CN107118950A - A kind of bacterium automatic vaccination instrument - Google Patents
A kind of bacterium automatic vaccination instrument Download PDFInfo
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- CN107118950A CN107118950A CN201710314288.3A CN201710314288A CN107118950A CN 107118950 A CN107118950 A CN 107118950A CN 201710314288 A CN201710314288 A CN 201710314288A CN 107118950 A CN107118950 A CN 107118950A
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- 241000894006 Bacteria Species 0.000 title claims abstract description 86
- 238000002255 vaccination Methods 0.000 title claims abstract description 25
- 238000011081 inoculation Methods 0.000 claims abstract description 110
- 238000012360 testing method Methods 0.000 claims abstract description 80
- 230000001954 sterilising effect Effects 0.000 claims abstract description 39
- 230000000844 anti-bacterial effect Effects 0.000 claims abstract description 37
- 230000001580 bacterial effect Effects 0.000 claims description 27
- 238000004659 sterilization and disinfection Methods 0.000 claims description 24
- 239000001963 growth medium Substances 0.000 claims description 14
- 239000011148 porous material Substances 0.000 claims description 13
- 238000002372 labelling Methods 0.000 claims description 10
- 238000001514 detection method Methods 0.000 claims description 7
- 230000035939 shock Effects 0.000 claims description 6
- 238000005070 sampling Methods 0.000 claims description 5
- 239000007921 spray Substances 0.000 claims description 4
- 230000001376 precipitating effect Effects 0.000 claims description 3
- 230000005540 biological transmission Effects 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- 230000005611 electricity Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 14
- 210000001503 joint Anatomy 0.000 abstract description 3
- 238000012009 microbiological test Methods 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 13
- 230000033001 locomotion Effects 0.000 description 10
- 238000013461 design Methods 0.000 description 9
- 230000008569 process Effects 0.000 description 9
- 230000009471 action Effects 0.000 description 7
- 230000007423 decrease Effects 0.000 description 7
- 238000000151 deposition Methods 0.000 description 5
- 239000007788 liquid Substances 0.000 description 4
- 239000012472 biological sample Substances 0.000 description 3
- 230000002906 microbiologic effect Effects 0.000 description 3
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 238000004904 shortening Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 206010048723 Multiple-drug resistance Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 238000012549 training Methods 0.000 description 1
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/14—Rotation or movement of the cells support, e.g. rotated hollow fibers
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- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
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- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
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Abstract
The present invention relates to Micro biological Tests instrument and equipment field, specifically say a kind of bacterium automatic vaccination instrument, including body, test tube storing unit, culture dish conveyer, classification inoculation apparatus, bactericidal unit, clamping device and take lid arrangement, described test tube storing unit is arranged on After-Body, described culture dish conveyer is arranged in front of test tube storing unit, described classification inoculation apparatus is arranged on test tube storing unit right, described bactericidal unit is arranged in the left and right sides of the scribing position of classification inoculation apparatus, described takes lid arrangement to be arranged on body, described clamping device is arranged on body.The instrumentation of the invention that mainly passes through replaces artificial operation, can be achieved a butt joint the automatic sterilizing of kind of environment and microbionation ring, shorten whole inoculation time simultaneously, and the operating efficiency of microbionation and line is improved, reach that accuracy when microbionation and line is high and is inoculated with Utopian effect.
Description
Technical field
The present invention relates to Micro biological Tests instrument and equipment field, a kind of bacterium automatic vaccination instrument is specifically said.
Background technology
Current micro-biological samples processing is mostly artificial operation inoculation, in the prior art, to individual's operation level requirement compared with
Height, does not merely have to accomplish sterile working, and to be put into practice by strict training and long period of operation, could obtain preferably inoculation effect
Really, secondly, Inoculation Method overall efficiency is low, and is influenceed by the personal level of operator and inoculation custom, part operator
There may be delay or situations such as slow movement, cause to dip bacterial exposure aerial time on oese it is longer and
Influence Bacteria Culture effect, this classical microbionation method inefficiency, it is difficult to carry out accuracy and implementation quality control
System, it is impossible to meet the laboratory of modernization to automation, the requirement of accuracy.
In consideration of it, using micro-biological samples handling machine people or the alternative artificial operation of micro-biological samples process instrumentation, it is real
Existing system for automatic marker making inoculation, i.e., a kind of to realize the bacterium automatic vaccination instrument of plane method of scoring, its specific effect is as follows：
1. a kind of bacterium automatic vaccination instrument of the present invention, it instead of during microbionation is manually inoculated with
Operation, and the automatic sterilizing of can achieve a butt joint kind of environment and microbionation ring, and take bacterium and take lid to carry out simultaneously, shorten
The time of whole seeded process, improve operating efficiency when microbionation and line.
2. a kind of bacterium automatic vaccination instrument of the present invention, the design of its shock module so that bacterium of the invention is molten
Liquid will not be precipitated in test tube, effectively ensure in test tube that bacterial solution is evenly distributed the effect precisely sampled with classification inoculation apparatus
Really.
3. a kind of bacterium automatic vaccination instrument of the present invention, the design of its infrared distance sensor and test point so that
Angles and positions accuracy of the present invention in microbionation, line, has reached that accuracy when microbionation, line is high and connects
Plant Utopian effect.
The content of the invention
In order to make up the deficiencies in the prior art, the invention provides a kind of bacterium automatic vaccination instrument, it can be trained in bacterium
Traditional artificial plate streaking is replaced with automated procedures during supporting, it is to avoid the operating efficiency of microbionation in the prior art
Position when low, Multiple drug resistance is uneven and is inoculated with, rules is inaccurate, realizes the Utopian function of effect of inoculation.
The technical solution adopted for the present invention to solve the technical problems is：A kind of bacterium automatic vaccination instrument, including body, examination
Pipe storing unit, culture dish conveyer, classification inoculation apparatus, bactericidal unit, clamping device and take lid arrangement；Described test tube storage
Device is arranged on After-Body and fills germy test tube for storage；Described culture dish conveyer is deposited installed in test tube
In front of device and for transporting culture dish；Described classification inoculation apparatus is arranged on test tube storing unit right, and classification inoculation apparatus is used to dip in
The bacterium in test tube storing unit is taken, and bacterium is transported to above culture dish conveyer, be i.e. scribing position, classification inoculation apparatus is used
In to culture dish progress plate streaking；Described bactericidal unit is arranged on body and directly over culture dish conveyer,
Bactericidal unit is used to play during microbionation to surrounding environment and to the sterilization functions of classification inoculation apparatus, and bactericidal unit includes
For microbionation ring is sterilized a sterilization unit, No. two sterilization units, positions positioned at a sterilization unit right
No. two test points in front of a sterilization unit and No. three test points directly over the culture dish that will be rule, No. one goes out
The flame spray head for sterilizing is mounted on bacterium unit and No. two sterilization units, during work, microbionation ring is in classification inoculation apparatus
Drive under, move at bactericidal unit, when No. two test points detect microbionation ring reach bactericidal unit at when, inoculation dress
Stop motion is put, subsequent flame spray head carries out disinfecting action to microbionation ring, miscellaneous bacteria on microbionation ring is eliminated to reach
Purpose；Described clamping device is arranged on body, and for clamping culture dish, described takes lid arrangement to be arranged on body simultaneously
For carrying out taking lid to culture dish, described clamping device is arranged on body, and for clamping culture dish.
The pore of depositing for depositing test tube is evenly arranged with described test tube storing unit, respectively deposits and rubber is equipped with pore
Rubber cushion, is in order to prevent the test tube caused by the reasons such as vibrations, activity from damaging, while rubber blanket is because with certain provided with rubber blanket
Elasticity, be suitable for the test tube of different-diameter, improve the scope of application, test tube is placed in the rubber blanket for depositing pore, in test tube
For depositing bacterial solution, and respectively deposit and be mounted at pore for detecting the test point whether classification inoculation apparatus arrives, one
Number test point purpose of design is to be accurately positioned in seeded process to classification inoculation apparatus；In the middle part of described test tube storing unit
Shock module is also equipped with, vibration module is used to produce micro-vibration, on the one hand can prevent bacterial solution from precipitating, on the other hand favourable
In the accurate sampling of classification inoculation apparatus.
Described culture dish conveyer includes culture dish, conveyer belt and labelling module, and described culture dish includes ware
Seat, ware lid and the culture medium being placed in ware seat, culture dish are uniformly placed on a moving belt；Described conveyer belt is arranged on body
Go up and be used for the underface that culture dish is transported to classification inoculation apparatus scribing position；Described labelling module is arranged on body simultaneously
For being labelled to culture medium, label is used for the essential information for recording the culture medium after inoculation, when such as bacterial species, inoculation
Between etc. information.During work, when needing to carry out plate streaking, then placed first by manually will be equipped with the culture dish of culture medium successively
On a moving belt, then conveyer belt drives culture dish to turn left Uniform Movement to being rule immediately ahead of classification inoculation apparatus from the right side, line
After finishing, the culture dish for being inoculated with completion is transported at labelling module is labelled again by conveyer belt.
Described classification inoculation apparatus includes inoculation rotating disk, inoculation bar, swing arm motor, is inoculated with transverse slat, takes bacterium electric pushrod, one
Number infrared ray range sensor, bacterium module and No. two infrared distance sensors are taken, described inoculation rotating disk is arranged on body,
It is inoculated with bar and is automatic telescopic link and is arranged on by shaft coupling in inoculation rotating disk that described swing arm motor is arranged on inoculation bar
End；Described inoculation transverse slat level is arranged on swing arm motor upper end by shaft coupling, and inoculation transverse slat front end is vertically-mounted to take bacterium
Electric pushrod, a described infrared ray range sensor is arranged on inoculation transverse slat, and an infrared sensor is used to detect one
Number test point, is easy to being accurately positioned for classification inoculation apparatus when classification inoculation apparatus is sampled；It is described to take bacterium module to be arranged on to take bacterium electronic
Push rod lower end, takes bacterium module to include fixed block, lifting module, microbionation ring and No. three infrared distances, described fixed block weldering
It is connected in and takes on bacterium electric pushrod, described microbionation ring includes three microbionation rings being sequentially arranged from left to right, is located at
The microbionation ring of the leftmost side is used to dip bacterial solution, and other two microbionation rings are used for plate streaking, described No. three
Infrared distance sensor is arranged on fixed block, and No. three infrared distance sensors are used for detection bacterium oese apart from bacterial solution
Height, it is to avoid microbionation ring is not in contact with to bacterial solution or to stretch into bacterial solution long；No. two described infrared sensings
Device is arranged on inoculation transverse slat, and No. two infrared distance sensors are used to detect No. two test points, are easy to when bactericidal unit sterilizes
Bactericidal unit is accurately positioned.During work, classification inoculation apparatus is located above bactericidal unit to sterilize to microbionation ring, has sterilized
Cheng Hou, bactericidal unit extinguishes, and inoculation rotating disk is rotated clockwise, and in rotation process, an infrared distance sensor is detected
A number test point, now microbionation ring be located at directly over test tube, be then inoculated with bar and decline and shorten oese and bacterial solution
Distance, then takes bacterium electric pushrod to decline and bacterial solution is dipped, then take bacterium electric pushrod to retract, and inoculation bar, which rises, to be made
Microbionation ring leaves test tube, is then inoculated with rotating disk and rotates counterclockwise, No. two infrared distance sensors detect No. three detections
Point, now microbionation ring is directly over culture dish, and inoculation rotating disk stops operating, and inoculation bar declines, while bactericidal unit
Light and surrounding environment is sterilized, under the cooperation of clamping device, the common first time line for completing microbionation ring, first
After the completion of secondary line, inoculation bar is risen, and swing arm motor is rotated clockwise, and No. two infrared distance sensors detect No. two detections
Point, now microbionation ring is directly over bactericidal unit, and two microbionation rings positioned at right stretch out thin with the leftmost side
Bacterium oese is consistent, and now bactericidal unit carries out sterilizing simultaneously to three microbionation rings, after the completion of sterilizing, swing arm motor
Rotate counterclockwise and return to scribing position by No. two infrared sensors and carry out second of line, three microbionation rings are in clamping
Rule simultaneously under the cooperation of device, then repeat above-mentioned action and carry out third time line, terminate repetitive operation.Connect in above-mentioned bacterium
During kind ring dips bacterial solution, the vibration module moment produces vibration, and the vibration that vibration module is produced can make bacterial solution
Surface produces ripple, and corrugated surface advantageously forms diffusing reflection, is conducive to the accurate detection of No. three infrared distance sensors.
Described clamping device be located at scribing position at and for culture dish clamping, clamping device include after folder unit and
Fore-clamp unit, described rear folder unit is installed on body with fore-clamp unit and positioned at culture dish conveyer front and rear sides；
Folder unit, which includes being provided with the middle part of grip slipper, two sliding blocks, spring and upper driven pulley, grip slipper, afterwards is available for sliding block reciprocating
Chute, each sliding block is slidably mounted in chute, and spring is connected between each sliding block and grip slipper, and each slip front passes through
A piece connecting rod is connected with driven pulley, and upper driven pulley is in contact with the ware seat of culture dish, the purpose of the extensional motion design of spring
It is in order that the spacing between two upper driven pulleys can adjust, to adapt to the diameter of culture dish；Described fore-clamp unit includes folder
Push rod, gripper jaw, lower driving wheel, lower driven pulley and clamping motor are held, described clamping push rod is arranged on body, clamps push rod
End is fixed with gripper jaw, and gripper jaw madial wall is provided with lower driving wheel and lower driven pulley for being touched with culture dish ware seated connection,
Described clamping motor is arranged on gripper jaw and connects lower driving wheel.During work, clamping push rod, which will stretch out a certain distance, to be trained
Foster ware pushes upper driven pulley to, and culture dish is clamped under the collective effect of lower driving wheel, lower driven pulley and upper driven pulley, in flat board
During line, microbionation ring contact culture medium is motionless, then gripper jaw driving lower driving wheel clockwise movement, now culture dish meeting
And then rotate, microbionation ring is rule, after line certain distance, lower driving wheel stops operating, and microbionation ring rises, under
Driving wheel rotate counterclockwise makes culture dish be returned to initial position, and clamping push rod is retracted a certain distance backward, to carry out second
The line of section and then the progress third time line that repeats the above steps, three sections of lines complete to rule for the first time after having drawn, then bacterium
Oese rises and reaches sterilization station and sterilized, while clamping push rod and stretching out makes culture dish be returned to initial position, then
Clamping device coordinates three microbionation rings to rule simultaneously to complete second of line, is sterilized after the completion of second of line,
Repeat second of action and carry out third time line, until line is completed.
Described takes lid arrangement to include taking lid rotating disk, taking lid bar, take lid motor, take cover plate and take lid terminal, described
Lid rotating disk is taken to be arranged on body, described takes lid bar installed in taking in lid rotating disk, and it is automatic telescopic rod member to take lid bar, is taken
Lid bar is automatic telescopic rod member, takes lid bar upper end to be provided with and takes lid motor, and described to take lid motor to be servomotor, described takes lid
Plate level is arranged on and taken on lid motor, takes cover plate lower end to be provided with and takes lid terminal；Described takes lid terminal to include two horizontal reverses
Take cover plate lower surface to being arranged in takes lid push rod, grip block, elastomeric pad and pressure sensor, and described grip block is connected in
Lid push rod front end is respectively taken, described elastomeric pad is arranged on the inside of grip block and for being in contact with ware lid, elastomeric pad and folder
Hold and pressure sensor be installed between plate, described elastomeric pad heatproof is high, the purpose of elastomeric pad design be in order to avoid by
In the temperature of ware lid it is too high and cause to pressure sensor cause damage.During work, take cover plate to be located at directly over culture dish, take lid
Plate, which declines, to be made ware lid be located at two to take between lid terminal, then take lid push rod retraction to clamp ware lid, then take lid bar to rise general
Ware lid is removed from ware seat, after ware lid reaches certain altitude, and taking lid motor to rotate counterclockwise certain angle makes ware lid be located at sterilizing
Ware lid is sterilized above device, inoculation line work just can be now carried out, after the completion of inoculation, take lid motor to turn clockwise
It is dynamic, ware lid is located at immediately below ware seat, then take lid bar to rise, be next time take lid to prepare.
The beneficial effects of the invention are as follows：
1. a kind of bacterium automatic vaccination instrument of the present invention, it instead of during microbionation is manually inoculated with
Operation, and the automatic sterilizing of can achieve a butt joint kind of environment and microbionation ring, and take bacterium and take lid to carry out simultaneously, shorten
The time of whole seeded process, operating efficiency when improving microbionation and line.
2. a kind of bacterium automatic vaccination instrument of the present invention, the design of its shock module so that bacterium of the invention is molten
Liquid will not be precipitated in test tube, effectively ensure in test tube that bacterial solution is evenly distributed the effect precisely sampled with classification inoculation apparatus
Really.
3. a kind of bacterium automatic vaccination instrument of the present invention, the design of its infrared distance sensor test point so that this
Angles and positions accuracy of the invention in microbionation, line, has reached that accuracy when microbionation, line is high and is inoculated with
Utopian effect.
Brief description of the drawings
The present invention is further described with embodiment below in conjunction with the accompanying drawings.
Fig. 1 is the schematic top plan view of the present invention；
Structural representation when Fig. 2 is present invention line；
Fig. 3 is the structural representation of classification inoculation apparatus of the present invention；
Fig. 4 is the structural representation that the present invention takes lid arrangement；
Fig. 5 is Fig. 4 part-structure schematic diagram；
In figure：Including body 1, test tube storing unit 2, culture dish conveyer 3, classification inoculation apparatus 4, bactericidal unit 5, clamping
Device 6, take lid arrangement 7, deposit pore 21, rubber blanket 22, test point 23, shock module 24, culture dish 31, conveyer belt 32,
Labelling module 33, ware seat 311, ware lid 312, culture medium 313, inoculation rotating disk 41, inoculation bar 42, swing arm motor 43, inoculation
Transverse slat 44, take bacterium electric pushrod 45, infrared distance sensor 46, take bacterium module 47, it is No. two infrared distance sensors 48, solid
Determine block 471, lifting module 472, microbionation ring 473, No. three infrared distance sensors 474, sterilization unit 51, No. two go out
Bacterium unit 52, No. two test points 53, No. three test points 54, rear folder unit 61, fore-clamp unit 62, grip slipper 611, sliding block 612, bullets
Spring 613, upper driven pulley 614, clamping push rod 621, gripper jaw 622, lower driving wheel 623, lower driven pulley 624, clamping motor 625,
Lid rotating disk 71 is taken, lid bar 72 is taken, takes lid motor 73, take cover plate 74, take lid terminal 75, take lid push rod 741, grip block 742, bullet
Property pad 743, pressure sensor 744.
Embodiment
In order that the technical means, the inventive features, the objects and the advantages of the present invention are easy to understand, tie below
Embodiment is closed, the present invention is expanded on further.
As shown in Figures 1 to 5, a kind of bacterium automatic vaccination instrument of the present invention, including body 1, test tube storing unit
2nd, culture dish conveyer 3, classification inoculation apparatus 4, bactericidal unit 5, clamping device 6 and lid arrangement 7 is taken；Described test tube storing unit
2 are arranged on the rear portion of body 1 and fill germy test tube for storage；Described culture dish conveyer 3 is deposited installed in test tube
The front of device 2 simultaneously is used to transport culture dish；Described classification inoculation apparatus 4 is arranged on the right of test tube storing unit 2, and classification inoculation apparatus 4 is used
In dipping the bacterium in test tube storing unit 2, and bacterium is transported to the top of culture dish conveyer 3, i.e. scribing position, inoculation
Device 4 is used to carry out plate streaking to culture dish；Described bactericidal unit 5 is arranged on body 1 and transmits dress positioned at culture dish
Put directly over 3, bactericidal unit 5 is used to play during microbionation to surrounding environment and to the sterilization functions of classification inoculation apparatus 4,
Bactericidal unit 5 is including a sterilization unit 51 for being sterilized to microbionation ring, positioned at a right side of sterilization unit 51
No. two sterilization units 52, No. two test points 53 positioned at the front of sterilization unit 51 and No. three detections positioned at scribing position
The flame spray head for sterilizing is mounted on point 54, a sterilization unit 51 and No. two sterilization units 52；Described clamping dress
6 are put to be arranged on body 1, and for clamping culture dish, described takes lid arrangement 7 to be arranged on body 1 and for culture dish
Progress takes lid, and described clamping device 6 is arranged on body 1, and for clamping culture dish.
As shown in Figure 1, Figure 2 and Figure 3, a kind of bacterium automatic vaccination instrument of the present invention, described test tube storing unit 2
On be evenly arranged with and deposit pore 21 for deposit test tube, respectively deposit and rubber blanket 22 be equipped with pore 21, be provided with rubber blanket 22
In order to prevent the test tube caused by the reasons such as vibrations, activity from damaging, while rubber blanket 22 is because with certain elasticity, being suitable for not
With the test tube of diameter, the scope of application is improved, test tube is placed in the rubber blanket 22 for depositing pore 21, be used to deposit bacterium in test tube
Solution, and respectively deposit a test point 23 is mounted at pore 21, a purpose of design of test point 23 is easy in seeded process
Middle classification inoculation apparatus 4 is accurately positioned；The described middle part of test tube storing unit 2 is also equipped with shock module 24, and vibration module 24 is used
In producing micro-vibration, it on the one hand can prevent bacterial solution from precipitating, on the other hand be conducive to the accurate sampling of classification inoculation apparatus 4.
As shown in Figure 1, Figure 2 and Figure 3, a kind of bacterium automatic vaccination instrument of the present invention, described culture dish transmission dress
Putting 3 includes culture dish 31, conveyer belt 32 and labelling module 33, and described culture dish 31 includes ware seat 311, ware lid 312 and put
The culture medium 313 in ware seat 311 is put, culture dish 31 is uniformly placed on conveyer belt 32；Described conveyer belt 32 is arranged on machine
On body 1 and for culture dish 31 to be transported to the underface of the scribing position of classification inoculation apparatus 4；Described labelling module 33 is arranged on
On body 1 and for being labelled to culture medium 313, label is used for the essential information for recording the culture medium 313 after inoculation, such as
The information such as bacterial species, inoculation time.During work, when needing to carry out plate streaking, then pass through artificial or plant equipment first
Culture dish 31 is placed on conveyer belt 32 successively, then conveyer belt 32 drives culture dish 31 to turn left Uniform Movement to inoculation from the right side
The front of device 4 is rule, and after line is finished, the culture dish 31 for being inoculated with completion is transported to labelling by conveyer belt 32 again
Labelled at module 33.
Such as Fig. 1 and shown in 2, a kind of bacterium automatic vaccination instrument of the present invention, described classification inoculation apparatus 4 includes inoculation
Rotating disk 41, inoculation bar 42, swing arm motor 43, inoculation transverse slat 44, take bacterium electric pushrod 45, an infrared ray range sensor
46th, bacterium module 47 and No. two infrared distance sensors 48 are taken, described inoculation rotating disk 41 is arranged on body 1, is inoculated with bar 42
In inoculation rotating disk 41, described swing arm motor 43 is arranged on inoculation bar 42 upper end；The described level of inoculation transverse slat 44
On swing arm motor 43, inoculation transverse slat 44 front end is vertically-mounted to take bacterium electric pushrod 45, a described infrared ray away from
It is arranged on from sensor 46 on inoculation transverse slat 44, an infrared sensor 46 is used to detect a test point 23, in classification inoculation apparatus 4
It is easy to being accurately positioned for classification inoculation apparatus 4 during sampling；Described takes bacterium module 47 installed in the lower end of bacterium electric pushrod 45 is taken, and takes bacterium mould
Block 47 includes fixed block 471, lifting module 472, microbionation ring 473 and No. three infrared distance 474, described fixed block 471
It is connected in and takes on bacterium electric pushrod 45, described microbionation ring 473 includes three microbionations being sequentially arranged from left to right
Ring 474, the microbionation ring positioned at the leftmost side is used to dip bacterial solution, and other two microbionation rings are used for plate streaking,
No. three described infrared distance sensors 474 are arranged on fixed block 471, and No. three infrared distance sensors 474 are used to detect thin
Height of the bacterium oese 473 apart from bacterial solution, it is to avoid microbionation ring 473 is not in contact with to bacterial solution or to stretch into bacterium molten
Liquid is long；No. two described infrared sensors 48 are arranged on inoculation transverse slat 44, and No. two infrared distance sensors 48 are used to detect
No. two test points 53 and No. three test points 54, being accurately positioned for classification inoculation apparatus 4 is easy to when bactericidal unit 5 sterilizes.During work, connect
Plant device 4 to sterilize to microbionation ring 473 positioned at the top of bactericidal unit 5, after the completion of sterilizing, bactericidal unit 5 extinguishes, inoculation
Rotating disk 41 is rotated clockwise, in rotation process, and an infrared distance sensor 46 detects a test point 23, now carefully
Bacterium oese 473 is located at directly over test tube, is then inoculated with bar 42 and declines the distance for shortening oese 473 and bacterial solution, then
Take bacterium electric pushrod 45 to decline to dip bacterial solution, then take bacterium electric pushrod 45 to retract, inoculation bar 42, which rises, to be made carefully
Bacterium oese 473 leaves test tube, is then inoculated with rotating disk 41 and rotates counterclockwise, No. two infrared distance sensors 48 detect No. three
Test point 54, now microbionation ring 473 is directly over culture dish, and inoculation rotating disk 41 stops operating, and inoculation bar 42 declines,
Bactericidal unit 5 is lighted simultaneously sterilizes to surrounding environment, common to complete microbionation ring 473 under the cooperation of clamping device 6
First time line, for the first time rule after the completion of, inoculation bar 42 rise, swing arm motor 43 is rotated clockwise, No. two infrared distances
Sensor 48 detects No. two test points 53, and now microbionation ring 473 is located at directly over bactericidal unit 5, and two positioned at right
The individual stretching of microbionation ring 473 and the microbionation ring 473 of the leftmost side are consistent, and now three bacteriums of bactericidal unit 5 pair connect
Plant ring 473 sterilize simultaneously, after the completion of sterilizing, swing arm motor 43 is rotated counterclockwise and returned to by No. two infrared sensors 48
Scribing position carries out second and rule, and three microbionation rings 473 are rule simultaneously under the cooperation of clamping device 6, are then repeated
Above-mentioned action carries out third time line, repeats above-mentioned action until line terminates.Bacterial solution is dipped in above-mentioned microbionation ring
During, the vibration module moment produces vibration, and the vibration that vibration module is produced can make bacterial solution surface produce ripple, ripple
Face advantageously forms diffusing reflection, is conducive to accurate detection of No. three infrared distance sensors 474 to bacterial solution.
As shown in Figure 1, Figure 2 and Figure 4, a kind of bacterium automatic vaccination instrument of the present invention, described clamping device 6 is located at
At scribing position and for the clamping of culture dish 31, folder unit 61 and fore-clamp unit 62, described rear folder after clamping device 6 includes
Unit 61 is installed on body 1 with fore-clamp unit 62 and positioned at the front and rear sides of culture dish conveyer 3；Folder unit 61 includes afterwards
Grip slipper 611, two sliding blocks 612, spring 613 and upper driven pulley 614, the middle part of grip slipper 611, which is provided with, is available for sliding block 612 reciprocal
The chute of motion, each sliding block 612 is slidably mounted in chute, and spring is connected between each sliding block 612 and grip slipper 611
613, each front end of sliding block 612 is connected with upper driven pulley 614, the ware seat of upper driven pulley 614 and culture dish 31 by a connecting rod
311 are in contact, the purpose of the extensional motion of spring 613 design be in order that the spacing between two upper driven pulleys 614 can adjust,
To adapt to the diameter of culture dish 31；Described fore-clamp unit 62 include clamping push rod 621, gripper jaw 622, lower driving wheel 623, under
Driven pulley 624 and clamping motor 625, described clamping push rod 621 are arranged on body 1, and clamping push rod 621 end is fixed with folder
Pawl 622 is held, the madial wall of gripper jaw 622 is provided with for the lower driving wheel 623 and lower driven pulley with the contact of the ware of culture dish 31 seat 311
624, described clamping motor 625 is arranged on gripper jaw 622 and connects lower driving wheel 623.During work, clamping push rod 621 is stretched
Go out a certain distance and push culture dish 31 to upper driven pulley 614, culture dish 31 lower driving wheel 623, lower driven pulley 624 and on from
It is clamped under the collective effect of driving wheel 614, in plate streaking, the contact culture medium 313 of microbionation ring 473 is motionless, then presss from both sides
The lower clockwise movement of driving wheel 623 of the driving of pawl 622 is held, now culture dish 31 can be rotated and then, and microbionation ring 473 is drawn
After line, line certain distance, lower driving wheel 623 stops operating, and microbionation ring 473 rises, the lower rotate counterclockwise of driving wheel 623
Culture dish 31 is set to be returned to initial position, clamping push rod 621 is retracted a certain distance backward, to carry out the line of second segment then
Repeat the above steps progress third time line, and three sections of lines complete to rule for the first time after having drawn, and then microbionation ring 473 rises
And reach sterilization station and sterilized, while clamping push rod 621 and stretching out makes culture dish 31 be returned to initial position, then clamping dress
6 cooperation, three microbionation rings 473 are put while ruling to complete second of line, are sterilized after the completion of second of line, weight
Multiple second of action carries out third time line, until line is completed.
As shown in Figure 1, Figure 2, shown in Fig. 4 and Fig. 5, a kind of bacterium automatic vaccination instrument of the present invention, described takes lid arrangement 7
Including taking lid rotating disk 71, taking lid bar 72, take lid motor 73, take cover plate 74 and take lid terminal 75, described takes lid rotating disk 71
On body 1, described takes lid bar 72 installed in taking in lid rotating disk 71, and it is automatic telescopic rod member to take lid bar 72, takes lid
The upper end of bar 72, which is provided with, takes lid motor 73, described to take lid motor 73 to be servomotor, described to take cover plate 74 to be horizontally arranged to take
On lid motor 73, take the lower end of cover plate 74 to be provided with and take lid terminal 75；Described takes lid terminal 75 to include two horizontal reversed arrangements
Lid push rod 741, grip block 742, elastomeric pad 743 and pressure sensor 744, described clamping are taken take the lower surface of cover plate 74
Plate 742 is connected in and respectively takes the front end of lid push rod 741, and described elastomeric pad 743 is arranged on the inner side of grip block 742 and is used for and ware lid
312 are in contact, and pressure sensor 744, the described heatproof of elastomeric pad 743 are provided between elastomeric pad 743 and grip block 742
Height, the purpose that elastomeric pad 743 is designed is to cause in order to avoid the temperature due to ware lid 312 is too high to pressure sensor 744
Cause to damage.During work, cover plate 74 is taken to be located at directly over culture dish 31, taking cover plate 74 to decline makes ware lid 312 take lid positioned at two
Between terminal 75, then take lid push rod 741 to bounce back and clamp ware lid 312, then take lid bar 72 rise by ware lid 312 from ware seat
311 remove, after ware lid 312 reaches certain altitude, and taking lid motor 73 to rotate counterclockwise certain angle makes ware lid 312 be located at sterilizing
The top of device 5 sterilizes to ware lid 312, just can now carry out inoculation line work, after the completion of inoculation, take the up time of lid motor 73
Pin rotate, make ware lid 312 be located at ware seat 311 immediately below, then take lid bar 72 to rise, be next time take lid to prepare.
During work, first, the test tube that will be equipped with bacterial solution by artificial or machinery equipment is sequentially placed into test tube storage
In device 2, then, then it will be equipped with the culture dish 31 of culture medium 313 and be sequentially placed into culture dish conveyer 3；When needs are carried out carefully
When bacterium is inoculated with, classification inoculation apparatus 4 is located at the top of bactericidal unit 5 and microbionation ring 473 is sterilized, after the completion of sterilizing, sterilizing dress
5 extinguishings are put, inoculation rotating disk 41 is rotated clockwise, and in rotation process, an infrared distance sensor 46 detects an inspection
Measuring point 23, now microbionation ring 473 is inoculated with the decline of bar 42 shortening oese 473 directly over test tube, then and bacterium is molten
The distance of liquid, then takes bacterium electric pushrod 45 to decline and bacterial solution is dipped, then take bacterium electric pushrod 45 to retract, inoculation
Bar 42, which rises, makes microbionation ring 473 leave test tube, is then inoculated with rotating disk 41 and rotates counterclockwise, No. two infrared distance sensors
48 detect No. three test points 54, and now microbionation ring 473 is located at directly over culture dish, and inoculation rotating disk 41 stops operating,
Inoculation bar 42 declines, and disinfection inoculation device 4 is carried out to surrounding environment and takes lid arrangement 7 to carry out simultaneously while bactericidal unit 5 is lighted,
Cover plate 74 is taken to be located at directly over culture dish 31, taking cover plate 74 to decline makes 611 to be taken positioned at two between lid terminal 75, then takes lid to push away
Bar 741, which bounces back, clamps grip slipper 611, then takes lid bar 72 to rise and removes ware lid 312 from ware seat 311, when ware lid 312 is reached
After certain altitude, taking lid motor 73 to rotate counterclockwise certain angle makes ware lid 312 enter positioned at the top of bactericidal unit 5 to ware lid 312
Row sterilizing, just can now carry out the first time line of microbionation ring 473, and after the completion of ruling for the first time, inoculation bar 42 rises, and revolves
Bar motor 43 is rotated clockwise, and No. two infrared distance sensors 48 detect No. two test points 53, now 473, microbionation ring
Directly over bactericidal unit 5, the stretching of two microbionation rings 473 and the microbionation ring 473 of the leftmost side positioned at right are kept
Unanimously, now three microbionation rings 473 of bactericidal unit 5 pair sterilize simultaneously, and after the completion of sterilizing, swing arm motor 43 is counterclockwise
Rotate and return to scribing position by No. two infrared sensors 48 and carry out second of line, three microbionation rings 473 are in clamping
Rule simultaneously under the cooperation of device 6, then repeat above-mentioned action and carry out third time line, until line is completed, terminate to repeat dynamic
Make, after the completion of inoculation, take lid motor 73 to rotate clockwise, grip slipper 611 is located at immediately below ware seat 311, then clamp push rod
621 stretching a certain distance push culture dish 31 to upper driven pulley 614, and culture dish 31 is in lower driving wheel 623, lower driven pulley 624
It is clamped with the collective effect of upper driven pulley 614, in plate streaking, the contact culture medium 313 of microbionation ring 473 is motionless,
Then gripper jaw 622 drives the lower clockwise movement of driving wheel 623, and now culture dish 31 can be rotated and then, and microbionation ring 473 enters
After row line, line certain distance, lower driving wheel 623 stops operating, and microbionation ring 473 rises, and lower driving wheel 623 is counterclockwise
Rotation makes culture dish 31 be returned to initial position, and clamping push rod 621 is retracted a certain distance backward, to carry out the line of second segment
Then repeat the above steps progress third time line, and three sections of lines complete to rule for the first time after having drawn, then microbionation ring 473
Rise and reach sterilization station and sterilized, while clamping push rod 621 and stretching out makes culture dish 31 be returned to initial position, then pressed from both sides
Hold device 6 and coordinate three microbionation rings 473 while ruling to complete second of line, gone out after the completion of second of line
Bacterium, repeats second of action and carries out third time line, until line is completed, after line is finished, by ware lid 312 in clamping device
Cooperation under be placed on ware seat 311 on, then the culture dish 31 for being inoculated with completion is transported to labelling module by conveyer belt 32 again
Labelled at 33, and then complete whole plane system for automatic marker making process.
The basic principles, principal features and advantages of the present invention have been shown and described above.The technical staff of the industry should
Understand, the present invention is not limited to the above embodiments, the simply explanation present invention of the description in above-described embodiment and specification
Principle, without departing from the spirit and scope of the present invention, various changes and modifications of the present invention are possible, and these change and changed
Enter all fall within the protetion scope of the claimed invention.The claimed scope of the invention is by appended claims and its equivalent
Define.
Claims (6)
1. a kind of bacterium automatic vaccination instrument, it is characterised in that：Including body (1), test tube storing unit (2), culture dish transmission dress
Put (3), classification inoculation apparatus (4), bactericidal unit (5), clamping device (6) and take the test tube storing unit (2) described in lid arrangement (7) to pacify
Mounted in body (1) rear portion and fill germy test tube for storage；Described culture dish conveyer (3) is deposited installed in test tube
In front of device (2) and for transporting culture dish；Described classification inoculation apparatus (4) is arranged on test tube storing unit (2) right, inoculation dress
Putting (4) is used to dip the bacterium in test tube storing unit (2), and bacterium is transported into bactericidal unit (5) place, and in bactericidal unit
(5) place carries out plate streaking to culture dish；Described bactericidal unit (5) is arranged on body (1) and transmits dress positioned at culture dish
Put directly over (3), bactericidal unit (5) is used to play during microbionation to surrounding environment and go out to classification inoculation apparatus (4)
Bacterium is acted on, and described clamping device (6) is arranged on body (1), and for clamping culture dish, described takes lid arrangement (7) to pacify
On body (1) and for carrying out taking lid to culture dish (31)；Wherein：
Described bactericidal unit (5) is including a sterilization unit (51) for being sterilized to microbionation ring, positioned at No. one
No. two sterilization units (52) of sterilization unit (51) right, No. two test points (53) in front of a sterilization unit (51) and
No. three test points (54) directly over the culture dish that will be rule, a sterilization unit (51) and No. two sterilization units (52)
On be mounted on flame spray head for sterilizing.
2. a kind of bacterium automatic vaccination instrument according to claim 1, it is characterised in that：Described test tube storing unit (2)
On be evenly arranged with and deposit pore (21) for deposit test tube, respectively deposit and rubber blanket (22) be equipped with pore (21), test tube is laid
In the rubber blanket (22) of pore (21) is deposited, and respectively deposit pore (21) place be mounted on be used for detect whether classification inoculation apparatus (4) arrives
The test point (23) come；Shock module (24), vibration module (24) are also equipped with the middle part of described test tube storing unit (2)
It for producing micro-vibration, on the one hand can prevent bacterial solution from precipitating, on the other hand be conducive to the accurate sampling of classification inoculation apparatus 4.
3. a kind of bacterium automatic vaccination instrument according to claim 1, it is characterised in that：Described culture dish conveyer
(3) culture dish (31), conveyer belt (32) and labelling module (33) are included, described culture dish (31) includes ware seat (311), ware
Lid (312), and the culture medium (313) being placed in ware seat (311), culture dish (31) are uniformly placed on conveyer belt (32)；Institute
The conveyer belt (32) stated is arranged on body (1) and for culture dish (31) to be transported into classification inoculation apparatus (4) scribing position just
Lower section；Described labelling module (33) is arranged on body (1) and for being labelled to culture medium (313).
4. a kind of bacterium automatic vaccination instrument according to claim 1, it is characterised in that：Described classification inoculation apparatus (4) includes
Inoculation rotating disk (41), inoculation bar (42), swing arm motor (43), inoculation transverse slat (44), take bacterium electric pushrod (45), it is No. one infrared
Range sensor (46) and bacterium module (47) is taken, described inoculation rotating disk (41) is arranged on body (1), inoculation bar (42) is
Automatic telescopic link and in inoculation rotating disk (41), described swing arm motor (43) is arranged on inoculation bar (42) upper end, institute
The inoculation transverse slat (44) stated is horizontally arranged at swing arm motor (43) upper end, and transverse slat (42) front end is vertically-mounted takes bacterium electronic for inoculation
Push rod (45), No. two described infrared sensor test points (46) are arranged on inoculation transverse slat (44), to realize classification inoculation apparatus (4)
Positioning during sampling；Described takes bacterium module (47) installed in bacterium electric pushrod (45) lower end is taken, and takes bacterium module (47) to include solid
Determine block (471), lifting module (472), microbionation ring (473) and No. three infrared ray range sensors (474), described fixation
Block (471) weldering, which is connected in, to be taken on bacterium electric pushrod (45), and described microbionation ring (473) quantity is three, and two of which bacterium connects
Plant ring (473) to be arranged on fixed block (471) by two lifting modules (472) respectively, respectively lifting module (472) can free band
Dynamic each microbionation ring (473) is moved up and down, and No. three described infrared ray range sensors (474) are arranged on the right side of fixed block,
Accurately bacterium is dipped for detection bacterium oese (473).
5. a kind of bacterium automatic vaccination instrument according to claim 1, it is characterised in that：Described clamping device (6) includes
Unit (61) and fore-clamp unit (62) are pressed from both sides afterwards, and described rear folder unit (61) is installed on body (1) with fore-clamp unit (62)
And positioned at culture dish conveyer (3) front and rear sides；Folder unit (61) includes grip slipper (611), two sliding blocks (612), bullets afterwards
It is provided with the middle part of spring (613) and upper driven pulley (614), grip slipper (611) and is available for two reciprocating chutes of sliding block (612),
Each sliding block (612) is slidably mounted in chute, and spring (613) is connected between each sliding block (612) and grip slipper (611),
Each sliding block (612) front end is connected with upper driven pulley (614), upper driven pulley (614) and culture dish (31) by a connecting rod
Ware seat 311 is in contact；Described fore-clamp unit (62) include clamping push rod (621), gripper jaw (622), lower driving wheel (623),
Lower driven pulley (624) and clamping motor (625), described clamping push rod (621) are arranged on body (1), clamp push rod (621)
End is fixed with gripper jaw (622), and gripper jaw (622) madial wall is provided with for under the contact of culture dish (31) ware seat 311
Driving wheel (623) and lower driven pulley (624), described clamping motor (625) are arranged on gripper jaw (622) and connect lower active
Take turns (623).
6. a kind of bacterium automatic vaccination instrument according to claim 1, it is characterised in that：Described takes lid arrangement (7) to include
Lid rotating disk (71) is taken, lid bar (72) is taken, takes lid motor (73), takes cover plate (74) and take lid terminal (75), described takes lid to revolve
Rotating disk (71) is arranged on body (1), and described takes lid bar (72) installed in taking in lid rotating disk (71), and it is certainly to take lid bar (72)
Dynamic telescoping rod, takes lid bar (72) upper end to be provided with and takes lid motor (73), described to take cover plate (74) to be horizontally arranged to take lid electricity
On machine (73), take cover plate (74) lower end to be provided with and take lid terminal (75)；Described takes lid terminal (75) reverse including two levels
Be arranged in take cover plate (74) lower surface take lid push rod (741), grip block (742), elastomeric pad (743) and pressure sensor
(744), described grip block (742) is connected in and respectively takes lid push rod (741) front end, and described elastomeric pad (743) is arranged on folder
Hold on the inside of plate (742) and for being in contact with ware lid (312), pressure is installed between elastomeric pad (743) and grip block (742)
Sensor (744).
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| CN201710314288.3A CN107118950B (en) | 2017-05-06 | 2017-05-06 | Automatic bacterial inoculation instrument |
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| CN107118950B CN107118950B (en) | 2019-10-11 |
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