CN104396564B - A kind of method of utilizing the large pore fungi of polybag substituting stuff cultivation - Google Patents

Abstract

The invention discloses a kind of method of utilizing the large pore fungi of polybag substituting stuff cultivation, it is characterized in that comprising preparation compost, pack sterilizing, inoculated and cultured, management of producing mushroom, urge flower bud period management, educate mushroom period management, the step of gathering, the culture medium adopting in this step is chosen cotton seed hull, willow sawdust is as major ingredient, analysis for soybean powder, corn flour, sorghum flour, calcium carbonate is as auxiliary material, cultivate this large pore fungi by the method for polybag substituting stuff cultivation, this large pore fungi is after my unit domesticating and cultivating success, screen and the experiment and demonstration of cultivation mode by different culture media formula, realize the object that the large pore fungi after domestication can be cultivated in enormous quantities, utilize this culture medium and cultural method, can make the cultivation of large pore fungi high-quality and efficient, output is high, quality better, economic benefit is superior.

Description

A kind of method of utilizing the large pore fungi of polybag substituting stuff cultivation

Technical field

Patent of the present invention relates to a kind of fungus growing technique, especially a kind of method of utilizing the large pore fungi of polybag substituting stuff cultivation.

Background technology

Large pore fungi, Polyporaceae macropore Pseudomonas fungi, is mainly distributed in the ground such as Hebei, Henan, Shanxi, Heilungkiang, Liaoning, Tibet, Zhejiang, Anhui, Hunan, Guangxi, Shaanxi, Gansu, Sichuan, Guizhou, Yunnan.

Macropore Pseudomonas is in Basidiomycotina, Hymenomycetes, Aphyllophorales, Polyporaceae, macropore Pseudomonas; Fructification, without stem or tool short handle, is singly given birth to or closely grows thickly, bacteria cover diameter 5～13cm, fan-shaped, kidney-shaped or semicircle, slightly recessed shallow funnel-form, the smooth surface of being in middle part, shallow khaki, to dirty white, seemingly has ring-type or ring grain, thin edge and slightly involute, bacterial context white, edible when children, meat is tender and crisp, delicious flavour, aging rear approximate cellulosic, the special bacterium fragrance of tool taste. This bacterium fungi polysaccharide, amino acid content enrich, and its extract has to sarcoma S-180 and ehrlich carcinoma the effect of obviously drawing up; A kind of rare food, medicine dual-purpose bacterium that exploitation is worth that have.

Macropore bacterial classification Yuan Xi our company is wild separating obtained, and China Committee for Culture Collection of Microorganisms, the preservation of common micro-organisms center are entrusted, preserving number is CGMCCNo.3711, preservation date: on April 6th, 2010, depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC). Although wild large pore fungi is tamed, its culture technique does not have maturation, and existing culture technique drawback is too many, causes large pore fungi to yield poorly, and quality is bad, and culture medium cost is high, and the economic benefit of cultivating large pore fungi is not fine.

Summary of the invention

For the problems of the prior art; the object of the invention is to provide a kind of method of utilizing the large pore fungi of plastic bag cultivation; the method is arranged on the large pore fungi after domestication in polybag and cultivates, and in conjunction with the special nature of culture medium and special incubation step, has realized the large-scale planting of large pore fungi; and by the combination of this cultural method and compost; large pore fungi grows fine, and output is high, and economic benefit is outstanding; can realize the large pore fungi of bacterium agricultural university large-scale planting, create larger economic worth.

In order to achieve the above object, technical scheme of the present invention is:

A kind of method of utilizing the large pore fungi of polybag substituting stuff cultivation, it is characterized in that comprising the steps: a, preparation compost: select materials (1): choose cotton seed hulls, willow sawdust as major ingredient, analysis for soybean powder, corn flour, sorghum flour, calcium carbonate, as auxiliary material, wherein, calculate according to percentage by weight, cotton seed hulls 45%, willow sawdust 40%, analysis for soybean powder 6%, corn flour 5%, sorghum flour 3%, calcium carbonate 1%, choosing of major ingredient and auxiliary material is material fresh, dry, that nothing is gone mouldy, (2) preparation: first the cotton seed hulls in major ingredient and willow sawdust are mixed, again the analysis for soybean powder in auxiliary material, corn flour, sorghum flour, calcium carbonate are mixed, then the auxiliary material mixing is joined in the major ingredient mixing, again mix, make compost, finally in compound, add water, stir, compost is fully absorbed water, final water content reaches 60%～65%, in the process of this preparation, to note requirement accomplish two even one fully (material and material evenly, material and water evenly, water suction wants abundant), b, pack sterilizing: select wide 17～18cm, long 35～36cm, thickness is at the polyethylene knuckle bag of 0.05～0.06cm, every packed compost 1250～1500g mixing, can adopt mechanical charging, also can adopt artificial pack, the pocket installing requires degree of tightness appropriateness, the remaining 6cm of sack left and right, first in the middle of pocket, make a call to a hole that 5cm is dark with loop-carrier, sack film is inserted in hole, by long 16cm, the loop-carrier with cap enters pocket from hole interpolation again, make the upper cap of the loop-carrier sack that compresses and obturage, then sack downwards or be sidelong in iron sterilizing Turnround basket, whole basket packs in atmospheric steam sterilizing, sterilizing 8～12h under 100 DEG C～102 DEG C conditions of normal pressure, after vexed 4～6h, take the dish out of the pot again, c, inoculated and cultured: (1) inoculation: the pocket after step b is taken the dish out of the pot is transported in clean cooling chamber in time, be cooled to below 30 DEG C, at inoculating hood or indoor, first bacterial classification surface aging mycoderma is removed by sterile working, with inoculating hook, bacterial classification is blended, extract the loop-carrier on pocket, bacterial classification is accessed in excellent hole, the cotton jam-pack after sterilizing for aperture, bacterial classification will fill up excellent hole, the general bottled bacterial classification of 750ml, 25 left and right of bag can connect material, (2) cultivate: postvaccinal bacterium bag is emitted on multi-stage bedstead or culturing rack, layered putting, depending on environment temperature height, put 1～2 layer for every layer, bedstead interlayer will suitably stay a little spaces, be conducive to circulation of air, in whole bacteria developing period, to create suitable condition, to promote the healthy and strong growth of mycelia, in postvaccinal 10 days, mycelia is in sprouting setting date, room temperature should be controlled at 26 DEG C～27 DEG C, impel bacterial classification field planting as early as possible, after 10 days, mycelia has entered vigorous period, because its respiration produces heat, bag in temperature often than room temperature high 2 DEG C～4 DEG C, now room temperature is comparatively suitable with 23 DEG C～24 DEG C, this one-phase, culturing room's temperature is not lower than 20 DEG C or higher than 27 DEG C, and strengthen room ventilation amount, promote the healthy and strong growth of mycelia. through 20 days, bag wall can be seen mycelia, and 30 days, mycelia can be covered with pocket, d, management of producing mushroom: in step c, mycelia is covered with after bag, continue to cultivate 15～20 days, make to move in fruiting canopy after mycelia physiological maturity, code becomes bacterium wall to carry out management of producing mushroom, the management in large pore fungi fruiting stage is to obtain high-quality, high yield, the key of stable yields: (1) mushroom canopy arranges: enter before bag first by mushroom canopy (room) and mushroom producing multi-layer shelf clean out, the fresh pulverized limestone of one deck is evenly spread on ground, if pile up on the spot bacterium bag, need in mushroom canopy, laterally or longitudinally put with fragment of brick wide 25cm～30cm or 50cm～60cm, the wall base of high 5cm～10cm, line-spacing 80cm～100cm, use edible mushroom special sterilizing, pesticide, carry out comprehensively killing one time, desinsection processing, (2) row bag, rinse, sterilize, go plug: by bacterium sack outwards neat traverse on the wall base, height is advisable with 5～7 layers, then with high-pressure spray gun, the bacterium bag two ends water setting is rinsed well, 4～6h ventilates, then Methylpartricin Sodium Laurylsulfate or the bleaching powder aqueous solution to bacterium bag, booth inwall, ceiling and 1:300 of space atomized spray times with sprayer, airtight sterilization 2h～4h, finally removes tampon, the hole of a diameter 2cm of charge level meeting self-assembling formation, e, urge flower bud period management: mushroom temperature of shed is controlled to 16 DEG C～22 DEG C, and relative air humidity is controlled at 85%～95%, gives the scattered light of 800lx～1000lx, adds forced ventilation, keep air fresh, by CO₂Concentration is controlled at below 0.1%, and after 7 days, the former base of warty can form in hole; F, educate mushroom period management: after the former base of warty forms, relative air humidity is controlled to 85%～90%, to aerial, wall, the light atomized water spraying in ground,, cloudy day many by fine day less, the water spray principle regulation and control that stop of rainy day; Temperature is controlled at 16 DEG C～20 DEG C; Scattered light is at 500lx～1000lx; Add forced ventilation, gas concentration lwevel is controlled at below 0.1%; G, gather: large pore fungi buddings and can gather for latter 10 days, in the time that mushroom lid not yet launches by open and flat, spore, is Harvesting Date, and large pore fungi can fruiting 2～3 tides, the first damp mushroom adds forced ventilation after gathering, stop spraying water 4～5 days, make mycelia rehabilitation, and then recover management of producing mushroom, 3 weeks with the interior second damp mushroom of gathering, while adopting mushroom, require to adopt mushroom personnel and cut flat nail, soap clean both hands, dress mushroom cleaning container is clean, and the Methylpartricin Sodium Laurylsulfate aqueous solution with 0.1% is sterilized or liquid soap is cleaned, dried.

Further, gathering in step, while specifically gathering, pinch cap base portion with hand, break down gently or with lancinating down.

Further, if adopt warmhouse booth cultivate large pore fungi, can divide spring, autumn two season cultivation, spring cultivation can be at bag in 2～March, 4～June fruiting; Autumn cultivation can be at bag in August, 9～October fruiting.

The cultivation technique of large pore fungi is also immature at present, this large pore fungi is after my unit domesticating and cultivating success, screen and the experiment and demonstration of cultivation mode by different culture media formula, having explored and having utilized cotton seed hulls, sawdust is major ingredient, analysis for soybean powder, corn flour, sorghum flour etc. are the High-yield Formula of auxiliary material and the ripe cultural method that utilizes polybag, further realize the object that the large pore fungi after domestication can be cultivated in enormous quantities, utilize this culture medium and cultural method, can make the cultivation of large pore fungi high-quality and efficient, output is high, quality better, economic benefit is superior.

Detailed description of the invention

Below in conjunction with embodiment, the present invention is further described.

Embodiment mono-:

A kind of method of utilizing the large pore fungi of polybag substituting stuff cultivation, it is characterized in that comprising the steps: a, preparation compost: select materials (1): choose cotton seed hulls, willow sawdust as major ingredient, analysis for soybean powder, corn flour, sorghum flour, calcium carbonate, as auxiliary material, wherein, calculate according to percentage by weight, cotton seed hulls 45%, willow sawdust 40%, analysis for soybean powder 6%, corn flour 5%, sorghum flour 3%, calcium carbonate 1%, choosing of major ingredient and auxiliary material is material fresh, dry, that nothing is gone mouldy, (2) preparation: first the cotton seed hulls in major ingredient and willow sawdust are mixed, again the analysis for soybean powder in auxiliary material, corn flour, sorghum flour, calcium carbonate are mixed, then the auxiliary material mixing is joined in the major ingredient mixing, again mix, make compost, finally in compound, add water, stir, compost is fully absorbed water, final water content reaches 60%～65%, in the process of this preparation, to note requirement accomplish two even one fully (material and material evenly, material and water evenly, water suction wants abundant), b, pack sterilizing: select wide 17～18cm, long 35～36cm, thickness is at the polyethylene knuckle bag of 0.005～0.006cm, every packed compost 1250～1500g mixing, can adopt mechanical charging, also can adopt artificial pack, the pocket installing requires degree of tightness appropriateness, the remaining 6cm of sack left and right, first in the middle of pocket, make a call to a hole that 5cm is dark with loop-carrier, sack film is inserted in hole, by long 16cm, the loop-carrier with cap enters pocket from hole interpolation again, make the upper cap of the loop-carrier sack that compresses and obturage, then sack downwards or be sidelong in iron sterilizing Turnround basket, whole basket packs in atmospheric steam sterilizing, sterilizing 8～12h under 100 DEG C～102 DEG C conditions of normal pressure, after vexed 4～6h, take the dish out of the pot again, c, inoculated and cultured: (1) inoculation: the pocket after step b is taken the dish out of the pot is transported in clean cooling chamber in time, be cooled to below 30 DEG C, at inoculating hood or indoor, first bacterial classification surface aging mycoderma is removed by sterile working, with inoculating hook, bacterial classification is blended, extract the loop-carrier on pocket, bacterial classification is accessed in excellent hole, the cotton jam-pack after sterilizing for aperture, bacterial classification will fill up excellent hole, the general bottled bacterial classification of 750ml, 25 left and right of bag can connect material, (2) cultivate: postvaccinal bacterium bag is emitted on multi-stage bedstead or culturing rack, layered putting, depending on environment temperature height, put 1～2 layer for every layer, bedstead interlayer will suitably stay a little spaces, be conducive to circulation of air, in whole bacteria developing period, to create suitable condition, to promote the healthy and strong growth of mycelia, in postvaccinal 10 days, mycelia is in sprouting setting date, room temperature should be controlled at 26 DEG C～27 DEG C, impel bacterial classification field planting as early as possible, after 10 days, mycelia has entered vigorous period, because its respiration produces heat, bag in temperature often than room temperature high 2 DEG C～4 DEG C, now room temperature is comparatively suitable with 23 DEG C～24 DEG C, this one-phase, culturing room's temperature is not lower than 20 DEG C or higher than 27 DEG C, and strengthen room ventilation amount, promote the healthy and strong growth of mycelia. through 20 days, bag wall can be seen mycelia, and 30 days, mycelia can be covered with pocket, d, management of producing mushroom: in step c, mycelia is covered with after bag, continue to cultivate 15～20 days, make to move in fruiting canopy after mycelia physiological maturity, code becomes bacterium wall to carry out management of producing mushroom, the management in large pore fungi fruiting stage is to obtain high-quality, high yield, the key of stable yields: (1) mushroom canopy arranges: enter before bag first by mushroom canopy (room) and mushroom producing multi-layer shelf clean out, the fresh pulverized limestone of one deck is evenly spread on ground, if pile up on the spot bacterium bag, need in mushroom canopy, laterally or longitudinally put with fragment of brick wide 25cm～30cm or 50cm～60cm, the wall base of high 5cm～10cm, line-spacing 80cm～100cm, use edible mushroom special sterilizing, pesticide, carry out comprehensively killing one time, desinsection processing, (2) row bag, rinse, sterilize, go plug: by bacterium sack outwards neat traverse on the wall base, height is advisable with 5～7 layers, then with high-pressure spray gun, the bacterium bag two ends water setting is rinsed well, 4～6h ventilates, then Methylpartricin Sodium Laurylsulfate or the bleaching powder aqueous solution to bacterium bag, booth inwall, ceiling and 1:300 of space atomized spray times with sprayer, airtight sterilization 2h～4h, finally removes tampon, the hole of a diameter 2cm of charge level meeting self-assembling formation, e, urge flower bud period management: mushroom temperature of shed is controlled to 16 DEG C～22 DEG C, and relative air humidity is controlled at 85%～95%, gives the scattered light of 800lx～1000lx, adds forced ventilation, keep air fresh, by CO₂Concentration is controlled at below 0.1%, and after 7 days, the former base of warty can form in hole; F, educate mushroom period management: after the former base of warty forms, relative air humidity is controlled to 85%～90%, to aerial, wall, the light atomized water spraying in ground,, cloudy day many by fine day less, the water spray principle regulation and control that stop of rainy day; Temperature is controlled at 16 DEG C～20 DEG C; Scattered light is at 500lx～1000lx; Add forced ventilation, gas concentration lwevel is controlled at below 0.1%; G, gather: large pore fungi buddings and can gather for latter 10 days, in the time that mushroom lid not yet launches by open and flat, spore, is Harvesting Date, and large pore fungi can fruiting 2～3 tides, the first damp mushroom adds forced ventilation after gathering, stop spraying water 4～5 days, make mycelia rehabilitation, and then recover management of producing mushroom, 3 weeks with the interior second damp mushroom of gathering, while adopting mushroom, require to adopt mushroom personnel and cut flat nail, soap clean both hands, dress mushroom cleaning container is clean, and the Methylpartricin Sodium Laurylsulfate aqueous solution with 0.1% is sterilized or liquid soap is cleaned, dried.

Embodiment bis-:

A kind of method of utilizing the large pore fungi of polybag substituting stuff cultivation, it is characterized in that comprising the steps: a, preparation compost: select materials (1): choose cotton seed hulls, willow sawdust as major ingredient, analysis for soybean powder, corn flour, sorghum flour, calcium carbonate, as auxiliary material, wherein, calculate according to percentage by weight, cotton seed hulls 45%, willow sawdust 40%, analysis for soybean powder 6%, corn flour 5%, sorghum flour 3%, calcium carbonate 1%, choosing of major ingredient and auxiliary material is material fresh, dry, that nothing is gone mouldy, (2) preparation: first the cotton seed hulls in major ingredient and willow sawdust are mixed, again the analysis for soybean powder in auxiliary material, corn flour, sorghum flour, calcium carbonate are mixed, then the auxiliary material mixing is joined in the major ingredient mixing, again mix, make compost, finally in compound, add water, stir, compost is fully absorbed water, final water content reaches 65%, in the process of this preparation, to note requirement accomplish two even one fully (material and material evenly, material and water evenly, water suction wants abundant), b, pack sterilizing: select wide 17～18cm, long 35～36cm, the thick polyethylene knuckle bag at 0.005～0.006cm of bag, every packed compost 1500g mixing, can adopt mechanical charging, also can adopt artificial pack, the pocket installing requires degree of tightness appropriateness, the remaining 6cm of sack left and right, first in the middle of pocket, make a call to a hole that 5cm is dark with loop-carrier, sack film is inserted in hole, by long 16cm, the loop-carrier with cap enters pocket from hole interpolation again, make the upper cap of the loop-carrier sack that compresses and obturage, then sack downwards or be sidelong in iron sterilizing Turnround basket, whole basket packs in atmospheric steam sterilizing, sterilizing 10h under 100 DEG C of conditions of normal pressure, after vexed 45h, take the dish out of the pot again, c, inoculated and cultured: (1) inoculation: the pocket after step b is taken the dish out of the pot is transported in clean cooling chamber in time, be cooled to below 30 DEG C, at inoculating hood or indoor, first bacterial classification surface aging mycoderma is removed by sterile working, with inoculating hook, bacterial classification is blended, extract the loop-carrier on pocket, bacterial classification is accessed in excellent hole, the cotton jam-pack after sterilizing for aperture, bacterial classification will fill up excellent hole, the general bottled bacterial classification of 750ml, 25 left and right of bag can connect material, (2) cultivate: postvaccinal bacterium bag is emitted on multi-stage bedstead or culturing rack, layered putting, depending on environment temperature height, put 1～2 layer for every layer, bedstead interlayer will suitably stay a little spaces, be conducive to circulation of air, in whole bacteria developing period, to create suitable condition, to promote the healthy and strong growth of mycelia, in postvaccinal 10 days, mycelia is in sprouting setting date, room temperature should be controlled at 26 DEG C～27 DEG C, impel bacterial classification field planting as early as possible, after 10 days, mycelia has entered vigorous period, because its respiration produces heat, bag in temperature often than room temperature high 2 DEG C～4 DEG C, now room temperature is comparatively suitable with 23 DEG C～24 DEG C, this one-phase, culturing room's temperature is not lower than 20 DEG C or higher than 27 DEG C, and strengthen room ventilation amount, promote the healthy and strong growth of mycelia. through 20 days, bag wall can be seen mycelia, and 30 days, mycelia can be covered with pocket, d, management of producing mushroom: in step c, mycelia is covered with after bag, continue to cultivate 18 days, make to move in fruiting canopy after mycelia physiological maturity, code becomes bacterium wall to carry out management of producing mushroom, the management in large pore fungi fruiting stage is to obtain high-quality, high yield, the key of stable yields: (1) mushroom canopy arranges: enter before bag first by mushroom canopy (room) and mushroom producing multi-layer shelf clean out, the fresh pulverized limestone of one deck is evenly spread on ground, if pile up on the spot bacterium bag, need in mushroom canopy, laterally or longitudinally put with fragment of brick wide 50cm～60cm, the wall base of high 5cm～10cm, line-spacing 80cm～100cm, use edible mushroom special sterilizing, pesticide, carry out comprehensively killing one time, desinsection processing, (2) row bag, rinse, sterilize, go plug: by bacterium sack outwards neat traverse on the wall base, height is advisable with 5～7 layers, then with high-pressure spray gun, the bacterium bag two ends water setting is rinsed well, ventilation 5h, then Methylpartricin Sodium Laurylsulfate or the bleaching powder aqueous solution to bacterium bag, booth inwall, ceiling and 1:300 of space atomized spray times with sprayer, airtight sterilization 3h, finally removes tampon, the hole of a diameter 2cm of charge level meeting self-assembling formation, e, urge flower bud period management: mushroom temperature of shed is controlled to 16 DEG C～22 DEG C, and relative air humidity is controlled at 85%～95%, gives the scattered light of 800lx～1000lx, adds forced ventilation, keep air fresh, by CO₂Concentration is controlled at below 0.1%, and after 7 days, the former base of warty can form in hole; F, educate mushroom period management: after the former base of warty forms, relative air humidity is controlled to 85%～90%, to aerial, wall, the light atomized water spraying in ground,, cloudy day many by fine day less, the water spray principle regulation and control that stop of rainy day; Temperature is controlled at 16 DEG C～20 DEG C; Scattered light is at 500lx～1000lx; Add forced ventilation, gas concentration lwevel is controlled at below 0.1%; G, gather: large pore fungi buddings and can gather for latter 10 days, in the time that mushroom lid not yet launches by open and flat, spore, is Harvesting Date, and large pore fungi can fruiting 2～3 tides, the first damp mushroom adds forced ventilation after gathering, stop spraying water 4～5 days, make mycelia rehabilitation, and then recover management of producing mushroom, 3 weeks with the interior second damp mushroom of gathering, while adopting mushroom, require to adopt mushroom personnel and cut flat nail, soap clean both hands, dress mushroom cleaning container is clean, and the Methylpartricin Sodium Laurylsulfate aqueous solution with 0.1% is sterilized or liquid soap is cleaned, dried.

Be understandable that, above about specific descriptions of the present invention, only for being described, the present invention is not limited to the described technical scheme of the embodiment of the present invention, those of ordinary skill in the art is to be understood that, still can modify or be equal to replacement the present invention, to reach identical technique effect; Use needs as long as meet, all within protection scope of the present invention.

Claims (5)

1. one kind is utilized the method for the large pore fungi of polybag substituting stuff cultivation, it is characterized in that comprising the steps: a, preparation compost: select materials (1): choose cotton seed hulls, willow sawdust as major ingredient, analysis for soybean powder, corn flour, sorghum flour, calcium carbonate, as auxiliary material, wherein, calculate according to percentage by weight, cotton seed hulls 45%, willow sawdust 40%, analysis for soybean powder 6%, corn flour 5%, sorghum flour 3%, calcium carbonate 1%, (2) preparation: first the cotton seed hulls in major ingredient and willow sawdust are mixed, again the analysis for soybean powder in auxiliary material, corn flour, sorghum flour, calcium carbonate are mixed, then the auxiliary material mixing is joined in the major ingredient mixing, again mix, make compost, finally in compound, add water, stir, compost is fully absorbed water, and final water content reaches 60%～65%, b, pack sterilizing: select wide 17～18cm, long 35～36cm, thickness is at the polyethylene knuckle bag of 0.005～0.006cm, every packed compost 1250～1500g mixing, the pocket installing requires degree of tightness appropriateness, the remaining 6cm of sack left and right, first in the middle of pocket, make a call to a hole that 5cm is dark with loop-carrier, sack film is inserted in hole, by long 16cm, the loop-carrier with cap enters pocket from hole interpolation again, make the upper cap of the loop-carrier sack that compresses and obturage, then sack downwards or be sidelong in iron sterilizing Turnround basket, whole basket packs in atmospheric steam sterilizing, sterilizing 8～12h under 100 DEG C～102 DEG C conditions of normal pressure, after vexed 4～6h, take the dish out of the pot again, c, inoculated and cultured: (1) inoculation: the pocket after step b is taken the dish out of the pot is transported in clean cooling chamber in time, be cooled to below 30 DEG C, at inoculating hood or indoor, first bacterial classification surface aging mycoderma is removed by sterile working, with inoculating hook, bacterial classification is blended, extract the loop-carrier on pocket, bacterial classification is accessed in excellent hole, the cotton jam-pack after sterilizing for aperture, (2) cultivate: postvaccinal bacterium bag is emitted on multi-stage bedstead or culturing rack, layered putting, depending on environment temperature height, put 1～2 layer for every layer, bedstead interlayer will suitably stay a little spaces, be conducive to circulation of air, in postvaccinal 10 days, mycelia is in sprouting setting date, room temperature should be controlled at 26 DEG C～27 DEG C, impel bacterial classification field planting as early as possible, after 10 days, mycelia has entered vigorous period, because its respiration produces heat, bag in temperature often than room temperature high 2 DEG C～4 DEG C, now 23 DEG C～24 DEG C of room temperatures, this one-phase, culturing room's temperature is not lower than 20 DEG C or higher than 27 DEG C, and strengthen room ventilation amount, promote the healthy and strong growth of mycelia,

Through 20 days, bag wall can be seen mycelia, and 30 days, mycelia can be covered with pocket; D, management of producing mushroom: in step c, mycelia is covered with after bag, continue to cultivate 15～20 days, make to move in fruiting canopy after mycelia physiological maturity, code becomes bacterium wall to carry out management of producing mushroom: (1) mushroom canopy arranges: enter before bag first by mushroom canopy and mushroom producing multi-layer shelf clean out, the fresh pulverized limestone of one deck is evenly spread on ground, if pile up on the spot bacterium bag, need in mushroom canopy, laterally or longitudinally put with fragment of brick wide 25cm～30cm or 50cm～60cm, the wall base of high 5cm～10cm, line-spacing 80cm～100cm, with edible mushroom special sterilizing, pesticide, carry out comprehensively killing, desinsection processing; (2) row bag, rinse, sterilize, go plug: by bacterium sack outwards neat traverse on the wall base, be highly 5～7 layers, then with high-pressure spray gun, the bacterium bag two ends water setting is rinsed well, 4～6h ventilates, then Methylpartricin Sodium Laurylsulfate or the bleaching powder aqueous solution to bacterium bag, booth inwall, ceiling and 1:300 of space atomized spray times with sprayer, airtight sterilization 2h～4h, finally removes tampon, the hole of a diameter 2cm of charge level meeting self-assembling formation; E, urge flower bud period management: mushroom temperature of shed is controlled to 16 DEG C～22 DEG C, and relative air humidity is controlled at 85%～95%, gives the scattered light of 800lx～1000lx, adds forced ventilation, keep air fresh, by CO₂Concentration is controlled at below 0.1%, and after 7 days, the former base of warty can form in hole; F, educate mushroom period management: after the former base of warty forms, relative air humidity is controlled to 85%～90%, to aerial, wall, the light atomized water spraying in ground,, cloudy day many by fine day less, the water spray principle regulation and control that stop of rainy day; Temperature is controlled at 16 DEG C～20 DEG C; Scattered light is at 500lx～1000lx; Add forced ventilation, gas concentration lwevel is controlled at below 0.1%; G, gather: large pore fungi buddings and can gather for latter 10 days, in the time that mushroom lid not yet launches by open and flat, spore, for Harvesting Date, and large pore fungi fruiting 2～3 tides, the first damp mushroom adds forced ventilation after gathering, stop spraying water 4～5 days, make mycelia rehabilitation, and then recover management of producing mushroom, 3 weeks with the interior second damp mushroom of gathering.

2. a kind of method of utilizing the large pore fungi of polybag substituting stuff cultivation according to claim 1, is characterized in that: gathering in step, while specifically gathering, pinch cap base portion with hand, break down gently or with lancinating down.

3. a kind of method of utilizing the large pore fungi of polybag substituting stuff cultivation according to claim 1, is characterized in that: in step a in compost major ingredient and auxiliary material choose be fresh, dry, without the material going mouldy.

4. a kind of method of utilizing the large pore fungi of polybag substituting stuff cultivation according to claim 1, is characterized in that: when inoculation, bacterial classification will fill up the excellent hole of loop-carrier.

5. a kind of method of utilizing the large pore fungi of polybag substituting stuff cultivation according to claim 1, is characterized in that: adopt warmhouse booth to cultivate large pore fungi, point spring, autumn two season cultivation, the spring is planted in bag in 2～March, 4～June fruiting; Autumn is planted in bag in August, 9～October fruiting.