CN107418888A - A kind of cyclic microorganism classification inoculation apparatus and its inoculation method - Google Patents
A kind of cyclic microorganism classification inoculation apparatus and its inoculation method Download PDFInfo
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- CN107418888A CN107418888A CN201710883394.3A CN201710883394A CN107418888A CN 107418888 A CN107418888 A CN 107418888A CN 201710883394 A CN201710883394 A CN 201710883394A CN 107418888 A CN107418888 A CN 107418888A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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Abstract
The present invention relates to medical science, and in particular to a kind of cyclic microorganism classification inoculation apparatus and its inoculation method.Cyclic microorganism classification inoculation apparatus provided by the invention, rotating mechanism is set on base and be sequentially arranged and the rotating mechanism sample unit, culture dish controlling organization unit, sterilization unit and the temperature reducing unit that are used cooperatively, the circulate operation for making the multiple inoculation bars set on rotating mechanism be sampled, be inoculated with, sterilize and cool respectively can be achieved, therefore, microbial inoculant is carried out using this device can be uninterruptedly inoculated with the multi partition of a culture dish.The circulation inoculation apparatus structure of the present invention is simple, easy to operate, and inoculation efficiency is high, disclosure satisfy that the demand of clinically large sample size；Meanwhile plant automation degree is high, working specification is inoculated with, further ensures experimenter and the safety of biology.
Description
Technical field
The present invention relates to medical science, and in particular to a kind of cyclic microorganism classification inoculation apparatus and its inoculation method.
Background technology
At present in microorganism industry, the microbionation to solid medium mainly also has been manually done by people, with subarea
Exemplified by, it, which is operated, needs first to pick sample with oese, then carries out the inoculation in the firstth area on culture medium again, then by oese
Sterilized 5 seconds on alcolhol burner, remove alcolhol burner and cool down 10 seconds, then carry out the inoculation of the secondth area, then by oese in alcolhol burner
Upper sterilizing 5 seconds, remove alcolhol burner and cool down 10 seconds, then carry out the inoculation of the 3rd area.Due to manual operation cause working specification and
Bio-safety can not be effectively guaranteed.
Nearest 2 years from French Mei Liai biotech firms and Siemens respectively to market be proposed it is respective from
Dynamic inoculation product, its operational standardization and biological safety have obtained the welcome and accreditation in market, but due in microbial inoculant
During need repeatedly high-temperature sterilization and cooling are carried out to oese, waste the plenty of time, cause instrument inoculation efficiency not
Height, sample size demand clinically can not be met.
The content of the invention
For background technology problem encountered, it is an object of the invention to provide a kind of cyclic microorganism classification inoculation apparatus and
Its inoculation method, microbial inoculant efficiency is greatly improved on the premise of standard operation is ensured, further meet clinically full-page proof
The demand of this amount.
In order to achieve the above object, the present invention adopts the following technical scheme that：
The invention provides a kind of cyclic microorganism classification inoculation apparatus, including base, it is provided with the base for being inoculated with
The rotating mechanism of sample transmission and be sequentially arranged and the rotating mechanism sample unit, the culture dish controlling organization that are used cooperatively
Unit, sterilization unit and temperature reducing unit.
As the further scheme of the present invention, the rotating mechanism includes the first rotating stepper motor and installed in the first rotation
Turn the rotation inoculation disk in stepper motor rotary shaft, 4 inoculation bars are averagely arranged on the rotation inoculation disk；The sample cup,
Culture dish controlling organization, bactericidal unit and heat sink are separately positioned on position corresponding with 4 inoculation bars.
As the further scheme of the present invention, the culture dish controlling organization unit includes：Culture dish, the first slide unit and
Second for making the first motor, the second slide unit that culture dish slides on the first slide unit and making that culture dish slides on the second slide unit
Motor；Second slide unit is fixedly connected on the first slide unit, and the first slide unit and the orthogonal composition culture dish of the second slide unit are in X
Movement locus on axle and Y-axis；The second rotating stepper motor is fixedly connected with first slide unit, the second rotating stepper motor
Culture dish is set in rotary shaft.
As the further scheme of the present invention, culture dish support is fixedly connected with the rotary shaft of second rotating stepper motor
Frame, the culture dish are placed in culture dish support frame.
As the further scheme of the present invention, the sterilization unit is alcolhol burner sterilizing, Bunsen burner sterilizing, infrared ray sterilizing
Any of device sterilizing.
As the further scheme of the present invention, the infrared ray sterilizer is slidably connected on a slide unit, red for adjusting
The height of outside line sterilizer.
Present invention also offers a kind of cyclic microorganism inoculation method based on above-mentioned cyclic microorganism classification inoculation apparatus, tool
Body comprises the following steps：
A) rotation inoculation disc spins make the first inoculation bar pick the sample in sample cup in sample unit；
B) after the first inoculation bar picks sample, rotation inoculation disc spins make the first inoculation bar rotate to culture dish controlling organization
The culture dish surface of unit, carry out first by the aggregate motion of the first slide unit, the second slide unit and the second rotating stepper motor and connect
Kind bar is inoculated with the firstth area of culture dish；Meanwhile second is inoculated with bar in sample unit completion sampling；
C) after the first inoculation bar completes inoculation, the second inoculation bar completes sampling, rotation inoculation disc spins make the first inoculation bar
Rotation to sterilization unit, the second inoculation bar is rotated to inoculation unit, completes to exist to the first sterilizing for being inoculated with bar and the second inoculation bar
The inoculation in the secondth area of culture dish, meanwhile, the 3rd inoculation bar is completed to sample in sample unit；
D) after the first inoculation bar completes sterilizing, the second inoculation bar completes inoculation, the 3rd inoculation bar completes sampling, rotation inoculation
Disc spins make the first inoculation bar rotate to temperature reducing unit, the second inoculation bar to rotate to sterilization unit, the 3rd inoculation bar and rotate to connecing
Kind unit, the cooling to the first inoculation bar, the sterilizing to the second inoculation bar and the 3rd inoculation bar are completed in the 3rd area of culture dish
Inoculation, meanwhile, the 4th inoculation bar sample unit complete sample.
As the further scheme of the present invention, 4 inoculation bars on rotation inoculation disk complete sampling, inoculation, sterilizing and drop
After one wheel inoculation experiments operation of temperature, the inoculation experiments operation of operation next round is continued cycling through.
Beneficial effects of the present invention：The circulation inoculation apparatus structure of the present invention is simple, and easy to operate, its important feature is
Multiple inoculation bars are set on a rotating mechanism, and what each inoculation bar can respectively be sampled, and be inoculated with, sterilize and cool follows
Ring operates, and inoculation efficiency is high, disclosure satisfy that the demand of clinically large sample size；Meanwhile plant automation degree is high, inoculation operation
Specification, further ensure experimenter and the safety of biology.
Brief description of the drawings
Fig. 1 is the structural representation of the cyclic microorganism classification inoculation apparatus in the embodiment of the present invention；
Fig. 2 is rotation inoculation dish structure schematic diagram in the embodiment of the present invention；
Fig. 3 is culture dish controlling organization cellular construction schematic diagram in the embodiment of the present invention；
Fig. 4 is infrared ray sterilizer structural representation in the embodiment of the present invention；
Fig. 5 is that bar and the structural representation of rotation inoculation disk connected mode are inoculated with the embodiment of the present invention.
In figure：1- rotation inoculations disk, 2- sample cups, 3- culture dish controlling organizations unit, 4- infrared rays sterilizing mechanism, 5- drops
Warm air fan, 6- bases, the rotating stepper motors of 7- first, 8- inoculations bar, 9- culture dishes, the rotating stepper motors of 10- second, 11- the
Two slide units, the slide units of 12- first, 13- culture dish support frames, the sliding motors of 14- first, the sliding motors of 15- second, 16- infrared rays go out
Bacterium device, 17- sterilizers slide unit, 18- inoculation bar cramps head, 19- inoculation bars fixed seat, 20- rotary pin shafts
Embodiment
In order to be better understood from the purpose of the present invention, structure, feature and effect etc., in conjunction with accompanying drawing and specific embodiment party
The invention will be further described for formula, and embodiment should not be construed as limiting the scope of the present invention.Can to present disclosure
To be improved from material, method and reaction condition simultaneously, all these improvement, spirit and scope of the invention all should be fallen into
Within.
Embodiment 1：Cyclic microorganism classification inoculation apparatus
As shown in figure 1, a kind of cyclic microorganism classification inoculation apparatus, including base 6, it is provided with base for seed sample
And sample bar transmission rotating mechanism and be sequentially arranged, and rotating mechanism be used cooperatively sample cup 2, culture dish control machine
Structure unit 3, infrared ray sterilizing mechanism 4 and cooling fan 5.As shown in Fig. 2 wherein rotating mechanism includes the first rotating stepper motor
7 and in the first rotating stepper motor rotary shaft rotation inoculation disk 1, rotation inoculation disk 1 on averagely arrange 4 inoculation bars
8, sample cup 2, culture dish controlling organization unit 3, infrared ray sterilizing mechanism 4 and cooling fan 5 are separately positioned on and 4 inoculation bars
On corresponding position；As shown in figure 3, culture dish controlling organization unit 3 includes culture dish 9, the first slide unit 12 and makes culture dish
The first sliding motor 14 for being slided on the first slide unit 12, the second slide unit 11 and make what culture dish slided on the second slide unit 11
Second sliding motor 15；Second slide unit 11 is fixedly connected on the first slide unit 12, and the first slide unit 12 and the second slide unit 11 are mutual
Movement locus of the perpendicular composition culture dish in X-axis and Y-axis；The second rotation stepping electricity is fixedly connected with first slide unit 12
Machine 10, sets culture dish support frame 13 in the rotary shaft of the second rotating stepper motor 10, culture dish holding 9 on culture dish support frame 13,
Culture dish controlling organization unit 3 can make culture dish 9 move in X-axis, Y direction, while can also make culture dish any rotation
To complete to be inoculated with inoculation of the bar in culture dish.As shown in figure 4, the infrared ray sterilizing mechanism 4 in the present embodiment is one can be
The infrared ray sterilizer 16 slided up and down in sterilizer slide unit 17, infrared ray can be adjusted according to the height for the inoculation bar that need to be sterilized
The height of sterilizer.The present embodiment is that inoculation bar 8 is sterilized using infrared ray, but is not limited thereto, and can also use and compare biography
System as alcolhol burner sterilizing, Bunsen burner sterilizing the methods of.
Preferably, when the rotation inoculation disk of rotating mechanism rotates, after inoculation bar sampling behind rotation to the position of culture dish,
In order to avoid inoculation bar scratches culture medium, and it is inoculated with bar and is preferably positioned in culture dish, one can be set on base 6
Frame, the inoculation support 18 of other end connection one of support, inoculation support 18 are positioned close to the position of culture dish controlling organization unit 3
Put；Corresponding, inoculation bar 8 is arranged on the rotation in the inoculation bar fixed seat 19 and fixed seat on rotation inoculation disk 1 by one
Bearing pin 20 can freely adjust in seeded process with the height change of media surface, and it is applied to the power of media surface
Degree can keep constant.
Embodiment 2：The cyclic microorganism inoculation method of classification inoculation apparatus based on embodiment one
Be inoculated with disk 1 around rotation, the positions of corresponding 4 inoculation bars be respectively disposed with sample cup 2, culture dish motion 2,
Infrared sterilizing mechanism 4 and cooling fan 5.
The cyclic microorganism inoculation method of cyclic microorganism classification inoculation apparatus based on embodiment 1, concrete operations are as follows：
Sample cup is driven first with first with manually or mechanically hand, first inoculation bar is picked in sample cup
Sample.
Then the first rotating stepper motor 7 drives rotating disk 1 to rotate counterclockwise 90 degree, first inoculation bar is reached culture
The surface of ware 9, at this moment pass through the combination of the first slide unit of culture dish 12, culture dish the second slide unit 11 and the second rotating stepper motor 10
Motion realizes that first inoculation bar is inoculated with the firstth area of culture dish 9；Second inoculation bar is also completed in sample cup simultaneously
Sampling.
First rotating stepper motor 7 drives rotating disk 1 to rotate counterclockwise 90 degree, second inoculation bar is reached culture dish 9
Surface carry out the inoculation of the secondth area, while first inoculation bar reaches the top of infrared ray sterilizing mechanism 4, infrared sterilizer motion
Slide unit 17 drives infrared sterilizer 16, and motion makes first inoculation bar enter in sterilizing hole the high-temperature sterilization carried out 9 seconds upwards,
Then infrared sterilizer up and down motion slide unit 17 drives infrared sterilizer 16 to move downward completion sterilizing；Meanwhile second is inoculated with bar
The inoculation in the secondth area in culture dish is completed, the 3rd inoculation bar completes sampling.
Last first rotating stepper motor 7 drives rotating disk 1 to rotate counterclockwise 90 degree, the 3rd inoculation bar is reached culture
The surface of ware 9 carries out the inoculation of the 3rd area, the second inoculation bar is reached the top of infrared ray sterilizing mechanism 4 and is sterilized, makes first
Individual inoculation bar carries out fast cooling before reaching cooling fan 5, can so complete the inoculated of three subregion within a short period of time
Journey.
4 inoculation bars on rotation inoculation disk in technical scheme complete sampling, inoculation, sterilizing and cooling
After one wheel inoculation experiments operation, the inoculation experiments operation of operation next round is continued cycling through.
The foregoing is only a preferred embodiment of the present invention, but protection scope of the present invention be not limited thereto,
Any one skilled in the art the invention discloses technical scope in, technique according to the invention scheme and its
Inventive concept is subject to equivalent substitution or change, should all be included within the scope of the present invention.
Claims (8)
1. a kind of cyclic microorganism classification inoculation apparatus, including base, it is characterised in that be provided with the base for seed sample
The rotating mechanism of transmission and be sequentially arranged, the sample unit that is used cooperatively with rotating mechanism, culture dish controlling organization unit,
Sterilization unit and temperature reducing unit.
A kind of 2. microbe inoculating device according to claim 1, it is characterised in that：The rotating mechanism includes the first rotation
Turn stepper motor and the rotation inoculation disk in the first rotating stepper motor rotary shaft, the rotation is inoculated with average cloth on disk
Put 4 inoculation bars；The sample cup, culture dish controlling organization, bactericidal unit and heat sink are separately positioned on and 4 inoculation bars
Corresponding position.
A kind of 3. microbe inoculating device according to claim 1, it is characterised in that：The culture dish controlling organization unit
Including：Culture dish, the first slide unit and make the first motor, the second slide unit and make culture that culture dish slides on the first slide unit
The second motor that ware slides on the second slide unit；Second slide unit is fixedly connected on the first slide unit, the first slide unit and second
Movement locus of the orthogonal composition culture dish of slide unit in X-axis and Y-axis；The second rotation step is fixedly connected with first slide unit
Stepper motor, culture dish is set in the rotary shaft of the second rotating stepper motor.
A kind of 4. microbe inoculating device according to claim 3, it is characterised in that：Second rotating stepper motor
Culture dish support frame is fixedly connected with rotary shaft, the culture dish is placed in culture dish support frame.
A kind of 5. microbe inoculating device according to claim 1, it is characterised in that：The sterilization unit goes out for alcolhol burner
Any of bacterium, Bunsen burner sterilizing, the sterilizing of infrared ray sterilizer.
A kind of 6. microbe inoculating device according to claim 5, it is characterised in that：The infrared ray sterilizer, which slides, to be connected
It is connected on a slide unit, for adjusting the height of infrared ray sterilizer.
A kind of 7. cyclic microorganism inoculation method of the cyclic microorganism classification inoculation apparatus based on claim 1, it is characterised in that：Bag
Include following steps：
A) rotation inoculation disc spins make the first inoculation bar pick the sample in sample cup in sample unit；
B) after the first inoculation bar picks sample, rotation inoculation disc spins make the first inoculation bar rotate to culture dish controlling organization unit
Culture dish surface, the first inoculation bar is carried out by the aggregate motion of the first slide unit, the second slide unit and the second rotating stepper motor
It is inoculated with the firstth area of culture dish；Meanwhile second is inoculated with bar in sample unit completion sampling；
C) after the first inoculation bar completes inoculation, the second inoculation bar completes sampling, rotation inoculation disc spins rotate the first inoculation bar
Rotated to sterilization unit, the second inoculation bar to inoculation unit, complete cultivating the first sterilizing for being inoculated with bar and the second inoculation bar
The inoculation in the secondth area of ware, meanwhile, the 3rd inoculation bar is completed to sample in sample unit；
D) after the first inoculation bar completes sterilizing, the second inoculation bar completes inoculation, the 3rd inoculation bar completes sampling, rotation inoculation is spiraled
Turn to make the first inoculation bar rotate to temperature reducing unit, the second inoculation bar rotate to sterilization unit, the 3rd inoculation bar rotate it is single to inoculation
Member, complete the cooling to the first inoculation bar, the sterilizing to the second inoculation bar and the 3rd inoculation bar connecing in the 3rd area of culture dish
Kind, meanwhile, the 4th inoculation bar is completed to sample in sample unit.
8. a kind of cyclic microorganism of cyclic microorganism classification inoculation apparatus based on claim 1 according to claim 7 connects
Kind method, it is characterised in that rotate the wheel that 4 inoculation bars being inoculated with disk are completed to sample, be inoculated with, sterilize and cool and be inoculated with fact
After testing operation, the inoculation experiments operation of operation next round is continued cycling through.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710883394.3A CN107418888A (en) | 2017-09-26 | 2017-09-26 | A kind of cyclic microorganism classification inoculation apparatus and its inoculation method |
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| CN201710883394.3A CN107418888A (en) | 2017-09-26 | 2017-09-26 | A kind of cyclic microorganism classification inoculation apparatus and its inoculation method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110982679A (en) * | 2020-01-08 | 2020-04-10 | 台州黄岩博友智能技术有限公司 | Inoculation device for microbiological assay |
| CN113355218A (en) * | 2020-03-05 | 2021-09-07 | 房本院 | Full-automatic blood bacteria culture appearance of screw propulsion formula for clinical laboratory of hospital |
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| US20040064013A1 (en) * | 2001-02-09 | 2004-04-01 | Daniel Attias | Incubator and incubation method monitoring the organism to be incubated |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113355218A (en) * | 2020-03-05 | 2021-09-07 | 房本院 | Full-automatic blood bacteria culture appearance of screw propulsion formula for clinical laboratory of hospital |
| CN113355218B (en) * | 2020-03-05 | 2022-07-12 | 上海揽微赛尔生物科技有限公司 | Full-automatic blood bacteria culture appearance of screw propulsion formula for clinical laboratory of hospital |
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