CN107164240A - A kind of good lucidum strain rejuvenation method of whiteness - Google Patents
A kind of good lucidum strain rejuvenation method of whiteness Download PDFInfo
- Publication number
- CN107164240A CN107164240A CN201710470403.6A CN201710470403A CN107164240A CN 107164240 A CN107164240 A CN 107164240A CN 201710470403 A CN201710470403 A CN 201710470403A CN 107164240 A CN107164240 A CN 107164240A
- Authority
- CN
- China
- Prior art keywords
- parts
- product
- culture medium
- culture
- whiteness
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
Prepare and cultivate the invention discloses a kind of good lucidum strain rejuvenation method of whiteness, including tissue disintegration, lucid ganoderma stock culture.The inventive method carries out rejuvenation, and mycelial growth state is good, and obtained mycelia whiteness, tight ness rating and growing way is good.
Description
Technical field
The present invention relates to a kind of lucidum strain rejuvenation method, the good lucidum strain rejuvenation method of particularly a kind of whiteness.
Background technology
Ganoderma lucidum is the drying fructification of On Polyporaceae red sesame or purple sesame.Ganoderma lucidum is a kind of precious medicinal fungi, category
In Basidiomycetes Polyporaceae Ganoderma, there is the medicinal history of more than 2,000 years in China.Ganoderma lucidum first recorded in《Sheng Nong's herbal classic》, the successive dynasties
Doctor thinks that ganoderma lucidum can treat a variety of diseases, is the precious medicine that strengthening by means of tonics is strengthened the body resistance to consolidate the constitution.GL-B be in ganoderma lucidum most
One of effective composition, is present in the fructification of ganoderma lucidum, conidia powder and mycelium, with suppression tumour, enhancing body to certainly
The ability removed by base, thus can reduce free radical to the damage of body, have the effect of anti-aging, can also improve immunity,
Anti-inflammatory, reducing blood lipid, hypoglycemic etc. are acted on.
As the health-care efficacy of ganoderma lucidum is widely recognized and received by people, Wild ganoderma far can not meet people's
Demand.Culture medium is ganoderma lucidum conditions on which persons or things depend for existence, different culture raw materials and with can to the mycelial growth of ganoderma lucidum, yield and
Active component etc. produces important influence.Wherein, quality influence of the rejuvenation culture medium on the finished product ganoderma lucidum in later stage is very big.It is existing
Most of culture medium used comprises only glucose, potato, peptone etc. in rejuvenation method.The culture medium nutrition being so made is not
It is sufficient so that out of order, obtained mycelia whiteness, tight ness rating and growing way is bad for mycelial growth during rejuvenation.
The content of the invention
The purpose of the present invention, is to provide a kind of good lucidum strain rejuvenation method of whiteness.The inventive method carries out rejuvenation,
Mycelial growth state is good, and obtained mycelia whiteness, tight ness rating and growing way is good.
What the present invention was realized in.
A kind of good lucidum strain rejuvenation method of whiteness, including tissue disintegration, lucid ganoderma stock culture are prepared and cultivated.
The good lucidum strain rejuvenation method of foregoing whiteness, specifically includes following steps：
(1) tissue disintegration：Take the fructification of Wild ganoderma, rinsed well with water, cut stem, then sterilize, clean after inhale
Solid carbon dioxide point, cap cuts in half, and is cut into 1-3mm meat bacteria organization's piece in cap and stem connection centre, is placed in sterile culture
In ware, then tissue is inoculated on test tube slant culture medium, cultivates, cultivate 5-7d under the conditions of 26 DEG C~28 DEG C, obtain tissue
Mycelia after division；
(2) prepared by lucid ganoderma stock culture：Test tube is with after 70-80% alcohol disinfecting, and wiped clean is placed in 1,000,000 grades of ultra-clean works
Make platform, use ultraviolet lamp disinfection 25-35min, load culture medium, the charge weight of culture medium is the 2/3 of test tube volume, takes tissue disintegration
The mycelia fritter of mycelial growth afterwards foremost in 1-2cm, is put into culture medium, and mycelia fritter is covered with culture medium, and covering is thick
Spend for 1-1.5cm, fill in test tube plug, be put into 25-28 DEG C of insulating box and carry out culture 5-7 days, obtain lucid ganoderma stock culture；
(3) preparation of lucidum strain：Lucid ganoderma stock culture continues to cultivate 10-15 days, obtains lucidum strain；
(4) cultivate：Corn flour is taken, adds water and mixes the wet water content for causing corn flour for 50-60% maize powder medium, will
Lucidum strain is placed in the test tube equipped with millet powder culture medium, fills in test tube plug, in 25-28 DEG C of culture, treats that mycelial growth is completely tried
Stop culture during pipe, you can.
The good lucidum strain rejuvenation method of foregoing whiteness, the culture medium is calculated by weight, mainly by glucose
15-25 parts, 3-5 parts of yeast extract, 180-220 parts of potato, 1.5-2.5 parts of peptone, 15-25 parts of seawood meal, garlic powder 0.3-0.5
5-15 parts of part, 10-20 parts of bitter buckwheat, 1-10 parts of the coptis, 1-10 parts of the achene of Siberian cocklebur and the bighead atractylodes rhizome are made.
The good lucidum strain rejuvenation method of foregoing whiteness, the culture medium is calculated by weight, mainly by glucose
18-22 parts, 3-5 parts of yeast extract, 190-210 parts of potato, 1.8-2.2 parts of peptone, 18-22 parts of seawood meal, garlic powder 0.3-0.5
8-12 parts of part, 13-17 parts of bitter buckwheat, 3-7 parts of the coptis, 3-7 parts of the achene of Siberian cocklebur and the bighead atractylodes rhizome are made.
The good lucidum strain rejuvenation method of foregoing whiteness, the culture medium is calculated by weight, mainly by glucose
20 parts, 4 parts of yeast extract, 200 parts of potato, 2.0 parts of peptone, 20 parts of seawood meal, 0.4 part of garlic powder, 15 parts of bitter buckwheat, 5 parts of the coptis,
10 parts of 5 parts of the achene of Siberian cocklebur and the bighead atractylodes rhizome are made.
The good lucidum strain rejuvenation method of foregoing whiteness, the culture medium is prepared according to the following steps：
(1) bitter buckwheat, the coptis, the achene of Siberian cocklebur and the bighead atractylodes rhizome are taken, plus 6-8 times is measured water, decocts 2-3h, filtering is taken after filter residue drying, powder
Fine grained is broken into, A product are obtained；
(2) potato slice, plus 5-7 times measured water and boiled 20-30 minutes, is filtered, filter residue adds 5-7 times to measure water continuation, is heated to
50-60 DEG C, obtain B product；
(3) glucose, peptone, yeast extract, garlic powder, A product and seawood meal are sequentially added in B product, it is stirring while adding, stir
After even, C product are obtained；
(4) C product are dispensed to the 1/5 of 180ml test tubes, and silica gel plug sealing, 6-8 draws newspaper and wrapped, sterilized at 120-122 DEG C
After 20-30min, 50-60 DEG C is cooled to, 5-15 degree is tilted and is positioned in 20-35 DEG C of baking oven, after placing 24-48 hours, take
Go out, produce.
The good lucidum strain rejuvenation method of foregoing whiteness, the culture medium is prepared according to the following steps：
(1) bitter buckwheat, the coptis, the achene of Siberian cocklebur and the bighead atractylodes rhizome, plus 7 times of amount water are taken, 2.5h is decocted, filtering takes after filter residue drying, crushed
Into fine grained, A product are obtained；
(2) potato slice, plus 6 times of amount water boil 25 minutes, filter, and filter residue adds 6 times of amount water to continue, and is heated to 50-60 DEG C,
Obtain B product；
(3) glucose, peptone, yeast extract, garlic powder, A product and seawood meal are sequentially added in B product, it is stirring while adding, stir
After even, C product are obtained；
(4) C product are dispensed to the 1/5 of 180ml test tubes, silica gel plug sealing, and 7, which draw newspaper, wraps, and is sterilized at 120-122 DEG C
After 25min, 50-60 DEG C is cooled to, 10 degree is tilted and is positioned in 20-35 DEG C of baking oven, after placing 36 hours, takes out, produces.
Applicant has carried out substantial amounts of research to ganoderma lucidum rejuvenation method, and part Experiment is as follows：
Experimental example mycelial growths situation is investigated
1 material
1.1 ganoderma lucidum：There is provided by edible mushroom research institute of Shouguang City of Shandong Province.
1.2 contrast culture mediums：
Raw material：Glucose 20kg, yeast extract 4kg, potato 200kg, peptone 2.0kg, seawood meal 20kg and garlic powder
0.4kg。
Manufacture craft：
(1) potato slice, plus 6 times of amount water boil 25 minutes, filter, and filter residue adds 6 times of amount water to continue, and is heated to 50-60 DEG C,
Obtain A product；
(2) glucose, peptone, yeast extract, garlic powder and seawood meal are sequentially added in A product, it is stirring while adding, stir evenly
Afterwards, B product are obtained；
(3) B product are dispensed to the 1/5 of 180ml test tubes, silica gel plug sealing, and 7, which draw newspaper, wraps, and is sterilized at 120-122 DEG C
After 25min, 50-60 DEG C is cooled to, 10 degree is tilted and is positioned in 20-35 DEG C of baking oven, after placing 36 hours, takes out, produces.
2 test methods and result
Ganoderma lucidum is divided into 2 groups, of the present invention group and control group, present invention group carries out rejuvenation by embodiment 1.Control group rejuvenation side
Method be the same as Example 1, but when preparation and the second incubation of lucidum strain, culture medium used is above-mentioned contrast culture medium.During
The growing state of mycelia is observed, and is noted down.It the results are shown in Table 1 and table 2.
Mycelia climbs wall situation in the different rejuvenation culture mediums of table 1
The growth form of table 2 observes result
| Group | Mycelia whiteness | Tight ness rating | Mycelium growth vigor |
| Of the present invention group | It is pure white | Closely | It is very vigorous |
| Control group | Dark brightness | Closely | It is vigorous |
As seen from table, the Ganoderma lucidum mycelium growing way that the inventive method is carried out after rejuvenation is good, and growth form is carried out better than control group
The mycelia that rejuvenation is obtained.
Compared with prior art, rejuvenation is carried out with the inventive method, mycelial growth state is good, obtained mycelia whiteness,
Tight ness rating and growing way are good.
Embodiment
Embodiment 1.
Culture medium raw material：Glucose 20kg, yeast extract 4kg, potato 200kg, peptone 2.0kg, seawood meal 20kg, garlic
Powder 0.4kg, bitter buckwheat 15kg, coptis 5kg, achene of Siberian cocklebur 5kg and bighead atractylodes rhizome 10kg.
The preparation method of culture medium：Specifically include following steps：
(1) bitter buckwheat, the coptis, the achene of Siberian cocklebur and the bighead atractylodes rhizome, plus 7 times of amount water are taken, 2.5h is decocted, filtering takes after filter residue drying, crushed
Into fine grained, A product are obtained；
(2) potato slice, plus 6 times of amount water boil 25 minutes, filter, and filter residue adds 6 times of amount water to continue, and is heated to 50-60 DEG C,
Obtain B product；
(3) glucose, peptone, yeast extract, garlic powder, A product and seawood meal are sequentially added in B product, it is stirring while adding, stir
After even, C product are obtained；
(4) C product are dispensed to the 1/5 of 180ml test tubes, silica gel plug sealing, and 7, which draw newspaper, wraps, and is sterilized at 120-122 DEG C
After 25min, 50-60 DEG C is cooled to, 10 degree is tilted and is positioned in 20-35 DEG C of baking oven, after placing 36 hours, takes out, produces.
Rejuvenation of spawn method：Specifically include following steps：
(1) tissue disintegration：Take the fructification of Wild ganoderma, rinsed well with water, cut stem, then sterilize, clean after inhale
Solid carbon dioxide point, cap cuts in half, and is cut into 1-3mm meat bacteria organization's piece in cap and stem connection centre, is placed in sterile culture
In ware, then tissue is inoculated on test tube slant culture medium, cultivates, 6d is cultivated under the conditions of 26 DEG C~28 DEG C, obtain tissue point
Mycelia after splitting；
(2) prepared by lucid ganoderma stock culture：Test tube is with after 75% alcohol disinfecting, and wiped clean is placed in 1,000,000 grades of ultra-clean work
Platform, uses ultraviolet lamp disinfection 30min, loads culture medium, and the charge weight of culture medium is the 2/3 of test tube volume, is taken after tissue disintegration
The mycelia fritter of mycelial growth foremost in 1-2cm, is put into culture medium, and mycelia fritter is covered with culture medium, and cladding thickness is
1-1.5cm, fills in test tube plug, is put into 25-28 DEG C of insulating box and carries out culture 6 days, obtains lucid ganoderma stock culture；
(3) preparation of lucidum strain：Lucid ganoderma stock culture continues to cultivate 13 days, obtains lucidum strain；
(4) cultivate：Corn flour is taken, adds water and mixes wet so that the water content of corn flour is 55% maize powder medium, by spirit
Sesame strain is placed in the test tube equipped with millet powder culture medium, fills in test tube plug, in 25-28 DEG C of culture, treats the full test tube of mycelial growth
When stop culture, you can.
Embodiment 2.
Culture medium raw material：Glucose 25kg, yeast extract 5kg, potato 220kg, peptone 2.5kg, seawood meal 25kg, garlic
Powder 0.5kg, bitter buckwheat 20kg, coptis 10kg, achene of Siberian cocklebur 10kg and bighead atractylodes rhizome 15kg.
Culture medium preparation method：
(1) bitter buckwheat, the coptis, the achene of Siberian cocklebur and the bighead atractylodes rhizome, plus 8 times of amount water are taken, 3h is decocted, filtering takes after filter residue drying, is ground into
Fine grained, obtains A product；
(2) potato slice, plus 7 times of amount water boil 30 minutes, filter, and filter residue adds 7 times of amount water to continue, and is heated to 50-60 DEG C,
Obtain B product；
(3) glucose, peptone, yeast extract, garlic powder, A product and seawood meal are sequentially added in B product, it is stirring while adding, stir
After even, C product are obtained；
(4) C product are dispensed to the 1/5 of 180ml test tubes, silica gel plug sealing, and 8, which draw newspaper, wraps, and is sterilized at 120-122 DEG C
After 30min, 50-60 DEG C is cooled to, 15 degree is tilted and is positioned in 20-35 DEG C of baking oven, after placing 48 hours, takes out, produces.
Rejuvenation of spawn method：Specifically include following steps：
(1) tissue disintegration：Take the fructification of Wild ganoderma, rinsed well with water, cut stem, then sterilize, clean after inhale
Solid carbon dioxide point, cap cuts in half, and is cut into 1-3mm meat bacteria organization's piece in cap and stem connection centre, is placed in sterile culture
In ware, then tissue is inoculated on test tube slant culture medium, cultivates, 7d is cultivated under the conditions of 26 DEG C~28 DEG C, obtain tissue point
Mycelia after splitting；
(2) prepared by lucid ganoderma stock culture：Test tube is with after 80% alcohol disinfecting, and wiped clean is placed in 1,000,000 grades of ultra-clean work
Platform, uses ultraviolet lamp disinfection 35min, loads culture medium, and the charge weight of culture medium is the 2/3 of test tube volume, is taken after tissue disintegration
The mycelia fritter of mycelial growth foremost in 1-2cm, is put into culture medium, and mycelia fritter is covered with culture medium, and cladding thickness is
1-1.5cm, fills in test tube plug, is put into 25-28 DEG C of insulating box and carries out culture 7 days, obtains lucid ganoderma stock culture；
(3) preparation of lucidum strain：Lucid ganoderma stock culture continues to cultivate 15 days, obtains lucidum strain；
(4) cultivate：Corn flour is taken, adds water and mixes wet so that the water content of corn flour is 60% maize powder medium, by spirit
Sesame strain is placed in the test tube equipped with millet powder culture medium, fills in test tube plug, in 25-28 DEG C of culture, treats the full test tube of mycelial growth
When stop culture, you can.
Embodiment 3.
Culture medium raw material：Glucose 15kg, yeast extract 3kg, potato 180kg, peptone 1.5kg, seawood meal 15kg, garlic
Powder 0.3kg, bitter buckwheat 10kg, coptis 1kg, achene of Siberian cocklebur 1kg and bighead atractylodes rhizome 5kg.
Culture medium preparation method：
(1) bitter buckwheat, the coptis, the achene of Siberian cocklebur and the bighead atractylodes rhizome, plus 6 times of amount water are taken, 2h is decocted, filtering takes after filter residue drying, is ground into
Fine grained, obtains A product；
(2) potato slice, plus 5 times of amount water boil 20 minutes, filter, and filter residue adds 5 times of amount water to continue, and is heated to 50-60 DEG C,
Obtain B product；
(3) glucose, peptone, yeast extract, garlic powder, A product and seawood meal are sequentially added in B product, it is stirring while adding, stir
After even, C product are obtained；
(4) C product are dispensed to the 1/5 of 180ml test tubes, and silica gel plug sealing, 6-8 draws newspaper and wrapped, sterilized at 120-122 DEG C
After 20min, 50-60 DEG C is cooled to, 5 degree is tilted and is positioned in 20-35 DEG C of baking oven, after placing 24 hours, takes out, produces.
Rejuvenation of spawn method：Specifically include following steps：
(1) tissue disintegration：Take the fructification of Wild ganoderma, rinsed well with water, cut stem, then sterilize, clean after inhale
Solid carbon dioxide point, cap cuts in half, and is cut into 1-3mm meat bacteria organization's piece in cap and stem connection centre, is placed in sterile culture
In ware, then tissue is inoculated on test tube slant culture medium, cultivates, cultivate 5d under the conditions of 26 DEG C -28 DEG C, obtain tissue disintegration
Mycelia afterwards；
(2) prepared by lucid ganoderma stock culture：Test tube is with after 70% alcohol disinfecting, and wiped clean is placed in 1,000,000 grades of ultra-clean work
Platform, uses ultraviolet lamp disinfection 25min, loads culture medium, and the charge weight of culture medium is the 2/3 of test tube volume, is taken after tissue disintegration
The mycelia fritter of mycelial growth foremost in 1-2cm, is put into culture medium, and mycelia fritter is covered with culture medium, and cladding thickness is
1-1.5cm, fills in test tube plug, is put into 25-28 DEG C of insulating box and carries out culture 5 days, obtains lucid ganoderma stock culture；
(3) preparation of lucidum strain：Lucid ganoderma stock culture continues to cultivate 10 days, obtains lucidum strain；
(4) cultivate：Corn flour is taken, adds water and mixes wet so that the water content of corn flour is 50% maize powder medium, by spirit
Sesame strain is placed in the test tube equipped with millet powder culture medium, fills in test tube plug, in 25-28 DEG C of culture, treats the full test tube of mycelial growth
When stop culture, you can.
Claims (7)
1. a kind of good lucidum strain rejuvenation method of whiteness, it is characterised in that：Prepare and train including tissue disintegration, lucid ganoderma stock culture
Support.
2. the good lucidum strain rejuvenation method of whiteness according to claim 1, it is characterised in that：Specifically include following step
Suddenly：
（1）Tissue disintegration：Take the fructification of Wild ganoderma, rinsed well with water, cut stem, then sterilize, clean after blot water
Point, cap cuts in half, and is cut into 1-3mm meat bacteria organization's piece in cap and stem connection centre, is placed in sterile petri dish,
Tissue is inoculated on test tube slant culture medium again, is cultivated, is cultivated 5-7d under the conditions of 26 DEG C~28 DEG C, obtain after tissue disintegration
Mycelia；
（2）It is prepared by lucid ganoderma stock culture：Test tube is with after 70-80% alcohol disinfecting, and wiped clean is placed in 1,000,000 grades of superclean benches,
Ultraviolet lamp disinfection 25-35min is used, loads culture medium, the charge weight of culture medium is the 2/3 of test tube volume, is taken after tissue disintegration
The mycelia fritter of mycelial growth foremost in 1-2cm, is put into culture medium, and mycelia fritter is covered with culture medium, and cladding thickness is
1-1.5cm, fills in test tube plug, is put into 25-28 DEG C of insulating box and carries out culture 5-7 days, obtains lucid ganoderma stock culture；
（3）The preparation of lucidum strain：Lucid ganoderma stock culture continues to cultivate 10-15 days, obtains lucidum strain；
（4）Culture：Corn flour is taken, adds water and mixes the wet water content for causing corn flour for 50-60% maize powder medium, by ganoderma lucidum
Strain is placed in the test tube equipped with millet powder culture medium, fills in test tube plug, in 25-28 DEG C of culture, when mycelial growth full test tube
Stop culture, you can.
3. the good lucidum strain rejuvenation method of whiteness as claimed in claim 2, it is characterised in that：The culture medium is by weight
Part calculates, mainly by 15-25 parts of glucose, 3-5 parts of yeast extract, 180-220 parts of potato, 1.5-2.5 parts of peptone, seawood meal
5-15 parts of 15-25 parts, 0.3-0.5 parts of garlic powder, 10-20 parts of bitter buckwheat, 1-10 parts of the coptis, 1-10 parts of the achene of Siberian cocklebur and the bighead atractylodes rhizome are made.
4. the good lucidum strain rejuvenation method of whiteness as claimed in claim 3, it is characterised in that：The culture medium is by weight
Part calculates, mainly by 18-22 parts of glucose, 3-5 parts of yeast extract, 190-210 parts of potato, 1.8-2.2 parts of peptone, seawood meal
8-12 parts of 18-22 parts, 0.3-0.5 parts of garlic powder, 13-17 parts of bitter buckwheat, 3-7 parts of the coptis, 3-7 parts of the achene of Siberian cocklebur and the bighead atractylodes rhizome are made.
5. the good lucidum strain rejuvenation method of whiteness as claimed in claim 4, it is characterised in that：The culture medium is by weight
Part calculates, mainly by 20 parts of glucose, 4 parts of yeast extract, 200 parts of potato, 2.0 parts of peptone, 20 parts of seawood meal, garlic powder 0.4
10 parts of part, 15 parts of bitter buckwheat, 5 parts of the coptis, 5 parts of the achene of Siberian cocklebur and the bighead atractylodes rhizome are made.
6. the good lucidum strain rejuvenation method of whiteness as any one of claim 4-5, it is characterised in that：The training
Foster base is prepared according to the following steps：
（1）Bitter buckwheat, the coptis, the achene of Siberian cocklebur and the bighead atractylodes rhizome are taken, plus 6-8 times is measured water, decocts 2-3h, filtering takes after filter residue drying, is ground into
Fine grained, obtains A product；
（2）Potato slice, plus 5-7 times measured water and boiled 20-30 minutes, is filtered, filter residue adds 5-7 times to measure water continuation, is heated to 50-60
DEG C, obtain B product；
（3）Glucose, peptone, yeast extract, garlic powder, A product and seawood meal are sequentially added in B product, it is stirring while adding, stir evenly
Afterwards, C product are obtained；
（4）C product are dispensed to the 1/5 of 180ml test tubes, and silica gel plug sealing, 6-8 draws newspaper and wrapped, and sterilize 20- at 120-122 DEG C
After 30min, 50-60 DEG C is cooled to, 5-15 degree is tilted and is positioned in 20-35 DEG C of baking oven, after placing 24-48 hours, is taken out, i.e.,
.
7. the good lucidum strain rejuvenation method of whiteness as claimed in claim 6, it is characterised in that：The culture medium is by following
It is prepared by step：
（1）Bitter buckwheat, the coptis, the achene of Siberian cocklebur and the bighead atractylodes rhizome, plus 7 times of amount water are taken, 2.5h is decocted, filtering takes after filter residue drying, is ground into thin
Particle, obtains A product；
（2）Potato slice, plus 6 times of amount water boil 25 minutes, filter, and filter residue adds 6 times of amount water to continue, and is heated to 50-60 DEG C, obtains B
Product；
（3）Glucose, peptone, yeast extract, garlic powder, A product and seawood meal are sequentially added in B product, it is stirring while adding, stir evenly
Afterwards, C product are obtained；
（4）C product are dispensed to the 1/5 of 180ml test tubes, silica gel plug sealing, and 7, which draw newspaper, wraps, and sterilize 25min at 120-122 DEG C
Afterwards, 50-60 DEG C is cooled to, 10 degree is tilted and is positioned in 20-35 DEG C of baking oven, after placing 36 hours, takes out, produces.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710470403.6A CN107164240A (en) | 2017-06-20 | 2017-06-20 | A kind of good lucidum strain rejuvenation method of whiteness |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710470403.6A CN107164240A (en) | 2017-06-20 | 2017-06-20 | A kind of good lucidum strain rejuvenation method of whiteness |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107164240A true CN107164240A (en) | 2017-09-15 |
Family
ID=59818960
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710470403.6A Pending CN107164240A (en) | 2017-06-20 | 2017-06-20 | A kind of good lucidum strain rejuvenation method of whiteness |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107164240A (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104956914A (en) * | 2015-06-18 | 2015-10-07 | 唐伟 | Breeding method for natural ganoderma lucidum |
-
2017
- 2017-06-20 CN CN201710470403.6A patent/CN107164240A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104956914A (en) * | 2015-06-18 | 2015-10-07 | 唐伟 | Breeding method for natural ganoderma lucidum |
Non-Patent Citations (4)
| Title |
|---|
| 丁安伟等: "《中药资源综合利用与产品开发》", 30 April 2013 * |
| 崔月花等: "几种中药对灵芝发酵影响的研究", 《食用菌学报》 * |
| 张真等: "《"浙八味"中药材规范化生产技术》", 31 December 2007 * |
| 王玉红等: "苦荞对灵芝发酵生产灵芝酸的影响", 《食品与发酵工业》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US10266695B2 (en) | Cultivation of Xylaria species biomass as a binding agent in material production | |
| WO2015180519A1 (en) | Method for cultivating high-cordyceps-polysaccharide cordyceps militaris | |
| CN109439499B (en) | Cordyceps militaris wine and preparation method thereof | |
| JP4908424B2 (en) | Brewing seed meal, brewing meal, brewed food, and production method thereof | |
| WO2011149130A1 (en) | Rice wine using turmeric and a production method for the same | |
| WO2019223288A1 (en) | Fermentation production process of pacilomyces hepiali chen cs-4 | |
| CN104911107A (en) | Aspergillus oryzae bacterial strain and application of aspergillus oryzae bacterial strain in soybean paste fermentation | |
| CN112155118A (en) | Preparation method of novel biological feed | |
| CN107164236A (en) | A kind of preparation method of lucid ganoderma stock culture | |
| CN105165409B (en) | The method that indoor industrially produces polyporus rhinoceros cooke mushroom | |
| CN114592013A (en) | Preparation method and application of cordyceps sobolifera enzyme | |
| CN106819861A (en) | Method for preventing tyrosine crystallization of soybean paste | |
| KR101174905B1 (en) | Apparatus and method for making alcohoic berverages with detoxified lacquer | |
| CN107164240A (en) | A kind of good lucidum strain rejuvenation method of whiteness | |
| KR101470950B1 (en) | Manufacturing Method for Distilled Liquor Using Pear and mulberry | |
| CN107245455A (en) | A kind of lucidum strain rejuvenation method | |
| KR101274638B1 (en) | Method or manufacturing improved nuruk for raw rice wine | |
| CN107267399A (en) | A kind of good lucidum strain rejuvenation method of mycelium growth vigor | |
| CN109965217B (en) | Bean cotyledon sweet petal and preparation method thereof | |
| KR20130113608A (en) | Koji for gochujang having high enzymatic activity, gochujang using the koji, and methods thereof | |
| CN106866235A (en) | A kind of Antrodia camphorata ware training culture medium and preparation method thereof | |
| CN113151005B (en) | Monascus purpureus W-4 capable of producing lovastatin at high yield and application thereof | |
| CN114431460A (en) | Pure natural edible fungus nutrition powder and preparation method thereof | |
| CN104762166B (en) | A kind of fed-batch cultivation method preparing yellow wine with high alcohol content | |
| CN110607332B (en) | Culture medium for improving content of functional red yeast rice Monacolin K and fermentation method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170915 |
|
| RJ01 | Rejection of invention patent application after publication |