataagatt gtaagaagca ttttcaagat ttgaaatttc tccccctgtt 540
tcaaatgctt ttcctttgac taaacaaaac tccccctgaa taaaattctc ctcttagctt 600
tcaagagggt tttaaataga tatcaattgg aaatatattt agatgctaat tttgaaaata 660
taccaattga aaatcaacat accaatttga aattaaacat accaatttaa aaaatttcaa 720
aaagtggtgg tgcggtcctt ttgctttggg cttaatattt ctcccccttt ggcattaatc 780
gccaaaaacg gagactttgt gagccattta tactttctcc ccattggtaa atgaaatatg 840
agtgaaagat tataccaaat ttggacagtg atgcggagtg acggcgaagg ataaacgata 900
ccgttagagt ggagtggaag ccttgtcttc gccgaagact ccatttccct ttcaatctac 960
gacttagcat agaaatacac ttgaaaacac attagtcgta gccacgaaag agatatgatc 1020
aaaggtatac aaatgagcta tgtgtgtaat gtttcaatca aagtttcgag aatcaagaat 1080
atttagctca ttcctaagtt tgctaaaggt tttatcatct aatggtttgg taaagatatc 1140
gactaattgt tctttggtgc taacataagc aatctcgata tcaccccttt gttggtgatc 1200
cctcaaaaag tgataccgaa tgtctatgtg cttagtgcgg ctgtgttcaa cgggattatc 1260
cgccatgcag atagcactct cattgtcaca taggagaggg actttgctca atttgtagcc 1320
atagtcccta aggttttgcc tcatccaaag taattgcaca caacaatgtc ctgcggcaat 1380
atacttggct tcggcggtag aaagagctat tgagttttgt ttctttgaag tccaagacac 1440
cagggatctc cctagaaact gacaagtccc tgatgtgctc ttcctatcaa ttttacaccc 1500
tgcccaatcg gcatctgaat atcctattaa atcaaaggtg gatcccttgg ggtaccaaag 1560
accaaattta ggagtgtaaa ctaaatatct catgattctt ttcacggccc taaggtgaac 1620
ttccttagga tcggcttgga atcttgcaca catgcatata gaaagcatac tatctggtcg 1680
agatgcacat aaatagagta aagatcctat catcgaccgg tatacctttt ggtctacgga 1740
tttacctccc gtgtcgaggt cgagatgccc attagttccc atgggtgtcc tgatgggctt 1800
ggcatccttc attccaaact tgttgagtat gtcttgaatg tactttgttt ggctgatgaa 1860
ggtgccatct tggagttgct tgacttgaaa tcctagaaaa tatttcaact tccccatcat 1920
agacatctcg aatttcggaa tcatgatcct actaaactct tcacaagtag atttgttagt 1980
agacccaaat ataatatcat caacataaat ttggcataca aacaaaactt ttgaaatggt 2040
tttagtaaag agagtaggat cggctttact gactctgaag ccattagtga taagaaaatc 2100
tcttaggcat tcataccatg ctgttggggc ttgcttgagc ccataaagcg cctttgagag 2160
tttataaaca tggttagggt actcactatc ttcaaagccg agaggttgct caacatagac 2220
ctattcaccc catttgatca cttttttggt ccttcaggat ctaatagtta tgtataattt 2280
agagtctctt gtttaatggc cagatatttc taattaatct aagaatttat gatatttttt 2340
aattttttat catgtctgat gagaattaac ataaaggctc aattgggtcc tgaattaata 2400
atagagtgaa aattaatcca gaggctctat tagaaccttc aattagtaat accaagatat 2460
atataagata gtagagtata gtttaaatgt tggcattgtt cattctttct tttgttattt 2520
aatttatgct ttccacggtg gttagtggtt acttctgaag ggtccaaata atgcatgaag 2580
agtttgagga caagaagtct gccctaaaaa tagcgatgca aaggcatggt gtccaagcca 2640
tacatatagc gcactaattt tatcagcaga acaatggtat ttataggtcc tagtgcccag 2700
gcaacaagag acacgaataa agcatcgatc acgaca 2736
<210> 31
<211> 344
<212> DNA
<213> 玉米(Zea mays)
<400> 31
tgacaaagca gcattagtcc gttgatcggt ggaagaccac tcgtcagtgt tgagttgaat 60
gtttgatcaa taaaatacgg caatgctgta agggttgttt tttatgccat tgataataca 120
ctgtactgtt cagttgttga actctatttc ttagccatgc caagtgcttt tcttattttg 180
aataacatta cagcaaaaag ttgaaagaca aaaaaaaaaa cccccgaaca gagtgctttg 240
ggtcccaagc tactttagac tgtgttcggc gttcccccta aatttctccc cctatatctc 300
actcacttgt cacatcagcg ttctctttcc cctatatctc cacg 344
<210> 32
<211> 711
<212> DNA
<213> 人工合成
<400> 32
atggtgtcca agggcgagga ggacaacatg gccatcatca aggagttcat gcgcttcaag 60
gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc 120
cgcccctacg agggcaccca aaccgccaag ctcaaggtga ccaagggtgg ccccctcccc 180
ttcgcctggg acatcctctc cccacaattc atgtacggct ccaaggccta cgtgaagcac 240
cccgccgaca tccccgacta cctcaagctc tccttccccg agggcttcaa gtgggagcgc 300
gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aagactcctc cctccaagac 360
ggcgagttca tctacaaggt gaagctccgc ggcaccaact tcccctccga cggccccgta 420
atgcaaaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc 480
gccctcaagg gcgagatcaa gcaaaggctc aagctcaagg acggcggcca ctacgacgcc 540
gaggtgaaga ccacctacaa ggccaagaag cccgtgcaac tccccggcgc ctacaacgtg 600
aacatcaagc tcgacatcac ctcccacaac gaggactaca ccatcgtgga gcaatacgag 660
cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagtg a 711
<210> 33
<211> 1149
<212> DNA
<213> 人工合成
<400> 33
cgtcaacatg gtggagcacg acacgcttgt ctactccaaa aatatcaaag atacagtctc 60
agaagaccaa agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg 120
attccattgc ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc 180
ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag 240
tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac 300
cacgtcttca aagcaagtgg attgatgtga tgaattcaac cgtctcttcg tgagaataac 360
cgtggcctaa aaataagccg atgaggataa ataaaatgtg gtggtacagt acttcaagag 420
gtttactcat caagaggatg cttttccgat gagctctagt agtacatcgg acctcacata 480
cctccattgt ggtgaaatat tttgtgctca tttagtgatg ggtaaatttt gtttatgtca 540
ctctaggttt tgacatttca gttttgccac tcttaggttt tgacaaataa tttccattcc 600
gcggcaaaag caaaacaatt ttattttact tttaccactc ttagctttca caatgtatca 660
caaatgccac tctagaaatt ctgtttatgc cacagaatgt gaaaaaaaac actcacttat 720
ttgaagccaa ggtgttcatg gcatggaaat gtgacataaa gtaacgttcg tgtataagaa 780
aaaattgtac tcctcgtaac aagagacgga aacatcatga gacaatcgcg tttggaaggc 840
tttgcatcac ctttggatga tgcgcatgaa tggagtcgtc tgcttgctag ccttcgccta 900
ccgcccactg agtccgggcg gcaactacca tcggcgaacg acccagctga cctctaccga 960
ccggacttga atgcgctacc ttcgtcagcg acgatggccg cgtacgctgg cgacgtgccc 1020
ccgcatgcat ggcggcacat ggcgagctca gaccgtgcgt ggctggctac aaatacgtac 1080
cccgtgagtg ccctagctag aaacttacac ctgcaactgc gagagcgagc gtgtgagtgt 1140
agccgagta 1149
<210> 34
<211> 310
<212> DNA
<213> 马铃薯(Solanum tuberosum)
<400> 34
ctagacttgt ccatcttctg gattggccaa cttaattaat gtatgaaata aaaggatgca 60
cacatagtga catgctaatc actataatgt gggcatcaaa gttgtgtgtt atgtgtaatt 120
actagttatc tgaataaaag agaaagagat catccatatt tcttatccta aatgaatgtc 180
acgtgtcttt ataattcttt gatgaaccag atgcatttca ttaaccaaat ccatatacat 240
ataaatatta atcatatata attaatatca attgggttag caaaacaaat ctagtctagg 300
tgtgttttgc 310
<210> 35
<211> 663
<212> DNA
<213> 长穗偃麦草(Elytrigia elongata)
<400> 35
atggagagat cccggcgcct gctgctcgtg gcgggcctgc tggcggcgct gctcccggcg 60
gcggcggccg cgttcgggcc gcagccgggg gcgccgtgcg agcccacgct gctggcgacg 120
caggtggcgc tcttctgcgc gcccgacatg cccaccgcgc agtgctgcga gcccgtcgtc 180
gccgccgtcg acctcggcgg cggcgtcccc tgcctctgcc gcgtcgccgc ggagccgcag 240
ctcgtcatgg cgggcctcaa cgccacccac ctcctcacgc tctacagctc ctgcggcggc 300
ctccgccccg gcggcgccca cctcgccgcc gcctgcgaag gacccgctcc cccggccgcc 360
gtcgtcagca gccccccacc cccgccaccg tccgccgcac ctcgccgcaa gcagccagcg 420
cacgacgcac cgccaccgcc gccgtcgacc gagaagccag cgtccccgcc gccccaggag 480
cacgacggcg ccgccccccg cgccaaggcc gcccccgccc aggcggctac ctccccgctc 540
gcgcccgctg ccgccaccgc cccgccgccc caggcgccgc actccgccgc ggcgacggcg 600
tcgtccaagg cggccttctt cttcgtcgcc acggccatgc tcggcctcta catcatcctc 660
tga 663