CN102701823A - Production method of selenium-rich high-quality honeysuckle - Google Patents
Production method of selenium-rich high-quality honeysuckle Download PDFInfo
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- CN102701823A CN102701823A CN201210187141XA CN201210187141A CN102701823A CN 102701823 A CN102701823 A CN 102701823A CN 201210187141X A CN201210187141X A CN 201210187141XA CN 201210187141 A CN201210187141 A CN 201210187141A CN 102701823 A CN102701823 A CN 102701823A
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Abstract
The invention belongs to the technical field of plant cultivation, and provides a production method of selenium-rich high-quality honeysuckle. The production method comprises the following steps of: mixing farmyard manure and sodium selenite, and applying the mixture onto a root part of the honeysuckle when in budding dates of the honeysuckle; adding rare earth micronutrients, sodium selenite, sodium hydrogen sulfite and surface active agent into water, stirring and dissolving, and spraying the dissolved mixture onto leaf surfaces when in honeysuckle flourishing period and bud stage; and airing the collected honeysuckle to obtain a honeysuckle product. Due to a rear earth-amino acid binary coordination compound, the function of a plant growth regulator is achieved; the content of heavy metal ion is reduced; the contents of effective component chlorogenic acid and galuteolin are improved; the yield and the quality of the honeysuckle are improved further; and the sodium hydrogen sulfite provides an acid environment for the honeysuckle, so that the absorption on selenium elements is promoted. The production method of the selenium-rich high-quality honeysuckle adopts a biotransformation method so as to transform inorganic selenium to organic selenium; solves the problem that the honeysuckle produced by a traditional planting method only contains basic effective components, so as not to reach the aim of supplement of selenium after being eaten; and can be used for batch production of the honeysuckle.
Description
Technical field
The invention belongs to the plant cultivation technical field, relate in particular to the working method of a kind of rich selenium high-quality Japanese Honeysuckle.
Background technology
Selenium is the essential micronutrient element of animal and human's class.The international selenium recommendation daily intaking amount 60-400 μ g of association, daily intaking amount 50-200 μ g recommends in Chinese Soclety of Nutrition.And according to China's nutrition survey, the selenium intake of being grown up every day is 26-32 μ g only.Therefore, the population of China selemium nutrition is bad to be universal phenomenon, and China lacks selenium soil and accounts for the whole nation 72%, lacks selenium population and reaches more than 100,000,000.
Science is verified now; Selenium has the effect of more than 400 kind of diseases such as anti-cancer, anticancer, control cardiovascular and cerebrovascular diseases, hepatopathy, mellitus, gynecopathy, prostate gland and cataract; Have anti-oxidant, remove that interior free yl improves immunizing power, many specific functions delay senility; Go down and aging course etc. as cardiovascular and cerebrovascular diseases, cancer, mellitus, body immunity, all relevant with scarce selenium.And under the serious situation, selenium has special physiological functions such as the heavy metal poisoning of releasing day by day in environmental pollution.Therefore, human body is mended selenium and is not only the requirement that improves immunizing power, also is the effective way that strengthens the resistibility of environmental poisonous substance; Selenium in the natural food can not satisfy the normal need of human body, and economic, healthy, safe selenium-enriched food is human effective way of mending selenium.Animal is mended selenium and adds and food with Sodium Selenite in the past, but its biological effectiveness is low, and takes in narrow range, be prone to produce excess intake and the problem of poisoning, and rich by reference selenium Lonicera flower tea can be when leisure be had tea easy to do supplement selenium element.
Research confirms that REE does not belong to the nutritive element of plant, and rationally using rare earth, to impel increasing crop yield and fine reason mainly be that rare earth belongs to the physiologically active of plant material; Have the function of regulating physiological activity in the plant materials, can promote farm crop, increase the chloroplast(id) content of plant nutrient absorbing such as nitrogen, phosphorus, potassium; Improve intensity of photosynthesis, promote enzymic activity, promote the crop floral organ to grow; Early flowering; Suitable rare earth concentration can promote seed germination to promote seed germination and root growth, reduces the crop sickness rate, the anti-adversity ability of enhancing crop.Rare earth is residual little, is a kind of important plant-growth regulator that is worthy to be popularized.Therefore, the someone thinks that rare earth application has been most important applications exploitation since rare earth is found 200 years.
In recent years, along with going deep into of rare earth application theory and practice, people begin REE is used for herbal medicine, to improving herbal medicine output and quality, improve the content of Chinese herbal medicine effective ingredients undoubtedly, finally improve curative effect of medication, have opened up new way.Research is illustrated in uses rare earth on the herbal medicine, can promote root growth, stratification, reduces disease; Improve seedling rate; And quicken seedling and grow tall and increase slightly, improve chlorophyll content, promote nitrogen, phosphorus, potassium absorbs, and improves soluble sugar content and content of amino acids; Improve quality, strengthen plant resistance.Use the trophic level that rare earth can obviously improve herbal medicine, promote growing of plant, increase dry-matter accumulation and chlorophyll content, obviously reduce fruit drop.
Summary of the invention
The invention provides the working method of a kind of rich selenium high-quality Japanese Honeysuckle; Be intended to solve in the Japanese Honeysuckle of prior art production; The content and the validity of trace element-selenium are lower, and effective component chlorogenicacid and galuteolin content are lower, the problem that security of Japanese Honeysuckle and economy are relatively poor.
The object of the present invention is to provide the working method of a kind of rich selenium high-quality Japanese Honeysuckle, this working method may further comprise the steps:
Step 1 is at the sprouting period of Japanese Honeysuckle, with imposing on the Japanese Honeysuckle root after farm manure and the Sodium Selenite mixing;
Step 2, RE microbial fertilizer, Sodium Selenite, sodium sulfite anhy 96 and the tensio-active agent stirring and dissolving that is added to the water is sprayed the dissolved mixed solution at the prosperous liquid level that carries out for a long time of Japanese Honeysuckle;
Step 3, is sprayed on the Japanese Honeysuckle with the mixed solution for preparing in the step 2 rice flower bud phase Japanese Honeysuckle;
Step 4 is dried the Japanese Honeysuckle after gathering, and obtains the Japanese Honeysuckle finished product.
Further, the weight ratio of farm manure and Sodium Selenite is farm manure: Sodium Selenite=100: 0.001-0.2; The fertilising consumption is the every pier of 2-3kg/.
Further, with RE microbial fertilizer, Sodium Selenite, sodium sulfite anhy 96 and the tensio-active agent stirring and dissolving that is added to the water, the implementation method that obtains mixed solution is:
Mix with 10 parts of weight ratio RE microbial fertilizers, Sodium Selenite 0.01-0.04 part, sodium sulfite anhy 96 0.05-2 part;
It is that 20-50 ℃, weight ratio are in 100 parts the water that fertilizer after mixing is joined temperature, and adds the tensio-active agent stirring and dissolving, obtains mixed solution.
Further, the RE microbial fertilizer staple is nitrogen, phosphorus, potassium and rare earth-amino acid binary complex, the N in the RE microbial fertilizer: P
2O
5: K
2O: the weight ratio of rare earth-amino acid binary complex is 20: 10: 10: 0.001-0.05.
Further; The general formula of the rare earth in the RE microbial fertilizer-amino acid binary complex is REx-(AA) y; X=1-2 wherein, y=1-4, the rare earth RE in the formula are any one trivalent ions among La, Ce, Pm, the Nd; (AA) in the formula is amino acid, is selected from Methionin, Threonine, glycocoll, leucine, Isoleucine, Xie Ansuan, methionine(Met), tyrosine, tryptophane, phenylalanine(Phe).
Further, at the sprouting period of Japanese Honeysuckle, with the implementation method that imposes on the Japanese Honeysuckle root after farm manure and the Sodium Selenite mixing be: the mixture of farm manure and Sodium Selenite is executed being embedded in the topsoil 20cm, the shop thin soil covers above.
Further, when spraying the mixed solution of forming by RE microbial fertilizer, Sodium Selenite, sodium sulfite anhy 96 and tensio-active agent, should be chosen in after cloudy day or fine at 4 o'clock in afternoon, spray in back 24 hours,, fill spray 1 time in case of rainy in the rice flower bud phase of Japanese Honeysuckle.
Further, the rare earth in RE microbial fertilizer-amino acid binary complex is La (Gly)
4Cl
33H
2During O, rare earth-amino acid binary complex La (Gly)
4Cl
33H
2The compound method of O is:
Take by weighing a certain amount of La
2O
3Solid is dissolved among the HCl, lets it fully react and in water-bath, slowly is evaporated to dried;
Use washing with acetone, be placed on and be dried to constant weight in the NaOH moisture eliminator and make LaCl
3Hydrate;
Accurately take by weighing 1mmoL LaCl
3Hydrate adds the 25mL absolute ethyl alcohol, stirs down, to the aqueous solution 3mL that wherein drips the 4mmoL glycocoll;
Stir suction filtration after 0.5 hour, use washing with alcohol, on silica gel, be dried to constant weight and make rare earth-amino acid binary complex La (Gly)
4Cl
33H
2O.
The working method of rich selenium high-quality Japanese Honeysuckle provided by the invention is at the sprouting period of Japanese Honeysuckle, with imposing on the Japanese Honeysuckle root after farm manure and the Sodium Selenite mixing; RE microbial fertilizer, Sodium Selenite, sodium sulfite anhy 96 and the tensio-active agent stirring and dissolving that is added to the water is carried out liquid level with the dissolved mixed solution in prosperous long-term and rice flower bud phase of Japanese Honeysuckle and is sprayed; Japanese Honeysuckle to after gathering is dried, and obtains the Japanese Honeysuckle finished product; The effect of plant-growth regulator is played in the use of rare earth-amino acid binary complex, compares with the little fertilizer of traditional rare earth, and interior suction conductivity is high; The metabolism of ability coordinate plant growth promotes the absorption of plant to organic in the environment and inorganic nutrients, increases chlorophyll content; Improve photosynthesis; Promote dry-matter transhipment, accumulation and secondary metabolism, reduce heavy metal ion content, improve yield and quality.Not reducing its high-quality effect of increasing production simultaneously, obviously reduce using dosage, improved the security of agriculture prodn thus.The use of sodium sulfite anhy 96 provides sour environment, promotes the absorption of crop to the selenium element, and can suppress photorespiration, promotes dry-matter accumulation; The working method of general rich selenium high-quality Japanese Honeysuckle is fully through the absorption in the Japanese Honeysuckle process of growth; Adopt the method for bio-transformation that inorganic selenium is converted into organoselenium; The Japanese Honeysuckle that has solved traditional cultivation method production well only contains basic effective component, does not reach the problem of mending selenium after eating, and is safe and reliable; Can carry out the production in enormous quantities of Japanese Honeysuckle, have stronger popularization and using value.
Description of drawings
Fig. 1 is the realization flow figure of the working method of the rich selenium high-quality Japanese Honeysuckle that provides of the embodiment of the invention.
Embodiment
In order to make the object of the invention, technical scheme and advantage clearer,, the present invention is further specified below in conjunction with accompanying drawing and embodiment.Should be appreciated that specific embodiment described herein only in order to explanation the present invention, and be not used in the qualification invention.
Fig. 1 shows the realization flow of the working method of the rich selenium high-quality Japanese Honeysuckle that the embodiment of the invention provides.
This working method may further comprise the steps:
In step S101, at the sprouting period of Japanese Honeysuckle, with imposing on the Japanese Honeysuckle root after farm manure and the Sodium Selenite mixing;
In step S102, RE microbial fertilizer, Sodium Selenite, sodium sulfite anhy 96 and the tensio-active agent stirring and dissolving that is added to the water is sprayed the dissolved mixed solution at the prosperous liquid level that carries out for a long time of Japanese Honeysuckle;
In step S103, the rice flower bud phase Japanese Honeysuckle, be sprayed on the Japanese Honeysuckle with the mixed solution for preparing among the step S102;
In step S104, the Japanese Honeysuckle after gathering is dried, obtain the Japanese Honeysuckle finished product.
In embodiments of the present invention, the weight ratio of farm manure and Sodium Selenite is farm manure: Sodium Selenite=100: 0.001-0.2; The fertilising consumption is the every pier of 2-3kg/.
In embodiments of the present invention, with RE microbial fertilizer, Sodium Selenite, sodium sulfite anhy 96 and the tensio-active agent stirring and dissolving that is added to the water, the implementation method that obtains mixed solution is:
Mix with 10 parts of weight ratio RE microbial fertilizers, Sodium Selenite 0.01-0.04 part, sodium sulfite anhy 96 0.05-0.2 part;
It is that 20-50 ℃, weight ratio are in 100 parts the water that fertilizer after mixing is joined temperature, and adds the tensio-active agent stirring and dissolving, obtains mixed solution.
In embodiments of the present invention, the RE microbial fertilizer staple is nitrogen, phosphorus, potassium and rare earth-amino acid binary complex, the N in the RE microbial fertilizer: P
2O
5: K
2O: the weight ratio of rare earth-amino acid binary complex is 20: 10: 10: 0.001-0.05.
In embodiments of the present invention; The general formula of the rare earth in the RE microbial fertilizer-amino acid binary complex is REx (AA) y; X=1-2 wherein, y=1-4, the rare earth RE in the formula are any one trivalent ions among La, Ce, Pm, the Nd; (AA) in the formula is amino acid, is selected from Methionin, Threonine, glycocoll, leucine, Isoleucine, Xie Ansuan, methionine(Met), tyrosine, tryptophane, phenylalanine(Phe).
In embodiments of the present invention, at the sprouting period of Japanese Honeysuckle, with the implementation method that imposes on the Japanese Honeysuckle root after farm manure and the Sodium Selenite mixing be: the mixture of farm manure and Sodium Selenite is executed being embedded in the topsoil 20cm, the shop thin soil covers above.
In embodiments of the present invention; When spraying the mixed solution of forming by RE microbial fertilizer, Sodium Selenite, sodium sulfite anhy 96 and tensio-active agent, should be chosen in after cloudy day or fine at 4 o'clock in afternoon, spray in back 24 hours in the prosperous long-term and rice flower bud phase of Japanese Honeysuckle; In case of rainy, fill spray 1 time.
In embodiments of the present invention, the rare earth in RE microbial fertilizer-amino acid binary complex is La (Gly)
4Cl
33H
2During O, rare earth-amino acid binary complex La (Gly)
4Cl
33H
2The compound method of O is:
Take by weighing a certain amount of La
2O
3Solid is dissolved among the HCl, lets it fully react and in water-bath, slowly is evaporated to dried;
Use washing with acetone, be placed on and be dried to constant weight in the NaOH moisture eliminator and make LaCl
3Hydrate;
Accurately take by weighing 1mmoL LaCl
3Hydrate adds the 25mL absolute ethyl alcohol, stirs down, to the aqueous solution 3mL that wherein drips the 4mmoL glycocoll;
Stir suction filtration after 0.5 hour, use washing with alcohol, on silica gel, be dried to constant weight and make rare earth-amino acid binary complex La (Gly)
4Cl
33H
2O.
Below in conjunction with accompanying drawing and specific embodiment application principle of the present invention is further described.
As shown in Figure 1, this production is biological selenium-enriched, and good quality and high output Japanese Honeysuckle method is undertaken by following step:
A, impose on root after when the fertilising of the sprouting period of Japanese Honeysuckle, adopting farm manure and Sodium Selenite to mix, the weight ratio of Sodium Selenite and farm manure is farm manure: Sodium Selenite=100: 0.001-0.2; The fertilising consumption is the every pier of 2-3kg/;
B, leaf stretching period spray: leaf of Flos Lonicerae is grown prosperous when long, is carrier with the RE microbial fertilizer, adds Sodium Selenite, sodium sulfite anhy 96; With 10 parts of weight ratio RE microbial fertilizers, Sodium Selenite 0.01-0.04 part, sodium sulfite anhy 96 0.05-0.2 part is mixed; Mixed fertilizer joins temperature 20-50 ℃; Weight ratio is in 100 parts the water, and adds the tensio-active agent stirring and dissolving, and the dissolved mixed solution carries out liquid level and sprays;
C, rice flower bud phase spray: the rice flower bud phase Japanese Honeysuckle, with the mixed solution for preparing among the step b, be sprayed on the Japanese Honeysuckle;
After handling through above-mentioned steps, dry after Japanese Honeysuckle is gathered and get product.
The mixture of farm manure and Sodium Selenite is executed in topsoil 20cm among the above-mentioned steps a, and the shop thin soil covers above;
The little fertilizer of above-mentioned steps b middle-weight rare earths is mainly the mixture of nitrogen, phosphorus, potassium and rare earth-amino acid binary complex, the N in this RE microbial fertilizer: P
2O
5: K
2O: the weight ratio of rare earth-amino acid binary complex is 20: 10: 10: 0.01-0.5;
Among the above-mentioned steps b, the general formula of the rare earth in the RE microbial fertilizer-amino acid binary complex is REx (AA) y, wherein x=1-2; Y=1-4, the rare earth RE in the formula is La, Ce; Pm; Any one trivalent ion among the Nd, (AA) in the formula is amino acid, is selected from Methionin, Threonine, glycocoll, leucine, Isoleucine, Xie Ansuan, methionine(Met), tyrosine, tryptophane, phenylalanine(Phe).
Embodiment 1:
The present invention is a kind of method of producing rich selenium, good quality and high output Japanese Honeysuckle; The first step: at first when the sprouting period fertilising of Japanese Honeysuckle; Execute at the Japanese Honeysuckle root after adopting farm manure and Sodium Selenite to mix; Farm manure mixes with Sodium Selenite through dry with poultry, livestock or people's ight soil, and the weight ratio of farm manure and Sodium Selenite is: farm manure: Sodium Selenite=100: 0.005; The fertilising consumption is the every pier of 3kg/; Farm manure is a natural materials, can required nutritive substance be provided for the growth of gold and silver flower seedling, but must mix with Sodium Selenite dry back, otherwise Sodium Selenite is prone to solution loss;
The mixture of farm manure and Sodium Selenite is executed and is embedded in the topsoil 20cm, and the shop thin soil covers above.Sodium Selenite is prone to be dissolved in the moisture in the soil, and is easy to flow away downwards, crops seedling stage, and thin soil covering in shop is the effect of playing secluding air above, avoids oxidized.
Second step, prosperous long-term Japanese Honeysuckle, should strengthen benefit selenium, and regulate with rare earth-amino acid plant-growth regulator.With the RE microbial fertilizer is carrier, adds Sodium Selenite, sodium sulfite anhy 96, with 10 parts of weight ratio RE microbial fertilizers; Sodium Selenite 0.001-0.04 part; Sodium sulfite anhy 96 0.05-0.2 part is mixed, and mixed fertilizer joins temperature 20-50 ℃, and weight ratio is in 100 parts the water; And adding the organic surface active agent stirring and dissolving, the dissolved mixed solution carries out liquid level and sprays.RE microbial fertilizer is the laboratory preparation, wherein, and N: P
2O
5: K
2The weight ratio of O is 20: 10: 10, adds rare earth-amino acid binary complex La (Gly) again
4Cl
33H
2O;
The selenium element need repeatedly spray, but the selenium element is easier to by plant absorbing under sour environment through liquid level specific absorption difficulty, and sodium sulfite anhy 96 can provide more weak sour environment, can promote the absorption of crop to selenium; Rare earth-amino acid binary complex La (Gly)
4Cl
33H
2The compound method of O: take by weighing a certain amount of La
2O
3Solid is dissolved among the HCl, lets it fully react and in water-bath, slowly is evaporated to driedly, uses washing with acetone then, is placed on to be dried to constant weight in the NaOH moisture eliminator and to make LaCl
3Hydrate.Accurately take by weighing 1mmoL LaCl
3Hydrate adds the 25mL absolute ethyl alcohol, stirs down, to the aqueous solution 3mL that wherein drips the 4mmoL glycocoll, stirs suction filtration after 0.5 hour, uses washing with alcohol, on silica gel, is dried to constant weight and makes title complex.Through analyze this binary complex consist of La (Gly)
4Cl
33H
2O.
With Sodium Selenite, rare earth-amino acid binary complex fertilizer, sodium sulfite anhy 96 proportional mixing, be dissolved in 20-50 ℃ the water, and add a certain amount of organic surface active agent, Japanese Honeysuckle is implemented liquid level spray.
In the 3rd step, the rice flower bud phase sprays: in the rice flower bud phase of Japanese Honeysuckle, the mixed solution for preparing among the b set by step is sprayed on the Japanese Honeysuckle.
In second step and the operation of the 3rd step, during sprinkling, the choosing cloudy day, or after fine at 4 o'clock in afternoon, spray in back 24 hours, in case of rainy, fill spray 1 time.
After handling through above-mentioned steps, dry after Japanese Honeysuckle is gathered and get product.
Through the Japanese Honeysuckle product after the above-mentioned steps processing, selenium content is 786 μ g/kg through measuring wherein, and chlorogenicacid and galuteolin content increase 5-15% respectively than untreated sample, and output increases 5-22%.
Embodiment 2:
The production technology of a kind of biological selenium-enriched, good quality and high output Japanese Honeysuckle of the present invention.
At first when the sprouting period fertilising of Japanese Honeysuckle, adopt farm manure and Sodium Selenite to mix the back root and execute at the Japanese Honeysuckle root farm manure: Sodium Selenite=100: 0.01; The fertilising consumption is the every pier of 2kg/; The mixture of farm manure and Sodium Selenite is executed and is embedded in the topsoil 20cm, and the shop thin soil covers above.
Secondly, gold and silver flower seedling prosperous long-term (i.e. about 30 days before rice flower bud phase), strengthen and mend selenium, and regulate with rare earth-amino acid plant-growth regulator.With the weight ratio is RE microbial fertilizer 20kg, wherein includes La (Gly)
4Cl
33H
2O 0.005kg adds Sodium Selenite 0.001-0.006kg, and bisulfite 0.005-0.025kg mixes; Get mixed fertilizer 0.05kg fertilizer and join temperature 20-50 ℃; Weight ratio is in the water of 100kg, and adds the organic surface active agent stirring and dissolving, and the dissolved mixed solution carries out liquid level and sprays.RE microbial fertilizer is the laboratory preparation, wherein, and N: P
2O
5: K
2The weight ratio of O is 20: 10: 10, adds rare earth-amino acid binary complex La (Gly) again
4Cl
33H
2O.
At last, the rice flower bud phase sprays: in the rice flower bud phase of Japanese Honeysuckle, the mixed solution for preparing among the b set by step is sprayed on the Japanese Honeysuckle.
In the two steps operation of back, during sprinkling, the choosing cloudy day, or after fine at 4 o'clock in afternoon, spray in back 24 hours, in case of rainy, fill spray 1 time.
After handling through above-mentioned steps, dry after Japanese Honeysuckle is gathered and get product.
Through the Japanese Honeysuckle product after the above-mentioned steps processing, selenium content is 762 μ g/kg through measuring wherein, and chlorogenicacid and galuteolin content increase 9-19% respectively than untreated sample, and output increases 8-21%.
By above method, with containing 30mgL
-1The glycocoll lanthanum handle Pingyi pan honeysuckle flower (Lonicera confusa DC.), can adopt following method to carry out chlorogenicacid and galuteolin Determination on content.
The mensuration of chlorogenicacid:
Chromatographic condition: with the octadecylsilane chemically bonded silica is weighting agent, is moving phase with acetonitrile-0.4% phosphoric acid solution (13: 87), detects 327nm, is the characteristic peak of 17.9min place for chlorogenicacid in RT.
The preparation of need testing solution: the about 0.5g of extracting honeysuckle powder (crossing sieve No. four), the accurate title, decide, and puts in the tool plug Erlenmeyer flask, and the accurate 50% methyl alcohol 50mL that adds claims to decide weight; Supersound process (power 250w, frequency 35Hz) 30min is put coldly, claims to decide weight again; Supply the weight that subtracts mistake with 50% methyl alcohol, shake up, filter, precision is measured subsequent filtrate 5mL; Put in the 25mL brown bottle, add 50% methyl alcohol, shake up, promptly get to scale.The accurate respectively reference substance 10 μ L that draw inject liquid chromatograph, measure, and promptly get.Recording without chlorogenic acid content in the Japanese Honeysuckle of glycocoll lanthanum processing is 2.12%, through containing 30mgL
-1The glycocoll lanthanum Japanese Honeysuckle of handling in chlorogenic acid content be 2.34%, comparison is according to having increased by 10.38%.
The mensuration of galuteolin:
Chromatographic condition: with the octadecylsilane chemically bonded silica is weighting agent, is mobile phase A with the acetonitrile, is Mobile phase B with 0.5% glacial acetic acid solution; Press 0-30min; Mobile phase A: 10%-30%, Mobile phase B: 90%-70% carries out gradient elution, and the detection wavelength is 350nm.In RT is the characteristic peak of 48.1min place for galuteolin.
The preparation of need testing solution: the about 3g of extracting honeysuckle fine powder (crossing sieve No. four), the accurate title, decide, and puts in the tool plug Erlenmeyer flask, the accurate 70% ethanol 50mL that adds; Claim to decide weight, supersound process (power 250w, frequency 35kHz) 1 hour is put cold; Claim again to decide weight, supply the weight that subtracts mistake, shake up with 70% ethanol; Filter, get subsequent filtrate, promptly get.The accurate need testing solution 10 μ L that draw inject liquid chromatograph, measure, and promptly get.Recording without galuteolin content in the Japanese Honeysuckle of glycocoll lanthanum processing is 0.14%, through containing 30mgL
-1The glycocoll lanthanum Japanese Honeysuckle of handling in galuteolin content be 0.16%, comparison is according to having increased by 14.28%.
The working method of the rich selenium high-quality Japanese Honeysuckle that the embodiment of the invention provides is at the sprouting period of Japanese Honeysuckle, with imposing on the Japanese Honeysuckle root after farm manure and the Sodium Selenite mixing; RE microbial fertilizer, Sodium Selenite, sodium sulfite anhy 96 and the tensio-active agent stirring and dissolving that is added to the water is carried out liquid level with the dissolved mixed solution in prosperous long-term and rice flower bud phase of Japanese Honeysuckle and is sprayed; Japanese Honeysuckle to after gathering is dried, and obtains the Japanese Honeysuckle finished product; The effect of plant-growth regulator is played in the use of rare earth-amino acid binary complex, compares with the little fertilizer of traditional rare earth, and interior suction conductivity is high; The metabolism of ability coordinate plant growth promotes the absorption of plant to organic in the environment and inorganic nutrients, increases chlorophyll content; Improve photosynthesis; Promote dry-matter transhipment, accumulation and secondary metabolism, reduce heavy metal ion content, improve yield and quality.Not reducing its high-quality effect of increasing production simultaneously, obviously reduce using dosage, improved the security of agriculture prodn thus.The use of sodium sulfite anhy 96 provides sour environment, promotes the absorption of crop to the selenium element, and can suppress photorespiration, promotes dry-matter accumulation; The working method of general rich selenium high-quality Japanese Honeysuckle is fully through the absorption in the Japanese Honeysuckle process of growth; Adopt the method for bio-transformation that inorganic selenium is converted into organoselenium; The Japanese Honeysuckle that has solved traditional cultivation method production well only contains basic effective component, does not reach the problem of mending selenium after eating, and is safe and reliable; Can carry out the production in enormous quantities of Japanese Honeysuckle, have stronger popularization and using value.
The above is merely preferred embodiment of the present invention, not in order to restriction the present invention, all any modifications of within spirit of the present invention and principle, being done, is equal to and replaces and improvement etc., all should be included within protection scope of the present invention.
Claims (8)
1. the working method of a rich selenium high-quality Japanese Honeysuckle is characterized in that, this working method may further comprise the steps:
Step 1 is at the sprouting period of Japanese Honeysuckle, with imposing on the Japanese Honeysuckle root after farm manure and the Sodium Selenite mixing;
Step 2, RE microbial fertilizer, Sodium Selenite, sodium sulfite anhy 96 and the tensio-active agent stirring and dissolving that is added to the water is sprayed the dissolved mixed solution at the prosperous liquid level that carries out for a long time of Japanese Honeysuckle;
Step 3, is sprayed on the Japanese Honeysuckle with the mixed solution for preparing in the step 2 rice flower bud phase Japanese Honeysuckle;
Step 4 is dried the Japanese Honeysuckle after gathering, and obtains the Japanese Honeysuckle finished product.
2. working method as claimed in claim 1 is characterized in that, the weight ratio of farm manure and Sodium Selenite is farm manure: Sodium Selenite=100: 0.001-0.2; The fertilising consumption is the every pier of 2-3kg/.
3. working method as claimed in claim 1 is characterized in that, with RE microbial fertilizer, Sodium Selenite, sodium sulfite anhy 96 and the tensio-active agent stirring and dissolving that is added to the water, the implementation method that obtains mixed solution is:
Mix with 10 parts of weight ratio RE microbial fertilizers, Sodium Selenite 0.01-0.04 part, sodium sulfite anhy 96 0.05-2 part;
It is that 20-50 ℃, weight ratio are in 100 parts the water that fertilizer after mixing is joined temperature, and adds the tensio-active agent stirring and dissolving, obtains mixed solution.
4. working method as claimed in claim 3 is characterized in that, the RE microbial fertilizer staple is nitrogen, phosphorus, potassium and rare earth-amino acid binary complex, the N in the RE microbial fertilizer: P
2O
5: K
2O: the weight ratio of rare earth-amino acid binary complex is 20: 10: 10: 0.001-0.05.
5. working method as claimed in claim 4; It is characterized in that the general formula of the rare earth in the RE microbial fertilizer-amino acid binary complex is REx-(AA) y, wherein x=1-2; Y=1-4; Rare earth RE in the formula is any one trivalent ion among La, Ce, Pm, the Nd, and (AA) in the formula is amino acid, is selected from Methionin, Threonine, glycocoll, leucine, Isoleucine, Xie Ansuan, methionine(Met), tyrosine, tryptophane, phenylalanine(Phe).
6. working method as claimed in claim 1; It is characterized in that; At the sprouting period of Japanese Honeysuckle, with the implementation method that imposes on the Japanese Honeysuckle root after farm manure and the Sodium Selenite mixing be: the mixture of farm manure and Sodium Selenite is executed being embedded in the topsoil 20cm, the shop thin soil covers above.
7. working method as claimed in claim 1; It is characterized in that; When spraying the mixed solution of forming by RE microbial fertilizer, Sodium Selenite, sodium sulfite anhy 96 and tensio-active agent, should be chosen in after cloudy day or fine at 4 o'clock in afternoon, spray in back 24 hours in the rice flower bud phase of Japanese Honeysuckle; In case of rainy, fill spray 1 time.
8. working method as claimed in claim 5 is characterized in that, the rare earth in RE microbial fertilizer-amino acid binary complex is La (Gly)
4Cl
33H
2During O, rare earth-amino acid binary cooperates La (Gly)
4Cl
33H
2The compound method of O is:
Take by weighing a certain amount of La
2O
3Solid is dissolved among the HCl, lets it fully react and in water-bath, slowly is evaporated to dried;
Use washing with acetone, be placed on and be dried to constant weight in the NaOH moisture eliminator and make LaCl
3Hydrate;
Accurately take by weighing 1mmoL LaCl
3Hydrate adds the 25mL absolute ethyl alcohol, stirs down, to the aqueous solution 3mL that wherein drips the 4mmoL glycocoll;
Stir suction filtration after 0.5 hour, use washing with alcohol, on silica gel, be dried to constant weight and make rare earth-amino acid binary complex La (Gly)
4Cl
33H
2O.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104016752A (en) * | 2014-06-10 | 2014-09-03 | 杭州九仙生物科技有限公司 | Nutrient solution for increasing yield of pfaffia paniculata roots and application thereof |
| CN104860764A (en) * | 2015-06-02 | 2015-08-26 | 云南益硒贡宝生物工程技术有限公司 | Selenium-rich multi-element biological nutrient solution and preparation method |
| CN106386059A (en) * | 2016-08-30 | 2017-02-15 | 马健 | Planting method for honeysuckle |
| CN106748201A (en) * | 2016-12-29 | 2017-05-31 | 芜湖野树林生物科技有限公司 | A kind of preparation method of Pleurotus citrinopileatus culture medium |
| CN107244965A (en) * | 2017-06-06 | 2017-10-13 | 临沂大学 | A kind of Radix Salviae Miltiorrhizae rich in selenium composite fertilizer and the cultural method of Radix Salviae Miltiorrhizae rich in selenium |
| CN107278602A (en) * | 2017-08-07 | 2017-10-24 | 韦孺聪 | A kind of implantation methods of honeysuckle |
| US10664013B2 (en) | 2017-02-06 | 2020-05-26 | Wuhan China Star Optoelectronics Technology Co., Ltd. | Flexible display module and electronic apparatus comprising the flexible display module |
| CN112841358A (en) * | 2019-11-26 | 2021-05-28 | 衡阳仕杰农业发展有限公司 | Preparation method of selenium-added honeysuckle tea |
| CN114830977A (en) * | 2022-04-24 | 2022-08-02 | 麻城市德应米业股份有限公司 | Production method of green selenium-rich nutritional rice |
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| CN102391050A (en) * | 2011-09-18 | 2012-03-28 | 山东鲁峰金银花有限公司 | Selenium-enriched foliar fertilizer special for honeysuckle flower and preparation method thereof |
| CN102424634A (en) * | 2011-09-18 | 2012-04-25 | 山东鲁峰金银花有限公司 | Selenium-enriched special fertilizer for honeysuckle flower and production method thereof |
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| CN102424634A (en) * | 2011-09-18 | 2012-04-25 | 山东鲁峰金银花有限公司 | Selenium-enriched special fertilizer for honeysuckle flower and production method thereof |
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| CN104016752A (en) * | 2014-06-10 | 2014-09-03 | 杭州九仙生物科技有限公司 | Nutrient solution for increasing yield of pfaffia paniculata roots and application thereof |
| CN104016752B (en) * | 2014-06-10 | 2016-06-15 | 杭州九仙生物科技有限公司 | A kind of nutritional solution for improving pfaffia Root yield and application thereof |
| CN104860764A (en) * | 2015-06-02 | 2015-08-26 | 云南益硒贡宝生物工程技术有限公司 | Selenium-rich multi-element biological nutrient solution and preparation method |
| CN106386059A (en) * | 2016-08-30 | 2017-02-15 | 马健 | Planting method for honeysuckle |
| CN106748201A (en) * | 2016-12-29 | 2017-05-31 | 芜湖野树林生物科技有限公司 | A kind of preparation method of Pleurotus citrinopileatus culture medium |
| US10664013B2 (en) | 2017-02-06 | 2020-05-26 | Wuhan China Star Optoelectronics Technology Co., Ltd. | Flexible display module and electronic apparatus comprising the flexible display module |
| CN107244965A (en) * | 2017-06-06 | 2017-10-13 | 临沂大学 | A kind of Radix Salviae Miltiorrhizae rich in selenium composite fertilizer and the cultural method of Radix Salviae Miltiorrhizae rich in selenium |
| CN107278602A (en) * | 2017-08-07 | 2017-10-24 | 韦孺聪 | A kind of implantation methods of honeysuckle |
| CN112841358A (en) * | 2019-11-26 | 2021-05-28 | 衡阳仕杰农业发展有限公司 | Preparation method of selenium-added honeysuckle tea |
| CN114830977A (en) * | 2022-04-24 | 2022-08-02 | 麻城市德应米业股份有限公司 | Production method of green selenium-rich nutritional rice |
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