CN103494885A - Preparation method and application of orifice-freeing rhinitis tablets - Google Patents
Preparation method and application of orifice-freeing rhinitis tablets Download PDFInfo
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- CN103494885A CN103494885A CN201310473825.0A CN201310473825A CN103494885A CN 103494885 A CN103494885 A CN 103494885A CN 201310473825 A CN201310473825 A CN 201310473825A CN 103494885 A CN103494885 A CN 103494885A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 28
- 206010039083 rhinitis Diseases 0.000 title abstract 4
- 239000003814 drug Substances 0.000 claims abstract description 27
- 238000000194 supercritical-fluid extraction Methods 0.000 claims abstract description 16
- 241000628997 Flos Species 0.000 claims abstract description 13
- 230000004663 cell proliferation Effects 0.000 claims abstract description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 31
- 229940079593 drug Drugs 0.000 claims description 16
- 239000009670 cang er zi wan Substances 0.000 claims description 13
- 239000009636 Huang Qi Substances 0.000 claims description 12
- 238000000605 extraction Methods 0.000 claims description 12
- 206010020880 Hypertrophy Diseases 0.000 claims description 8
- 206010028980 Neoplasm Diseases 0.000 claims description 8
- 201000011510 cancer Diseases 0.000 claims description 8
- 239000008187 granular material Substances 0.000 claims description 8
- 229920002472 Starch Polymers 0.000 claims description 7
- 238000001035 drying Methods 0.000 claims description 7
- 239000000284 extract Substances 0.000 claims description 7
- 235000019698 starch Nutrition 0.000 claims description 7
- 239000008107 starch Substances 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 6
- 230000002401 inhibitory effect Effects 0.000 abstract description 3
- 239000002994 raw material Substances 0.000 abstract 2
- 241000045403 Astragalus propinquus Species 0.000 abstract 1
- 241000092665 Atractylodes macrocephala Species 0.000 abstract 1
- 201000009030 Carcinoma Diseases 0.000 abstract 1
- 235000006679 Mentha X verticillata Nutrition 0.000 abstract 1
- 235000002899 Mentha suaveolens Nutrition 0.000 abstract 1
- 235000001636 Mentha x rotundifolia Nutrition 0.000 abstract 1
- 241001180873 Saposhnikovia divaricata Species 0.000 abstract 1
- 241001506766 Xanthium Species 0.000 abstract 1
- 235000006533 astragalus Nutrition 0.000 abstract 1
- 235000013399 edible fruits Nutrition 0.000 abstract 1
- 210000003630 histaminocyte Anatomy 0.000 abstract 1
- 229960004756 ethanol Drugs 0.000 description 10
- 238000002474 experimental method Methods 0.000 description 8
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 4
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 3
- 230000010261 cell growth Effects 0.000 description 3
- 239000012531 culture fluid Substances 0.000 description 3
- 229960000935 dehydrated alcohol Drugs 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 230000001629 suppression Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- WEEMDRWIKYCTQM-UHFFFAOYSA-N 2,6-dimethoxybenzenecarbothioamide Chemical compound COC1=CC=CC(OC)=C1C(N)=S WEEMDRWIKYCTQM-UHFFFAOYSA-N 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 229920002334 Spandex Polymers 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000010165 autogamy Effects 0.000 description 1
- FCPVYOBCFFNJFS-LQDWTQKMSA-M benzylpenicillin sodium Chemical compound [Na+].N([C@H]1[C@H]2SC([C@@H](N2C1=O)C([O-])=O)(C)C)C(=O)CC1=CC=CC=C1 FCPVYOBCFFNJFS-LQDWTQKMSA-M 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000010219 correlation analysis Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 230000006837 decompression Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000012738 dissolution medium Substances 0.000 description 1
- 231100000673 dose–response relationship Toxicity 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000004900 laundering Methods 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012567 medical material Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 239000004759 spandex Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 229960002385 streptomycin sulfate Drugs 0.000 description 1
- 238000009495 sugar coating Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 235000019640 taste Nutrition 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
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- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention provides a preparation method of orifice-freeing rhinitis tablets. The orifice-freeing rhinitis tablets are prepared by performing supercritical extraction on 120 g of fried cocklebur fruit, 90 g of saposhnikovia divaricata, 150 g of astragalus membranaceus, 90 g of radix angelicae, 90 g of flos magnoliae, 90 g of fried atractylodes macrocephala koidz, and 30 g of mint serving as raw materials, so that the content of each raw material is greatly improved and the dose is reduced. The invention also discloses application of the orifice-freeing rhinitis tablets in preparation of a medicament for inhibiting the cell proliferation of mouse mast cell carcinoma P815.
Description
Technical field
The present invention relates to the Chinese medicine preparation technical field, be specifically related to a kind of preparation method and application of TONGXIAO BIYAN PIAN.
Background technology
TONGXIAO BIYAN PIAN is recorded in pharmacopeia, prescription is Fructus Xanthii (stir-fry) 120g, Radix Saposhnikoviae 90g, Radix Astragali 150g, Radix Angelicae Dahuricae 90g, Flos Magnoliae 90g, the Rhizoma Atractylodis Macrocephalae (stir-fry) 90g, Herba Menthae 30g, above seven flavors, get the Radix Angelicae Dahuricae, Rhizoma Atractylodis Macrocephalae 50g is ground into fine powder, the five tastes such as the residue Rhizoma Atractylodis Macrocephalae and all the other Fructus Xanthii, decoct with water secondary, each 2 hours, collecting decoction, filter, it is 1.28～1.32(80 ℃ that filtrate decompression is concentrated into relative density) clear paste, with above-mentioned powder, mix, dry, pulverize, granulate, be pressed into 600, sugar coating, obtain.
In prior art, not yet there is TONGXIAO BIYAN PIAN adopting the report of supercritical technology aspect the extraction preparation, and the method that adopts decocting to boil, technique is coarse, backward, and impurity is many, causes patient's consumption excessive, is inconvenient to take, and has had a strong impact on this product and has applied clinically.
Summary of the invention
Goal of the invention: in order to address the above problem, the object of the present invention is to provide a kind of preparation method of TONGXIAO BIYAN PIAN.
Another object of the present invention is to provide a kind of TONGXIAO BIYAN PIAN to suppress the application in mouse hypertrophy cell cancer P815 cell proliferation medicine in preparation.
Technical scheme: the objective of the invention is to realize by following scheme:
A kind of preparation method of TONGXIAO BIYAN PIAN, by Fructus Xanthii (stir-fry) 120g, Radix Saposhnikoviae 90g, Radix Astragali 150g, Radix Angelicae Dahuricae 90g, Flos Magnoliae 90g, the Rhizoma Atractylodis Macrocephalae, being fried) 90g, Herba Menthae 30g make as crude drug, described method is comprised of the following step: get Fructus Xanthii (stir-fry) 120g, Radix Saposhnikoviae 90g, Radix Astragali 150g, Radix Angelicae Dahuricae 90g, Flos Magnoliae 90g, the Rhizoma Atractylodis Macrocephalae and fry) 90g, Herba Menthae 30g join in CO2 supercritical extraction device, ethanol is as entrainer, the percent by volume that entrainer accounts for total extractant is 4-6%, extracting pressure 15-30MPa, temperature 30-50 ℃, CO
2flow 1-3m1/g crude drug min, extraction time 150-180min, obtain supercritical extract, adds starch, 70% ethanol granule processed, drying, tabletting, make 300, every heavy 0.5g.
The preparation method of above-mentioned a kind of TONGXIAO BIYAN PIAN, described CO
2the percent by volume that the supercritical extraction entrainer accounts for total extractant is 5%.
The preparation method of above-mentioned a kind of TONGXIAO BIYAN PIAN, described CO
2the extracting pressure 20MPa of supercritical extraction, 40 ℃ of temperature, CO
2flow 2ml/g crude drug min, extraction time 160min.
Above-mentioned a kind of TONGXIAO BIYAN PIAN suppresses the application in mouse hypertrophy cell cancer P815 cell proliferation medicine in preparation, TONGXIAO BIYAN PIAN is made as crude drug by Fructus Xanthii (parched) 120g, Radix Saposhnikoviae 90g, Radix Astragali 150g, Radix Angelicae Dahuricae 90g, Flos Magnoliae 90g, Rhizoma Atractylodis Macrocephalae (parched) 90g, Herba Menthae 30g, preparation method is comprised of the following step: get Fructus Xanthii (parched) 120g, Radix Saposhnikoviae 90g, Radix Astragali 150g, Radix Angelicae Dahuricae 90g, Flos Magnoliae 90g, Rhizoma Atractylodis Macrocephalae (parched) 90g, Herba Menthae 30g, join CO
2in the supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 4-6%, extracting pressure 15-30MPa, temperature 30-50 ℃, CO
2flow 1-3m1/g crude drug min, extraction time 150-180min, obtain supercritical extract, adds starch, 70% ethanol granule processed, drying, tabletting, make 300, every heavy 0.5g.
Above-mentioned a kind of TONGXIAO BIYAN PIAN suppresses the application in mouse hypertrophy cell cancer P815 cell proliferation medicine in preparation, and the percent by volume that the entrainer of CO2 supercritical extraction described in the preparation method of TONGXIAO BIYAN PIAN accounts for total extractant is 5%.
Above-mentioned a kind of TONGXIAO BIYAN PIAN suppresses the application in mouse hypertrophy cell cancer P815 cell proliferation medicine in preparation, the extracting pressure 20MPa of CO2 supercritical extraction described in the preparation method of TONGXIAO BIYAN PIAN, 40 ℃ of temperature, CO2 flow 2ml/g crude drug min, extraction time 160min.
In prior art, every 0.5g of TONGXIAO BIYAN PIAN, each 5,3 times on the one, the every 0.5g of TONGXIAO BIYAN PIAN that adopts the present invention to be prepared into, but the medical material amount contained is original 2 times, therefore only needs 3 at every turn, within 1st, take 3 times, greatly reduced dose having under the condition of more active component.
The specific embodiment
Form by the following examples, foregoing of the present invention is described in further detail again, but this should be interpreted as to the scope of the above-mentioned theme of the present invention only limits to following example, all technology realized based on foregoing of the present invention all belong to scope of the present invention.
Embodiment 1
Getting Fructus Xanthii (stir-fry) 120g, Radix Saposhnikoviae 90g, Radix Astragali 150g, Radix Angelicae Dahuricae 90g, Flos Magnoliae 90g, Rhizoma Atractylodis Macrocephalae (parched) 90g, Herba Menthae 30g joins in CO2 supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 4%, extracting pressure 15MPa, 30 ℃ of temperature, CO
2flow 1m1/g crude drug min, extraction time 150min, obtain supercritical extract, adds starch, 70% ethanol granule processed, drying, tabletting, make 300, every heavy 0.5g.
Embodiment 2
Get Fructus Xanthii (stir-fry) 120g, Radix Saposhnikoviae 90g, Radix Astragali 150g, Radix Angelicae Dahuricae 90g, Flos Magnoliae 90g, Rhizoma Atractylodis Macrocephalae (parched) 90g, Herba Menthae 30g and join CO
2in the supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 6%, extracting pressure 30MPa, temperature 50 C, CO
2flow 3m1/g crude drug min, extraction time 180min, obtain supercritical extract, adds starch, 70% ethanol granule processed, drying, tabletting, make 300, every heavy 0.5g.
Embodiment 3
Get Fructus Xanthii (stir-fry) 120g, Radix Saposhnikoviae 90g, Radix Astragali 150g, Radix Angelicae Dahuricae 90g, Flos Magnoliae 90g, Rhizoma Atractylodis Macrocephalae (parched) 90g, Herba Menthae 30g and join CO
2in the supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 5%, extracting pressure 20MPa, 40 ℃ of temperature, CO
2flow 2m1/g crude drug min, extraction time 160min, obtain supercritical extract, adds starch, 70% ethanol granule processed, drying, tabletting, make 300, every heavy 0.5g.
Embodiment 4: TONGXIAO BIYAN PIAN suppresses the experimentation data of P815 cell proliferation
1 experiment material
1.1 experiment cell strain
Mouse hypertrophy cell cancer P815 cell, Nanjing Zheng Liang Pharmaceutical Technology Co., Ltd laboratory cell bank, DMEM+10%FBS cellar culture.
1.2 Experimental agents
Drugs: TONGXIAO BIYAN PIAN of the present invention: press embodiment 3 method preparations.
The medicinal liquid liquid storage: take the 100mg TONGXIAO BIYAN PIAN, be dissolved in the 5ml dehydrated alcohol, 0.2 μ m filter filters, 500 μ ldoff pipe packing, and-20 ℃ of storages, 0.2 μ m filter filters the use of dehydrated alcohol in order to matched group simultaneously.
1.3 experiment reagent
The Cat.No.12100-061Lot.No.758137 of DMEM(GIBCO company); Hyclone (Hangzhoupro, sky, Zhejiang bio tech ltd Lot.No.100419); NaHCO3(Shanghai hundred million chemical reagent company limited Cat.No.11810-033Lot.No.1088387 of a specified duration); Trypsin(AMRESCO company lot number: 2010/04); EDTA(AMRESCO company lot number: 2009/10); Penicillin G Sodium Salt(AMRESCO company lot number: 2010242); Streptomycin Sulfate(AMRESCO company lot number: 2010382); Dehydrated alcohol (Nanjing Chemistry Reagent Co., Ltd.'s lot number: 080310182); MTT (Biosharp lot number: 0793); The autogamy of PBS(laboratory);
1.4 experiment equipment
Lycra inverted microscope (German Leica model: DM1L); Visible-ultraviolet light microwell plate detector (U.S. MD company model: SPECTRA MAX190); CO2 incubator (FORMA model: 3111); (safe and sound company of Su Jing group manufactures model to super-clean bench: SW-CJ-ZFD); Pure water instrument (U.S. Spring company model: S/N020579); Accurate pipettor (French Gilson Inc model: P2); Electronic balance (German Sai Duolisi company limited model: BT323S); Full-automatic high-pressure autoclave (Japanese SANYO company model: MLS-3020); Table electrothermal air dry oven (Shanghai accurate experimental facilities company model: DHG9123A); Refrigerator (Siemens Company's model: KG18V21TI); Liquid nitrogen container (CBS model: 2001); Low speed centrifuge (Anting Scientific Instrument Factory, Shanghai's model: KA-1000); 0.2 μ m filter (MILLIPORE model: SLGP033RB); 10cm culture dish (NEST company), 96 well culture plates (NEST company); Cell counting count board; Centrifuge tube, pipet, Tips are some.
2 experimental techniques
1) the P815 cell carries out cellar culture (10cm culture dish) with DMEM+10%FBS in 37 ℃, 5%CO2, when Growth of Cells during to logarithmic (log) phase, collecting cell, discard culture fluid, PBS fine laundering 3 times, add 3ml0.25% trypsin-0.04%EDTA, after 37 ℃ of digestion 2min, add wherein 5ml complete medium neutralization reaction, after the piping and druming cell, it is proceeded in centrifuge tube, the centrifugal 5min of 1000rpm, adjust 3 * 104/ml of concentration of cell suspension.
2) the cell kind is entered in 96 well culture plates, every hole adds cell suspension 180 μ l, culture plate put into cell culture incubator (37 ℃, 5%CO2) cellar culture.
3) according to the Growth of Cells situation, generally grow to 50%-70%, add TONGXIAO BIYAN PIAN solution, continue to cultivate 24h.
4) add 20 μ l MTT solution (5mg/ml, i.e. 0.5%MTT) after 24h, continue to cultivate 4h.
5) after 4h, the buckle method is removed supernatant, with absorbent paper, pats dry gently, and every hole adds 200 μ l dimethyl sulfoxide, puts low-speed oscillation 10min on shaking table, and crystal is fully dissolved.Measure the light absorption value in each hole at enzyme-linked immunosorbent assay instrument 490nm place.
6) background (do not add cell, only add culture fluid) is set simultaneously, control wells (the medicine dissolution medium of cell, same concentrations, culture fluid, MTT, dimethyl sulfoxide), set 6 multiple holes for every group.
7) with medicine, the suppression ratio to cell means result:
Cell increment suppression ratio (%)=(control wells OD value-dosing holes OD value)/control wells OD value * 100%.Experiment repeats 3 times.
3 statistical dispositions
Adopt correlation analysis and Student t check in Microsoft Excel2003 software, data mean with mean ± S.D..
4 experimental results
Statistical result showed after the mtt assay experiment, with matched group, compare, when dosage reaches 5mg/ml, to P815 cell inhibitory effect variant (P<0.05), dosage this difference when 10mg/ml has significance (P<0.01), and utmost point significant difference (P<0.001) is arranged when dosage reaches 15-20mg/ml.
Table 1 TONGXIAO BIYAN PIAN is on P815 cell inhibitory effect impact research
Annotate: with matched group, compare,
*p<0.01;
*p<0.001
5 experiment conclusion
TONGXIAO BIYAN PIAN can suppress the P815 cell proliferation, reduces the Growth of Cells number of P815 cell, and this effect is dose dependent.
Claims (6)
1. the preparation method of a TONGXIAO BIYAN PIAN, by Fructus Xanthii (parched) 120g, Radix Saposhnikoviae 90g, Radix Astragali 150g, Radix Angelicae Dahuricae 90g, Flos Magnoliae 90g, Rhizoma Atractylodis Macrocephalae (parched) 90g, Herba Menthae 30g, as crude drug, made, it is characterized in that described method is comprised of the following step: get Fructus Xanthii (parched) 120g, Radix Saposhnikoviae 90g, Radix Astragali 150g, Radix Angelicae Dahuricae 90g, Flos Magnoliae 90g, Rhizoma Atractylodis Macrocephalae (parched) 90g, Herba Menthae 30g, join CO
2in the supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 4-6%, extracting pressure 15-30MPa, temperature 30-50 ℃, CO
2flow 1-3m1/g crude drug min, extraction time 150-180min, obtain supercritical extract, adds starch, 70% ethanol granule processed, drying, tabletting, make 300, every heavy 0.5g.
2. a kind of preparation method of TONGXIAO BIYAN PIAN according to claim 1, is characterized in that described CO
2the percent by volume that the supercritical extraction entrainer accounts for total extractant is 5%.
3. a kind of preparation method of TONGXIAO BIYAN PIAN according to claim 1, is characterized in that described CO
2the extracting pressure 20MPa of supercritical extraction, 40 ℃ of temperature, CO
2flow 2ml/g crude drug min, extraction time 160min.
4. a kind of TONGXIAO BIYAN PIAN suppresses the application in mouse hypertrophy cell cancer P815 cell proliferation medicine in preparation according to claim 1, it is characterized in that TONGXIAO BIYAN PIAN made as crude drug by Fructus Xanthii (parched) 120g, Radix Saposhnikoviae 90g, Radix Astragali 150g, Radix Angelicae Dahuricae 90g, Flos Magnoliae 90g, Rhizoma Atractylodis Macrocephalae (parched) 90g, Herba Menthae 30g, preparation method is comprised of the following step: get Fructus Xanthii (parched) 120g, Radix Saposhnikoviae 90g, Radix Astragali 150g, Radix Angelicae Dahuricae 90g, Flos Magnoliae 90g, Rhizoma Atractylodis Macrocephalae (parched) 90g, Herba Menthae 30g, join CO
2in the supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 4-6%, extracting pressure 15-30MPa, temperature 30-50 ℃, CO
2flow 1-3m1/g crude drug min, extraction time 150-180min, obtain supercritical extract, adds starch, 70% ethanol granule processed, drying, tabletting, make 300, every heavy 0.5g.
5. a kind of TONGXIAO BIYAN PIAN suppresses the application in mouse hypertrophy cell cancer P815 cell proliferation medicine in preparation according to claim 4, it is characterized in that CO described in the preparation method of TONGXIAO BIYAN PIAN
2the percent by volume that the supercritical extraction entrainer accounts for total extractant is 5%.
6. a kind of TONGXIAO BIYAN PIAN suppresses the application in mouse hypertrophy cell cancer P815 cell proliferation medicine in preparation according to claim 4, it is characterized in that CO described in the preparation method of TONGXIAO BIYAN PIAN
2the extracting pressure 20MPa of supercritical extraction, 40 ℃ of temperature, CO
2flow 2ml/g crude drug min, extraction time 160min.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103919885A (en) * | 2014-04-25 | 2014-07-16 | 云南云龙制药股份有限公司 | Preparation method and application of nasosinusitis soft capsule |
| CN104922227A (en) * | 2015-05-28 | 2015-09-23 | 吉林省正和药业集团股份有限公司 | Preparation method of Yuanhe stomach tablet and application |
| CN106214858A (en) * | 2016-08-26 | 2016-12-14 | 李洪芳 | A kind of medicine treating allergic rhinitis and preparation method thereof |
-
2013
- 2013-10-11 CN CN201310473825.0A patent/CN103494885B/en active Active
Non-Patent Citations (2)
| Title |
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| 孙亮等: "1300大孔吸附树脂分离通窍鼻炎片中总黄酮总皂苷的工艺研究", 《药学实践杂志》, vol. 25, no. 4, 31 December 2007 (2007-12-31), pages 215 - 218 * |
| 常敏毅: "日用抗癌本草", 《医药世界》, no. 3, 31 December 2005 (2005-12-31), pages 82 - 83 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103919885A (en) * | 2014-04-25 | 2014-07-16 | 云南云龙制药股份有限公司 | Preparation method and application of nasosinusitis soft capsule |
| CN104922227A (en) * | 2015-05-28 | 2015-09-23 | 吉林省正和药业集团股份有限公司 | Preparation method of Yuanhe stomach tablet and application |
| CN106214858A (en) * | 2016-08-26 | 2016-12-14 | 李洪芳 | A kind of medicine treating allergic rhinitis and preparation method thereof |
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| Publication number | Publication date |
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| CN103494885B (en) | 2016-01-20 |
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