CN102988526B - Method for preparing Bailing tablets and application - Google Patents
Method for preparing Bailing tablets and application Download PDFInfo
- Publication number
- CN102988526B CN102988526B CN 201210377391 CN201210377391A CN102988526B CN 102988526 B CN102988526 B CN 102988526B CN 201210377391 CN201210377391 CN 201210377391 CN 201210377391 A CN201210377391 A CN 201210377391A CN 102988526 B CN102988526 B CN 102988526B
- Authority
- CN
- China
- Prior art keywords
- bailing
- pian
- extraction
- preparation
- ethanol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a method for preparing Bailing tablets. The Bailing tablets are prepared from 194g of Chinese angelica, 42g of pseudo-ginseng, 83g of safflower, 167g of peony bark, 83g of peach kernel, 167g of divaricate saposhnikovia root, 167g of atractylodes, 42g of angelica root, 417g of purslane, 83g of red peony root and 194g of astragalus root serving as raw medicaments by adopting supercritical extraction and microwave extraction, so that the ferulic acid content is greatly improved. The invention also provides application of the Bailing tablets in preparation of a medicament for inhibiting propagation of human histiocytic lymphoma U937 cells.
Description
Technical field
The present invention relates to the Chinese medicine preparation technical field, be specifically related to a kind of preparation method and application of BAILING PIAN.
Background technology
BAILING PIAN is recorded in Ministry of Public Health standard WS3-B-0914-91, by Radix Angelicae Sinensis 194g, Radix Notoginseng 42g, Flos Carthami 83g, Cortex Moutan 167g, Semen Persicae 83g, Radix Saposhnikoviae 167g, Rhizoma Atractylodis 167g, Radix Angelicae Dahuricae 42g, Herba Portulacae 417g, Radix Paeoniae Rubra 83g, Radix Astragali 194g, as crude drug, made, the energy blood circulation promoting and blood stasis dispelling, increase photosensitization.For vitiligo.
In prior art, not yet there is BAILING PIAN to adopt the report of supercritical and microwave technology extracting aspect preparation, and adopt the method that powder and decocting boil of beating, technique is coarse, backward, and impurity is many, causes patient's consumption excessive, be inconvenient to take, had a strong impact on this product and applied clinically.
Summary of the invention
Goal of the invention: in order to address the above problem, the object of the present invention is to provide a kind of preparation method of BAILING PIAN.
Another object of the present invention is to provide a kind of BAILING PIAN to suppress the application in the lymphoma U937 of human tissue cell cell proliferation medicine in preparation.
Technical scheme: the objective of the invention is to realize by following scheme:
A kind of preparation method of BAILING PIAN, by Radix Angelicae Sinensis 194g, Radix Notoginseng 42g, Flos Carthami 83g, Cortex Moutan 167g, Semen Persicae 83g, Radix Saposhnikoviae 167g, Rhizoma Atractylodis 167g, Radix Angelicae Dahuricae 42g, Herba Portulacae 417g, Radix Paeoniae Rubra 83g, Radix Astragali 194g, as crude drug, made, described method is comprised of the following step: get Radix Angelicae Sinensis, Flos Carthami, Semen Persicae, Rhizoma Atractylodis, join in CO2 supercritical extraction device, ethanol is as entrainer, the percent by volume that entrainer accounts for total extractant is 4-6%, extracting pressure 15-30MPa, temperature 30-50 ℃, CO
2flow 1-3ml/g crude drug min, extraction time 150-180min, obtain supercritical extract, standby; Get all the other Chinese medicines, pulverize, add 70% ethanol of 2L, drop in microwave extracting apparatus and carry out microwave extracting, extraction power 400-600W, extract 2 times, each 4-8 minute, combining extraction liquid, concentrated, be added on the D101 macroporous adsorptive resins, 50% ethanol elution, collect 5 times of amount column volume eluents, decompression recycling ethanol, concentrated and dry, obtain the microwave extraction thing, standby; Above-mentioned supercritical extract and microwave extraction thing are mixed, add starch, 70% ethanol granule processed, drying, tabletting, make 1000, every heavy 0.5g.
The preparation method of above-mentioned a kind of BAILING PIAN, described CO
2the percent by volume that the supercritical extraction entrainer accounts for total extractant is 5%.
The preparation method of above-mentioned a kind of BAILING PIAN, described microwave extracting power 500W extracts 6 minutes at every turn.
The preparation method of above-mentioned a kind of BAILING PIAN, described CO
2the extracting pressure 20MPa of supercritical extraction, 40 ℃ of temperature, CO
2flow 2ml/g crude drug min, extraction time 160min.
Above-mentioned BAILING PIAN suppresses the application in the lymphoma U937 of human tissue cell cell proliferation medicine in preparation.
In prior art, every 0.5g of BAILING PIAN, each 4,3 times on the one, the every 0.5g of BAILING PIAN that adopts the present invention to be prepared into only needs 2 at every turn, within 1st, takes 3 times, has greatly reduced dose having under the condition of more active component.This conclusion can be by following evidence.
The comparison of ferulaic acid content in BAILING PIAN prepared by test one, distinct methods
1, instrument and reagent BAILING PIAN of the present invention: press embodiment 3 method preparations, use the 1639g crude drug, through extraction, make 1000, every heavy 0.5g.Former BAILING PIAN, by the preparation of ministry standard method, used the 1639g crude drug, through extraction, makes 1000, every heavy 0.5g.Agilent 1200 high performance liquid chromatographs; METTLER AE240 electronic analytical balance; Ferulic acid reference substance (Nat'l Pharmaceutical & Biological Products Control Institute).
2, method
Chromatographic condition and system suitability: with octadecylsilane chemically bonded silica, be filler; Methanol-water-phosphoric acid (40:60:0.2) is mobile phase; The detection wavelength is 280nm.Number of theoretical plate is pressed the ferulic acid peak and is calculated, and should be not less than 3000.
The preparation of reference substance solution: precision takes at 60 ℃ of drying under reduced pressure ferulic acid reference substance of 4 hours appropriate, adds methanol and makes the solution of every 1ml containing 18 μ g, obtains.
The preparation of need testing solution: get BAILING PIAN of the present invention and former BAILING PIAN, porphyrize, mix, and gets 1g, accurately weighed, and precision adds 70% ethanol 20ml, close plug, and supersound process 10 minutes, centrifugal, get supernatant, obtain.
Algoscopy is accurate reference substance solution and each 20 μ l of need testing solution of drawing respectively, and the injection liquid chromatography, measure, and obtains.
3, result
Result shows, in BAILING PIAN of the present invention, the content of ferulic acid is the 1.53mg/ sheet; And in former BAILING PIAN, the content of ferulic acid is the 0.28mg/ sheet, in the situation that dose reduces, ferulaic acid content improves a lot.
Above-mentioned research shows, the BAILING PIAN that adopts the present invention to prepare, the BAILING PIAN that active constituent content is standby higher than the ministry standard legal system.
The specific embodiment
Form by the following examples, foregoing of the present invention is described in further detail again, but this should be interpreted as to the scope of the above-mentioned theme of the present invention only limits to following example, all technology realized based on foregoing of the present invention all belong to scope of the present invention.
Embodiment 1
Get Radix Angelicae Sinensis 194g, Radix Notoginseng 42g, Flos Carthami 83g, Cortex Moutan 167g, Semen Persicae 83g, Radix Saposhnikoviae 167g, Rhizoma Atractylodis 167g, Radix Angelicae Dahuricae 42g, Herba Portulacae 417g, Radix Paeoniae Rubra 83g, the Radix Astragali 194, by Radix Angelicae Sinensis, Flos Carthami, Semen Persicae, Rhizoma Atractylodis, join in CO2 supercritical extraction device, ethanol is as entrainer, the percent by volume that entrainer accounts for total extractant is 4%, extracting pressure 15MPa, 30 ℃ of temperature, CO
2flow 1ml/g crude drug min, extraction time 150min, obtain supercritical extract, standby; Get all the other Chinese medicines, pulverize, add 70% ethanol of 2L, drop in microwave extracting apparatus and carry out microwave extracting, extraction power 400W, extract 2 times, each 4 minutes, combining extraction liquid, concentrated, be added on the D101 macroporous adsorptive resins, 50% ethanol elution, collect 5 times of amount column volume eluents, decompression recycling ethanol, concentrated and dry, obtain the microwave extraction thing, standby; Above-mentioned supercritical extract and microwave extraction thing are mixed, add starch, 70% ethanol granule processed, drying, tabletting, make 1000, every heavy 0.5g.
After testing, in finished product, the content of ferulic acid is the 1.64mg/ sheet.
Embodiment 2
Get Radix Angelicae Sinensis 194g, Radix Notoginseng 42g, Flos Carthami 83g, Cortex Moutan 167g, Semen Persicae 83g, Radix Saposhnikoviae 167g, Rhizoma Atractylodis 167g, Radix Angelicae Dahuricae 42g, Herba Portulacae 417g, Radix Paeoniae Rubra 83g, the Radix Astragali 194, by Radix Angelicae Sinensis, Flos Carthami, Semen Persicae, Rhizoma Atractylodis, join CO
2in the supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 6%, extracting pressure 30MPa, temperature 50 C, CO
2flow 3ml/g crude drug min, extraction time 180min, obtain supercritical extract, standby; Get all the other Chinese medicines, pulverize, add 70% ethanol of 2L, drop in microwave extracting apparatus and carry out microwave extracting, extraction power 600W, extract 2 times, each 8 minutes, combining extraction liquid, concentrated, be added on the D101 macroporous adsorptive resins, 50% ethanol elution, collect 5 times of amount column volume eluents, decompression recycling ethanol, concentrated and dry, obtain the microwave extraction thing, standby; Above-mentioned supercritical extract and microwave extraction thing are mixed, add starch, 70% ethanol granule processed, drying, tabletting, make 1000, every heavy 0.5g.
After testing, in finished product, the content of ferulic acid is the 1.61mg/ sheet.
Embodiment 3
Get Radix Angelicae Sinensis 194g, Radix Notoginseng 42g, Flos Carthami 83g, Cortex Moutan 167g, Semen Persicae 83g, Radix Saposhnikoviae 167g, Rhizoma Atractylodis 167g, Radix Angelicae Dahuricae 42g, Herba Portulacae 417g, Radix Paeoniae Rubra 83g, the Radix Astragali 194, by Radix Angelicae Sinensis, Flos Carthami, Semen Persicae, Rhizoma Atractylodis, join CO
2in the supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 5%, extracting pressure 20MPa, 40 ℃ of temperature, CO
2flow 2m1/g crude drug min, extraction time 160min, obtain supercritical extract, standby; Get all the other Chinese medicines, pulverize, add 70% ethanol of 2L, drop in microwave extracting apparatus and carry out microwave extracting, extraction power 500W, extract 2 times, each 6 minutes, combining extraction liquid, concentrated, be added on the D101 macroporous adsorptive resins, 50% ethanol elution, collect 5 times of amount column volume eluents, decompression recycling ethanol, concentrated and dry, obtain the microwave extraction thing, standby; Above-mentioned supercritical extract and microwave extraction thing are mixed, add starch, 70% ethanol granule processed, drying, tabletting, make 1000, every heavy 0.5g.
After testing, in finished product, the content of ferulic acid is the 1.53mg/ sheet.
Embodiment 4: BAILING PIAN suppresses the experimentation data of U937 cell proliferation
1 experiment material
1.1 experiment cell strain
The lymphoma U937 of human tissue cell cell, Nanjing Zhengkuan Pharmaceutical Technology Co., Ltd.'s laboratory cell bank, DMEM+10% FBS cellar culture.
1.2 Experimental agents
Drugs: BAILING PIAN of the present invention: press embodiment 3 method preparations.
The medicinal liquid liquid storage: take the 100mg BAILING PIAN, be dissolved in the 5ml dehydrated alcohol, 0.2 μ m filter filters, 500 μ l doff pipe packing, and-20 ℃ of storages, 0.2 μ m filter filters the use of dehydrated alcohol in order to matched group simultaneously.
1.3 experiment reagent
The Cat.No.12100-061 Lot.No.758137 of DMEM(GIBCO company); Hyclone (Hangzhoupro, sky, Zhejiang bio tech ltd Lot.No.100419); NaHCO3(Shanghai hundred million chemical reagent company limited Cat.No.11810-033 Lot.No.1088387 of a specified duration); Trypsin(AMRESCO company lot number: 2010/04); EDTA(AMRESCO company lot number: 2009/10); Penicillin G Sodium Salt(AMRESCO company lot number: 2010242); Streptomycin Sulfate(AMRESCO company lot number: 2010382); Dehydrated alcohol (Nanjing Chemistry Reagent Co., Ltd.'s lot number: 080310182); MTT (Biosharp lot number: 0793); The autogamy of PBS(laboratory);
1.4 experiment equipment
Lycra inverted microscope (German Leica model: DM1L); Visible-ultraviolet light microwell plate detector (U.S. MD company model: SPECTRA MAX 190); CO2 incubator (FORMA model: 3111); (safe and sound company of Su Jing group manufactures model to super-clean bench: SW-CJ-ZFD); Pure water instrument (U.S. Spring company model: S/N 020579); Accurate pipettor (French Gilson Inc model: P2); Electronic balance (German Sai Duolisi company limited model: BT323S); Full-automatic high-pressure autoclave (Japanese SANYO company model: MLS-3020); Table electrothermal air dry oven (Shanghai accurate experimental facilities company model: DHG9123A); Refrigerator (Siemens Company's model: KG18V21TI); Liquid nitrogen container (CBS model: 2001); Low speed centrifuge (Anting Scientific Instrument Factory, Shanghai's model: KA-1000); 0.2 μ m filter (MILLIPORE model: SLGP033RB); 10cm culture dish (NEST company), 96 well culture plates (NEST company); Cell counting count board; Centrifuge tube, pipet, Tips are some.
2 experimental techniques
1) the U937 cell carries out cellar culture (10cm culture dish) with DMEM+10% FBS in 37 ℃, 5% CO2, when Growth of Cells during to logarithmic (log) phase, collecting cell, discard culture fluid, PBS fine laundering 3 times, add 3ml 0.25% trypsin-0.04% EDTA, after 37 ℃ of digestion 2min, add wherein 5ml complete medium neutralization reaction, after the piping and druming cell, it is proceeded in centrifuge tube, the centrifugal 5min of 1000rpm, adjust 3 * 104/ml of concentration of cell suspension.
2) the cell kind is entered in 96 well culture plates, every hole adds cell suspension 180 μ l, and culture plate is put into cell culture incubator (37 ℃, 5% CO2) cellar culture.
3) according to the Growth of Cells situation, generally grow to 50%-70%, add BAILING PIAN solution, continue to cultivate 24h.
4) add 20 μ l MTT solution (5mg/ml, i.e. 0.5%MTT) after 24h, continue to cultivate 4h.
5) after 4h, the buckle method is removed supernatant, with absorbent paper, pats dry gently, and every hole adds 200 μ l dimethyl sulfoxide, puts low-speed oscillation 10min on shaking table, and crystal is fully dissolved.Measure the light absorption value in each hole at enzyme-linked immunosorbent assay instrument 490nm place.6) background (do not add cell, only add culture fluid) is set simultaneously, control wells (the medicine dissolution medium of cell, same concentrations, culture fluid, MTT, dimethyl sulfoxide), set 6 multiple holes for every group.
7) with medicine, the suppression ratio to cell means result:
Cell increment suppression ratio (%)=(control wells OD value-dosing holes OD value)/control wells OD value * 100%.Experiment repeats 3 times.
3 statistical dispositions
Adopt correlation analysis and Student t check in Microsoft Excel 2003 softwares, data mean with mean ± S.D..
4 experimental results
Statistical result showed after the mtt assay experiment, with matched group, compare, when dosage reaches 5mg/ml, to U937 cell inhibitory effect variant (P<0.05), dosage this difference when 10mg/ml has significance (P<0.01), and utmost point significant difference (P<0.001) is arranged when dosage reaches 15-20mg/ml.
Table 1 BAILING PIAN is on U937 cell inhibitory effect impact research
Annotate: compare * P<0.01 with matched group; * P<0.001
5 experiment conclusion
BAILING PIAN can suppress the U937 cell proliferation, reduces the Growth of Cells number of U937 cell, and this effect is dose dependent.
Claims (4)
1. a BAILING PIAN suppresses the application in the lymphoma U937 of human tissue cell cell proliferation medicine in preparation, it is characterized in that BAILING PIAN made as crude drug by Radix Angelicae Sinensis 194g, Radix Notoginseng 42g, Flos Carthami 83g, Cortex Moutan 167g, Semen Persicae 83g, Radix Saposhnikoviae 167g, Rhizoma Atractylodis 167g, Radix Angelicae Dahuricae 42g, Herba Portulacae 417g, Radix Paeoniae Rubra 83g, Radix Astragali 194g, preparation method is comprised of the following step: get Radix Angelicae Sinensis, Flos Carthami, Semen Persicae, Rhizoma Atractylodis, join CO
2in the supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 4-6%, extracting pressure 15-30MPa, temperature 30-50 ℃, CO
2flow 1-3m1/g crude drug min, extraction time 150-180min, obtain supercritical extract, standby; Get all the other Chinese medicines, pulverize, add 70% ethanol of 2L, drop in microwave extracting apparatus and carry out microwave extracting, extraction power 400-600W, extract 2 times, each 4-8 minute, combining extraction liquid, concentrated, be added on the D101 macroporous adsorptive resins, 50% ethanol elution, collect 5 times of amount column volume eluents, decompression recycling ethanol, concentrated and dry, obtain the microwave extraction thing, standby; Above-mentioned supercritical extract and microwave extraction thing are mixed, add starch, 70% ethanol granule processed, drying, tabletting, make 1000, every heavy 0.5g.
2. a kind of BAILING PIAN suppresses the application in the lymphoma U937 of human tissue cell cell proliferation medicine in preparation according to claim 1, it is characterized in that the percent by volume that CO2 supercritical extraction entrainer described in the preparation method of BAILING PIAN accounts for total extractant is 5%.
3. a kind of BAILING PIAN suppresses the application in the lymphoma U937 of human tissue cell cell proliferation medicine in preparation according to claim 1, it is characterized in that microwave extracting power 500W described in the preparation method of BAILING PIAN, extracts 6 minutes at every turn.
4. a kind of BAILING PIAN suppresses the application in the lymphoma U937 of human tissue cell cell proliferation medicine in preparation according to claim 1, it is characterized in that CO described in the preparation method of BAILING PIAN
2the extracting pressure 20MPa of supercritical extraction, 40 ℃ of temperature, CO
2flow 2ml/g crude drug min, extraction time 160min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210377391 CN102988526B (en) | 2012-10-08 | 2012-10-08 | Method for preparing Bailing tablets and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210377391 CN102988526B (en) | 2012-10-08 | 2012-10-08 | Method for preparing Bailing tablets and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102988526A CN102988526A (en) | 2013-03-27 |
| CN102988526B true CN102988526B (en) | 2013-12-25 |
Family
ID=47917953
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201210377391 Expired - Fee Related CN102988526B (en) | 2012-10-08 | 2012-10-08 | Method for preparing Bailing tablets and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102988526B (en) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103655839B (en) * | 2013-12-11 | 2015-07-15 | 周武元 | Preparation method of Yikangbuyuan tablet and application of Yikangbuyuan tablet in medicines for inhibiting mouse lung cancer cell LLC proliferation |
| CN103655838B (en) * | 2013-12-11 | 2015-06-24 | 邵文博 | Preparation method of Yikangbuyuan tablet and application of Yikangbuyuan tablet in medicines for inhibiting mouse lymphomata cell L5178Y proliferation |
| CN103690646B (en) * | 2013-12-11 | 2015-08-19 | 徐茂航 | Preparation method of Yikangbuyuan tablet and application of Yikangbuyuan tablet in medicine for inhibiting mouse lymphoma cell YAC-1 cell proliferation |
| CN103655842B (en) * | 2013-12-11 | 2015-06-03 | 冯向英 | Preparation method of Yikangbuyuan tablet and application of Yikangbuyuan tablet in medicines for inhibiting mouse lymphomata cell EL4. IL-2 proliferation |
| CN103655843B (en) * | 2013-12-11 | 2016-01-27 | 董广才 | A kind of Yikang complement sheet and its preparation method and application |
| CN103655841B (en) * | 2013-12-11 | 2015-05-20 | 刘伟伟 | Preparation method of Yikangbuyuan tablet and application of Yikangbuyuan tablet in medicines for inhibiting mouse lymphoid cancer cell P388D1 proliferation |
| CN103655840B (en) * | 2013-12-11 | 2015-07-15 | 周武元 | Preparation method of Yikangbuyuan tablet and application of Yikangbuyuan tablet in medicines for inhibiting mouse leukaemia cell L1210 proliferation |
| CN104383282A (en) * | 2014-11-13 | 2015-03-04 | 山东中大药业有限公司 | Preparation method of nine-ingredient false Chinese swertia herb tablets and application of nine-ingredient false Chinese swertia herb tablets to preparation of medicines for inhibiting proliferation of hepatoma cell Hepa1-6 |
| CN104398818A (en) * | 2014-11-13 | 2015-03-11 | 山东中大药业有限公司 | Preparation method of nine flavor swertia bimaculata tablet and application of nine flavor swertia bimaculata tablet in preparation of drugs for inhibition of cell proliferation of myeloma cell SP2/0 |
| CN104398815A (en) * | 2014-11-13 | 2015-03-11 | 山东中大药业有限公司 | Preparation method of nine flavor swertia bimaculata tablet and application of nine flavor swertia bimaculata tablet in preparation of drugs for inhibition of cell proliferation of melanoma cell B16 |
| CN104306726A (en) * | 2014-11-13 | 2015-01-28 | 山东中大药业有限公司 | Preparation method of nine-herb false Chinese swertia herb tablets and application of nine-herb false Chinese swertia herb tablets in preparation of medicament for inhibiting cell proliferation of myeloma cell FO |
| CN109329940A (en) * | 2018-12-14 | 2019-02-15 | 洛阳采方医药科技有限公司 | A kind of food grade antioxidants and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102397451A (en) * | 2011-11-26 | 2012-04-04 | 苏州派腾生物医药科技有限公司 | Preparation method of cholagogic tablet |
-
2012
- 2012-10-08 CN CN 201210377391 patent/CN102988526B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102397451A (en) * | 2011-11-26 | 2012-04-04 | 苏州派腾生物医药科技有限公司 | Preparation method of cholagogic tablet |
Non-Patent Citations (3)
| Title |
|---|
| 中华人民共和国***药典委员会.百灵片.《***药品标准 中药成方制剂分册（第五册）》.1992,45. * |
| 梁汉明等.苍术超临界CO2萃取物的质量标准研究.《广东药学院学报》.2002,第18卷(第04期),267-270. |
| 苍术超临界CO2萃取物的质量标准研究;梁汉明等;《广东药学院学报》;20021231;第18卷(第04期);267-270 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102988526A (en) | 2013-03-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102988526B (en) | Method for preparing Bailing tablets and application | |
| CN102988723A (en) | Preparation method and application of yin tonifying and calming tablet | |
| CN102988667B (en) | Preparation method and application of tablet for regulating menstruation and activating blood | |
| CN102973667A (en) | Preparation method and application of cold-fever tablet | |
| CN102988577B (en) | Preparation method and application of brain-soothing hypertension pill | |
| CN102988665B (en) | Method for preparing gynecological menstruation regulating tablets and application | |
| CN102988612B (en) | Preparation method and application of tablet for clearing away heat | |
| CN102988545B (en) | Preparation method and application of Yulian tablet | |
| CN102988502B (en) | Preparation method and application of Desheng tablet | |
| CN103028005B (en) | Preparation method of anti-lumbago tablet and application thereof | |
| CN102988489B (en) | Preparation method and application of nose comforting tablet | |
| CN102988550B (en) | Preparation method and application of qinlian tablet | |
| CN102988479B (en) | Preparation method and application of anti-dazzling tablet | |
| CN102988480A (en) | Method for preparing bile and patchouli rhinitis tablets and application | |
| CN102988500B (en) | Method for preparing compound Dantong tablets and application | |
| CN102836381A (en) | Preparation method and application of middle calming tablet | |
| CN102988614A (en) | Preparation method and application of antiphlogosis tablet | |
| CN102988611B (en) | Preparation method and application of antihypertension tablet | |
| CN102988509B (en) | Preparation method and application of bone-knitting tablet | |
| CN102973749A (en) | Preparation method and applications of divaricate saposhnikovia-angelica archang lica rhinitis tablets | |
| CN102988501B (en) | Preparation method and application of Libiling tablet | |
| CN102988881B (en) | Preparation method and application of pulse unblocking tablet | |
| CN102988522B (en) | Preparation method and application of naodesheng tablets | |
| CN102988748B (en) | Preparation method and application of yin tonifying and blood sugar reducing tablet | |
| CN102988749B (en) | Preparation method and application of body-building tablet |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| ASS | Succession or assignment of patent right |
Owner name: LI SHAOBO Effective date: 20131122 Owner name: LI KUAN Free format text: FORMER OWNER: BIAN YUPING Effective date: 20131122 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| C53 | Correction of patent for invention or patent application | ||
| CB03 | Change of inventor or designer information |
Inventor after: Li Kuan Inventor after: Li Shaobo Inventor before: The inventor has waived the right to be mentioned |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 211224 NANJING, JIANGSU PROVINCE TO: 200433 YANGPU, SHANGHAI Free format text: CORRECT: INVENTOR; FROM: REQUEST NOT TO RELEASE THE NAME TO: LI KUAN LI SHAOBO |
|
| TA01 | Transfer of patent application right |
Effective date of registration: 20131122 Address after: 200433 Handan Road, Shanghai, No. 220, No. Applicant after: Li Kuan Applicant after: Li Shaobo Address before: Lishui County of Nanjing City, Jiangsu province 211224 Jing Qiao Zhen Chen Biancun Applicant before: Bian Yuping |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131225 Termination date: 20141008 |
|
| EXPY | Termination of patent right or utility model |