CN106417117A - Yalong River schizothorax wangchiachii artificial proliferation and release method - Google Patents
Yalong River schizothorax wangchiachii artificial proliferation and release method Download PDFInfo
- Publication number
- CN106417117A CN106417117A CN201610853527.8A CN201610853527A CN106417117A CN 106417117 A CN106417117 A CN 106417117A CN 201610853527 A CN201610853527 A CN 201610853527A CN 106417117 A CN106417117 A CN 106417117A
- Authority
- CN
- China
- Prior art keywords
- water
- days
- fry
- artificial
- short hairs
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 40
- 241000066295 Schizothorax wangchiachii Species 0.000 title abstract 5
- 230000035755 proliferation Effects 0.000 title abstract 3
- 241000251468 Actinopterygii Species 0.000 claims abstract description 41
- 230000002196 ecbolic effect Effects 0.000 claims abstract description 21
- 230000012447 hatching Effects 0.000 claims abstract description 20
- 238000009395 breeding Methods 0.000 claims abstract description 9
- 230000001488 breeding effect Effects 0.000 claims abstract description 9
- 238000002347 injection Methods 0.000 claims abstract description 5
- 239000007924 injection Substances 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 63
- 210000004209 hair Anatomy 0.000 claims description 36
- 239000003814 drug Substances 0.000 claims description 20
- 229940079593 drug Drugs 0.000 claims description 20
- 210000004602 germ cell Anatomy 0.000 claims description 13
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 11
- 150000001875 compounds Chemical class 0.000 claims description 11
- 229910052760 oxygen Inorganic materials 0.000 claims description 11
- 239000001301 oxygen Substances 0.000 claims description 11
- 239000012528 membrane Substances 0.000 claims description 10
- 102000002322 Egg Proteins Human genes 0.000 claims description 8
- 108010000912 Egg Proteins Proteins 0.000 claims description 8
- 210000004681 ovum Anatomy 0.000 claims description 8
- 230000008569 process Effects 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 7
- 230000004083 survival effect Effects 0.000 claims description 7
- 206010002091 Anaesthesia Diseases 0.000 claims description 6
- 241001290771 Limnodrilus hoffmeisteri Species 0.000 claims description 6
- 230000037005 anaesthesia Effects 0.000 claims description 6
- 210000000582 semen Anatomy 0.000 claims description 5
- 241001247197 Cephalocarida Species 0.000 claims description 4
- 241000252233 Cyprinus carpio Species 0.000 claims description 4
- 206010062767 Hypophysitis Diseases 0.000 claims description 4
- 230000037396 body weight Effects 0.000 claims description 4
- 230000000694 effects Effects 0.000 claims description 4
- 210000003746 feather Anatomy 0.000 claims description 4
- 230000026109 gonad development Effects 0.000 claims description 4
- 239000005556 hormone Substances 0.000 claims description 4
- 229940088597 hormone Drugs 0.000 claims description 4
- 210000000006 pectoral fin Anatomy 0.000 claims description 4
- 230000004044 response Effects 0.000 claims description 4
- 230000034994 death Effects 0.000 claims description 3
- 231100000517 death Toxicity 0.000 claims description 3
- 230000004720 fertilization Effects 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 238000012545 processing Methods 0.000 claims description 3
- 102000011022 Chorionic Gonadotropin Human genes 0.000 claims description 2
- 108010062540 Chorionic Gonadotropin Proteins 0.000 claims description 2
- 229940015047 chorionic gonadotropin Drugs 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 102000009151 Luteinizing Hormone Human genes 0.000 claims 1
- 108010073521 Luteinizing Hormone Proteins 0.000 claims 1
- 239000000835 fiber Substances 0.000 claims 1
- 239000003668 hormone analog Substances 0.000 claims 1
- 230000032696 parturition Effects 0.000 claims 1
- 229920006395 saturated elastomer Polymers 0.000 claims 1
- 230000008901 benefit Effects 0.000 abstract description 7
- 230000007613 environmental effect Effects 0.000 abstract description 5
- 238000011084 recovery Methods 0.000 abstract description 3
- 230000009027 insemination Effects 0.000 abstract 1
- 235000019688 fish Nutrition 0.000 description 36
- 230000003187 abdominal effect Effects 0.000 description 5
- 230000001568 sexual effect Effects 0.000 description 4
- 206010002660 Anoxia Diseases 0.000 description 3
- 241000976983 Anoxia Species 0.000 description 3
- 206010021143 Hypoxia Diseases 0.000 description 3
- 230000007953 anoxia Effects 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 239000003488 releasing hormone Substances 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 210000001015 abdomen Anatomy 0.000 description 2
- 230000000386 athletic effect Effects 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 210000002969 egg yolk Anatomy 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 210000004907 gland Anatomy 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 210000001672 ovary Anatomy 0.000 description 2
- 238000006213 oxygenation reaction Methods 0.000 description 2
- 238000012856 packing Methods 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 238000012549 training Methods 0.000 description 2
- 238000010200 validation analysis Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 210000004247 hand Anatomy 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 238000003911 water pollution Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a Yalong River schizothorax wangchiachii artificial proliferation and release method. The method includes the seven steps of parent fish selection, oxytocic injection, artificial insemination, zygote hatching, fish larvae breeding, advanced fry breeding and transport and release. Yalong River schizothorax wangchiachii has extremely high economic value. Artificial proliferation and release of Yalong River schizothorax wangchiachii are achieved, and influences of hydro-junctions on schizothorax wangchiachii populations can be effectively reduced. Besides, wild resource recovery is facilitated, basic resources are provided for biological study, and economic and environmental ecological benefits are great.
Description
Technical field
Present invention relates particularly to a kind of artificial fecundation of Yalongjiang River short hairs schizothoracin releases method.
Background technology
Yalongjiang River is the maximum tributary of Upper Yangtze River Jinsha jiang River left bank, and it is important that short hairs schizothoracin is not only Yalong river valley
Economic fish resource, is Upper Yangtze River Endemic fish simultaneously, is distributed mainly on the water such as Dadu River, Yalongjiang River, Jinsha jiang River and the Wujiang River
System, has important function in terms of maintaining Yalongjiang River aquatic ecosystem balance.In recent years, fished for due to transition, water pollution,
Hydraulic engineering and adopt the anthropic factors such as sand so that natural short hairs schizothoracin stock number degradation, have impact on society, warp in basin
Ji and the benign cycle of ecological environment.
At present, many researcheres are also had to adopt the method for artificial fecundation to cultivate short hairs schizothoracin, the method is typically to utilize
The wild sexual maturity adult fish fishing acquisition arranges more original incubator and carries out as parent fish, then on the Yalongjiang River nature water surface
The artificial incubation of fish roe, is hatched using environmental conditions such as natural temperature, current；Although the method splits abdomen for increasing short hairs
Fish fry quantity has certain help, but yet suffers from a lot of shortcomings and deficiencies:
（1）Short hairs schizothoracin poor growth, sexual maturity is slow, and rate of fertilization is low, is unfavorable for schizothoracin amount reproduction；
（2）Environment of hatching is to carry out under field conditions (factors), is susceptible to change, leads to rate of fertilization survival rate, emergence rate and survive
Rate is relatively low, is extremely unfavorable for the cultivation of schizothoracin.
Content of the invention
The technical problem to be solved in the present invention is：It is directed to deficiency of the prior art, provide a kind of Yalongjiang River short hairs to split
The artificial fecundation of abdomen fish releases method, and the method is effectively recovered and protected the short hairs schizothoracin resource of Yalongjiang River, for maintaining
The ecosystem balance in Yalongjiang River waters has made significant contribution, also allows the high short hairs schizothoracin of economic worth play more simultaneously
Good economic benefit, and this technique is solid, workable, large-scale production can be carried out.
In order to reach foregoing invention purpose, the technical solution used in the present invention is：A kind of Yalongjiang River short hairs schizothoracin is provided
Enhancement releasing method, including：
（One）Select physically fit, sexually matured Yalongjiang River short hairs schizothoracin raun and milter as parent fish, using anesthetis MS-
222 60 ~ 120/L anaesthetizes to the parent fish selecting, and the pectoral fin root then at parent fish injects oxytocic drug, at 13～16 DEG C
Lower effect 36 ~ 48 hours；Wherein, the oxytocic drug used by every kg body weight raun is grouped into by the group of following weight：Medulla Cyprinus carpio
Hypophysis 1mg+ chorionic-gonadotropin hormone 16ug+ luteotropin releasing hormone d-ala analog 1400IU；The consumption of milter oxytocic drug
Half for raun；
（Two）Gonad development degree inspection is carried out to the parent fish injecting oxytocic drug, adopts dry method people to developing preferable raun
Work is inseminated, and its gonopore and neighbouring body surface dry towel is dried moisture, gently extrudes abdominal part with handss and ovum is extruded into dry appearance
In device, then with same method, milter seminal fluid is clamp-oned in above-mentioned drying receptacle and lightly make seminal fluid and ovum mixing with feather stirring
Uniformly, it is subsequently adding clear water and continues stirring 10s, stand 2 minutes, be eventually adding a large amount of clear water stirring and washing unstickings, finally will be subject to
The water drenching hatching in hatching frame of smart ovum；
（Three）Hatch above-mentioned germ cell, Spawning water temperature is 13-17 DEG C, and indoor hatching adopts fluorescent lamp lighting；
（Four）Germ cell membrane after hatching, enters the larvae cultivation stage；8-10 days after prelarva membrane, entered with artemia nauplii
Row opening feeds；After 13-15 days, by the Limnodrilus hoffmeisteri mincing and artifical compound feed according to 1:Carry out after 1 weight proportion mixing
Raise；After 25-28 days, fed using artifical compound feed completely；
（Five）After fry grows to 2-3 centimetre, control raising the fry density in cylinder in 1-1.1 ten thousand tails/m2, every morning,
Noon and evening respectively feed once, and the standard that feeds is showed a small surplus by feedstuff and is defined, and often continuous feeding, after 7 days, stops feeding one day；
（Six）Treat that fry total length reaches 3-5 centimetre, proceed to postlarva breeding phase；Fry is proceeded in big specification cultivation fish pond and carries out
Flowing water is raised, and arranges fountain type water inlet in fishpond, and water inlet is located at 4-6m directly over fishpond, directly splashes in waterfall shape
Enter pond, water depth 70-100cm, water pH value is 7.0-8.0；Fry density is 500,000 tails/mu；Entered using artifical compound feed
Row is fed, and feeding number of times is that the standard that feeds is showed a small surplus by feedstuff and is defined sooner or later twice daily；
（Seven）After postlarva grow to total length be more than 7 centimetres after, carry out artificial releasing；When releasing, using anesthetis MS-222 20 ~ 40
It is calm that/L carries out anesthesia to fry, it is to avoid postlarva mutually extrudes in response to swashing and collides etc. and causes to damage and dead, and raising is put
Survival rate after stream.
Step（One）In, the consumption of anesthetis MS-222 is 6mg/L；After injection oxytocic drug, at 15 DEG C, effect 36 is little
When.
Step（Three）In, the actual conditions of incubating oosperm is：Will be through step（Two）The germ cell processing water temperature 13 ~
17 DEG C, the depth of water be 50cm, dissolved oxygen be more than 7mg/L, flow velocity be 2.0 ~ 2.5m/s hatchery in hatching 3 ~ 4 days, then by
Flow velocity is down to 1.0-1.5m/s hatching 3-5 days.
Step（Three）In, the actual conditions of incubating oosperm is：Will be through step（Two）The germ cell processing is in water temperature 15
DEG C, the depth of water be 50cm, dissolved oxygen be 7.5mg/L, flow velocity be 2.0m/s hatchery in hatching 4 days, then flow velocity is down to
1.0m/s is hatched 3 days.
The invention has the advantages that：
The Yalongjiang River short hairs schizothoracin artificial fecundation of the present invention releases method and is injected, manually awarded using parent fish selection, oxytocic drug
Essence, incubating oosperm, larvae cultivation, postlarva are cultivated and transport releases seven steps, the above-mentioned step with specific process conditions
It is combined with each other it is achieved that the enhancement releasing of Yalongjiang River short hairs schizothoracin, be effectively improved the resource of Yalongjiang River short hairs schizothoracin
Amount, not only protects Yalongjiang River short hairs schizothoracin population, and the high Yalongjiang River short hairs schizothoracin of value can be made to play more preferably
Economic benefit, there is huge using value.
By preparing the oxytocic drug with specific components and content, while not injuring short hairs schizothoracin, also effectively
Promote the gonad development of male and female parent fish completely, make the heat simultaneously of most of parent fishs simultaneously, improve the efficiency of artificial induced spawning, more favorably
In advanced fry cultivation and management work.
By using specific method for incubating oosperm, substantially increasing incubation rate and the survival rate of short hairs schizothoracin, being
The large scale cultivating of short hairs schizothoracin is had laid a good foundation.
Cultivation fish pond is arranged to staged by the present invention, arranges fountain type water inlet, cultivation fish pond profit in fishpond simultaneously
With itself drop, water is directly fallen in pond as waterfall, not only avoid big flow to enter water and impact is produced to fry, reduce
Fry physical ability is lost, but also greatly increases water dissolved oxygen amount, it is to avoid in breeding process, anoxia phenomenon occurs, and effectively increases fish
The survival rate of Seedling cultivation.
The inventive method achieves the enhancement releasing of Yalongjiang River short hairs schizothoracin, can not only effectively reduce key water control project
Impact to short hairs schizothoracin population, and contribute to the recovery of its wild resource amount, provide for its biological study simultaneously
Basic data, has great economy and environmental ecological benefit.
Brief description
Fig. 1 is the structural representation of cultivation fish pond.
1st, fountain type water inlet；2nd, fishpond.
Specific embodiment
With reference to specific embodiment, the specific embodiment of the present invention is described in detail：
Embodiment 1
The present embodiment with the short hairs schizothoracin enhancement releasing technique in Yalongjiang River silk screen-Guandi Hydropower Station fish multiplication pouring station is
Example, this pouring station is made great efforts through for many years, releases technique to short hairs schizothoracin artificial fecundation and is studied, has captured this kind
Artificial fecundation release in multiple key technologies, short hairs schizothoracin artificial fecundation release technique will for Yalongjiang River fishery resources and
More positive effect is played in the recovery of ecological environment and protection；It is specially：
Choose in Yalongjiang River capture physically fit, sexually matured short hairs schizothoracin raun and milter as parent fish, using anesthesia
Agent MS-222 6mg/L anaesthetizes to the parent fish selecting, and the pectoral fin base portion then at parent fish injects oxytocic drug, imitates at 15 DEG C
Answer 36 hours；Every oxytocic drug used by kg body weight raun is grouped into by the group of following weight：Cyprinus carpio hypophysis cerebri 1mg+ fine hair
Film promoting sexual gland hormone 16 μ g+ luteotropin releasing hormone d-ala analog 1400IU；Milter oxytocic drug consumption is the half of raun；Should
Oxytocic drug helps lend some impetus to male and female parent fish gonad development completely, and makes the heat simultaneously of most of parent fishs, improves artificial induced spawning
Efficiency, be more beneficial for advanced fry cultivation and management work.
Fully-developed raun after screening abdominal part expands obvious, the athletic injection through oxytocic drug carries out dry method people
Work is inseminated, and its gonopore and neighbouring body surface dry towel is dried moisture, is gently squeezed from top to bottom on raun abdominal part top with finger
Ovary, till ovum grain can not flow out naturally, is clamp-oned milter seminal fluid using same method at once after taking ovum and is softly stirred with feather
Mix, continue stir about 10s after being subsequently adding a small amount of clear water, stand 2 minutes, be eventually adding a large amount of clear water stirring and washing unsticking,
And water drenching in hatching frame is hatched by germ cell；
Incubating oosperm is the process that fertilized fish roe makes the smooth membrane of fry under certain artificial condition, during hatching, using day
Light lamp illuminate, and 15 DEG C of water temperature, the depth of water be 50cm, dissolved oxygen be 7.5mg/L, flow velocity be 2.0m/s hatchery in hatch 4
My god, then flow velocity is down to 1.0m/s and hatches 3 days；
Germ cell membrane after hatching, enters the larvae cultivation stage；In larvae cultivation, after fry membrane, 8-10 days itself yolk is inhaled
Harvest complete, start to carry out opening feeding with artemia nauplii；After 13 days, with the Limnodrilus hoffmeisteri that minces and artifical compound feed according to
Weight proportion 1:1 mixed material is raised, and is wherein gradually lowered the consumption of Limnodrilus hoffmeisteri with the growth of small fish, after 25 days,
Fed using artifical compound feed completely.
After fry grows to 2-3 centimetre, control raising the fry density in cylinder in 10,000 tails/m2, every morning, noon
Respectively feed once with evening, the standard that feeds is showed a small surplus by feedstuff and is defined, often continuous feeding, after 7 days, stops feeding one day.
Fry total length reaches 3 ~ 5cm and proceeds to postlarva breeding phase；Now fry is proceeded in big specification cultivation fish pond and carry out
Flowing water is raised, the high 130cm in fishpond pond, depth of water 100cm, and fishpond flowing water pH value is 7.0；As shown in figure 1, setting spray in fishpond 2
Spring formula water inlet 1, fountain type water inlet is located at the normal position 4m of fishpond, directly splashes into pond in waterfall shape；This fishpond 2 is in
Staged is distributed, and for usual water inlet is directly accessed culturing pool for duct type, this fishpond is by high-rise tuber drainage
To lower floor pond, the mouth of pipe is in flat, therefore, makes water directly fall pond as waterfall using this itself drop of cultivation platform
In, big flow on the one hand can be avoided to enter the injury that water impacts to fry, reduce the loss of fry physical ability；On the other hand can increase
Water dissolved oxygen amount, it is to avoid in breeding process, anoxia phenomenon occurs；In this fishpond, fry density is 500,000 tails/mu, and bait adopts
Artifical compound feed, feeding number of times is that daily sooner or later respectively once being satiated with food to feed is showed a small surplus as standard with feedstuff, until fry life
Enhancement releasing can be used for after packing when growing to 7 centimetres of total length.
Transport releases the fry being possessed the condition of releasing and carries out through fountain type water inlet, after oxygen supplement net training, being caught with net
It is contained in the fish bag of oxygenation after dragging for, then releases to corresponding location, using anesthetis MS-222 2mg/L, fry is carried out when releasing
Anesthesia is calm, it is to avoid postlarva mutually extrudes in response to swashing and collision causes body injures and deaths, and then raising releases survival rate.
Using the method for the present embodiment, about 2,000,000 tail short hairs schizothoracin original seed fries, warp are released to Yalongjiang River successively
Repeatedly validation trial, technique is solid, is at home and abroad pioneering, has significant economic, society and environmental ecology
Benefit.
Embodiment 2
Choose in Yalongjiang River capture physically fit, sexually matured short hairs schizothoracin raun and milter as parent fish, using anesthesia
Agent MS-222 12mg/L anaesthetizes to the parent fish selecting, and the pectoral fin base portion then at parent fish injects oxytocic drug, imitates at 16 DEG C
Answer 48 hours；Every oxytocic drug used by kg body weight raun is grouped into by the group of following weight：Cyprinus carpio hypophysis cerebri 1mg+ fine hair
Film promoting sexual gland hormone 16 μ g+ luteotropin releasing hormone d-ala analog 1400IU；Milter oxytocic drug consumption is the half of raun；
Fully-developed raun after screening abdominal part expands obvious, the athletic injection through oxytocic drug carries out artificial dry method and awards
Essence, its gonopore and neighbouring body surface dry towel are wiped clean, and gently squeeze ovary with finger from top to bottom on raun abdominal part top straight
Can not naturally flowing out to ovum grain, at once milter seminal fluid is clamp-oned using same method after taking ovum and use feather gentle agitation, then
Add a small amount of clear water stirring 10s, stand 2 minutes, finally add a large amount of clean water unstickings, and germ cell is poured into hatching frame
Middle water drenching hatching；
Incubating oosperm is the process that fertilized fish roe makes the smooth membrane of fry under certain artificial condition, during hatching, using day
Light lamp illuminate, and 17 DEG C of water temperature, the depth of water be 50cm, dissolved oxygen be 9mg/L, flow velocity be 2.5m/s hatchery in hatch 3 days,
Then flow velocity is down to 1.5m/s to hatch 5 days；
Germ cell membrane after hatching, enters the larvae cultivation stage；In larvae cultivation, after fry membrane, 8-10 days itself yolk is inhaled
Harvest complete, start to carry out opening feeding with artemia nauplii, after 15 days, with the Limnodrilus hoffmeisteri that minces and artifical compound feed according to
Weight proportion 1:1 mixed material is raised, and is wherein gradually lowered the consumption of Limnodrilus hoffmeisteri with the growth of small fish, after 28 days,
Fed using artifical compound feed completely.
After fry grows to 2-3 centimetre, control raising the fry density in cylinder in 1.1 ten thousand tails/m2, every morning, in
Noon and evening respectively feed once, and the standard that feeds is showed a small surplus by feedstuff and is defined, and often continuous feeding, after 7 days, stops feeding one day.
Fry enters postlarva breeding phase after growing to 3 ~ 5cm；Now fry is proceeded to big specification cultivation fish pond and carry out flowing water
Raise, as shown in figure 1, the high 130cm in fishpond pond, depth of water 70cm, fishpond flowing water pH value is 8.0；In fishpond 2, setting fountain type enters
The mouth of a river 1, fountain type water inlet is located at the normal position 6m of fishpond, directly splashes into pond in waterfall shape；This fishpond 2 is in staged
Distribution, for usual water inlet is directly accessed culturing pool for duct type, this fishpond is by high-rise tuber drainage to lower floor
Pond, the mouth of pipe is in flat, therefore, using this itself drop of cultivation platform, water is directly fallen in pond as waterfall, a side
Face can avoid big flow to enter the injury that water impacts to fry, reduces the loss of fry physical ability；On the other hand water can be increased molten
Oxygen amount, it is to avoid in breeding process, anoxia phenomenon occurs；In this fishpond, fry density is 500,000 tails/mu, and bait is using manually joining
Close feedstuff, feeding number of times is that sooner or later respectively once being satiated with food to feed is showed a small surplus as standard with feedstuff, until fry growth to 7 lis
Enhancement releasing can be used for after packing during rice.
Transport releases the fry being possessed the condition of releasing and carries out through fountain type water inlet, after oxygen supplement net training, being caught with net
It is contained in the fish bag of oxygenation after dragging for, then releases to corresponding location, using anesthetis MS-222 4mg/L, fry is carried out when releasing
Anesthesia is calm, it is to avoid postlarva mutually extrudes in response to swashing and collision causes body injures and deaths, and then raising releases survival rate.
Using the method for the present embodiment, about 2,000,000 tail short hairs schizothoracin original seed fries, warp are released to Yalongjiang River successively
Repeatedly validation trial, technique is solid, is at home and abroad pioneering, has significant economic, society and environmental ecology
Benefit.
Although being described in detail to the specific embodiment of the present invention in conjunction with specific embodiments, it is not to this
The restriction of scope of patent protection.In claims limited range, those skilled in the art is without creative work
The various modifications that can make or adjustment are still protected by this patent.
Claims (4)
1. Yalongjiang River short hairs schizothoracin artificial fecundation releases method, it is characterized in that, including：
（One）Select the Yalongjiang River short hairs schizothoracin raun physically fit, gonad development is good and milter as parent fish, using fiber crops
Liquor-saturated dose of MS-222 60 ~ 12mg/L anaesthetizes to the parent fish selecting, and the pectoral fin base portion then at parent fish injects oxytocic drug, 13
Effect 36 ~ 48 hours at～16 DEG C；Wherein, milter oxytocic drug consumption is the half of raun；Used by every kg body weight raun
Oxytocic drug is grouped into by the group of following weight：Cyprinus carpio hypophysis cerebri 1mg+ chorionic-gonadotropin hormone 16 μ g+ lutropin discharges
Hormone analogs 1400IU；
（Two）To raun just before giving birth, using dry method of artificial fertilization, and it is gently mixed with feather and makes seminal fluid and ovum mix homogeneously, then plus
Enter clear water and continue stirring 10 seconds, stand 2 minutes, then add clean water unsticking, finally put into germ cell in hatching frame
Water drenching is hatched；
（Three）Hatch above-mentioned germ cell, Spawning water temperature is 13-17 DEG C, and indoor hatching adopts fluorescent lamp lighting；
（Four）Germ cell membrane after hatching, enters the larvae cultivation stage；8-10 days after prelarva membrane, entered with artemia nauplii
Row opening feeds；After 13-15 days, by the Limnodrilus hoffmeisteri mincing and artifical compound feed according to 1:Carry out after 1 weight proportion mixing
Raise；After 25-28 days, fed using artifical compound feed completely；
（Five）After fry grows to 2-3 centimetre, control raising the fry density in cylinder in 1-1.1 ten thousand tails/m2, every morning, in
Noon and evening respectively feed once, and the standard that feeds is showed a small surplus by feedstuff and is defined, and often continuous feeding, after 7 days, stops feeding one day；
（Six）Treat that fry total length reaches 3-5 centimetre, proceed to postlarva breeding phase；Fry is proceeded in big specification cultivation fish pond and carries out
Flowing water is raised, and arranges fountain type water inlet in fishpond, and water inlet is located at 4-6m directly over fishpond, directly splashes in waterfall shape
Enter pond, water depth 70-100cm, water pH value is 7.0-8.0；Fry density is 500,000 tails/mu；Entered using artifical compound feed
Row is fed, and it is daily sooner or later secondary for feeding number of times, and the standard that feeds is showed a small surplus by feedstuff and is defined；
（Seven）After postlarva grow to total length be more than 7 centimetres after, carry out artificial releasing；When releasing, using anesthetis MS-222 20 ~ 40
It is calm that/L carries out anesthesia to fry, it is to avoid postlarva mutually extrudes in response to swashing and collision causes body injures and deaths, is released with improving
Survival rate afterwards.
2. Yalongjiang River short hairs schizothoracin artificial fecundation according to claim 1 releases method, it is characterized in that, step（One）
In, the consumption of anesthetis MS-222 is 60mg/L；After injection oxytocic drug, effect 36 hours at 15 DEG C.
3. Yalongjiang River short hairs schizothoracin artificial fecundation according to claim 1 releases method, it is characterized in that, step（Three）
In, the actual conditions of incubating oosperm is：
Will be through step（Two）Process germ cell 13 ~ 17 DEG C of water temperature, the depth of water be 50cm, dissolved oxygen be more than 7mg/L, flow velocity
For hatching in the hatchery of 2.0 ~ 2.5m/s 3 ~ 4 days, then flow velocity is down to 1.0-1.5m/s and hatches 3-5 days.
4. Yalongjiang River short hairs schizothoracin artificial fecundation according to claim 3 releases method, it is characterized in that, step（Three）
In, the actual conditions of incubating oosperm is：
Will be through step（Two）The germ cell processing 15 DEG C of water temperature, the depth of water be 50cm, dissolved oxygen be that 7.5mg/L, flow velocity are
Hatch in the hatchery of 2.0m/s 4 days, then flow velocity is down to 1.0m/s and hatches 3 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610853527.8A CN106417117A (en) | 2016-09-27 | 2016-09-27 | Yalong River schizothorax wangchiachii artificial proliferation and release method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610853527.8A CN106417117A (en) | 2016-09-27 | 2016-09-27 | Yalong River schizothorax wangchiachii artificial proliferation and release method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106417117A true CN106417117A (en) | 2017-02-22 |
Family
ID=58170356
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610853527.8A Pending CN106417117A (en) | 2016-09-27 | 2016-09-27 | Yalong River schizothorax wangchiachii artificial proliferation and release method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106417117A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109042409A (en) * | 2018-08-27 | 2018-12-21 | 吴常文 | A kind of islands and reefs area intertidal zone ladder type snail aquaculture device |
| CN110178779A (en) * | 2019-07-04 | 2019-08-30 | 水利部中国科学院水工程生态研究所 | A kind of design method of the cultivation demand of fish multiplication pouring station |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101411311A (en) * | 2008-12-05 | 2009-04-22 | 河北农业大学 | Method for producing fingerling of Trachidermus fasciatus |
| CN202664018U (en) * | 2012-05-15 | 2013-01-16 | 贵阳学院 | A fountain-type terraced giant salamander culture pond |
-
2016
- 2016-09-27 CN CN201610853527.8A patent/CN106417117A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101411311A (en) * | 2008-12-05 | 2009-04-22 | 河北农业大学 | Method for producing fingerling of Trachidermus fasciatus |
| CN202664018U (en) * | 2012-05-15 | 2013-01-16 | 贵阳学院 | A fountain-type terraced giant salamander culture pond |
Non-Patent Citations (3)
| Title |
|---|
| 李明云: "《水产经济动物增养殖学》", 31 August 2011 * |
| 赵树海 等: "长丝裂腹鱼全人工繁殖试验", 《水生态学杂志》 * |
| 邓民龙 等: "裂腹鱼人工驯养繁殖技", 《水产养殖》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109042409A (en) * | 2018-08-27 | 2018-12-21 | 吴常文 | A kind of islands and reefs area intertidal zone ladder type snail aquaculture device |
| CN110178779A (en) * | 2019-07-04 | 2019-08-30 | 水利部中国科学院水工程生态研究所 | A kind of design method of the cultivation demand of fish multiplication pouring station |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112042570B (en) | Method for efficiently cultivating and domesticating micropterus salmoides water bloom seedlings in pond greenhouse | |
| CN103444610B (en) | Artificial propagation method for acrossocheilus yunnanensis | |
| CN101103706B (en) | Reproduction method for American reeves shad artificial cultivation parent fish | |
| CN103931525A (en) | Artificial reproduction and fry breeding method for Gymnodiptychus dybowskii | |
| CN102124974A (en) | Method for artificially breeding seed of spotted maigre | |
| CN104273074A (en) | Method for cultivating inkfish fries | |
| CN105075941A (en) | Cross breeding method for eleotris oxycephala and oxyeleotris marmoratus | |
| CN104642212A (en) | Artificial breeding method for onychostoma simus | |
| CN101960996A (en) | Artificial breeding method of Scaphesthes macrolepis fry | |
| CN104170778A (en) | Large-scale common sucker breeding method capable of controlling temperature of circulating water | |
| CN106359195B (en) | A kind of offspring breeding method of vacricorhinus gerlachi | |
| CN105052782A (en) | Artificial breeding method for Triplophysa xichangensis | |
| CN101878741B (en) | Interbreeding method of hemibarbus labeo and hemibarbus maculatus | |
| CN110024722A (en) | A kind of artificial fecundation method of scavenger | |
| CN104396813A (en) | Gymnocypris przewalskii full artificial breeding method | |
| CN101647412B (en) | Method for breeding tridentiger trigonocephalus by artificial induced spawning and insemination | |
| CN100425128C (en) | Method for artificial propagation of Elopichthys bambus | |
| CN104255615B (en) | It is a kind of that soft thorn is naked splits buttocks fish artificial fecundation method | |
| CN103493767A (en) | Cross breeding method of odontobutis potamophila and odontobutis yaluensis | |
| CN105532539B (en) | A kind of mud fish fry artificial propagation breeding method | |
| CN104285855A (en) | Large-scale xenocypris davidi bleeker artificial reproduction method | |
| CN106376500B (en) | The thin squama schizothoracin artificial fecundation of Yalongjiang River releases method | |
| CN106417117A (en) | Yalong River schizothorax wangchiachii artificial proliferation and release method | |
| CN102484983A (en) | Artificial propagation method of schizothorax prenanti | |
| CN107155971A (en) | Yi Zhong Gan fishes and black carp quasi aromatic hybrid rice breeding method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170222 |
|
| RJ01 | Rejection of invention patent application after publication |