CN106011041A - Composite lactobacillus preparation used for increasing tomato quality and preparation method thereof - Google Patents
Composite lactobacillus preparation used for increasing tomato quality and preparation method thereof Download PDFInfo
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Abstract
The invention provides a composite lactobacillus preparation used for increasing tomato quality and a preparation method there, which belong to the field of microbe application. The composite lactobacillus preparation is composed of lactobacillus plantarum P-8, lactobacillus casei Zhang and Lactobacillus delbrueckii subsp. bulgaricus according to proportion of (1.8-2.4): (1.8-2.4): (0.9-1.3), and the live bacteria total content of the composite lactobacillus preparation is 2.4-3.2*109 cfu/ml. The complex formulation of three bacteria has obvious promotion effect on growth and metabolism of tomato, especially can cooperate and promote the lycopene and Vc content of the tomato, increases tomato quality, and increases the plantation benefit.
Description
Technical field
The present invention relates to microbe application field, particularly relate to a kind of for promoting the compound of Tomato Quality
Lactic acid bacteria microbial inoculum and preparation method.
Background technology
Fructus Lycopersici esculenti (Lycopersicon esculentum) is that one of the most universal fruit and vegetable are cultivated, not in the whole world
Name Fructus Lycopersici esculenti, originates in from South America, and the extensively cultivation of the South China north, tamato fruit is nutritious, contains
There are abundant vitamin C, lycopene and vitamin B group etc..Development of Vegetable Industry is rapid in recent years,
Tomato planting area constantly expands, along with the raising of people's quality of life, to tomato yield and quality also
Have higher requirement.And because of blindly to enrich fertilizer in producing, especially chemical fertilizer, it is thus achieved that high yield
Become a kind of universal phenomenon, excess use chemical fertilizer production cost can be caused to increase considerably, soil matter
The problems such as amount is degenerated and environmental pollution is serious, have had influence on agricultural products in China safety and agricultural are raw
The sustainable development produced.
Microbial-bacterial fertilizer is a kind of new bio-fertilizer after fertilizer and chemical fertilizer, and numerous studies are tied
Fruit shows, microbial manure has high efficiency, nonhazardous, the feature such as pollution-free, not only can reduce agriculture
Contamination of products, and can improving quality, the most in the world the country of nearly half include Japan, Britain,
Developed country that the U.S., France, Sweden etc. are main and the developing china such as Pakistan, India, China
Family, all in the development and utilization carrying out microbial manure.Yin Xianhui etc. study discovery, dilute 200 times
Microbial bacterial agent process best to the facilitation effect of Development of Tomato, yield and quality.Jiang Lili
Find Deng microorganisms bacterial manure application effect on greenhouse tomato, with compareing of using common chemical fertilizer
Compare, use Toyota's treasured microbial-bacterial fertilizer effect of increasing production by 750kg/ mu and 1 000kg/ mu good, economical
Remarkable benefit.Meanwhile, it is capable to reduce the pollution of chemical fertilizer, improve fruit quality.
Lactic acid bacteria is as a kind of safe microorganism generally existed in natural environment, in the kind of agricultural product
Plant and with the obvious advantage in quality safety (phosphorus is to denitrogenate the second largest plant nutrient outside element in soil,
Although total phosphorus content is high in soil, but plant cannot utilize.The lactic acid of lactic acid bacteria secretion, acetic acid etc. one
Aspect can directly dissolve insoluble phosphate in soil, the most then be unearthed by chelation release
Earth phosphorus element, in order to plant absorption utilizes, and is beneficial to the raising of plant products and quality).Without pesticide
The agricultural product more and more welcomed by the people used with chemical fertilizer, lactic acid bacteria is in agricultural planting and agriculture
The application of product quality security fields is more extensive, but can not effectively facilitate at present kind
The lactic acid bacteria microbial inoculum that eggplant quality improves.
Summary of the invention
It is an object of the invention to by probing into the different lactic acid bacteria growth traits to Fructus Lycopersici esculenti and the shadow of quality
Ring, determine the suitable lactobacillus preparation of application in Fructus Lycopersici esculenti, it is provided that one is used for promoting Tomato Quality
Compound lactobacillus microbial inoculum and preparation method thereof.
In order to realize the purpose of the present invention, adopt the following technical scheme that
The present invention is the compound lactobacillus microbial inoculum for promoting Tomato Quality, and this compound lactobacillus microbial inoculum is by plant
Lactobacillus (Lactobacillus plantarum) P-8, lactobacillus casei (Lactobacillus casei) Zhang
With lactobacillus delbruockii subspecies bulgaricus (Lactobacillus delbrueckii subsp.bulgaricus) group
Become.
It should be noted that Lactobacillus plantarum of the present invention (Lactobacillus plantarum)
P-8, is isolatable from the clabber of Inner Mongol traditional natural fermentation, has the anti-gastrointestinal tract Digestive system of excellence
Tolerance, it is possible to survive in animal intestinal；This bacterial strain has been preserved in Chinese microorganism strain preservation
Administration committee's General Microbiological Culture preservation center, preserving number is CGMCC No.6312；The present invention
Lactobacillus casei (Lactobacillus casei) Zhang used, is the resistance to of separation from koumiss
The probiotics strain of sour and resistance to bile acid；This bacterial strain is preserved in Chinese micro-life on April 21st, 2006
Thing culture presevation administration committee's General Microbiological Culture preservation center, preserving number CGMCC
No.1697.Lactobacillus delbruockii subspecies bulgaricus (Lactobacillus delbrueckii subsp.
Bulgaricus) being referred to as Lactobacillus bulgaricus, have the ability of leaven, it is sweet from Gansu Province
In the traditional zymotic yak milk of Tibetan autonomous prefecture, south, screening obtains；During on April 22nd, 2011 is preserved in
Microbiological Culture Collection administration committee of state General Microbiological Culture preservation center, preserving number CGMCC
No.4774。
Preferably, compound lactobacillus microbial inoculum as above, this microbial inoculum is by Lactobacillus plantarum
(Lactobacillus plantarum) P-8 and lactobacillus casei (Lactobacillus casei) Zhang
Press with lactobacillus delbruockii subspecies bulgaricus (Lactobacillus delbrueckii subsp.bulgaricus)
(1.8-2.4): (1.8-2.4): the ratio composition of (0.9-1.3), wherein, Lactobacillus plantarum (Lactobacillus
Plantarum) ratio of P-8 and lactobacillus casei (Lactobacillus casei) Zhang is 1:1.
Preferably, Lactobacillus plantarum (Lactobacillus plantarum) P-8 and lactobacillus casei
(Lactobacillus casei) Zhang and lactobacillus delbruockii subspecies bulgaricus (Lactobacillus
Delbrueckiisubsp.bulgaricus) mix in the ratio of 2:2:1.
Total viable count content 2.4-3.2 × 10 of described compound lactobacillus microbial inoculum9cfu/ml。
Preferably, the total viable count of described compound lactobacillus microbial inoculum is 3 × 109cfu/ml。
Another aspect of the present invention provides the preparation method of above-mentioned compound lactobacillus microbial inoculum, and concrete grammar walks
Rapid as follows:
(1) first by described Lactobacillus plantarum (Lactobacillus plantarum) P-8 and cheese milk bar
Bacterium (Lactobacillus casei) Zhang is inoculated in MRS culture fluid, under the conditions of 37 ± 1 DEG C
Cultivate 26-28 hour, with physiological saline solution centrifuge washing 3 times, the bacterial sediment obtained adds
8%-12% sterilized non-fat emulsion, and to adjust its bacterium number be 1.8-2.0 × 109Cfu/ml, after mixing
Cultivate 35-48 hour under the conditions of 35-37 DEG C；
(2) strain culture step (1) obtained and lactobacillus delbruockii subspecies bulgaricus
(Lactobacillus delbrueckii subsp.bulgaricus) mixes in proportion, is inoculated in MRS
Culture fluid, cultivates 12-24 hour under the conditions of 39 ± 1 DEG C, and adds the coconut juice of 50ml/l, adjusts
Its bacterium number is 2.4-3.2 × 109Cfu/ml, pH value is 5.5-6.5.
Preferably, step (1) Lactobacillus plantarum (Lactobacillus plantarum) P-8 and cheese milk
The inoculum concentration of bacillus (Lactobacillus casei) Zhang is respectively 4-10% (v/w).
Preferably, step (1) Lactobacillus plantarum (Lactobacillus plantarum) P-8 and cheese milk
The inoculum concentration of bacillus (Lactobacillus casei) Zhang is 1:1.
Preferably, step (1) obtains strain culture and lactobacillus delbruockii subspecies bulgaricus
(Lactobacillus delbrueckii subsp.bulgaricus) is in (3.6-4.8): the ratio of (0.9-1.3) is entered
Row mixing, and it is inoculated in MRS culture medium with the inoculum concentration of 8-12% (v/w).
Present invention also offers this compound lactobacillus microbial inoculum application on Fructus Lycopersici esculenti, bloom spring from Fructus Lycopersici esculenti
Bearing fruit the phase, by 2L/ mu, 2 times/month are watered with water and are imposed on plant root.
The invention has the beneficial effects as follows, the composite bacteria agent capable that the present invention relates to is by Lactobacillus plantarum
(Lactobacillus plantarum) P-8, lactobacillus casei (Lactobacillus casei) Zhang
With lactobacillus delbruockii subspecies bulgaricus (Lactobacillus delbrueckii subsp.bulgaricus) group
Becoming, the compounding growth metabolism to Fructus Lycopersici esculenti of these three bacterium has obvious facilitation, it is particularly possible to collaborative
Promoting the yield and quality characters of Fructus Lycopersici esculenti, single plant yield reaches 2.44kg/ strain, and relatively blank group improves
12.96%；Lycopene reaches 0.68g/kg, and relatively blank group improves 28.25%；Fructus Lycopersici esculenti
Vc relatively blank group improves 27.01%；Soluble sugar and the content of soluble solid, more right
22.09% and 17.60% have been respectively increased it according to group.Serve the effect of obvious upgrading volume increase, and then
Increase planting benefit.
Accompanying drawing explanation
Fig. 1: the different lactic acid bacterias single fruit weight on Fructus Lycopersici esculenti and the impact of single plant yield；
Fig. 2: different lactic acid bacterias are on the lycopene of Fructus Lycopersici esculenti and the impact of Vc content；
Fig. 3: different lactic acid bacterias are on Fructus Lycopersici esculenti soluble sugar and the impact of soluble solid；
Detailed description of the invention
Technical scheme in the embodiment of the present invention will be clearly and completely described below, it is clear that
Described embodiment is only a part of embodiment of the present invention rather than whole embodiments.Based on this
Embodiment in invention, those of ordinary skill in the art are obtained under not making creative work premise
The every other embodiment obtained, broadly falls into the scope of protection of the invention.
On the one hand the embodiment of the present invention provides a kind of compound lactobacillus microbial inoculum for promoting Tomato Quality,
This compound lactobacillus microbial inoculum is by Lactobacillus plantarum (Lactobacillus plantarum) P-8, lactobacillus casei
(Lactobacillus casei) Zhang and lactobacillus delbruockii subspecies bulgaricus (Lactobacillus
Delbrueckii subsp.bulgaricus) composition.
It should be noted that the Lactobacillus plantarum used (Lactobacillus plantarum) P-8,
It is isolatable from the clabber of Inner Mongol traditional natural fermentation, there is the anti-gastrointestinal tract Digestive system tolerance of excellence
Ability, it is possible to survive in animal intestinal；This bacterial strain has been preserved in Chinese microorganism strain preservation management
Committee's General Microbiological Culture preservation center, preserving number is CGMCC No.6312；The present invention is adopted
Lactobacillus casei (Lactobacillus casei) Zhang, be from koumiss separate acidproof and
The probiotics strain of resistance to bile acid；This bacterial strain is preserved in China Microbiological bacterium on April 21st, 2006
Plant preservation administration committee General Microbiological Culture preservation center, preserving number CGMCC No.1697.Moral
Family name's lactobacillus subspecies bulgaricus (Lactobacillus delbrueckii subsp.bulgaricus), has
The ability of leaven, it is to screen to obtain from the traditional zymotic yak milk of Tibetan Autonomous Prefecture of Gannan, Gansu Province
's；On April 22nd, 2011 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms
DSMZ, preserving number CGMCC No.4774.
In one embodiment of this invention, this compound lactobacillus microbial inoculum is by Lactobacillus plantarum
(Lactobacillus plantarum) P-8 and lactobacillus casei (Lactobacillus casei) Zhang
Press with lactobacillus delbruockii subspecies bulgaricus (Lactobacillus delbrueckii subsp.bulgaricus)
(1.8-2.4): (1.8-2.4): the ratio composition of (0.9-1.3), wherein, Lactobacillus plantarum (Lactobacillus
Plantarum) ratio of P-8 and lactobacillus casei (Lactobacillus casei) Zhang is 1:1,
Total viable count content 2.4-3.2 × 10 of described compound lactobacillus microbial inoculum9cfu/ml。
Concrete, the ratio of three kinds of bacterium can be 1.8:1.8:0.9,1.9:1.9:1.0,2:2:1,2.1:2.1:1.1,
2.2:2.2:1.1,2.3:2.3:1.2,2.4:2.4:1.3, but it is understood that, the ratio of three kinds of bacterium can
To adjust the numerical value of any appropriate within the above range, it is not limited to aforementioned proportion.
In a preferred embodiment of the present invention, Lactobacillus plantarum (Lactobacillus
Plantarum) P-8 and lactobacillus casei (Lactobacillus casei) Zhang and Deshi Lactobacillus are protected
Add Leah subspecies (Lactobacillus delbrueckii subsp.bulgaricus) to mix in the ratio of 2:2:1
Closing, the total viable count of described composite bacteria agent capable is 3 × 109cfu/ml。
Further embodiment of this invention provides the preparation method of above-mentioned compound lactobacillus microbial inoculum, concrete grammar
Step is as follows:
S1: first by described Lactobacillus plantarum (Lactobacillus plantarum) P-8 and cheese milk
The inoculum concentration of the bacillus each 4-10% of (Lactobacillus casei) Zhang (v/w) is inoculated in MRS
In culture medium, cultivate 26-28 hour under the conditions of 37 ± 1 DEG C, with physiological saline solution centrifuge washing 3
Secondary, the bacterial sediment obtained adds 8%-12% sterilized non-fat emulsion, and to adjust its bacterium number be 1.8-2.0
×109Cfu/ml, cultivates after mixing and cultivates 35-48 hour under the conditions of 35-37 DEG C.
In this step, it is possible to fully cultivate above two bacterium, it is to avoid antagonism.
S2: the strain that above-mentioned steps is obtained and lactobacillus delbruockii subspecies bulgaricus (Lactobacillus
Delbrueckii subsp.bulgaricus) in (3.6-4.8): (0.9-1.3) ratio mixes, and with
The inoculum concentration of 8-12% (v/w) is inoculated in MRS culture medium, cultivates 12-24 under the conditions of 39 ± 1 DEG C
Hour, and add the coconut juice of 50ml/l, adjusting its bacterium number is 2.4-3.2 × 109Cfu/ml, pH value is
5.5-6.5。
In this step, add coconut juice to strengthen nutrition with anti-browning, thus ensure three kinds of microbial inoculums
Quickly effectively cultivate, it is to avoid the antagonism between strain, effectively raise the activity of microbial inoculum.
Another embodiment of the present invention is this compound lactobacillus microbial inoculum application on Fructus Lycopersici esculenti, from the Fructus Lycopersici esculenti spring
Ji Kaihua bears fruit the phase, and by 2L/ mu, 2 times/month are watered with water and imposed on plant root.
The composite bacteria agent capable that the these three lactic acid bacteria that the embodiment of the present invention is provided the compounds growth generation to Fructus Lycopersici esculenti
Thank to obvious facilitation, it is particularly possible to the collaborative lycopene promoting Fructus Lycopersici esculenti and Vc content, carried
Rise the quality of Fructus Lycopersici esculenti, and then increase planting benefit.
In order to become apparent from introducing in detail, the embodiment of the present invention provided for promoting answering of Tomato Quality
Close lactic acid bacteria microbial inoculum and preparation method, illustrate below with reference to specific embodiment.
Embodiment 1: this compound lactobacillus microbial inoculum is by Lactobacillus plantarum (Lactobacillus plantarum)
P-8 and lactobacillus casei (Lactobacillus casei) Zhang and lactobacillus delbruockii subspecies bulgaricus
(Lactobacillus delbrueckii subsp.bulgaricus) is formed in the ratio of 1.8:1.8:0.9,
The total viable count content 2.4 × 10 of described compound lactobacillus microbial inoculum9cfu/ml。
Embodiment 2: this compound lactobacillus microbial inoculum is by Lactobacillus plantarum (Lactobacillus plantarum)
P-8 and lactobacillus casei (Lactobacillus casei) Zhang and lactobacillus delbruockii subspecies bulgaricus
(Lactobacillus delbrueckii subsp.bulgaricus) is formed in the ratio of 2.4:2.4:1.3,
The total viable count content 2.8 × 10 of described compound lactobacillus microbial inoculum9cfu/ml。
Embodiment 3: this compound lactobacillus microbial inoculum is by Lactobacillus plantarum (Lactobacillus plantarum)
P-8 and lactobacillus casei (Lactobacillus casei) Zhang and lactobacillus delbruockii subspecies bulgaricus
(Lactobacillus delbrueckii subsp.bulgaricus) is formed in the ratio of 2:2:1, described
The total viable count content 3.2 × 10 of compound lactobacillus microbial inoculum9cfu/ml。
The impact of tomato growth is tested by different lactic acid bacterias
Test period: in June, 2016-July；Test site: Qingdao Agricultural University practice base；Examination
Test material: ' MAOFEN 802 ' is as test material with Fructus Lycopersici esculenti.
Packet situation is:
(1) blank group: spray clear water；
(2) lactobacillus delbruockii subspecies bulgaricus: spray lactobacillus delbruockii subspecies bulgaricus, viable bacteria
Sum content 0.8-1.0 × 109cfu/ml；
(3) compound bacteria 1: spray on plants lactobacillus+lactobacillus casei bacterium solution, total viable count content
1.8-2.0×109Cfu/ml, the quantity of two kinds of bacterium ratio for 1:1, adds the dilution of MRS culture fluid during use,
Making total viable count is 0.9-1.0 × 109cfu/ml
(4) compound bacteria 2: spray on plants lactobacillus+lactobacillus casei liquid+Lactobacillus delbrueckii
Subspecies, total viable count content 2.4-3.2 × 109Cfu/ml, the quantity of three kinds of bacterium is than for 2:2:1, during use
Adding the dilution of MRS culture fluid, making total viable count is 0.8-1.05 × 109cfu/ml
Blooming the phase of bearing fruit from Fructus Lycopersici esculenti, by 2L/ mu, 2 times/month are watered with water and are imposed on plant root in spring.
Measure each process single fruit weight on Fructus Lycopersici esculenti and the impact of single plant yield, lycopene and Vc respectively
Content, Fructus Lycopersici esculenti soluble sugar and the content of soluble solid, concrete data see table 1 below.
The impact on Fructus Lycopersici esculenti indices of table 1 lactic acid bacteria
Note: compound bacteria 1 is Lactobacillus plantarum+lactobacillus casei, compound bacteria 2 be Lactobacillus plantarum+lactobacillus casei+
Lactobacillus bulgaricus；Compare with blank group,▲P ＜ 0.01；Compound bacteria 2 compares with Lactobacillus bulgaricus group,P ＜ 0.01；Compound bacteria 2 compares with compound bacteria 1 group,■P ＜ 0.01.
Lactic acid bacteria as shown in Fig. 1, table 1, applies the Fructus Lycopersici esculenti of 3 kinds of lactic acid bacterias on the impact of tomato yield
Its single fruit weight relatively blank group has been respectively increased 3.05%, 4.57%, 11.17%；3 kinds of lactic acid bacterias pair
The impact of Fructus Lycopersici esculenti single plant yield is the most more apparent, and especially compound bacteria 2 is to its effect maximum, reaches 2.44
Kg/ strain, relatively blank group (2.16kg/ strain) improves 12.96%.Compound bacteria 2 compares compound bacteria
1 or the difference of Lactobacillus bulgaricus or matched group all have extremely significantly statistical significance (P ＜ 0.01).
As shown in Fig. 2, table 1,3 kinds of lactic acid bacterias are more blank right to lycopene and the Vc content of Fructus Lycopersici esculenti
All having raising in various degree according to group, wherein compound bacteria 2 is more Bulgarian to the effect of its lycopene
Substantially, lycopene reaches 0.68g/kg, relatively blank group (5.31g/kg) for lactobacillus and compound bacteria 1
Improve 28.25%.3 kinds of lactic acid bacterias have been respectively increased 12.08% to Fructus Lycopersici esculenti Vc relatively blank group,
13.93%, 27.01%.Compound bacteria 2 compares the difference of compound bacteria 1, Lactobacillus bulgaricus or comparison
All there is extremely significantly statistical significance (P ＜ 0.01).
As shown in Fig. 3, table 1, compound bacteria 2 is to Fructus Lycopersici esculenti soluble sugar and the lifting of soluble solid
Effect maximum, relatively matched group has been respectively increased 22.09% and 17.60%.Lactobacillus bulgaricus, multiple
Close bacterium 1 and the impact relatively blank group of Fructus Lycopersici esculenti soluble sugar be respectively increased 10.68%, 11.89%,
The impact relatively blank group of Fructus Lycopersici esculenti soluble solid has been respectively increased 9.86% and 11.22%, because of
These 3 kinds of lactic acid bacterias show as compound bacteria 2 to the impact of Fructus Lycopersici esculenti soluble sugar and soluble solid > compound
Bacterium 1 > Lactobacillus bulgaricus > blank.
From above-mentioned Fig. 1-3 and table 1, can be seen that different lactic acid bacteria is different on the impact of tomato growth,
Aggregate performance is compound bacteria 2 > compound bacteria 1 > Lactobacillus bulgaricus > blank group.Result shows,
Lactobacillus plantarum (Lactobacillus plantarum) P-8, lactobacillus casei (Lactobacillus casei)
Zhang and lactobacillus delbruockii subspecies bulgaricus (Lactobacillus delbrueckii subsp.
Bulgaricus) raising of active component content in promotion Fructus Lycopersici esculenti can be worked in coordination with, energy is increased production in Fructus Lycopersici esculenti upgrading
Play obvious facilitation.
Claims (9)
1. for promoting the compound lactobacillus microbial inoculum of Tomato Quality, it is characterised in that this compound lactobacillus microbial inoculum
By Lactobacillus plantarum (Lactobacillus plantarum) P-8, lactobacillus casei (Lactobacillus casei)
Zhang and lactobacillus delbruockii subspecies bulgaricus (Lactobacillus delbrueckii subsp.
Bulgaricus) in (1.8-2.4): (1.8-2.4): the ratio composition of (0.9-1.3), wherein Lactobacillus plantarum
(Lactobacillus plantarum) P-8 and lactobacillus casei (Lactobacillus casei) Zhang
Ratio be 1:1.
Compound lactobacillus microbial inoculum for promoting Tomato Quality the most according to claim 1, its feature exists
In, Lactobacillus plantarum (Lactobacillus plantarum) P-8 and lactobacillus casei (Lactobacillus
Casei) Zhang and lactobacillus delbruockii subspecies bulgaricus (Lactobacillus delbrueckii subsp.
Bulgaricus) mix in the ratio of 2:2:1.
Compound lactobacillus microbial inoculum for promoting Tomato Quality the most according to claim 1, its feature exists
In, the total viable count content of described compound lactobacillus microbial inoculum is 2.4-3.2 × 109cfu/ml。
Compound lactobacillus microbial inoculum for promoting Tomato Quality the most according to claim 3, its feature exists
In, described in meet the total viable count of microbial inoculum be 3 × 109cfu/ml。
5. the preparation of the compound lactobacillus microbial inoculum for promoting Tomato Quality described in any one of claim 1-4
Method, it is characterised in that specifically comprise the following steps that
(1) by described Lactobacillus plantarum (Lactobacillus plantarum) P-8 and lactobacillus casei
(Lactobacillus casei) Zhang is inoculated in MRS culture medium, trains under the conditions of 37 ± 1 DEG C
Support 26-28 hour, with physiological saline solution centrifuge washing 3 times, the bacterial sediment obtained adds
8%-12% sterilized non-fat emulsion, and to adjust its bacterium number be 1.8-2.0 × 109Cfu/ml, after mixing
Cultivate 35-48 hour under the conditions of 35-37 DEG C；
(2) strain culture step (1) obtained and lactobacillus delbruockii subspecies bulgaricus
(Lactobacillus delbrueckii subsp.bulgaricus) mixes in proportion, is inoculated in MRS
Culture medium, cultivates 12-24 hour under the conditions of 39 ± 1 DEG C, and adds the coconut juice of 50ml/l, adjusts
Its bacterium number is 2.4-3.2 × 109Cfu/ml, pH value is 5.5-6.5.
The preparation method of the compound lactobacillus microbial inoculum for promoting Tomato Quality the most according to claim 5,
It is characterized in that, step (1) Lactobacillus plantarum (Lactobacillus plantarum) P-8 and cheese milk
The inoculum concentration of bacillus (Lactobacillus casei) Zhang is respectively 4-10% (v/w).
The preparation method of the compound lactobacillus microbial inoculum for promoting Tomato Quality the most according to claim 6,
It is characterized in that, step (1) Lactobacillus plantarum (Lactobacillus plantarum) P-8 and cheese milk
The inoculum concentration of bacillus (Lactobacillus casei) Zhang is 1:1.
The preparation method of the compound lactobacillus microbial inoculum for promoting Tomato Quality the most according to claim 5,
It is characterized in that, the strain that step (1) obtains and lactobacillus delbruockii subspecies bulgaricus (Lactobacillus
Delbrueckii subsp.bulgaricus) in (3.6-4.8): the ratio of (0.9-1.3) mixes, and with
The inoculum concentration of 8-12% (v/w) is inoculated in MRS culture medium.
9. the application of the compound lactobacillus microbial inoculum for promoting Tomato Quality described in any one of claim 1-4,
It is characterized in that, bloom the phase of bearing fruit from Fructus Lycopersici esculenti in spring, by 2L/ mu, 2 times/month are watered with water and are imposed on plant roots
Portion.
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| CN111567261A (en) * | 2020-05-14 | 2020-08-25 | 中国科学院沈阳应用生态研究所 | Plant toner and application and use method thereof |
| CN112573956A (en) * | 2020-12-25 | 2021-03-30 | 东北农业大学 | Seed dressing agent for promoting crop rooting and application thereof |
| CN114350531A (en) * | 2021-12-24 | 2022-04-15 | 青岛尚德生物技术有限公司 | Compound microbial agent for promoting color change of tomatoes and preparation method and application thereof |
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| CN114668007A (en) * | 2022-05-09 | 2022-06-28 | 仲恺农业工程学院 | Fermentation liquor type plant growth regulator and preparation method and application thereof |
| CN114836339A (en) * | 2022-04-08 | 2022-08-02 | 内蒙古中峰生物科技有限公司 | Lactic acid bacteria preparation capable of improving yield and quality of hot pepper and application thereof |
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| CN111567261A (en) * | 2020-05-14 | 2020-08-25 | 中国科学院沈阳应用生态研究所 | Plant toner and application and use method thereof |
| CN111567261B (en) * | 2020-05-14 | 2022-03-04 | 中国科学院沈阳应用生态研究所 | Plant toner and application and use method thereof |
| CN112573956A (en) * | 2020-12-25 | 2021-03-30 | 东北农业大学 | Seed dressing agent for promoting crop rooting and application thereof |
| CN114350531A (en) * | 2021-12-24 | 2022-04-15 | 青岛尚德生物技术有限公司 | Compound microbial agent for promoting color change of tomatoes and preparation method and application thereof |
| CN114403220A (en) * | 2021-12-24 | 2022-04-29 | 昆明生物制造研究院有限公司 | Lactobacillus bubble water and preparation method thereof |
| CN114350531B (en) * | 2021-12-24 | 2023-11-24 | 青岛尚德生物技术有限公司 | Compound microbial agent for promoting color conversion of tomatoes as well as preparation method and application thereof |
| CN114403220B (en) * | 2021-12-24 | 2024-03-22 | 昆明生物制造研究院有限公司 | Lactic acid bacteria bubble water and preparation method thereof |
| CN114885768A (en) * | 2022-03-30 | 2022-08-12 | 杭州市富阳区农业技术推广中心 | Continuous cropping obstacle sustainable prevention and control method for solanaceous vegetables |
| CN114836339A (en) * | 2022-04-08 | 2022-08-02 | 内蒙古中峰生物科技有限公司 | Lactic acid bacteria preparation capable of improving yield and quality of hot pepper and application thereof |
| CN114836339B (en) * | 2022-04-08 | 2024-02-06 | 内蒙古中峰生物科技有限公司 | Lactic acid bacteria preparation capable of improving yield and quality of capsicum and application thereof |
| CN114668007A (en) * | 2022-05-09 | 2022-06-28 | 仲恺农业工程学院 | Fermentation liquor type plant growth regulator and preparation method and application thereof |
| CN114668007B (en) * | 2022-05-09 | 2023-11-21 | 仲恺农业工程学院 | Fermentation broth type plant growth regulator and preparation method and application thereof |
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| CN106011041B (en) | 2019-06-07 |
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