CN106258957B - A kind of roxburgh anoectochilus terminal bud is without hormone containerization cultural method - Google Patents
A kind of roxburgh anoectochilus terminal bud is without hormone containerization cultural method Download PDFInfo
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- CN106258957B CN106258957B CN201610641550.0A CN201610641550A CN106258957B CN 106258957 B CN106258957 B CN 106258957B CN 201610641550 A CN201610641550 A CN 201610641550A CN 106258957 B CN106258957 B CN 106258957B
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- Prior art keywords
- terminal bud
- roxburgh anoectochilus
- anoectochilus terminal
- culture
- containerization
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- 241000719837 Anoectochilus Species 0.000 title claims abstract description 44
- 229940088597 hormone Drugs 0.000 title claims abstract description 14
- 239000005556 hormone Substances 0.000 title claims abstract description 14
- 238000000034 method Methods 0.000 title claims abstract description 7
- 241000196324 Embryophyta Species 0.000 claims abstract description 19
- 241000233855 Orchidaceae Species 0.000 claims abstract description 8
- 238000003306 harvesting Methods 0.000 claims abstract description 8
- 238000005286 illumination Methods 0.000 claims abstract description 7
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 22
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 20
- 239000001888 Peptone Substances 0.000 claims description 10
- 108010080698 Peptones Proteins 0.000 claims description 10
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 10
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 10
- 235000019319 peptone Nutrition 0.000 claims description 10
- 229920001817 Agar Polymers 0.000 claims description 8
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 8
- 229930006000 Sucrose Natural products 0.000 claims description 8
- 239000008272 agar Substances 0.000 claims description 8
- 239000005720 sucrose Substances 0.000 claims description 8
- 239000001963 growth medium Substances 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 239000007640 basal medium Substances 0.000 claims description 4
- 238000011534 incubation Methods 0.000 abstract 1
- 230000028446 budding cell bud growth Effects 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 239000005416 organic matter Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 description 1
- 241000934230 Anoectochilus roxburghii Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 206010058314 Dysplasia Diseases 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 244000131316 Panax pseudoginseng Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000000202 analgesic effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000003630 growth substance Substances 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 230000003054 hormonal effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011785 micronutrient Substances 0.000 description 1
- 235000013369 micronutrients Nutrition 0.000 description 1
- 230000001473 noxious effect Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000010496 root system development Effects 0.000 description 1
- 230000008117 seed development Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
The present invention relates to a kind of roxburgh anoectochilus terminal bud without hormone containerization cultural method, the technical issues of roxburgh anoectochilus terminal bud is without hormone containerization culture in the prior art, incubation step are solved：(1) using the orchid tissue culture bottle of 800mL as blake bottle, 200mL culture mediums are contained in blake bottle；(2) each blake bottle is colonized the roxburgh anoectochilus terminal bud tissue culture rooted seedling of a height of 3cm~5cm of 15 young plants in step (1), and tissue culture temperature is 25 ± 2 DEG C, intensity of illumination 2000Lx, light application time 14h/d, and culture can harvest roxburgh anoectochilus terminal bud after 90 days.Field cultivation link is replaced with indoor containerization culture, its average plant height reaches 16.5cm after cultivating 90 days.
Description
Technical field
The present invention relates to roxburgh anoectochilus terminal bud without hormone containerization cultural method, belong to being trained without hormone MS culture mediums for addition organic matter
Support technical field.
Background technology
Roxburgh anoectochilus terminal bud Anoectochilus roxburghii (Wal1.) Lindl plants for orchid family Anoectochilus Blume perennial herb
Object.Roxburgh anoectochilus terminal bud be mainly distributed on the height above sea level such as China Fujian, Taiwan, Guangdong, Guangxi, Hainan, Sichuan, Guizhou and Yunnan 300~
The hilly country of 1200m.Amino acid content, activity of fighting against senium substance and micronutrient levels are above wild the West in roxburgh anoectochilus terminal bud
Ginseng, is a kind of Chinese herbal medicine being widely used.Complete stool can be used as medicine, have anti-inflammatory analgetic, nourishing yin and lessening fire, it is clearing heat and detoxicating the effect of,
It is mainly used for treating the diseases such as hypertension, diabetes, tumour, rheumatism, dysplasia of children in recent years, in Chinese medicine circle, roxburgh anoectochilus terminal bud
There are " gold grass ", the title of " king of medicine ".For roxburgh anoectochilus terminal bud in addition to medicinal, plant type is small and beautiful, and golden yellow vein is in meshy arrangement and one
The indoor appreciation treasure of the great ornamental value of kind.
The seed development of roxburgh anoectochilus terminal bud is not complete, and germination percentage is extremely low under field conditions (factors), and at present, tissue-culturing rapid propagation has become roxburgh anoectochilus terminal bud
The main means of sapling multiplication.Currently, roxburgh anoectochilus terminal bud is all by seed or stem section etc. is taken to carry out tissue cultures foundation for explant
Then the sterile rapid propagation system of roxburgh anoectochilus terminal bud, greenhouse acclimatization and transplants harvest seedling.But it is found in cultivation produces, roxburgh anoectochilus terminal bud growth ten
It is point slow, it just binds to 15cm or so, and entire growth cycle was up to 3-5 months.
If can directly be harvested without field cultivation by culture in glassware in the case where not adding hormone, can also reach
The even more than plant height and yield of field cultivation, and quality is good, can also save the production links such as hardening, transplanting, management,
Growth cycle shortens, cost reduction, and economic benefit improves.Roxburgh anoectochilus terminal bud belongs to a kind of high of roxburgh anoectochilus terminal bud production without hormone tissue cultures
The new way of low cost is imitated, is the important technical for promoting roxburgh anoectochilus terminal bud fast culture.
The content of the invention
The present invention provides roxburgh anoectochilus terminal bud without hormone containerization cultural method, and field cultivation ring is replaced with indoor containerization culture
Section, its average plant height reaches 16.5cm after cultivating 90 days.
In order to achieve the object of the present invention, technical solution specifically comprises the following steps：
(1) using the orchid tissue culture bottle of 800mL as blake bottle, 200mL culture mediums are contained in blake bottle；
(2) each blake bottle is colonized the roxburgh anoectochilus terminal bud tissue culture rooted seedling of a height of 3cm~5cm of 15 young plants, tissue culture temperature in step (1)
It spends for 25 ± 2 DEG C, intensity of illumination 2000Lx, light application time 14h/d, culture can harvest roxburgh anoectochilus terminal bud after 90 days；
The 200mL culture mediums include basal medium and growth medium；
The basal medium includes MS culture mediums and peptone；
The growth medium includes murphy juice, bean sprout juice, activated carbon, sucrose and agar strip；
The 200mL culture mediums composition includes：
The peptone of 3g/L；
The murphy juice of 150g/L；
The bean sprout juice of 200g/L；
The activated carbon of 0.5g/L；
The sucrose of 30g/L；
The agar strip of 8g/L；
Surplus is MS culture mediums.
In the present invention, the specific surface area of the activated carbon is 1000-1600m2/g。
Beneficial effects of the present invention：
1st, to be free of MS culture mediums+peptone of hormone as basic culture medium, at the same add natural organic matter murphy juice and
Bean sprout juice carries out roxburgh anoectochilus terminal bud containerization culture, and peptone, murphy juice, bean sprout juice provide sufficient nutrition for roxburgh anoectochilus terminal bud growth
Substance and trace element, the promotion without hormone regulate and control, and the roxburgh anoectochilus terminal bud product of harvesting can be used for infant without hormonal components
Production；
2nd, specific surface area is used as 1000-1600m2The activated carbon of/g has certain suction-operated, can adsorb because of plant
The noxious material that continued propagation generates, while certain dark surrounds is built, be conducive to root system development；
3rd, roxburgh anoectochilus terminal bud growth and development in containing abundant organic matter without hormone culture-medium is good, it is average after cultivating 90 days
Plant height reaches 16.5cm, and plant height improves 10% compared with the prior art, and plant is neat, healthy and strong, color depth, and yield compares same number
Tissue-cultured seedling field cultivation output increased 15%~20%；
4th, using the orchid tissue culture bottle of 800mL, it disclosure satisfy that plant fully grows, 200mL culture mediums are persistently given birth to for plant
Production provides sufficient nutrition.
Specific embodiment
Hereinafter with reference to example, the present invention is described in further detail, but the present invention is not restricted to these specific example.
Specific embodiment 1：
(1) using the orchid tissue culture bottle of 800mL as blake bottle, 200mL culture mediums are contained in blake bottle；
(2) each blake bottle is colonized the roxburgh anoectochilus terminal bud tissue culture rooted seedling of a height of 3cm~5cm of 15 young plants in step (1),
Tissue culture temperature is 25 ± 2 DEG C, intensity of illumination 2000Lx, light application time 14h/d, after cultivating 90 days
Roxburgh anoectochilus terminal bud can be harvested, roxburgh anoectochilus terminal bud plant height is average 15.9cm；
The 200mL culture mediums composition includes：
The peptone of 3g/L；
The murphy juice of 100g/L；
The bean sprout juice of 150g/L；
The activated carbon of 0.5g/L；
The sucrose of 30g/L；
The agar strip of 8g/L；
Surplus is MS culture mediums.
Specific embodiment 2：
(1) using the orchid tissue culture bottle of 800mL as blake bottle, 200mL culture mediums are contained in blake bottle；
(2) each blake bottle is colonized the roxburgh anoectochilus terminal bud tissue culture rooted seedling of a height of 3cm~5cm of 15 young plants, tissue culture temperature in step (1)
It spends for 25 ± 2 DEG C, intensity of illumination 2000Lx, light application time 14h/d, culture can harvest roxburgh anoectochilus terminal bud, roxburgh anoectochilus terminal bud strain after 90 days
A height of average 16.2cm；
The 200mL culture mediums composition includes：
The peptone of 3g/L；
The murphy juice of 130g/L；
The bean sprout juice of 180g/L；
The activated carbon of 0.5g/L；
The sucrose of 30g/L；
The agar strip of 8g/L；
Surplus is MS culture mediums.
Specific embodiment 3：
(1) using the orchid tissue culture bottle of 800mL as blake bottle, 200mL culture mediums are contained in blake bottle；
(2) each blake bottle is colonized the roxburgh anoectochilus terminal bud tissue culture rooted seedling of a height of 3cm~5cm of 15 young plants, tissue culture temperature in step (1)
It spends for 25 ± 2 DEG C, intensity of illumination 2000Lx, light application time 14h/d, culture can harvest roxburgh anoectochilus terminal bud, roxburgh anoectochilus terminal bud strain after 90 days
A height of average 16.5cm；
The 200mL culture mediums composition includes：
The peptone of 3g/L；
The murphy juice of 150g/L；
The bean sprout juice of 200g/L；
The activated carbon of 0.5g/L；
The sucrose of 30g/L；
The agar strip of 8g/L；
Surplus is MS culture mediums.
Specific embodiment 4：
(1) using the orchid tissue culture bottle of 800mL as blake bottle, 200mL culture mediums are contained in blake bottle；
(2) each blake bottle is colonized the roxburgh anoectochilus terminal bud tissue culture rooted seedling of a height of 3cm~5cm of 15 young plants, tissue culture temperature in step (1)
It spends for 25 ± 2 DEG C, intensity of illumination 2000Lx, light application time 14h/d, culture can harvest roxburgh anoectochilus terminal bud, roxburgh anoectochilus terminal bud strain after 90 days
A height of average 16.3cm；
The 200mL culture mediums composition includes：
The peptone of 3g/L；
The murphy juice of 180g/L；
The bean sprout juice of 250g/L；
The activated carbon of 0.5g/L；
The sucrose of 30g/L；
The agar strip of 8g/L；
Surplus is MS culture mediums.
Claims (2)
1. a kind of roxburgh anoectochilus terminal bud is without hormone containerization cultural method, it is characterized in that comprising the following steps：
(1) using the orchid tissue culture bottle of 800mL as blake bottle, 200mL culture mediums are contained in blake bottle；
(2) each blake bottle is colonized the roxburgh anoectochilus terminal bud tissue culture rooted seedling of a height of 3cm~5cm of 15 young plants in step (1), and tissue culture temperature is
25 ± 2 DEG C, intensity of illumination 2000Lx, light application time 14h/d, culture can harvest roxburgh anoectochilus terminal bud after 90 days；
The 200mL culture mediums include basal medium and growth medium；
The basal medium includes MS culture mediums and peptone；
The growth medium includes murphy juice, bean sprout juice, activated carbon, sucrose and agar strip；
The 200mL culture mediums composition includes：
The peptone of 3g/L；
The murphy juice of 150g/L；
The bean sprout juice of 200g/L；
The activated carbon of 0.5g/L；
The sucrose of 30g/L；
The agar strip of 8g/L；
Surplus is MS culture mediums.
2. roxburgh anoectochilus terminal bud according to claim 1 is without hormone containerization cultural method, it is characterised in that：The ratio of the activated carbon
Surface area is 1000-1600m2/g。
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| CN201610641550.0A CN106258957B (en) | 2016-08-08 | 2016-08-08 | A kind of roxburgh anoectochilus terminal bud is without hormone containerization cultural method |
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| CN106258957B true CN106258957B (en) | 2018-05-25 |
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| CN111543328B (en) * | 2020-06-19 | 2024-07-19 | 福建省中科生物股份有限公司 | Liquid tissue culture device for anoectochilus roxburghii sprouts |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101124890A (en) * | 2006-08-18 | 2008-02-20 | 上海雷允上科技发展有限公司 | Method for cultivating tissue cultured gloden line lotus seedling |
| CN102884982A (en) * | 2012-10-31 | 2013-01-23 | 贵州省亚热带作物研究所 | Hormone-free rapid propagation organic production method of anoectochilus formosanus germchit |
| CN103416305A (en) * | 2013-07-19 | 2013-12-04 | 福建省农业科学院农业生物资源研究所 | Hormone-free tissue culture and rapid propagation method of anoectochilus formosanus seedlings |
| CN103416310A (en) * | 2013-08-28 | 2013-12-04 | 叶宝鑫 | Culture medium used for roxburgh anoectochilus terminal bud organic tissue culture |
| CN105104190A (en) * | 2015-08-11 | 2015-12-02 | 广东虎形山生物科技有限公司 | Anoectochilus roxburghii tissue culture seedling method |
| CN105284609A (en) * | 2015-06-29 | 2016-02-03 | 厦门医学高等专科学校 | Method for cultivating edible hormone-free tissue culture anoectochilus formosanus |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08308412A (en) * | 1995-05-19 | 1996-11-26 | Netsutairin Saisei Gijutsu Kenkyu Kumiai | Formation of multibud body by tissue culture of dipterocarpoceae tree |
| CN101564008B (en) * | 2008-04-24 | 2011-03-23 | 中国医学科学院药用植物研究所 | Hormone-free cultivation and rapid propagation method of dendrobium candidum axenic seedlings |
| CN104186314B (en) * | 2014-08-06 | 2016-08-24 | 宁明县红枫中药材种植专业合作社 | A kind of method for culturing seedlings of Herba Anoectochili roxburghii |
| CN104429965B (en) * | 2014-12-16 | 2016-11-16 | 四川省自然资源科学研究院 | The method that high eyebrow Herba Anoectochili roxburghii seed is bred without hormone quickly tissue culture |
-
2016
- 2016-08-08 CN CN201610641550.0A patent/CN106258957B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101124890A (en) * | 2006-08-18 | 2008-02-20 | 上海雷允上科技发展有限公司 | Method for cultivating tissue cultured gloden line lotus seedling |
| CN102884982A (en) * | 2012-10-31 | 2013-01-23 | 贵州省亚热带作物研究所 | Hormone-free rapid propagation organic production method of anoectochilus formosanus germchit |
| CN103416305A (en) * | 2013-07-19 | 2013-12-04 | 福建省农业科学院农业生物资源研究所 | Hormone-free tissue culture and rapid propagation method of anoectochilus formosanus seedlings |
| CN103416310A (en) * | 2013-08-28 | 2013-12-04 | 叶宝鑫 | Culture medium used for roxburgh anoectochilus terminal bud organic tissue culture |
| CN105284609A (en) * | 2015-06-29 | 2016-02-03 | 厦门医学高等专科学校 | Method for cultivating edible hormone-free tissue culture anoectochilus formosanus |
| CN105104190A (en) * | 2015-08-11 | 2015-12-02 | 广东虎形山生物科技有限公司 | Anoectochilus roxburghii tissue culture seedling method |
Non-Patent Citations (3)
| Title |
|---|
| 适宜工厂化生产的江西野生金线莲组织培养技术研究;王莹等;《农业与技术》;20140331;第34卷(第3期);摘要、第1.1-1.2节 * |
| 金线莲的组织培养研究;范子南等;《福建师范大学学报（自然科学版）》;19971231;第13卷(第2期);第82-87页 * |
| 金线莲组织培养新体系建立及优化;黄勇;《北方园艺》;20101231(第13期);第178-179页 * |
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