CN104488400A - Method for promoting germination of Yulangsan seeds - Google Patents
Method for promoting germination of Yulangsan seeds Download PDFInfo
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- CN104488400A CN104488400A CN201410714685.6A CN201410714685A CN104488400A CN 104488400 A CN104488400 A CN 104488400A CN 201410714685 A CN201410714685 A CN 201410714685A CN 104488400 A CN104488400 A CN 104488400A
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Abstract
The invention discloses a method for promoting germination of Yulangsan seeds. The method comprises the following steps: completely soaking pretreated Yulangsan seeds for 6-36 hours in gibberellin solution of which the concentration is 50mg/L to 400mg/L; culturing the soaked Yulangsan seeds at constant temperature. The method disclosed by the invention is used for remarkably increasing the germination rate of the Yulangsan seeds and has the advantages of rapid germination and orderly and strong sprouts; meanwhile, the method disclosed by the invention is simple and convenient to operate, low in cost, high in practicability, free of pollution, easy to popularize, capable of laying a firm foundation for large-scale artificial cultivation of Yulangsan, and good in economic benefits and social benefits.
Description
Technical field
The invention belongs to plant seed germination technical field, particularly a kind of method promoting beautiful youth's umbrella seed germination.
Background technology
Beautiful youth's umbrella is that Papilionaceae plant dredges leaf precipice beans [Millettia pulchra Kurz var.laxior (Dunn) Z.Wei], have another name called laxleaf millettia root, first appeared in " Guangxi Chinese medicinal herbal ", it is the local medicinal material that " Guangxi traditional Chinese medicine standard " (nineteen ninety version) is recorded.Beautiful youth's umbrella is Guangxi Zhuang, Yao nationality's medicinal herbs most in use, is used as medicine with dried root, and property is put down, and taste is sweet, micro-pungent, returns Liver Channel; There is eliminating stasis to subdue swelling, effect that pain relieving is allayed excitement, be used for the treatment of infantile malnutrition, postpartum and after being ill weakness, treating swelling and pain by traumatic injury, fracture, Fengshi Guanjie swell and ache, the disease such as hemiplegia, mental retardation of children and senile dementia, sore swollen toxin, rubella are itched, indigestion.Research in recent years shows, beautiful youth's umbrella also has tonifying Qi, enriches blood, resisting stress, anti anoxia, the effect such as raising immunity of organisms and step-down.Beautiful youth's umbrella is distributed in the provinces and regions such as China Guangxi, Hainan, Yunnan, Guizhou, mainly originate in the Liuzhou in Guangxi, Lingui, reach the clouds, Nanning, Tiane, Longan, Wuming, Liujiang, Wuxuan, the ground such as Cenxi.
The production of beautiful youth's umbrella is mainly by wild resource, and its Regional Distribution is scattered and reserves rare.Along with the exploitation of series of products such as " the swelling and pain relieving essences " that be primary raw material with beautiful youth's umbrella, its market demand rises year by year, and wild resource suffers excessively to excavate, and is on the verge of exhaustion.Beautiful youth's umbrella mainly relies on seed to breed, but grain weight is very rare, and sprouting is slow in its natural state, germination rate is low, it is irregular to emerge, so that is difficult to realize commerial growing on producing.Prior art is not for the report promoting beautiful youth's umbrella seed germination.
Summary of the invention
The object of the present invention is to provide a kind of method promoting beautiful youth's umbrella seed germination, to solve, beautiful youth's umbrella seed is sprouted slowly in its natural state, germination rate is low, irregular problem of emerging, for extensive artificial cultivation provides a large amount of good seeds, with the needs in satisfied production.
Technical scheme provided by the invention is:
Promote a method for beautiful youth's umbrella seed germination, comprise the following steps:
To be soaked in completely in Gibberellins solution through pretreated beautiful youth's umbrella seed, wherein said Gibberellins solution concentration is 50 ~ 400mg/L, and soak time is 6 ~ 36h;
Beautiful youth's umbrella seed through seed soaking is carried out constant temperature culture.
Preferably, in the method for described promotion beautiful youth's umbrella seed germination, described pretreatment comprises:
(1) gather seed: annual November gathers ripe beautiful youth's umbrella fruit, take out seed, clean, select not damaged, full seed, seed that is homogeneous, that do not carry disease worm's ovum are for subsequent use;
(2) seed disinfection: the seed that described step (1) is selected is placed in mass fraction be 3% liquor natrii hypochloritis soak 20min, then be placed in mass fraction be 10% trisodium phosphate solution soak 30min, distilled water flushing 5 times;
(3) ultrasonic process: the seed that described step (2) was disinfected is put into ultrasonic cleaner ultrasonic process 20min.
Preferably, in the method for described promotion beautiful youth's umbrella seed germination, described constant temperature culture comprises: be placed on two-layer fully moistening sprouting paper by the beautiful youth's umbrella seed through seed soaking, then described sprouting paper is put in clear glass culture dish, and cover lid, be finally put in constant incubator by described clear glass culture dish, described constant incubator temperature is 18 ~ 35 DEG C, described constant incubator humidity is 70 ~ 80%, and the time is 15 ~ 30 days.
Preferably, in the method for described promotion beautiful youth's umbrella seed germination, described gibberellin concentration is 100mg/L, and seed soaking time is 24h.
Preferably, in the method for described promotion beautiful youth's umbrella seed germination, described constant incubator temperature is 28 DEG C.
Preferably, in the method for described promotion beautiful youth's umbrella seed germination, described liquor natrii hypochloritis and described trisodium phosphate solution all will fully stir to get rid of its air entrapment.
Preferably, in the method for described promotion beautiful youth's umbrella seed germination, the frequency of described ultrasonic cleaner is 40kHz.
Preferably, in the method for described promotion beautiful youth's umbrella seed germination, described constant incubator is the constant incubator that 12h illumination/12h dark replaces.
The present invention adopts gibberellin seed soaking, beautiful youth's umbrella seed cell can be induced to divide and differentiation and promote growth and the seed germination of seed embryo, improve content and the vigor of α-amylase in seed, increase Peroxidase activity, promote seed breaking dormancy, improve seed germination rate, greatly improve the germination rate of beautiful youth's umbrella seed, and the seed of present treatment process and process is without any side effects, to seed contactee also without dangerous, environmental pollution can not be caused, have concurrently easy and simple to handle simultaneously, the advantage that cost is low, there is stronger practicality and promotional value, and utilize the method for ultrasonic process and constant temperature culture, the germination rate of further raising beautiful youth's umbrella seed.
The invention belongs to first to the achievement in research promoting beautiful youth's umbrella seed germination technology, significantly improve the germination rate of beautiful youth's umbrella seed, and it is fast to have sprouting, to sprout neat, healthy and strong advantage, simultaneously the inventive method is easy and simple to handle, and cost is low, practical, pollution-free and easily promote, for the extensive artificial cultivation establish a firm foundation of beautiful youth's umbrella, there is good economic benefit and social benefit.
Embodiment
Below in conjunction with specific embodiment, the invention will be further described, can implement according to this with reference to specification word to make those skilled in the art.
Compound concentration is the step of 50-400mg/L Gibberellins solution: first from the refrigerator of-20 DEG C, take out gibberellin powder, accurately take 50mg respectively, 100mg, 200mg, 300mg, 400mg pours in 5 corresponding test tubes, then the ethanol dissolving 20-30min that concentration is 95% is respectively added, after gibberellin fully dissolves, slowly pour into and fill in 5 corresponding beakers of 600-800mL distilled water, predominate while stir, gibberellin is avoided to produce recrystallization, finally use distilled water constant volume in 5 corresponding 1000mL volumetric flasks, put upside down and shake up, namely join concentration is 50mg/L, 100mg/L, 200mg/L, 300mg/L, the Gibberellins solution of 400mg/L.
(1) different seed soaking time is on the impact of beautiful youth's umbrella seed germination
Respectively beautiful youth's umbrella seed being placed in concentration is after the Gibberellins solution of 100mg/L soaks 6h, 12h, 24h and 36h, pouring out seed is directly put on culture dish interior sprouting paper, add a cover, then place in the constant incubator of 25 DEG C and cultivate 30d, observe the impact (table 1) of different seed soaking time on beautiful youth's umbrella seed germination.
The different seed soaking time of table 1 is on the impact of beautiful youth's umbrella seed germination
Result shows, seed soaking time is comparatively large on the impact of beautiful youth's umbrella seed germination, and statistical analysis shows the germination rate significant difference between different seed soaking time process, in conjunction with the robustness sprouting regularity and bud, seed soaking 24h best results, during constant temperature culture 30d, germination rate is up to 96%.
(2) impact of variable concentrations Gibberellins solution seed soaking on beautiful youth's umbrella seed germination
Respectively beautiful youth's umbrella seed being placed in CK and concentration is that the Gibberellins solution of 50mg/L, 100mg/L, 150mg/L, 200mg/L, 400mg/L soaks 24h, then place in the constant incubator of 25 DEG C and cultivate 30d, observe the impact (table 2) of variable concentrations gibberellin seed soaking on beautiful youth's umbrella seed germination.
The impact of table 2 variable concentrations Gibberellins solution seed soaking on beautiful youth's umbrella seed germination
Result shows, gibberellin seed soaking has the sprouting of beautiful youth's umbrella seed extremely significantly to be affected, and the germination rate difference between statistical analysis display variable concentrations gibberellin seed soaking and CK is extremely remarkable.In conjunction with the robustness sprouting regularity and bud, be that the Gibberellins solution seed soaking of 100mg/L and 150mg/L is excellent with concentration, during constant temperature culture 30d, both germination rates are all up to more than 91%; The treatment effect with concentration being wherein the Gibberellins solution of 100mg/L is best, and during constant temperature culture 30d, germination rate is up to 96%.
(3) different cultivation temperature is on the impact of beautiful youth's umbrella seed germination
Beautiful youth's umbrella seed being placed in concentration is after the Gibberellins solution of 100mg/L soaks 24h, be put in the constant incubator of 18 DEG C, 22 DEG C, 25 DEG C, 28 DEG C, 30 DEG C, 35 DEG C respectively, cultivate 30d, observe the impact (table 3) of different cultivation temperature on beautiful youth's umbrella seed germination.
The different cultivation temperature of table 3 is on the impact of beautiful youth's umbrella seed germination
Result shows, the sprouting of different cultivation temperature on beautiful youth's umbrella seed has remarkable impact, and statistical analysis shows the germination rate significant difference between different cultivation temperature process.When cultivation temperature is low to moderate 18 DEG C, when cultivating 20d, beautiful youth's umbrella seed is not also sprouted; When cultivating 30d, the germination rate of beautiful youth's umbrella seed just reaches 31%.When cultivation temperature is up to 35 DEG C, there is deliquescing, send out rotten and Signs of Mould in beautiful youth's umbrella seed in incubation, and explanation should not be cultivated at 35 DEG C and above temperature.The robustness of comprehensive sprouting regularity and bud, 25 DEG C and 28 DEG C is the preference temperature of beautiful youth's umbrella seed germination, and when cultivating 30d, both germination rates are all up to more than 96%; Wherein best with the treatment effect of 28 DEG C, when cultivating 30d, germination rate is up to 98%.
In sum, promote in the method for beautiful youth's umbrella seed germination, preferred plan is that beautiful youth's umbrella seed to be placed in concentration be that the Gibberellins solution of 100mg/L soaks 24h, then cultivates in the constant incubator of 28 DEG C.Beautiful youth's umbrella seed germination that the program obtains is fast, and germination rate is high, teethes neat, healthy and strong.
Although embodiment of the present invention are open as above, but it is not restricted to listed in specification and embodiment utilization, it can be applied to various applicable the field of the invention completely, for those skilled in the art, can easily realize other amendment, therefore do not deviating under the universal that claim and equivalency range limit, the present invention is not limited to specific details and illustrates here and the embodiment described.
Claims (8)
1. promote a method for beautiful youth's umbrella seed germination, it is characterized in that, comprise the following steps:
To be soaked in completely in Gibberellins solution through pretreated beautiful youth's umbrella seed, wherein said Gibberellins solution concentration is 50 ~ 400mg/L, and soak time is 6 ~ 36h;
Beautiful youth's umbrella seed through seed soaking is carried out constant temperature culture.
2. the method promoting beautiful youth's umbrella seed germination as claimed in claim 1, it is characterized in that, described pretreatment comprises:
(1) gather seed: annual November gathers ripe beautiful youth's umbrella fruit, take out seed, clean, select not damaged, full seed, seed that is homogeneous, that do not carry disease worm's ovum are for subsequent use;
(2) seed disinfection: the seed that described step (1) is selected is placed in mass fraction be 3% liquor natrii hypochloritis soak 20min, then be placed in mass fraction be 10% trisodium phosphate solution soak 30min, distilled water flushing 5 times;
(3) ultrasonic process: the seed that described step (2) was disinfected is put into ultrasonic cleaner ultrasonic process 20min.
3. the method promoting beautiful youth's umbrella seed germination as claimed in claim 1, it is characterized in that, described constant temperature culture comprises: be placed on two-layer fully moistening sprouting paper by the beautiful youth's umbrella seed through seed soaking, then described sprouting paper is put in clear glass culture dish, and cover lid, be finally put in constant incubator by described clear glass culture dish, described constant incubator temperature is 18 ~ 35 DEG C, described constant incubator humidity is 70 ~ 80%, and the time is 15 ~ 30 days.
4. the method promoting beautiful youth's umbrella seed germination as claimed in claim 1, it is characterized in that, described gibberellin concentration is 100mg/L, and seed soaking time is 24h.
5. the method promoting beautiful youth's umbrella seed germination as claimed in claim 3, it is characterized in that, described constant incubator temperature is 28 DEG C.
6. the method promoting beautiful youth's umbrella seed germination as claimed in claim 2, it is characterized in that, described liquor natrii hypochloritis and described trisodium phosphate solution all will fully stir to get rid of its air entrapment.
7. the method promoting beautiful youth's umbrella seed germination as claimed in claim 2, it is characterized in that, the frequency of described ultrasonic cleaner is 40kHz.
8. the method promoting beautiful youth's umbrella seed germination as claimed in claim 3, is characterized in that, described constant incubator is the constant incubator that 12h illumination/12h dark replaces.
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| CN201410714685.6A CN104488400B (en) | 2014-11-28 | 2014-11-28 | A kind of method promoting beautiful youth's umbrella seed germination |
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| CN201410714685.6A CN104488400B (en) | 2014-11-28 | 2014-11-28 | A kind of method promoting beautiful youth's umbrella seed germination |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104855290A (en) * | 2015-05-27 | 2015-08-26 | 广西壮族自治区药用植物园 | Yulangsan tissue culturing method |
| CN105917794A (en) * | 2016-05-10 | 2016-09-07 | 江苏省林业科学研究院 | Seed treatment method for sowing and seedling raising of Dalbergia hupeana Hance |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1187933A (en) * | 1997-01-13 | 1998-07-22 | 丁庆 | Cultivation method for crassula perforate, cotyledon malacophylla, monochoria vaginalis fordia cauliflora |
| CN103650697A (en) * | 2012-09-21 | 2014-03-26 | 张勇 | Method for promoting cynomorium songaricum seeds to germinate |
| CN104094762A (en) * | 2014-07-18 | 2014-10-15 | 安顺雄丰药业发展有限公司 | Quick cultivation method of Paris polyphylla seedlings |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1187933A (en) * | 1997-01-13 | 1998-07-22 | 丁庆 | Cultivation method for crassula perforate, cotyledon malacophylla, monochoria vaginalis fordia cauliflora |
| CN103650697A (en) * | 2012-09-21 | 2014-03-26 | 张勇 | Method for promoting cynomorium songaricum seeds to germinate |
| CN104094762A (en) * | 2014-07-18 | 2014-10-15 | 安顺雄丰药业发展有限公司 | Quick cultivation method of Paris polyphylla seedlings |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104855290A (en) * | 2015-05-27 | 2015-08-26 | 广西壮族自治区药用植物园 | Yulangsan tissue culturing method |
| CN104855290B (en) * | 2015-05-27 | 2017-06-16 | 广西壮族自治区药用植物园 | The method for tissue culture of beautiful youth's umbrella |
| CN105917794A (en) * | 2016-05-10 | 2016-09-07 | 江苏省林业科学研究院 | Seed treatment method for sowing and seedling raising of Dalbergia hupeana Hance |
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| CN104488400B (en) | 2016-02-17 |
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