CN106538109A - A kind of method of the efficient fast-germination of Corylus heterophylla Fisch seed - Google Patents
A kind of method of the efficient fast-germination of Corylus heterophylla Fisch seed Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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Abstract
The invention discloses a kind of method of the efficient fast-germination of Corylus heterophylla Fisch seed, comprises the following steps:(1)Seed is plucked;(2)Water seed soaking process;(3)Seed disinfection after seed soaking is processed;(4)Wash seeds after sterilization are clean, and carry out accelerating germination process with phytohormone;(5)Seed after HORMONE TREATMENT is sowed in the hole tray equipped with accelerating germination substrate;(6)20 ~ 25 DEG C of accelerating germination ambient temperature, humidity 70 ~ 75%;(7)Every 5 ~ 6 days to sowing hole tray water spray;(8)The method can make Corylus heterophylla Fisch seed germinate at after planting 10 ~ 12 days, and 30 days seed germination rates grow most fast seedling plant at 30 days or so up to more than 35%, and Herb overall length is up to 30cm, and main root physically well develops, and fibrous root is flourishing;Compare traditional cool stratification method for culturing seedlings, the method can significantly shorten the cultivation time needed for Corylus heterophylla Fisch seed is sprouted, and have the advantages that to emerge efficiency high, growth of seedling quickly, simple operating steps.
Description
Technical field
The present invention relates to biological technical field, more particularly to a kind of the efficient quick of economic forest nut seeds Corylus heterophylla Fisch seed
Germination method.
Background technology
Corylus heterophylla Fisch(Corylus heterophyllaFisch)(Chinese Plants will, Plants of Beijing will are recorded), also known as Corylus heterophylla Fisch
Son, hazel, Semen coryli heterophyllae, be the perennial machaka of Betulaceae Corylus to dungarunga, 3 ~ 5 m of the general height of tree.One of four big nut of the world,
There is the laudatory title of " king of nut ", have an information, Corylus heterophylla Fisch is listed in " delicacy from mountain ", clear because of its kernel taste delicious and crisp, nutritious
Court just becomes tribute as specialties of the Northeast.Corylus heterophylla Fisch is distributed in the northern area of China, and its main producing region is three provinces in the northeast of China, wherein, Liaoning,
Two province of Heilungkiang since the sixties in last century, by using Corylus heterophylla Fisch as male parent, introducing a fine variety the hybridization hazel research work that wood-nut is carried out
Make achieved with very big positive achievement.The big fruit Semen coryli heterophyllae almost all in flat Europe sold on existing China's nut market from the Liao Dynasty,
Black two province.The seed of Semen coryli heterophyllae is referred to as Semen coryli heterophyllae, wherein containing multiple proteins, vitamin and trace element etc., the wherein content of calcium
For 307 mg, it is 30 times of Fructus Mali pumilae;The content of ferrum be 7.85 mg, be Semen Armeniacae Amarum, 1 ~ 3 times of Semen Juglandis.Semen coryli heterophyllae is except rich in various
Outside the material beneficial to body metabolism, the unsaturated fatty acid for including and Vitamin E adjust blood pressure, reduce gallbladder to controlling blood fat
Sterin, prevents coronary heart disease, arteriosclerosis and diabetes from suffering from obvious action.In addition, the composition of Semen coryli heterophyllae is natural shape
Into, the material without pesticide, chemical fertilizer and any synthetic, it is most one of wholefood of nutritive value.Meanwhile, Corylus heterophylla Fisch
And as the preferable selection of the good parent that selection cross is carried out with other hazel kinds.More than the modes of reproduction of Semen coryli heterophyllae using press strip and
Seminal propagation, as mound layering belongs to vegetative manner, it is impossible to produce new Phenotype individual plants;The seed tool of Semen coryli heterophyllae
There is rear maturity, traditional seminal propagation then needs the mode through cool stratification just promote seed to sprout, required breeding week
Phase is longer, in order to improve status of the Corylus heterophylla Fisch in economic forest market, also for further reinforcement and other hazel kind selection cross
Success rate, shortening seed sprouting and growing-seedling period just becomes an important link.
Traditional Semen coryli heterophyllae seminal propagation entirety step is:1. to early September, annual August end treats that Corylus heterophylla Fisch fruit bud opens naturally,
The de- bud landing of nut is placed on ventilation storage after drying in the shade;2. select (before soil is freeze-up) at the beginning of 11 months physical features it is high and dry, without the plague of rats, in the shade
Where, dig zanjon or hole;3. take during husky and water mixes and seed is put into ditch or hole, puddle uniform together, then filled up with sand,
Straw screen or mat in upper cover;4. late April spring in next year sowing.Whole seed is sprouted will be after the time of 7 ~ 8 months.
The present invention compared with《A kind of breeding method of Corylus heterophylla Fisch seed》(Publication number 104054546A)Difference with advantage be:It is first
First, exist《A kind of breeding method of Corylus heterophylla Fisch seed》Main emphasis are the breeding method of Corylus heterophylla Fisch seed, and overall step is seed
Sand Tibetan → cool stratification → spring in the next year accelerating germination → sowing → final-period management of process → the previous year seed, formerly invention are adopted
It is the method for traditional husky Tibetan+cool stratification, needs through about 7-8 month from germination of collecting seed, i.e., 210-240 days left
The right time.The overall step of the present invention is seed-soaking → seed disinfection → HORMONE TREATMENT → sowing → final-period management, from adopting
Germination is planted, whole required times are about at 33-35 days or so(After 33 days, the Herb height of sprouting is up to 30cm
More than).Relatively formerly invention, with larger advantage, compares traditional Corylus heterophylla Fisch presprouting of seeds of more first invention to the present invention in time
Method, can save at least 70% time;It is different that innovative point employs disinfectant+phytohormone, peat soil+river sand etc.
Combination matching mode, the sprouting to Corylus heterophylla Fisch seed have carried out accelerating germination, and effect is significant.Test is proved, using the method for the present invention,
Efficiently can quickly make Corylus heterophylla Fisch seed be unearthed at short notice to sprout, and emergence rate is high, emerge neat, the main root fibrous root of plant
Healthy growth.
The content of the invention
Corylus heterophylla Fisch as subjects, by the concentration for the treatment of for finding suitable disinfectant and phytohormone, is looked for by the present invention
To can make Corylus heterophylla Fisch seed at short notice rudiment be unearthed new method.Compare traditional cool stratification method for culturing seedlings, the method
The cultivation time needed for Corylus heterophylla Fisch seed is sprouted can significantly be shortened(Only need to 27 days or so from collecting seed to sprouting, wherein waiting seed
Naturally departing from fruit bud needs about 15 days or so), and there is efficiency high of emerging(Sowing 30 days when emergence rate up to 35% with
On), growth of seedling it is quick(During growth of seedling 30 days or so, Herb length is up to 30 cm or so), simple operating steps etc. it is excellent
Point, it may be considered that promote as New technical use.
The present invention is achieved through the following technical solutions:
A kind of method of the efficient fast-germination of Corylus heterophylla Fisch seed, comprises the following steps:It is in mid or late September then, harvesting ripe, disease-free
The Corylus heterophylla Fisch seed of insect pest.The seed of the band fruit bud after harvesting is positioned at shady and cool drying, waits fruit bud to parch naturally, seed face
Complexion changed is brown after departing from naturally fruit bud, seed collection is got up to be placed on standby at shady and cool dry;
Water seed soaking process:The seed of the maturation got ready is put into into 35 DEG C of warm water, constant temperature seed soaking is carried out, process time 24 ~
48 h;
Seed disinfection is processed:Disinfectant is potassium permanganate(KMnO4)0.1 ~ 0.5% strength solution, soaking disinfection process, during process
Between be 1 ~ 3h;
Seed HORMONE TREATMENT:Hormone is phytohormone gibberellin(GA3)100 ~ 250ppm strength solutions, immersion treatment, during process
Between be 12 ~ 48 h;
Substrate is selected:Sowing media selects peat soil and thin river sand mix and match, mixed proportion 3:2;
Substrate is disinfected:Disinfectant is disinfected with seed with processing mode, and process time is 12 ~ 24 h;
Sowing container, ways and means:Sowing container selects seedling culture hole plate;Seeding method is using a broadcast mode;Type of seeding is,
Sterilization substrate is dried in the shade, and sterilization substrate is laid in nursery cave, thickness 2cm or so, one Corylus heterophylla Fisch seed of each nursery cave program request,
Hilum downwards, is covered with sterilization substrate above seed, is covered to tool nursery cave at 0.5 cm, is finally somewhat pressed substrate
It is real;
Management after planting and rush germination and growth environment:Seedling culture hole plate after planting supplements water supply in media in time, pours permeable, then
Hole tray is placed at room ventilation, ambient temperature is maintained at 23 ~ 25 DEG C, was hereafter sprayed water with watering can every 5 ~ 6 days, overall humidity
Maintain 70 ~ 75%;Receive natural scattering light irradiation daily, it is to avoid direct sunlight light irradiation substrate;
Sprouting situation:There is within 10th ~ 12 day seedling to sprout from the note that begins sowing day to be unearthed;When 21 days, seedling germination rate maintains 20%
Left and right;When 30 days, seedling germination rate is up to more than 35%;
Growing state:From after coming up about 10 days or so, seedling can grow 3 or so true leaves to Corylus heterophylla Fisch seed, and plant total length can
Up to 20 more than cm, root growth is normal, and main root physically well develops, and fibrous root is flourishing;
Beneficial effects of the present invention:The method combined with HORMONE TREATMENT by the sterilization adopted by the method, can make the Corylus heterophylla Fisch seed need not
Efficiently quickly can sprout through cool stratification.Experiment proves that, Corylus heterophylla Fisch seed germinateed at after planting 10 ~ 12 days, 30 days kinds
Sub- germination rate grows most fast seedling plant at 30 days or so up to more than 35%, and Herb overall length is up to 30 more than cm and main
Root development is good, and fibrous root is flourishing.Traditional cool stratification method for culturing seedlings is compared, the method can significantly shorten Corylus heterophylla Fisch seed and sprout institute
The cultivation time for needing, and the efficiency high that has the advantages that to emerge, emerge neat, growth of seedling quickly, simple operating steps.
Specific embodiment
Below in conjunction with the specific embodiment of the invention, technical scheme is clearly and completely described, is shown
So, described embodiment is only a part of embodiment of the invention, rather than the embodiment of whole.Based on the reality in the present invention
Example is applied, the every other embodiment obtained under the premise of creative work is not made by those of ordinary skill in the art all belongs to
In the scope of protection of the invention.
Embodiment 1
A kind of method of the efficient fast-germination of Corylus heterophylla Fisch seed, comprises the following steps:
The seed place gathered by this sprouting test is Mudanjiang City of Heilongjiang Province, in mid or late September then, harvesting ripe, nothing
The Corylus heterophylla Fisch seed of pest and disease damage(Together adopt down together with fruit bud).The seed of the band fruit bud after harvesting is positioned at shady and cool drying, etc.
Treat that fruit bud is parched naturally, after seed color is changed into brown and departs from naturally fruit bud, seed collection is got up to be placed on cool place it is dry at
It is standby;
Water seed soaking process:Ripe seed is first put in 35 DEG C of thermostat water baths carries out seed soaking, the depth of water in water-bath
10 cm, 24 ~ 48 h of process time;
Seed disinfection is processed:Then it is potassium permanganate with disinfectant(KMnO4)Solution, 0.2 ~ 0.4 % concentration, at soaking disinfection
Reason, process time are 1 ~ 2 h;
Seed HORMONE TREATMENT:It is phytohormone gibberellin with hormone again(GA3)Solution, 100 ~ 200 ppm concentration, immersion treatment,
Process time is 12 ~ 24 h;
Substrate is selected:Sowing media selects peat soil and thin river sand mix and match, mixed proportion 3:2;
Substrate is disinfected:Disinfectant is disinfected with seed with processing mode, and process time is 12 ~ 24 h.It is disposed, will
Substrate drains sterilization moisture;
Sowing container, ways and means:Sowing container selects seedling culture hole plate(Specification:4 × 4 × 5.5 cm, often disk nursery cave 3 × 4
It is individual);Seeding method is using a broadcast mode;Type of seeding is, sterilization substrate is dried in the shade to moisture content at 15 ~ 20% or so, with holding
Agglomerating, unclamping weak vibrations has phenomenon of scattering, and sterilization substrate is laid in nursery cave, thickness 2cm or so, each nursery cave point
A Corylus heterophylla Fisch seed is broadcast, hilum downwards, is covered with sterilization substrate above seed, covered to tool nursery cave at 0.5 cm, most
Afterwards substrate is somewhat compacted;
Promote germination and growth environment:Hole tray after planting is positioned over into interior, appropriate to divulge information, ambient temperature is maintained at 23 ~ 25 DEG C, whole
Body humidity maintains 70 ~ 75%(Water except period);
Management after planting:After planting water supply in media will be supplemented in time, pour permeable, hereafter sprayed water with watering can every 5 ~ 6 days, sprinkling
Standard has just had hydrops for surface matrix;Receive natural scattering light irradiation daily, it is to avoid direct sunlight light irradiation substrate,
If meeting overcast sky, fluorescent lamp supplementary light is opened;Hole tray putting position after planting will leave mutually certain ventilation space, to the greatest extent
Amount avoids hole tray two-by-two from being next to;
Sprouting situation:There is within 12nd day or so seedling to sprout from the note that begins sowing day to be unearthed;When 21 days, seedling germination rate is maintained
20% or so;When 30 days, seedling germination rate is up to more than 35%;
Growing state:From after coming up about 10 days or so, seedling can grow 3 or so true leaves to Corylus heterophylla Fisch seed, and plant total length can
Up to 20 more than cm;35 days or so, up to 30 more than cm, root growth was normal, and main root physically well develops, fibrous root for above-ground plant parts
It is flourishing;
The method combined with HORMONE TREATMENT by the sterilization adopted by the method, can make Corylus heterophylla Fisch seed need not move through height by cool stratification
Effect is quickly sprouted.Experiment proves that, Corylus heterophylla Fisch seed after planting 10 ~ 12 days germinate, 30 days seed germination rates up to 35% with
On, most fast seedling plant is grown at 30 days or so, Herb overall length physically well develops up to 30 more than cm, and main root, and fibrous root is sent out
Reach.Traditional cool stratification method for culturing seedlings is compared, the method can significantly shorten the cultivation time needed for Corylus heterophylla Fisch seed is sprouted, and
Having the advantages that to emerge, efficiency high, growth of seedling are quick, neat, simple operating steps of emerging.
Comparative example 1(Seed is without warm water soaking process)
In mid or late September then, harvesting ripe, the Corylus heterophylla Fisch seed of no disease and pests harm(Together adopt down together with fruit bud).After harvesting
Seed with fruit bud is positioned at shady and cool drying, waits fruit bud to parch naturally, and seed color is changed into brown and departs from naturally fruit bud
Afterwards, seed collection is got up to be placed on standby at shady and cool drying;
Seed disinfection is processed:Carry out disinfection after wash seeds, disinfectant is potassium permanganate(KMnO4)Solution, 0.1 ~ 0.3 % are dense
Degree, soaking disinfection process, process time are 1 ~ 2 h;
Seed HORMONE TREATMENT:Hormone is phytohormone gibberellin(GA3)Solution, 150 ~ 250 ppm concentration, immersion treatment are processed
Time is 12 ~ 24 h;
Substrate is selected:Sowing media selects peat soil, mixed proportion 3:2;
Substrate is disinfected:Disinfectant is disinfected with seed with processing mode, and process time is 12 ~ 24 h.It is disposed, will
Substrate drains sterilization moisture;
Sowing container, ways and means:Sowing container selects seedling culture hole plate(Specification:4 × 4 × 5.5 cm, often disk nursery cave 3 × 4
It is individual);Seeding method is using a broadcast mode;Type of seeding is, sterilization substrate is dried in the shade to moisture content at 15 ~ 20% or so(With holding
Agglomerating, unclamping weak vibrations has phenomenon of scattering), sterilization substrate is laid in nursery cave, thickness 2cm or so, each nursery cave
One Corylus heterophylla Fisch seed of program request, hilum downwards, are covered with sterilization substrate above seed, are covered to tool nursery cave at 0.5 cm,
Finally substrate is somewhat compacted;
Promote germination and growth environment:Hole tray after planting is positioned over into interior, appropriate to divulge information, ambient temperature is maintained at 23 ~ 25 DEG C, whole
Body humidity maintains 70 ~ 75%(Water except period);
Management after planting:After planting water supply in media will be supplemented in time, pour permeable, hereafter sprayed water with watering can every 5 ~ 6 days, sprinkling
Standard has just had hydrops for surface matrix;Receive natural scattering light irradiation daily, it is to avoid direct sunlight light irradiation substrate,
If meeting overcast sky, fluorescent lamp supplementary light is opened;Hole tray putting position after planting will leave mutually certain ventilation space, to the greatest extent
Amount avoids hole tray two-by-two from being next to;
Sprouting situation:From the note the 33rd day of beginning sowing day, there are 8 seed seedling to sprout and be unearthed, germination rate is 7.4%;
Growing state:From after coming up about 15 days or so, seedling can grow 3 or so true leaves to Corylus heterophylla Fisch seed, and plant total length can
Up to 20 cm or so, root growth is normal, and main root physically well develops, and fibrous root is flourishing;
Comparative example 2(The non-sterile process of seed)
In mid or late September then, harvesting ripe, the Corylus heterophylla Fisch seed of no disease and pests harm(Together adopt down together with fruit bud).After harvesting
Seed with fruit bud is positioned at shady and cool drying, waits fruit bud to parch naturally, and seed color is changed into brown and departs from naturally fruit bud
Afterwards, seed collection is got up to be placed on standby at shady and cool drying;
Water seed soaking process:Ripe seed is put in 35 DEG C of thermostat water baths carries out seed soaking, the depth of water 10 in water-bath
Cm, 36 ~ 48 h of process time;
Seed HORMONE TREATMENT:Hormone is phytohormone gibberellin(GA3)Solution, 150 ~ 250 ppm concentration, immersion treatment are processed
Time is 36 ~ 48 h;
Substrate is selected:Sowing media selects peat soil, mixed proportion 3:2;
Substrate is disinfected:Disinfectant is disinfected with seed with processing mode, and process time is 0 ~ 12 h.It is disposed, will
Substrate drains sterilization moisture;
Sowing container, ways and means:Sowing container selects seedling culture hole plate(Specification:4 × 4 × 5.5 cm, often disk nursery cave 3 × 4
It is individual);Seeding method is using a broadcast mode;Type of seeding is, sterilization substrate is dried in the shade to moisture content at 15 ~ 20% or so, with holding
Agglomerating, unclamping weak vibrations has phenomenon of scattering, and sterilization substrate is laid in nursery cave, thickness 2cm or so, each nursery cave point
A Corylus heterophylla Fisch seed is broadcast, hilum downwards, is covered with sterilization substrate above seed, covered to tool nursery cave at 0.5 cm, most
Afterwards substrate is somewhat compacted;
Promote germination and growth environment:Hole tray after planting is positioned over into interior, appropriate to divulge information, ambient temperature is maintained at 23 ~ 25 DEG C, whole
Body humidity maintains 70 ~ 75%(Water except period);
Management after planting:After planting water supply in media will be supplemented in time, pour permeable, hereafter sprayed water with watering can every 5 ~ 6 days, sprinkling
Standard has just had hydrops for surface matrix;Receive natural scattering light irradiation daily, it is to avoid direct sunlight light irradiation substrate,
If meeting overcast sky, fluorescent lamp supplementary light is opened;Hole tray putting position after planting will leave mutually certain ventilation space, to the greatest extent
Amount avoids hole tray two-by-two from being next to;
Sprouting situation:From the note the 33rd day of beginning sowing day, there are 12 seed seedling to sprout and be unearthed, germination rate is 11.1%;Dig out other
Seed is shown in mildew and rot phenomenon;
Growing state:Unearthed growth of seedling is normal.
Comparative example 3(Seed is without HORMONE TREATMENT)
In mid or late September then, harvesting ripe, the Corylus heterophylla Fisch seed of no disease and pests harm(Together adopt down together with fruit bud).After harvesting
Seed with fruit bud is positioned at shady and cool drying, waits fruit bud to parch naturally, and seed color is changed into brown and departs from naturally fruit bud
Afterwards, seed collection is got up to be placed on standby at shady and cool drying;
Water seed soaking process:Ripe seed is put in 35 DEG C of thermostat water baths carries out seed soaking, the depth of water 10 in water-bath
Cm, 36 ~ 48 h of process time;
Seed disinfection is processed:Disinfectant is potassium permanganate(KMnO4)Solution, 0.4 ~ 0.5 % concentration, soaking disinfection process are processed
Time is 1 ~ 3 h;
Substrate is selected:Sowing media selects peat soil and thin river sand mix and match, mixed proportion 3:2;
Substrate is disinfected:Disinfectant is disinfected with seed with processing mode, and process time is 12 ~ 24 h.It is disposed, will
Substrate drains sterilization moisture;
Sowing container, ways and means:Sowing container selects seedling culture hole plate(Specification:4 × 4 × 5.5 cm, often disk nursery cave 3 × 4
It is individual);Seeding method is using a broadcast mode;Type of seeding is, sterilization substrate is dried in the shade to moisture content at 15 ~ 20% or so, with holding
Agglomerating, unclamping weak vibrations has phenomenon of scattering, and sterilization substrate is laid in nursery cave, thickness 2cm or so, each nursery cave point
A Corylus heterophylla Fisch seed is broadcast, hilum downwards, is covered with sterilization substrate above seed, covered to tool nursery cave at 0.5 cm, most
Afterwards substrate is somewhat compacted;
Promote germination and growth environment:Hole tray after planting is positioned over into interior, appropriate to divulge information, ambient temperature is maintained at 23 ~ 25 DEG C, whole
Body humidity maintains 70 ~ 75%(Water except period);
Management after planting:After planting water supply in media will be supplemented in time, pour permeable, hereafter sprayed water with watering can every 5 ~ 6 days, sprinkling
Standard has just had hydrops for surface matrix;Receive natural scattering light irradiation daily, it is to avoid direct sunlight light irradiation substrate,
If meeting overcast sky, fluorescent lamp supplementary light is opened;Hole tray putting position after planting will leave mutually certain ventilation space, to the greatest extent
Amount avoids hole tray two-by-two from being next to;
Sprouting situation:From the note the 33rd day or so that begins sowing day, there are 2 seed seedling to sprout and be unearthed, germination rate is 1.8%;
Growing state:Unearthed growth of seedling is normal, but the speed of growth is slower.
Comparative example 4(Conventional cryogenic stratification method)
In mid or late September then, harvesting ripe, the Corylus heterophylla Fisch seed of no disease and pests harm(Together adopt down together with fruit bud).After harvesting
Seed with fruit bud is positioned at shady and cool drying, waits fruit bud to parch naturally, and seed color is changed into brown and departs from naturally fruit bud
Afterwards, seed collection is got up to be placed on standby at shady and cool drying;
Water seed soaking process:Ripe seed is put in 35 DEG C of thermostat water baths carries out seed soaking, the depth of water 10 in water-bath
Cm, 12 ~ 24 h of process time;
Seed disinfection is processed:Disinfectant is potassium permanganate(KMnO4)Solution, 0.3 ~ 0.4 % concentration, soaking disinfection process are processed
Time is 2 ~ 3 h;
Substrate is selected:Sowing media selects peat soil and thin river sand mix and match, mixed proportion 3:2;
Substrate is disinfected:Disinfectant is disinfected with seed with processing mode, and process time is 12 ~ 24 h.It is disposed, will
Substrate drains sterilization moisture;
Accelerating germination container, ways and means:Sowing container selects carton box(Specification:30×30×30 cm);Accelerating germination mode adopts layer
Product mode;Germination accelerating method is, sterilization substrate is dried in the shade to moisture content at 15 ~ 20% or so, agglomerating with holding, and unclamps weak vibrations
There is phenomenon of scattering, sterilization substrate is laid in into one layer of carton box bottom, 5 cm of thickness or so;Be used above a broadcast mode be put into it is flat
Hazel seed, piles one layer;One layer of sterilization substrate, about 3 cm of thickness or so are repaved above seed;Last layer seed is repaved in substrate,
So operate at 5 cm of box top and spread last layer of substrate;
Stratification growing environment:Carton box after lamination is positioned over into fridge freshness retaining layer(4℃)In environment, sprayed every 8 ~ 10 days
Once, overall humidity maintains 50 ~ 55% to water(Water except period);
Sprouting situation:From the note the 33rd day or so that begins sowing day, sprouting phenomenon is there are no(This embodiment is used as controlled trial group).
From the point of view of passing through the test of done various embodiments, in addition to embodiment 1, other 4 embodiments all exist various
Different the drawbacks of, now by 4 embodiments the drawbacks of illustrate and analysis:In embodiment 2, germination percentage 7.4% or so, seed
Though there is sprouting, germination rate is low, and sprout time is longer.Reason is in this embodiment, the step of lacked water seed soaking.
As Semen coryli heterophyllae seed belongs to nutses, with more hard kind shell, record according to documents and materials, the measure of some stratifications
In, the kind shell of seed can be struck and be split to help embryo germination;But the embryo of seed is easily damaged by this kind of way, does not advocate use.Adopt
With water seed soaking process, the kind shell osmotic absorbent of drying can be made, is contributed to kind of a shell and is voluntarily ftractureed, embryo germination;In embodiment 3,
Lack sterilisation step, cause seed to go rotten, it can be seen that, the sterilization and the sterilisation step of sprouting substrate that Semen coryli heterophyllae seed is sprouted is
Important;And, to sprout substrate and only use peat soil a kind of, poor air permeability is also one of the reason for causing seed to go rotten, so
In follow-up embodiment, the substrate combination that substrate has selected peat soil and thin river sand mix and match is sprouted, to ensure substrate
Permeable breathable, test proving effect are good;In embodiment 4, the step of lacked HORMONE TREATMENT, by after planting the 33rd day,
There are no seed and sprout phenomenon, it is possible thereby to illustrate, the seed of Semen coryli heterophyllae has certain dormancy and rear maturity, with finite concentration
Phytohormone the physiological activity of Interior Seed is aggravated environmental stimuli is carried out to Semen coryli heterophyllae seed, accelerate seed and sprout
Speed is sent out, so as to the germination rate for efficiently quickly improving Semen coryli heterophyllae seed;5 used indoors artificial of embodiment imitates traditional hazel
The method of the outer stratification of sub- seed chamber, this embodiment are treating as controlled trial group.Equally by the after planting the 33rd
My god, there are no seed and sprout phenomenon.Consider it, the sprouting of the method for embodiment 1 and measure to Corylus heterophylla Fisch seed has efficient
Quick facilitation, and it is easy to operate, it is easy to promote.
Claims (1)
1. the method for the efficient fast-germination of a kind of Corylus heterophylla Fisch seed, it is characterised in that comprise the following steps:
(1)In mid or late September then, harvesting ripe, the Corylus heterophylla Fisch seed of no disease and pests harm, the seed of the band fruit bud after harvesting is placed
Place is dried in shady and cool, waits fruit bud to parch naturally, seed color is changed into brown and after disengaging is really luxuriant naturally, and seed collection is got up
It is placed on standby at shady and cool drying;
(2)Water seed soaking process:The seed got ready is put in 35 DEG C of warm water, constant temperature seed soaking, process time 24 ~ 48 is carried out
h;
(3)Seed disinfection is processed:Disinfectant is potassium permanganate(KMnO4)0.1 ~ 0.5% strength solution, soaking disinfection process, is processed
Time is 1 ~ 3 h;
(4)Seed HORMONE TREATMENT:Hormone is phytohormone gibberellin(GA3)100 ~ 250 ppm strength solutions, immersion treatment are processed
Time is 12 ~ 48 h;
(5)Substrate is selected:Sowing media selects peat soil and thin river sand mix and match, mixed proportion 3:2;
(6)Substrate is disinfected:Disinfectant is potassium permanganate(KMnO4)0.1 ~ 0.5% strength solution, soaking disinfection process, is processed
Time is 12 ~ 24 h;
(7)Sowing container, ways and means:Sowing container selects seedling culture hole plate;Seeding method is using a broadcast mode;Type of seeding
For the sterilization substrate after process is dried in the shade, and the sterilization substrate after drying in the shade is laid in nursery cave, thickness 2cm, each nursery cave point
Broadcast a Corylus heterophylla Fisch seed Jing after above three step process, hilum downwards, is covered with sterilization substrate above seed, cover to away from
On nursery cave at 0.5 cm, finally substrate is somewhat compacted;
(8)Management after planting and rush germination and growth environment:Seedling culture hole plate after planting supplements water supply in media in time, pours permeable,
Then hole tray is placed at room ventilation, ambient temperature is maintained at 23 ~ 25 DEG C, was hereafter sprayed water with watering can every 5 ~ 6 days, it is overall
Humidity maintains 70 ~ 75%;Receive natural scattering light irradiation daily, it is to avoid direct sunlight light irradiation substrate;
(9)Sprouting situation:Starting note day from sowing has seedling sprouting to be unearthed on the 10th ~ 12 day;When 21 days, seedling germination rate dimension
Hold more than 20%;When 30 days, seedling germination rate is up to more than 35%;
(10)Growing state:From after coming up 10 days, seedling can grow 3 or so true leaves to Corylus heterophylla Fisch seed, and plant total length is reachable
20 more than cm, root growth are normal, and main root physically well develops, and fibrous root is flourishing.
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Cited By (6)
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CN107295830A (en) * | 2017-08-18 | 2017-10-27 | 吉林省林业科学研究院 | A kind of germination accelerating method of flat hazel seed |
CN108834473A (en) * | 2018-07-18 | 2018-11-20 | 南京林业大学 | A method of releasing seed dormancy |
CN109565998A (en) * | 2018-12-24 | 2019-04-05 | 广西壮族自治区林业科学研究院 | A method of promote Antigonon leptopus seed to sprout |
CN113068475A (en) * | 2021-03-05 | 2021-07-06 | 辽宁省经济林研究所 | Seedling raising method for shortening hazelnut cross breeding period |
CN113892319A (en) * | 2021-10-30 | 2022-01-07 | 烟台市林业科学研究所 | Method for relieving hazelnut seed dormancy and promoting germination by using thiourea |
WO2023083284A1 (en) * | 2021-11-11 | 2023-05-19 | 贵州省生物研究所 | Rapid cultivation method for semi-aseptic hazelnut seedlings |
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CN107295830A (en) * | 2017-08-18 | 2017-10-27 | 吉林省林业科学研究院 | A kind of germination accelerating method of flat hazel seed |
CN107295830B (en) * | 2017-08-18 | 2020-12-08 | 吉林省林业科学研究院 | Germination accelerating method for corylus heterophylla bunge seeds |
CN108834473A (en) * | 2018-07-18 | 2018-11-20 | 南京林业大学 | A method of releasing seed dormancy |
CN109565998A (en) * | 2018-12-24 | 2019-04-05 | 广西壮族自治区林业科学研究院 | A method of promote Antigonon leptopus seed to sprout |
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CN113068475A (en) * | 2021-03-05 | 2021-07-06 | 辽宁省经济林研究所 | Seedling raising method for shortening hazelnut cross breeding period |
CN113892319A (en) * | 2021-10-30 | 2022-01-07 | 烟台市林业科学研究所 | Method for relieving hazelnut seed dormancy and promoting germination by using thiourea |
CN113892319B (en) * | 2021-10-30 | 2023-08-22 | 烟台市森林资源监测保护服务中心(烟台沿海防护林省级自然保护区管理服务中心、烟台市林业科学研究所) | Method for relieving dormancy of hazelnut seeds and promoting germination by using thiourea |
WO2023083284A1 (en) * | 2021-11-11 | 2023-05-19 | 贵州省生物研究所 | Rapid cultivation method for semi-aseptic hazelnut seedlings |
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