CN105993871B - A kind of fast seedling-cultivating method promoting the high germination rate of Radix Notoginseng using branch acremonium - Google Patents
A kind of fast seedling-cultivating method promoting the high germination rate of Radix Notoginseng using branch acremonium Download PDFInfo
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- CN105993871B CN105993871B CN201610323271.XA CN201610323271A CN105993871B CN 105993871 B CN105993871 B CN 105993871B CN 201610323271 A CN201610323271 A CN 201610323271A CN 105993871 B CN105993871 B CN 105993871B
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- radix notoginseng
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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Abstract
The invention discloses a kind of fast seedling-cultivating methods for promoting the high germination rate of Radix Notoginseng using branch acremonium, comprising the following steps: Spawn incubation, sowing, breeding management, sprouts transplanting at seed treatment.The present invention is more traditional to be compared with water pouring seed, and the germination rate of Panax notoginseng seeds improves 20% or more, and germination rate can be up to 90% or more, seedling raise period can shorten 10-20 days, and the seedling cultivated is healthy and strong neat, and growth is fast, the time that nursery can be saved has a very important significance the plantation of Radix Notoginseng.
Description
Technical field
The present invention relates to method for culturing seedlings fields, and in particular to a kind of to promote the quick of the high germination rate of Radix Notoginseng using branch acremonium
Method for culturing seedlings.
Background technique
Three seventh is that Araliaceae Panax herbaceos perennial, there is the title of " invaluable ", " southern part of the country god grass ", root, stem, flower
It can be used as medicine, be the distinctive rare traditional Chinese medicine in Yunnan.Present pharmaceutical research show Radix Notoginseng hematological system, cardiovascular system,
Nervous system, immune system, metabolic system and anti-inflammatory, anti-aging, Kang Zhong liu method have pharmacological activity and unique curative effect.Three
Seven implantation methods are usually seed collecting, preservation, nursery, transplanting, harvesting.Currently used nursery means are that moisturizing will save after harvesting
Seed be sowed at soil, under conditions of keeping temperature appropriate, moisture content, Panax notoginseng seeds is made to sprout to obtain seedling.Due to Radix Notoginseng
With apparent allelopathy effect, therefore sowing germination percentage is lower on the spot, seedling raise period is longer, less use.In the prior art,
Radix Notoginseng nursery is embedded in soil or substrate seedling after generally drying refrigerator cold-storage using the substrate seedling and disinfection that are directly embedded in humidity,
Although can improve the germination rate of Panax notoginseng seeds compared with seeding and seedling raising under natural environment, there is also germination rates can only achieve 50
~70%, sprouting time is up to 45~60d, seriously constrains the development of notoginseng planting industry.And in recent years by various extreme
Weather influence so that Panax notoginseng seeds germination percentage and seedling percent substantially reduce under the conditions of the prior art, therefore is studied such as
What, which quickly improves Panax notoginseng seeds sprouting, is of great significance.
Summary of the invention
In view of the deficiencies of the prior art, promote the quick of the high germination rate of Radix Notoginseng using branch acremonium the present invention provides a kind of
Method for culturing seedlings can be improved the germination rate of Panax notoginseng seeds, shorten seedling raise period.
In order to achieve the above object, the present invention is achieved by the following technical programs:
A kind of fast seedling-cultivating method promoting the high germination rate of Radix Notoginseng using branch acremonium, comprising the following steps:
1) Spawn incubation: the branch acremonium strain (AcremoniumyunnanensisY saved in 28 DEG C of incubators is taken
.L.DuMHYAU3001, depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center, address: Beijing
The institute 3 of city, North Star West Road, Chaoyang District 1, preservation date: on 04 21st, 2016, deposit number are as follows: CGMCC No.12374),
Be inoculated into PDA culture medium, 28 DEG C dark culturing 2 weeks, collect mycelia and the spore of growth, add water and stir uniformly, obtain containing bacterium
The aqueous solution of silk and spore；
2) seed treatment: the Radix Notoginseng plant of selection well-grown, no disease and pests harm harvests Radix Notoginseng Red mature fruit, removes pericarp, obtain
Panax notoginseng seeds, disinfection, are tedded；
3) sow: the Panax notoginseng seeds after selecting the disinfection of full grains, being seeded into humidity is 25-30% containing perlite
It in the seedling medium of quartz sand, is irrigated with the aqueous solution containing mycelia and spore of step 1), later every 12-15 days with containing bacterium
The aqueous solution of silk and spore pours once, repeats to pour twice, natural lighting is sprouted；
4) breeding management: the humidity for after planting keeping the seedling medium containing perlite and quartz sand is 25-30%, and temperature is
22-26 DEG C, illumination ventilation；
5) sprout transplanting: after planting 40-45d Panax notoginseng seeds start to sprout, 50-60d Radix Notoginseng seedling average height in 5-7cm,
About 55-65d or so can be transplanted.
Preferably, in aqueous solution of the step 1) containing mycelia and spore mycelia and spore concentration OD600For 0.2-0.3.
Preferably, disinfection is to impregnate Panax notoginseng seeds 8-10min using 10% liquor natrii hypochloritis in the step 2).
Preferably, the moisture content that rear Panax notoginseng seeds are tedded in the step 2) is 10-12%.
Preferably, step 3) the depth of planting control is in 1.0-1.5cm.
The invention has the advantages that: the rudiments that the branch acremonium in the present invention not only promotes Panax notoginseng seeds, also promotion Radix Notoginseng children
The growth of seedling, improves the survival rate of seedling, more traditional to pour seed with water and compare, the germination rate of Panax notoginseng seeds improve 20% with
On, germination rate can be up to 90% or more, and seedling raise period can shorten 10-20 days, and the seedling cultivated is healthy and strong neat, and growth is fast, can
The time for saving nursery, the plantation of Radix Notoginseng is had a very important significance.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention for those of ordinary skill in the art without creative efforts, can be with
It obtains other drawings based on these drawings.
Fig. 1: experimental group and control group sow Radix Notoginseng growth of seedling situation after 55d, and wherein A is control group, and B is experimental group.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention
In attached drawing, technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described embodiment is
A part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art
Every other embodiment obtained without creative efforts, shall fall within the protection scope of the present invention.
Embodiment:
A kind of fast seedling-cultivating method promoting the high germination rate of Radix Notoginseng using branch acremonium, comprising the following steps:
1) Spawn incubation: branch acremonium strain (the Acremonium yunnanensis saved in 28 DEG C of incubators is taken
Y.L.Du MHYAU3001, save number be 12374), to be inoculated into PDA culture medium, 28 DEG C dark culturing 2 weeks, collect give birth to
Long mycelia and spore is added water and stirs evenly, obtain the concentration OD of mycelia and spore600For the aqueous solution of 0.2-0.3；
2) seed treatment: the Radix Notoginseng plant of selection well-grown, no disease and pests harm harvests Radix Notoginseng Red mature fruit, removes pericarp, obtain
Panax notoginseng seeds impregnate Panax notoginseng seeds 8-10min disinfection with 10% liquor natrii hypochloritis, take out, tedding makes its moisture content 10-
12%；
3) sow: the Panax notoginseng seeds after selecting the disinfection of full grains, being seeded into humidity is 25-30% containing perlite
In the seedling medium of quartz sand, depth of planting 1.0-1.5cm is irrigated with the aqueous solution containing mycelia and spore of step 1),
It was poured later every 12-15 days once, repeats to pour twice, natural lighting is sprouted；
4) breeding management: the humidity for after planting keeping the seedling medium containing perlite and quartz sand is 25-30%, and temperature is
22-26 DEG C, illumination ventilation.
5) sprout transplanting: after planting 40-45d Panax notoginseng seeds start to sprout, 50-60d Radix Notoginseng seedling average height in 5-7cm,
About 55-65d or so can be transplanted.
Prepare the mycelia of acremonium containing branch and spore concentration OD600For 0.25 aqueous solution, nursery conduct is carried out by the above process
Experimental group, in addition control group replaces branch acremonium after planting to irrigate seedling medium in Panax notoginseng seeds disinfection using water, remaining is educated
Seedling step and rearing condition are identical as experimental group.From figure 1 it appears that control group growth of seedling speed is significantly faster than that control
Group, after planting 40-45d Panax notoginseng seeds start to sprout experimental group, and 50-60d Radix Notoginseng seedling average height can be high in 5-7cm, germination rate
Up to 90%；After planting 50-60d Panax notoginseng seeds just start to sprout control group, and germination rate average value only has 71%.
The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although with reference to the foregoing embodiments
Invention is explained in detail, those skilled in the art should understand that: it still can be to aforementioned each implementation
Technical solution documented by example is modified or equivalent replacement of some of the technical features；And these modification or
Replacement, the spirit and scope for technical solution of various embodiments of the present invention that it does not separate the essence of the corresponding technical solution.
Claims (5)
1. a kind of fast seedling-cultivating method for promoting the high germination rate of Radix Notoginseng using branch acremonium, which comprises the following steps:
1) Spawn incubation: branch acremonium strain (the Acremonium yunnanensis saved in 28 DEG C of incubators is taken
Y.L.Du MHYAU3001, deposit number are as follows: CGMCC No.12374), it is inoculated into PDA culture medium, in 28 DEG C of dark culturings 2
Week mycelia and the spore of growth are collected, adds water and stirs uniformly, obtain the aqueous solution containing mycelia and spore；
2) seed treatment: the Radix Notoginseng plant of selection well-grown, no disease and pests harm harvests Radix Notoginseng Red mature fruit, removes pericarp, obtain Radix Notoginseng
Seed, disinfection, is tedded；
3) sow: the Panax notoginseng seeds after selecting the disinfection of full grains, being seeded into humidity is 25-30% containing perlite and stone
In the seedling medium of sand, irrigated with the aqueous solution containing mycelia and spore of step 1), later every 12-15 days with containing mycelia and
The aqueous solution of spore pours once, repeats to pour twice, natural lighting is sprouted；
4) breeding management: the humidity for after planting keeping the seedling medium containing perlite and quartz sand is 25-30%, temperature 22-
26 DEG C, illumination ventilation；
5) sprout transplanting: after planting 40-45d Panax notoginseng seeds start to sprout, and 50-60d Radix Notoginseng seedling average height is in 5-7cm, 55-
65d transplanting.
2. promoting the fast seedling-cultivating method of the high germination rate of Radix Notoginseng using branch acremonium as described in claim 1, which is characterized in that
The concentration OD of mycelia and spore in aqueous solution of the step 1) containing mycelia and spore600For 0.2-0.3.
3. promoting the fast seedling-cultivating method of the high germination rate of Radix Notoginseng using branch acremonium as described in claim 1, which is characterized in that
Disinfection is to impregnate Panax notoginseng seeds 8-10min using 10% liquor natrii hypochloritis in the step 2).
4. promoting the fast seedling-cultivating method of the high germination rate of Radix Notoginseng using branch acremonium as described in claim 1, which is characterized in that
The moisture content that rear Panax notoginseng seeds are tedded in the step 2) is 10-12%.
5. promoting the fast seedling-cultivating method of the high germination rate of Radix Notoginseng using branch acremonium as described in claim 1, which is characterized in that
Step 3) the depth of planting control is in 1.0-1.5cm.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610323271.XA CN105993871B (en) | 2016-05-16 | 2016-05-16 | A kind of fast seedling-cultivating method promoting the high germination rate of Radix Notoginseng using branch acremonium |
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| CN201610323271.XA CN105993871B (en) | 2016-05-16 | 2016-05-16 | A kind of fast seedling-cultivating method promoting the high germination rate of Radix Notoginseng using branch acremonium |
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| CN105993871A CN105993871A (en) | 2016-10-12 |
| CN105993871B true CN105993871B (en) | 2019-10-22 |
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| CN201610323271.XA Expired - Fee Related CN105993871B (en) | 2016-05-16 | 2016-05-16 | A kind of fast seedling-cultivating method promoting the high germination rate of Radix Notoginseng using branch acremonium |
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| CN109497091B (en) * | 2018-12-27 | 2021-04-20 | 云南农业大学 | Biological control preparation with nematode killing function and preparation method thereof |
| CN109721403A (en) * | 2018-12-29 | 2019-05-07 | 云南农业大学 | A kind of microbial culture medium and preparation method thereof promoting soybean growth |
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| CN101444230A (en) * | 2008-12-26 | 2009-06-03 | 南京师范大学 | Method for producing novel lance atractylodes rhizome inoculation agent |
| CN103444387A (en) * | 2012-05-31 | 2013-12-18 | 黄九荣 | Panax notoginseng planting method |
| CN103583108A (en) * | 2013-11-01 | 2014-02-19 | 云南农业大学 | Pseudo-ginseng seedling growing method |
| CN104447127A (en) * | 2014-12-17 | 2015-03-25 | 成都新朝阳作物科学有限公司 | Soil conditioner for resisting pseudo-ginseng continuous cropping diseases as well as preparation and application thereof |
| CN105191542A (en) * | 2015-09-16 | 2015-12-30 | 云南三七科技资源药物研究有限公司 | High-germination-rate fast seedling growing method for pseudo-ginseng seeds |
| CN105340708A (en) * | 2015-11-10 | 2016-02-24 | 云南师范大学 | Industrialized seedling production method of panax notoginseng |
| CN105379611A (en) * | 2015-10-14 | 2016-03-09 | 中国科学院昆明植物研究所 | Rapid propagation method for panax bipinnatifidus seed |
| CN105462847A (en) * | 2015-12-23 | 2016-04-06 | 广西大学 | Application of sophora tonkinensis endophytic fungus TRXY-46 in preventing and controlling panax notoginseng root rot |
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2016
- 2016-05-16 CN CN201610323271.XA patent/CN105993871B/en not_active Expired - Fee Related
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101444230A (en) * | 2008-12-26 | 2009-06-03 | 南京师范大学 | Method for producing novel lance atractylodes rhizome inoculation agent |
| CN103444387A (en) * | 2012-05-31 | 2013-12-18 | 黄九荣 | Panax notoginseng planting method |
| CN103583108A (en) * | 2013-11-01 | 2014-02-19 | 云南农业大学 | Pseudo-ginseng seedling growing method |
| CN104447127A (en) * | 2014-12-17 | 2015-03-25 | 成都新朝阳作物科学有限公司 | Soil conditioner for resisting pseudo-ginseng continuous cropping diseases as well as preparation and application thereof |
| CN105191542A (en) * | 2015-09-16 | 2015-12-30 | 云南三七科技资源药物研究有限公司 | High-germination-rate fast seedling growing method for pseudo-ginseng seeds |
| CN105379611A (en) * | 2015-10-14 | 2016-03-09 | 中国科学院昆明植物研究所 | Rapid propagation method for panax bipinnatifidus seed |
| CN105340708A (en) * | 2015-11-10 | 2016-02-24 | 云南师范大学 | Industrialized seedling production method of panax notoginseng |
| CN105462847A (en) * | 2015-12-23 | 2016-04-06 | 广西大学 | Application of sophora tonkinensis endophytic fungus TRXY-46 in preventing and controlling panax notoginseng root rot |
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Effective date of registration: 20200106 Address after: 650201 Yunnan Province, Panlong District of Kunming City gold black drop cable slope No. 132 Co-patentee after: Du Yunlong Patentee after: Yunnan Agricultural University Address before: 650201, room 1, unit 24, building 666, No. 502, Xiaokang Road, Wuhua District, Yunnan, Kunming Patentee before: Du Yunlong |
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