CN105948838A - Compound lactic acid bacteria microbial fertilizer and preparation and application thereof - Google Patents
Compound lactic acid bacteria microbial fertilizer and preparation and application thereof Download PDFInfo
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Abstract
The invention discloses a compound lactic acid bacteria microbial fertilizer, and belongs to the technical field of fertilizers. The biological fertilizer is prepared by fermentation with four strains of lactobacillus plantarum P-8, PB-1, PY-1 and SCI-02. With mixed lactobacillus fermentation, special composition and ratio, the ability of the whole fermentation system to produce phenyllactic acid can be improved, a risk of plants infected with diseases is further reduced in a crop planting process, and the disease aggravating process is delayed or alleviated; the plant yield is increased, the sweetness is increased, and the mouthfeel is improved; beneficial microorganisms in soil are increased, soil borne disease fungi are suppressed, and a soil micro-ecological environment is improved; and moreover, a role of the stress resistance of the crops to the harsh environment is improved.
Description
Technical field:
The invention belongs to technical field of fertilizers, be specifically related to the preparation of a kind of compound lactobacillus microbial manure and answer
With.
Background technology:
Lactic acid bacteria, as the member in agricultural benefit microorganism and Plant growth-promoting effect antibacterial, is universal in natural environment
A kind of safety existed and edible microorganism, play exclusive excellent in agricultural cultivation and quality safety thereof
Gesture.Along with pathogenic fungus characteristic research anti-to lactic acid bacteria gos deep into and lactic acid bacteria degraded heavy metal, pesticide residues
Deng the exploration of safety aspect research, the vegetalitas agricultural product reducing pesticide and chemical fertilizer plantation are got over by people
Carry out the most concerns.Microorganism synergism intrinsic in microbial ecological agent and soil can suppress foot end
Pathogenic fungus, bacterial pathogens and nematicide, the infringement that plant is caused by food grass insecticide even on the ground.Agricultural
The compound probiotic system that useful microbial ecological agent is made up of lactic acid bacteria, yeast, photosynthetic bacteria and actinomycetes etc.
Agent.Said preparation has in promotion plant health growth, increase plant products and photosynthetic rate, acceleration soil organic
Matter is decomposed and the release of nutrient substance, beneficially plant absorption, minimizing chemical agent use, improve soil quality etc.
Effect.
Lactic acid bacteria, as probiotic bacteria, is the class in Plant growth-promoting effect antibacterial, the safe level being well recognized as (GRAS)
Bacterial strain, research finds, Lactobacillus plantarum, lactobacillus rhamnosus, bacillus acidophilus and Deshi Lactobacillus are micro-lifes
Lactobacillus inoculation conventional in thing fertilizer production.Fructus Mali pumilae exists Lactobacillus, Pediococcus and Leuconostoc etc.
Lactic acid bacteria.The lactic acid bacteria on Fructus Vitis viniferae surface is about 100CFU/g, is gradually lowered along with the prolongation of transporting procedures.
In different plants and agricultural product, the research of isolation identification lactic acid bacteria finds, lactic acid bacteria be maximum bacterial population (about
Account for 30%), wherein Lactococcus is superiority bacteria spp.Lactic acid bacteria is applied in vegetalitas agricultural product have suppression and causes
Pathogenic bacteria, the propagation of putrefaction bacteria, degrade heavy metal, pesticide residues, the use etc. reducing pesticide and chemical fertilizer is all
Many advantages, and be more and more valued by the people.
The present invention is directed to above-mentioned problem, to the Lactobacillus plantarum being isolatable from natural plants surface and rhizosphere, carry out
Produce antibacterial substance, the suppression series of experiments such as rot fungus and pathogenic bacterium, filter out 1 strain and there is suppression plant
Pathomycete and common food pollute the Lactobacillus plantarum L.plantarum SCI-02 of pathogen.Then with have
3 strains of lactic acid bacteria (L.plantarum P-8, L.plantarum PB-1, the L. of good rot fungi rejection ability
Plantarum PY-1) mixed fermentation, it is prepared as compound lactobacillus micro-organism fertilizer.Research compound lactobacillus is micro-
Ecological fertilizer is improving soil microenvironment, is promoting plant growing, regulation and control plant disease, enhancing plant stress-resistance
Property and improve the application of the aspects such as vegetalitas quality of agricultural product, it is intended to promote that lactic acid bacteria application in agricultural product is sent out
Exhibition, and improve agricultural product quality and edible safety thereof, to preferably improving the health level of people.
Summary of the invention:
A kind of compound lactobacillus microbial manure, described compound lactobacillus is provided in order to solve the problems referred to above present invention
The preparation method of microbial manure is as follows:
(1) it is that 7% access is sent out by four lactobacillus plantarum P-8, PB-1, PY-1 and SCI-02 by total inoculum concentration
Ferment culture medium is fermented, and fermentation condition is as follows: the initial pH of fermentation tank is 7.0, and fermentation temperature is 30 DEG C, permanent
PH 5.7-5.9, after stream adds alkali liquor 4-5h, after natural fermentation to pH 4.3-4.7, stream adds alkali liquor and maintains fermentation liquid permanent
PH 5.7-5.9 to fermentation liquid self pH is fermentation termination when not changing；
(2), after having fermented, compound lactobacillus-fermencucumber liquid is mixed with the sucrose after aseptic process and Semen sojae atricolor powder
Uniformly i.e. obtain compound lactobacillus microbial manure；
The inoculative proportion of described P-8, PB-1, PY-1 and SCI-02 is: 1:1:1:2；
Described fermentation medium composition is as follows: soybean protein powder 20-40g, sucrose 40-80g, citric acid 0.7-1.0g,
K2HPO41-4g, sodium citrate 8-12g, tween 80 1-3g, distilled water 1L, pH 7.0；
The addition of described sucrose and fermentation liquid mass volume ratio are 0.1%；
The addition of described Semen sojae atricolor powder and fermentation liquid mass volume ratio are 0.1%；
Described Lactobacillus plantarum P-8, deposit number CGMCC No.6312；
Described Lactobacillus plantarum PB-1, deposit number CGMCC No.5399；
Described Lactobacillus plantarum PY-1, deposit number CGMCC No.5358
Described Lactobacillus plantarum SCI-02 is specially Lactobacillus plantarum (Lactobacillus plantarum) SCI-02,
This bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms on 24th in December in 2015
Center, address is: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica, postal
Compile 100101, deposit number CGMCC No.11931；
Described SCI-02 bacterial strain is by suppressing phytopathogenic fungi (aspergillus parasiticus, Aspergillus flavus, penicillium roqueforti, Fructus Lycopersici esculenti
Early epidemic, Fructus Melo are withered, Fructus Mali pumilae anthrax, Fructus Melo phytophthora) and enteropathic antibacterial (escherichia coli, Mus hinder sramana
Salmonella, shigella flexneri, staphylococcus aureus, unit cell listeria spp) screening, and can generation
Thank to high yield broad-spectrum antibacterial material phenyllactic acid；
Described P-8, PB-1, PY-1 bacterial strain has good control effect to common Sapromyces.
Table 1 L.plantarum SCI-02 fungus inhibition test
Table 2 L.plantarum SCI-02 malignant bacteria inhibition test
The technical scheme two that the present invention provides is that above-mentioned compound lactobacillus microbial manure is applied to crops life
In product, specific as follows: every 15-30 days, foliage-spray is according to 100-200ml/ mu/time (by the dilution of 100 times of fertilizer
After use), root pouring according to 500-1000ml/ mu/time (by 100 times of fertilizer dilute after use).
Beneficial effect:
1, being added with L.plantarum SCI-02 in Microecological compound fertilizer material provided by the present invention, this bacterial strain has
There are good bacteriostasis property, and high yield phenyllactic acid.
2, the present invention uses lactobacillus mixed fermentation, special prescription and proportioning can provide high L.plantarum
SCI-02 and whole fermentation system produce the ability of phenyllactic acid.
3, the Microecological compound fertilizer material a. that the present invention provides has the risk reducing plant introduced disease, to plant
There is protective effect, can delay or alleviate disease and increase the weight of process；B. have and make plant increase production, increase sugariness and (contain
Sugar amount increases), improve the effect of mouthfeel；C. be conducive to improving beneficial microbe in soil, suppress soil-borne disease
Fungus, improves the effect of soil microenvironment: reduce fungi in soil；Increase actinomycetes in soil,
Bacillus cereus and total number of bacteria increase (increasing by 6.88%, 10.70% and 46.06% respectively)；D. crop pair is improved
The resistance of adverse circumstances.
Accompanying drawing explanation
Fig. 1: compound lactobacillus microbial manure can reduce the risk of cucumber plant introduced disease
Fig. 2: compound lactobacillus microbial manure can delay cucumber plant disease to increase the weight of process
Fig. 3: compound lactobacillus microbial manure can prevent, alleviate cucumber plant disease
Fig. 4: compound lactobacillus microbial manure can improve tomato production
Fig. 5: compound lactobacillus microbial manure can improve western red sugar content, improve Fructus Lycopersici esculenti taste quality and
Soil physical and chemical property
Fig. 6: compound lactobacillus microbial manure can significantly improve western red plant root development growth
Fig. 7: compound lactobacillus microbial manure can remarkably promote tomato plant growth
Fig. 8: compound lactobacillus microbial manure can improve soil microenvironment
Fig. 9: compound lactobacillus microbial manure can promote that soil with organic matter is decomposed and nitrogen phosphorus nutrients release
Detailed description of the invention:
Below in conjunction with embodiment, the invention will be further described；Following example are the most illustrative, the present invention
It is not limited to these embodiments restrictions.
Experimental technique in following embodiment, if no special instructions, is conventional method.
Compound lactobacillus (L.plantarum P-8, L.plantarum PB-1, L.plantarum PY-1 and L.
Plantarum SCI-02) mixed fermentation prepares microbial manure and application thereof.
Embodiment 1, test method
1.1 actication of culture
The bacterial strain L.plantarum P-8 of lyophilization preservation, L.plantarum PB-1, L.plantarum PY-1
It is inoculated in MRS fluid medium with L.plantarum SCI-02 strain, 37 DEG C of constant temperature culture 18h, passes
Generation 2 times.
1.2 count plate
General employing pour plate counting method, operating procedure is: according to national standard method, shake up fermentation liquid, draws
1mL fermentation liquid is placed in the physiological saline solution of 9mL, carries out 10 times of dilutions, is diluted to institute the most successively
Need gradient.By finally dilute gradient tube dilution shake even after, draw 1mL diluent pour into flat board, arrive
Enter culture medium, shake up, put solidification, cultivate 48h in 37 DEG C of incubators.Flat board does two gradients respectively, each
Gradient two is parallel.
Mensuration viable count of lactobacillus culture medium: MRS solid medium
1.3pH measures: use accurate pH meter directly to measure fermentation liquid pH value
1.4 phenyllactic acid assays (LC-MS method): LC condition: chromatographic column BEH C18；Stream 0.4ml/min；
Column temperature: 45 DEG C；Sample size: 4 μ l；Mobile phase A: water+0.1% formic acid；Mobile phase B: acetonitrile+0.1% first
Acid；Gradient elution 3min.MS condition: positive ion mode, ESI ion source, taper hole voltage 20V；Collision
Can 4V.
The impact on mixed fungus fermentation of embodiment 2 inoculative proportion
Research different vaccination ratio, the impact on strain mixed fermentation.
Culture medium prescription is as follows: soybean protein powder 30g, sucrose 60g, citric acid 0.8g, K2HPO42g, lemon
Lemon acid sodium 10g, tween 80 2g, distilled water 1L, pH 7.0；
The initial pH of fermentation tank: be 7.0, fermentation temperature is 30 DEG C, permanent pH 5.7-5.9, and stream adds alkali liquor and maintains fermentation liquid
Permanent pH 5.7-5.9 to fermentation liquid self pH is fermentation termination when no longer changing；
A. as L.plantarum P-8, L.plantarum PB-1, L.plantarum PY-1 and L.plantarum
Total inoculum concentration of SCI-02 is 6% (inoculative proportion 1:1:1:1), 30 DEG C, and 80r/min ferments, and measures lactic acid bacteria and lives
Bacterium number is 0.72 × 1010CFU/mL；
B. as L.plantarum P-8, L.plantarum PB-1, L.plantarum PY-1 and L.plantarum
Total inoculum concentration of SCI-02 is 6% (inoculative proportion 2:1:1:1), 30 DEG C, and 80r/min ferments, and measures lactic acid bacteria and lives
Bacterium number is 0.84 × 1010CFU/mL；
C. as L.plantarum P-8, L.plantarum PB-1, L.plantarum PY-1 and L.plantarum
Total inoculum concentration of SCI-02 is 7% (inoculative proportion 1:1:1:2), 30 DEG C, and 80r/min ferments, and measures lactic acid bacteria and lives
Bacterium number is 1.03 × 1010CFU/mL；
According to experimental result, as L.plantarum P-8, L.plantarum PB-1, L.plantarum PY-1
Being 7% (inoculative proportion 1:1:1:2) with total inoculum concentration of L.plantarum SCI-02, beneficially compound lactobacillus is micro-
The fermentation of bio-feritlizer.
Embodiment 3 fermentation technology is on the impact of phenyllactic acid in fermentation liquid
Research different fermentations technique, on the impact of antibacterial substance (phenyllactic acid) in mixed fermentation liquid:
Fermentation medium: soybean protein powder 30g, sucrose 60g, citric acid 0.8g, K2HPO42g, Fructus Citri Limoniae
Acid sodium 10g, tween 80 2g, distilled water 1L, pH 7.0.L.plantarum P-8、L.plantarum PB-1、
Total inoculum concentration of L.plantarum PY-1 and L.plantarum SCI-02 is 7% (inoculative proportion 1:1:1:2)；
A. the initial pH of fermentation tank is 7.0, and fermentation temperature is 30 DEG C, permanent pH 5.9, and omnidistance stream adds alkali liquor to sending out
Self pH of ferment liquid is fermentation termination when no longer changing；Broad-spectrum antibacterial material benzene breast in sampling detection fermentation liquid
Acid content is 25.7mg/L, and viable count is 1.10 × 1010CFU/mL；
Other conditions are constant, access strain and change L.plantarum SCI-02 inoculum concentration into when being 7%, experiment knot
Fruit is: phenyllactic acid content is 8.6mg/L, and viable count is 1.01 × 1010CFU/mL。
Other conditions are constant, access strain and change L.plantarum PY-1 inoculum concentration into when being 7%, experimental result
For: phenyllactic acid content is 3.2mg/L, and viable count is 0.98 × 1010CFU/mL
Other conditions are constant, access strain and change L.plantarum P-8 inoculum concentration into when being 7%, and experimental result is:
Phenyllactic acid content is 8.3mg/L, and viable count is 1.21 × 1010CFU/mL
Other conditions are constant, access strain and change L.plantarum PB-1 inoculum concentration into when being 7%, experimental result
For: phenyllactic acid content is 3.7mg/L, and viable count is 0.87 × 1010CFU/mL
B. the initial pH of fermentation tank is 7.0, and fermentation temperature is 30 DEG C, permanent pH 5.9, after stream adds alkali liquor 7h,
Natural fermentation to pH no longer change time (about pH4.2-4.5) be considered as fermentation termination；Sampling detection fermentation liquid
In broad-spectrum antibacterial material phenyllactic acid content be 30.6mg/L, viable count is 0.72 × 1010CFU/mL；
Other conditions are constant, access strain and change L.plantarum SCI-02 inoculum concentration into when being 7%, experiment knot
Fruit is: phenyllactic acid content is 10.3mg/L, and viable count is 0.71 × 1010CFU/mL；
Other conditions are constant, access strain and change L.plantarum PY-1 kind amount into when being 7%, and experimental result is:
Phenyllactic acid content is 5.2mg/L, and viable count is 0.78 × 1010CFU/mL；
Other conditions are constant, access strain and change L.plantarum P-8 kind amount into when being 7%, and experimental result is:
Phenyllactic acid content is 8.1mg/L, and viable count is 0.65 × 1010CFU/mL；
Other conditions are constant, access strain and change L.plantarum PB-1 kind amount into when being 7%, and experimental result is:
Phenyllactic acid content is 4.7mg/L, and viable count is 0.72 × 1010CFU/mL。
C. the initial pH of fermentation tank is 7.0, and fermentation temperature is 30 DEG C, permanent pH 5.9, after stream adds alkali liquor 4.5h,
Natural fermentation is to after pH 4.5, and stream adds alkali liquor and maintains fermentation liquid perseverance pH 5.9 to fermentation liquid self pH no longer to become
It it is fermentation termination during change.Broad-spectrum antibacterial material phenyllactic acid content in sampling detection fermentation liquid is 49.2mg/L,
Viable count is 1.06 × 1010CFU/mL；
Other conditions are constant, access strain and change L.plantarum SCI-02 kind amount into when being 7%, experimental result
For: phenyllactic acid content is 15.3mg/L, and viable count is 0.96 × 1010CFU/mL；
Other conditions are constant, access strain and change L.plantarum PY-1 kind amount into when being 7%, and experimental result is:
Phenyllactic acid content is 10.1mg/L, and viable count is 0.78 × 1010CFU/mL；
Other conditions are constant, access strain and change L.plantarum P-8 kind amount into when being 7%, and experimental result is:
Phenyllactic acid content is 12.4mg/L, and viable count is 1.01 × 1010CFU/mL；
Other conditions are constant, access strain and change L.plantarum PB-1 kind amount into when being 7%, and experimental result is:
Phenyllactic acid content is 9.8mg/L, and viable count is 0.79 × 1010CFU/mL。
D. the initial pH of fermentation tank is 7.0, and fermentation temperature is 30 DEG C, and natural fermentation 24h is terminal.Sampling inspection
The broad-spectrum antibacterial material phenyllactic acid content surveyed in fermentation liquid is 50.1mg/L, and viable count is 0.50 × 1010
CFU/mL；
Other conditions are constant, access strain and change L.plantarum SCI-02 kind amount into when being 7%, experimental result
For: phenyllactic acid content is 12.5mg/L, and viable count is 0.45 × 1010CFU/mL；
Other conditions are constant, access strain and change L.plantarum PY-1 kind amount into when being 7%, and experimental result is:
Phenyllactic acid content is 12.1mg/L, and viable count is 0.56 × 1010CFU/mL；
Other conditions are constant, access strain and change L.plantarum P-8 kind amount into when being 7%, and experimental result is:
Phenyllactic acid content is 9.4mg/L, and viable count is 0.42 × 1010CFU/mL；
Other conditions are constant, access strain and change L.plantarum PB-1 kind amount into when being 7%, and experimental result is:
Phenyllactic acid content is 10.1mg/L, and viable count is 0.51 × 1010CFU/mL。
Conclusion: in sum, through different vaccination ratio, different fermentations technical study, it is considered to 4 strains of lactic acid bacteria mix
When closing fermentation, on viable count of lactobacillus and the impact of antibacterial substance yield, the most preferably: L.plantarum P-8,
Total inoculum concentration of L.plantarum PB-1, L.plantarum PY-1 and L.plantarum SCI-02 is 7% (to connect
Ratio 1:1:1:2 of kind)
The initial pH of fermentation tank is 7.0, and fermentation temperature is 30 DEG C, permanent pH 5.7-5.9, after stream adds alkali liquor 4-5h,
Natural fermentation is to after pH 4.3-4.7, and stream adds alkali liquor when maintaining fermentation liquid perseverance pH 5.7-5.9 to pH no longer to change
For fermentation termination, for optimal fermentation technology.
The preparation of embodiment 4 compound lactobacillus microbial manure:
(1) it is 7% by four lactobacillus plantarum P-8, PB-1, PY-1 and SCI-02 by total inoculum concentration, inoculation ratio
Example is: 1:1:1:2, accesses fermentation medium and ferments；
Fermentation condition is as follows: the initial pH of fermentation tank: be 7.0, and fermentation temperature is 30 DEG C, and permanent pH 5.7-5.9, stream adds
After alkali liquor 4h, after natural fermentation to pH 4.3-4.7, stream adds alkali liquor and maintains fermentation liquid perseverance pH 5.7-5.9 to fermentation liquid
It it is fermentation termination when self pH no longer changes；
Fermentation medium composition is as follows: soybean protein powder 20g, sucrose 40g, citric acid 0.7g, K2HPO41g,
Sodium citrate 8g, tween 80 1g, distilled water 1L, pH 7.0；
(2), after having fermented, compound lactobacillus-fermencucumber liquid is mixed with the sucrose after aseptic process and Semen sojae atricolor powder
After uniformly, packaging cold preservation i.e. obtains compound lactobacillus microbial manure；The addition of sucrose and fermentation liquid mass volume ratio
It is 0.1%；The addition of Semen sojae atricolor powder is 0.1%.
The preparation of embodiment 5 compound lactobacillus microbial manure:
(1) it is 7% by four lactobacillus plantarum P-8, PB-1, PY-1 and SCI-02 by total inoculum concentration, inoculation ratio
Example is: 1:1:1:2, accesses fermentation medium and ferments；
Fermentation condition is as follows: the initial pH of fermentation tank: be 7.0, and fermentation temperature is 30 DEG C, and permanent pH 5.7-5.9, stream adds
After alkali liquor 5h, after natural fermentation to pH 4.3-4.7, stream adds alkali liquor and maintains fermentation liquid perseverance pH 5.7-5.9 to pH not exist
It it is ferment terminal during change；
Fermentation medium composition is as follows: soybean protein powder 40g, sucrose 80g, citric acid 1.0g, K2HPO44g,
Sodium citrate 12g, tween 80 3g, distilled water 1L, pH 7.0；
(2), after having fermented, compound lactobacillus-fermencucumber liquid is mixed with the sucrose after aseptic process and Semen sojae atricolor powder
After uniformly, packaging cold preservation i.e. obtains compound lactobacillus microbial manure；The addition of sucrose and fermentation liquid mass volume ratio
It is 0.1%；The addition of Semen sojae atricolor powder is 0.1%.
The preparation of embodiment 6 compound lactobacillus microbial manure:
(1) it is 7% by four lactobacillus plantarum P-8, PB-1, PY-1 and SCI-02 by total inoculum concentration, inoculation ratio
Example is: 1:1:1:2, accesses fermentation medium and ferments；
Fermentation condition is as follows: the initial pH of fermentation tank: be 7.0, and fermentation temperature is 30 DEG C, and permanent pH 5.7-5.9, stream adds
After alkali liquor 4.5h, after natural fermentation to pH 4.3-4.7, stream adds alkali liquor and maintains fermentation liquid perseverance pH 5.7-5.9 to pH not
It is fermentation termination when change；
Fermentation medium composition is as follows: soybean protein powder 30g, sucrose 60g, citric acid 0.8g, K2HPO42g,
Sodium citrate 10g, tween 80 2g, distilled water 1L, pH 7.0；
(2), after having fermented, compound lactobacillus-fermencucumber liquid is mixed with the sucrose after aseptic process and Semen sojae atricolor powder
After uniformly, packaging cold preservation i.e. obtains compound lactobacillus microbial manure；The addition of sucrose and fermentation liquid mass volume ratio
It is 0.1%；The addition of Semen sojae atricolor powder is 0.1%.
The application of embodiment 7 compound lactobacillus microbial manure
A. embodiment 6 gained compound lactobacillus micro-organism fertilizer is applied to cucumber disease suppression
Test packet: cucumber plant is randomly divided into test group and matched group, often organizes each 9 strains.
Test method: test group Fructus Cucumidis sativi is every 15 days foliage-sprays and root irrigation compound lactobacillus microbial manure one
Secondary.Foliage-spray is according to 150ml/ mu/time (using after being diluted by 100 times of fertilizer), root pouring 1000ml/
Mu/time (using after 100 times of fertilizer is diluted).
Experimental observation: a situation arises and the growing state of plant every the disease of 15d observed and recorded cucumber plant.
Trial: test whole process does not use any pesticide and anti-microbial type material, does not use any fertilizer, test group and
Matched group Fructus Cucumidis sativi administrative situation is identical.
Result of the test:
Compound lactobacillus microbial manure can reduce the probability of cucumber plant disease infection and delay disease to increase the weight of
Process, uses and reduces plant after 15d and catch an illness probability 33.4%, reduce degree of disease 25% after using 30d.Multiple
Close lactic acid bacteria microbe fertilizer and there is the risk reducing cucumber plant introduced disease, cucumber plant is had protection and makees
With；Can delay or alleviate cucumber plant disease and increase the weight of process, cucumber plant is had protective effect.See Fig. 1-3.
B. embodiment 5 gained compound lactobacillus micro-organism fertilizer is applied to Fructus Lycopersici esculenti volume increase
Test packet: Fructus Lycopersici esculenti is randomly divided into test group and matched group, often organizes each 0.5 mu.
Test method: test group Fructus Lycopersici esculenti is every 15 days foliage-sprays and root irrigation compound lactobacillus microbial manure
Once.Foliage-spray is according to 150ml/ mu/time (using after being diluted by 100 times of fertilizer), and root waters
1000ml/ mu/time (using after 100 times of fertilizer is diluted).
Experimental observation: every day observed and recorded tomato plant growing state and yield.
Trial: test whole process does not use any pesticide and anti-microbial type material, test group and the management of matched group Fructus Lycopersici esculenti
Situation is identical.
Result of the test:
Lactic acid bacteria fertilizer can remarkably promote Fructus Lycopersici esculenti root system development, accelerates plant strain growth, improves Fructus Lycopersici esculenti individual plant
Average product, raising 14.34% is produced in test group relatively matched group strain.To growing tomatoes soil and western red after test
The sugar content of Fructus Kaki compares discovery: test group pH6.57, substantially less than matched group (pH6.97), vegetable is raw
Long Optimal pH is 6.5-6.8, uses lactic acid bacteria fertilizer that soil is had the effect that has some improvement.Additionally, use
After lactic acid bacteria fertilizer, the sugariness (sugar content) of Fructus Lycopersici esculenti increases, and mouthfeel is improved.Use lactic acid bacteria fertilizer to western red
The quality of Fructus Kaki has improvement result.See Fig. 4-7.
C. embodiment 4 gained compound lactobacillus micro-organism fertilizer is applied to soil microenvironment and fertilizer efficiency
Improve
Test packet: Fructus Lycopersici esculenti, Fructus Cucumidis sativi are randomly divided into test group and matched group, often organize each 1 mu.
Test method: test group is every 15 days foliage-sprays and root irrigation compound lactobacillus microbial manure once.
Foliage-spray is according to 150ml/ mu/time (using after being diluted by 100 times of fertilizer), root pouring 700ml/
Mu/time (using after 100 times of fertilizer is diluted).
Soil K+adsorption: test early stage and post-collection test group and matched group soil sample, the microorganism in detection soil
Content and fertility index.
Trial: test whole process does not use any pesticide and anti-microbial type material, test group and the management of matched group Fructus Lycopersici esculenti
Situation is identical.
Result of the test:
After growing tomatoes uses compound lactobacillus microbial manure, Soil Microbes finds: in soil
Fungi substantially reduces (reducing 28.57%)；In soil, actinomycetes, bacillus cereus and total number of bacteria increase (divide
Do not increase by 6.88%, 10.70% and 46.06%).Compound lactobacillus microbial manure is conducive to improving in soil to be had
Beneficial microorganism, suppresses soil-borne disease fungus, improves soil microenvironment.
Compound lactobacillus microbial manure can substantially reduce soil with organic matter and (refer to: in soil, carbon containing is organic
Compound) content (reducing 27.31%), available phosphorus contents (reducing 33.67%) and alkali-hydrolyzable nitrogen content (subtract
Few 27.68%).May advantageously facilitate soil with organic matter to decompose and nitrogen phosphorus nutrients release, promote Plant To Nutrient unit
The absorption of element.See Fig. 8-9.
D. embodiment 6 gained compound lactobacillus micro-organism fertilizer is applied to stress resistance of plant test
Test packet: Fructus Lycopersici esculenti and rapeseed plants are randomly divided into test group and matched group, often organize each 1 mu.
Test method: test group is every 15 days foliage-sprays and root irrigation compound lactobacillus microbial manure once.
Foliage-spray is according to 150ml/ mu/time (using after being diluted by 100 times of fertilizer), root pouring 1000ml/
Mu/time (using after 100 times of fertilizer is diluted).
Experimental observation: every day observed and recorded Fructus Lycopersici esculenti and rapeseed plants growing state.
Trial: test whole process does not use any pesticide and anti-microbial type material, does not use any fertilizer, test group and
Matched group administrative situation is identical.
Result of the test:
1) Brassica campestris L test finds: during low temperature in February in winter, owing in the range of 1 meter of south, greenhouse, temperature is relatively low, with
The Brassica campestris L of north plantation, plant strain growth significant difference in greenhouse.In the range of 1 meter of south, matched group greenhouse
The average plant height of Brassica campestris L be 12.1cm, the average plant height of north Brassica campestris L be 14.8cm (both difference
2.7cm, significant difference)；The average plant height of Brassica campestris L in the range of 1 meter of south, test group greenhouse is 14.2cm,
Brassica campestris L average plant height in north is 14.7cm (both differ 0.5cm, and difference is the most notable).Spray compound
Lactic acid bacteria microbe fertilizer can strengthen the Brassica campestris L resistance to low temperature.
2) Fructus Lycopersici esculenti experiment find: during high temperature in August in summer, greenhouse temperature higher than 38 degree time, test block kind
Folium Solani Melongenae sheet is normal, and the phenomenon that is Protected from Heat occurs in the blade of check plot, and blade is rolled up.Illustrate to spray compound lactic acid
Bacteria microorganism fertilizer can strengthen the Fructus Lycopersici esculenti resistance to high temperature.
Interpretation of result: compound lactobacillus microbial manure can remarkably promote promotion tomato plant aerial parts
The growth of (plant height, footpath) and root system development, improve the average single plant yield of Fructus Lycopersici esculenti, test group relatively matched group
Raising 14.34% is produced in strain strain.Compound lactobacillus microbial manure is conducive to improving beneficial microbe in soil, presses down
Soil-borne disease fungus processed, improves soil microenvironment.Compound lactobacillus microbial manure may advantageously facilitate soil
Middle organic matter decomposition and nitrogen phosphorus nutrients release, promote the absorption of Plant To Nutrient element..Additionally, use lactic acid
After bacterial manure material, the resistance of high temperature, low temperature and disease is strengthened by plant, beneficially the healthy growth of plant.
Additionally, the sugariness of Fructus Lycopersici esculenti (sugar content) increases, mouthfeel is improved, and the quality of Fructus Lycopersici esculenti is had improvement result.
See accompanying drawing 4-9.
Conclusion:
4 strains of lactic acid bacteria prepare fermentation liquid through composite fermentation, and viable count of lactobacillus reaches 1.06 × 1010CFU/mL
Above.Mixed fermentation liquid makes compound lactobacillus microbial manure with carrier after mixing homogeneously.
Compound lactobacillus microbial manure can remarkably promote aboveground vegetation part (plant height, footpath) growth and
Root system development, improves crop yield.Compound lactobacillus microbial manure is conducive to improving beneficial microbe in soil,
Suppression soil-borne disease fungus, improves soil microenvironment.Compound lactobacillus microbial manure may advantageously facilitate soil
Organic matter decomposition and nitrogen phosphorus nutrients release in earth, promote the absorption of Plant To Nutrient element..Additionally, use breast
After acid bacterial manure material, the resistance of high temperature, low temperature and disease is strengthened by plant, beneficially the healthy growth of plant.
Additionally, fruit sweetness (sugar content) increases, mouthfeel is improved, and quality is had improvement result.
Claims (7)
1. a compound lactobacillus microbial manure, it is characterised in that the preparation method of described compound lactobacillus microbial manure is such as
Under:
(1) it is that 7% access fermentation medium is carried out by four lactobacillus plantarum P-8, PB-1, PY-1 and SCI-02 by total inoculum concentration
Fermentation, fermentation condition is as follows: the initial pH of fermentation tank is 7.0, and fermentation temperature is 30 DEG C, and permanent pH 5.7-5.9, stream adds alkali liquor 4-5h
After, after natural fermentation to pH 4.3-4.7, stream adds alkali liquor and maintains the fermentation liquid perseverance pH 5.7-5.9 to fermentation liquid self pH when not changing to be
Fermentation termination；
(2) after having fermented, compound lactobacillus-fermencucumber liquid is mixed homogeneously with the sucrose after aseptic process and Semen sojae atricolor powder i.e. obtain compound
Lactic acid bacteria microbe fertilizer.
2. a kind of compound lactobacillus microbial manure as claimed in claim 1, it is characterised in that described Lactobacillus plantarum SCI-02
It is specially Lactobacillus plantarum (Lactobacillus plantarum) SCI-02, deposit number CGMCC No.11931.
3. a kind of compound lactobacillus microbial manure as claimed in claim 1, it is characterised in that described Lactobacillus plantarum P-8,
The inoculative proportion of PB-1, PY-1 and SCI-02 is: 1:1:1:2.
4. a kind of compound lactobacillus microbial manure as claimed in claim 1, it is characterised in that described fermentation medium forms
As follows: soybean protein powder 20-40g, sucrose 40-80g, citric acid 0.7-1.0g, K2HPO41-4g, sodium citrate 8-12g, tell
Temperature-80 1-3g, distilled water 1L, pH 7.0.
5. compound lactobacillus microbial manure as claimed in claim 1 a kind of, it is characterised in that the addition of described sucrose with
Fermentation liquid mass volume ratio is 0.1%；The addition of described Semen sojae atricolor powder and fermentation liquid mass volume ratio are 0.1%.
6. compound lactobacillus microbial manure application during proportion of crop planting described in claim 1.
7. compound lactobacillus microbial manure application during proportion of crop planting as claimed in claim 6, it is characterised in that
Every 15-30 days, after 100 times of fertilizer is diluted, foliage-spray according to 100-200ml/ mu/time use, root pouring according to
500-1000ml/ mu/time use.
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Denomination of invention: A Compound Lactic Acid Bacteria Microbial Fertilizer and Its Preparation and Application Effective date of registration: 20230515 Granted publication date: 20190510 Pledgee: Bank of China Limited Hohhot Xincheng sub branch Pledgor: Inner Mongolia Zhongfeng Biotechnology Co.,Ltd. Registration number: Y2023150000072 |