CN106879275A - A kind of quick method for effectively improving Hainan Chinese scholartree percentage of seedgermination - Google Patents
A kind of quick method for effectively improving Hainan Chinese scholartree percentage of seedgermination Download PDFInfo
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- CN106879275A CN106879275A CN201710018577.9A CN201710018577A CN106879275A CN 106879275 A CN106879275 A CN 106879275A CN 201710018577 A CN201710018577 A CN 201710018577A CN 106879275 A CN106879275 A CN 106879275A
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- seed
- hainan
- chinese scholartree
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- seedses
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a kind of quick method for effectively improving Hainan Chinese scholartree percentage of seedgermination.A, the ripening fruits of collection Hainan Chinese scholartree are dried naturally, and Fruit pod is gone in stranding, then exposure 16 24 hours in the sun so that the control of the storage of seeds moisture obtains stored seedses in 9% 12%;B, stored seedses are sterilized after, avoid one openning of kind skin that embryo position cuts seed, be soaked in 23 days in seed treatment, obtain Hainan Chinese scholartree seed of imbibition, every liter described of seed treatment contains:The 100mg of GA3 50, the 0.1mg of 6 BA 0.05,170 340mg potassium dihydrogen phosphates, remaining is water;C, by Hainan Chinese scholartree seed broadcasting of imbibition in the river sand that water content is 20 25%, and cover seed with river sand.The present invention promotes seed to sprout, and can effectively improve the speed of germinating and germination percentage of Hainan Chinese scholartree seed.The seed of Hainan Chinese scholartree is processed using chemical substance, the activity of Their Seed Germinating Period enzyme is have activated, accelerates the metabolism of matter and energy, simple and reliable process, it is rapid effectively.
Description
Technical field
The invention belongs to field of plant reproduction, and in particular to a kind of quick side for effectively improving Hainan Chinese scholartree percentage of seedgermination
Method.
Background technology
Hainan Chinese scholartree also known as south of the Five Ridges Chinese scholartree, be Caesalpiniaceae (Caesalpiniaceae) Sophora (Sophora) perennial shrub or
Dungarunga, is that the peculiar Wild Ornamental class plant in Hainan and feed use plant.Hainan Chinese scholartree has stronger resistance and Saline alkali tolerance,
It is Hainan indigenous tree, is especially suitable for the planting technique and coastal city afforestation of the artificial hydraulic reclamation islands and reefs in China South Sea, has
Economic benefit very high, social benefit and ecological benefits.But gone to breed Hainan Chinese scholartree at present with seed, the germination rate of its seed is non-
It is often low, only 10% or so, this has had a strong impact on Hainan Chinese scholartree large-scale breeding.
The content of the invention:
It is an object of the invention to provide a kind of method that can quickly effectively improve Hainan Chinese scholartree percentage of seedgermination, using the present invention
Method can increase substantially the germination percentage of Hainan Chinese scholartree seed.
Then the present invention is cut kind of a skin using sharp scalpel by removal Hainan Chinese scholartree seed top layer miscellaneous bacteria of sterilizing, and is connect
And soaked with seed treatment again, being broadcast finally by sand can significantly improve the germination percentage and germinating energy of Hainan Chinese scholartree seed,
And it is rapid, neat to emerge, growing way is consistent, it is achieved thereby that the purpose of the present invention.
The quick method for effectively improving Hainan Chinese scholartree percentage of seedgermination of the invention, it is characterised in that comprise the following steps:
A, the ripening fruits of collection Hainan Chinese scholartree are dried naturally, and Fruit pod is gone in stranding, then in the sun exposure 16-24 hours, is made
The control of the storage of seeds moisture is obtained in 9%-12%, stored seedses are obtained;
B, stored seedses are sterilized after, avoid one openning of kind skin that embryo position cuts seed, be soaked in seed
2-3 days in inorganic agent, Hainan Chinese scholartree seed of imbibition is obtained, every liter described of seed treatment contains:GA3 (gibberellin) 50-
100mg, 6-BA (6-benzyladenine) 0.05-0.1mg, 170-340mg potassium dihydrogen phosphate, remaining is water;
C, by Hainan Chinese scholartree seed broadcasting of imbibition in water content is for the river sand of 20-25%, and cover seed with river sand.
Described sterilization is that stored seedses are soaked 30 seconds in the alcohol water blend of volume fraction 70%, then uses mass fraction
0.1~0.3% potassium permanganate solution is sterilized 15~20 minutes, aseptic water washing 4~5 times, in aseptic bar on superclean bench
The moisture of the surface of the seed is blotted under part with aseptic filter paper.
Described stored seedses can be stored in 4 DEG C of refrigerators and preserve, and stored seedses are taken from refrigerator when to be used
Go out under the conditions of nature after being warming up to room temperature and carry out disinfection again.
Germination percentage is counted after 7d, Hainan Chinese scholartree percentage of seedgermination processed through the present invention is up to 92.1%, and control group (does not enter
Row kind of skin otch and clear water immersion) germination percentage only has 13.3%.
Hainan Chinese scholartree seed germination rate can be made to improve more than 70% using present invention treatment Hainan Chinese scholartree seed, and emerge it is rapid,
Neatly, growing way is consistent.Husky broadcasting sprouts the key technology emerged during the present invention can provide subset for Hainan Chinese scholartree industrial seedling rearing.This
Invention has simple to operate, low cost, step few, time-consuming short, the treatment effeciency of Hainan Chinese scholartree seed is remarkably improved, after treatment
Hainan Chinese scholartree germination is fast, seed bud quality is good, survival rate is high, the advantages of up to 92%.
The present invention first cuts one, Hainan Chinese scholartree kind skin (avoiding embryo position) osculum using physical method, by physics cutting
Mode destroys its kind of seal of skin so that Hainan Chinese scholartree seed can quickly absorb moisture, oxygen and seed by cutting part
Inorganic agent, so as to promote its seed to sprout, can effectively improve the speed of germinating and germination percentage of Hainan Chinese scholartree seed.Using chemical substance
To process the seed of Hainan Chinese scholartree, the activity of Their Seed Germinating Period enzyme is have activated, accelerate the metabolism of matter and energy, process is simple can
By, it is rapid effectively.
Specific embodiment:
Following examples are further illustrated to of the invention, rather than limitation of the present invention.
Embodiment 1:
1st, seed is gathered:It is strong robust growth, strong stress resistance, disease and insect resistance to be chosen from Wenchang, hainan island plant cultivation base
Hainan Chinese scholartree strain as seed collecting plant, with the ripening fruits of hand getting collection Hainan Chinese scholartree, be fitted into ventilative good seed collecting bag, it is natural
Dry, Fruit pod is gone in stranding, then in the sun exposure seed 16-24 hour, the control of the storage of seeds moisture is obtained in 9%-12%
Stored seedses, are loaded kraft paper bag by stored seedses, are placed in 4 DEG C of refrigerators and are preserved.
2nd, disinfection seed:Stored seedses being taken out from refrigerator after be warming up to room temperature under natural conditions, in volume fraction
Soaked 30 seconds in 70% alcohol water blend, then sterilized 20 minutes with the potassium permanganate solution of mass fraction 0.1%, aseptic water washing
5 times, in the moisture for blotting the surface of the seed under aseptic condition on superclean bench with aseptic filter paper, then cut with aseptic operation knife
Skin (avoiding embryo position) osculum is planted, in uniform broadcasting to sprouting culture dish, per (a diameter of 15 centimetres) placement seeds of ware
50.
3rd, seed treatment immersion:70 milliliters of seed treatment, room temperature (25 are poured into toward each culture dish for being covered with seed
2) DEG C ± immersion -3 days 2 days, obtain Hainan Chinese scholartree seed of imbibition.Every liter of seed treatment contains:GA3 50mg、6-BA
0.05mg, 170mg potassium dihydrogen phosphate, remaining is water, and specific compound method is by GA3 50mg, 6-BA 0.05mg, 170mg phosphorus
Acid dihydride potassium is dissolved into a small amount of water, is then settled to 1L with water, obtains seed treatment.
4th, the husky seed for broadcasting imbibition:It is that sand long 5 meters, wide 90 centimetres, deep 20 centimetres is set up in 90% cool canopy in shading rate
Pond, the clean river sand of a height of 15 centimetres of sand basin filling, 20-25% water content, Hainan Chinese scholartree seed of uniform broadcasting imbibition is in sand basin
On face, seed then is covered in spreading thickness 1cm river sands on seed face.Germination percentage is counted after 7d, through Hainan that the method is processed
Chinese scholartree percentage of seedgermination is up to 92.1%, control group (not carrying out kind of a skin otch, replace seed treatment to soak with clear water) germination percentage
Only 13.3%.
5th, upper cup is heeled in:Using 2.7 cun of white plastic cups, matrix is red soil, and when Hainan, Chinese scholartree seedling grows to two panels true leaf,
At 10-15 centimetres high, will be heeled in seedling immigration cup from sand basin, carried out normal water and fertilizer management.
Embodiment 2:
1st, seed is gathered:It is strong robust growth, strong stress resistance, disease and insect resistance to be chosen from Wenchang, hainan island plant cultivation base
Hainan Chinese scholartree strain as seed collecting plant, with the ripening fruits of hand getting collection Hainan Chinese scholartree, be fitted into ventilative good seed collecting bag, it is natural
Dry, Fruit pod is gone in stranding, then in the sun exposure seed 16-24 hour, the control of the storage of seeds moisture is obtained in 9%-12%
Stored seedses, are loaded kraft paper bag by stored seedses, are placed in 4 DEG C of refrigerators and are preserved.
2nd, disinfection seed:Stored seedses being taken out from refrigerator after be warming up to room temperature under natural conditions, in volume fraction
Soaked 30 seconds in 70% alcohol water blend, then sterilized 15 minutes with the potassium permanganate solution of mass fraction 0.3%, aseptic water washing
4 times, in the moisture for blotting the surface of the seed under aseptic condition on superclean bench with aseptic filter paper, then cut with aseptic operation knife
Skin (avoiding embryo position) osculum is planted, in uniform broadcasting to sprouting culture dish, per (a diameter of 15 centimetres) placement seeds of ware
50.
3rd, seed treatment immersion:80 milliliters of seed treatment, room temperature (25 are poured into toward each culture dish for being covered with seed
2) DEG C ± immersion -3 days 2 days, obtain Hainan Chinese scholartree seed of imbibition.Every liter of seed treatment contains:GA3 100mg、6-BA
0.1mg, 340mg potassium dihydrogen phosphate, remaining is water, and specific compound method is by GA3 100mg, 6-BA 0.1mg, 340mg phosphoric acid
Potassium dihydrogen is dissolved into a small amount of water, is then settled to 1L with water, obtains seed treatment.
4th, the husky seed for broadcasting imbibition:It is that sand long 5 meters, wide 90 centimetres, deep 20 centimetres is set up in 90% cool canopy in shading rate
Pond, the clean river sand of a height of 15 centimetres of sand basin filling, 20-25% water content, Hainan Chinese scholartree seed of uniform broadcasting imbibition is in sand basin
On face, seed then is covered in spreading thickness 1cm river sands on seed face.Germination percentage is counted after 7d, through Hainan that the method is processed
Chinese scholartree percentage of seedgermination is up to 93.1%, and control group (not carrying out kind of skin otch and clear water immersion) germination percentage only has 13.3%.
5th, upper cup is heeled in:Using 2.7 cun of white plastic cups, matrix is red soil, and when Hainan, Chinese scholartree seedling grows to two panels true leaf,
At 10-15 centimetres high, will be heeled in seedling immigration cup from sand basin, carried out normal water and fertilizer management.
Claims (3)
1. a kind of quick method for effectively improving Hainan Chinese scholartree percentage of seedgermination, it is characterised in that comprise the following steps:
A, the ripening fruits of collection Hainan Chinese scholartree are dried naturally, and Fruit pod is gone in stranding, then in the sun exposure 16-24 hour so that kind
Son storage moisture control obtains stored seedses in 9%-12%;
B, stored seedses are sterilized after, avoid one openning of kind skin that embryo position cuts seed, be soaked in Seed Treatment
2-3 days in agent, Hainan Chinese scholartree seed of imbibition is obtained, every liter described of seed treatment contains:GA3 50-100mg、6-BA
0.05-0.1mg, 170-340mg potassium dihydrogen phosphate, remaining is water;
C, by Hainan Chinese scholartree seed broadcasting of imbibition in water content is for the river sand of 20-25%, and cover seed with river sand.
2. method according to claim 1, it is characterised in that described sterilization is in volume fraction 70% by stored seedses
Soaked 30 seconds in alcohol water blend, then sterilized 15~20 minutes with the potassium permanganate solution of mass fraction 0.1~0.3%, it is aseptic
Water is rinsed 4~5 times, in the moisture for blotting the surface of the seed under aseptic condition on superclean bench with aseptic filter paper.
3. method according to claim 1, it is characterised in that described stored seedses are stored in 4 DEG C of refrigerators and preserve, and treat
Stored seedses being taken out from refrigerator when using carried out disinfection again after be warming up to room temperature under natural conditions.
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CN201710018577.9A CN106879275B (en) | 2017-01-10 | 2017-01-10 | Method for rapidly and effectively improving germination rate of sophora tonkinensis seeds |
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CN201710018577.9A CN106879275B (en) | 2017-01-10 | 2017-01-10 | Method for rapidly and effectively improving germination rate of sophora tonkinensis seeds |
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CN106879275A true CN106879275A (en) | 2017-06-23 |
CN106879275B CN106879275B (en) | 2020-11-17 |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107567756A (en) * | 2017-09-29 | 2018-01-12 | 南京仙草堂生物科技有限公司 | A kind of method for improving Chinese scholar tree percentage of seedgermination |
CN107683652A (en) * | 2017-09-28 | 2018-02-13 | 南京大花滩生物科技有限公司 | A kind of fast and effective method for improving Chinese scholar tree percentage of seedgermination |
CN107889576A (en) * | 2017-12-07 | 2018-04-10 | 中国科学院华南植物园 | A kind of Scaevola aemula seedling quick efficient breeding method |
CN107926202A (en) * | 2017-12-04 | 2018-04-20 | 广东省农业科学院果树研究所 | A kind of method for promoting half abortive seed of lichee to sprout |
Citations (3)
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CN102870525A (en) * | 2012-09-24 | 2013-01-16 | 南京林业大学 | Method for quickly testing quality of cercis canadensis seeds |
CN103460849A (en) * | 2013-09-24 | 2013-12-25 | 黑龙江省森林植物园 | Method for improving germination rate of Sandersonia aurantiaca seeds |
CN105874965A (en) * | 2016-04-28 | 2016-08-24 | 南京林业大学 | Method for breaking cercis gigantea seed dormancy to promote germination |
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2017
- 2017-01-10 CN CN201710018577.9A patent/CN106879275B/en not_active Expired - Fee Related
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN102870525A (en) * | 2012-09-24 | 2013-01-16 | 南京林业大学 | Method for quickly testing quality of cercis canadensis seeds |
CN103460849A (en) * | 2013-09-24 | 2013-12-25 | 黑龙江省森林植物园 | Method for improving germination rate of Sandersonia aurantiaca seeds |
CN105874965A (en) * | 2016-04-28 | 2016-08-24 | 南京林业大学 | Method for breaking cercis gigantea seed dormancy to promote germination |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107683652A (en) * | 2017-09-28 | 2018-02-13 | 南京大花滩生物科技有限公司 | A kind of fast and effective method for improving Chinese scholar tree percentage of seedgermination |
CN107567756A (en) * | 2017-09-29 | 2018-01-12 | 南京仙草堂生物科技有限公司 | A kind of method for improving Chinese scholar tree percentage of seedgermination |
CN107926202A (en) * | 2017-12-04 | 2018-04-20 | 广东省农业科学院果树研究所 | A kind of method for promoting half abortive seed of lichee to sprout |
CN107926202B (en) * | 2017-12-04 | 2020-10-30 | 广东省农业科学院果树研究所 | Method for promoting germination of litchi semi-aborted seeds |
CN107889576A (en) * | 2017-12-07 | 2018-04-10 | 中国科学院华南植物园 | A kind of Scaevola aemula seedling quick efficient breeding method |
CN107889576B (en) * | 2017-12-07 | 2020-11-10 | 中国科学院华南植物园 | Method for quickly and efficiently breeding pittosporum tobira seedlings |
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