CN105359840A - Substitute cultivation technology for Ganoderma lucidum Antler - Google Patents
Substitute cultivation technology for Ganoderma lucidum Antler Download PDFInfo
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- CN105359840A CN105359840A CN201510982320.6A CN201510982320A CN105359840A CN 105359840 A CN105359840 A CN 105359840A CN 201510982320 A CN201510982320 A CN 201510982320A CN 105359840 A CN105359840 A CN 105359840A
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- ganoderma lucidum
- nutrient solution
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- sesame
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention discloses a substitute cultivation technology for Ganoderma lucidum Antler. The technology comprises steps as follows: early-stage processing of saw dust; batching, stirring and composting fermentation: batching is performed according to a formula of a culture medium, firstly, the saw dust, auxiliary materials, gypsum and sugar are mixed uniformly to form a dry material, a nutrient solution is added and stirred sufficiently and uniformly, a culture medium substrate is formed, composting fermentation is performed after stirring is finished, the nutrient solution is sufficiently absorbed by the culture medium substrate, and the product can be bagged; sterilization; inoculation; fruiting stage management; bud thinning stage management; harvesting: mature Ganoderma lucidum Antler is harvested in time. The special nutrient solution is adopted to control a material-water ratio, the nutritive value of the obtained Ganoderma lucidum Antler is high, and the polysaccharide content is substantially increased compared with the prior art. The utilized nutrient solution, especially utilized plant raw material composition, is subjected to steam explosion, distillation and processing of a high shear mixer, a nano-emulsion plant oil component is obtained and has good compatibility with other formula components in the culture medium, and proliferation of multiple kinds of cells can be stimulated.
Description
Technical field
The present invention relates to field of edible fungus culture, particularly relate to Ganoderma lucidum substituting stuff cultivation technique.
Background technology
Glossy ganoderma (formal name used at school: GanodermaLucidumKarst), also known as polyporus lucidus, celestial grass, have another name called red sesame, red sesame, Ganoderma Lucidum, Ganoderma, 10000 years gill fungus, polyporus lucidus, and being the complete stool of the red sesame of Polyporaceae plant or purple sesame, is the fruit body of On Polyporaceae glossy ganoderma.Profile is umbrella, cap kidney shape, semicircle or subcircular.Glossy ganoderma, as the Chinese tradition valuable ingredient of traditional Chinese medicine having thousands of years medicinal histories, has invigorating qi for tranquilization, relieving cough and asthma effect, and for dizzy egersis, palpitation, consumptive disease is coughed and breathed heavily.Glossy ganoderma possesses very high medical value, and the modern pharmacology research through scientific research institution's many decades confirms, glossy ganoderma, for enhancing body immunity, regulates blood sugar, controls blood pressure, adjuvant therapy chemicotherapy, liver protecting, promotes that the aspects such as sleep all have significant curative effect.GL-B is one of principle active component of glossy ganoderma medicinal function; mainly be present in glossy ganoderma cell wall inwall; major part is beta glucan; its monose composition is except containing except a large amount of glucose; also containing a small amount of arabinose, wood sugar, fucose; rhamnose; galactose and mannose etc.; they are connected with glycosidic bonds such as (1-6) mainly with (1-3), (1-4) greatly; there is biologically active widely, as develop immunitypty, remove human free radical, protect cardiovascular, radioresistance, protection liver, the effect such as antitumor.Triterpenes is also one of active ingredient of glossy ganoderma, and triterpenes contained by glossy ganoderma not lower over one hundred kind, wherein based on tetracyclic triterpenes, the bitter taste of glossy ganoderma is relevant with contained triterpenes, to human hepatoma cell, there is cytotoxicity, also can suppress the release of histamine, have and protect the liver and the effects such as antiallergy.
Ganoderma lucidum is that likeness in form has the glossy ganoderma of a pair as deer horn body, it is undifferentiated cap in process of growth, be different from the pileus ganoderma lucidum that other are general, do not distribute conidial powder, therefore do not consume maternal nutrition, nutrition is retained in fruit body completely, so the effective ingredient such as polysaccharide, ergosterol, mannitol, adenosine, triamido acid that Antler Mythic Fungus sporophore contains is higher than other common pileus ganoderma lucidum content, be the superfine product in glossy ganoderma, indices content is all considerably beyond sheet sesame.Such as, can improve the content of the organic germanium of human body cell oxidation resistance up to 1680 to 2000ppm, be 3 to 4 times of sheet sesame, the contained macromolecule polysaccharide body that can strengthen immune function of human body is 43%, is more than 5 times of sheet sesame.Not only medical value is high, and Ganoderma lucidum and sheet sesame are carried out live body grafting, cultivates glossy ganoderma dish garden new product, more allows Ganoderma lucidum have a sudden rise in social status.
Ganoderma lucidum is adapted at growing containing in the matrix such as grape glucose, sucrose, starch, cellulose, lignin.The agricultural byproducts such as general wood chip, cotton seed hulls and corncob carry out production cultivation as medium.Because region is different, by the impact of raw material factor, Ganoderma lucidum is produced has certain limitation, is difficult to extensively carry out.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, a kind of Ganoderma lucidum substituting stuff cultivation medium of with low cost, successful is provided.
The present invention is achieved by the following technical solution:
Ganoderma lucidum substituting stuff cultivation technique, comprises the steps:
1) wood chip processing in early stage: pulverize after the trunk of trees after felling and branch peeling, dry for subsequent use;
2) batching, spice, windrow fermentation: prepare burden according to the formula of medium, first wood chip, auxiliary material, gypsum, sugar mixing are formed siccative, then Ensure Liquid liquid fully mixes formation medium matrix thoroughly, after spice terminates, windrow ferments, nutrient solution is fully absorbed by medium matrix, can pack;
3) sterilizing: will install Bag Material bag and put into sterilizing installation, high pressure steam sterilization or normal-pressure sterilization, moves into cooling chamber by material bag after sterilizing terminates, treats that temperature is down to less than 30 DEG C, can inoculate;
4) inoculate: by Ganoderma lucidum strain inoculation in medium, then postvaccinal bacterium bag is put into culturing room and cultivate, treat that mycelia covers with bacterium bag, proceed to fertility room after surperficial mycelia kink variable color and carry out sesame period management;
5) sesame period management is gone out: control indoor temperature, humidity, CO
2concentration, intensity of illumination, pH value;
6) flower bud period management is dredged: in the young flower bud phase, manually remove small and weak sesame body, leave and take sturdy sesame body;
7) gather: gather in time when Ganoderma lucidum is ripe;
Step 1) described in medium, be made up of the raw material of following weight parts: wood chip 35-70 part, auxiliary material 25-60 part, gypsum 0.5-1.5 part, sugared 0.1-1 part, with nutrient solution control material-water ratio be 1:1.3-1.5;
Described nutrient solution is made up of following part by weight composition:
PH is adjusted to be 6.0 with phosphate buffer;
Described promoter adopts following methods preparation:
1. Feedstock treating: selfheal is clean, drying, pulverizing;
2. Steam explosion treatment: steam blasting tank put into by material step 1. obtained, burst pressure 3.5Mpa, the dwell time maintains 200s;
3. process is distilled: distiller put into by material step 2. obtained, bottom distiller, heating flame or pass into steam, when hot steam is filled in distiller, steam, by condenser pipe, is introduced in condenser, then by oil water separator, collects essential oil;
4. high-shear homogenizing machine process: essential oil step 3. obtained is by high-shear homogenizing machine process, and linear velocity 30m/s, time 5min, makes nano-emulsion, and obtains with filtering with microporous membrane is degerming.
Step 1 of the present invention) described in pulverizing, it is 0.5-1.0cm that preferred powder is broken to particle diameter; Described medium is made up of the raw material of following weight parts: wood chip 42 parts, auxiliary material 53 parts, 1 part, gypsum, sugar 1 part, with nutrient solution control material-water ratio be 1:1.3-1.5; Described wood chip preferably derives from maple and vertebra wood; Described auxiliary material is one or more compositions in cotton seed hulls, wheat bran, corn flour, when used as a mixture, can be arbitrary proportion.
Step 2) described in windrow fermentation, preferred control fermentation heap internal temperature is 65-70 DEG C, fermentation time is 1-2h, described pack, preferred 17X35X0.05 grade polypropylene plastics fruiting bag, feeds about 400 grams, charging should not be expired very much, material bag two tying, sack is noted cleaning, and prevents later stage living contaminants;
Step 3) described in high pressure steam sterilization, preferably 121 DEG C of sterilizing 1.5-2 hour, normal-pressure sterilization, preferably 100 DEG C of sterilizing 10-12 hour.
Step 4) described in inoculation, preferably according to the inoculum concentration of 1-3% (w/w), described culturing room require clean, clean, ventilate, between bacterium bag, temperature remains between 22-25 DEG C, relative air humidity remains on 55-60, cultivates, generally cultivate 30-40d under non-illuminated conditions.
Step 5) described in go out sesame period management, preferably control indoor temperature 24-28 DEG C, humidity 75-85%, CO
2concentration 0.5-0.9, adopt fixed light source, intensity of illumination 300-4001x, pH value is 5.3-6.7.
Step 6) described in thin flower bud period management, preferably leave and take sturdy sesame body 1-2.
Compared with prior art, tool of the present invention has the following advantages:
1, in prior art, the substituting stuff cultivation medium of preparation glossy ganoderma, control material-water ratio, generally adopt running water or pure water, the present invention adopts special nutrient solution to control material-water ratio, being of high nutritive value of the Ganoderma lucidum obtained, polyoses content significantly increases than prior art, and output increases by more than 20%.
2, the nutrient solution that the present invention is used, by adding trace metal ion and plant promoting agent, can stimulate the propagation of various kinds of cell.The plant material composition particularly used have passed through steam blasting, distillation, high-shear homogenizing machine process, obtains the plant oil components of nanometer emulsus, and in medium, the compatibility of other formulas is good.
Embodiment
With embodiment, the invention will be further described below, but the present invention is not limited to these embodiments.
Embodiment 1-3:
Ganoderma lucidum substituting stuff cultivation technique, comprises the steps:
1) wood chip processing in early stage: being crushed to particle diameter after the trunk of trees after felling and branch being removed the peel is 0.5-1.0cm, dries for subsequent use;
2) batching, spice, windrow fermentation: prepare burden according to the formula of medium, first wood chip, auxiliary material, gypsum, sugar mixing are formed siccative, then Ensure Liquid liquid fully mixes formation medium matrix thoroughly, after spice terminates, windrow ferments, and controlling fermentation heap internal temperature is 65-70 DEG C, and fermentation time is 1-2h, nutrient solution is fully absorbed by medium matrix, can pack;
3) sterilizing: will install Bag Material bag and put into sterilizing installation, high pressure steam sterilization or normal-pressure sterilization, moves into cooling chamber by material bag after sterilizing terminates, treats that temperature is down to less than 30 DEG C, can inoculate;
4) inoculate: Ganoderma lucidum bacterial classification is inoculated in medium according to the inoculum concentration of 1-3% (w/w), then postvaccinal bacterium bag is put into culturing room to cultivate, culturing room requires clean, clean, ventilation, between bacterium bag, temperature remains between 22-25 DEG C, relative air humidity remains on 55-60, cultivates, generally cultivate 30-40d under non-illuminated conditions, treat that mycelia covers with bacterium bag, proceed to fertility room after surperficial mycelia kink variable color and carry out sesame period management;
5) sesame period management is gone out: control indoor temperature 24-28 DEG C, humidity 75-85%, CO
2concentration 0.5-0.9, adopt fixed light source, intensity of illumination 300-4001x, pH value is 5.3-6.7;
6) dredge flower bud period management: in the young flower bud phase, manually remove small and weak sesame body, leave and take sturdy sesame body 1-2;
7) gather: gather in time when Ganoderma lucidum is ripe;
Step 1) described in medium, be made up of the raw material of following weight parts: wood chip 35-70 part, auxiliary material 25-60 part, gypsum 0.5-1.5 part, sugared 0.1-1 part, with nutrient solution control material-water ratio be 1:1.3-1.5;
Described nutrient solution is made up of following part by weight composition:
PH is adjusted to be 6.0 with phosphate buffer;
Described promoter adopts following methods preparation:
1. Feedstock treating: selfheal is clean, drying, pulverizing;
2. Steam explosion treatment: steam blasting tank put into by material step 1. obtained, burst pressure 3.5Mpa, the dwell time maintains 200s;
3. process is distilled: distiller put into by material step 2. obtained, bottom distiller, heating flame or pass into steam, when hot steam is filled in distiller, steam, by condenser pipe, is introduced in condenser, then by oil water separator, collects essential oil;
4. high-shear homogenizing machine process: essential oil step 3. obtained is by high-shear homogenizing machine process, and linear velocity 30m/s, time 5min, makes nano-emulsion, and obtains with filtering with microporous membrane is degerming.
The cultivation method reference example of comparative example.
The constituent analysis of the Ganoderma lucidum adopting embodiment and comparative example to obtain:
Result shows, the Ganoderma lucidum nutrient component that the embodiment of the present invention obtains is significantly higher than comparative example.
Claims (4)
1. Ganoderma lucidum substituting stuff cultivation technique, is characterized in that, comprises the steps:
1) wood chip processing in early stage: pulverize after the trunk of trees after felling and branch peeling, dry for subsequent use;
2) batching, spice, windrow fermentation: prepare burden according to the formula of medium, first wood chip, auxiliary material, gypsum, sugar mixing are formed siccative, then Ensure Liquid liquid fully mixes formation medium matrix thoroughly, after spice terminates, windrow ferments, nutrient solution is fully absorbed by medium matrix, can pack;
3) sterilizing: will install Bag Material bag and put into sterilizing installation, high pressure steam sterilization or normal-pressure sterilization, moves into cooling chamber by material bag after sterilizing terminates, treats that temperature is down to less than 30 DEG C, can inoculate;
4) inoculate: by Ganoderma lucidum strain inoculation in medium, then postvaccinal bacterium bag is put into culturing room and cultivate, treat that mycelia covers with bacterium bag, proceed to fertility room after surperficial mycelia kink variable color and carry out sesame period management;
5) sesame period management is gone out: control indoor temperature, humidity, CO
2concentration, intensity of illumination, pH value;
6) flower bud period management is dredged: in the young flower bud phase, manually remove small and weak sesame body, leave and take sturdy sesame body;
7) gather: gather in time when Ganoderma lucidum is ripe;
Step 1) described in medium, be made up of the raw material of following weight parts: wood chip 35-70 part, auxiliary material 25-60 part, gypsum 0.5-1.5 part, sugared 0.1-1 part, with nutrient solution control material-water ratio be 1:1.3-1.5;
Described nutrient solution is made up of following part by weight composition:
PH is adjusted to be 6.0 with phosphate buffer;
Described promoter adopts following methods preparation:
1. Feedstock treating: selfheal is clean, drying, pulverizing;
2. Steam explosion treatment: steam blasting tank put into by material step 1. obtained, burst pressure 3.5Mpa, the dwell time maintains 200s;
3. process is distilled: distiller put into by material step 2. obtained, bottom distiller, heating flame or pass into steam, when hot steam is filled in distiller, steam, by condenser pipe, is introduced in condenser, then by oil water separator, collects essential oil;
4. high-shear homogenizing machine process: essential oil step 3. obtained is by high-shear homogenizing machine process, and linear velocity 30m/s, time 5min, makes nano-emulsion, and obtains with filtering with microporous membrane is degerming.
2. Ganoderma lucidum substituting stuff cultivation technique according to claim 1, is characterized in that: step 1) described in medium be made up of the raw material of following weight parts: wood chip 42 parts, auxiliary material 53 parts, 1 part, gypsum, sugar 1 part, with nutrient solution control material-water ratio be 1:1.3-1.5.
3. Ganoderma lucidum substituting stuff cultivation technique according to claim 1, is characterized in that: step 1) described in wood chip derive from maple and vertebra wood.
4. Ganoderma lucidum substituting stuff cultivation technique according to claim 1, is characterized in that: step 5) described in go out sesame period management, control indoor temperature 24-28 DEG C, humidity 75-85%, CO
2concentration 0.5-0.9, adopt fixed light source, intensity of illumination 300-4001x, pH value is 5.3-6.7.
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| CN201510982320.6A CN105359840A (en) | 2015-12-24 | 2015-12-24 | Substitute cultivation technology for Ganoderma lucidum Antler |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106818210A (en) * | 2017-02-06 | 2017-06-13 | 深圳市达利时实业有限公司 | A kind of bottle for Ganoderma lucidum plants culture medium and the bottle of Ganoderma lucidum plants cultural method |
| CN107567959A (en) * | 2017-09-29 | 2018-01-12 | 福建绿宝食品集团有限公司 | A kind of production method of Ganoderma lucidum |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106818210A (en) * | 2017-02-06 | 2017-06-13 | 深圳市达利时实业有限公司 | A kind of bottle for Ganoderma lucidum plants culture medium and the bottle of Ganoderma lucidum plants cultural method |
| CN107567959A (en) * | 2017-09-29 | 2018-01-12 | 福建绿宝食品集团有限公司 | A kind of production method of Ganoderma lucidum |
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