CN107267399A - A kind of good lucidum strain rejuvenation method of mycelium growth vigor - Google Patents
A kind of good lucidum strain rejuvenation method of mycelium growth vigor Download PDFInfo
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Abstract
Prepare, once cultivate and second incubation the invention discloses a kind of good lucidum strain rejuvenation method of mycelium growth vigor, including purification, strain.The inventive method carries out rejuvenation, and mycelial growth state is good, and obtained mycelia whiteness, tight ness rating and growing way is good.
Description
Technical field
The present invention relates to a kind of lucidum strain rejuvenation method, the good ganoderma lucidum rejuvenation method of particularly a kind of mycelium growth vigor.
Background technology
Ganoderma lucidum is the drying fructification of On Polyporaceae red sesame or purple sesame.Ganoderma lucidum is a kind of precious medicinal fungi, category
In Basidiomycetes Polyporaceae Ganoderma, there is the medicinal history of more than 2,000 years in China.Ganoderma lucidum first recorded in《Sheng Nong's herbal classic》, the successive dynasties
Doctor thinks that ganoderma lucidum can treat a variety of diseases, is the precious medicine that strengthening by means of tonics is strengthened the body resistance to consolidate the constitution.GL-B be in ganoderma lucidum most
One of effective composition, is present in the fructification of ganoderma lucidum, conidia powder and mycelium, with suppression tumour, enhancing body to certainly
The ability removed by base, thus can reduce free radical to the damage of body, have the effect of anti-aging, can also improve immunity,
Anti-inflammatory, reducing blood lipid, hypoglycemic etc. are acted on.
As the health-care efficacy of ganoderma lucidum is widely recognized and received by people, Wild ganoderma far can not meet people's
Demand.Culture medium is ganoderma lucidum conditions on which persons or things depend for existence, different culture raw materials and with can to the mycelial growth of ganoderma lucidum, yield and
Active component etc. produces important influence.Wherein, quality influence of the rejuvenation culture medium on the finished product ganoderma lucidum in later stage is very big.It is existing
Most of culture medium used comprises only glucose, potato, peptone etc. in rejuvenation method.The culture medium nutrition being so made is not
It is sufficient so that out of order, obtained mycelia whiteness, tight ness rating and growing way is bad for mycelial growth during rejuvenation.
The content of the invention
The purpose of the present invention, is to provide a kind of ganoderma lucidum rejuvenation method.The inventive method carries out rejuvenation, mycelial growth state
Good, obtained mycelia whiteness, tight ness rating and growing way is good.
What the present invention was realized in.
A kind of good lucidum strain rejuvenation method of mycelium growth vigor, including prepared by purification, strain, once culture and secondary training
Support.
The good lucidum strain rejuvenation method of foregoing mycelium growth vigor, specifically includes following steps：
(1) purify：Test tube is with after 70-80% alcohol disinfecting, and wiped clean is placed in 1,000,000 grades of superclean benches, with purple
Outer lamp sterilizes 25-35min, loads culture medium, and the charge weight of culture medium is the 2/3 of test tube volume, takes zero godmother to plant mycelial growth
The mycelia fritter in 1-2cm, is put into culture medium foremost, covers mycelia fritter with culture medium, cladding thickness is 1-1.5cm,
Test tube plug is filled in, is put into 25-28 DEG C of insulating box and carries out culture 5-7 days, obtain lucid ganoderma stock culture；
(2) preparation of lucidum strain：Lucid ganoderma stock culture continues to cultivate 10-15 days, obtains lucidum strain；
(3) once cultivate：Corn flour is taken, adds water and mixes the wet water content for causing corn flour for 50-60% corn flour culture
Base, lucidum strain is placed in millet powder culture medium, after being cultivated 8-12 days at 20-30 DEG C, is obtained and is once cultivated lucidum strain；
(4) second incubation：Once culture lucidum strain is transferred in the test tube equipped with culture medium, test tube plug is filled in, in 25-
28 DEG C of cultures, culture is stopped when mycelial growth full test tube, you can.
The good lucidum strain rejuvenation method of foregoing mycelium growth vigor, the culture medium is calculated by weight, mainly by grape
Sugared 15-25 parts, 3-5 parts of yeast extract, 180-220 parts of potato, 1.5-2.5 parts of peptone, 15-25 parts of seawood meal, centipede powder 0.3-
5-15 parts of 0.5 part, 10-20 parts of frutus cnidii, 1-10 parts of chrysanthemum indicum, 1-10 parts of garlic and nutgrass flatsedge are made.
The good lucidum strain rejuvenation method of foregoing mycelium growth vigor, the culture medium is calculated by weight, mainly by grape
Sugared 18-22 parts, 3-5 parts of yeast extract, 190-210 parts of potato, 1.8-2.2 parts of peptone, 18-22 parts of seawood meal, centipede powder 0.3-
8-12 parts of 0.5 part, 13-17 parts of frutus cnidii, 3-7 parts of chrysanthemum indicum, 3-7 parts of garlic and nutgrass flatsedge are made.
The good lucidum strain rejuvenation method of foregoing mycelium growth vigor, the culture medium is calculated by weight, mainly by grape
20 parts of sugar, 4 parts of yeast extract, 200 parts of potato, 2.0 parts of peptone, 20 parts of seawood meal, 0.4 part of centipede powder, 15 parts of frutus cnidii, mother chrysanthemum
Spend 5 parts, 10 parts of 5 parts of garlic and nutgrass flatsedge be made.
The good lucidum strain rejuvenation method of foregoing mycelium growth vigor, the culture medium is prepared according to the following steps：
(1) frutus cnidii, chrysanthemum indicum, garlic and nutgrass flatsedge are taken, plus 6-8 times is measured water, decocts 2-3h, filtering is taken after filter residue drying,
Fine grained is ground into, A product are obtained；
(2) potato slice, plus 5-7 times measured water and boiled 20-30 minutes, is filtered, filter residue adds 5-7 times to measure water continuation, is heated to
50-60 DEG C, obtain B product；
(3) glucose, peptone, yeast extract, centipede powder, A product and seawood meal are sequentially added in B product, it is stirring while adding, stir
After even, C product are obtained；
(4) C product are dispensed to the 1/5 of 180ml test tubes, and silica gel plug sealing, 6-8 draws newspaper and wrapped, sterilized at 120-122 DEG C
After 20-30min, 50-60 DEG C is cooled to, 5-15 degree is tilted and is positioned in 20-35 DEG C of baking oven, after placing 24-48 hours, take
Go out, produce.
The good lucidum strain rejuvenation method of foregoing mycelium growth vigor, the culture medium is prepared according to the following steps：
(1) frutus cnidii, chrysanthemum indicum, garlic and nutgrass flatsedge, plus 7 times of amount water are taken, 2.5h is decocted, filtering is taken after filter residue drying, powder
Fine grained is broken into, A product are obtained；
(2) potato slice, plus 6 times of amount water boil 25 minutes, filter, and filter residue adds 6 times of amount water to continue, and is heated to 50-60 DEG C,
Obtain B product；
(3) glucose, peptone, yeast extract, centipede powder, A product and seawood meal are sequentially added in B product, it is stirring while adding, stir
After even, C product are obtained；
(4) C product are dispensed to the 1/5 of 180ml test tubes, silica gel plug sealing, and 7, which draw newspaper, wraps, and is sterilized at 120-122 DEG C
After 25min, 50-60 DEG C is cooled to, 10 degree is tilted and is positioned in 20-35 DEG C of baking oven, after placing 36 hours, takes out, produces.
Applicant has carried out substantial amounts of research to ganoderma lucidum rejuvenation method, and part Experiment is as follows：
Experimental example mycelial growths situation is investigated
1 material
1.1 ganoderma lucidum：There is provided by edible mushroom research institute of Shouguang City of Shandong Province.
1.2 contrast culture mediums：
Raw material：Glucose 20kg, yeast extract 4kg, potato 200kg, peptone 2.0kg, seawood meal 20kg and centipede powder
0.4kg。
Manufacture craft：
(1) potato slice, plus 6 times of amount water boil 25 minutes, filter, and filter residue adds 6 times of amount water to continue, and is heated to 50-60 DEG C,
Obtain A product；
(2) glucose, peptone, yeast extract, centipede powder and seawood meal are sequentially added in A product, it is stirring while adding, stir evenly
Afterwards, B product are obtained；
(3) B product are dispensed to the 1/5 of 180ml test tubes, silica gel plug sealing, and 7, which draw newspaper, wraps, and is sterilized at 120-122 DEG C
After 25min, 50-60 DEG C is cooled to, 10 degree is tilted and is positioned in 20-35 DEG C of baking oven, after placing 36 hours, takes out, produces.
2 test methods and result
Ganoderma lucidum is divided into 2 groups, of the present invention group and control group, present invention group carries out rejuvenation by embodiment 1.Control group rejuvenation side
Method be the same as Example 1, but when preparation and the second incubation of lucidum strain, culture medium used is above-mentioned contrast culture medium.During
The growing state of mycelia is observed, and is noted down.It the results are shown in Table 1 and table 2.
Mycelia climbs wall situation in the different rejuvenation culture mediums of table 1
The growth form of table 2 observes result
| Group | Mycelia whiteness | Tight ness rating | Mycelium growth vigor |
| Of the present invention group | It is pure white | Closely | It is very vigorous |
| Control group | Dark brightness | Closely | It is vigorous |
As seen from table, the Ganoderma lucidum mycelium growing way that the inventive method is carried out after rejuvenation is good, and growth form is carried out better than control group
The mycelia that rejuvenation is obtained.
Compared with prior art, rejuvenation is carried out with the inventive method, mycelial growth state is good, obtained mycelia whiteness,
Tight ness rating and growing way are good.
Embodiment
Embodiment 1.
Culture medium raw material：Glucose 20kg, yeast extract 4kg, potato 200kg, peptone 2.0kg, seawood meal 20kg, centipede
Powder 0.4kg, frutus cnidii 15kg, chrysanthemum indicum 5kg, garlic 5kg and nutgrass flatsedge 10kg.
The preparation method of culture medium：Specifically include following steps：
(1) frutus cnidii, chrysanthemum indicum, garlic and nutgrass flatsedge, plus 7 times of amount water are taken, 2.5h is decocted, filtering is taken after filter residue drying, powder
Fine grained is broken into, A product are obtained；
(2) potato slice, plus 6 times of amount water boil 25 minutes, filter, and filter residue adds 6 times of amount water to continue, and is heated to 50-60 DEG C,
Obtain B product；
(3) glucose, peptone, yeast extract, centipede powder, A product and seawood meal are sequentially added in B product, it is stirring while adding, stir
After even, C product are obtained；
(4) C product are dispensed to the 1/5 of 180ml test tubes, silica gel plug sealing, and 7, which draw newspaper, wraps, and is sterilized at 120-122 DEG C
After 25min, 50-60 DEG C is cooled to, 10 degree is tilted and is positioned in 20-35 DEG C of baking oven, after placing 36 hours, takes out, produces.
Rejuvenation of spawn method：Specifically include following steps：
(1) purify：Test tube is with after 75% alcohol disinfecting, and wiped clean is placed in 1,000,000 grades of superclean benches, with ultraviolet
Lamp sterilizes 30min, loads culture medium, and the charge weight of culture medium is the 2/3 of test tube volume, takes zero godmother to plant mycelial growth foremost
Mycelia fritter in 1-2cm, is put into culture medium, covers mycelia fritter with culture medium, cladding thickness is 1-1.5cm, fills in examination
Pipe close, is put into 25-28 DEG C of insulating box and carries out culture 6 days, obtain lucid ganoderma stock culture；
(2) preparation of lucidum strain：Lucid ganoderma stock culture continues to cultivate 13 days, obtains lucidum strain；
(3) once cultivate：Take corn flour, add water mix it is wet cause corn flour water content be 55% maize powder medium,
Lucidum strain is placed in millet powder culture medium, after being cultivated 10 days at 20-30 DEG C, obtains and once cultivates lucidum strain；
(4) second incubation：Once culture lucidum strain is transferred in the test tube equipped with culture medium, test tube plug is filled in, in 25-
28 DEG C of cultures, culture is stopped when mycelial growth full test tube, you can.
Embodiment 2.
Culture medium raw material：Glucose 25kg, yeast extract 5kg, potato 220kg, peptone 2.5kg, seawood meal 25kg, centipede
Powder 0.5kg, frutus cnidii 20kg, chrysanthemum indicum 10kg, garlic 10kg and nutgrass flatsedge 15kg.
Culture medium preparation method：
(1) frutus cnidii, chrysanthemum indicum, garlic and nutgrass flatsedge, plus 8 times of amount water are taken, 3h is decocted, filtering takes after filter residue drying, crushed
Into fine grained, A product are obtained；
(2) potato slice, plus 7 times of amount water boil 30 minutes, filter, and filter residue adds 7 times of amount water to continue, and is heated to 50-60 DEG C,
Obtain B product；
(3) glucose, peptone, yeast extract, centipede powder, A product and seawood meal are sequentially added in B product, it is stirring while adding, stir
After even, C product are obtained；
(4) C product are dispensed to the 1/5 of 180ml test tubes, silica gel plug sealing, and 8, which draw newspaper, wraps, and is sterilized at 120-122 DEG C
After 30min, 50-60 DEG C is cooled to, 15 degree is tilted and is positioned in 20-35 DEG C of baking oven, after placing 48 hours, takes out, produces.
Rejuvenation of spawn method：Specifically include following steps：
(1) purify：Test tube is with after 80% alcohol disinfecting, and wiped clean is placed in 1,000,000 grades of superclean benches, with ultraviolet
Lamp sterilizes 35min, loads culture medium, and the charge weight of culture medium is the 2/3 of test tube volume, takes zero godmother to plant mycelial growth foremost
Mycelia fritter in 1-2cm, is put into culture medium, covers mycelia fritter with culture medium, cladding thickness is 1-1.5cm, fills in examination
Pipe close, is put into 25-28 DEG C of insulating box and carries out culture 7 days, obtain lucid ganoderma stock culture；
(2) preparation of lucidum strain：Lucid ganoderma stock culture continues to cultivate 15 days, obtains lucidum strain；
(3) once cultivate：Take corn flour, add water mix it is wet cause corn flour water content be 60% maize powder medium,
Lucidum strain is placed in millet powder culture medium, after being cultivated 12 days at 20-30 DEG C, obtains and once cultivates lucidum strain；
(4) second incubation：Once culture lucidum strain is transferred in the test tube equipped with culture medium, test tube plug is filled in, in 25-
28 DEG C of cultures, culture is stopped when mycelial growth full test tube, you can.
Embodiment 3.
Culture medium raw material：Glucose 15kg, yeast extract 3kg, potato 180kg, peptone 1.5kg, seawood meal 15kg, centipede
Powder 0.3kg, frutus cnidii 10kg, chrysanthemum indicum 1kg, garlic 1kg and nutgrass flatsedge 5kg.
Culture medium preparation method：
(1) frutus cnidii, chrysanthemum indicum, garlic and nutgrass flatsedge, plus 6 times of amount water are taken, 2h is decocted, filtering takes after filter residue drying, crushed
Into fine grained, A product are obtained；
(2) potato slice, plus 5 times of amount water boil 20 minutes, filter, and filter residue adds 5 times of amount water to continue, and is heated to 50-60 DEG C,
Obtain B product；
(3) glucose, peptone, yeast extract, centipede powder, A product and seawood meal are sequentially added in B product, it is stirring while adding, stir
After even, C product are obtained；
(4) C product are dispensed to the 1/5 of 180ml test tubes, and silica gel plug sealing, 6-8 draws newspaper and wrapped, sterilized at 120-122 DEG C
After 20min, 50-60 DEG C is cooled to, 5 degree is tilted and is positioned in 20-35 DEG C of baking oven, after placing 24 hours, takes out, produces.
Rejuvenation of spawn method：Specifically include following steps：
(1) purify：Test tube is with after 70% alcohol disinfecting, and wiped clean is placed in 1,000,000 grades of superclean benches, with ultraviolet
Lamp sterilizes 25min, loads culture medium, and the charge weight of culture medium is the 2/3 of test tube volume, takes zero godmother to plant mycelial growth foremost
Mycelia fritter in 1-2cm, is put into culture medium, covers mycelia fritter with culture medium, cladding thickness is 1-1.5cm, fills in examination
Pipe close, is put into 25-28 DEG C of insulating box and carries out culture 5 days, obtain lucid ganoderma stock culture；
(2) preparation of lucidum strain：Lucid ganoderma stock culture continues to cultivate 10 days, obtains lucidum strain；
(3) once cultivate：Take corn flour, add water mix it is wet cause corn flour water content be 50% maize powder medium,
Lucidum strain is placed in millet powder culture medium, after being cultivated 8 days at 20-30 DEG C, obtains and once cultivates lucidum strain；
(4) second incubation：Once culture lucidum strain is transferred in the test tube equipped with culture medium, test tube plug is filled in, in 25-
28 DEG C of cultures, culture is stopped when mycelial growth full test tube, you can.
Claims (7)
1. a kind of good lucidum strain rejuvenation method of mycelium growth vigor, it is characterised in that：Prepare, once cultivate including purification, strain
And second incubation.
2. the good lucidum strain rejuvenation method of mycelium growth vigor according to claim 1, it is characterised in that：Specifically include following
Step：
（1）Purification：Test tube is with after 70-80% alcohol disinfecting, and wiped clean is placed in 1,000,000 grades of superclean benches, uses uviol lamp
25-35min is sterilized, loads culture medium, the charge weight of culture medium is the 2/3 of test tube volume, before taking zero godmother kind mycelial growth most
The mycelia fritter in 1-2cm is held, is put into culture medium, mycelia fritter is covered with culture medium, cladding thickness is 1-1.5cm, is filled in
Test tube plug, is put into 25-28 DEG C of insulating box and carries out culture 5-7 days, obtain lucid ganoderma stock culture；
（2）The preparation of lucidum strain：Lucid ganoderma stock culture continues to cultivate 10-15 days, obtains lucidum strain；
（3）Once cultivate：Corn flour is taken, adds water and mixes the wet water content for causing corn flour for 50-60% maize powder medium, will
Lucidum strain is placed in millet powder culture medium, after being cultivated 8-12 days at 20-30 DEG C, is obtained and is once cultivated lucidum strain；
（4）Second incubation：Once culture lucidum strain is transferred in the test tube equipped with culture medium, test tube plug is filled in, in 25-28
DEG C culture, culture is stopped when mycelial growth full test tube, you can.
3. the good lucidum strain rejuvenation method of mycelium growth vigor as claimed in claim 2, it is characterised in that：The culture medium is by weight
Measure part to calculate, mainly by 15-25 parts of glucose, 3-5 parts of yeast extract, 180-220 parts of potato, 1.5-2.5 parts of peptone, seawood meal
5-15 parts of 15-25 parts, 0.3-0.5 parts of centipede powder, 10-20 parts of frutus cnidii, 1-10 parts of chrysanthemum indicum, 1-10 parts of garlic and nutgrass flatsedge are made
Into.
4. the good lucidum strain rejuvenation method of mycelium growth vigor as claimed in claim 3, it is characterised in that：The culture medium is by weight
Measure part to calculate, mainly by 18-22 parts of glucose, 3-5 parts of yeast extract, 190-210 parts of potato, 1.8-2.2 parts of peptone, seawood meal
8-12 parts of 18-22 parts, 0.3-0.5 parts of centipede powder, 13-17 parts of frutus cnidii, 3-7 parts of chrysanthemum indicum, 3-7 parts of garlic and nutgrass flatsedge are made.
5. the good lucidum strain rejuvenation method of mycelium growth vigor as claimed in claim 4, it is characterised in that：The culture medium is by weight
Measure part to calculate, mainly by 20 parts of glucose, 4 parts of yeast extract, 200 parts of potato, 2.0 parts of peptone, 20 parts of seawood meal, centipede powder
10 parts of 0.4 part, 15 parts of frutus cnidii, 5 parts of chrysanthemum indicum, 5 parts of garlic and nutgrass flatsedge are made.
6. the good lucidum strain rejuvenation method of mycelium growth vigor as any one of claim 4-5, it is characterised in that：It is described
Culture medium is prepared according to the following steps：
（1）Frutus cnidii, chrysanthemum indicum, garlic and nutgrass flatsedge are taken, plus 6-8 times is measured water, decocts 2-3h, filtering takes after filter residue drying, crushed
Into fine grained, A product are obtained；
（2）Potato slice, plus 5-7 times measured water and boiled 20-30 minutes, is filtered, filter residue adds 5-7 times to measure water continuation, is heated to 50-60
DEG C, obtain B product；
（3）Glucose, peptone, yeast extract, centipede powder, A product and seawood meal are sequentially added in B product, it is stirring while adding, stir evenly
Afterwards, C product are obtained；
（4）C product are dispensed to the 1/5 of 180ml test tubes, and silica gel plug sealing, 6-8 draws newspaper and wrapped, and sterilize 20- at 120-122 DEG C
After 30min, 50-60 DEG C is cooled to, 5-15 degree is tilted and is positioned in 20-35 DEG C of baking oven, after placing 24-48 hours, is taken out, i.e.,
.
7. the good lucidum strain rejuvenation method of mycelium growth vigor as claimed in claim 6, it is characterised in that：The culture medium is pressed
State step preparation：
（1）Frutus cnidii, chrysanthemum indicum, garlic and nutgrass flatsedge, plus 7 times of amount water are taken, 2.5h is decocted, filtering takes after filter residue drying, is ground into
Fine grained, obtains A product；
（2）Potato slice, plus 6 times of amount water boil 25 minutes, filter, and filter residue adds 6 times of amount water to continue, and is heated to 50-60 DEG C, obtains B
Product；
（3）Glucose, peptone, yeast extract, centipede powder, A product and seawood meal are sequentially added in B product, it is stirring while adding, stir evenly
Afterwards, C product are obtained；
（4）C product are dispensed to the 1/5 of 180ml test tubes, silica gel plug sealing, and 7, which draw newspaper, wraps, and sterilize 25min at 120-122 DEG C
Afterwards, 50-60 DEG C is cooled to, 10 degree is tilted and is positioned in 20-35 DEG C of baking oven, after placing 36 hours, takes out, produces.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104956914A (en) * | 2015-06-18 | 2015-10-07 | 唐伟 | Breeding method for natural ganoderma lucidum |
| CN105367316A (en) * | 2015-12-24 | 2016-03-02 | 桂林大野领御生物科技有限公司 | Lucid ganoderma culture medium |
-
2017
- 2017-06-20 CN CN201710471065.8A patent/CN107267399A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104956914A (en) * | 2015-06-18 | 2015-10-07 | 唐伟 | Breeding method for natural ganoderma lucidum |
| CN105367316A (en) * | 2015-12-24 | 2016-03-02 | 桂林大野领御生物科技有限公司 | Lucid ganoderma culture medium |
Non-Patent Citations (2)
| Title |
|---|
| 丁安伟等: "《中药资源综合利用与产品开发》", 30 April 2013 * |
| 张真等: "《"浙八味"中药材规范化生产技术》", 31 December 2007 * |
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