CN106668241A

CN106668241A - External preparation of paederia scandens extract for treating haemorrhoids

Info

Publication number: CN106668241A
Application number: CN201710173482.4A
Authority: CN
Inventors: 李仲昆
Original assignee: Individual
Current assignee: Individual
Priority date: 2017-03-22
Filing date: 2017-03-22
Publication date: 2017-05-17

Abstract

The invention provides an external preparation of a paederia scandens extract for treating haemorrhoids. When the weight ratio of the paederia scandens extract in the external preparation is 10-90%, the external preparation has an obvious therapeutical effect for haemorrhoids; when the weight ratio of the paederia scandens extract in the external preparation is 20-70%, the therapeutical effect for haemorrhoids is stronger. Compared with the prior art, the external preparation of the paederia scandens extract for treating haemorrhoids is simple in preparation, reliable in curative effect and low in cost, and does not have toxicity or side effects, therefore, the external preparation has a very high application value and is suitable for popularization and application.

Description

A kind of fevervine extract external preparation for treating hemorrhoid

Technical field

The present invention relates to a kind of fevervine extract external preparation for treating hemorrhoid, for treating hemorrhoid.

Background technology

Hemorrhoid are a kind of commonly encountered diseases, frequently-occurring disease, and main clinical manifestation is to have blood in stool, pain, edema, falling inflation, prurituss, secretions The symptom such as increase and deviate from, had a strong impact on life, working and learning, so exploitation has the hemorrhoid treating medicine of good result Thing is significant.

At present the method for the treatment of hemorrhoid mainly has Drug therapy, surgical ligation and injection treatment.Anorectal educational circles of China is clear and definite Point out that the purpose treated is not to eliminate hemorrhoid body, but eliminate symptom.These treatment concepts make non-operative treatment especially basic medicine The importance of thing treatment is burst.It is to eliminate symptom, asymptomatic hemorrhoid that the suggestion of present scholar mostly is the therapeutic purposes of hemorrhoid Without the need for treatment, Symptomatic hemorrhoid more than 90% can Jing non-operative treatment eliminate symptom, only less than 10% hemorrhoid patients need Surgical intervention.Drug therapy energy reducing swelling and alleviating pain, relief of symptoms, turn avoid the pain of operation and the complication of injection treatment, So vast majority of people is adapted to adopt Drug therapy.Drug therapy occupies critical role in the treatment of hemorrhoid, Chinese medicine preparation by In evident in efficacy and low toxicity side effect, extensively by expert and the favorable comment of patient.The Chinese medicine preparation of hemorrhoid is treated on market ointment, bolt Agent, patch, ointment, lotion, oral formulations etc., manufacturer is also numerous.That what is sold on domestic market mainly has Ma Yinglong Moschus ZHICHUAN SHUAN, cream, Gangtai Ointment, hemorrhoid-eliminating paste, jiuhua plaster, bear's gall ointment etc..There is heat-clearing and toxic substances removing, detumescence these medicines more The effects such as pain relieving, removing the necrotic tissue and promoting granulation, but still can improve in anti-inflammatory analgesic effect.

The content of the invention

It is an object of the invention to provide a kind of fevervine extract external preparation of energy effectively treatment hemorrhoid.

A kind of preparation method of the fevervine extract external preparation for treating hemorrhoid, comprises the following steps：

（1）Prepare wash clean and dried fevervine powder 1000g, in being put into decoction kettle, add deionized water, big fire to decoct 30min, then 2h is cooked by slow fire, decocting liquid is filtered, filtrate is collected, dense fluids is concentrated into, add ethanol to reach concentration of alcohol 80%, stand overnight, drying precipitate is collected, obtain 100g extracts.Except containing paederoside, Paederosidie Acid first in extract Outside fat, also containing other effective ingredient, including flavonoid, iridoidses, sterols, triterpeness etc., such as kaempferol and Cortex querci dentatae Element and its glycoside, asperuloside etc., work, therefore curative effect is good compared with single component.

（2）Extract is prepared into into suppository or ointment, wherein when the ratio of the weight containing extract is 10-90%, containing fevervine Glycosides is 0.78-7.02%, and Paederosidie Acid formicester is 0.77-6.93%；With containing extract weight ratio as 20-70% preferably.

The present invention provides the fevervine extract external preparation for the treatment of hemorrhoid, has the effect that compared with prior art： The present invention is prepared simply, and curative effect reliability has no toxic side effect, and with low cost, therefore, the present invention has very high using value, It is suitable to popularization and application.

Specific embodiment

Embodiment one

Fat-soluble suppository substrate, it is 10-90% to add fevervine extract to make weight ratio, is poured into after heating and melting in mould, is cooled down Take out.It is 0.78-7.02% to contain paederoside in through determining suppository, and Paederosidie Acid formicester is 0.77-6.93%.

Embodiment two

Fat-soluble suppository substrate, it is 20-70% to add fevervine extract to make weight ratio, is poured into after heating and melting in mould, is cooled down Take out.It is 1.56-5.46% to contain paederoside in through determining suppository, and Paederosidie Acid formicester is 1.54-5.39%.

Embodiment three

Emulsifiable paste matrix, it is 10-90% to add fevervine extract to make weight ratio, after mix homogeneously.Contain in through determining emulsifiable paste Paederoside is 0.78-7.02%, and Paederosidie Acid formicester is 0.77-6.93%.

Example IV

Emulsifiable paste matrix, it is 20-70% to add fevervine extract to make weight ratio, after mix homogeneously.Contain in through determining emulsifiable paste Paederoside is 1.56-5.46%, and Paederosidie Acid formicester is 1.54-5.39%.

The mice dimethylbenzene antiinflammatory of embodiment five is tested

48 KM mices, male and female half and half, 20 ± 2g of body weight is randomly divided into 4 groups, i.e. model control group, fevervine and extracts by body weight Thing group (concentration is 20% emulsifiable paste, and dosage is 100mg/), MAYINGLONGZHICHUANGGAO group (dosage is 100mg/), fevervine Glycosides group（Emulsifiable paste containing paederoside 20%, dosage is 100mg/）12 per group.

Experimental technique：The μ l of dimethylbenzene 20 are taken, each mouse right ear is applied to.Take after 30min each by reagent be uniformly applied to respectively it is little Mus auris dextra, auris dextra is put to death to after dimethylbenzene 3.5h, and determines the ear method of double differences, and assay method is：Two ears are cut at auricle baseline, is used 8mm card punch lays auricle at the same position of left and right ear, weighs, the ear method of double differences=auris dextra piece weight-left auricle weight.

As a result fevervine extract has obvious antiinflammatory action, and antiphlogistic effects are better than MAYINGLONGZHICHUANGGAO group, paederoside group. The ear method of double differences of model group is 6.92 ± 1.26, and the ear method of double differences of fevervine extract group is 3.89 ± 1.56, MAYINGLONGZHICHUANGGAO group The ear method of double differences is 4.77 ± 1.18, and the paederoside group ear method of double differences is 5.97 ± 1.44.

The mice analgesic activity of embodiment six is tested

KM mices 48, male and female half and half, 20 ± 2g of body weight is randomly divided into 4 groups, i.e. model control group, fevervine and extracts by body weight Thing group (concentration is 20% emulsifiable paste, and dosage is 100mg/), MAYINGLONGZHICHUANGGAO group (dosage is 100mg/), fevervine Glycosides group（Emulsifiable paste containing paederoside 20%, dosage is 100mg/）12 per group.

Experimental technique：Mice anum administration, with 10mg cotton balls 100mg medicines are stained with, and cotton balls filled in into internal rectum, and daily one Secondary, continuous three days, 30 minutes after last time administration is finished, rectal wall injected 5% formalin at anus 3.5cm 0.2ml.Writhing number of times in record mice 20 minutes.As a result fevervine extract has obvious analgesic effect, and analgesic effect is better than horse Should imperial ZHICHUANG GAO group, paederoside group.The writhing number of times of model group is 46.8 ± 11.3, the writhing number of times of fevervine extract group For 28.9 ± 16.5, MAYINGLONGZHICHUANGGAO group writhing number of times is 35.7 ± 17.4, paederoside group writhing number of times is 41.8 ± 16.3。

The experiment of the rat Oleum Tiglii hemorrhoid model of embodiment seven

48 SD rats (200 ± 20g) are randomly divided into 4 groups, i.e. model control group, fevervine extract group by body weight, and (concentration is 20% emulsifiable paste, dosage is 200mg/), MAYINGLONGZHICHUANGGAO group (dosage is 200mg/), paederoside group（Containing chicken arrow The emulsifiable paste of rattan glycosides 20%, dosage is 200mg/）12 per group.Administration：Rat carries a few days ago drop rectum with drug, secondary daily, 200mg medicines are stained with cotton balls, cotton balls is filled in into internal rectum, during administration next time, should first check whether internal rectum has cotton balls (if any cotton Ball should take out).Last time administration should be 2 hours after modeling.

Modeling：Oleum Tiglii is a kind of conventional proinflammatory agent, conventional Oleum Tiglii mixed liquor with 1 part of distilled water, 4 parts of pyridines, 5 Part ether and 10 part of 15% Oleum Tiglii diethyl ether solution are prepared.Oleum Tiglii mixed liquor is stained with 10mg cotton balls, by the cotton balls after immersion Rat anal is put into, is taken out after stopping 15 seconds.

Index determining：After modeling 7 hours, every rat intravenous injection 1% her Wen orchid normal saline solution (2ml/kg), Sacrificed by exsanguination animal after 40 minutes, cut-off intestinal and perianal tissue, use acetone normal saline 7 after weighing：3 solution soaking 48 are little When, 3000rpm is centrifuged 10 minutes, takes supernatant and surveys absorbance in 590nm, as evaluation vascular permeability index,

As a result show, fevervine extract can well reduce the rectum vein permeability of Oleum Tiglii hemorrhoid model mice, curative effect Better than MAYINGLONGZHICHUANGGAO group, paederoside group.The light absorption value of model group is 0.161 ± 0.026, the suction of fevervine extract group Light value is 0.111 ± 0.036, and the light absorption value of MAYINGLONGZHICHUANGGAO group is 0.127 ± 0.041, and paederoside group light absorption value is 0.141±0.031。

Claims

1. a kind of fevervine extract external preparation for treating hemorrhoid, it is characterised in that the weight of fevervine extract in preparation Containing paederoside it is 0.78-7.02% in preparation than for 10-90%, Paederosidie Acid formicester is 0.77-6.93%, is contained in extract There are flavonoid, iridoidses, sterols, triterpeness etc., such as kaempferol, Quercetin and its glycoside, asperuloside, fevervine Glycosides, Paederosidie Acid formicester etc., work, therefore curative effect is good compared with single component.

2. fevervine extract external preparation as claimed in claim 1, it is characterised in that the weight of fevervine extract in preparation It is 1.56-5.46% that amount contains paederoside than being 20-70%, in preparation, and Paederosidie Acid formicester is 1.54-5.39%.

3. the extract external preparation of the fevervine according to described in any of the above-described claim prepares the medicine for the treatment of hemorrhoid.