CN106176973A - A kind of ultrasonic extracting method of Chinese medicine composition - Google Patents
A kind of ultrasonic extracting method of Chinese medicine composition Download PDFInfo
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Abstract
The present invention provides the ultrasonic extracting method of a kind of Chinese medicine composition, described Chinese medicine composition comprises the Radix Astragali and Fructus Ligustri Lucidi, and the method can improve astragaloside in Chinese medicine composition, calycosin glucoside, the content of specnuezhenside, shortens the production cycle, reduce production cost, improve production efficiency.
Description
Technical field
The present invention relates to a kind of Radix Rehmanniae and belong to the medicinal preparation of Scrophulariaceae Dicotyledoneae Angiospermae, particularly a kind of Chinese medicine composition
Ultrasonic extracting method.
Background technology
The Radix Astragali, has another name called continuous stilbene.Herbaceos perennial, high 50-100 centimetre.Main root is plump, wooden, normal branch, canescence.Stem is upright, and top is many
Branch, has thin rib, by white pubescence.Perennial herb is high 50-100 centimetre.Originate in the ground such as the Inner Mongol, Shanxi, Gansu, Heilungkiang.It has enhancing
Body's immunity, protect the liver, diuresis, defying age, anti-stress, blood pressure lowering and wide antibacterial action.
Fructus Ligustri Lucidi, has another name called Fructus Ligustri Lucidi reality, Fructus Ilicis Purpureae, wax tree, Mus Chinese catalpa, for the dry mature fruit of Oleaceae plants Fructus Ligustri Lucidi Ligustrum lucidum Ait..
These product are avette, oval or kidney shape, and long 6～8.5mm, diameter 3.5～5.5mm.The black purple in surface or grey black, shrinkage is uneven, and base portion has carpopodium
Trace or tool Constellation calyx and short stalk.Body is light.Outer peel is thin, and mesocarp is relatively soft, is easily peeled, and endocarp is wooden, yellowish-brown, the vertical rib of tool, breaks rear seed and leads to
It is often 1, kidney shape, atropurpureus, oiliness.Odorless, sweet in the mouth, micro-bitterness.Primary in Yangtze River in China basin and south various places, Henan, Shaanxi, Gansu
Etc. ground.
Body resistance-strengthening Zhenqi prescription is made up of the Radix Astragali, Fructus Ligustri Lucidi, and it can improve immune function of human body, protection bone marrow and adrenal cortex function；Treat various
It is deficient that disease causes；Coordinate operation, lonizing radiation, chemotherapy, promote the recovery of normal function.
The patent of invention that number of patent application 200310122216.7, denomination of invention body resistance-strengthening Zhenqi medicament preparation improve discloses a kind of body resistance-strengthening Zhenqi prescription
Extracting method.The method can extract the effective ingredient such as astragaloside, oleanolic acid in the Radix Astragali, Fructus Ligustri Lucidi, but its astragaloside extracted, Mao Ruiyi
Flavone glucoside, specnuezhenside content relatively low, the production cycle is longer, and production cost is higher, and production efficiency is relatively low.
Summary of the invention
The present invention provides the ultrasonic extracting method of a kind of Chinese medicine composition, and it can improve astragaloside in Chinese medicine composition, calycosin glucose
Glycosides, the content of specnuezhenside, shorten the production cycle, reduces production cost, improves production efficiency.
In order to reach above-mentioned advantage, the present invention specifically have employed following technical scheme.
The ultrasonic extracting method of a kind of Chinese medicine composition, it comprises the steps: to weigh according to the Radix Astragali 2 weight portion, Fructus Ligustri Lucidi 1 weight portion；Will
Each medicated powder is broken for coarse granule, and coarse granule mixes, and obtains coarse granule mixture；It is for the first time 5～35 times of water, supersonic frequencies by coarse granule mixture at solvent
Rate is reflux, extract, 0.5～6 hours under conditions of 20～50kHz, filters, obtains first-time filtrate and a filter cake；For the second time by a filter cake molten
Agent is 5～35 times of water, and supersonic frequency is reflux, extract, 0.5～6 hours under conditions of 20～50kHz, filters, obtains secondary filtrate, abandons secondary filter
Cake；First-time filtrate and secondary filtrate are merged, obtains amalgamation liquid；In above-mentioned amalgamation liquid, add 60-95% ethanol, filter, obtain merging filter cake, abandon conjunction
And filtrate；By above-mentioned merging filtration cakes torrefaction, obtain Chinese medicine composition pressed powder.
The ultrasonic extracting method of Chinese medicine composition as mentioned above, it comprises the steps: to weigh according to the Radix Astragali 2 weight portion, Fructus Ligustri Lucidi 1 weight portion；
By broken for each medicated powder for coarse granule, coarse granule mixes, and obtains coarse granule mixture；It is for the first time 10 times of water, supersonic frequencies by coarse granule mixture at solvent
Rate is reflux, extract, 1 hour under conditions of 25kHz, filters, obtains first-time filtrate and a filter cake；Filter cake is 10 times at solvent by second time
Water, supersonic frequency is reflux, extract, 1 hour under conditions of 25kHz, filters, obtains secondary filtrate, abandon secondary filter cake；By first-time filtrate and secondary
Filtrate merges, and obtains amalgamation liquid；In above-mentioned amalgamation liquid, add 60-95% ethanol, filter, obtain merging filter cake, abandon merging filtrate；Above-mentioned merging is filtered
Cookies is dry, obtains Chinese medicine composition pressed powder.
The ultrasonic extracting method of Chinese medicine composition as mentioned above, it comprises the steps: to weigh according to the Radix Astragali 2 weight portion, Fructus Ligustri Lucidi 1 weight portion；
By broken for each medicated powder for coarse granule, coarse granule mixes, and obtains coarse granule mixture；It is for the first time 15 times of water, supersonic frequencies by coarse granule mixture at solvent
Rate is reflux, extract, 2 hours under conditions of 30kHz, filters, obtains first-time filtrate and a filter cake；Filter cake is 15 times at solvent by second time
Water, supersonic frequency is reflux, extract, 2 hours under conditions of 30kHz, filters, obtains secondary filtrate, abandon secondary filter cake；By first-time filtrate and secondary
Filtrate merges, and obtains amalgamation liquid；In above-mentioned amalgamation liquid, add 60-95% ethanol, filter, obtain merging filter cake, abandon merging filtrate；Above-mentioned merging is filtered
Cookies is dry, obtains Chinese medicine composition pressed powder.
The ultrasonic extracting method of Chinese medicine composition as mentioned above, it comprises the steps: to weigh according to the Radix Astragali 2 weight portion, Fructus Ligustri Lucidi 1 weight portion；
By broken for each medicated powder for coarse granule, coarse granule mixes, and obtains coarse granule mixture；It is for the first time 20 times of water, supersonic frequencies by coarse granule mixture at solvent
Rate is reflux, extract, 3 hours under conditions of 35kHz, filters, obtains first-time filtrate and a filter cake；Filter cake is 20 times at solvent by second time
Water, supersonic frequency is reflux, extract, 3 hours under conditions of 35kHz, filters, obtains secondary filtrate, abandon secondary filter cake；By first-time filtrate and secondary
Filtrate merges, and obtains amalgamation liquid；In above-mentioned amalgamation liquid, add 60-95% ethanol, filter, obtain merging filter cake, abandon merging filtrate；Above-mentioned merging is filtered
Cookies is dry, obtains Chinese medicine composition pressed powder.
The ultrasonic extracting method of Chinese medicine composition as mentioned above, it comprises the steps: to weigh according to the Radix Astragali 2 weight portion, Fructus Ligustri Lucidi 1 weight portion；
By broken for each medicated powder for coarse granule, coarse granule mixes, and obtains coarse granule mixture；It is for the first time 25 times of water, supersonic frequencies by coarse granule mixture at solvent
Rate is reflux, extract, 4 hours under conditions of 40kHz, filters, obtains first-time filtrate and a filter cake；Filter cake is 25 times at solvent by second time
Water, supersonic frequency is reflux, extract, 4 hours under conditions of 40kHz, filters, obtains secondary filtrate, abandon secondary filter cake；By first-time filtrate and secondary
Filtrate merges, and obtains amalgamation liquid；In above-mentioned amalgamation liquid, add 60-95% ethanol, filter, obtain merging filter cake, abandon merging filtrate；Above-mentioned merging is filtered
Cookies is dry, obtains Chinese medicine composition pressed powder.
The ultrasonic extracting method of Chinese medicine composition as mentioned above, it comprises the steps: to weigh according to the Radix Astragali 2 weight portion, Fructus Ligustri Lucidi 1 weight portion；
By broken for each medicated powder for coarse granule, coarse granule mixes, and obtains coarse granule mixture；It is for the first time 30 times of water, supersonic frequencies by coarse granule mixture at solvent
Rate is reflux, extract, 5 hours under conditions of 45kHz, filters, obtains first-time filtrate and a filter cake；Filter cake is 30 times at solvent by second time
Water, supersonic frequency is reflux, extract, 5 hours under conditions of 45kHz, filters, obtains secondary filtrate, abandon secondary filter cake；By first-time filtrate and secondary
Filtrate merges, and obtains amalgamation liquid；In above-mentioned amalgamation liquid, add 60-95% ethanol, filter, obtain merging filter cake, abandon merging filtrate；Above-mentioned merging is filtered
Cookies is dry, obtains Chinese medicine composition pressed powder.
There is advantages that it can improve astragaloside in Chinese medicine composition, calycosin glucoside, the containing of specnuezhenside
Amount, shortens the production cycle, reduces production cost, improves production efficiency.
Detailed description of the invention
Below in conjunction with detailed description of the invention, the present invention is described in further details.
In following embodiment, weight portion is g or kg.
Embodiment 1
Weigh according to the Radix Astragali 2 weight portion, Fructus Ligustri Lucidi 1 weight portion；
By Fructus Ligustri Lucidi alcohol reflux, backflow filters, is evaporated to the clear paste that relative density is 1.05 (50 DEG C) after filtrate recycling ethanol,
Add suitable quantity of water under stirring, stand, standby；
The Fructus Ligustri Lucidi medicinal residues boiling leached, decoction liquor filters, is evaporated to the clear paste that relative density is 1.12 (50 DEG C), adds ethanol and make to contain
Alcohol amount reaches 30%, stirs evenly, and filters, drying precipitate after standing, standby；
By Radix Astragali boiling, decoction liquor filters, is evaporated to the clear paste that relative density is 1.12 (50 DEG C), adds ethanol and make alcohol content reach 30%,
Stirring evenly, stand, filter, solids ethanol in proper amount is washed, and filters, and solids is dried, standby, and filtrate, washing liquid merge, and subtract after reclaiming ethanol
Pressure is concentrated into the clear paste that relative density is 1.18 (50 DEG C), filters, then by filtrate by macroporous adsorptive resins, with suitable quantity of water washing resin bed,
Again with ethanol elution, collect ethanol elution, reclaim concentrating under reduced pressure after ethanol, be dried；
Dried object mixing above steps extracted, is ground into medicated powder.
Embodiment 2
Weigh according to the Radix Astragali 2 weight portion, Fructus Ligustri Lucidi 1 weight portion；By broken for each medicated powder for coarse granule, coarse granule mixes, and obtains coarse granule mixture；
Be for the first time reflux, extract, 15 minutes under conditions of 2.5 times of water, supersonic frequency are 15kHz by coarse granule mixture at solvent, filter, once
Filtrate and a filter cake；Filter cake is 2.5 times of water at solvent by second time, and supersonic frequency is reflux, extract, 15 minutes under conditions of 15kHz,
Filter, obtain secondary filtrate, abandon secondary filter cake；First-time filtrate and secondary filtrate are merged, obtains amalgamation liquid；60-95% is added in above-mentioned amalgamation liquid
Ethanol, filters, obtains merging filter cake, abandon merging filtrate；By above-mentioned merging filtration cakes torrefaction, obtain Chinese medicine composition pressed powder.
Embodiment 3
Weigh according to the Radix Astragali 2 weight portion, Fructus Ligustri Lucidi 1 weight portion；By broken for each medicated powder for coarse granule, coarse granule mixes, and obtains coarse granule mixture；
Be for the first time reflux, extract, 0.5 hour under conditions of 5 times of water, supersonic frequency are 20kHz by coarse granule mixture at solvent, filter, once
Filtrate and a filter cake；Filter cake is reflux, extract, 0.5 hour under conditions of 5 times of water, supersonic frequency are 20kHz at solvent by second time,
Filter, obtain secondary filtrate, abandon secondary filter cake；First-time filtrate and secondary filtrate are merged, obtains amalgamation liquid；60-95% is added in above-mentioned amalgamation liquid
Ethanol, filters, obtains merging filter cake, abandon merging filtrate；By above-mentioned merging filtration cakes torrefaction, obtain Chinese medicine composition pressed powder.
Embodiment 4
Weigh according to the Radix Astragali 2 weight portion, Fructus Ligustri Lucidi 1 weight portion；By broken for each medicated powder for coarse granule, coarse granule mixes, and obtains coarse granule mixture；
Be for the first time reflux, extract, 1 hour under conditions of 10 times of water, supersonic frequency are 25kHz by coarse granule mixture at solvent, filter, once filter
Liquid and a filter cake；Filter cake is reflux, extract, 1 hour under conditions of 10 times of water, supersonic frequency are 25kHz at solvent by second time, filters,
Obtain secondary filtrate, abandon secondary filter cake；First-time filtrate and secondary filtrate are merged, obtains amalgamation liquid；60-95% ethanol, mistake is added in above-mentioned amalgamation liquid
Filter, obtains merging filter cake, abandons merging filtrate；By above-mentioned merging filtration cakes torrefaction, obtain Chinese medicine composition pressed powder.
Embodiment 5
Weigh according to the Radix Astragali 2 weight portion, Fructus Ligustri Lucidi 1 weight portion；By broken for each medicated powder for coarse granule, coarse granule mixes, and obtains coarse granule mixture；
Be for the first time reflux, extract, 2 hours under conditions of 15 times of water, supersonic frequency are 30kHz by coarse granule mixture at solvent, filter, once filter
Liquid and a filter cake；Filter cake is reflux, extract, 2 hours under conditions of 15 times of water, supersonic frequency are 30kHz at solvent by second time, filters,
Obtain secondary filtrate, abandon secondary filter cake；First-time filtrate and secondary filtrate are merged, obtains amalgamation liquid；60-95% ethanol, mistake is added in above-mentioned amalgamation liquid
Filter, obtains merging filter cake, abandons merging filtrate；By above-mentioned merging filtration cakes torrefaction, obtain Chinese medicine composition pressed powder.
Embodiment 6
Weigh according to the Radix Astragali 2 weight portion, Fructus Ligustri Lucidi 1 weight portion；By broken for each medicated powder for coarse granule, coarse granule mixes, and obtains coarse granule mixture；
Be for the first time reflux, extract, 3 hours under conditions of 20 times of water, supersonic frequency are 35kHz by coarse granule mixture at solvent, filter, once filter
Liquid and a filter cake；Filter cake is reflux, extract, 3 hours under conditions of 20 times of water, supersonic frequency are 35kHz at solvent by second time, filters,
Obtain secondary filtrate, abandon secondary filter cake；First-time filtrate and secondary filtrate are merged, obtains amalgamation liquid；60-95% ethanol, mistake is added in above-mentioned amalgamation liquid
Filter, obtains merging filter cake, abandons merging filtrate；By above-mentioned merging filtration cakes torrefaction, obtain Chinese medicine composition pressed powder.
Embodiment 7
Weigh according to the Radix Astragali 2 weight portion, Fructus Ligustri Lucidi 1 weight portion；By broken for each medicated powder for coarse granule, coarse granule mixes, and obtains coarse granule mixture；
Be for the first time reflux, extract, 4 hours under conditions of 25 times of water, supersonic frequency are 40kHz by coarse granule mixture at solvent, filter, once filter
Liquid and a filter cake；Filter cake is reflux, extract, 4 hours under conditions of 25 times of water, supersonic frequency are 40kHz at solvent by second time, filters,
Obtain secondary filtrate, abandon secondary filter cake；First-time filtrate and secondary filtrate are merged, obtains amalgamation liquid；60-95% ethanol, mistake is added in above-mentioned amalgamation liquid
Filter, obtains merging filter cake, abandons merging filtrate；By above-mentioned merging filtration cakes torrefaction, obtain Chinese medicine composition pressed powder.
Embodiment 8
Weigh according to the Radix Astragali 2 weight portion, Fructus Ligustri Lucidi 1 weight portion；By broken for each medicated powder for coarse granule, coarse granule mixes, and obtains coarse granule mixture；
Be for the first time reflux, extract, 5 hours under conditions of 30 times of water, supersonic frequency are 45kHz by coarse granule mixture at solvent, filter, once filter
Liquid and a filter cake；Filter cake is reflux, extract, 5 hours under conditions of 30 times of water, supersonic frequency are 45kHz at solvent by second time, filters,
Obtain secondary filtrate, abandon secondary filter cake；First-time filtrate and secondary filtrate are merged, obtains amalgamation liquid；60-95% ethanol, mistake is added in above-mentioned amalgamation liquid
Filter, obtains merging filter cake, abandons merging filtrate；By above-mentioned merging filtration cakes torrefaction, obtain Chinese medicine composition pressed powder.
Embodiment 9
Weigh according to the Radix Astragali 2 weight portion, Fructus Ligustri Lucidi 1 weight portion；By broken for each medicated powder for coarse granule, coarse granule mixes, and obtains coarse granule mixture；
Be for the first time reflux, extract, 6 hours under conditions of 35 times of water, supersonic frequency are 50kHz by coarse granule mixture at solvent, filter, once filter
Liquid and a filter cake；Filter cake is reflux, extract, 6 hours under conditions of 35 times of water, supersonic frequency are 50kHz at solvent by second time, filters,
Obtain secondary filtrate, abandon secondary filter cake；First-time filtrate and secondary filtrate are merged, obtains amalgamation liquid；60-95% ethanol, mistake is added in above-mentioned amalgamation liquid
Filter, obtains merging filter cake, abandons merging filtrate；By above-mentioned merging filtration cakes torrefaction, obtain Chinese medicine composition pressed powder.
Embodiment 10
The assay of astragaloside:
Chromatographic condition and system suitability are with octadecylsilane chemically bonded silica as filler；With acetonitrile-water (32: 68) for flowing phase；Steam
Light Scattering Detector detects.Number of theoretical plate is calculated by astragaloside peak should be not less than 4000.
It is appropriate that the preparation of reference substance solution takes astragaloside reference substance, accurately weighed, adds methanol and makes every 1ml solution containing 0.5mg, to obtain final product.
In the pressed powder preparing Example 1-9 of need testing solution, powder about 4g, accurately weighed, puts in apparatus,Soxhlet's, adds methanol 40ml,
Overnight, then it is appropriate to add methanol, is heated to reflux 4 hours for merceration, and extracting solution recycling design is also concentrated to dryness, and residue adds water 10ml, and slight fever makes dissolving,
Extract 4 times with the shaking of water saturated n-butyl alcohol, each 40ml, merge n-butyl alcohol liquid, fully wash with ammonia solution 2 times, each 40ml, abandon
Going ammoniacal liquor, n-butyl alcohol liquid to be evaporated, the residue 5ml that adds water makes dissolving, lets cool, and by D101 type macroporous adsorptive resins, (internal diameter is 1.5cm, post
A height of 12cm), with water 50ml eluting, discard water liquid, then with 40% ethanol 30ml eluting, discard eluent, continue with 70% ethanol 80ml
Eluting, collects eluent, is evaporated, and residue adds methanol and dissolves, and is transferred in 5ml measuring bottle, adds methanol to scale, shakes up, to obtain final product.
Algoscopy precision respectively draws reference substance solution 10 μ l, 20 μ l, need testing solution 20 μ l, injects chromatograph of liquid, measures, uses
External standard two-point method logarithmic equation calculates, and to obtain final product, and concrete data are shown in Table 2.
Embodiment 11
The assay of calycosin glucoside:
Chromatographic condition and system suitability are with octadecylsilane chemically bonded silica as filler；With acetonitrile as mobile phase A, molten with 0.2% formic acid
Liquid is Mobile phase B, carries out gradient elution by regulation in table 1；Detection wavelength is 260nm.Number of theoretical plate is pressed calycosin glucoside peak and is calculated
It is not less than 3000.
Table 1
| Time (minute) | Mobile phase A (%) | Mobile phase B (%) |
| 0～20 | 20→40 | 80→60 |
| 20～30 | 40 | 60 |
It is appropriate that the preparation of reference substance solution takes calycosin glucoside reference substance, accurately weighed, adds methanol and makes every 1ml containing 50 μ g's
Solution, to obtain final product.
Pressed powder (crossing No. four sieves) the about 1g preparing Example 1-9 of need testing solution, accurately weighed, to put in round-bottomed flask, precision adds
Enter methanol 50ml, weighed weight, be heated to reflux 4 hours, let cool, more weighed weight, supply the weight of less loss with methanol, shake up, filter,
Precision measures subsequent filtrate 25ml, and recycling design is to dry, and residue adds methanol and dissolves, and is transferred in 5ml measuring bottle, adds methanol to scale, shakes up, i.e.
?.
Algoscopy precision respectively draws reference substance solution and each 10 μ l of need testing solution, injects chromatograph of liquid, measures, to obtain final product, concrete data
It is shown in Table 2.
Embodiment 12
The assay of specnuezhenside:
Chromatographic condition and system suitability are with octadecylsilane chemically bonded silica as filler；With methanol-water (40: 60) for flowing phase；Inspection
Survey wavelength is 224nm.Number of theoretical plate is calculated by specnuezhenside peak should be not less than 4000.
It is appropriate that the preparation of reference substance solution takes specnuezhenside reference substance, accurately weighed, adds methanol and makes every 1ml solution containing 0.25mg, to obtain final product.
Need testing solution prepare Example 1-9 pressed powder (cross No. three sieves) about 0.5g, accurately weighed, put in tool plug conical flask, precision
Add Diluted Alcohol 50ml, weighed weight, be heated to reflux 1 hour, let cool, more weighed weight, supply the weight of less loss with Diluted Alcohol, shake up,
Filter, take subsequent filtrate, to obtain final product.
Algoscopy precision respectively draws reference substance solution 5 μ l and need testing solution 10 μ l, injects chromatograph of liquid, measures, to obtain final product, specifically
Data are shown in Table 2.
The assay result of the astragaloside of table 2 embodiment 1-9, calycosin glucoside and specnuezhenside
In sum, embodiment 1-9 possesses significance through t inspection.The astragaloside of embodiment 2, calycosin glucoside, specnuezhenside
Content less than embodiment 1, the astragaloside of embodiment 3-9, calycosin glucoside, specnuezhenside content higher than embodiment 1, table
Bright ultrasonic extracting method of the present invention can improve astragaloside in Chinese medicine composition, calycosin glucoside, the content of specnuezhenside, contracting
Short production cycle, reduces production cost, improves production efficiency.
Obviously, the above embodiment of the present invention is only for clearly demonstrating example of the present invention, and is not the limit to embodiment of the present invention
Fixed.For the technical staff in described field, can also make other changes in different forms on the basis of the above description.Here without
Need also to give exhaustive to all of embodiment.And these spirit belonging to the present invention are amplified out obvious change or variation still in
Among protection scope of the present invention.
Claims (6)
1. a ultrasonic extracting method for Chinese medicine composition, it comprises the steps: to weigh according to the Radix Astragali 2 weight portion, Fructus Ligustri Lucidi 1 weight portion；
It is characterized in that, by broken for each medicated powder for coarse granule, coarse granule mixes, and obtains coarse granule mixture；For the first time by coarse granule mixture solvent be 5～
35 times of water, supersonic frequency are reflux, extract, 0.5～6 hours under conditions of 20～50kHz, filter, obtain first-time filtrate and a filter cake；For the second time
Being 5～35 times of water by filter cake at solvent, supersonic frequency is reflux, extract, 0.5～6 hours under conditions of 20～50kHz, filters, obtains secondary
Filtrate, abandons secondary filter cake；First-time filtrate and secondary filtrate are merged, obtains amalgamation liquid；In above-mentioned amalgamation liquid, add 60-95% ethanol, filter,
Merge filter cake, abandon merging filtrate；By above-mentioned merging filtration cakes torrefaction, obtain Chinese medicine composition pressed powder.
2. the ultrasonic extracting method of Chinese medicine composition described above, it comprises the steps: to claim according to the Radix Astragali 2 weight portion, Fructus Ligustri Lucidi 1 weight portion
Take；It is characterized in that, by broken for each medicated powder for coarse granule, coarse granule mixes, and obtains coarse granule mixture；By coarse granule mixture at solvent it is for the first time
10 times of water, supersonic frequency are reflux, extract, 1 hour under conditions of 25kHz, filter, obtain first-time filtrate and a filter cake；Second time will once be filtered
Cake is 10 times of water at solvent, and supersonic frequency is reflux, extract, 1 hour under conditions of 25kHz, filters, obtains secondary filtrate, abandon secondary filter cake；Will
First-time filtrate and secondary filtrate merge, and obtain amalgamation liquid；In above-mentioned amalgamation liquid, add 60-95% ethanol, filter, obtain merging filter cake, abandon merging filtrate；
By above-mentioned merging filtration cakes torrefaction, obtain Chinese medicine composition pressed powder.
3. the ultrasonic extracting method of Chinese medicine composition described above, it comprises the steps: to claim according to the Radix Astragali 2 weight portion, Fructus Ligustri Lucidi 1 weight portion
Take；It is characterized in that, by broken for each medicated powder for coarse granule, coarse granule mixes, and obtains coarse granule mixture；By coarse granule mixture at solvent it is for the first time
15 times of water, supersonic frequency are reflux, extract, 2 hours under conditions of 30kHz, filter, obtain first-time filtrate and a filter cake；Second time will once be filtered
Cake is 15 times of water at solvent, and supersonic frequency is reflux, extract, 2 hours under conditions of 30kHz, filters, obtains secondary filtrate, abandon secondary filter cake；Will
First-time filtrate and secondary filtrate merge, and obtain amalgamation liquid；In above-mentioned amalgamation liquid, add 60-95% ethanol, filter, obtain merging filter cake, abandon merging filtrate；
By above-mentioned merging filtration cakes torrefaction, obtain Chinese medicine composition pressed powder.
4. the ultrasonic extracting method of Chinese medicine composition described above, it comprises the steps: to claim according to the Radix Astragali 2 weight portion, Fructus Ligustri Lucidi 1 weight portion
Take；It is characterized in that, by broken for each medicated powder for coarse granule, coarse granule mixes, and obtains coarse granule mixture；By coarse granule mixture at solvent it is for the first time
20 times of water, supersonic frequency are reflux, extract, 3 hours under conditions of 35kHz, filter, obtain first-time filtrate and a filter cake；Second time will once be filtered
Cake is 20 times of water at solvent, and supersonic frequency is reflux, extract, 3 hours under conditions of 35kHz, filters, obtains secondary filtrate, abandon secondary filter cake；Will
First-time filtrate and secondary filtrate merge, and obtain amalgamation liquid；In above-mentioned amalgamation liquid, add 60-95% ethanol, filter, obtain merging filter cake, abandon merging filtrate；
By above-mentioned merging filtration cakes torrefaction, obtain Chinese medicine composition pressed powder.
5. the ultrasonic extracting method of Chinese medicine composition described above, it comprises the steps: to claim according to the Radix Astragali 2 weight portion, Fructus Ligustri Lucidi 1 weight portion
Take；It is characterized in that, by broken for each medicated powder for coarse granule, coarse granule mixes, and obtains coarse granule mixture；By coarse granule mixture at solvent it is for the first time
25 times of water, supersonic frequency are reflux, extract, 4 hours under conditions of 40kHz, filter, obtain first-time filtrate and a filter cake；Second time will once be filtered
Cake is 25 times of water at solvent, and supersonic frequency is reflux, extract, 4 hours under conditions of 40kHz, filters, obtains secondary filtrate, abandon secondary filter cake；Will
First-time filtrate and secondary filtrate merge, and obtain amalgamation liquid；In above-mentioned amalgamation liquid, add 60-95% ethanol, filter, obtain merging filter cake, abandon merging filtrate；
By above-mentioned merging filtration cakes torrefaction, obtain Chinese medicine composition pressed powder.
6. the ultrasonic extracting method of Chinese medicine composition described above, it comprises the steps: to claim according to the Radix Astragali 2 weight portion, Fructus Ligustri Lucidi 1 weight portion
Take；It is characterized in that, by broken for each medicated powder for coarse granule, coarse granule mixes, and obtains coarse granule mixture；By coarse granule mixture at solvent it is for the first time
30 times of water, supersonic frequency are reflux, extract, 5 hours under conditions of 45kHz, filter, obtain first-time filtrate and a filter cake；Second time will once be filtered
Cake is 30 times of water at solvent, and supersonic frequency is reflux, extract, 5 hours under conditions of 45kHz, filters, obtains secondary filtrate, abandon secondary filter cake；Will
First-time filtrate and secondary filtrate merge, and obtain amalgamation liquid；In above-mentioned amalgamation liquid, add 60-95% ethanol, filter, obtain merging filter cake, abandon merging filtrate；
By above-mentioned merging filtration cakes torrefaction, obtain Chinese medicine composition pressed powder.
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| CN108982738A (en) * | 2018-10-24 | 2018-12-11 | 吉林省现代中药工程研究中心有限公司 | The intermediate characteristic spectrum of zhenqi fuzheng granules or the construction method and detection method of zhenqi fuzheng granules finger-print |
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| CN1621083A (en) * | 2003-11-28 | 2005-06-01 | 甘肃扶正药物科技股份有限公司 | Improvement of method for preparing healthy qi strengthening, astragalus root and glossy privet fruit containing medicine |
| CN1831015A (en) * | 2006-04-26 | 2006-09-13 | 贵州信邦远东药业有限公司 | Process for extracting fructus ligustri lucidi and Radixa stragali polysaccharide, the products and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108982738A (en) * | 2018-10-24 | 2018-12-11 | 吉林省现代中药工程研究中心有限公司 | The intermediate characteristic spectrum of zhenqi fuzheng granules or the construction method and detection method of zhenqi fuzheng granules finger-print |
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Application publication date: 20161207 |