CN104815096B - A kind of nanometer composite skin disinfection emulsion and preparation method thereof - Google Patents
A kind of nanometer composite skin disinfection emulsion and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of nanometer composite skin disinfection emulsions and preparation method thereof.The nanometer composite skin disinfection emulsion is made of double-chain quaternary ammonium salt class compound, inorganic nano antiseptic substance, emulsifier, extract solution from aloe and water；The double-chain quaternary ammonium salt class compound is double decyl dimethyl ammonium bromides, double-track suspended self-propelled conveyor or bis- (dodecyl dimethyl) the ethylene diammoniums of bromination；The inorganic nano antiseptic substance is nano zine oxide, nano phase ag_2 o or nano-titanium dioxide；The extract solution from aloe is aloe stoste.Multiple emulsion of the present invention acts on 1min to staphylococcus aureus, Escherichia coli, Candida albicans, and killing rate reaches 100%；To 6h, 12h of natural bacteria, for 24 hours, the inhibiting rate of 48h it is high；The lotion acts on 1min to subject's hand, is averaged killing rate greater than 90% to natural bacteria；It is with good stability；It can be used safely on human skin, mucous membrane, and stimulation non-hazardous to environment and human body.
Description
Technical field
The present invention relates to a kind of disinfection emulsion and preparation method thereof more particularly to a kind of nanometer composite skin disinfection emulsion and
Preparation method.
Background technique
Skin and mucous membrane are the effective barriers that human body resists that microorganism invades profit tissue, but have micro- life abundant on its surface
Object.In daily life, by opponent and mucocutaneous disinfection, enteric infectious disease, skin pyesis and uropoiesis can be prevented
The infection of reproductive system；In health system, the important leverage that skin degerming is modern medical service, and control hospital are carried out
Infect one of most important, most easy-to-use measure.
Currently used skin and mucous membrane disinfection agent huge number, but more or less defect is suffered from, as quaternary ammonium salt is anti-
Bacterium spectrum is narrow, and sterilizing ability is weak；Ethyl alcohol is big to skin and mucous membrane irritation；The Iodophor bactericidal effect duration is shorter；Chlorhexidine acetate
Class is also easy to produce antibody-resistant bacterium.Traditional disinfection agent is difficult to comply fully with the needs of clinical departments medical staff's hand disinfection, to make
At cross-infection.Therefore, it is necessary to find New raxa, novel form or new compound prescription, to reach ideal skin degerming effect.
Summary of the invention
The object of the present invention is to provide a kind of nanometer composite skin disinfection emulsion and preparation method thereof, the skin disinfection emulsions
By double-chain quaternary ammonium salt compound and inorganic nano antiseptic substance compatibility, and emulsifier and aloe extract is added, had both played
Machine class antibacterial agent bactericidal effect is good, fireballing feature, but can make formula obtain inorganic antiseptic has a broad antifungal spectrum, lasting effect it is good,
It is heat-resist, be not likely to produce the features such as drug resistance, can effectively kill the staphylococcus aureus of skin surface, Escherichia coli and white
Color candida albicans.
Nanometer composite skin disinfection emulsion provided by the invention, it is by double-chain quaternary ammonium salt class compound, inorganic nano antiseptic
Substance, emulsifier, extract solution from aloe and water composition；
The double-chain quaternary ammonium salt class compound is that double decyl dimethyl ammonium bromides, double-track suspended self-propelled conveyor or bromination are double
(dodecyl dimethyl) ethylene diammonium；
The inorganic nano antiseptic substance is nano zine oxide, nano phase ag_2 o or nano-titanium dioxide；
The extract solution from aloe is aloe stoste.
In above-mentioned nanometer composite skin disinfection emulsion, the emulsifier is by three normal-butyl phosphate (tributyl phosphate), mountain
Pears alcohol acid anhydride monopalmitate (sorbitan monopalmitate, Sorbitan monopalmitate；Trade name span 40 or
This disk 40, Span 40, CAS accession number: 26266-57-9) and Tween-80
(Polyoxyethylenesorbitan monooleate；Sorbitan monooleate ethoxylate；Trade name is spat
Temperature 80, Tween 80, CAS accession number: 9005-65-6) composition, volume ratio is 1:(2~3): (1~3), concretely 1:2:2.
In above-mentioned nanometer composite skin disinfection emulsion:
The mass-volume concentration of the double-chain quaternary ammonium salt class compound is 1~5g/L；
The mass-volume concentration of the inorganic nano antiseptic substance is 0.1~0.5g/L, and partial size is 50~100nm；
The volume fraction of the emulsifier is 10%~20%；
The volume fraction of the extract solution from aloe is 20%~30%；
Surplus is water.
In above-mentioned nanometer composite skin disinfection emulsion, the extract solution from aloe can be aloe stoste, and aloe stoste is reed
The normal juice of luxuriant growth can be specifically commercially available by commercial sources, can also be prepared via a method which to obtain: mistake after aloe is squeezed the juice
Filter, taking filtrate is aloe stoste.
In above-mentioned nanometer composite skin disinfection emulsion, the double-chain quaternary ammonium salt class compound have toxicity it is low, it is nonirritant,
Without bad smell, can be the disinfection and sterilization under hard water conditions the features such as；But traditional double-chain quaternary ammonium salt class compound disinfectant belongs to
Low effect disinfectants, Germicidal Efficacy stronger for drag is poor, and is better than leather Lan Yang to gram-negative bacteria killing effect
Property bacterium；
The mass-volume concentration of the double-chain quaternary ammonium salt class compound can be 1~5g/L, concretely 1~2g/L, 2~
5g/L, 1g/L, 2g/L or 5g/L, preferably 1g/L；
In above-mentioned nanometer composite skin disinfection emulsion, the inorganic nano antiseptic substance is preferably nano zine oxide, described
Nano zine oxide has good anti-microbial property and chemical compatibility.Its Antibacterial Mechanism is main are as follows: (1) ZnOw and particle
Reach nanoscale, thus the special skin effect and high oxidation activity having with nano material；(2) zinc oxide is a kind of
Semiconductor material, wherein there is a large amount of elemental oxygens to release；(3) the unique four needle-shaped network structures that are formed by of ZnOw are
Antibacterial ion provides high efficiency of transmission path, when negatively charged bacterium touches any position of network, since positive and negative charge is inhaled
Gravitation, a large amount of zinc ions are moved through to come with the speed of electric current, and penetrating cell wall destroys the synthesis of thallus internal protein
(Zuowan Zhou,Shikai Liu,Lixia Gu.Studies on the strength and wear resistance
of tetratod-shaped ZnO whisker-reinforced rubber composites.[J]Applied
Polymer Science,2001,80:1520-24)；
The mass-volume concentration of the inorganic nano antiseptic substance can be 0.1~0.5g/L, concretely 0.1g/L or
0.5g/L；Partial size can be 50~100nm.
In above-mentioned nanometer composite skin disinfection emulsion, the volume fraction of the emulsifier can be 10%~20%, specifically may be used
It is 10% or 20%.
In above-mentioned nanometer composite skin disinfection emulsion, the extract solution from aloe has inhibiting effect to multiple-microorganism, grinds
Studying carefully display, it is slightly above Gram-negative bacteria to the antibacterial effect of gram-positive bacteria, and main function principle may be thin to bacterium
The peptide glycan of cell wall has an impact, and destroys the cell wall of bacterium and achievees the purpose that bacterium；
The volume fraction of the extract solution from aloe can be 20%~30%, concretely 20% or 30%.
Double-chain quaternary ammonium salt compound and extract solution from aloe use in conjunction are not only enhanced its antibacterial ability by the present invention, but also are increased
Add its antimicrobial spectrum, and imparts the protective effect being formulated to skin；Meanwhile combining inorganic nano antiseptic substance with them,
Compound prescription antibacterial effect can be enhanced, and assign the nanometer composite skin disinfection emulsion new holding effect antibacterial effect, the association of three
Make nanometer composite skin disinfection emulsion of the present invention that there is good sterilizing function with synergistic effect, it is contemplated that the toxicity of disinfectant
It should control in zone of reasonableness, the double-chain quaternary ammonium salt content highest that the present invention uses is limited to 5g/L, and zinc oxide is controlled in 0.5g/L,
Extract solution from aloe is 30%.
In above-mentioned nanometer composite skin disinfection emulsion, the nanometer composite skin disinfection emulsion concretely following 1) -7)
In it is any:
1) 1~5g/L of double-chain quaternary ammonium salt class compound quality volumetric concentration, inorganic nano antiseptic material mass volumetric concentration
0.1~0.5g/L, emulsifier volume fraction 10%~20%, extract solution from aloe volume fraction 20%~30%, solvent are water；
2) 1~2g/L of double-chain quaternary ammonium salt class compound quality volumetric concentration, inorganic nano antiseptic material mass volumetric concentration
0.1~0.5g/L, emulsifier volume fraction 10%~20%, extract solution from aloe volume fraction 20%~30%, solvent are water；
3) 2~5g/L of double-chain quaternary ammonium salt class compound quality volumetric concentration, inorganic nano antiseptic material mass volumetric concentration
0.1~0.5g/L, emulsifier volume fraction 10%~20%, extract solution from aloe volume fraction 20%~30%, solvent are water；
4) double-chain quaternary ammonium salt class compound quality volumetric concentration 1g/L, inorganic nano antiseptic material mass volumetric concentration
0.5g/L, emulsifier volume fraction 10%, extract solution from aloe volume fraction 30%, solvent are water；
5) double-chain quaternary ammonium salt class compound quality volumetric concentration 2g/L, inorganic nano antiseptic material mass volumetric concentration
0.5g/L, emulsifier volume fraction 20%, extract solution from aloe volume fraction 20%, solvent are water；
6) double-chain quaternary ammonium salt class compound quality volumetric concentration 5g/L, inorganic nano antiseptic material mass volumetric concentration
0.1g/L, emulsifier volume fraction 10%, extract solution from aloe volume fraction 20%, solvent are water；
7) double-chain quaternary ammonium salt class compound quality volumetric concentration 1g/L, inorganic nano antiseptic material mass volumetric concentration
0.5g/L, emulsifier volume fraction 20%, extract solution from aloe volume fraction 30%, solvent are water.
Nanometer composite skin disinfection emulsion of the present invention is to extract double-chain quaternary ammonium salt compound, nano zine oxide and aloe
A kind of disinfectant that liquid is used in combination, and a kind of oil-in-water emulsion structure is formed, the diameter of drop is 10 in the disinfectant
~30nm.
Invention further provides a kind of preparation methods of above-mentioned nanometer composite skin disinfection emulsion, including walk as follows
It is rapid: by above-mentioned nanometer composite skin disinfection emulsion each component mix after, through it is high-pressure homogeneous can be obtained it is described nano combined
Skin disinfection emulsion.
In the preparation method of above-mentioned nanometer composite skin disinfection emulsion, according to the following concentration of each component in final products into
Row mixing:
The mass-volume concentration of the double-chain quaternary ammonium salt class compound is 1~5g/L；The inorganic nano antiseptic substance
Mass-volume concentration is 0.1~0.5g/L；The volume fraction of the emulsifier is 10%~20%；The body of the extract solution from aloe
Fraction is 20%~30%.
The specific steps of above-mentioned preparation method are as follows: weigh (measurement) a certain amount of double-chain quaternary ammonium salt compound, aloe is extracted
Liquid is separately added into the beaker for filling distilled water, blender stirring is set, until being thoroughly mixed dissolution.Measure a certain amount of composite emulsifying
Agent is added in above-mentioned mixed solution, stirs evenly.A certain amount of nano zine oxide is weighed in proportion, is added in mixed solution, is set and stir
It mixes device after mixing evenly, is emulsified through high pressure homogenizer.
In above-mentioned preparation method, it is described it is high-pressure homogeneous at 25~35Mpa, 20~30 DEG C under conditions of carry out, pressure tool
Body can be 25~30Mpa, 30~35Mpa, 20Mpa, 30Mpa or 35Mpa, concretely 20 ± 2 DEG C of temperature.
Preparation method of the present invention through high-pressure homogeneous emulsion process, mixes above-mentioned formula with magnetic force mixing method and hypervelocity cutting machine
It is legal to compare, not only there are stirring, shearing, high-speed impact effect, it is also comprehensive using liquid decompression, expansion, explosion under condition of high voltage etc.
Effect is closed, the lotion obtained using the feature is not only uniformly dispersed, and it is not stratified, and relatively fine particle is obtained, reach preferable emulsification
Effect, therefore, the formula still have preferable bactericidal property while reducing double-chain quaternary ammonium salt content.
Present invention provides above-mentioned nanometer composite skin disinfection emulsions in skin degerming or to prepare in disinfectant liquid for skin
Using.
In above-mentioned application, the disinfectant liquid for skin can kill staphylococcus aureus, Escherichia coli and Candida albicans.
The invention has the following beneficial effects:
The formula of nanometer composite skin disinfection agent of the present invention is free of alcohols, and to mucocutaneous nontoxic non-stimulated, use is safe
Property is preferable.Emulsion liquid not only makes formula have good appearance, also adds its stability.
Experiments have shown that:
(1) multiple emulsion of the present invention acts on 1min, killing rate to staphylococcus aureus, Escherichia coli, Candida albicans
Reach 100%；The simple double-chain quaternary ammonium salt solution or double-chain quaternary ammonium salt for being significantly better than same amount add extract solution from aloe, inorganic
Nano-antibacterial substance solution.
(2) simulation field experiment shows, nanometer composite skin disinfection emulsion to 6h, 12h of natural bacteria, for 24 hours, the suppression of 48h
Rate processed is significantly higher than simple double-chain quaternary ammonium salt aqueous solution with same amount and double-chain quaternary ammonium salt adds extract solution from aloe compound
Object.
(3) skin on-the-spot disinfection experimental result is shown, which acts on 1min to subject's hand, is averagely killed to natural bacteria
Rate of going out is greater than 90%.
(4) high temperature accelerated test is shown, lotion active constituent content rate of descent after 54 DEG C of storage 14d is 3.05%,
It is with good stability.
(5) toxicity test shows that the lotion can be used safely on human skin, mucous membrane, it is non-hazardous to environment and human body and
Stimulation.
Specific embodiment
Experimental method used in following embodiments is conventional method unless otherwise specified.
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
Extract solution from aloe in following embodiments is prepared via a method which to obtain:
Fresh aloe is taken, after being sterilized with the potassium permanganate that mass fraction is 0.11%, is rinsed well with circulating water, with juicing
Machine is squeezed out absinthe-green juice, with 5 layers of filtered through gauze.
Embodiment 1 prepares nanometer composite skin disinfection emulsion
Nanometer composite skin disinfection emulsion is prepared as steps described below:
0.1g double-track suspended self-propelled conveyor is added in the beaker for filling 40~60mL distilled water, blender stirring is set,
To being completely dissolved.30mL extract solution from aloe is slowly added into double-track suspended self-propelled conveyor solution, it is stirring while adding.Suction pipe is inhaled
10mL compound emulsifying agent (containing three normal-butyl phosphate 2mL, span 40 4mL, Tween 80 4mL) is taken to be added in above-mentioned mixed solution,
It stirs evenly.0.05g is weighed in proportion, the nano zine oxide that partial size is 50~100nm, be added in mixed solution, distillation is added
Solution is supplied 100mL postposition blender after mixing evenly by water, through high pressure homogenizer in room temperature (20 DEG C ± 2 DEG C), 25Mpa work
Pressure condition is emulsified to form milky opaque emulsion.
In the present embodiment, each component is final concentration of in the disinfection emulsion prepared by mentioned component with method: double decyls two
Ammonio methacrylate 1g/L, extract solution from aloe 30% (v/v), nano zine oxide 0.5g/L, emulsifier account for the disinfection emulsion total volume
10%.The diameter of drop is 10~30nm in the lotion, has reached Nano grade.
Embodiment 2 prepares nanometer composite skin disinfection emulsion
Nanometer composite skin disinfection emulsion is prepared as steps described below:
The bis- decyl dimethyl ammonium bromides of 0.2g are added in the beaker for filling 40~60mL distilled water, blender stirring is set,
To being completely dissolved.20mL extract solution from aloe is slowly added into double decyl dimethyl ammonium bromide solutions, it is stirring while adding.Suction pipe is inhaled
20mL compound emulsifying agent (containing three normal-butyl phosphate 4mL, span 40 8mL, Tween 80 8mL) is taken to be added in above-mentioned mixed solution,
It stirs evenly.0.05g is weighed in proportion, the nano zine oxide that partial size is 50~100nm, be added in mixed solution, distillation is added
Solution is supplied 100mL postposition blender after mixing evenly by water, through high pressure homogenizer in room temperature (20 DEG C ± 2 DEG C), 35Mpa work
Pressure condition is emulsified to form milky opaque emulsion.
In the present embodiment, each component is final concentration of in the disinfection emulsion prepared by mentioned component with method: double decyls two
Methyl bromide ammonium 2g/L, extract solution from aloe 20% (v/v), nano zine oxide 0.5g/L, emulsifier account for the disinfection emulsion total volume
20%.The diameter of drop is 10~30nm in the lotion, has reached Nano grade.
Embodiment 3 prepares nanometer composite skin disinfection emulsion
Bis- (dodecyl dimethyl) the ethylene diammoniums of 0.5g bromination are added in the beaker for filling 40~60mL distilled water, are set
Blender stirring, until being completely dissolved.20mL extract solution from aloe is slowly added into bis- (dodecyl dimethyl) the ethylene diammoniums of bromination
It is stirring while adding in solution.Suction pipe draws 10mL compound emulsifying agent and (contains three normal-butyl phosphate 2mL, span 40 4mL, tween
It 804mL) is added in above-mentioned mixed solution, stirs evenly.0.01g is weighed in proportion, partial size is the nano oxidized of 50~100nm
Zinc is added in mixed solution, and distilled water is added by solution and supplies 100mL postposition blender after mixing evenly, through high pressure homogenizer
At room temperature (20 DEG C ± 2 DEG C), 30Mpa operating pressure conditions are emulsified to form milky opaque emulsion.
In the present embodiment, each component is final concentration of in the disinfection emulsion prepared by mentioned component with method: bromination bis- (ten
Dialkyl dimethyl) ethylene diammonium 5g/L, extract solution from aloe 20% (v/v), nano zine oxide 0.1g/L, emulsifier accounts for the disinfection
The 10% of total emulsion volume.The diameter of drop is 10~30nm in the lotion, has reached Nano grade.
Embodiment 4 prepares nanometer composite skin disinfection emulsion
0.1g double-track suspended self-propelled conveyor is added in the beaker for filling 40~50mL distilled water, blender stirring is set,
To being completely dissolved.30mL extract solution from aloe is slowly added into double decyl dimethyl ammonium bromide solutions, it is stirring while adding.Suction pipe is inhaled
20ml compound emulsifying agent (containing three normal-butyl phosphate 4mL, span 40 8mL, Tween 80 8mL) is taken to be added in above-mentioned mixed solution,
It stirs evenly.0.05g is weighed in proportion, the nano zine oxide that partial size is 50~100nm, be added in mixed solution, distillation is added
Solution is supplied 100mL postposition blender after mixing evenly by water, through high pressure homogenizer in room temperature (20 DEG C ± 2 DEG C), 30Mpa work
Pressure condition is emulsified to form milky opaque emulsion.
In the present embodiment, each component is final concentration of in the disinfection emulsion prepared by mentioned component with method: double decyls two
Ammonio methacrylate 1g/L, extract solution from aloe 30% (v/v), nano zine oxide 0.5g/L, emulsifier account for the disinfection emulsion total volume
20%.The diameter of drop is 10~30nm in the lotion, has reached Nano grade.
The performance test of embodiment 5, nanometer composite skin disinfection emulsion
1, physicochemical property
After tested, above-described embodiment 1-4 prepares resulting disinfection emulsion and is all satisfied following physicochemical characters:
Appearance: milky is opaque
PH value: 8.10
Particle diameter (nm): 10~30nm under Electronic Speculum
Stability: using high temperature accelerated test, which places 14 days bactericidal active ingredient rates of descent in 54 DEG C of insulating boxs
≤ 10%, it is with good stability.
2, bactericidal effect
The sterilization test of nanometer composite skin disinfection emulsion is (test is carried out referring to " disinfection technology standard ") as follows,
Acquired results are the preparation gained nanometer composite skin disinfection emulsion test result of embodiment 4 unless otherwise instructed.
Neutralizer in following tests is glycine containing 2g/L, 3g/L lecithin, 2.5% Tween 80 and 10% small ox blood
Clear phosphate buffer, can complete neutralization skin disinfection emulsion stoste to staphylococcus aureus, Escherichia coli and white read
The killing effect of coccus, and on the ingredient of culture medium without influence, meet and is required as defined in " disinfection technology standard ".
(1) different formulations composition compares the carrier quantitative germicidal test result of different microorganisms
Solution A formulation: double-track suspended self-propelled conveyor 1g/L, extract solution from aloe 30%, nano zine oxide 0.5g/L, emulsification
Agent 20%.
Solution B formula: double-track suspended self-propelled conveyor 1g/L.
Solution C formula: double-track suspended self-propelled conveyor 1g/L, extract solution from aloe 30%.
Solution D formula: double-track suspended self-propelled conveyor 1g/L, extract solution from aloe 30%, nano zine oxide 0.5g/L.
Carrier is carried out to solution A, B, C and D and impregnates quantitative disinfecting test, and experimental bacteria is respectively staphylococcus aureus
(ATCC6538), Escherichia coli (8099) or Candida albicans (ATCC 10231).By the dosage of a piece of bacterium piece 5.0mL, by A,
B, tetra- kinds of formula solution stostes of C, D (positive control is phosphate buffer PBS) are added in sterilized petri dishes.Set 19~21 DEG C of water-baths
Upper preheating 5min, adds bacterium piece.Certain time is acted on, bacterium piece is moved into the test tube equipped with 5.0mL neutralizer, neutralization
10min.It beats test tube 80 times, to elute bacterium on piece bacterium and mix.It takes eluent 5.0mL to do count plate, calculates killing rate.Than
Compared with four to the killing effect of microorganism, acquired results are as shown in table 1.
Table 1, different formulations performance comparison result
Note: test temperature is 19~21 DEG C.Positive controls are averaged bacterium number are as follows: staphylococcus aureus is
1350000cfu/ piece, Escherichia coli are 3400000cfu/ piece, and Candida albicans is 3200000cfu/ piece.
The nano combined disinfection emulsion stoste of the present invention is to staphylococcus aureus, large intestine bar it can be seen from upper table result
The average killing rate of bacterium and Candida albicans reaches 100%, and stability is good, therefore, double-chain quaternary ammonium salt compound and aloe
Extracting solution, nano zine oxide and emulsifier compatibility have obviously staphylococcus aureus, Escherichia coli, Candida albicans
Synergistic function, bactericidal effect is significantly improved.
(2) to the comparison of natural bacteria holding effect antibacterial ability
The spare silicone tube that will sterilize is soaked in respectively containing double-track suspended self-propelled conveyor 1g/L, extract solution from aloe
In the nano combined disinfection emulsion stoste (solution A) that 30% aqueous solution (solution C) and the present invention is prepared, it is completely soaked
It takes out and dries after 3 minutes.As positive control after separately taking untreated silicone tube to sterilize.Above-mentioned silicone tube is taken to set same plate
It is interior, put 4 plates altogether, opening sets Microbiological Lab's windowsill, in 6h, 12h, for 24 hours, 48h taken out with aseptic nipper, be put into containing
In the test tube of 5mL neutralizer, vibration stands 10min after striking, and observes result after 48~72h of inoculated and cultured.Test result is shown in Table 2.
Table 2, different disposal group place different time to the growth inhibition situation of natural bacteria
Nanometer composite skin disinfection emulsion of the present invention is strong to the rejection ability of natural bacteria it can be seen from 2 result of table, and double
Chain quaternary ammonium salt adds aloe formula of liquid to compare, and the compound prescription of addition inorganic nano antiseptic substance and emulsifier is in 6h-48h to nature
The holding effect antibacterial ability of bacterium is significantly increased.
(3) hand site test results
After the mutually abundant rubbing of subject's both hands, left hand is sampled with cotton examination semar technique, as not sterilizing control group.So
Afterwards, stoste 3mL is smeared in the right hand uniform.Effect 2 minutes adopts disinfection group sample to right hand smearing.When sampling, 5 fingers close up, by nothing
Bacterium cotton swab is soaked in invisible spectro 5mL neutralizer, after extracting on tube wall, is referring to curved surface from finger tip to finger with round-trip
It embrocates 2 times, often embrocates one time, cotton swab is rotated primary.In the test tube that cotton swab head is cut to dress neutralizer.Neutralization 10 divides
Clock beats test tube to elute bacterium in cotton swab and mix.It takes eluent to do count plate, calculates killing rate.Test result is shown, is received
Rice disinfection emulsion act on 1 minute, to 30 person-times on hand natural bacteria average killing rate be 95.09%.
3, toxicological test:
It is tested by " disinfection technology standard " (2002 editions) specific requirement, acquired results are reality unless otherwise instructed
Apply the test result of the preparation gained nanometer composite skin disinfection emulsion of example 4.
(1) acute oral toxicity test
The Switzerland that weight is 18~22g is selected to plant Kunming outbreeding system healthy mice 20 (female, male fifty-fifty), if a dosage
Group 6000mg/kg, after empty stomach 16h hours, by the disposably oral stomach-filling of 0.2mL/10g weight.After stomach-filling, it is observed continuously 14 days
Animal poisoning performance and death toll, calculate LD50.The results show that nanometer disinfection emulsion tests LD to chmice acute Oral toxicity50>
5000mg/kg weight belongs to practical nontoxic grade.
(2) Skin Irritation Test
Selecting weight is rabbit 4 of 2.2~3.0kg, and male and female are unlimited.Before test for 24 hours, the hair of back part of animal two sides is cut
Fall, range 3cm2.Next day is by tested material stoste 0.2mL drop in 2.5cm2On 2 layers of gauze of size and apply in side skin table
Then face is covered with one layer of non-stimulated oilpaper, then with non-stimulated immobilization with adhesive tape.The other side is as blank control (or solvent pair
According to).4h is acted on, then washes away residual tested material with warm water；After removing tested material 1h, for 24 hours, 48h, observe local skin
Reaction, scores.The results show that the lotion acts on a rabbit skin 4h, animal skin stimulate the reaction, skin irritatin are not caused
Index < 0.4, stimulus intensity belong to nonirritant.
(3) eye irritant test
Selecting weight is rabbit 4 of 2.0~3.0, and male and female are unlimited.Stoste 1-2 drop is taken, rabbit side eye conjunctiva capsule is instilled
Interior, another branch hole is as normal control；Eye is passively closed 4s after drop tested material, with normal saline flushing 5min.After eye drip 1h,
For 24 hours, 48h, 4d, 7d detect by an unaided eye rabbit eye conjunctiva, iris and cornea damage and recovery situation.The results show that the lotion
It is up to 0 to Rabbits with Acute eye irritation score index, stimulus intensity belongs to nonirritant.
(4) micronucleus test
Select Switzerland's kind Kunming outbred mice 50 (female, male fifty-fifty), weight 25-30g.Female, male mouse is divided at random in 5 groups
In.According to the acute toxicity tests, 4 test groups press weight 5000mg/kg, 2500mg/kg, 1000mg/kg agent respectively
The tested material stoste stomach-filling of amount, negative control distilled water stomach-filling, positive control cyclophosphamide (40mg/kg) stomach-filling.Stomach-filling 2
It is secondary, it is spaced 24 hours, 6h draws materials after the 2nd stomach-filling, conventional film-making microscopy.Statistical procedures are carried out with U inspection.The results show that
The lotion is to mouse bone marrow polychromatic erythrocytes without cause micronucleus effect.
The toxicity test the results show that nanometer composite skin disinfection emulsion provided by the invention is safe and nontoxic, non-stimulated,
It can be used safely in human skin, mucous membrane multipath and there is protective effect.
Claims (6)
1. a kind of nanometer composite skin disinfection emulsion, it is by double-chain quaternary ammonium salt class compound, inorganic nano antiseptic substance, emulsification
Agent, extract solution from aloe and water composition；
The double-chain quaternary ammonium salt class compound is double decyl dimethyl ammonium bromides, double-track suspended self-propelled conveyor or bromination bis- (ten
Dialkyl dimethyl) ethylene diammonium；
The inorganic nano antiseptic substance is nano zine oxide, nano phase ag_2 o or nano-titanium dioxide；
The extract solution from aloe is aloe stoste；
The emulsifier is by three normal-butyl phosphate, sorbitol anhydride monopalmitate and Tween-80 group
At volume ratio is 1:(2~3): (1~3)；
In the nanometer composite skin disinfection emulsion:
The mass-volume concentration of the double-chain quaternary ammonium salt class compound is 1~5g/L；
The mass-volume concentration of the inorganic nano antiseptic substance is 0.1~0.5g/L, and partial size is 50~100nm；
The volume fraction of the emulsifier is 10%~20%；
The volume fraction of the extract solution from aloe is 20%~30%；
Surplus is water.
2. nano combined disinfection emulsion according to claim 1, it is characterised in that: in the nano combined disinfection emulsion,
The diameter of drop is 10~30nm.
3. a kind of preparation method of nanometer composite skin disinfection emulsion of any of claims 1 or 2 includes the following steps: to weigh
After benefit requires 1 or 2 each component mixing, the nanometer composite skin disinfection emulsion can be obtained through high-pressure homogeneous.
4. preparation method according to claim 3, it is characterised in that: it is described high-pressure homogeneous in 25~35Mpa, 20~30 DEG C
Under conditions of carry out.
5. nanometer composite skin disinfection emulsion as claimed in claim 1 or 2 is preparing the application in disinfectant liquid for skin.
6. application according to claim 5, it is characterised in that: the disinfectant liquid for skin is to kill Staphylococcus aureus
Bacterium, Escherichia coli and Candida albicans.
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| CN114767712B (en) * | 2022-04-25 | 2023-04-11 | 杭州西子卫生消毒药械有限公司 | Low-volatility disinfectant and preparation method thereof |
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| CN1522145A (en) * | 2001-05-01 | 2004-08-18 | ����ڶ�-PPC�ɷ�����˾ | Compositions and methods for treating vulvovaginitis and vaginosis |
| CN102149400A (en) * | 2008-07-10 | 2011-08-10 | 亚利奥斯制药公司 | Compositions that aim to promote the development and growth of a beneficial vaginal microflora |
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| CN1522145A (en) * | 2001-05-01 | 2004-08-18 | ����ڶ�-PPC�ɷ�����˾ | Compositions and methods for treating vulvovaginitis and vaginosis |
| CN102149400A (en) * | 2008-07-10 | 2011-08-10 | 亚利奥斯制药公司 | Compositions that aim to promote the development and growth of a beneficial vaginal microflora |
| CN103110665A (en) * | 2013-01-24 | 2013-05-22 | 量子高科(中国)生物股份有限公司 | Composition for maintaining genital tract micro-ecological barrier and application thereof |
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