CN106748201A - A kind of preparation method of Pleurotus citrinopileatus culture medium - Google Patents
A kind of preparation method of Pleurotus citrinopileatus culture medium Download PDFInfo
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- 241000392443 Pleurotus citrinopileatus Species 0.000 title claims abstract description 47
- 239000001963 growth medium Substances 0.000 title claims abstract description 23
- 238000002360 preparation method Methods 0.000 title claims abstract description 23
- 238000000855 fermentation Methods 0.000 claims abstract description 18
- 230000004151 fermentation Effects 0.000 claims abstract description 18
- 241000124033 Salix Species 0.000 claims abstract description 11
- 229920000742 Cotton Polymers 0.000 claims abstract description 9
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims abstract description 8
- 229910052761 rare earth metal Inorganic materials 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000002023 wood Substances 0.000 claims abstract description 8
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000004202 carbamide Substances 0.000 claims abstract description 6
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims abstract description 5
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims abstract description 5
- 235000011130 ammonium sulphate Nutrition 0.000 claims abstract description 5
- 235000008733 Citrus aurantifolia Nutrition 0.000 claims abstract description 4
- 235000011941 Tilia x europaea Nutrition 0.000 claims abstract description 4
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 claims abstract description 4
- 229910000389 calcium phosphate Inorganic materials 0.000 claims abstract description 4
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 claims abstract description 4
- 239000004571 lime Substances 0.000 claims abstract description 4
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims abstract description 4
- 235000019341 magnesium sulphate Nutrition 0.000 claims abstract description 4
- 238000002156 mixing Methods 0.000 claims abstract description 4
- 235000019691 monocalcium phosphate Nutrition 0.000 claims abstract description 4
- 229960001471 sodium selenite Drugs 0.000 claims abstract description 4
- 239000011781 sodium selenite Substances 0.000 claims abstract description 4
- 235000015921 sodium selenite Nutrition 0.000 claims abstract description 4
- 239000002245 particle Substances 0.000 claims description 8
- 238000009423 ventilation Methods 0.000 claims description 8
- 239000000463 material Substances 0.000 claims description 5
- 235000019738 Limestone Nutrition 0.000 claims description 3
- HSJPMRKMPBAUAU-UHFFFAOYSA-N cerium(3+);trinitrate Chemical compound [Ce+3].[O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O HSJPMRKMPBAUAU-UHFFFAOYSA-N 0.000 claims description 3
- 239000002361 compost Substances 0.000 claims description 3
- GJKFIJKSBFYMQK-UHFFFAOYSA-N lanthanum(3+);trinitrate;hexahydrate Chemical compound O.O.O.O.O.O.[La+3].[O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O GJKFIJKSBFYMQK-UHFFFAOYSA-N 0.000 claims description 3
- 239000006028 limestone Substances 0.000 claims description 3
- 230000002269 spontaneous effect Effects 0.000 claims description 3
- 239000010902 straw Substances 0.000 claims description 3
- 241000219000 Populus Species 0.000 claims 1
- 150000002910 rare earth metals Chemical group 0.000 claims 1
- 150000001413 amino acids Chemical class 0.000 abstract description 13
- 235000016709 nutrition Nutrition 0.000 abstract description 5
- 230000035764 nutrition Effects 0.000 abstract description 5
- 235000001014 amino acid Nutrition 0.000 description 12
- 229940024606 amino acid Drugs 0.000 description 12
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 8
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 244000103635 Lyophyllum ulmarium Species 0.000 description 5
- 235000015934 Lyophyllum ulmarium Nutrition 0.000 description 5
- 235000018102 proteins Nutrition 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 240000001462 Pleurotus ostreatus Species 0.000 description 3
- 235000001603 Pleurotus ostreatus Nutrition 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 3
- 239000010931 gold Substances 0.000 description 3
- 229910052737 gold Inorganic materials 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- GHOKWGTUZJEAQD-ZETCQYMHSA-N (D)-(+)-Pantothenic acid Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-ZETCQYMHSA-N 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical group [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 235000010755 mineral Nutrition 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 150000003722 vitamin derivatives Chemical class 0.000 description 2
- 241000222485 Agaricales Species 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- GHOKWGTUZJEAQD-UHFFFAOYSA-N Chick antidermatitis factor Natural products OCC(C)(C)C(O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-UHFFFAOYSA-N 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 241001148715 Lamarckia aurea Species 0.000 description 1
- 208000008589 Obesity Diseases 0.000 description 1
- 241001492261 Pleurotaceae Species 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 240000006794 Volvariella volvacea Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 159000000007 calcium salts Chemical class 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 238000010411 cooking Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- PXEDJBXQKAGXNJ-QTNFYWBSSA-L disodium L-glutamate Chemical compound [Na+].[Na+].[O-]C(=O)[C@@H](N)CCC([O-])=O PXEDJBXQKAGXNJ-QTNFYWBSSA-L 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 208000001848 dysentery Diseases 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 239000002360 explosive Substances 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 235000012041 food component Nutrition 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 159000000003 magnesium salts Chemical class 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 238000011177 media preparation Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000011785 micronutrient Substances 0.000 description 1
- 235000013369 micronutrients Nutrition 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 235000013923 monosodium glutamate Nutrition 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 235000020824 obesity Nutrition 0.000 description 1
- 235000019161 pantothenic acid Nutrition 0.000 description 1
- 239000011713 pantothenic acid Substances 0.000 description 1
- 229940055726 pantothenic acid Drugs 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 150000003342 selenium Chemical class 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 229940073490 sodium glutamate Drugs 0.000 description 1
- 235000013547 stew Nutrition 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 235000016804 zinc Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fertilizers (AREA)
Abstract
The invention provides a kind of preparation method of Pleurotus citrinopileatus culture medium, comprise the following steps:1) after, mixing 25 35 parts of 20 30 parts of willow wood chip, 30 40 parts of cotton stalk and corncob, 50 100 parts of water is added, builds heap fermentation;2) after, fermenting, 0.001 0.002 parts of rare earth element solution is sprinkled, continues to ferment;3) 10 30 parts of 23 parts of urea, 0.5 1 parts of ammonium sulfate, 0.02 0.05 parts of magnesium sulfate, 0.02 0.2 parts of calcium superphosphate, 0.02 0.1 parts of sodium selenite and water, are subsequently adding, heap fermentation is built;4) and then, add 0.2 3 parts of lime, sugar 0.3 0.8 parts, build heap fermentation, obtain Pleurotus citrinopileatus culture medium.Compared with prior art, the cultivation base comprehensive nutrition that the present invention is provided, is conducive to the growth of Pleurotus citrinopileatus, and, it is possible to increase the amino acid content of Pleurotus citrinopileatus, improves its health care and medical value.
Description
Technical field
The invention belongs to mushroom culture field, and in particular to a kind of preparation method of Pleurotus citrinopileatus culture medium.
Background technology
Pleurotus citrinopileatus (latin name Pleurotus citrinopileatus) also known as gold oyster mushroom, Jade Emperor mushroom, goldentop mushroom;For
Agaricales, Pleurotaceae, gold oyster mushroom category.It is wooden corruption edible mushroom.It is distributed in the ground such as Heilungkiang, Jilin, Liaoning, Hebei, Guangdong.
Wild gold oyster mushroom is more with the Northeast.
Elm mushroom nutrition is very high, containing a large amount of protein, fat, carbohydrate and mineral matter, and multivitamin and
The organic substances such as freshener sodium glutamate, the title for having " plain meat " is rare delicious food in cooking dishes, can fry, it is explosive, can
Burn, can take off, can stew, its delicious flavour, mouthfeel slides tender elm mushrooms delicious flavour, nutritious, containing protein, vitamin and
The multiple nutritional components such as mineral matter, wherein amino acid content are particularly enriched, and essential amino acids content is high.Category high nutrition, low-heat
Amount food, long-term consumption has the function of the cholesterol level that reduces blood pressure, reduces, and is the elderly's cardiovascular patient and obesity
The preferable health food of disease patient.Can be used as medicine, it is the northeast having long enjoyed a good reputation to control weakness disease elm mushrooms such as disease (flesh withers) and dysentery of withering
Local local and special products, it is beautiful in colour, fine figure, smell mellowness, and quality is tender and crisp, delicate mouthfeel, is a kind of rare edible mushroom
And medicinal fungus.Elm mushroom is the northeast ground necromancer special product having long enjoyed a good reputation, and it is beautiful in colour, and fine figure, smell mellowness, quality are crisp
Tender, delicate mouthfeel, is a kind of rare edible mushroom and medicinal fungus.Elm mushroom is rich in protein, and amino acid and vitamin etc. are various
Nutrition, containing 17 kinds of amino acid, rich in the necessary 8 kinds of amino acid of human body, especially content of glutamic acid and mushroom class content most
Straw mushroom high is identical, and containing abundant potassium, sodium, calcium, iron, zinc, and vitamin C, nicotinic acid, pantothenic acid etc. are rare treasure
Your edible mushroom.
But, Pleurotus citrinopileatus amino acid content at present on the market is not high, it is impossible to meet consumer demand.
The content of the invention
It is an object of the invention to provide a kind of preparation method of Pleurotus citrinopileatus culture medium, by the time and the bar that design fermentation
Part is controlled, and the Pleurotus citrinopileatus culture medium of preparation is improved the amino acid content and micronutrient levels of Pleurotus citrinopileatus.
A kind of preparation method of Pleurotus citrinopileatus culture medium that the present invention is provided, comprises the following steps:
1), after by 20-30 parts, the cotton stalk 30-40 parts and corncob 25-35 parts mixing of willow wood chip, 50-100 parts of water is added,
Build heap fermentation;
2) after, fermenting, rare earth element solution 0.001-0.002 parts is sprinkled, continues to ferment;
3) urea 2-3 parts, ammonium sulfate 0.5-1 parts, magnesium sulfate 0.02-0.05 parts, calcium superphosphate 0.02-0.2, are subsequently adding
Part, sodium selenite 0.02-0.1 part and water 10-30 parts, build heap ferment;
4) and then, add 0.2-3 part of lime, 0.3-0.8 parts of sugar, build heap ferment, obtain Pleurotus citrinopileatus culture medium.
Step 1) described in build heap fermentation, specially:
Stockpile into 1.0m-1.2m high, 2m-3m wide, the trapezoidal heap that length is not limited, the compost per heap are controlled in dry weight
300kg-400kg, after carrying out heap, surrounding is patted, then some vertical stomatas are inserted on heap with the wooden stick of diameter 10cm-15cm,
Straight through reactor bottom, stomatal frequency is 1/m3, straw mat is finally covered by its spontaneous fermentation;After material temperature reaches 60-65 DEG C, turn over daily
Heap once, ferments 4-5 days.
Step 1) described in willow wood chip preparation method be:By willow branch and/or trunk, its quality 0.22-0.5% is spread
Pulverized limestone, be deposited in 5-12 month in the environment of aeration-drying, then be broken into the particle of particle diameter 2mm-3mm, it is standby.
Step 1) described in after cotton stalk and corncob dry, be broken into the particle of particle diameter 2mm-3mm, it is standby.
Step 2) described in ferment, specially:
After sprinkling rare earth element solution, ferment 2-3 days;
Step 2) described in rare earth element solution concentration be 0.001-0.01mmol/L.
Step 2) described in rare earth element solution be lanthanum nitrate hexahydrate or cerous nitrate solution.
Step 3) described in build heap fermentation, specially:After building heap, air humidity 60-70% is controlled, keep ventilation, fermentation
3-4 days, daily turning was once.
Step 4) described in build heap fermentation, specially:Control air humidity 65-75%, keeps ventilation, ferments 3-4 days, often
Its turning is once.
In the present invention, cotton stalk and corncob are added in cultivation base, it contains protein, amino acid and abundant phosphorus unit
Element, for the growth of Pleurotus citrinopileatus provides abundant nitrogen source, phosphorus source and amino acid.Willow wood chip provides carbon required for Pleurotus citrinopileatus growth
Source, adds urea, ammonium sulfate for the growth of Pleurotus citrinopileatus provides nitrogen source.By cotton stalk, corncob, willow wood chip, urea and sulfuric acid
The amount ratio design of ammonium, carbon source/nitrogen source reasonable mixture ratio is conducive to improving the amino acid content of Pleurotus citrinopileatus.And, addition calcium salt,
Magnesium salts and selenium salt, improve the micro- content of Pleurotus citrinopileatus;Rare earth element is added, the growth of Pleurotus citrinopileatus can not only be promoted,
And, acted synergistically with trace element, suppress the absorption of Pleurotus citrinopileatus heavy metal element, make Pleurotus citrinopileatus more healthy.
In preparation process, by four fermentations, different raw materials are added after fermentation every time.It is firstly added cotton stalk, corn
Core, willow wood chip.Willow is added pulverized limestone other side 5-10 month after drying, and has both degraded poisonous bateriostatics, and lignin, fibre are made again
The macromolecular substances such as dimension element are decomposed into the simple material for being conducive to Pleurotus citrinopileatus to absorb, and promote the growth of Pleurotus citrinopileatus;In addition,
When 3 times and the 4th are fermented, moisturizing humidity and ventilation condition, so that the material of fermentation will not go mouldy, maintain its highest nutrient.
Compared with prior art, the cultivation base comprehensive nutrition that the present invention is provided, is conducive to the growth of Pleurotus citrinopileatus, and, energy
The amino acid content of Pleurotus citrinopileatus is enough improved, its health care and medical value is improved.
Specific embodiment
Embodiment 1
A kind of Pleurotus citrinopileatus culture medium, contains following raw material:
The Pleurotus citrinopileatus culture medium preparation method is:
1) after, mixing 31 parts of 22 parts of willow wood chip, 32 parts of cotton stalk and corncob, 80 parts of water is added, by stockpile into height
1.0m-1.2m, 2m-3m wide, the trapezoidal heap that length is not limited, the compost per heap are controlled in dry weight 300kg-400kg, carry out heap
Afterwards, surrounding is patted, then some vertical stomatas, straight through reactor bottom is inserted on heap with the wooden stick of diameter 10cm-15cm, stomatal frequency is
1/m3, straw mat is finally covered by its spontaneous fermentation;After material temperature reaches 60-65 DEG C, daily turning once, is fermented 4-5 days;
2) after, fermenting, concentration is sprinkled for 0.001 part of 0.001mmol/L lanthanum nitrate hexahydrates, continue fermentation 2-3 days;
3) 2.5 parts of urea, 0.6 part of ammonium sulfate, 0.03 part of magnesium sulfate, 0.1 part of calcium superphosphate, sodium selenite, are subsequently adding
0.08 part and 10 parts of water, after building heap, control air humidity 60-70%, keep ventilation, ferment 3-4 days, and daily turning is once;
4) and then, add 1.2 parts of lime, sugar 05 part, control air humidity 65-75%, keep ventilation, ferment 3-4 days,
Daily turning once, obtains Pleurotus citrinopileatus culture medium.
The Pleurotus citrinopileatus amino acid content of the Pleurotus citrinopileatus medium culture of preparation is higher than the Pleurotus citrinopileatus of common cultivation, especially paddy
Histidine content improves 42% than the content of common cultivation.
Embodiment 2
A kind of Pleurotus citrinopileatus culture medium, contains following raw material:
Cerous nitrate solution concentration is 0.003mmol/L.
Preparation method is with embodiment 1.
The Pleurotus citrinopileatus amino acid content of the Pleurotus citrinopileatus medium culture of preparation is higher than the Pleurotus citrinopileatus of common cultivation, especially paddy
Histidine content improves 37% than the content of common cultivation.
Claims (9)
1. a kind of preparation method of Pleurotus citrinopileatus culture medium, it is characterised in that the preparation method is comprised the following steps:
1), after by 20-30 parts, the cotton stalk 30-40 parts and corncob 25-35 parts mixing of willow wood chip, 50-100 parts of water is added, builds heap
Fermentation;
2) after, fermenting, rare earth element solution 0.001-0.002 parts is sprinkled, continues to ferment;
3), be subsequently adding urea 2-3 parts, ammonium sulfate 0.5-1 parts, magnesium sulfate 0.02-0.05 parts, calcium superphosphate 0.02-0.2 parts,
Sodium selenite 0.02-0.1 parts and water 10-30 parts, build heap fermentation;
4) and then, add 0.2-3 part of lime, 0.3-0.8 parts of sugar, build heap ferment, obtain Pleurotus citrinopileatus culture medium.
2. the preparation method of Pleurotus citrinopileatus culture medium according to claim 1, it is characterised in that step 1) described in build heap hair
Ferment, specially:
Stockpile into 1.0m-1.2m high, 2m-3m wide, the trapezoidal heap that length is not limited, the compost per heap are controlled in dry weight 300kg-
400kg, after carrying out heap, surrounding is patted, then some vertical stomatas, straight through reactor are inserted on heap with the wooden stick of diameter 10cm-15cm
Bottom, stomatal frequency is 1/m3, straw mat is finally covered by its spontaneous fermentation;After material temperature reaches 60-65 DEG C, daily turning one
It is secondary, ferment 4-5 days.
3. the preparation method of Pleurotus citrinopileatus culture medium according to claim 1 and 2, it is characterised in that step 1) described in poplar
Trees consider preparation method to be worth doing:By willow branch and/or trunk, the pulverized limestone of its quality 0.22-0.5% is spread, be deposited in ventilation dry
Individual month of 5-12 in dry environment, then the particle of particle diameter 2mm-3mm is broken into, it is standby.
4. the preparation method of the Pleurotus citrinopileatus culture medium according to claim any one of 1-3, it is characterised in that step 1) in institute
State cotton stalk and after corncob is dried, be broken into the particle of particle diameter 2mm-3mm, it is standby.
5. the preparation method of Pleurotus citrinopileatus culture medium according to claim 1, it is characterised in that step 2) described in ferment,
Specially:
After sprinkling rare earth element solution, ferment 2-3 days.
6. the preparation method of Pleurotus citrinopileatus culture medium according to claim 1, it is characterised in that step 2) described in rare earth unit
Plain solution concentration is 0.001-0.01mmol/L.
7. the preparation method of the Pleurotus citrinopileatus culture medium according to claim 1 or 6, it is characterised in that step 2) described in it is dilute
Earth elements solution is lanthanum nitrate hexahydrate or cerous nitrate solution.
8. the preparation method of Pleurotus citrinopileatus culture medium according to claim 1, it is characterised in that step 3) described in build heap hair
Ferment, specially:After building heap, air humidity 60-70% is controlled, keep ventilation, fermented 3-4 days, daily turning is once.
9. the preparation method of Pleurotus citrinopileatus culture medium according to claim 1, it is characterised in that step 4) described in build heap hair
Ferment, specially:Control air humidity 65-75%, keeps ventilation, ferments 3-4 days, and daily turning is once.
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