CN107567756A - A kind of method for improving Chinese scholar tree percentage of seedgermination - Google Patents
A kind of method for improving Chinese scholar tree percentage of seedgermination Download PDFInfo
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- CN107567756A CN107567756A CN201710903673.1A CN201710903673A CN107567756A CN 107567756 A CN107567756 A CN 107567756A CN 201710903673 A CN201710903673 A CN 201710903673A CN 107567756 A CN107567756 A CN 107567756A
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Abstract
A kind of method for improving Chinese scholar tree percentage of seedgermination, including by Chinese scholar tree seed with alcohol-pickled, then disinfecting solution of potassium permanganate is used, aseptic water washing is used again, then the moisture of Chinese scholar tree the surface of the seed is aseptically blotted with aseptic filter paper, then cut an openning in the kind skin of the Chinese scholar tree seed with aseptic operation knife and obtain the seed that is open, finally by the opening seed uniform broadcasting into a culture dish；Seed treatment is poured into the culture dish, is soaked under room temperature condition, obtains the Chinese scholar tree seed of imbibition；Then by the Chinese scholar tree seed uniform broadcasting of the imbibition on sand basin face；Obtained seedling length is to two panels true leaf after Chinese scholar tree seed is sprouted, and at a height of 10 15 centimetres, migrates in red soil and is heeled in.The advantages that method of the present invention can apply in the extensive nursery production practices of Chinese scholar tree, have low cost, and technology is simple and practical, and application value is high.
Description
Technical field
The invention belongs to plant seed germination nursery field, more particularly to a kind of fast and effective raising Chinese scholar tree percentage of seedgermination
Method.
Background technology
Chinese scholar tree（Sophora japonica）Also known as family Chinese scholartree, Bai Huai, it is Papilionaceae Sophora deciduous tree.Tree performance is graceful,
Tree crown is circular, and hat is big shady dense, to the pernicious gases such as carbon dioxide, chlorine, hydrogen chloride and the resistance of flue dust, in addition, its life-span
Length, material are hard, strong adaptability, and sophora bud economic value is high, are northern China urban afforestation shade tree and are set with the excellent native soil of material
Kind.
There is Chinese scholar tree seed coat structure legume typically to plant skin feature.Kind skin is formed by outer Ovule Development, and interior ovule exists
Degenerate and disappear before seed development.Kind of skin is followed successively by parenchyma cell layer in irregular shape from inside to outside, iuntercellular has more space
Osteosclereide layer, very thick palisade layer, have angleplied laminate top layer.The parenchyma cell layer of kind endo-endothelial layer is inlayed tightly with endosperm
It is close, and endosperm outermost layer deposits the very high galactomannan gum of viscosity, this be probably cause the kind skin of mature seed with
Endosperm is difficult the reason for separating.Ripe Chinese scholar tree seed, its kind of skin palisade layer is flourishing, and degree of lignification is high, and arrangement is close, top layer
There is fluid-tight wax coat, so as to cause seed water imbibition poor, germination rate is low.
At present in the world have Chinese scholar tree seed sprout nursery document report, but exist germination rate it is unstable, emergence it is not whole
The problem of neat.
The content of the invention
It is an object of the invention to provide a kind of method for improving Chinese scholar tree percentage of seedgermination.
In order to achieve the above objects and other related objects, technical scheme provided by the invention is：One kind improves Chinese scholar tree seed
The method of germination percentage, comprises the following steps：
The first step：By Chinese scholar tree seed with alcohol-pickled, then with disinfecting solution of potassium permanganate, then with aseptic water washing, then exist
The moisture of Chinese scholar tree the surface of the seed is blotted under aseptic condition with aseptic filter paper, then with aseptic operation knife the Chinese scholar tree seed kind
Skin cuts an openning and obtains the seed that is open, finally by the opening seed uniform broadcasting into a culture dish；
Second step：Seed treatment is poured into the culture dish, is soaked under room temperature condition, obtains the Chinese scholar tree seed of imbibition；
Wherein, every liter of seed treatment contains 50-100 mg gibberellin, 0.05-0.1 mg 6- benzyls aminoadenine, 170-
340 mg potassium dihydrogen phosphate；
3rd step：In the river sand in the built-in sand basin of cool canopy, the sand basin filled with water content for 20-25%, then by the imbibition
The uniform broadcasting of Chinese scholar tree seed on sand basin face, then laying depth is 1 ~ 3cm river on the Chinese scholar tree seed of the imbibition
It is husky to cover the Chinese scholar tree seed of the imbibition；
4th step：Obtained seedling length is to two panels true leaf after Chinese scholar tree seed is sprouted, and at a height of 10-15 centimetres, migrates to red soil
In heeled in.
Preferably technical scheme is：In the first step, by Chinese scholar tree seed volume fraction be 60 ~ 85% alcohol-pickled 20 ~
40 seconds, then with the disinfecting solution of potassium permanganate 15 ~ 20 minutes that mass fraction is 0.1 ~ 0.3%, then with aseptic water washing 4 ~ 5 times.
Preferably technical scheme is：In the first step, cut flatly with kind skin of the aseptic operation knife in the Chinese scholar tree seed
The period of the day from 11 p.m. to 1 a.m, avoid embryo position.
Preferably technical scheme is：At a diameter of 15 centimetres of the culture dish, 40 ~ 60 Chinese scholar tree kinds are accommodated per culture dish
Son.
Preferably technical scheme is：In second step, 60 ~ 80 milliliters of seed treatment is poured into the culture dish, in
Soaked 2 ~ 3 days under the conditions of 23 ~ 27 DEG C, obtain the Chinese scholar tree seed of imbibition.
Preferably technical scheme is：In the third step, the shading rate of the cool canopy is more than or equal to 90%.
Preferably technical scheme is：In the third step, long 5 meters, wide 90 centimetres, deep 20 centimetres sand is established in the cool canopy
Pond.
Preferably technical scheme is：In the 4th step, red soil is contained in one 2.7 cun high of white plastic cup.
Because above-mentioned technical proposal is used, the present invention this have the advantage that compared with prior art：
1st, the present invention provides the husky key technology for broadcasting sprouting emergence, seed of the invention in subset for Chinese scholar tree industrial seedling rearing and disappeared
Malicious method, physics and chemical method handle unique and simple material benefit, and application value is high.
2nd, the present invention chooses the seed for maturation of growing in Chinese scholar tree original producton location, is handled by physics and chemical method, will
The germination percentage of Chinese scholar tree seed is stable more than 99.5%, and this method can apply in the extensive nursery production practices of Chinese scholar tree, have
The advantages that low cost, technology is simple and practical, and application value is high.
Embodiment
Embodiments of the present invention are illustrated by particular specific embodiment below, those skilled in the art can be by this implementation
Content disclosed by example understands other advantages and effect of the present invention easily.
Embodiment 1：A kind of method for improving Chinese scholar tree percentage of seedgermination
1st, seed is gathered：The strong state of robust growth, strong stress resistance, disease and insect resistance is chosen from In Nanjing Chinese scholar tree plant cultivation base
Chinese scholartree strain, with the ripening fruits of hand getting collection Chinese scholar tree, is fitted into ventilative good seed collecting bag, naturally dry, rubbed with the hands as seed collecting plant
Go Fruit pod, after exposure seed in the sun, 16 hours, the storage of seeds moisture was controlled in 12%, and full seed is loaded ox-hide
Paper bag, it is placed in 4 DEG C of refrigerators and preserves.
2nd, disinfection seed：Seed is taken out from refrigerator, is 70% in volume fraction after being warming up to room temperature under natural conditions
Alcohol in soak 30 seconds, then with the disinfecting solution of potassium permanganate 20 minutes that mass fraction is 0.2%, aseptic water washing 5 times, in
Blot the moisture of the surface of the seed on superclean bench under aseptic condition with aseptic filter paper, then cut kind of a skin one with aseptic operation knife
Individual osculum, uniform broadcasting is to sprouting in culture dish, per ware（A diameter of 15 centimetres）Place 50, seed.Avoid planting during incision
Embryo position.
3rd, seed treatment soaks：Seed treatment is poured into each culture dish for being covered with seed：60 milliliters, room temperature leaching
Bubble 3 days, obtains the Chinese scholar tree seed of imbibition.Seed treatment composition is gibberellin（GA3）50 mg/L, 6- benzyl aminoadenines（6-
BA）0.05 mg/L, the mg/L of potassium dihydrogen phosphate 170.Actual to measure, under the room temperature condition, actual temperature is 25 DEG C.
4th, the husky seed for broadcasting imbibition：It is that long 5 meters, wide 90 centimetres, deep 20 centimetres sand is established in 90% cool canopy in shading rate
Pond, sand basin are filled to a height of 15 centimetres of clean river sand, 25% water content, and uniform broadcasting seed is on sand basin face, then in kind
Thickness 2cm river sands are spread on sub- face and cover seed.Germination percentage is counted after 7d, the Chinese scholar tree percentage of seedgermination handled through this method is up to
More than 99.5%.
5th, upper cup is heeled in：Using 2.7 cun of white plastic cups, matrix is red soil, high when Chinese scholar tree grows to two panels true leaf
At 10-15 centimetres, seedling is moved into cup from sand basin and heeled in, carries out normal water and fertilizer management.
Embodiment 2：A kind of method for improving Chinese scholar tree percentage of seedgermination
1st, seed is gathered：The strong state of robust growth, strong stress resistance, disease and insect resistance is chosen from In Nanjing Chinese scholar tree plant cultivation base
Chinese scholartree strain, with the ripening fruits of hand getting collection Chinese scholar tree, is fitted into ventilative good seed collecting bag, naturally dry, rubbed with the hands as seed collecting plant
Go Fruit pod, after exposure seed 24 hours in the sun, the storage of seeds moisture controlled in 10%, and full seed is loaded ox-hide
Paper bag, it is placed in 4 DEG C of refrigerators and preserves.
2nd, disinfection seed：Seed is taken out from refrigerator after being warming up to room temperature under natural conditions, be 80% in volume fraction
Soaked 20 seconds in alcohol, then with the disinfecting solution of potassium permanganate 20 minutes that mass fraction is 0.15%, aseptic water washing 4 times, Yu Chao
Blot the moisture of the surface of the seed on net workbench under aseptic condition with aseptic filter paper, then cut kind of a skin with aseptic operation knife（Keep away
Open embryo position）One osculum, uniform broadcasting is to sprouting in culture dish, per ware（A diameter of 15 centimetres）Place 50, seed.
3rd, seed treatment soaks：Seed treatment is poured into each culture dish for being covered with seed：80 milliliters, room temperature
(25 ± 2) DEG C immersion 2 days, obtains the Chinese scholar tree seed of imbibition.Seed treatment composition is 0.1 mg/ of mg/L, 6-BA of GA3 100
L, 340 mg/L potassium dihydrogen phosphates.Actual to measure, under the room temperature condition, actual temperature is 26 DEG C
4th, the husky seed for broadcasting imbibition：It is that long 5 meters, wide 90 centimetres, deep 20 centimetres sand basin is established in 90% cool canopy in shading rate, it is husky
Pond is filled to a height of 15 centimetres of clean river sand, 25% water content, and uniform broadcasting seed is on sand basin face, then on seed face
Spread thickness 2cm river sands and cover seed.Germination percentage is counted after 7d, the Chinese scholar tree percentage of seedgermination handled through this method is up to 99.8%
More than.
5th, upper cup is heeled in：Using 2.7 cun of white plastic cups, matrix is red soil, high when Chinese scholar tree grows to two panels true leaf
At 10-15 centimetres, seedling is moved into cup from sand basin and heeled in, carries out normal water and fertilizer management.
Embodiment 3：A kind of method for improving Chinese scholar tree percentage of seedgermination
A kind of method for improving Chinese scholar tree percentage of seedgermination, comprises the following steps：
The first step：By Chinese scholar tree seed with alcohol-pickled, then with disinfecting solution of potassium permanganate, then with aseptic water washing, then exist
The moisture of Chinese scholar tree the surface of the seed is blotted under aseptic condition with aseptic filter paper, then with aseptic operation knife the Chinese scholar tree seed kind
Skin cuts an openning and obtains the seed that is open, finally by the opening seed uniform broadcasting into a culture dish；
Second step：Seed treatment is poured into the culture dish, is soaked under room temperature condition, obtains the Chinese scholar tree seed of imbibition；
Wherein, every liter of seed treatment contains 80 mg gibberellin, 0.08 mg 6- benzyls aminoadenine, 330 mg phosphoric acid
Potassium dihydrogen；
3rd step：In being filled with the river sand that water content is 22% in the built-in sand basin of cool canopy, the sand basin, then by the imbibition
The uniform broadcasting of Chinese scholar tree seed on sand basin face, river sand that then laying depth is 1cm on the Chinese scholar tree seed of the imbibition with
Cover the Chinese scholar tree seed of the imbibition；
4th step：Obtained seedling length is to two panels true leaf after Chinese scholar tree seed is sprouted, and at a height of 14 centimetres, migrates in red soil
Row is heeled in.
Preferred embodiment is：In the first step, by alcohol-pickled 28 seconds that Chinese scholar tree seed volume fraction is 65%,
Then with the disinfecting solution of potassium permanganate 18 minutes that mass fraction is 0.2%, then with aseptic water washing 4 times.
Preferred embodiment is：In the first step, cut flatly with kind skin of the aseptic operation knife in the Chinese scholar tree seed
The period of the day from 11 p.m. to 1 a.m, avoid embryo position.
Preferred embodiment is：At a diameter of 15 centimetres of the culture dish, 45 Chinese scholar tree seeds are accommodated per culture dish.
Preferred embodiment is：In second step, 63 milliliters of seed treatment is poured into the culture dish, in 24 DEG C
Under the conditions of soak 2.5 days, obtain the Chinese scholar tree seed of imbibition.
Preferred embodiment is：In the third step, the shading rate of the cool canopy is 92%.
Preferred embodiment is：In the third step, long 5 meters, wide 90 centimetres, deep 20 centimetres sand is established in the cool canopy
Pond.
Preferably technical scheme is：In the 4th step, red soil is contained in one 2.7 cun high of white plastic cup.
Embodiment 4：A kind of method for improving Chinese scholar tree percentage of seedgermination
A kind of method for improving Chinese scholar tree percentage of seedgermination, comprises the following steps：
The first step：By Chinese scholar tree seed with alcohol-pickled, then with disinfecting solution of potassium permanganate, then with aseptic water washing, then exist
The moisture of Chinese scholar tree the surface of the seed is blotted under aseptic condition with aseptic filter paper, then with aseptic operation knife the Chinese scholar tree seed kind
Skin cuts an openning and obtains the seed that is open, finally by the opening seed uniform broadcasting into a culture dish；
Second step：Seed treatment is poured into the culture dish, is soaked under room temperature condition, obtains the Chinese scholar tree seed of imbibition；
Wherein, every liter of seed treatment contains 90 mg gibberellin, 0.05 mg 6- benzyls aminoadenine, 170mg phosphoric acid
Potassium dihydrogen；
3rd step：In being filled with the river sand that water content is 25% in the built-in sand basin of cool canopy, the sand basin, then by the imbibition
The uniform broadcasting of Chinese scholar tree seed on sand basin face, river sand that then laying depth is 3cm on the Chinese scholar tree seed of the imbibition with
Cover the Chinese scholar tree seed of the imbibition；
4th step：Obtained seedling length is to two panels true leaf after Chinese scholar tree seed is sprouted, and at a height of 15 centimetres, migrates in red soil
Row is heeled in.
Preferred embodiment is：In the first step, by alcohol-pickled 20 seconds that Chinese scholar tree seed volume fraction is 85%,
Then with the disinfecting solution of potassium permanganate 15 minutes that mass fraction is 0.1%, then with aseptic water washing 5 times.
Preferred embodiment is：In the first step, cut flatly with kind skin of the aseptic operation knife in the Chinese scholar tree seed
The period of the day from 11 p.m. to 1 a.m, avoid embryo position.
Preferred embodiment is：At a diameter of 15 centimetres of the culture dish, 60 Chinese scholar tree seeds are accommodated per culture dish.
Preferred embodiment is：In second step, 80 milliliters of seed treatment is poured into the culture dish, in 27 DEG C
Under the conditions of soak 2 days, obtain the Chinese scholar tree seed of imbibition.
Preferred embodiment is：In the third step, the shading rate of the cool canopy is 91%.
Preferred embodiment is：In the third step, long 5 meters, wide 90 centimetres, deep 20 centimetres sand is established in the cool canopy
Pond.
Preferred embodiment is：In the 4th step, red soil is contained in one 2.7 cun high of white plastic cup.
Embodiment 5：A kind of method for improving Chinese scholar tree percentage of seedgermination
A kind of method for improving Chinese scholar tree percentage of seedgermination, comprises the following steps：
The first step：By Chinese scholar tree seed with alcohol-pickled, then with disinfecting solution of potassium permanganate, then with aseptic water washing, then exist
The moisture of Chinese scholar tree the surface of the seed is blotted under aseptic condition with aseptic filter paper, then with aseptic operation knife the Chinese scholar tree seed kind
Skin cuts an openning and obtains the seed that is open, finally by the opening seed uniform broadcasting into a culture dish；
Second step：Seed treatment is poured into the culture dish, is soaked under room temperature condition, obtains the Chinese scholar tree seed of imbibition；
Wherein, every liter of seed treatment contains 50 mg gibberellin, 0.05mg 6- benzyls aminoadenine, 170 mg phosphoric acid
Potassium dihydrogen；
3rd step：In being filled with the river sand that water content is 20% in the built-in sand basin of cool canopy, the sand basin, then by the imbibition
The uniform broadcasting of Chinese scholar tree seed is on sand basin face, and river sand that then laying depth is 1m on the Chinese scholar tree seed of the imbibition is to cover
State the Chinese scholar tree seed of imbibition in residence；
4th step：Obtained seedling length is to two panels true leaf after Chinese scholar tree seed is sprouted, and at a height of 10 centimetres, migrates in red soil
Row is heeled in.
Preferred embodiment is：In the first step, by alcohol-pickled 20 seconds that Chinese scholar tree seed volume fraction is 60%,
Then with the disinfecting solution of potassium permanganate 15 minutes that mass fraction is 0.1%, then with aseptic water washing 4 times.
Preferred embodiment is：In the first step, cut flatly with kind skin of the aseptic operation knife in the Chinese scholar tree seed
The period of the day from 11 p.m. to 1 a.m, avoid embryo position.
Preferred embodiment is：At a diameter of 15 centimetres of the culture dish, 40 Chinese scholar tree seeds are accommodated per culture dish.
Preferred embodiment is：In second step, 60 milliliters of seed treatment is poured into the culture dish, in 23 DEG C
Under the conditions of soak 2 days, obtain the Chinese scholar tree seed of imbibition.
Preferred embodiment is：In the third step, the shading rate of the cool canopy is 93%.
Preferred embodiment is：In the third step, long 5 meters, wide 90 centimetres, deep 20 centimetres sand is established in the cool canopy
Pond.
Preferred embodiment is：In the 4th step, red soil is contained in one 2.7 cun high of white plastic cup.
As described above is only to explain the preferred embodiments of the invention, is not intended to tool to do any shape to the present invention
Limitation in formula, therefore all have any modification or change for making the relevant present invention under identical spirit, it should all wrap
Include the invention is intended to the category protected.
Claims (8)
- A kind of 1. method for improving Chinese scholar tree percentage of seedgermination, it is characterised in that：Comprise the following steps：The first step：By Chinese scholar tree seed with alcohol-pickled, then with disinfecting solution of potassium permanganate, then with aseptic water washing, then exist The moisture of Chinese scholar tree the surface of the seed is blotted under aseptic condition with aseptic filter paper, then with aseptic operation knife the Chinese scholar tree seed kind Skin cuts an openning and obtains the seed that is open, finally by the opening seed uniform broadcasting into a culture dish；Second step：Seed treatment is poured into the culture dish, is soaked under room temperature condition, obtains the Chinese scholar tree seed of imbibition； Wherein, every liter of seed treatment contains 50-100 mg gibberellin, 0.05-0.1 mg 6- benzyls aminoadenine, 170- 340 mg potassium dihydrogen phosphate；3rd step：In the river sand in the built-in sand basin of cool canopy, the sand basin filled with water content for 20-25%, then by the imbibition The uniform broadcasting of Chinese scholar tree seed on sand basin face, then laying depth is 1 ~ 3cm river on the Chinese scholar tree seed of the imbibition It is husky to cover the Chinese scholar tree seed of the imbibition；4th step：Obtained seedling length is to two panels true leaf after Chinese scholar tree seed is sprouted, and at a height of 10-15 centimetres, migrates to red soil In heeled in.
- 2. the method according to claim 1 for improving Chinese scholar tree percentage of seedgermination, it is characterised in that：In the first step, by state Chinese scholartree seed volume fraction is alcohol-pickled 20 ~ 40 seconds of 60 ~ 85%, then with the potassium permanganate that mass fraction is 0.1 ~ 0.3% Solution disinfection 15 ~ 20 minutes, then with aseptic water washing 4 ~ 5 times.
- 3. the method according to claim 1 for improving Chinese scholar tree percentage of seedgermination, it is characterised in that：In the first step, with nothing Bacterium scalpel cuts a bite period of the day from 11 p.m. to 1 a.m in the kind skin of the Chinese scholar tree seed, avoids embryo position.
- 4. the method according to claim 1 for improving Chinese scholar tree percentage of seedgermination, it is characterised in that：The diameter of the culture dish For 15 centimetres when, per culture dish accommodate 40 ~ 60 Chinese scholar tree seeds.
- 5. the method according to claim 1 for improving Chinese scholar tree percentage of seedgermination, it is characterised in that：In second step, to institute State and 60 ~ 80 milliliters of seed treatment is poured into culture dish, soaked 2 ~ 3 days under the conditions of 23 ~ 27 DEG C, obtain the Chinese scholar tree kind of imbibition Son.
- 6. the method according to claim 1 for improving Chinese scholar tree percentage of seedgermination, it is characterised in that：In the third step, it is described The shading rate of cool canopy is more than or equal to 90%.
- 7. the method according to claim 1 for improving Chinese scholar tree percentage of seedgermination, it is characterised in that：In the third step, it is described Long 5 meters, wide 90 centimetres, deep 20 centimetres sand basin is established in cool canopy.
- 8. the method according to claim 1 for improving Chinese scholar tree percentage of seedgermination, it is characterised in that：In the 4th step, red soil Soil is contained in one 2.7 cun high of white plastic cup.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106879275A (en) * | 2017-01-10 | 2017-06-23 | 中国科学院华南植物园 | A kind of quick method for effectively improving Hainan Chinese scholartree percentage of seedgermination |
| CN107683652A (en) * | 2017-09-28 | 2018-02-13 | 南京大花滩生物科技有限公司 | A kind of fast and effective method for improving Chinese scholar tree percentage of seedgermination |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106879275A (en) * | 2017-01-10 | 2017-06-23 | 中国科学院华南植物园 | A kind of quick method for effectively improving Hainan Chinese scholartree percentage of seedgermination |
| CN107683652A (en) * | 2017-09-28 | 2018-02-13 | 南京大花滩生物科技有限公司 | A kind of fast and effective method for improving Chinese scholar tree percentage of seedgermination |
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Application publication date: 20180112 |