CN105724298A - Method for factory puffer fish fry cultivation - Google Patents
Method for factory puffer fish fry cultivation Download PDFInfo
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- CN105724298A CN105724298A CN201610209258.1A CN201610209258A CN105724298A CN 105724298 A CN105724298 A CN 105724298A CN 201610209258 A CN201610209258 A CN 201610209258A CN 105724298 A CN105724298 A CN 105724298A
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Farming Of Fish And Shellfish (AREA)
Abstract
The invention relates to a method for factory puffer fish fry cultivation. The method comprises the following production steps: parent fish cultivation and screening, artificial spawning, artificial insemination, artificial incubation, factory fish fry cultivation, outdoor large-size fingerling cultivation, fish fry transportation and the like. The method is characterized in that female fishes with an age of over three years and a weight of more than 750 grams and male fishes with an age of over two years and a weight of more than 45 grams serving as puffer fishes are taken as backup parents; a simulated natural ecological cultivation mode is adopted. The artificial spawning is performed by adopting combined leuteinizing hormone releasing hormone analogue and carp pituitary gland injection. The incubation of fertilized eggs is performed by adopting incubating barrel incubation in flowing water or ovum sticking plate incubation in still water. According to the method provided by the invention, the production functions of the puffer fishes are improved through scientifically controlling the breeding environment of the puffer fishes, so that a large amount of ovum and sperms is produced; in the cultivation and growth environment of fries in a simulated natural environment state, the survival rates and the growth speed of the fries are increased.
Description
Technical field
The present invention relates to a kind of river batch production offspring seed cultivation method, belong to technical field of aquaculture.
Background technology
River just belongs to treasure in fish since ancient times, is delicious food in banquet, enjoys people to favor.East genus has more than 20 kind, and what be suitable for artificial cultivation has four or five kinds, and wherein sea-farming has two kinds, only two kinds of freshwater aquiculture, i.e. Puffer fish and fugu ocellatus.Japan's sixties have carried out the artificial breeding research in red fin east, just succeed.70 to the nineties, achieve certain achievement in the artificial breeding technology of river fish, and reach the level commercially produced.The artificial propagation research of China ChangJiang River fresh water river fish starts from late nineteen seventies, and the eighties mainly nursery and cultural technique to sea water river fish has been studied.The nineties just starts to have carried out widely studied to breeding of freshwater aquiculture kind Puffer fish, but nursery parent still by fishing for from the Changjiang river, it is impossible to carries out full artificial breeding control.
River has anadromy migration habit, sexually matured river of annual the end of spring and the beginning of summer, trace back river and on, group swims that to lay eggs into river, produce the phase of incubating in the Changjiang river be the 4-6 month, and May is the phase of abounding with, general 140,000-30 ten thousand of every tail female river fish brood amount.Past attempts is distributed widely in China East Sea, the yellow Bohai Sea and In Middle And Lower Reaches of Changjiang River, it is important aquatic resources, but in recent years owing to environmental pollution, transition are fished for and the reasons such as migration access denial, Puffer fish natural resources amount is made sharply to decline, the middle and lower reach of Yangtze River is the major production areas of river fish, and annual production in 1954 reaches about 1000t, and current production rate is only up to tens tons.The market demand but constantly rises, depend alone fish for natural Puffer fish can not meet people consumption demand, the whole artificial propagation technical research carrying out river fish is extremely urgent, be also become solve imbalance between supply and demand only way.Conventional research shows, without anadromous migration, the gonad in river can not be fully grown.So under Artificial Control regulates, natural ecological environment in simulation migration process, especially make the stimulation of salinity, reaching maturity through gonad can be urged, there is critically important theory and realistic meaning undoubtedly.Cultivate river seed at present and generally all adopt pond rearing.Due to pond rearing subject to pest and disease damage, agreeable to the taste bait difficulty meet, Seedling is easily mutually residual, adds that early stage nursery stage temperature Change is frequent, time have cold wave to go down south problems such as causing large quantities of fry dead, domestic production survival rate of seedling is generally 30%, and the highest survival rate of seedling is about 50%.
Summary of the invention
In order to meet the artificial industrial seedling rearing in river, the present invention proposes a kind of river batch production offspring seed cultivation method.
The production technology step of the present invention includes: the production stage compositions such as parent fish rearing, parent fish screening, artificial induced spawning, artificial insemination, artificial incubation, factory fry cultivation, outdoor large size fingerling cultivation and seed transport.
Parent fish rearing: adopting the measure such as circulating filtration and vortex air pump oxygen supplement, make Dissolved Oxygen in Water at more than 5mg/L, salinity is between 10-15 ‰, and water temperature is between 18-25 DEG C.Soil pond stocking rate is 0.2-0.3kg/m2, cement pit lofting amount is 1.5-2kg/m2, female-male proportion 1.5:1.
Parent fish is screened: more than raun 3 age, body weight more than 750 grams, abdominal part expands, and cloacal aperture is micro-red and lax；More than milter 2 age, body weight more than 450 grams, abdominal part is slightly loose soft, presses fish body abdominal part to have milky seminal fluid to flow out with have gentle hands.
Artificial induced spawning: injecting hasten parturition LRH-A hormone and Medulla Cyprinus carpio hypophysis, LRH-A hormone dosage of hastening parturition is 8 μ g, Medulla Cyprinus carpio hypophysis 1-2/tail, point biphasic injection, first time injects the 1/4 of accumulated dose, injects surplus for the second time.
Artificial insemination: dry parent fish body surface moisture, extracts in dry smooth container by fish roe, squeezes on ovum by milter seminal fluid rapidly simultaneously, is gently mixed uniformly with banister brush, makes essence ovum be sufficiently mixed, then adds clear water stirring, after standing 2-3 minute, changes water 2-3 time, clear to water.Whole operating process should complete in 30min.
Artificial incubation: the Seedling that Lentic hatching and Lotic hatching are hatched moves in seedling box at any time, passes through siphonage Inlet and outlet water oxygenation with Chi Xiatang to be entered with small rubber hose.
Food organisms are cultivated: different cultivation stage provides different bait, and after hatching, the starts to throw something and feed egg yolk two days for 3-4 days, and one piece of egg yolk feeds 10 5 ten thousand tails, every day 5 times, and within the 5th day, disposable chlorella 10 liters of splashing in water is (containing about 5.6 × 109Frustule), start wheel animalcule of throwing something and feeding, throw something and feed every day 5 times, in seedling water, wheel animalcule density is maintained at 5-10/ml.Starting to add throwing artemia nauplii after 10th day by pond Midst density 1-2/ml, the throws something and feeds blood worm for 20-25 days, day feeding volume be about the 20-50% of Seedling body gross weight, every day throws something and feeds 4-3 time, throws something and feeds Limnodrilus hoffmeisteri and Anguillar japonica bait for 35-40 days, and daily ration of feeding is the 30-60% of Seedling TBW, throws something and feeds every day 3 times.
Breeding density: first adopt seedling box, density is 50000 tails/m3Cultivating 3 days under miniflow water and venting condition, within the 4th day, proceed to Small cement pond, density reduces to 3000/m3, cultivate 4 days, the 8th day starts rare to 1500 tails/m3, density was down to again in the 12nd day 750 tails/m3, the 15th day starts to proceed to flood mud sump, and now density is 160-200 tail/m3, until going out the pool (about 40 days) this fry total length to reach 2.5-3cm.
Cultivating and managing: change water: miniflow ability of swimming cultivated day quantity of exchanged water 500-600%, 4-15 light quantity of exchanged water 20-15% in first 3 days, and the later stage cultivates (the 15-40 days) and moves into 270m2Great Chi (depth of water is about 45cm), day quantity of exchanged water 10-15% (clearance-type running water technology).Inflation: early stage cultivates at 24 hours every day, the later stage cultivates at 20 hours every day.Water temperature: early stage cultivates 17-26 DEG C, the later stage cultivates 18-32 DEG C, dissolved oxygen: 4-8mg/L.PH:6.7-6.8.Daytime light intensity 500-10000lux.
The invention has the beneficial effects as follows: the present invention, by the scientific breeding environment controlling river, improves the production function in river, thus a large amount of output ovum and sperm；The cultivation of seed, growing environment under simulating natural environment state, substantially increase survival rate and the speed of growth of seed, finally provides more accurate and scientific foundation for batch production, extensive artificial propagation river.
Detailed description of the invention
Below technical scheme is further described, but technical solution of the present invention so, every modified or equivalent replaces by limitation, without deviating from the spirit and scope of technical solution of the present invention, all should be encompassed in protection scope of the present invention.
A kind of river batch production offspring seed cultivation method, including parent fish rearing, parent fish screening, artificial induced spawning, artificial insemination, artificial incubation, factory fry is cultivated, outdoor large size fingerling is cultivated and seed transport production step.
During described parent fish rearing, the native pool area 2000-6000m cultivated2, the depth of water is at 1.2-1.5m；Indoor temperature control cement pit area is 50-80m2, the depth of water adopts circulating filtration and vortex air pump oxygen supplement at 0.6-0.8m, Tu Chi, it is ensured that Dissolved Oxygen in Water is at more than 5mg/L, and salinity is between 10-15 ‰, and water temperature is between 18-25 DEG C, and soil pond lofting amount is 0.2-0.3kg/m2, cement pit lofting amount is 1.5-2kg/m2, female-male proportion 1.5:1, with fresh food, if fresh fish, Limnodrilus hoffmeisteri, Bellamya quadrata, shrimps etc. are main, eel compound feed of arranging in pairs or groups, adds appropriate vitamin and nutritive salt promotes river gonad development, feeding volume is the 2-3% of river body weight, sooner or later respectively throws something and feeds once, adopts food platform to throw something and feed.
During the screening of described parent fish, select that physique is strong, desalination fully, the parent fish parent fish in support of gonadal maturation, more than raun 3 age, body weight more than 750 grams, abdominal part expands, and cloacal aperture is micro-red and lax；More than milter 2 age, body weight more than 450 grams, abdominal part is slightly loose soft, presses fish body abdominal part to have milky seminal fluid to flow out with have gentle hands.
In described artificial induced spawning, different according to gonad development degree, the oxytocic hormone of injection various dose, injecting hasten parturition LRH-A hormone and Medulla Cyprinus carpio hypophysis, LRH-A hormone dosage of hastening parturition is 8 μ g, Medulla Cyprinus carpio hypophysis 1-2/tail, divide biphasic injection, first time injects the 1/4 of accumulated dose, and second time injects surplus, adopts muscle of back injection or intrathecal.
During artificial insemination, dry parent fish body surface moisture, fish roe is extracted in dry smooth container, rapidly milter seminal fluid is squeezed on ovum simultaneously, be gently mixed uniformly with banister brush, essence ovum is made to be sufficiently mixed, add clear water stirring again, after standing 2-3 minute, change water 2-3 time, clear to water, whole operating process completes in 30min.
During described artificial incubation, incubating oosperm mode mainly adopts hatching barrel Lotic hatching and viscous ovum plate Lentic hatching, hatching barrel Lotic hatching: be sewn into cone with nylon mesh tulle, mesh size is 250 μm, diameter 60cm, height 80cm, bottom, in funnel-form, is placed in cement pit, bottom inflatable, Lotic hatching, hatching density is 1500-2000 grain/L；Viscous ovum plate Lentic hatching: make the framework of long 80-100cm, wide 40-60cm with batten, it is sewn on framework makes viscous ovum plate with cotton gauze or nylon bolting silk that mesh size is 250 μm, Lentic hatching is put into by sticking the viscous ovum plate having germ cell, hatching density: every square centimeter of uniform adhesion germ cell 3-4 grain on viscous ovum plate, in whole hatching process, temperature rests in 18-22 DEG C, and illumination controls at 600-1000lx, and oxygen in water is more than 6mg/L.
The Seedling that hatching barrel Lotic hatching and viscous ovum plate Lentic hatching are hatched moves in seedling box at any time, passes through siphonage Inlet and outlet water oxygenation with Chi Xiatang to be entered with small rubber hose.
The temperature kept relative stability, sufficient dissolved oxygen, good water quality, flow circuit water body be the key of success or failure: 1. hasten parturition, incubation temperature, it is typically in 18 DEG C-22 DEG C to be advisable, within the scope of this, the rising hatching speed along with temperature increases, and minimum temperature and maximum temperature seedling-growing time can differ 2-3d.The advantage of low temperature is: embryo's nurture time is long, stalwartness of emerging, and sperm becomes live time long, and rate of fertilization is beneficial to, and the low gonad development of temperature unboiled water slow, easy is mould.Temperature height gonad development is fast, it is fast to emerge.Temperature is hastened parturition lower than 18 DEG C of impacts, affects river embryonic development, and higher than 22 DEG C of impact fertilizations, the too high meeting of temperature makes rate of fertilization decline, the overdone even all pseudogamies of ovum.2. dissolved oxygen in water is advisable with 6-8mg/L, and river fish is high a lot of to the more general fish of requirement of oxygen.Dissolved oxygen amount is high, the more aobvious active health of parent fish, and hatching is emerged also fast.3. the water body of salubrious circulation, can increase dissolved oxygen, can activate gonad development and lay eggs.Substantially the spawning ground in river is all the great Hui pool in the Changjiang river, and this is also that parent fish breeds the simulation laid eggs under field conditions (factors), makes its Production conditions reflection, copulation of oestrusing.The water quality of cleaning is important measures of anti-oviparity fish molds and pest and disease damage.
When factory fry is cultivated, river is cultivated under water temperature 18-32 DEG C of condition, death occurs lower than 10 DEG C or higher than 34 DEG C.Optimum temperature is 22-28 DEG C, and along with the rising of water temperature within the scope of this, the mobility of fry strengthens, increase of ingesting, and the speed of growth is accelerated.Illumination can improve water temperature, is beneficial to sterilization, prevents the diseases such as fish molds, promote the absorption to nutrition of the fish body, strengthens the body constitution of fish.The nursery of different time needs different water temperatures and illumination, takes the low illumination of early stage, the principle of later stage height illumination, controls water temperature and illumination with two-layer sunshade net；Putting Seedling the last week, improve the Biomass of pond zooplankton by using bio-fertilizer, fry enters before pond with the sodium chloride solution sterilization 10-15min of 2%-3%, and early stage cultivates density at 1000-2000 tail/m3, the later stage cultivates density at 150-250 tail/m3, growth promoter and the survival rate of Larva and fry are had a great impact by culture density.River different developmental phases, need the agreeable to the taste bait of different size, and the later stage cultivate food ration increase quickly, when growing to 25cm, the feeding volume of one day Limnodrilus hoffmeisteri is up to the 60% of Seedling body gross weight, even higher, once Seedling body constitution degradation is not gone up in bait supply, even mutually residual.Adopt chlorella organic debris to cultivate wheel animalcule, blood worm duty is fed, transition period ensures that bait is sufficient, agreeable to the taste, when the later stage cultivates first in great Chi, fill band chlorella, wheel animalcule, blood worm 10 centimeters of old water deep, then, add new water to 40 centimeters, Semen Glycines soybean milk 20 liter/day of splashing, splash three, after five days, water is light green color, defining microorganisms in water chain, have certain self-purification capacity, then band water is transplanted seedlings again；Entering the later stage cultivates, and accomplishes there is complementarity living bait in pond, additionally notes also the conversion time of bait, the variation of bait, in case vitamin, the shortage of the nutrition such as aminoacid.The fish poor growth that bait is not enough, dysplasia is easy to get body.Seedling is cultivated the dissolved oxygen in water needed and is kept 6-8mg/L, in high density factory training seedling water, keep water cleaning, control Excreta, bait residue, the increase controlling nonionic state nitrogen and content of nitrite is to ensure that Seedling healthy growth, it is prevented that the key factor of morbidity.General ammonia-nitrogen content is at 0-1mg/L, and during later stage nursery, can disposable splash lime water 20-40ppm on the every ten, to purify water, improves pH value, reduces ammonia-nitrogen content, it is therefore prevented that disease death.
When outdoor large size fingerling is cultivated, cultivate fingerling, breeding density 10-30 tail/m with cement pit2, stocking size is 30-100mm, and the culture-cycle is 60-150 days, and forming specification is 150-250mm.
In seed transport, taking the water shipment seed in harvesting waters, dissolved oxygen is not less than 6mg/L in transit, and transport water temperature changes less than 5 DEG C, adopts live fish car transport seed, and haulage time was less than 10 hours, and fish and water ratio is not more than 1:80.
Food organisms are cultivated: different cultivation stage provides different bait, and after hatching, the starts to throw something and feed egg yolk two days for 3-4 days, and one piece of egg yolk feeds 10 5 ten thousand tails, every day 5 times, and within the 5th day, disposable chlorella 10 liters of splashing in water is (containing about 5.6 × 109Frustule), start wheel animalcule of throwing something and feeding, throw something and feed every day 5 times, in seedling water, wheel animalcule density is maintained at 5-10/ml.Starting to add throwing artemia nauplii after 10th day by pond Midst density 1-2/ml, the throws something and feeds blood worm for 20-25 days, day feeding volume be about the 20-50% of Seedling body gross weight, every day throws something and feeds 4-3 time, throws something and feeds Limnodrilus hoffmeisteri and Anguillar japonica bait for 35-40 days, and daily ration of feeding is the 30-60% of Seedling TBW, throws something and feeds every day 3 times.
Breeding density: first adopt seedling box, density is 50000 tails/m3Cultivating 3 days under miniflow water and venting condition, within the 4th day, proceed to Small cement pond, density reduces to 3000/m3, cultivate 4 days, the 8th day starts rare to 1500 tails/m3, density was down to again in the 12nd day 750 tails/m3, the 15th day starts to proceed to flood mud sump, and now density is 160-200 tail/m3, until going out the pool (about 40 days) this fry total length to reach 2.5-3cm.
Cultivating and managing: change water: miniflow ability of swimming cultivated day quantity of exchanged water 500-600%, 4-15 light quantity of exchanged water 20-15% in first 3 days, and the later stage cultivates (the 15-40 days) and moves into 270m2Great Chi (depth of water is about 45cm), day quantity of exchanged water 10-15% (clearance-type running water technology).Inflation: early stage cultivates at 24 hours every day, the later stage cultivates at 20 hours every day.Water temperature: early stage cultivates 17-26 DEG C, the later stage cultivates 18-32 DEG C, dissolved oxygen: 4-8mg/L.PH:6.7-6.8.Daytime light intensity 500-10000lux.
The ultimate principle of the present invention, principal character and advantages of the present invention have more than been shown and described.Skilled person will appreciate that of the industry; the present invention is not restricted to the described embodiments; simply principles of the invention described in above-described embodiment and description; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements both fall within the claimed present invention.Claimed scope is defined by appending claims and equivalent thereof.
Claims (10)
1. a river batch production offspring seed cultivation method, it is characterised in that: include parent fish rearing, parent fish screening, artificial induced spawning, artificial insemination, artificial incubation, factory fry is cultivated, outdoor large size fingerling is cultivated and seed transport production step.
2. a kind of river batch production offspring seed cultivation method according to claim 1, it is characterised in that: during described parent fish rearing, the native pool area 2000-6000m cultivated2, the depth of water is at 1.2-1.5m；Indoor temperature control cement pit area is 50-80m2, the depth of water adopts circulating filtration and vortex air pump oxygen supplement at 0.6-0.8m, Tu Chi, it is ensured that Dissolved Oxygen in Water is at more than 5mg/L, and salinity is between 10-15 ‰, and water temperature is between 18-25 DEG C, and soil pond lofting amount is 0.2-0.3kg/m2, cement pit lofting amount is 1.5-2kg/m2, female-male proportion 1.5:1, based on fresh food, eel compound feed of arranging in pairs or groups, adds appropriate vitamin and nutritive salt promotes river gonad development, and feeding volume is the 2-3% of river body weight, sooner or later respectively throws something and feeds once, adopts food platform to throw something and feed.
3. a kind of river batch production offspring seed cultivation method according to claim 1, it is characterized in that: during the screening of described parent fish, selection physique is strong, desalination is abundant, the parent fish parent fish in support of gonadal maturation, more than raun 3 age, body weight more than 750 grams, abdominal part expands, and cloacal aperture is micro-red and lax；More than milter 2 age, body weight more than 450 grams, abdominal part is slightly loose soft, presses fish body abdominal part to have milky seminal fluid to flow out with have gentle hands.
4. a kind of river batch production offspring seed cultivation method according to claim 1, it is characterized in that: in described artificial induced spawning, different according to gonad development degree, the oxytocic hormone of injection various dose, inject hasten parturition LRH-A hormone and Medulla Cyprinus carpio hypophysis, LRH-A hormone dosage of hastening parturition is 8 μ g, Medulla Cyprinus carpio hypophysis 1-2/tail, point biphasic injection, first time injects the 1/4 of accumulated dose, second time injects surplus, adopts muscle of back injection or intrathecal.
5. a kind of river batch production offspring seed cultivation method according to claim 1, it is characterized in that: during artificial insemination, dry parent fish body surface moisture, fish roe is extracted in dry smooth container, rapidly milter seminal fluid is squeezed on ovum simultaneously, it is gently mixed uniformly with banister brush, makes essence ovum be sufficiently mixed, then add clear water stirring, after standing 2-3 minute, changing water 2-3 time, clear to water, whole operating process completes in 30min.
6. a kind of river batch production offspring seed cultivation method according to claim 1, it is characterized in that: during described artificial incubation, incubating oosperm mode mainly adopts hatching barrel Lotic hatching and viscous ovum plate Lentic hatching, hatching barrel Lotic hatching: be sewn into cone with nylon mesh tulle, mesh size is 250 μm, diameter 60cm, height 80cm, bottom is funnel-form, it is placed in cement pit, bottom inflatable, Lotic hatching, hatching density is 1500-2000 grain/L；Viscous ovum plate Lentic hatching: make the framework of long 80-100cm, wide 40-60cm with batten, it is sewn on framework makes viscous ovum plate with cotton gauze or nylon bolting silk that mesh size is 250 μm, Lentic hatching is put into by sticking the viscous ovum plate having germ cell, hatching density: every square centimeter of uniform adhesion germ cell 3-4 grain on viscous ovum plate, in whole hatching process, temperature rests in 18-22 DEG C, and illumination controls at 600-1000lx, and oxygen in water is more than 6mg/L.
7. a kind of river batch production offspring seed cultivation method according to claim 5 or 6, it is characterised in that: when artificial induced spawning and artificial incubation, temperature at 18 DEG C-22 DEG C, dissolved oxygen in water 6-8mg/L, use the water body of salubrious circulation.
8. a kind of river batch production offspring seed cultivation method according to claim 1, it is characterized in that: when factory fry is cultivated, river is cultivated under water temperature 18-32 DEG C of condition, takes the low illumination of early stage, the principle of later stage height illumination, controls water temperature and illumination with two-layer sunshade net；Putting Seedling the last week, improve the Biomass of pond zooplankton by using bio-fertilizer, fry enters before pond with the sodium chloride solution sterilization 10-15min of 2%-3%, and early stage cultivates density at 1000-2000 tail/m3, the later stage cultivates density at 150-250 tail/m3, adopt chlorella organic debris cultivation wheel animalcule, blood worm duty to feed, the transition period ensures that bait is sufficient, agreeable to the taste, when later stage cultivates first in great Chi, fill band chlorella, wheel animalcule, blood worm 10 centimeters of old water deep, then, add new water to 40 centimeters, splash Semen Glycines soybean milk 20 liter/day, splashing three, after five days, water is light green color, defines microorganisms in water chain, having certain self-purification capacity, then band water is transplanted seedlings again；Entering later stage cultivation, accomplish there is complementarity living bait in pond, Seedling is cultivated the dissolved oxygen in water needed and is kept 6-8mg/L, and ammonia-nitrogen content is at 0-1mg/L, and during later stage nursery, can disposable splash lime water 20-40ppm on the every ten.
9. a kind of river batch production offspring seed cultivation method according to claim 8, it is characterised in that: when outdoor large size fingerling is cultivated, cultivate fingerling, breeding density 10-30 tail/m with cement pit2, stocking size is 30-100mm, and the culture-cycle is 60-150 days, and forming specification is 150-250mm.
10. a kind of river batch production offspring seed cultivation method according to claim 1, it is characterized in that: in seed transport, take the water shipment seed in harvesting waters, dissolved oxygen is not less than 6mg/L in transit, transport water temperature changes less than 5 DEG C, adopting live fish car transport seed, haulage time was less than 10 hours, and fish and water ratio is not more than 1:80.
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| CN114027229A (en) * | 2021-11-30 | 2022-02-11 | 中国水产科学研究院黄海水产研究所 | Novel method for cultivating takifugu rubripes fries |
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