CN106497795A - A kind of Cordyceps funguss seed culture medium containing Fructus Lycii and its application - Google Patents
A kind of Cordyceps funguss seed culture medium containing Fructus Lycii and its application Download PDFInfo
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Abstract
The present invention relates to a kind of Cordyceps funguss seed culture medium containing Fructus Lycii, contains Fructus Lycii 2～8g/L or 2～8g/kg in the culture medium；The Cordyceps funguss are preferably phorozoon China Mortierella, epigamouss China Mortierella, Cordycepses cephalo is mould, curved neck is mould, synnema is mould or peacilomyce hepiahi.The invention further relates to application of the seed culture medium in culture Cordyceps funguss.The Cordyceps funguss seed culture medium raw material for providing of the invention is simple, convenience of drawing materials, and required Fructus Lycii is few, low cost；Strain mycelium with the culture medium culturing is strong, fast growth, routinely fermentation condition transferred species, in the mycelium that obtains, cordycepin content is high after fermentation；The strain of the bacterium culture medium culture gained provided with the present invention, can be used to produce Cordycepses class health product.
Description
Technical field
The present invention relates to microorganism culturing, fermentation arts, and in particular to a kind of Cordyceps funguss seed containing Fructus Lycii
Culture medium and its application.
Background technology
Cordyceps medicinal fungi is the famous and precious wild Chinese crude drug of a class of China, and Cordycepses class known to the whole world has more than 350 kinds, I
State has 62 kinds at present, and wherein, but most medical value is Cordyceps funguss.
Cordyceps are a kind of famous and precious, the rare Chinese medicine of China, and medication is with a long history, with Radix Ginseng, Cornu Cervi Pantotrichum three big taste arranged side by side
The diseases such as one of tonic, chronic cough empty for nourishing lung and kidney two, bronchitis, sexual impotence, menoxenia, hyperlipidemia, heart blood supply insufficiency,
There is the pharmacological actions such as antitumor, enhancing immunity, antiviral, antifungal, scientific research to find that Cordyceps contain adenosine, worm
The pharmaceutical chemistry materials such as careless element, cordycepic acid, polysaccharide, are that Cordyceps play drug effect, and formulate Cordyceps quality standard
Important substance basis, in recent years, domestic and international scientist has found, cordycepin can disturb the synthesis of gene fine RNA and DNA, suppresses
The division of the abnormal cell such as cancerous cell, shows very strong antifungal, anti-HlV-I types virus and suppresses clostridium activity, as
Anticancer, new antiviral drug, cordycepin the U.S. oneself by the safety evaluation of FDA, and enter into three phases clinic valency section.
Cordyceps funguss are isolated a kind of medicinal true from the polypide of fresh, sterilized Cordyceps or Stroma
Bacterium, with the deep fermentation Cordyceps mycelium that obtains of production, containing with wild cordyceps identical ingredient, with the worm summer in winter
Careless mycelium is obtained multiple product, and such as JINSHUIBAO, lark capsule etc. is accommodated in Chinese Pharmacopoeia as first class national new drug, has
Good market prospect.During the last ten years, domestic many units, although to the growth characteristics of Cordyceps funguss, fermentation culture
Base optimization and fermentation technology carry out numerous studies work, and Cordyceps mycelium active component is relatively low, particularly Cordyceps funguss
In filament, cordycepin content is lower, it is therefore necessary to improve the cordycepin content of Cordyceps funguss.The existing skill for improving cordycepin
Art scheme is usually that fermentation medium is improved, or adds some chemical substances, such as Oleic acid or its precursor in sweat
Thing analog or plant growth regulator wait until in fermentation medium that, as fermentation medium volume is big, required raw material adds
Plus the weight of thing is big, high cost.Seldom see by the research to Cordyceps funguss seed culture medium, improve cordycepin content
Research.Therefore, the innovation of Cordyceps fungus zymocyte seed culture medium technical research, to improving fermentation Cordyceps funguss cordycepin
Content, the product quality and medicinal effects for improving Cordyceps funguss have positive effect.
Fructus Lycii, is the fruit of the medicine-food two-purpose of Solanaceae Thorny plants, with Changbai Mountain Radix Ginseng, Donga Colla Corii Asini, fine jade treasure Ganoderma
And claim Chinese medicine four precious, early in《Sheng Nong's herbal classic》,《Compendium of Materia Medica》In on the books.Modern study shows that Fructus Lycii contains greatly
The lycium barbarum polysaccharide of amount and glycine betaine, with work(such as promotion immunity, antioxidation, defying age, resisting fatigue, radioprotective, antitumor, liver protections
Effect.At present, having not seen, Fructus Lycii is used for the relevant report of edible funguses culture.
Content of the invention
It is an object of the invention to overcoming the defect of prior art, there is provided a kind of Cordyceps funguss seed containing Fructus Lycii
Culture medium.
Specifically, in the Cordyceps funguss seed culture medium that the present invention is provided, containing Fructus Lycii 2～8g/L or 2～8g/
kg.
Fructus Lycii of the present invention refers to the mature fruit of matrimony vine of solanaceae plant.
Cordyceps funguss of the present invention refer to isolated from the polypide of fresh, sterilized Cordyceps or Stroma
Medicinal fungi；Phorozoon China Mortierella preferably well known in the art, epigamouss China Mortierella, Cordycepses cephalo are mould, curved
Neck is mould, synnema is mould or peacilomyce hepiahi.
By a large amount of practices, the present invention has found that the incubation that Fructus Lycii is used for Cordyceps funguss can be significantly improved
The quality of Cordyceps funguss, it is particularly possible to put forward the content of cordycepin in Cordyceps funguss, so that improve its medical value.
Also include carbon source, nitrogen source and inorganic salt in the Cordyceps funguss seed culture medium.The carbon source, nitrogen source and
Inorganic salt can select the conventional component in this area.Specifically, the carbon source can select Rhizoma Solani tuber osi or/and oatmeal and Portugal
Grape sugar or/and sucrose, the nitrogen source can select peptone and yeast extract；The inorganic salt can select potassium dihydrogen phosphate and sulfur
Sour magnesium.
In order to ensure synergism between each component, further improve product quality comprehensively, the preferably described seed of the present invention
Composition comprising following weight portion in culture medium：30～200 parts of 2.0～8.0 parts of Fructus Lycii, Rhizoma Solani tuber osi or/and oatmeal, Fructus Vitis viniferae
16～21 parts of sugar or/and sucrose, 3.0～5.0 parts of peptone, 2.0～3.0 parts of yeast extract, 0.6～1.0 part of potassium dihydrogen phosphate, sulfur
0.3～0.5 part of sour magnesium.
Used as a preferred embodiment of the present invention, the seed culture medium is fluid medium, wherein includes following composition：
2～8g/L of Fructus Lycii, 30～200g/L of Rhizoma Solani tuber osi or/and oatmeal, 15～21g/L of glucose or/and sucrose, peptone 3～
5g/L, 2～3g/L of yeast extract, 0.6～1g/L of potassium dihydrogen phosphate, 0.3～0.5g/L of magnesium sulfate, distilled water surplus.
It is highly preferred that including following composition in the seed culture medium：4.0～5.0g/L of Fructus Lycii, Rhizoma Solani tuber osi 150～
200g/L, 15～20g/L of glucose, 3～4g/L of peptone, 1.5～2.5g/L of yeast extract, 0.6～1.0g/ of potassium dihydrogen phosphate
L, 0.3～0.5g/L of magnesium sulfate, distilled water surplus.
The present invention provide Cordyceps funguss seed culture medium, can routinely culture medium compound method prepare.Such as will be upper
State quality proportioning and weigh raw material, be put into container, heat, dissolving, routinely sterilization method after mix homogeneously.
The present invention further protects application of the seed culture medium in culture Cordyceps funguss.
Specifically, the seed culture medium can be used for the culture of Cordyceps funguss seed, is then trained with the seed
The seed liquor that foster base culture is obtained is inoculated in Cordyceps bacteria fermentation culture medium is fermented, so as to significantly improve fermentation liquid and
Cordycepin content in fermentation gained mycelium.
Described fermentation liquid further can be prepared into Chinese caterpillar fungus oral liquid by the present invention, or in described fermentation liquid
Pure grain rice wine is added, Chinese caterpillar fungus health-care original plasm wine is prepared into.
The Cordyceps bacteria fermentation culture medium is the conventional fermentation medium in this area.Utilizing the seed culture medium
On the basis of being cultivated, in order to further improve the quality of product, the preferably described Cordyceps bacteria fermentation culture medium of the present invention
Consist of：10～20g/L of glucose, 5～10g/L of sucrose, 5～15g/L of Semen setariae, 5～10g/L of peptone, soybean cake powder 10～
20g/L, 2～3g/L of yeast extract, 0.5～1g/L of potassium dihydrogen phosphate, 0.1～1g/L of magnesium sulfate, vitaminB10 .01～
0.05g/L.
The specific seed culture method of Cordyceps funguss described above and fermentation process are this area routine operation, this
Particular determination is not done in invention.
The present invention is creatively applied to Fructus Lycii in the seed culture and fermentation of Cordyceps funguss.The present invention is provided
Cordyceps funguss seed culture medium raw material is simple, convenience of drawing materials, and required Fructus Lycii is few, low cost；Trained with the seed culture medium
Foster seed growth speed is fast, quality is good, routinely fermentation condition transferred species, cordycepin content in the mycelium that obtains after fermentation
High；The product of the seed culture medium culture gained provided with the present invention, can be used for the production of medicine and health product.
Specific embodiment
Following examples are used for the present invention to be described, but are not limited to the scope of the present invention.
In following embodiment, involved Cordyceps funguss are to gather the fresh winter worm summer from Cordyceps Sinensis From Tibet producing region
Grass, through over cleaning, surface sterilization, dials from crust, white polypide and is cut into grain of rice size block, be inoculated in improvement PDA culture medium,
15 degree are cultivated 20 days or so, and picking funguses further separate, obtain single culture；Cultural characteristic, microscope are carried out to the strain
Observation and RNA sequencings, are accredited as Cordyceps funguss.
Embodiment 1
The seed culture medium that the present embodiment is provided is consisted of：Fructus Lycii 2.5g/L, Rhizoma Solani tuber osi 200g/L, glucose 20g/L,
Peptone 3.5g/L, yeast extract 2.0g/L, potassium dihydrogen phosphate 0.6g/L, magnesium sulfate 0.3g/L, distilled water surplus.
Embodiment 2
The seed culture medium that the present embodiment is provided is consisted of：Fructus Lycii 4.0g/L, Rhizoma Solani tuber osi 200g/L, glucose 20g/L,
Peptone 4.0g/L, yeast extract 2.5g/L, potassium dihydrogen phosphate 0.8g/L, magnesium sulfate 0.4g/L, distilled water surplus.
Embodiment 3
A kind of Cordyceps funguss seed culture medium is present embodiments provided, consisting of：Fructus Lycii 5.5g/L, Rhizoma Solani tuber osi
200g/L, glucose 21g/L, peptone 4.0g/L, yeast extract 3.0g/L, potassium dihydrogen phosphate 1.0g/L, magnesium sulfate 0.5g/L, steam
Distilled water surplus.
Embodiment 4
The seed culture medium that the present embodiment is provided is consisted of：Fructus Lycii 6.0g/L, Rhizoma Solani tuber osi 200g/L, glucose 12g/L,
Sucrose 4g/L, peptone 5.0g/L, yeast extract 2.5g/L, potassium dihydrogen phosphate 1.0g/L, magnesium sulfate 0.5g/L, distilled water surplus.
Embodiment 5
The seed culture medium that the present embodiment is provided is consisted of：Fructus Lycii 7.0g/L, Rhizoma Solani tuber osi 200g/L, sucrose 16g/L, egg
White peptone 3.0g/L, yeast extract 2.5g/L, potassium dihydrogen phosphate 0.8g, magnesium sulfate 0.4g/L, distilled water surplus.
Embodiment 6
The seed culture medium that the present embodiment is provided is consisted of：Fructus Lycii 4.0g/L, oatmeal 30g/L, glucose 13g/L,
Sucrose 5g/L, peptone 5.0g/L, yeast extract 2.5g/L, potassium dihydrogen phosphate 0.7g, magnesium sulfate 0.5g/L, distilled water surplus.
Embodiment 7
Cordyceps strain is respectively connected to seed culture medium and the control seed culture medium that embodiment 1 is provided, is pressed respectively
Conventional method culture strain；Then, the strain through above-mentioned culture gained is accessed liquid fermentation medium respectively, according to a conventional method
Cultivation and fermentation, collects fermentation liquid.
Described control seed culture medium formula be：Rhizoma Solani tuber osi 200g/L, glucose 20g/L, peptone 3.5g/L, yeast
Cream 2.0g/L, potassium dihydrogen phosphate 0.6g/L, magnesium sulfate 0.3g/L, distilled water surplus.
The formula of the fermentation medium is：Glucose 15g/L, sucrose 7g/L, Semen setariae 10g/L, peptone 6g/L, soybean cake
Powder 15g/L, yeast extract 2.5g/L, potassium dihydrogen phosphate 0.8g/L, magnesium sulfate 0.5g/L, vitamin B1 0.03g/L, distilled water
Surplus.
Detected per the cordycepin content mg contained by mycelium in 100ml fermentation liquids, as a result such as by this area conventional method
Shown in table 1
Table 1：Cordycepin content in per 100ml fermentation liquids contained by mycelium
| Bacterium culture medium is originated | Cordycepin content (mg/100ml) |
| Control | 0.055 |
| Embodiment 1 | 0.153 |
Embodiment 8
Cordyceps strain is respectively connected to seed culture medium and the control seed culture medium that embodiment 2 is provided, is pressed respectively
Conventional method culture strain；Remaining operation and control seed culture medium formula and fermentation medium formula with embodiment 7,
Collect fermentation liquid.
Detected per the cordycepin content mg contained by mycelium in 100ml fermentation liquids, as a result such as by this area conventional method
Shown in table 2
Table 2：Cordycepin content in per 100ml fermentation liquids contained by mycelium
| Bacterium culture medium is originated | Cordycepin content (mg/100ml) |
| Control | 0.050 |
| Embodiment 2 | 0.210 |
Embodiment 9
Cordyceps strain is respectively connected to seed culture medium and the control seed culture medium that embodiment 3 is provided, is pressed respectively
Conventional method culture strain；Remaining operation and control seed culture medium formula and fermentation medium formula with embodiment 7,
Collect fermentation liquid.
Detected per the cordycepin content mg contained by mycelium in 100ml fermentation liquids, as a result such as by this area conventional method
Shown in table 3
Table 3：Cordycepin content in per 100ml fermentation liquids contained by mycelium
| Bacterium culture medium is originated | Cordycepin content (mg/100ml) |
| Control | 0.047 |
| Embodiment 3 | 0.263 |
Embodiment 10
Cordyceps strain is respectively connected to seed culture medium and the control seed culture medium that embodiment 4 is provided, is pressed respectively
Conventional method culture strain；Remaining operation and control seed culture medium formula and fermentation medium formula with embodiment 7,
Collect fermentation liquid.
By the fermentation liquid through homogenizer process, it is 1 by volume：The pure grain of 28 degree of the alcoholic strength that 1 ratio is added
Rice wine, obtains Cordycepses oleo stock health promoting wine after aging.
By content mg of cordycepin in the every 100ml Cordycepses oleo stock health promoting wine of this area conventional method detection, as a result such as table 4
Shown
Table 4：Cordycepin content in per 100ml Cordycepses oleo stock health promoting wine
| Bacterium culture medium is originated | Cordycepin content (mg/100ml) |
| Control | 0.024 |
| Embodiment 4 | 0.135 |
Embodiment 11
Cordyceps strain is respectively connected to seed culture medium and the control seed culture medium that embodiment 5 is provided, is pressed respectively
Conventional method culture strain；Remaining operation and control seed culture medium formula and fermentation medium formula with embodiment 7,
Collect fermentation liquid；
By the fermentation liquid through homogenizer process, it is 1 by volume：The pure grain of 28 degree of the alcoholic strength that 1 ratio is added
Rice wine, obtains Cordycepses oleo stock health promoting wine after aging.
By content mg of cordycepin in the every 100ml Cordycepses oleo stock health promoting wine of this area conventional method detection, as a result such as table 5
Shown
Table 5：Cordycepin content in per 100ml Cordycepses oleo stock health promoting wine
| Bacterium culture medium is originated | Cordycepin content (mg/100ml) |
| Control | 0.025 |
| Embodiment 5 | 0.124 |
Embodiment 12
Cordyceps strain is respectively connected to seed culture medium and the control seed culture medium that embodiment 6 is provided, is pressed respectively
Conventional method culture strain；Remaining operation and control seed culture medium formula and fermentation medium formula with embodiment 7,
Collect fermentation liquid；The fermentation liquid prepares Chinese caterpillar fungus oral liquid behind the 1/2 of membrance concentration to original volume.
By content mg of cordycepin in the every 100ml Chinese caterpillar fungus oral liquids of this area conventional method detection, as a result such as table 6
Shown
Table 6：Cordycepin content in per 100ml Chinese caterpillar fungus oral liquids
| Bacterium culture medium is originated | Cordycepin content (mg/100ml) |
| Control | 0.047 |
| Embodiment 6 | 0.388 |
Although, general explanation, specific embodiment and test has above been used, the present invention has been made to retouch in detail
State, but on the basis of the present invention, it can be made some modifications or improvements, this is apparent to those skilled in the art
's.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, belong to claimed
Scope.
Claims (10)
1. a kind of Cordyceps funguss seed culture medium containing Fructus Lycii, it is characterised in that contain Fructus Lycii 2 in the culture medium
～8g/L or 2～8g/kg；
The Cordyceps funguss be preferably phorozoon China Mortierella, epigamouss China Mortierella, Cordycepses cephalo is mould, curved neck is mould,
Synnema is mould or peacilomyce hepiahi.
2. culture medium according to claim 1, it is characterised in that also include carbon source, nitrogen source and nothing in the culture medium
Machine salt.
3. culture medium according to claim 2, it is characterised in that the carbon source selected from Rhizoma Solani tuber osi, oatmeal, glucose,
One or more in sucrose；
And/or, the nitrogen source is selected from one or two in peptone, yeast extract；
And/or, the inorganic salt is selected from one or two in potassium dihydrogen phosphate, magnesium sulfate.
4. culture medium according to claim 1, it is characterised in that the composition comprising following weight portion：Fructus Lycii 2.0～
8.0 parts, Rhizoma Solani tuber osi or/and 16～21 parts of 30～200 parts of oatmeal, glucose or/and sucrose, 3.0～5.0 parts of peptone, ferment
2.0～3.0 parts of female cream, 0.6～1.0 part of potassium dihydrogen phosphate, 0.3～0.5 part of magnesium sulfate.
5. culture medium according to claim 1, it is characterised in that the culture medium is fluid medium, comprising following into
Point：2～8g/L of Fructus Lycii, 30～200g/L of Rhizoma Solani tuber osi or/and oatmeal, 15～21g/L of glucose or/and sucrose, peptone 3
～5g/L, 2～3g/L of yeast extract, 0.6～1g/L of potassium dihydrogen phosphate, 0.3～0.5g/L of magnesium sulfate, distilled water surplus.
6. culture medium according to claim 5, it is characterised in that comprising following composition：4.0～5.0g/L of Fructus Lycii, horse
Bell 150～200g/L of potato, 15～20g/L of glucose, 3～4g/L of peptone, 1.5～2.5g/L of yeast extract, potassium dihydrogen phosphate
0.6～1.0g/L, 0.3～0.5g/L of magnesium sulfate, distilled water surplus.
7. application of the culture medium described in claim 1～6 any one in culture Cordyceps funguss.
8. application according to claim 7, it is characterised in that the Cordyceps funguss are phorozoon China Mortierella, have
Property type China Mortierella, Cordycepses cephalo is mould, curved neck is mould, synnema is mould or peacilomyce hepiahi.
9. application according to claim 8, it is characterised in that with the seed culture medium culture seed liquor；
Preferably, then the seed liquor is inoculated in fermentation medium and is fermented；
It is highly preferred that with the fermentation liquid for fermenting and obtaining, being further prepared into Chinese caterpillar fungus oral liquid or Cordyceps oleo stock
Health promoting wine.
10. application according to claim 9, it is characterised in that the fermentation medium is consisted of：Glucose 10～
20g/L, 5～10g/L of sucrose, 5～15g/L of Semen setariae, 5～10g/L of peptone, 10～20g/L of soybean cake powder, 2～3g/ of yeast extract
L, 0.5～1g/L of potassium dihydrogen phosphate, 0.1～1g/L of magnesium sulfate, 0.01～0.05g/L of vitamin B1.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107373340A (en) * | 2017-07-26 | 2017-11-24 | 锬酃藏虫草生物科技(深圳)有限公司 | A kind of selenium-rich cordyceps oat steamed bun and preparation method thereof |
| CN113317119A (en) * | 2021-06-30 | 2021-08-31 | 山东中医药大学 | Method for improving cordycepin content by virtue of Jiuzhou cordyceps sinensis-sculellaria barbata bidirectional solid fermentation |
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| CN101475927A (en) * | 2009-01-21 | 2009-07-08 | 孟繁宇 | Additive for Cordyceps militaris fluid nutrient medium |
| CN102199543A (en) * | 2011-01-19 | 2011-09-28 | 成都医学院 | New Poria cocos strain and liquid fermentation method thereof |
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