CN105624245A - 一种胶原蛋白的修饰改性方法 - Google Patents
一种胶原蛋白的修饰改性方法 Download PDFInfo
- Publication number
- CN105624245A CN105624245A CN201610093509.4A CN201610093509A CN105624245A CN 105624245 A CN105624245 A CN 105624245A CN 201610093509 A CN201610093509 A CN 201610093509A CN 105624245 A CN105624245 A CN 105624245A
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- Prior art keywords
- collagen protein
- collagen
- modification method
- catalyst
- acetic acid
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- 102000008186 Collagen Human genes 0.000 title claims abstract description 84
- 108010035532 Collagen Proteins 0.000 title claims abstract description 84
- 238000002715 modification method Methods 0.000 title claims abstract description 22
- 229920001436 collagen Polymers 0.000 title abstract description 13
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 57
- 239000003054 catalyst Substances 0.000 claims abstract description 25
- 229920001661 Chitosan Polymers 0.000 claims abstract description 21
- 102000035195 Peptidases Human genes 0.000 claims abstract description 19
- 108091005804 Peptidases Proteins 0.000 claims abstract description 19
- 238000010438 heat treatment Methods 0.000 claims abstract description 18
- RSEIMSPAXMNYFJ-UHFFFAOYSA-N europium(III) oxide Inorganic materials O=[Eu]O[Eu]=O RSEIMSPAXMNYFJ-UHFFFAOYSA-N 0.000 claims abstract description 6
- CMIHHWBVHJVIGI-UHFFFAOYSA-N gadolinium(III) oxide Inorganic materials [O-2].[O-2].[O-2].[Gd+3].[Gd+3] CMIHHWBVHJVIGI-UHFFFAOYSA-N 0.000 claims abstract description 6
- FKTOIHSPIPYAPE-UHFFFAOYSA-N samarium(III) oxide Inorganic materials [O-2].[O-2].[O-2].[Sm+3].[Sm+3] FKTOIHSPIPYAPE-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000012047 saturated solution Substances 0.000 claims description 26
- 239000000243 solution Substances 0.000 claims description 19
- 239000000203 mixture Substances 0.000 claims description 8
- 241000251468 Actinopterygii Species 0.000 claims description 6
- 239000000284 extract Substances 0.000 claims description 6
- 241000191442 Tenualosa reevesii Species 0.000 claims description 4
- 241000283690 Bos taurus Species 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 235000013305 food Nutrition 0.000 abstract description 5
- 239000003814 drug Substances 0.000 abstract description 4
- 238000004132 cross linking Methods 0.000 abstract description 3
- 229920006395 saturated elastomer Polymers 0.000 abstract 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 abstract 1
- 230000007547 defect Effects 0.000 abstract 1
- 235000015110 jellies Nutrition 0.000 abstract 1
- 239000008274 jelly Substances 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 230000000052 comparative effect Effects 0.000 description 6
- 239000000463 material Substances 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- AZKVWQKMDGGDSV-BCMRRPTOSA-N Genipin Chemical compound COC(=O)C1=CO[C@@H](O)[C@@H]2C(CO)=CC[C@H]12 AZKVWQKMDGGDSV-BCMRRPTOSA-N 0.000 description 2
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 2
- 241000282898 Sus scrofa Species 0.000 description 2
- 238000010382 chemical cross-linking Methods 0.000 description 2
- 239000003431 cross linking reagent Substances 0.000 description 2
- AZKVWQKMDGGDSV-UHFFFAOYSA-N genipin Natural products COC(=O)C1=COC(O)C2C(CO)=CCC12 AZKVWQKMDGGDSV-UHFFFAOYSA-N 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 1
- 101000933967 Pseudomonas phage KPP25 Major capsid protein Proteins 0.000 description 1
- 241000282894 Sus scrofa domesticus Species 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000023555 blood coagulation Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 210000004292 cytoskeleton Anatomy 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 239000007943 implant Substances 0.000 description 1
- 239000010985 leather Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- CSNNHWWHGAXBCP-UHFFFAOYSA-L magnesium sulphate Substances [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 238000011056 performance test Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Toxicology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Description
实施例序号 | 弹性模量(mPa) | 黏性模量(mPa) |
实施例1 | 830 | 5350 |
实施例2 | 800 | 5300 |
实施例3 | 790 | 5200 |
实施例4 | 850 | 5500 |
实施例5 | 860 | 5500 |
对比例1 | 650 | 3500 |
对比例2 | 670 | 3800 |
Claims (6)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610093509.4A CN105624245B (zh) | 2016-02-19 | 2016-02-19 | 一种胶原蛋白的修饰改性方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610093509.4A CN105624245B (zh) | 2016-02-19 | 2016-02-19 | 一种胶原蛋白的修饰改性方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN105624245A true CN105624245A (zh) | 2016-06-01 |
CN105624245B CN105624245B (zh) | 2020-05-22 |
Family
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN201610093509.4A Active CN105624245B (zh) | 2016-02-19 | 2016-02-19 | 一种胶原蛋白的修饰改性方法 |
Country Status (1)
Country | Link |
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CN (1) | CN105624245B (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109400700A (zh) * | 2018-11-06 | 2019-03-01 | 安徽怡发生物科技有限公司 | 一种易吸收的胶原蛋白低聚肽的制备方法 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100998897A (zh) * | 2006-12-27 | 2007-07-18 | 天津大学 | 具有双重可控释放涂层的可吸收镁合金支架及其制备方法 |
CN101798345A (zh) * | 2009-02-05 | 2010-08-11 | 颜建华 | 一种修饰胶原蛋白的方法 |
CN103819685A (zh) * | 2014-03-11 | 2014-05-28 | 武汉理工大学 | 鱼鳞胶原蛋白接枝壳聚糖及其制备方法、用途 |
-
2016
- 2016-02-19 CN CN201610093509.4A patent/CN105624245B/zh active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100998897A (zh) * | 2006-12-27 | 2007-07-18 | 天津大学 | 具有双重可控释放涂层的可吸收镁合金支架及其制备方法 |
CN101798345A (zh) * | 2009-02-05 | 2010-08-11 | 颜建华 | 一种修饰胶原蛋白的方法 |
CN103819685A (zh) * | 2014-03-11 | 2014-05-28 | 武汉理工大学 | 鱼鳞胶原蛋白接枝壳聚糖及其制备方法、用途 |
Non-Patent Citations (1)
Title |
---|
徐新宇: "胶原的提取、改性、交联及其应用", 《透析与人工器官》 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109400700A (zh) * | 2018-11-06 | 2019-03-01 | 安徽怡发生物科技有限公司 | 一种易吸收的胶原蛋白低聚肽的制备方法 |
Also Published As
Publication number | Publication date |
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CN105624245B (zh) | 2020-05-22 |
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Effective date of registration: 20160713 Address after: 215100 Suzhou, Wuzhong District, Li Feng commercial center, block, room 2, building 2406, room A Applicant after: Shimao (Suzhou) Biotechnology Co., Ltd. Address before: Wuzhong District Wuzhong Economic Development Zone Suzhou city Jiangsu province 215104 the River Street Wuzhong Road No. 2599 Building 1, 4 floor, room 406, 405 404 Applicant before: SUZHOU WEICO BIOTECHNOLOGY CO., LTD. |
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Effective date of registration: 20200423 Address after: No.2, Hangkong Road, Wangcheng economic and Technological Development Zone, Changsha City, Hunan Province Applicant after: Hunan lifeyuan Medicine Co., Ltd Address before: 215100 Suzhou, Wuzhong District, Li Feng commercial center, block, room 2, building 2406, room A Applicant before: SHIMAO (SUZHOU) BIOTECHNOLOGY Co.,Ltd. |
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Effective date of registration: 20210917 Address after: 410200 No.2 Hangkong Road, Wangcheng economic and Technological Development Zone, Changsha City, Hunan Province Patentee after: Hunan life yuan special Medical Biotechnology Co.,Ltd. Address before: 410200 No.2 Hangkong Road, Wangcheng economic and Technological Development Zone, Changsha City, Hunan Province Patentee before: Hunan lifeyuan Medicine Co.,Ltd. |
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