CN106258957A - A kind of Herba Anoectochili roxburghii is without hormone containerization cultural method and culture medium thereof - Google Patents
A kind of Herba Anoectochili roxburghii is without hormone containerization cultural method and culture medium thereof Download PDFInfo
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- CN106258957A CN106258957A CN201610641550.0A CN201610641550A CN106258957A CN 106258957 A CN106258957 A CN 106258957A CN 201610641550 A CN201610641550 A CN 201610641550A CN 106258957 A CN106258957 A CN 106258957A
- Authority
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- Prior art keywords
- herba anoectochili
- anoectochili roxburghii
- culture medium
- containerization
- hormone
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- 239000001963 growth medium Substances 0.000 title claims abstract description 39
- 229940088597 hormone Drugs 0.000 title claims abstract description 15
- 239000005556 hormone Substances 0.000 title claims abstract description 15
- 238000000034 method Methods 0.000 title claims abstract description 6
- 241000196324 Embryophyta Species 0.000 claims abstract description 22
- 240000002514 Cymbidium ensifolium Species 0.000 claims abstract description 8
- 238000005286 illumination Methods 0.000 claims abstract description 7
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 22
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 20
- 239000001888 Peptone Substances 0.000 claims description 10
- 108010080698 Peptones Proteins 0.000 claims description 10
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 10
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 10
- 235000019319 peptone Nutrition 0.000 claims description 10
- 229920001817 Agar Polymers 0.000 claims description 8
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 8
- 229930006000 Sucrose Natural products 0.000 claims description 8
- 239000008272 agar Substances 0.000 claims description 8
- 239000005720 sucrose Substances 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 6
- 239000007640 basal medium Substances 0.000 claims description 4
- 238000011534 incubation Methods 0.000 abstract 1
- 239000003814 drug Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000012258 culturing Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 description 1
- 241000719837 Anoectochilus Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 206010058314 Dysplasia Diseases 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 244000131316 Panax pseudoginseng Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000000202 analgesic effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010924 continuous production Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000007952 growth promoter Substances 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 230000003054 hormonal effect Effects 0.000 description 1
- 239000007943 implant Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011785 micronutrient Substances 0.000 description 1
- 235000013369 micronutrients Nutrition 0.000 description 1
- 230000001473 noxious effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000010496 root system development Effects 0.000 description 1
- 230000008117 seed development Effects 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
The present invention relates to a kind of Herba Anoectochili roxburghii without hormone containerization cultural method and culture medium thereof, solve the technical problem that in prior art, Herba Anoectochili roxburghii is cultivated without hormone containerization, incubation step: (1), with the Cymbidium ensifolium (L.) Sw. tissue culture bottle of 800mL as culture bottle, contains 200mL culture medium in culture bottle;(2) in step (1), the Herba Anoectochili roxburghii group of each culture bottle field planting 15 a height of 3cm ~ 5cm of young plant trains Seedling of taking root, and group training temperature is 25 ± 2 DEG C, and intensity of illumination is 2000 Lx, and light application time is 14h/d, can gather in the crops Herba Anoectochili roxburghii after cultivating 90 days.Cultivating with indoor containerization and replace field cultivation link, after cultivating 90 days, its average plant height reaches 16.5cm.
Description
Technical field
The present invention relates to a kind of Herba Anoectochili roxburghii without hormone containerization cultural method and culture medium thereof, belong to the organic nothing of interpolation
Hormone MS culture medium culturing technical field.
Background technology
Herba Anoectochili roxburghiiAnoectochilus roxburghii(Wal1.) Lindl is that the orchid family Anoectochilus Blume perennial herb is planted
Thing.Herba Anoectochili roxburghii be mainly distributed on the height above sea level such as China Fujian, Taiwan, Guangdong, Guangxi, Hainan, Sichuan, Guizhou and Yunnan 300~
The hilly country of 1200m.In Herba Anoectochili roxburghii, amino acid content, activity of fighting against senium material and micronutrient levels are above wild the West
Ginseng, is a kind of Chinese herbal medicine being widely used.Herb can be used as medicine, and has effect of anti-inflammatory analgetic, nourishing YIN to lower pathogenic fire, heat-clearing and toxic substances removing,
It is mainly used in recent years treating the diseases such as hypertension, diabetes, tumor, rheumatism, dysplasia of children, at Chinese medicine circle, Herba Anoectochili roxburghii
There are " gold grass ", the title of " king of medicine ".Herba Anoectochili roxburghii is in addition to medicinal, and its plant type is small and exquisite attractive in appearance, and vein is golden yellow in meshy arrangement, is also one
Plant the indoor appreciation treasure of great ornamental value.
The seed development of Herba Anoectochili roxburghii is not complete, and germination percentage is extremely low under field conditions (factors), and at present, tissue-culturing rapid propagation has become Herba Anoectochili roxburghii
The Main Means of sapling multiplication.Currently, Herba Anoectochili roxburghii is all by seed or to take stem section etc. and carry out tissue culture's foundation for outer implant
The aseptic rapid propagation system of Herba Anoectochili roxburghii, booth acclimatization and transplants, then gather in the crops seedling.But find in cultivation and production, Herba Anoectochili roxburghii growth ten
Divide slowly, just bind to about 15cm, and whole growth cycle reaches 3-5 month.
If by culture in glassware in the case of without hormone, can directly gathering without field cultivation, also can reach
The even more than plant height of field cultivation and yield, and quality is good, also can save seedling exercising, transplant, the production link such as management,
Growth cycle shortens, cost reduces, and economic benefit improves.Herba Anoectochili roxburghii, without hormone tissue culture, belongs to a kind of height that Herba Anoectochili roxburghii produces
The new way of effect low cost, is the important technical promoting Herba Anoectochili roxburghii fast culture.
Summary of the invention
The present invention provides a kind of Herba Anoectochili roxburghii without hormone containerization cultural method and culture medium thereof, cultivates generation with indoor containerization
For field cultivation link, after cultivating 90 days, its average plant height reaches 16.5cm.
In order to realize the purpose of the present invention, technical scheme specifically includes following steps:
(1) with the Cymbidium ensifolium (L.) Sw. tissue culture bottle of 800mL as culture bottle, culture bottle is contained 200mL culture medium;
(2) in step (1), the Herba Anoectochili roxburghii group of each culture bottle field planting 15 a height of 3cm ~ 5cm of young plant trains Seedling of taking root, and group training temperature is
25 ± 2 DEG C, intensity of illumination is 2000Lx, and light application time is 14h/d, can gather in the crops Herba Anoectochili roxburghii after cultivating 90 days;
Described 200mL culture medium includes basal medium and growth medium;
Described basal medium includes MS culture medium and peptone;
Described growth medium includes murphy juice, bean sprout juice, activated carbon, sucrose and agar strip;
Described 200mL culture medium composition comprises:
The peptone of 3g/L;
The murphy juice of 150g/L;
The bean sprout juice of 200g/L;
The activated carbon of 0.5g/L;
The sucrose of 30g/L;
The agar strip of 8g/L;
Surplus is MS culture medium.
In the present invention, the specific surface area of described activated carbon is 1000-1600m2/g。
Beneficial effects of the present invention:
1, culture medium based on the MS culture medium+peptone without hormone, adds natural organic matter murphy juice and bean sprout simultaneously
Juice carries out containerization cultivation to Herba Anoectochili roxburghii, and peptone, murphy juice, bean sprout juice are the nutrient substance that Herba Anoectochili roxburghii growth provides abundance
And trace element, it is not necessary to the promotion regulation and control of hormone, the Herba Anoectochili roxburghii product gathered does not contains hormonal components, may be used for infant product
Produce;
2, using specific surface area is 1000-1600m2The activated carbon of/g, has certain adsorption, can adsorb because plant continues
The noxious substance that growth produces, builds certain dark surrounds, beneficially root system development simultaneously;
3, Herba Anoectochili roxburghii containing abundant organic without hormone culture-medium in growth promoter well, its average plant height after cultivating 90 days
Arriving 16.5cm, plant height relatively prior art improves 10%, and plant is neat, healthy and strong, color depth, same amount of group of yield ratio
Seedlings cultivating is at the output increased 15% ~ 20% of field cultivation;
4, the Cymbidium ensifolium (L.) Sw. tissue culture bottle of 800mL is used, it is possible to meeting plant and fully grow, 200mL culture medium is that plant continuous production carries
For sufficient nutrition.
Detailed description of the invention
Hereinafter with reference to example, the present invention is described in further detail, but the invention is not restricted to these instantiations.
Specific embodiment 1:
(1) with the Cymbidium ensifolium (L.) Sw. tissue culture bottle of 800mL as culture bottle, culture bottle is contained 200mL culture medium;
(2) in step (1), the Herba Anoectochili roxburghii group of each culture bottle field planting 15 a height of 3cm ~ 5cm of young plant trains Seedling of taking root, and group training temperature is
25 ± 2 DEG C, intensity of illumination is 2000 Lx, and light application time is 14h/d, can gather in the crops Herba Anoectochili roxburghii after cultivating 90 days, and Herba Anoectochili roxburghii plant height is
Average 15.9cm;
Described 200mL culture medium composition comprises:
The peptone of 3g/L;
The murphy juice of 100g/L;
The bean sprout juice of 150g/L;
The activated carbon of 0.5g/L;
The sucrose of 30g/L;
The agar strip of 8g/L;
Surplus is MS culture medium.
Specific embodiment 2:
(1) with the Cymbidium ensifolium (L.) Sw. tissue culture bottle of 800mL as culture bottle, culture bottle is contained 200mL culture medium;
(2) in step (1), the Herba Anoectochili roxburghii group of each culture bottle field planting 15 a height of 3cm ~ 5cm of young plant trains Seedling of taking root, and group training temperature is
25 ± 2 DEG C, intensity of illumination is 2000 Lx, and light application time is 14h/d, can gather in the crops Herba Anoectochili roxburghii after cultivating 90 days, and Herba Anoectochili roxburghii plant height is
Average 16.2cm;
Described 200mL culture medium composition comprises:
The peptone of 3g/L;
The murphy juice of 130g/L;
The bean sprout juice of 180g/L;
The activated carbon of 0.5g/L;
The sucrose of 30g/L;
The agar strip of 8g/L;
Surplus is MS culture medium.
Specific embodiment 3:
(1) with the Cymbidium ensifolium (L.) Sw. tissue culture bottle of 800mL as culture bottle, culture bottle is contained 200mL culture medium;
(2) in step (1), the Herba Anoectochili roxburghii group of each culture bottle field planting 15 a height of 3cm ~ 5cm of young plant trains Seedling of taking root, and group training temperature is
25 ± 2 DEG C, intensity of illumination is 2000 Lx, and light application time is 14h/d, can gather in the crops Herba Anoectochili roxburghii after cultivating 90 days, and Herba Anoectochili roxburghii plant height is
Average 16.5cm;
Described 200mL culture medium composition comprises:
The peptone of 3g/L;
The murphy juice of 150g/L;
The bean sprout juice of 200g/L;
The activated carbon of 0.5g/L;
The sucrose of 30g/L;
The agar strip of 8g/L;
Surplus is MS culture medium.
Specific embodiment 4:
(1) with the Cymbidium ensifolium (L.) Sw. tissue culture bottle of 800mL as culture bottle, culture bottle is contained 200mL culture medium;
(2) in step (1), the Herba Anoectochili roxburghii group of each culture bottle field planting 15 a height of 3cm ~ 5cm of young plant trains Seedling of taking root, and group training temperature is
25 ± 2 DEG C, intensity of illumination is 2000 Lx, and light application time is 14h/d, can gather in the crops Herba Anoectochili roxburghii after cultivating 90 days, and Herba Anoectochili roxburghii plant height is
Average 16.3cm;
Described 200mL culture medium composition comprises:
The peptone of 3g/L;
The murphy juice of 180g/L;
The bean sprout juice of 250g/L;
The activated carbon of 0.5g/L;
The sucrose of 30g/L;
The agar strip of 8g/L;
Surplus is MS culture medium.
Claims (3)
1. Herba Anoectochili roxburghii is without a hormone containerization cultural method, it is characterized in that comprising the following steps:
(1) with the Cymbidium ensifolium (L.) Sw. tissue culture bottle of 800mL as culture bottle, culture bottle is contained 200mL culture medium;
(2) in step (1), the Herba Anoectochili roxburghii group of each culture bottle field planting 15 a height of 3cm ~ 5cm of young plant trains Seedling of taking root, and group training temperature is
25 ± 2 DEG C, intensity of illumination is 2000 Lx, and light application time is 14h/d, can gather in the crops Herba Anoectochili roxburghii after cultivating 90 days.
Herba Anoectochili roxburghii the most according to claim 1 is without hormone containerization culture medium, it is characterised in that: described 200mL culture medium
Including basal medium and growth medium;
Described basal medium includes MS culture medium and peptone;
Described growth medium includes murphy juice, bean sprout juice, activated carbon, sucrose and agar strip;
Described 200mL culture medium composition comprises:
The peptone of 3g/L;
The murphy juice of 150g/L;
The bean sprout juice of 200g/L;
The activated carbon of 0.5g/L;
The sucrose of 30g/L;
The agar strip of 8g/L;
Surplus is MS culture medium.
Herba Anoectochili roxburghii the most according to claim 2 is without hormone containerization culture medium, it is characterised in that: the ratio table of described activated carbon
Area is 1000-1600m2/g。
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CN201610641550.0A CN106258957B (en) | 2016-08-08 | 2016-08-08 | A kind of roxburgh anoectochilus terminal bud is without hormone containerization cultural method |
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CN106258957B CN106258957B (en) | 2018-05-25 |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111543328A (en) * | 2020-06-19 | 2020-08-18 | 福建省中科生物股份有限公司 | Gold thread lotus bud seedling liquid tissue culture apparatus |
Citations (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH08308412A (en) * | 1995-05-19 | 1996-11-26 | Netsutairin Saisei Gijutsu Kenkyu Kumiai | Formation of multibud body by tissue culture of dipterocarpoceae tree |
CN101124890A (en) * | 2006-08-18 | 2008-02-20 | 上海雷允上科技发展有限公司 | Method for cultivating tissue cultured gloden line lotus seedling |
CN101564008A (en) * | 2008-04-24 | 2009-10-28 | 中国医学科学院药用植物研究所 | Hormone-free cultivation and rapid propagation method of dendrobium candidum axenic seedlings |
CN102884982A (en) * | 2012-10-31 | 2013-01-23 | 贵州省亚热带作物研究所 | Hormone-free rapid propagation organic production method of anoectochilus formosanus germchit |
CN103416305A (en) * | 2013-07-19 | 2013-12-04 | 福建省农业科学院农业生物资源研究所 | Hormone-free tissue culture and rapid propagation method of anoectochilus formosanus seedlings |
CN103416310A (en) * | 2013-08-28 | 2013-12-04 | 叶宝鑫 | Culture medium used for roxburgh anoectochilus terminal bud organic tissue culture |
CN104186314A (en) * | 2014-08-06 | 2014-12-10 | 宁明县红枫中药材种植专业合作社 | Anoectochilus formosanus seedling culturing method |
CN104429965A (en) * | 2014-12-16 | 2015-03-25 | 四川省自然资源科学研究院 | Hormone-free rapid tissue culture and propagation method of Emei roxburgh anoectochilus terminal bud seeds |
CN105104190A (en) * | 2015-08-11 | 2015-12-02 | 广东虎形山生物科技有限公司 | Anoectochilus roxburghii tissue culture seedling method |
CN105284609A (en) * | 2015-06-29 | 2016-02-03 | 厦门医学高等专科学校 | Method for cultivating edible hormone-free tissue culture anoectochilus formosanus |
-
2016
- 2016-08-08 CN CN201610641550.0A patent/CN106258957B/en not_active Expired - Fee Related
Patent Citations (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH08308412A (en) * | 1995-05-19 | 1996-11-26 | Netsutairin Saisei Gijutsu Kenkyu Kumiai | Formation of multibud body by tissue culture of dipterocarpoceae tree |
CN101124890A (en) * | 2006-08-18 | 2008-02-20 | 上海雷允上科技发展有限公司 | Method for cultivating tissue cultured gloden line lotus seedling |
CN101564008A (en) * | 2008-04-24 | 2009-10-28 | 中国医学科学院药用植物研究所 | Hormone-free cultivation and rapid propagation method of dendrobium candidum axenic seedlings |
CN102884982A (en) * | 2012-10-31 | 2013-01-23 | 贵州省亚热带作物研究所 | Hormone-free rapid propagation organic production method of anoectochilus formosanus germchit |
CN103416305A (en) * | 2013-07-19 | 2013-12-04 | 福建省农业科学院农业生物资源研究所 | Hormone-free tissue culture and rapid propagation method of anoectochilus formosanus seedlings |
CN103416310A (en) * | 2013-08-28 | 2013-12-04 | 叶宝鑫 | Culture medium used for roxburgh anoectochilus terminal bud organic tissue culture |
CN104186314A (en) * | 2014-08-06 | 2014-12-10 | 宁明县红枫中药材种植专业合作社 | Anoectochilus formosanus seedling culturing method |
CN104429965A (en) * | 2014-12-16 | 2015-03-25 | 四川省自然资源科学研究院 | Hormone-free rapid tissue culture and propagation method of Emei roxburgh anoectochilus terminal bud seeds |
CN105284609A (en) * | 2015-06-29 | 2016-02-03 | 厦门医学高等专科学校 | Method for cultivating edible hormone-free tissue culture anoectochilus formosanus |
CN105104190A (en) * | 2015-08-11 | 2015-12-02 | 广东虎形山生物科技有限公司 | Anoectochilus roxburghii tissue culture seedling method |
Non-Patent Citations (3)
Title |
---|
王莹等: "适宜工厂化生产的江西野生金线莲组织培养技术研究", 《农业与技术》 * |
范子南等: "金线莲的组织培养研究", 《福建师范大学学报(自然科学版)》 * |
黄勇: "金线莲组织培养新体系建立及优化", 《北方园艺》 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111543328A (en) * | 2020-06-19 | 2020-08-18 | 福建省中科生物股份有限公司 | Gold thread lotus bud seedling liquid tissue culture apparatus |
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