CN106069189A

CN106069189A - A kind of industrial culture method of seafood mushroom

Info

Publication number: CN106069189A
Application number: CN201610446001.8A
Authority: CN
Inventors: 张家明
Original assignee: TONGCHENG JIAMING AGRICULTURAL DEVELOPMENT Co Ltd
Current assignee: TONGCHENG JIAMING AGRICULTURAL DEVELOPMENT Co Ltd
Priority date: 2016-06-21
Filing date: 2016-06-21
Publication date: 2016-11-09

Abstract

The invention discloses the industrial culture method of a kind of seafood mushroom, relate to agricultural plantation technology field, including plant formulation, bottling, sterilizing cools down, inoculation, send out bacterium, mycelium stimulation, urge flower bud, management of producing mushroom, gathers, classification and preservation and freshness, this industrial culture method, reasonable in design, cost-effective, improve production yields.

Description

A kind of industrial culture method of seafood mushroom

Technical field

The invention belongs to agricultural plantation technology field, be specifically related to the industrial culture method of a kind of seafood mushroom.

Background technology

Seafood mushroom belongs to the raw bacterium of low form grass, is that one has very high nutritive value and medical value, favored by consumers Edible fungi treasure, this mushroom morphological appearance, quality is tender and crisp, delicious flavour, enjoys that " hearing then Tricholoma matsutake (lto et lmai) Singer, food is then beautiful in recent years in Japan Gill fungus " reputation, but the cultural method of the batch production of existing seafood mushroom is unsatisfactory, therefore, occurs that cost is high, the office yielded poorly Face.

Summary of the invention

The present invention provides the industrial culture method of a kind of seafood mushroom, high to solve cost, the problem yielded poorly.

To achieve these goals, the technical scheme that the present invention takes is: the industrial culture method of a kind of seafood mushroom, bag Include following steps:

(1) plant formulation: planting material is made up of corn cob, wheat bran, Semen Maydis powder, Gypsum Fibrosum powder, Calx and water.

(2) bottling: charging bottle uses the vinyon bottle of 800mL, and the weight of content is 550～600g, siccative About 250g, Compost moisture content is 65%.The compost being stirred is delivered to the hopper of bottling machine again by automatically filling by elevator Bottle unit automatic charge, the puncher in regulation bottling unit, make hole can reach at the bottom of bottle 2～3mm.

(3) sterilizing cooling: use high pressure steam sterilization, cools down after sterilizing.

(4) inoculation: batch production inoculation is aseptically to carry out, and goes through whether used strain has during inoculation Living contaminants and undergrowth phenomenon, the cell age of strain is defined in after sending out the full bottle of bacterium and adds 10 again～15d, and with physiological maturity mark As, mycelium cross section is light cream-coloured or light powder oil colours, before inoculation, washs original seeds bottle outer wall with potassium permanganate solution, inoculates personnel Need to dress through over cleaning clothing, footwear and the mask sterilized, by transfer room of being allowed for access after Airshower chamber cleaning, use automatic vaccination Machine is inoculated.

(5) bacterium is sent out: by plastic bottle closure inboard recessed part, strain is pressed into steamed bread shape, and by the strain of surrounding also by covering Sub-inboard is gently pressed, and the temperature in culturing room is adjusted to 20～22 DEG C, uses exchange type ventilation unit to make indoor CO2 concentration Maintain less than 0.4%, incubation keeps dark state.

(6) mycelium stimulation: use special mushroom culturing device, will cultivate charge level central authorities claw type blade and rotate pressure, by culture medium charge level Surrounding is scratched and is removed, and forms circular groove, after mycelium stimulation, needs toward water filling in bacterium bottle, all outwells after 1h.

(7) urge flower bud: make to cover material with porose plastic sheeting etc., indoor carbon dioxide concentration control 0.1%～ 0.2%；Mushroom flower bud early period of origination 1～10lx, mushroom flower bud generation middle and late stage need the illuminance of 50～100lx, carry out tapping mode.

(8) management of producing mushroom: after former base or mushroom flower bud occur, the temperature in cultivation workshop should control at 14～16 DEG C, the wettest Degree controls 90%～95%, mushroom flower bud formed just, illumination in mid-term control 50～100lx, the later stage control 100～ The illumination of 200lx, in cultivation house, every day turns on light 10～15h, turns off the light daytime, turns on light evening or pause switch lamp.

(9) gather: after mycelium stimulation, 20～23d i.e. gather, 1 tide mushroom of only gathering.

(10) classification, preservation and freshness.

Preferably, the formula of described planting material is by the corn cob 82% pulverized, wheat bran 10%, Semen Maydis powder 7%, Gypsum Fibrosum powder 1.2%, Calx 1.8% and water 63% form.

Preferably, the basal plane of described compost is away from bottleneck about 15mm.

Preferably, during described high pressure steam sterilization, first need all to get rid of the cold air in pot, get rid of the side of cold air Method be heating after when on Pressure gauge pointer rise to 0.5kg/cm²Time, open air bleeding valve, be deflated to pointer and still return to the position of " zero " Put, the time of sterilizing be get rid of cold air after, pressure goes back up to 1.5kg/cm²Time just start calculate, now temperature can reach 121～128 DEG C, at this temperature, maintain 1h, after sterilizing, by the cultivation temperature 30 of culture bottle internal cooling to seafood mushroom mycelia DEG C such as the following to be seeded.

Preferably, the described circular groove distance away from bottleneck is 15～20mm.

Use technical scheme, provide the benefit that: raw materials used cheap, production cost is low, use high pressure Steam sterilization, each step checks on and puts in place, 1 tide mushroom of only gathering, and improves the utilization rate of cultivation storehouse, increases and produce stubble, cost Low, yield is high.

Detailed description of the invention

Embodiment one: the industrial culture method of a kind of seafood mushroom, comprises the following steps:

(1) plant formulation: planting material is by the corn cob 82% pulverized, wheat bran 10%, Semen Maydis powder 7%, Gypsum Fibrosum powder 1.2%, Calx 1.8% and water 63% form.

(2) bottling: charging bottle uses the vinyon bottle of 800mL, and the weight of content is 550g, and siccative 250g is left The right side, Compost moisture content is 65%.The compost being stirred is delivered to the hopper of bottling machine again by automatic bottling machine group by elevator Automatic charge, the puncher in regulation bottling unit, make hole can reach at 2mm at the bottom of bottle, the basal plane of compost is away from bottleneck 15mm。

(3) sterilizing cooling: use high pressure steam sterilization, first need all to get rid of the cold air in pot, gets rid of cold air Method be heating after when on Pressure gauge pointer rise to 0.5kg/cm²Time, open air bleeding valve, be deflated to pointer and still return to " zero " Position, the time of sterilizing be get rid of cold air after, pressure goes back up to 1.5kg/cm²Time just start calculate, now temperature can Reach 121 DEG C, at this temperature, maintain 1h, after sterilizing, by the cultivation temperature 30 DEG C of culture bottle internal cooling to seafood mushroom mycelia Such as the following to be seeded.

(4) inoculation: batch production inoculation is aseptically to carry out, and goes through whether used strain has during inoculation Living contaminants and undergrowth phenomenon, the cell age of strain is defined in after sending out the full bottle of bacterium and adds 15d again, and with physiological maturity sign, Mycelium cross section is light cream-coloured or light powder oil colours, before inoculation, washs original seeds bottle outer wall with potassium permanganate solution, and inoculation personnel need to wear Wear through over cleaning clothing, footwear and the mask sterilized, by transfer room of being allowed for access after Airshower chamber cleaning, enter with automatic vaccination machine Row inoculation.

(5) bacterium is sent out: by plastic bottle closure inboard recessed part, strain is pressed into steamed bread shape, and by the strain of surrounding also by covering Sub-inboard is gently pressed, and the temperature in culturing room is adjusted to 20 DEG C, uses exchange type ventilation unit to make indoor CO₂Concentration maintains 0.3%, incubation keeps dark state.

(6) mycelium stimulation: use special mushroom culturing device, will cultivate charge level central authorities claw type blade and rotate pressure, by culture medium charge level Surrounding is scratched and is removed, and forms circular groove, and the circular groove distance away from bottleneck is 15mm, and making charge level is cima shape, after mycelium stimulation, needs note in bacterium bottle Water, all outwells after 1h.

(7) flower bud is urged: making to cover material with porose plastic sheeting etc., indoor carbon dioxide concentration controls 0.2%；Mushroom flower bud Early period of origination 10lx, mushroom flower bud generation middle and late stage need the illuminance of 100lx, carry out tapping mode.

(8) management of producing mushroom: after former base or mushroom flower bud occur, the temperature in cultivation workshop should control at 16 DEG C, relative humidity control System is 90%, and first, illumination in mid-term that mushroom flower bud is formed control at 100lx, and the later stage controls the illumination at 100lx, every in cultivation house It 10h that turns on light, turns off the light daytime, and turn on light in the evening.

(9) gather: after mycelium stimulation, 20d i.e. gathers, 1 tide mushroom of only gathering.

(10) classification, preservation and freshness.

Using above method, the seafood mushroom low cost of production, yield is higher.

Obviously the present invention implements and is not subject to the restrictions described above, as long as have employed method design and the skill of the present invention The improvement of the various unsubstantialities that art scheme is carried out, or the most improved design by the present invention and technical scheme directly apply to it Its occasion, all within protection scope of the present invention.

Claims

1. an industrial culture method for seafood mushroom, comprises the following steps:

(2) bottling: charging bottle uses the vinyon bottle of 800mL, and the weight of content is 550～600g, and siccative 250g is left The right side, Compost moisture content is 65%.The compost being stirred is delivered to the hopper of bottling machine again by automatic bottling machine group by elevator Automatic charge, the puncher in regulation bottling unit, make hole can reach at the bottom of bottle 2～3mm.

(3) sterilizing: use high pressure steam sterilization, cools down after sterilizing.

(4) inoculation: batch production inoculation is aseptically to carry out, and goes through whether used strain has miscellaneous bacteria during inoculation Polluting and undergrowth phenomenon, the cell age of strain is defined in after sending out the full bottle of bacterium and adds 10 again～15d, and with physiological maturity sign, Mycelium cross section is light cream-coloured or light powder oil colours, before inoculation, washs original seeds bottle outer wall with potassium permanganate solution, and inoculation personnel need to wear Wear through over cleaning clothing, footwear and the mask sterilized, by transfer room of being allowed for access after Airshower chamber cleaning, enter with automatic vaccination machine Row inoculation.

(5) bacterium is sent out: by plastic bottle closure inboard recessed part, strain is pressed into steamed bread shape, and by the strain of surrounding also by lid Side is gently pressed, and the temperature in culturing room is adjusted to 20～22 DEG C, uses exchange type ventilation unit to make indoor CO2 concentration maintain Below 0.4%, incubation keeps dark state.

(6) mycelium stimulation: use special mushroom culturing device, will cultivate charge level central authorities claw type blade and rotate pressure, by culture medium charge level surrounding Scratch and remove, form circular groove, after mycelium stimulation, need toward water filling in bacterium bottle, all outwell after 1h.

(7) flower bud is urged: making to cover material with porose plastic sheeting etc., indoor carbon dioxide concentration controls 0.1%～0.2%； Mushroom flower bud early period of origination 1～10lx, mushroom flower bud generation middle and late stage need the illuminance of 50～100lx, carry out tapping mode.

(8) management of producing mushroom: after former base or mushroom flower bud occur, the temperature in cultivation workshop should control at 14～16 DEG C, relative humidity control System is 90%～95%, and first, illumination in mid-term that mushroom flower bud is formed control 50～100lx, and the later stage controls 100～200lx Illumination, in cultivation house, every day turns on light 10～15h, turns off the light daytime, turns on light evening or pause switch lamp.

(10) classification, preservation and freshness.

2. according to the industrial culture method of the seafood mushroom described in claim 1 step (1), it is characterised in that: described cultivation The formula of material is by the corn cob 82% pulverized, wheat bran 10%, Semen Maydis powder 7%, Gypsum Fibrosum powder 1.2%, Calx 1.8% and 63% group of water Become.

3. according to the industrial culture method of the seafood mushroom described in claim 1 step (2), it is characterised in that: described cultivation The basal plane of material is away from bottleneck about 15mm.

4. according to the industrial culture method of the seafood mushroom described in claim 1 step (3), it is characterised in that: described high pressure During steam sterilization, first need all to get rid of the cold air in pot, get rid of cold air method be heating after when on Pressure gauge refer to Pin rises to 0.5kg/cm²Time, to open air bleeding valve, be deflated to pointer and still return to the position of " zero ", the time of sterilizing is to get rid of After cold air, pressure goes back up to 1.5kg/cm²Time just start calculate, now temperature can reach 121～128 DEG C, at this temperature, Maintain 1h, after sterilizing, by such as the following to be seeded for the cultivation temperature 30 DEG C of culture bottle internal cooling to seafood mushroom mycelia.

5. according to the industrial culture method of the seafood mushroom described in claim 1 step (6), it is characterised in that: described circular groove Distance away from bottleneck is 15～20mm.