CN105874965A - Method for breaking cercis gigantea seed dormancy to promote germination - Google Patents
Method for breaking cercis gigantea seed dormancy to promote germination Download PDFInfo
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- CN105874965A CN105874965A CN201610274069.2A CN201610274069A CN105874965A CN 105874965 A CN105874965 A CN 105874965A CN 201610274069 A CN201610274069 A CN 201610274069A CN 105874965 A CN105874965 A CN 105874965A
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- cercis
- seed
- gigantea
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- seed dormancy
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a method for breaking cercis gigantea seed dormancy to promote germination and belongs to the technical field of seed treatment. The method includes the following steps that after shells of mature cercis gigantea fruits are removed, clean and full seeds are selected, treatment is performed for 15-20 minutes with 98% concentrated sulfuric acid, the solution is constantly stirred by using a glass rod during treatment, the treated seeds are flushed with flowing water for 12 hours after treatment, then treatment is performed with 500 mg mL<-1> gibberellin for 12 hours, the seeds are put in laminated low-temperature sand for storage for 45 days, then the cercis gigantea seed dormancy can be broken, and the germination rate can be 94% or above. The method can not only effectively break the cercis gigantea seed dormancy but also remarkably increase the germination rate of the cercis gigantea seeds, achieves the purposes of promoting rapid germination of the cercis gigantea seeds, expanding cercis gigantea breeding and meeting the garden greening demand, has a very good practical application value and can produce good economic benefits and social benefits.
Description
Technical field
Can select during the present invention relates to preprocess method that seed field sowing can use or seed testing
Preprocess method, is specifically related to a kind of method releasing huge cercis seed dormancy stratification.
Background technology
Huge cercis (Cercis gigante), pulse family, a kind of Redbud, China is peculiar, and the life-span is long, be existing seldom
Indigenous tree, NATURAL DISTRIBUTION in Zhejiang, Anhui, Henan, Shaanxi, the major part mountain area such as Sichuan.These seeds are fallen leaves
Arbor or megaphanerophyte, the diameter of a cross-section of a tree trunk 1.3 meters above the ground up to 40 centimetres, up to 15 meters.Huge cercis leaf alternate, Quan Yuan, heart or subcircular,
Branch softness is sagging, dense elegant.Calyx aubergine, the purple butterfly of likeness in form, first spend posterior lobe.Whenever early spring, branch is covered with purple
Flower, such as Yun Ruxia, brocade is bright reflects sky.Summer, leaf green pods was red；Autumn coloured silk leaf such as rosy clouds；After falling leaves winter, pod still hangs branch
Head opens up to the greatest extent the strength power of life.It it is the indigenous tree in afforestation with broad prospect of application.It is suitable for greenery patches isolated planting, group planting,
Or with other trees mixed plantings, it is possible to making garden tree or shade tree and coordinate plantation with evergreen tree, the green and the end setting off each other for spring flower autumnal scenery, feelings
Scape is outstanding.
Huge cercis generally uses sowing and breeding, after seed is often soaked 24h in 60 DEG C to 80 DEG C water by production, it is husky
Sow after hiding accelerating germination, but germination percentage is only 40%.Research shows that this seed has obvious physics and physiology combinational dormancy is practised
Property, this give seed testing, field sowing work bring a lot of inconvenience, the dormancy therefore releasing huge cercis seed removes
Outside the saturating property increasing seed coat that to take appropriate measures, in addition it is also necessary to release physiological dormancy produced by endosperm.So for
The seed dormant reason of huge cercis, seeks simplicity, the efficient method field sprouting to this seed of acceleration releasing its dormancy
Tool is of great significance.
Summary of the invention
The purpose of invention: for the seed dormant reason of huge cercis, it is an object of the invention to provide a kind of easy, efficient
The method releasing huge cercis seed dormancy stratification, this to accelerate seed in field rapidly, fitly sprout have very
Important meaning.
Technical scheme: in order to realize foregoing invention purpose, the technical solution used in the present invention is as follows:
A kind of method releasing huge cercis seed dormancy stratification, after being removed by huge cercis maturation shell, selects clean full
Full seed, first with 98% dense sulfuric acid treatment 10～20min, rinses with flowing water after process, then is 150～500 by concentration
mg·mL-1Gibberellin treatment, is subsequently placed in 2～5 DEG C of sand at low temperature and hides lamination.
Described husky hide lamination, substrate be the ratio of river sand, seed and substrate be 1:3, lamination temperature is 2～5 DEG C, layer
The long-pending time is often to stir seed during 45d, lamination, is beneficial to ventilation, and keeps the moistening of river sand.
Described concentrated sulphuric acid concentration is 98%, and the process time is 15～20min, is stirred continuously solution with Glass rod during process.
The described method releasing huge cercis seed dormancy stratification, rinses 12h with flowing water after dense sulfuric acid treatment, then uses
Concentration is 200～500mg mL-1Gibberellins solution processes 12h.
The described method releasing huge cercis seed dormancy stratification, first with 98% dense sulfuric acid treatment 15～20min, place
Rinse 12h with flowing water after reason, then be 500mg mL by concentration-1Gibberellin treatment 12h, is subsequently placed in 2～5 DEG C of sand at low temperature
Hide lamination 45d.
Beneficial effect: compared with prior art, the method releasing huge cercis seed dormancy stratification of the present invention, use
First concentrated sulphuric acid acid etching, rear gibberellin treatment again after cool stratification, the germination percentage of huge cercis seed up to more than 94%, and
The percentage of seedgermination hiding accelerating germination merely through hot-water soak sand is only 40%, the unused gibberellins of dense sulfuric acid treatment and cool stratification
Germination percentage is only 4%, hot-water soak (50～80 DEG C), gibberellins (150～500mg mL-1) husky after process hide lamination
The germination percentage of (45～60d) is up to 67%.Therefore, the preprocess method of this invention, simple to operate, quick, can be
Complete seed dormant releasing in short period and promote it to germinate, for before huge cercis seeding and seedling raising seed pretreatment and
The quality inspection of indoor seed provides the technological means of high efficient and reliable, has good actual application value, it is possible to produce
Preferably economic benefit and social benefit.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further illustrated.The following stated is only the present invention and preferably implements
Example, is used only for describing the present invention, it is impossible to be interpreted as limiting the scope of the present invention.It should be pointed out that, it is all at this
Any amendment, equivalent and the improvement etc. made within the spirit of invention and principle, should be included in the protection of the present invention
Within the scope of.
Embodiment 1
In breeding, in order to enable huge cercis seed to sprout smoothly, need to release physics dormancy and the physiological dormancy of seed.
Release the physics dormancy of seed, it is simply that release fine and close seed coat to seed water suction and ventilative impact.The most every can make kind
The method that transdermally is improved can be selected.But actually use in there will be undesirable effect, the present embodiment with
Hot water treatment and the method for dense sulfuric acid treatment, to how releasing huge cercis seed dormancy illustrate.
Respectively with dense sulfuric acid treatment 10min (1. group), 15min (2. group), 20min (3. group), 25min (4. group),
30min (5. group), period is stirred continuously solution with Glass rod, after processing the corresponding time, is respectively placed under flowing water flushing
48h.Each process 3 repetition, each repetition 100.After being disposed, carry out germination test, seed is placed in
In moistening sand, 25 DEG C of cultivation temperature, illumination every day 12h, intensity of illumination 1600lx, sand in period holding germination box
Son is moistening.
Respectively by 50 DEG C (6. groups), 60 DEG C (7. group), 70 DEG C (8. group), 80 DEG C (9. group), 90 DEG C (10. group) heat
Water soaking huge cercis seed 48h, 3 repetitions, each repetition 100, after being disposed, carry out germination test, will
Seed is placed in moistening sand, 25 DEG C of cultivation temperature, illumination every day 12h, intensity of illumination 1600lx, and period keeps sending out
In bud box, sand moistens.
Result after table 1 huge cercis seed concentrated sulphuric acid, hot water treatment
| Process | There is vitality number | Number without vitality | Germinative number | Mouldy number |
| ① | 48 | 2 | 0 | 0 |
| ② | 49 | 1 | 0 | 0 |
| ③ | 45 | 5 | 0 | 0 |
| ④ | 46 | 2 | 0 | 2 |
| ⑤ | 43 | 1 | 2 | 4 |
| ⑥ | 47 | 3 | 0 | 0 |
| ⑦ | 50 | 0 | 0 | 0 |
| ⑧ | 47 | 3 | 0 | 0 |
| ⑨ | 45 | 4 | 0 | 1 |
| ⑩ | 42 | 4 | 0 | 4 |
Dense sulfuric acid treatment, hot-water soak huge cercis seed all can not release the dormancy of huge cercis seed as can be seen from Table 1,
Only have 2 seed germinations.
Embodiment 2
In breeding, in order to enable huge cercis seed to sprout smoothly, need to release physics dormancy and the physiological dormancy of seed.
Release the physics dormancy of seed, it is simply that release fine and close seed coat to seed water suction and ventilative impact.The most every can make kind
The method that transdermally is improved can be selected.But there will be undesirable effect in actually using, the present embodiment is first
With Soaking in hot water release physics dormancy, after with gibberellins soak again sand at low temperature hide lamination method release physiological dormancy, to such as
What releases huge cercis seed dormancy illustrates.
Respectively with 50 DEG C, 70 DEG C, after the Soaking in hot water 12h of 80 DEG C, then be 0mg mL by concentration-1、150mg·mL-1、
300mg·mL-1、500mg·mL-1Gibberellins solution soaks huge cercis seed 12h, often processes 3 repetitions, each repetition
200 seeds.It is disposed and is placed under 2～5 DEG C of low temperature husky Tibetan lamination 45d.After being disposed, carry out germination test,
Being placed in by seed in moistening sand, 25 DEG C of cultivation temperature, illumination every day 12h, intensity of illumination 1600lx, period keeps
In germination box, sand moistens.
Germination percentage after hypothermia grait hides 45d after the process of table 2 Soaking in hot water, gibberellin treatment
As can be seen from Table 2, through 50 DEG C, 70 DEG C, 80 DEG C of Soaking in hot water process, hypothermia grait hides 45d after gibberellin treatment
After only 80 DEG C Soaking in hot water 12h, 500mg mL of germination percentage of each combination-1Gibberellins solution after the reaching of lamination 45d
53.33%, remaining processes germination percentage and is below 50%.After Soaking in hot water is described, gibberellin treatment can improve to a certain extent
The germination rate of huge cercis seed, and improve along with the raising of hot water temperature.But after the seed for sprouting is dissected
Finding, rotting occurs in the seed that part is not sprouted, and wherein the rotting rate with the seed of 70 DEG C of hot-water soaks is the highest.This may
When being due to Soaking in hot water, Some seeds is scalded dead caused.
Embodiment 3
In breeding, in order to enable huge cercis seed to sprout smoothly, need to release physics dormancy and the physiological dormancy of seed.
Release the physics dormancy of seed, it is simply that release fine and close seed coat to seed water suction and ventilative impact.The most every can make kind
The method that transdermally is improved can be selected.But there will be undesirable effect in actually using, the present embodiment is first
With 98% concentrated sulphuric acid acid etching release physics dormancy, after with gibberellins soak again sand at low temperature hide lamination method release physiology stop
Sleeping, to how releasing huge cercis seed dormancy illustrating.
Respectively with 98% dense sulfuric acid treatment 5min, 10min, 15min, 20min, it is stirred continuously with Glass rod during process
Solution, rinses 12h with flowing water after process, then soaks seed after 12h respectively with the gibberellins of variable concentrations respectively, and sand at low temperature is hidden
45d.After being disposed, carry out germination test, seed is placed in moistening sand, 25 DEG C of cultivation temperature, every daylight
According to 12h, intensity of illumination 1600lx, in period holding germination box, sand moistens.Take 30 seeds respectively and compare its germination percentage.
After table 3 98% concentrated sulphuric acid acid etching, gibberellin treatment, hypothermia grait hides the result of lamination 45d
As can be seen from Table 3, sending out of each process after hypothermia grait hides 45d after 98% concentrated sulphuric acid acid etching, gibberellin treatment
Bud rate is all significantly increased, wherein 98% concentrated sulphuric acid acid etching 15～20min, uses 500mg mL afterwards-1Gibberellin treatment warp again
The germination percentage of the process that sand at low temperature hides lamination 45d reaches more than 94%.
Claims (5)
1. the method releasing huge cercis seed dormancy stratification, it is characterised in that: after being removed by huge cercis maturation shell, select the fullest seed, first with 98% dense sulfuric acid treatment 10 ~ 20min, rinse with flowing water after process, then be 150 ~ 500 mg mL by concentration-1Gibberellin treatment, is subsequently placed in 2 ~ 5 DEG C of sand at low temperature and hides lamination.
The method of releasing the most according to claim 1 huge cercis seed dormancy stratification, it is characterized in that: described husky Tibetan lamination, substrate is river sand, the ratio of seed and substrate is 1:3, lamination temperature is 2 ~ 5 DEG C, and Stratification time is often to stir seed during 45d, lamination, it is beneficial to ventilation, and keeps the moistening of river sand.
The method of releasing the most according to claim 1 huge cercis seed dormancy stratification, it is characterised in that: described concentrated sulphuric acid concentration is 98%, and the process time is 15 ~ 20min, is stirred continuously solution with Glass rod during process.
The method of releasing the most according to claim 1 huge cercis seed dormancy stratification, it is characterised in that: rinse 12h with flowing water after dense sulfuric acid treatment, then be 200 ~ 500 by concentration
mg·mL-1Gibberellins solution processes 12h.
The method of releasing the most according to claim 1 huge cercis seed dormancy stratification, it is characterised in that: first with 98% dense sulfuric acid treatment 15 ~ 20min, rinse 12h with flowing water after process, then be 500 mg mL by concentration-1Gibberellin treatment 12h, is subsequently placed in 2 ~ 5 DEG C of sand at low temperature and hides lamination 45d.
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Cited By (5)
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| CN106358913A (en) * | 2016-08-27 | 2017-02-01 | 柳州市绿化工程处 | Method for introducing and domesticating bauhinia galpinii |
| CN106879275A (en) * | 2017-01-10 | 2017-06-23 | 中国科学院华南植物园 | A kind of quick method for effectively improving Hainan Chinese scholartree percentage of seedgermination |
| CN107114030A (en) * | 2017-04-10 | 2017-09-01 | 周兴虎 | A kind of storage method of Piao Shu seeds |
| CN108633379A (en) * | 2018-06-14 | 2018-10-12 | 长江大学 | A method of abolishing huge cercis seed dormancy |
| CN113748775A (en) * | 2021-08-05 | 2021-12-07 | 广东省农业科学院作物研究所 | Method for promoting germination of murraya paniculata seeds |
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| CN102308689A (en) * | 2011-06-27 | 2012-01-11 | 苏芹 | Technique for accelerating germination of flower seeds |
| CN102823360A (en) * | 2012-09-24 | 2012-12-19 | 南京林业大学 | Method for releasing dormancy of Canadian redbud seeds |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106358913A (en) * | 2016-08-27 | 2017-02-01 | 柳州市绿化工程处 | Method for introducing and domesticating bauhinia galpinii |
| CN106879275A (en) * | 2017-01-10 | 2017-06-23 | 中国科学院华南植物园 | A kind of quick method for effectively improving Hainan Chinese scholartree percentage of seedgermination |
| CN107114030A (en) * | 2017-04-10 | 2017-09-01 | 周兴虎 | A kind of storage method of Piao Shu seeds |
| CN108633379A (en) * | 2018-06-14 | 2018-10-12 | 长江大学 | A method of abolishing huge cercis seed dormancy |
| CN113748775A (en) * | 2021-08-05 | 2021-12-07 | 广东省农业科学院作物研究所 | Method for promoting germination of murraya paniculata seeds |
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| CN105874965B (en) | 2018-12-07 |
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Address after: Longpan road Xuanwu District of Nanjing city of Jiangsu Province, No. 159 210037 Co-patentee after: Jinggu Environmental Construction Co.,Ltd. Patentee after: NANJING FORESTRY University Address before: Longpan road Xuanwu District of Nanjing city of Jiangsu Province, No. 159 210037 Co-patentee before: Jiangsu Jinggu Environment Construction Co.,Ltd. Patentee before: NANJING FORESTRY University |