CN106619739A - Artificial collection method of body surface mucus of giant salamander - Google Patents
Artificial collection method of body surface mucus of giant salamander Download PDFInfo
- Publication number
- CN106619739A CN106619739A CN201611231165.5A CN201611231165A CN106619739A CN 106619739 A CN106619739 A CN 106619739A CN 201611231165 A CN201611231165 A CN 201611231165A CN 106619739 A CN106619739 A CN 106619739A
- Authority
- CN
- China
- Prior art keywords
- giant salamander
- mucus
- body surface
- giant
- skin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/65—Amphibians, e.g. toads, frogs, salamanders or newts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/0005—Ingredients of undetermined constitution or reaction products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/001—Use of materials characterised by their function or physical properties
- A61L24/0015—Medicaments; Biocides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/30—Compounds of undetermined constitution extracted from natural sources, e.g. Aloe Vera
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/418—Agents promoting blood coagulation, blood-clotting agents, embolising agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/04—Materials for stopping bleeding
Abstract
The invention discloses an artificial collection method of body surface mucus of a giant salamander, relating to the field of cultivation. The method comprises the steps of (a) selection of the giant salamander; (b) body surface sterilization; (c) artificial energization; (d) waiting; (e) collection of the mucus; and (f) sterilization. The body surface mucus of the giant salamander is artificially collected, the second hemostasis bonding function of the mucus of the giant salamander is utilized, and the body surface mucus is a purely natural biomaterial and is free of a toxic or side effect, so that the mucus of the giant salamander has extremely wide prospects in the field of medical operation and the like. Through collection and utilization of the mucus, the body surface mucus can be used as an excellent raw material of a medical adhesive, and can be used for other medical purposes. According to the artificial collection method of the body surface mucus of the giant salamander disclosed by the invention, by adopting artificial stimulation, the growth of the giant salamander is not affected while the mucus is collected and the additional value of giant salamander farming can be greatly improved.
Description
Technical field
The present invention relates to cultivate field, and in particular to a kind of giant salamander skin mucus artificially collect method.
Background technology
The giant salamander, scientific name giant salamander (Classification system:Andrias davidianus), it is existing in the world maximum to be also
Most precious amphibian.Like the sob of baby, therefore people are called its " giant salamander " its cry.The giant salamander is a kind of food
It is also a kind of traditional famous and precious medicinal animal with high animal is worth, its muscle, internal organ, bone, epidermis and its secretion
It is used as medicine ".
It is traditional proved recipe that giant salamander skin powder mixes tung oil and can treat burn, scald, especially to the burn and scald of face
Treatment can be with preventing from scar.Discharge after glutinous glycoprotein in giant salamander skin mucus cell, combined with water mucus, be covered in
Skin free surface, defines one natural protective barrier.Mucus main component is glutinous GL-PP and fibrous matter, also various
Hormone, and " Magainin ", with stronger antibacterial action, can prevent leprosy, senile dementia and anti-curing cancers.
Modern medical service field, many illnesss need surgery excision pathology, and in addition orthopedic damage also can be formed in human body surface
Wound, and the quick healing of wound can reduce the injury of operation or wound to human body, traditional suture formula is gradually glued
Mixture is replaced.The conventional adhesive of domestic medical industry has fibrin（Also referred to as fibrin）, cyanoacrylate.Blood
Fibrin is because of its mechanism of action, and its cohesive is weaker, sufferer after use works nursing it is particularly important, but it is careless slightly will
Cause the rupture of wound, the serious secondary injury that can also cause wound, and cyanoacrylate is used as the bonding of chemical
Agent, although its bonding quality is much better than fibrin, its toxic and side effect is larger, and physique slightly sensitive crowd all do not apply to.
Modern study finds that the mucus of giant salamander body surface has extremely strong cohesive, and the hemostasis of second level is bonded, and because it is pure
Natural biological raw material, without any toxic and side effect, so the prospect that giant salamander mucus is used for the fields such as medical operating is extremely wide
It is wealthy.Because giant salamander living environment it is more harsh, it is impossible to the extensive, cultivation that generalizes, giant salamander skin mucus are obtained in addition
Method it is extremely traditional, the mucus of its skin surface could be collected after the giant salamander is slaughtered, the rareness of giant salamander mucus is also limited
Giant salamander mucus is used for adhesive of medical.So finding a kind of a large amount of of giant salamander skin mucus, scale, the collection of normalization
Method is particularly important.
The content of the invention
For solve prior art in can only by slaughtering after the giant salamander collect its skin mucus, collect mucus amount it is few and
Do not possess the technical problem of continuation, what the present invention provided a kind of giant salamander skin mucus artificially collects method, is not slaughtering baby
On the basis of baby fish, can periodicity, live body, a large amount of methods for collecting giant salamander skin mucus.
A kind of giant salamander skin mucus artificially collect method, comprise the following steps：
A, select the giant salamander：Choose not received in scaring and 25 days in more than three and half ages, 25 days and do not taken mucus
The giant salamander；
B, body surface sterilization：Giant salamander body surface is carried out into manual cleaning with light salt brine, giant salamander skin surface is cleaned；
C, artificial energization：The low-voltage of low below 36V is led to giant salamander body surface；
D, wait：After waiting for 50 seconds quietly, treat that giant salamander skin mucus carry out naturally secret accumulation；
E, collection mucus：Mucus is collected in giant salamander body surface, power is slowly increased, but not injure giant salamander body surface organization as original
Then；
F, sterilization：Giant salamander body surface after to taking mucus puts back to culturing pool after carrying out disinfection.
Further, in described step b, the concentration of the light salt brine for being used is 2-3%, if concentration is too low, can shadow
Disinfection Effect is rung, if light salt brine concentration is high, beyond above-mentioned number range, then the medicinal effects of the mucus of collection can be affected.
Further, best results when magnitude of voltage is 30-36V in described step c.
Further, in described step c, electric current is 0.05-0.1 MAHs, and the duration is the 3-6 seconds.If low
In above-mentioned data, the collecting amount of mucus is few, and economic benefit is impacted；And if exceeding above-mentioned numerical value, can be to the body of the giant salamander
Form stealthy injury, collect the giant salamander apocleisis after mucus, it is easily frightened, take after mucus in 15 days, body surface wound easily infects.
So above-mentioned numerical value is to consider several aspects：1st, the maximum of collection mucus, 2, it is impossible to which the normal growth of the giant salamander is made
Into impact, 3, take time of one month after mucus and can not be improved by the probability of trauma infection contamination.And through triennium, 300
Bar giant salamander statistics verifies that above-mentioned data can make completely the giant salamander on the basis of normal growth, collect the maximum amount of
The mucus of body surface secretion, and after being combined with step f, then the mucus collected does not interfere with completely the growth of the giant salamander, collects latter
The trauma infection contamination of individual month, and the quantity of the collectable mucus of giant salamander next month（Statistics is collected by 3 years giant salamanders
Quantity maximum）.
In step f, after collecting mucus, disinfected with the rough gentian gentian violet of less than 1.5% concentration before putting in a suitable place to breed again, sprayed
Or it is coated in giant salamander body surface.
It is of the invention to gather giant salamander skin mucus by artificial, work(can be bonded with the hemostasis of second level using giant salamander mucus
Can, and because it is pure natural biological raw material, without any toxic and side effect, so giant salamander mucus is used for the fields such as medical operating
Prospect it is extremely wide.By the collection and utilization of mucus, skin mucus can also can use as the raw material of high-quality adhesive of medical
The collection method of giant salamander skin mucus adopts artificial stimulation in other medical applications, the present invention, while collection mucus not
The growth of the giant salamander is affected, the added value of doll's fish culture is can greatly improve.
Specific embodiment
In order to more fully explain the enforcement of the present invention, the skill of the present invention is further illustrated below in conjunction with specific embodiment
Art effect.Example is served only for explaining the present invention, rather than limits protection scope of the present invention.
The giant salamander of same batch cultivation is chosen, is divided into two batches（Applicant selects altogether 20 giant salamanders）, wherein a collection of
Secondary collection mucus, in addition a batch do not collect skin mucus, the giant salamander was two ages, and body weight is：2.5 kilograms are added and subtracted 50 grams, with
It is as follows that parameters described below completes result of the test：Giant salamander body surface is carried out into manual cleaning with light salt brine, makes giant salamander skin surface clear
It is clean；Lead to the low-voltage of low 36V to giant salamander body surface, electric current is 0.06 MAH, and the duration is 5 seconds；After waiting for 50 seconds quietly, treat
Giant salamander skin mucus carry out naturally secret accumulation；Mucus is collected in giant salamander body surface, power is slowly increased, but not injure baby
Baby's fish body table organization is principle；Giant salamander body surface after to taking mucus is disinfected with the rough gentian gentian violet of 1% concentration, is carried out
Culturing pool is put back to after sterilization.
Using said method, the giant salamander can collect giant salamander skin mucus 0.4-0.6kg every January.Statistics, Jing
Cross the comparison up to one-year age, body weight aspect, gather always skin mucus the giant salamander with do not gathered skin mucus
The giant salamander is remained basically stable, so gathering skin mucus to the giant salamander itself substantially without impact.
Embodiments of the invention are the foregoing is only, the scope of the claims of the present invention can not be therefore limited, it is every using this
Equivalent, equivalent variations and improvement that content described in patent application file is made, in the scope of patent protection of the present invention.
Claims (5)
1. a kind of giant salamander skin mucus artificially collect method, comprise the following steps：
A, select the giant salamander：Choose not received in scaring and 25 days in more than three and half ages, 25 days and do not taken mucus
The giant salamander；
B, body surface sterilization：Giant salamander body surface is carried out into manual cleaning with light salt brine, giant salamander skin surface is cleaned；
C, artificial energization：The low-voltage of low below 36V is led to giant salamander body surface；
D, wait：After waiting for 50 seconds quietly, treat that giant salamander skin mucus carry out naturally secret accumulation；
E, collection mucus：Mucus is collected in giant salamander body surface, power is slowly increased, but not injure giant salamander body surface organization as original
Then；
F, sterilization：Giant salamander body surface after to taking mucus puts back to culturing pool after carrying out disinfection.
2. a kind of giant salamander skin mucus according to claim 1 artificially collect method, it is characterised in that：Described step
In rapid b, the concentration of the light salt brine for being used is 2-3%.
3. a kind of giant salamander skin mucus according to claim 1 artificially collect method, it is characterised in that：Described step
Best results when magnitude of voltage is 30-36V in rapid c.
4. a kind of giant salamander skin mucus according to claim 1 artificially collect method, it is characterised in that：In step f,
Disinfected with the rough gentian gentian violet of less than 1.5% concentration before putting in a suitable place to breed again, spray or be coated in giant salamander body surface.
5. method is artificially collected according to a kind of claim 1-4 arbitrary described giant salamander skin mucus, it is characterised in that：Institute
In the step of stating c, electric current is 0.05-0.1 MAHs, and the duration is the 3-6 seconds.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611231165.5A CN106619739B (en) | 2016-12-28 | 2016-12-28 | Artificial collection method of giant salamander surface mucus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611231165.5A CN106619739B (en) | 2016-12-28 | 2016-12-28 | Artificial collection method of giant salamander surface mucus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106619739A true CN106619739A (en) | 2017-05-10 |
| CN106619739B CN106619739B (en) | 2020-06-30 |
Family
ID=58831813
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611231165.5A Active CN106619739B (en) | 2016-12-28 | 2016-12-28 | Artificial collection method of giant salamander surface mucus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106619739B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109601437A (en) * | 2019-01-25 | 2019-04-12 | 淮海工学院 | The method that Mucilage of loach stress is secreted and collected |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104223121A (en) * | 2014-09-16 | 2014-12-24 | 重庆馗旭生物科技股份有限公司 | Extracting device for giant salamander skin mucus and extracting method |
| CN104542454A (en) * | 2014-10-24 | 2015-04-29 | 重庆翰锋农业发展有限公司 | Giant salamanders artificial breeding technology |
-
2016
- 2016-12-28 CN CN201611231165.5A patent/CN106619739B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104223121A (en) * | 2014-09-16 | 2014-12-24 | 重庆馗旭生物科技股份有限公司 | Extracting device for giant salamander skin mucus and extracting method |
| CN104542454A (en) * | 2014-10-24 | 2015-04-29 | 重庆翰锋农业发展有限公司 | Giant salamanders artificial breeding technology |
Non-Patent Citations (1)
| Title |
|---|
| 许永杰等: "成年大鲵实验室饲养及蜕皮和体表粘液收集方法", 《遵义医学院学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109601437A (en) * | 2019-01-25 | 2019-04-12 | 淮海工学院 | The method that Mucilage of loach stress is secreted and collected |
| CN109601437B (en) * | 2019-01-25 | 2021-06-15 | 淮海工学院 | Method for stress secretion and collection of loach mucus |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106619739B (en) | 2020-06-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Baer | The treatment of chronic osteomyelitis with the maggot (larva of the blow fly) | |
| KR101670953B1 (en) | Manufacturing method for sterilization deodorant and sterilization deodorant thereof | |
| CN105684996B (en) | A kind of medical grade hirudinaria manillensis cultural method | |
| JP6282287B2 (en) | Pharmaceutical composition for treating thermal injury and wound with bone injury | |
| CN104069541A (en) | Preparation method of collagen suture line | |
| EP3932436A1 (en) | Medical adhesive, and manufacturing method therefor and use thereof | |
| CN107349220A (en) | A kind of preparation comprising fibroblast excretion body and application thereof | |
| CN104474573A (en) | Biological mask and preparation method thereof | |
| CN106619739A (en) | Artificial collection method of body surface mucus of giant salamander | |
| CN102067892B (en) | Anticorrosive Chinese medicine preparation and application thereof | |
| CN104815096B (en) | A kind of nanometer composite skin disinfection emulsion and preparation method thereof | |
| CN111973637A (en) | Method for treating onychomycosis by using probiotic liquid | |
| CN104548192A (en) | Preparation method of collagen suture lines | |
| TWI288646B (en) | Novel dressing materials capable of promoting rapid healing of wounded skin surfaces | |
| CN106421887A (en) | Medical dressing | |
| CN206324056U (en) | A kind of giant salamander skin mucus special collector | |
| US10118033B2 (en) | System and method for reducing inflammation of tissue | |
| RU2337704C1 (en) | Method of postsurgery rehabilitation of fishes | |
| US20100099758A1 (en) | Regenerative wound healing using copper-silver citrate composition | |
| CN107308340A (en) | It is a kind of to treat pharmaceutical composition of wound and preparation method thereof | |
| CN107509987B (en) | Preparation method of fish skin with biological activity and without scale pocket | |
| CA2752245C (en) | Implants made of the inner core of drupes and/or palm tree fruits | |
| TWI623261B (en) | Method for producing sterile fly eggs | |
| Khan | Study in diseases of fish: infestation of fish with leeches and fish lice | |
| CN107347928B (en) | In-vitro protection liquid for brucellosis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20221209 Address after: 277700 Second floor of New Times Building, Lanling County Economic Development Zone, Linyi City, Shandong Province Patentee after: Lanling Finance Industry Development Co.,Ltd. Address before: 561208 Shuitangzhai, Chengguan Town, Zhenning Buyi Miao Autonomous County, Anshun City, Guizhou Province Patentee before: Anshun Sihai Agricultural Science & Technology Development Co.,Ltd. |