CN106215232B - Wound antibacterial healing-promoting dressing and preparation method thereof - Google Patents
Wound antibacterial healing-promoting dressing and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0028—Polypeptides; Proteins; Degradation products thereof
- A61L26/0047—Specific proteins or polypeptides not covered by groups A61L26/0033 - A61L26/0042
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0004—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing inorganic materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0014—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials obtained by reactions only involving carbon-to-carbon unsaturated bonds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0023—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/008—Hydrogels or hydrocolloids
Abstract
The invention discloses a wound bacteriostasis and healing promotion dressing, which comprises the effective components of a paste of a silk fibroin raw material, a bioactive glass raw material, a chitosan raw material and a polyvinyl alcohol raw material which are uniformly mixed. The preparation method of the dressing is also disclosed, the silk fibroin solution and the bioactive glass powder are mixed uniformly, then the chitosan solution and the polyvinyl alcohol solution are added and stirred into a mixed solution, the wound dressing material which is difficult to heal is prepared by repeated freeze thawing, and the moisture permeability, the water absorption, the water retention and the antibacterial performance of the obtained dressing can be controlled by adjusting the proportion among the raw materials of the silk fibroin solution, the chitosan solution, the polyvinyl alcohol solution and the bioactive glass, so that the dressing material can meet the requirement of promoting wound healing; the preparation method has the advantages of mild reaction conditions, simple process and low raw material cost, and is suitable for industrial large-scale production.
Description
Technical Field
the invention relates to the technical field of wound dressing preparations, in particular to a wound bacteriostasis healing-promoting dressing and a preparation method thereof.
Background
With the increasing standard of living, wound treatment and care are receiving attention. For some wounds which are difficult to heal, the common bandaging treatment is often difficult to heal, and special means are needed. The wounds difficult to heal generally comprise chronic difficult wounds such as pressure sores, diabetic feet, arteriovenous ulcers, malignant tumors and the like, surgical wounds such as traffic wounds, wound acute wounds, burn and plastic wounds and the like, and surgical wounds such as cardiac stent operations, fat liquefaction, abscess incision and drainage and the like. The wound is difficult to heal, and some systemic diseases cause the damage of immune cells of a human body; the old and the infirm, malnutrition and the like cause insufficient cell regeneration capacity; wound infection, inflammatory reaction, etc. The root causes of this are poor collagen synthesis and impaired fibroblast metabolism. Some clinicians are also dedicated to the treatment of wounds which are difficult to heal, and the usage amount of wound dressings in some hospitals can reach more than 70 ten thousand times per year, so that the demand is extremely high.
The wound dressings which are clinically used at present mainly comprise traditional dressings and novel wet dressings. The traditional dressings, namely gauze, iodoform gauze and the like, are used for dressing change treatment by taking the traditional principle of keeping the wound surface dry as a guide principle, and have no healing promotion effect. The novel wet dressing includes a covering type outer dressing represented by a transparent film type dressing; inner layer-packed dressings represented by alginate dressings and wound gels; the antibacterial dressing represented by silver ion dressing and the like can create a moist environment favorable for cell growth by taking moist healing as a principle, and the dressing closed or semi-closed wound can keep the wound at a constant temperature, prevent bacteria and external granular foreign matters from invading, reduce the infection chance of the wound, can not generate mechanical damage when the dressing is replaced, and promote the wound to heal. However, the characteristics and permeability of the drug for absorbing the exudate in a humid environment are poor, and finally, many infection situations appear, which becomes a medical problem.
In recent years, third generation dressings, known as the future technology type, have become a hot spot for development. The method is characterized in that: the intelligent automatic wound moistening degree adjusting device has the performance of intelligently and automatically adjusting the wound moistening degree, so that tissues and cells are in the optimal growth environment; the dressing has the components capable of providing nutrition for cell and tissue growth and inhibiting bacteria reproduction. The wound dressing with the vacuum reservoir, which is invented by D, Fenke and the like, which is most representative, can drain wound exudate in time, keep the wound from being infected, and the wound surface material has a nutritional function, is quick in wound healing, but is extremely high in price, and is difficult for patients to bear.
therefore, the development of a dressing product with third generation dressing features and reduced cost is a problem that needs to be solved.
Disclosure of Invention
Aiming at the defects of the prior art, the technical problems to be solved by the invention are as follows: how to provide a wound bacteriostasis healing promoting dressing which has good air permeability, superior water absorption, water retention and bacteriostasis performance and low cost and can better promote the healing of a wound and a preparation method thereof, so that the dressing is particularly suitable for the wound which is difficult to heal.
in order to solve the technical problems, the invention adopts the following technical scheme:
A preparation method of a wound antibacterial healing-promoting dressing is characterized by uniformly mixing silk fibroin solution and bioactive glass powder, adding chitosan solution and polyvinyl alcohol solution, stirring into a mixed solution, and repeatedly freezing and thawing to obtain the wound antibacterial healing-promoting dressing.
The wound bacteriostasis and healing promotion dressing prepared by the method adopts four substances of silk fibroin solution, bioactive glass powder and chitosan solution and polyvinyl alcohol solution as raw materials, wherein the silk fibroin is natural high molecular protein extracted from cocoon shells and contains 18 amino acids, and glycine (Gly), alanine (Ala) and serine (Ser) account for about 80 percent of the total composition. The aggregation structure of silk fibroin includes two major portions of crystalline and amorphous. The fibroin has the special performance of amphoteric charge, has no toxicity, good biocompatibility and biodegradability, has the function of promoting the growth of epidermal cells, and can provide nutrient substances such as amino acid and the like for tissues at wounds; the bioactive glass as inorganic biological synthetic material has unique surface activity and specific chemical composition, especially calcium and phosphorus plasma deposit to form one rack-shaped hydroxyapatite layer, and this results in great specific surface area, favorable cell adhesion growth, favorable nutrients and oxygen intake, favorable metabolite exhaust, favorable blood vessel and nerve growth and thus wound healing. The composition of the epithelial growth factor of the cells can be continuously induced, the natural epithelial growth factor with complete biological function of the patient is provided for the local part of the wound surface, and the important effect on the rapid healing of the wound surface is achieved; the chitosan has excellent performances of relieving pain, stopping bleeding, promoting wound healing, reducing scars, inhibiting bacteria, good biocompatibility, biodegradability and the like, and is very suitable to be used as a raw material of wound dressing; polyvinyl alcohol hydrogel (PVA) is a water-soluble polymer with a three-dimensional cross-linked network structure, has hydrophilicity, softness, mildness and good biocompatibility, and is widely applied to the field of biomedicine. Meanwhile, the characteristic of repeated freeze thawing and thawing of PVA is utilized during preparation, when the uniform PVA aqueous solution is cooled to be lower than the freezing point, the macromolecular chains are extruded to the outside of ice (solid water), phase separation is carried out, the local concentration of the molecular chains in the macromolecular phases is increased, and the molecular chains are sufficiently close to each other to form hydrogen bonds to crystal nuclei. When the frozen body is thawed, further crystallization occurs, and the generated PVA microcrystals construct a firm three-dimensional network structure, and the space is filled with water. The PVA hydrogel obtained by the method has high water content and is transparent as a whole.
As an optimization, the method specifically comprises the following steps:
(1) Dissolving silk fibroin powder in deionized water to prepare a silk fibroin solution with the mass volume percentage concentration of 2.0-5.0%;
Dissolving chitosan in a dilute acid solution to prepare a chitosan solution with the mass volume percentage concentration of 1.5-2.5%;
dissolving polyvinyl alcohol in deionized water at 90-98 ℃ to prepare a polyvinyl alcohol solution with the mass volume percentage concentration of 5.0-10.0%;
Obtaining bioactive glass powder;
(2) at room temperature, uniformly mixing the silk fibroin solution obtained in the step (1) with bioactive glass, adding a chitosan solution and a polyvinyl alcohol solution, stirring to form a mixed solution, and uniformly stirring (about 30 min), wherein the volume ratio of the silk fibroin solution to the chitosan solution to the polyvinyl alcohol solution is 7-2: 63-18: 27-72; the proportion of the added bioactive glass is 0.5-1.0% of mass volume percentage concentration; and pouring the mixed solution into a mould, freezing for many times and then thawing to obtain the dressing material.
Thus, by adopting the steps, the silk fibroin is easily dissolved in the deionized water, the chitosan is dissolved by adopting dilute acetic acid, the chitosan can be completely dissolved, and the polyvinyl alcohol can be dissolved only in a high-temperature water bath. Therefore, the raw materials can be better promoted to complete dissolving and mixing.
As optimization, the mixed material freezing and thawing operation in the step (2) is as follows: firstly, pouring the mixed solution into a mold, freezing for 8-10 h at-20 ℃, then placing at room temperature until unfreezing, and repeating the steps for three times.
By freezing and thawing in this way, the polyvinyl alcohol can be phase separated and crystallized to form PVA microcrystals and construct a firm three-dimensional network structure.
As optimization, the preparation method of the fibroin powder in the step (1) comprises the following steps: soaking cocoon shell in ether for at least 48 hr, washing with distilled water, and drying to remove wax; soaking in alcohol for at least 48 hr, washing with distilled water, and drying to remove partial organic substances and impurities; boiling the cleaned cocoon shells of the cocoons in a Na2CO3 solution with the mass percent of 0.5% for about 3 hours, and removing sericin; washing the cocoon shells without sericin with deionized water, drying in the air, and placing in a CaCl2 volume ratio: C2H5 OH: dissolving fibroin completely in H2O =1:2:8 solution in water bath at 80 deg.C to obtain transparent fibroin solution, dialyzing, vacuum filtering, concentrating, and freeze drying to obtain pure fibroin protein powder.
The silk fibroin powder is prepared by the method, so that the purity of the silk fibroin can be improved, and the cost is reduced.
As an optimization, the bioactive glass powder in the step (1) is obtained according to the following preparation method: the preparation method comprises the steps of uniformly mixing raw materials for forming the bioactive glass according to a certain proportion, stirring the mixture in a closed manner for 1 hour at room temperature, aging the mixture for 3 days at room temperature to obtain uniform sol, putting the uniform sol into a 75 ℃ blast drying oven for 1 day to obtain uniform wet gel, putting the wet gel into a 150 ℃ blast drying oven to obtain dry gel powder, putting the dry gel into a muffle furnace, maintaining the temperature at 700 ℃ for 2 hours, and taking the dry gel out after cooling to obtain the bioactive glass powder.
In the preparation method of the bioactive glass powder, the raw materials and the proportion in the existing preparation method of the bioactive glass can be adopted, and ethyl orthosilicate, triethyl phosphate and calcium nitrate tetrahydrate can be preferably adopted according to the proportion of 12.5 parts by mass: 8.5 parts by mass: 1.46 parts by mass of raw materials, so that the catalyst has the characteristics of low synthesis temperature and good uniformity. In addition, the bioactive glass powder prepared by combining the sol-gel method principle adopted by the preparation method can have nano-scale micropores, huge specific surface area, higher chemical activity and adsorption characteristics.
And (3) further optimizing, wherein the dilute acid in the step (1) is dilute acetic acid, and the concentration is 0.5-1.5% (V/V). Therefore, the chitosan can be better dissolved, and the dilute acetic acid is not easy to volatilize.
And (3) as further optimization, the bioactive glass powder obtained by grinding and sieving in the step (1) is the bioactive glass powder with the particle size of 75-100 mu m. Thus, the mixing effect can be better protected, and the full play of the medicinal components can be ensured when in use.
The invention also discloses a wound bacteriostasis and healing promotion dressing which is characterized in that the active ingredients are paste which comprises the evenly mixed silk fibroin raw material, bioactive glass raw material, chitosan raw material and polyvinyl alcohol raw material.
Wherein the bioactive glass raw material, the silk fibroin raw material, the chitosan raw material and the polyvinyl alcohol raw material are in a mass part ratio of 0.5-1: 1-2: 2-9: 8-3. By adopting the proportion, experiments prove that the medicinal effect can reach the best. Specifically, the dressing with the proportion can ensure that the water permeability is 2132-2850 g/(m2 d), the optimal water permeability can reach 2460g/(m2 d), the optimal water permeability is closest to the ideal value of 2500 g/(m2 d) of the medical dressing, and simultaneously, the water absorption rate and the water retention rate respectively reach 23.70 g/g and 8.10 g/g, and the dressing can absorb wound exudate and keep the wound moist.
Further, the wound bacteriostasis healing promoting dressing is prepared by adopting the preparation method of the wound dressing.
The wound dressing has the beneficial effects that the wound dressing has good antibacterial activity and avoids wound infection. The wound dressing prepared by the invention has good water vapor permeability, water absorption and water retention. The wound dressing obtained by the invention has better healing promotion performance. The preparation method has the advantages.
In conclusion, the obtained wound bacteriostasis healing promoting dressing has the advantages of good air permeability, superior water absorption rate, water retention rate and bacteriostasis performance, low cost and capability of better promoting wound healing, and is particularly suitable for wounds which are difficult to heal. Meanwhile, the preparation method has the advantages of mild reaction conditions, simple process and low raw material cost.
Drawings
Fig. 1 is a macroscopic picture of the wound dressing material prepared in example 5.
Fig. 2 is an XRD pattern of the bioactive glass prepared.
Fig. 3 is a bacteriostatic map of dressing leach liquor of the wound dressing prepared in example 5.
fig. 4 is a diagram of wound healing conditions of the wound dressing, the sodium alginate dressing, the bioactive glass powder and the medical gauze group prepared in example 5 at each time point after the operation of the diabetic rat.
Fig. 5 is a graph of the healing time of the wound dressing prepared in example 5, the existing sodium alginate dressing, the bioactive glass powder alone and the medical gauze group corresponding to the wound surface.
Fig. 6 is a graph of the wound healing rate at each time phase point of the wound surface of the wound dressing prepared in example 5, the existing sodium alginate dressing, the single bioactive glass powder and the medical gauze group.
Detailed Description
The effects of the present invention will be further verified with reference to the drawings and the detailed description.
the specific implementation mode is as follows: a wound antibacterial healing-promoting dressing is prepared by the following steps:
(1) Dissolving silk fibroin powder in deionized water to prepare a silk fibroin solution with the mass volume percentage concentration of 2.0-5.0%;
Dissolving chitosan in a dilute acid solution to prepare a chitosan solution with the mass volume percentage concentration of 1.5-2.5%;
Dissolving polyvinyl alcohol in deionized water at 90-98 ℃ to prepare a polyvinyl alcohol solution with the mass volume percentage concentration of 5.0-10.0%;
obtaining bioactive glass powder;
(2) At room temperature, uniformly mixing the silk fibroin solution obtained in the step (1) with bioactive glass, adding a chitosan solution and a polyvinyl alcohol solution, stirring to form a mixed solution, and uniformly stirring, wherein the volume ratio of the silk fibroin solution to the chitosan solution to the polyvinyl alcohol solution is 7-2: 63-18: 27-72; the proportion of the added bioactive glass is 0.5-1.0% of mass volume percentage concentration; and pouring the mixed solution into a mould, freezing for many times and then thawing to obtain the dressing material.
Wherein, the operations of freezing and thawing the mixed material in the step (2) are as follows: firstly, pouring the mixed solution into a mold, freezing for 8-10 h at-20 ℃, then placing at room temperature until unfreezing, and repeating the steps for three times.
wherein the preparation method of the fibroin powder in the step (1) comprises the following steps: soaking cocoon shell in ether for at least 48 hr, washing with distilled water, and drying to remove wax; soaking in alcohol for at least 48 hr, washing with distilled water, and drying to remove partial organic substances and impurities; boiling the cleaned cocoon shells of the cocoons in a Na2CO3 solution with the mass percent of 0.5% for about 3 hours, and removing sericin; washing the cocoon shells without sericin with deionized water, drying in the air, and placing in a CaCl2 volume ratio: C2H5 OH: dissolving fibroin completely in H2O =1:2:8 solution in water bath at 80 deg.C to obtain transparent fibroin solution, dialyzing, vacuum filtering, concentrating, and freeze drying to obtain pure fibroin protein powder.
Wherein, the bioactive glass powder in the step (1) is obtained according to the following preparation method: the preparation method comprises the steps of uniformly mixing raw materials for forming the bioactive glass according to a certain proportion, stirring the mixture in a closed manner for 1 hour at room temperature, aging the mixture for 3 days at room temperature to obtain uniform sol, putting the uniform sol into a 75 ℃ blast drying oven for 1 day to obtain uniform wet gel, putting the wet gel into a 150 ℃ blast drying oven to obtain dry gel powder, putting the dry gel into a muffle furnace, maintaining the temperature at 700 ℃ for 2 hours, and taking the dry gel out after cooling to obtain the bioactive glass powder. Wherein, the raw materials and the proportion for preparing the bioactive glass adopt tetraethoxysilane, triethyl phosphate and calcium nitrate tetrahydrate in 12.5 parts by mass: 8.5 parts by mass: 1.46 parts by mass of the raw materials.
wherein the dilute acid in the step (1) is dilute acetic acid, and the concentration is 0.5-1.5% (V/V).
Wherein the bioactive glass powder obtained by grinding and sieving in the step (1) is 75-100 mu m in particle size.
The invention is further examined with reference to the accompanying drawings and representative examples in which different parameters are selected.
Example 1, the preparation method of the wound bacteriostatic healing-promoting dressing obtained in this example is the same as that of the above embodiment, except that the parameters of the respective range values are selected as follows: in the step 1, the mass volume percentage concentration of the fibroin solution is 5%; the mass volume percentage concentration of the chitosan solution is 1.5 percent; the mass volume percentage concentration of the polyvinyl alcohol solution is 5 percent; in step 2, the volume ratio of the silk fibroin solution, the chitosan solution and the polyvinyl alcohol solution is 7: 63: 27; the mass volume percentage concentration of the added bioactive glass is 0.5%.
example 2, the preparation method of the wound bacteriostatic healing-promoting dressing obtained in this example is the same as that of the above embodiment, except that the parameters of the respective range values are selected as follows: in the step 1, the mass volume percentage concentration of the fibroin solution is 3%; the mass volume percentage concentration of the chitosan solution is 2 percent; the mass volume percentage concentration of the polyvinyl alcohol solution is 6 percent; in step 2, the volume ratio of the silk fibroin solution, the chitosan solution and the polyvinyl alcohol solution is 6: 54: 36. the mass volume percentage concentration of the added bioactive glass is 0.5%.
Example 3, the preparation method of the wound bacteriostatic healing-promoting dressing obtained in this example is the same as that of the above embodiment, except that the parameters of the respective range values are selected as follows: in the step 1, the mass volume percentage concentration of the fibroin solution is 4%; the mass volume percentage concentration of the chitosan solution is 2.5 percent; the mass volume percentage concentration of the polyvinyl alcohol solution is 8 percent; in step 2, the volume ratio of the silk fibroin solution, the chitosan solution and the polyvinyl alcohol solution is 5: 45: 45. the mass volume percentage concentration of the added bioactive glass is 1 percent
Example 4, the preparation method of the wound bacteriostatic healing-promoting dressing obtained in this example is the same as that of the above embodiment, except that the parameters of the respective range values are selected as follows: in the step 1, the mass volume percentage concentration of the fibroin solution is 2.5 percent; the mass volume percentage concentration of the chitosan solution is 2 percent; the mass volume percentage concentration of the polyvinyl alcohol solution is 9 percent; in step 2, the volume ratio of the silk fibroin solution, the chitosan solution and the polyvinyl alcohol solution is 4: 36: 54. the mass volume percentage concentration of the added bioactive glass is 1 percent
Example 5, the preparation method of the wound bacteriostatic healing-promoting dressing obtained in this example is the same as that of the above embodiment, except that the parameters of the respective range values are selected as follows: in the step 1, the mass volume percentage concentration of the fibroin solution is 2%; the mass volume percentage concentration of the chitosan solution is 2 percent; the mass volume percentage concentration of the polyvinyl alcohol solution is 10 percent; in step 2, the volume ratio of the silk fibroin solution, the chitosan solution and the polyvinyl alcohol solution is 3: 27: 63. the mass volume percentage concentration of the added bioactive glass is 0.5 percent
Example 6, the preparation method of the wound bacteriostatic healing-promoting dressing obtained in this example is the same as that of the above embodiment, except that the parameters of the respective range values are selected as follows: in the step 1, the mass volume percentage concentration of the fibroin solution is 2%; the mass volume percentage concentration of the chitosan solution is 1.5 percent; the mass volume percentage concentration of the polyvinyl alcohol solution is 10 percent; in step 2, the volume ratio of the silk fibroin solution, the chitosan solution and the polyvinyl alcohol solution is 2: 18: 72. the mass volume percentage concentration of the added bioactive glass is 1 percent
The wound bacteriostasis healing promoting dressing prepared in the above embodiments is subjected to physical component detection and physical property detection.
through physical component detection, in the dressings prepared in six groups of embodiments, the mass parts of the bioactive glass raw material, the silk fibroin raw material, the chitosan raw material and the polyvinyl alcohol raw material are all 0.5-1: 1-2: 2-9: 8-3 in the proportion range.
according to the physical property detection results shown in the table I, the wound dressings obtained in the six embodiments have the water absorption range of 16.03-33.50 g/g, the water retention range of 6.01-11.40 g/g and the water vapor permeability range of 2132-2850 g/(m2 d), so that the wound bacteriostasis and healing promotion dressings obtained in the embodiments can achieve a good medicinal effect range. The water and air permeability of the wound dressing in the embodiment 5 can reach 2460g/(m2 d), is most close to the ideal value of 2500 g/(m2 d) of the medical dressing, simultaneously, the water absorption rate and the water retention rate respectively reach 23.70 g/g and 8.10 g/g, and can absorb the wound seepage and keep the wound moist; example 5 is the most effective dressing.
TABLE 1
| Examples | Water absorption (g/g) | Water retention rate (g/g) | 2water vapor permeability g/(m 2. d) |
| 1 | 30.45 | 10.15 | 2205 |
| 2 | 28.14 | 9.03 | 2300 |
| 3 | 16.03 | 6.01 | 2850 |
| 4 | 19.52 | 7.32 | 2611 |
| 5 | 23.70 | 8.10 | 2460 |
| 6 | 33.50 | 11.40 | 2132 |
The medicinal effect of the wound dressing prepared in example 5 was further verified by experimental tests.
Fig. 1 is a macro-topography of the dressing prepared in example 5, which shows its transparency and water-containing properties, and can be seen to have better formability.
FIG. 2 shows the intermediate products obtained in the above examples: the XRD pattern of the bioactive glass shows that the bioactive glass presents broad, dispersed and sharp diffraction peaks, which indicate that the bioactive glass is amorphous or amorphous inorganic solid particles. Wherein the bond angles of Si-O-Si and P-O-P, Si-O-P bonds are varied within a certain range and do not have fixed bond angles and lattice spacings, so that the amorphous substance has a short-range ordered and long-range disordered structure, and the amorphous substance is mainly characterized by an amorphous state.
fig. 3 is a bacteriostatic map obtained by studying bacteriostatic properties of the leaching liquor of the dressing of example 5, from which it can be seen that the leaching liquor of the dressing has stronger bacteriostatic activity on escherichia coli, staphylococcus aureus and pseudomonas aeruginosa, and the higher the concentration of the leaching liquor of the material is, the stronger the bacteriostatic activity is. On the one hand, the amino group (NH 2 +) in the chitosan solution can be combined with the bacterial cell wall to form a negative electricity environment, so that the integrity of the bacterial cell wall is damaged, and the bacteria are further damaged until the bacteria die; on the other hand, some amino groups of the silk fibroin can adsorb bacteria, interfere the normal life activities of the bacteria and generate corresponding bacteriostatic activity; on the other hand, the biological active glass may have an inhibitory effect on bacteria, and documents show that the biological active glass can locally form an alkaline environment and can play a certain bacteriostatic effect. In summary, the material leaching liquor has higher bacteriostatic activity on escherichia coli than staphylococcus aureus and pseudomonas aeruginosa, which may be related to the structure and drug resistance of bacteria, the escherichia coli belongs to gram-negative bacteria, and the cell wall is in a two-dimensional structure and is thinner; the staphylococcus aureus belongs to gram-positive bacteria, and the cell wall of the staphylococcus aureus is in a three-dimensional structure and is thick; pseudomonas aeruginosa belongs to pseudomonas and is a gram-negative bacterium with strong drug resistance which is common clinically.
Fig. 4 is a diagram of wound healing conditions of the dressing, the sodium alginate dressing, the bioactive glass powder and the medical gauze group in example 5 at each time point after the diabetic rat operation, and it can be seen through visual observation of the wound that at 2d, a part of granulation tissues are formed at the wound of the sodium alginate dressing group, and a layer of white substance is generated on the wound surface of the wound dressing group difficult to heal. And 4d, the wound surface of the wound dressing group difficult to heal is reduced, and the other two groups do not have obvious change. And 8d, the wound surface of the wound dressing group difficult to heal is obviously reduced, granulation tissues are full of the wound surface, the color of the wound surface is lightened, the granulation tissues are also formed in the sodium alginate group, the wound surface is reduced, and part of the granulation tissues are generated in the medical gauze group. And when 10 days, most of the regenerated epithelial tissues of the wound dressing group difficult to heal cover the wound surface, the wound surface of the sodium alginate group is obviously reduced, and the wound surface of the medical gauze group is not obviously reduced. And 12d, the wound dressing group with the wound difficult to heal completely covers the wound surface by the new epithelial tissue, the wound is completely healed, the wound surface of the sodium alginate group is reduced but not completely healed, the medical gauze group has granulation tissue, and the wound surface is reduced to a certain extent.
Fig. 5 is a diagram of the healing time of the postoperative wound of a diabetic rat by the dressing, the sodium alginate dressing, the bioactive glass powder and the medical gauze group in example 5, and it can be seen from the healing time of the wound (fig. 5) that the average healing time of the difficult-to-heal dressing group is (11.2 ± 0.5) d, the sodium alginate dressing group is (16.1 ± 0.67) d, and the medical gauze group is (20.5 ± 1.2) d, and the difficult-to-heal dressing group has a significant difference (p < 0.05) compared with the medical gauze group, and the healing time of the difficult-to-heal dressing group is obviously shorter than that of the sodium alginate dressing group (p < 0.05).
Fig. 6 is a graph of the healing rate of the wound surface after the operation of the diabetic rat by the dressing, the sodium alginate dressing, the bioactive glass powder and the medical gauze group in example 5, and the healing rate of the difficult-to-heal dressing group at the same time phase point is obviously higher than that of the sodium alginate dressing group, the bioactive glass group and the medical gauze group (p is less than 0.05), so that the healing is faster.
Claims (2)
1. A preparation method of a wound bacteriostatic healing-promoting dressing is characterized in that a silk fibroin solution and bioactive glass powder are uniformly mixed, then a chitosan solution and a polyvinyl alcohol solution are added, the mixture is stirred into a mixed solution, and then repeated freezing and thawing are carried out to obtain a paste dressing; the bioactive glass raw material, the silk fibroin raw material, the chitosan raw material and the polyvinyl alcohol raw material are 0.5-1: 1-2: 2-9: 8-3;
the method specifically comprises the following steps:
(1) Dissolving silk fibroin powder in deionized water to prepare a sericin solution with the mass volume percentage concentration of 2.0-5.0%;
dissolving chitosan in a dilute acid solution to prepare a chitosan solution with the mass volume percentage concentration of 1.5-2.5%;
Dissolving polyvinyl alcohol in deionized water at 90-98 ℃ to prepare a polyvinyl alcohol solution with the mass volume percentage concentration of 5.0-10.0%;
Obtaining bioactive glass powder;
(2) At room temperature, uniformly mixing the silk fibroin solution obtained in the step (1) with bioactive glass, adding a chitosan solution and a polyvinyl alcohol solution, stirring to form a mixed solution, and uniformly stirring, wherein the volume ratio of the silk fibroin solution to the chitosan solution to the polyvinyl alcohol solution is 7-2: 63-18: 27-72; the proportion of the added bioactive glass is 0.5-1.0% of mass volume percentage concentration; pouring the mixed solution into a mould, freezing for many times and then thawing to obtain the dressing material;
The specific operations of freezing and thawing the mixed material in the step (2) are as follows: firstly, pouring the mixed solution into a mold, freezing for 8-10 h at-20 ℃, then placing at room temperature until unfreezing, and repeating the steps for three times;
the bioactive glass powder obtained by grinding and sieving in the step (1) is 75-100 mu m in particle size;
The bioactive glass powder in the step (1) is obtained according to the following preparation method: the preparation method comprises the steps of uniformly mixing raw materials for forming the bioactive glass according to a certain proportion, stirring the mixture in a closed manner for 1 hour at room temperature, aging the mixture for 3 days at room temperature to obtain uniform sol, putting the uniform sol into a 75 ℃ blast drying oven for 1 day to obtain uniform wet gel, putting the wet gel into a 150 ℃ blast drying oven to obtain dry gel powder, putting the dry gel into a muffle furnace, maintaining the temperature at 700 ℃ for 2 hours, and taking the dry gel out after cooling to obtain the bioactive glass powder.
2. A wound bacteriostasis and healing promotion dressing, which is characterized by being prepared by the preparation method of the wound bacteriostasis and healing promotion dressing of claim 1.
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