CN105409839B - A kind of mastacembelus aculeatus artificial fecundation method - Google Patents
A kind of mastacembelus aculeatus artificial fecundation method Download PDFInfo
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- CN105409839B CN105409839B CN201510812385.6A CN201510812385A CN105409839B CN 105409839 B CN105409839 B CN 105409839B CN 201510812385 A CN201510812385 A CN 201510812385A CN 105409839 B CN105409839 B CN 105409839B
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- 241000981894 Macrognathus aculeatus Species 0.000 title claims abstract description 52
- 238000000034 method Methods 0.000 title claims abstract description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 26
- 239000003814 drug Substances 0.000 claims abstract description 13
- 229940079593 drug Drugs 0.000 claims abstract description 13
- 230000012447 hatching Effects 0.000 claims abstract description 12
- 238000009395 breeding Methods 0.000 claims abstract description 6
- 230000001488 breeding effect Effects 0.000 claims abstract description 6
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 5
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 5
- 230000009027 insemination Effects 0.000 claims abstract description 5
- 210000004681 ovum Anatomy 0.000 claims abstract description 5
- 241000143060 Americamysis bahia Species 0.000 claims abstract description 3
- 241000361919 Metaphire sieboldi Species 0.000 claims abstract description 3
- 241000133262 Nauplius Species 0.000 claims abstract description 3
- 238000002347 injection Methods 0.000 claims description 9
- 239000007924 injection Substances 0.000 claims description 9
- 239000012530 fluid Substances 0.000 claims description 8
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 6
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 6
- 235000013601 eggs Nutrition 0.000 claims description 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 5
- 239000001301 oxygen Substances 0.000 claims description 5
- 229910052760 oxygen Inorganic materials 0.000 claims description 5
- 239000003755 preservative agent Substances 0.000 claims description 5
- 230000002335 preservative effect Effects 0.000 claims description 5
- 230000002196 ecbolic effect Effects 0.000 claims description 4
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 3
- 241000196324 Embryophyta Species 0.000 claims description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- 239000001110 calcium chloride Substances 0.000 claims description 3
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 3
- 239000000919 ceramic Substances 0.000 claims description 3
- FPAFDBFIGPHWGO-UHFFFAOYSA-N dioxosilane;oxomagnesium;hydrate Chemical compound O.[Mg]=O.[Mg]=O.[Mg]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O FPAFDBFIGPHWGO-UHFFFAOYSA-N 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 239000004570 mortar (masonry) Substances 0.000 claims description 3
- 210000003205 muscle Anatomy 0.000 claims description 3
- 239000001103 potassium chloride Substances 0.000 claims description 3
- 235000011164 potassium chloride Nutrition 0.000 claims description 3
- 208000026487 Triploidy Diseases 0.000 claims description 2
- 238000001914 filtration Methods 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims description 2
- 230000000638 stimulation Effects 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims description 2
- 238000003860 storage Methods 0.000 claims description 2
- KZBUYRJDOAKODT-UHFFFAOYSA-N Chlorine Chemical compound ClCl KZBUYRJDOAKODT-UHFFFAOYSA-N 0.000 claims 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims 1
- 239000000460 chlorine Substances 0.000 claims 1
- 229910052801 chlorine Inorganic materials 0.000 claims 1
- 239000011734 sodium Substances 0.000 claims 1
- 229910052708 sodium Inorganic materials 0.000 claims 1
- 230000004720 fertilization Effects 0.000 abstract description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 210000001015 abdomen Anatomy 0.000 description 6
- 210000004907 gland Anatomy 0.000 description 6
- 230000001568 sexual effect Effects 0.000 description 6
- 210000002700 urine Anatomy 0.000 description 6
- 241000251468 Actinopterygii Species 0.000 description 4
- 241000252185 Cobitidae Species 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 210000001647 gastrula Anatomy 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 3
- 241000276618 Perciformes Species 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
- 239000004568 cement Substances 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000013505 freshwater Substances 0.000 description 2
- 210000002149 gonad Anatomy 0.000 description 2
- 230000026109 gonad development Effects 0.000 description 2
- 230000036571 hydration Effects 0.000 description 2
- 238000006703 hydration reaction Methods 0.000 description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 2
- 230000032696 parturition Effects 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 241000357443 Mastacembelidae Species 0.000 description 1
- 206010027336 Menstruation delayed Diseases 0.000 description 1
- 210000002298 blastodisc Anatomy 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000002224 dissection Methods 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 210000000472 morula Anatomy 0.000 description 1
- 239000008239 natural water Substances 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000009303 sperm storage Effects 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a kind of mastacembelus aculeatus artificial fecundation method, at the beginning of annual Breeding climax period, i.e., annual mid-May to 7 months and September part, the good parent population of sexal maturity, female-male proportion 1: 1.2 are picked out from wild capture or domestic mastacembelus aculeatus；Then artificial induced spawning drug is injected to parent population, the sperm and ovum for taking milter and raun respectively carry out artificial insemination, are open after hatching with shrimps nauplius, feed foot one month with water earthworm later, long to 3 centimetres or so, start to be tamed with mixed feed.Artificial fecundation method disclosed in this invention has the features such as high spawning rate, high rate of fertilization, high hatchability, high-survival rate, while also has the characteristics that parent's death rate is low.It is suitable to carry out the breeding of scale mastacembelus aculeatus seed on demand, there is efficient, simplicity, conducive to popularization.
Description
Technical field
The invention belongs to technical field of aquaculture, and in particular to a kind of artificial fecundation method of mastacembelus aculeatus.
Technical background
Mastacembelus aculeatus (Mastacembelue aculeatus) is subordinate to Perciformes (Perciformes), mastacembelus aculeatus section in classification
(Mastacembelidae), mastacembelus aculeatus category (Mastacembelue Scopoli).It is commonly called as knife loach, thorn loach, husky loach, pig mother saw
Deng, meat exquisiteness, nutritive value height.It is the small-sized economic freshwater fish based on carnivorous, Natural Population, which likes to inhabit in, to be had
The rivers bottom of stone or bank have at water plant.It does not like more suddenly to locate life in flow in natural water area.In the Zhejiang of the Yangtze river basin
River, Jiangsu, Hubei, Hunan and Henan almost spread over the whole province waters.Due in recent years to its fishing without restraint and its nature
The variation of water body environment condition, mastacembelus aculeatus Natural Population quantity significantly reduce, state that market shows that supply falls short of demand.Therefore, development thorn
Loach aquaculture is of great significance.At present restrict mastacembelus aculeatus large-scale cultivation factor, in addition to cultural technique, feed or disease these
Outside factor, most important is exactly the artificial propagation problem of mastacembelus aculeatus seed, lacks systematicness to the artificial propagation of mastacembelus aculeatus seed at present
Research, causes the artificial propagation spawning rate of mastacembelus aculeatus seed, rate of fertilization and hatching rate are very low.
Invention content
The purpose of the present invention is to provide a kind of artificial fecundation method of mastacembelus aculeatus, the artificial propagation difficult point for solving mastacembelus aculeatus is asked
Topic improves the spawning rate of mastacembelus aculeatus artificial propagation, rate of fertilization and hatching rate.
For achieving the above object, present invention employs following technical schemes：
A kind of mastacembelus aculeatus artificial fecundation method, it includes the following steps：
(1) at the beginning of annual Breeding climax period, i.e., annual mid-May to 7 months and September part, from wild capture or domestic
Pick out the good parent population of sexal maturity in mastacembelus aculeatus, female requires more than 30g, gonopore it is round it is rubescent is preferred, male require 50g with
On, sperm can be squeezed out and be preferred, female-male proportion 1: 1.2；
(2) artificial induced spawning drug is injected to parent population, male and female parent population injection dosage is identical, the type and note of artificial induced spawning drug
Dosage is penetrated as HCG 1500IU/kg+LRH-A250μg/kg+DOM 5mg/kg；Male and female parent population is injected using two needle of muscle of back
Method, the first needle only inject LRH-A2, dosage is the 1/5-2/5 of accumulated dose, after being spaced 12h, the second needle is injected, by remaining oxytocic
Object injection finishes；
(3) parent population after injection artificial induced spawning drug is placed in shading and stimulation by running water in storage pond, holding pond, keeps 26 DEG C of water temperature,
More than dissolved oxygen 5mg/L；
(4) it during artificial insemination, first dissects milter and takes out spermary, be placed on 80 mesh gauzes, ground with mortar, be then added dropwise
A small amount of spermatozoa preservative fluid, filtering, it is sperm to collect filtered fluid；The mature egg of raun is squeezed into dry ceramic bowl and is added dropwise
Enter sperm, stir, with yellow mud pulp-water or talcum powder unsticking 3 minutes after sperm and ovum mixing；
(5) fertilized eggs after unsticking are placed in hatchery and are protected from light hatching, keep 26 DEG C of water temperature, more than dissolved oxygen 5mg/L is micro-
Flowing water, changes water 1/5th daily, and triploidy rate is 3000/m2, hatch pool area and should not be too large, general length and width are 4
~5m, pond is deep preferably 1m, is sterilized before use；
(6) fry hatching sets water plant into hatchery and hides object, be open with shrimps nauplius after 5 days, and one
It feeds 6 times, persistently feeds 8 to 10 days, feeds foot one month with water earthworm later, long to 3 centimetres or so, starts to use mixed feed
Domestication.
Preferably, the formula of the spermatozoa preservative fluid is：Sodium chloride 7.5g, potassium chloride 0.3g, calcium chloride 0.3g, seven hydrations
Magnesium sulfate 0.3g, glucose 3.0g, distilled water constant volume to 1000ml.
The present invention carries out system research to the artificial propagation of mastacembelus aculeatus for the first time, and the artificial fecundation method provided is hastened parturition with height
The features such as rate, high rate of fertilization, high hatchability, high-survival rate, while also have the characteristics that parent's death rate is low.Suitable for opening on demand
The breeding of scale mastacembelus aculeatus seed is opened up, there is efficient, simplicity, conducive to popularization.
Description of the drawings
Fig. 1 is mastacembelus aculeatus urine bubble photo (arrow signified), in figure：A is male mastacembelus aculeatus urine bubble；B is female mastacembelus aculeatus urine bubble.
Fig. 2 is that mastacembelus aculeatus sexual gland is sliced, in figure：A is female mastacembelus aculeatus sexual gland；B is male mastacembelus aculeatus sexual gland.
Fig. 3 is male and female mastacembelus aculeatus gonad development state Monthly changes curve, in figure：A is in Section IV phase or V for female gonads
Number of individuals account for the percentage of total individual number；B is that male gonad is in the number of individuals of Section IV phase or V and accounts for the percentage of total individual number
Than.
Fig. 4 is mastacembelus aculeatus embryo development procedure, a- fertilized eggs；B- blastodiscs swell；C-2 cell stages；D-4 cell stages；E-
8 cell stages；F-16 cell stages；G- multicellular stages；H- morula stages；I- blastaeas early stage；J- blastaea mid-terms；K- blastaeas evening
Phase；L- primitive guts early stage；M- primitive gut mid-terms；N- primitive gut late periods；O- neurula stages；P- blastopores are closed the phase；Q- body segments occur
Phase；R- base apparitions；The s- eye capsule phases；The t- tail bud phases；The u- body colours element apparition；The v- crystal apparitions；W-muscle effect
Ying Qi；X-heart beat period；Y-membrane phase.
Specific embodiment
Inventor is carried out in June, 2014 in July, 2015 in marine products institute of Hua Zhong Agriculture University using the method for the present invention
Mastacembelus aculeatus artificial propagation techniques are studied, and breeding parent in field capture individual by screening.
In artificial propagation of fish practice production, the male and female differentiation of parent is the premise of large-scale production.But mastacembelus aculeatus is female
Male individual differentiation is more difficult, is one of the difficult point of mastacembelus aculeatus artificial propagation.Although male is noticeably greater than female in mastacembelus aculeatus of the same age
Individual, but ages differ in capture individual in the wild, and Individual Size cannot function as judgement male and female sole criterion.And in reproduction
In season, as female individuals, male abdomen expands softness, and does not find the secondary sex characters such as similar star-pursuing.Inventor
Find that there is healthy and strong male huge urine to steep without exception by dissecting more than 300 mastacembelus aculeatus, female urine bubble is then smaller, such as Fig. 1.Therefore
In light crowded abdomen, male amount of urine is big and in spurting, and inventor is in this, as one of standard for differentiating male and female, in conjunction with a
Body size and can squeeze out sperm as the present invention in differentiate mastacembelus aculeatus male and female method.
It is also one of mastacembelus aculeatus artificial propagation difficult point that well-developed mastacembelus aculeatus parent, which obtains,.Carry out sexual gland dissection within continuous multiple months
It has been observed that thin-line-shaped or cylindric (pink) was only presented in male mastacembelus aculeatus spermary, do not find such as other most freshwater fishes
The milky presented when Section IV phase or V phase spermary, and have the outflow of a large amount of sperm during light pressure.Therefore, male mastacembelus aculeatus is selected to make
Month to breed parent is difficulties, inventor by the way that the sexual gland of field capture individual is dissected and observed at random and
Histotomy is observed (see Fig. 2), sums up multiple month male and female mastacembelus aculeatus gonad development situations (see Fig. 3).Based on result above, hair
During person of good sense's selection is at the beginning of annual mid-May to 7 months or during entire September part, male and female mastacembelus aculeatus sexual gland is developed preferably
Period is as the time for selecting mastacembelus aculeatus parent.
Inventor to parent by carrying out artificial induced spawning, fertilization, hatching, and successively many successes breed mastacembelus aculeatus, mastacembelus aculeatus
Embryonic result is shown in Fig. 4.Now enumerate specific embodiment three times：
Embodiment 1
1. the selection of parent
98 good parent populations of sexal maturity have been picked out in the mastacembelus aculeatus individual of wild capture.Female more than 30g, abdomen are swollen
Big soft, gonopore circle is rubescent, male more than 50g, and healthy and strong flexible, majority can squeeze out sperm.Female-male proportion is 1：1.
2. mode of hastening parturition
Artificial induced spawning drug includes HCG, LRH-A2And DOM.Oxytocic drug dosage is shown in Table 1.Artificial induced spawning drug further includes
The sodium chloride solution of every kilogram of parent population 0.3ml 0.7%.Induced spawning method is two needle injection of muscle of back, and the first needle is only injected
LRH-A2, it is divided between the injection time of the second needle 12 hours.
3. artificial insemination method
Parent population is checked every two hours, gently crowded abdomen has the outflow of ovum grain that can carry out artificial insemination after 10 hours.
It dissects milter and takes out spermary, be placed on 80 mesh gauzes, a small amount of spermatozoa preservative fluid mistake is added dropwise after being ground with mortar
Filter, it is sperm to collect filtered fluid.Sperm storage formula of liquid：Sodium chloride 7.5g, potassium chloride 0.3g, calcium chloride 0.3g, seven hydrations
Magnesium sulfate 0.3g, glucose 3.0g, distilled water constant volume to 1000ml.Mature egg is squeezed into dropwise addition sperm in dry ceramic bowl to mix
It is even.
4. method for incubating oosperm
It treats after sperm and ovum mixing with yellow mud pulp-water or talcum powder unsticking 3 minutes.It is placed in hatchery and is protected from light hatching,
26 DEG C, more than dissolved oxygen 5mg/L of water temperature is kept, miniflow water changes water 1/5th, 3000/m of fish-egg density daily2.Hatch cement
Pool area should not be too large, generally 4mX4m (long X wide) or 5mX5m (long X wide).Cement pit pond is deep preferably 1m.
Hatchery is used as nursery pond.Mastacembelus aculeatus spray stage constitution is weaker, can be reduced by the use of hatchery as nursery pond and turn fish
The damage being subject in the process improves survival rate.
Embodiment 2
112 good parent populations of sexal maturity have been picked out in the mastacembelus aculeatus individual of wild capture.Female more than 30g, abdomen
Softness is expanded, gonopore circle is rubescent, male more than 50g, and healthy and strong flexible, majority can squeeze out sperm.Female-male proportion is 1：1.2.
Other each steps are same as Example 1.
Embodiment 3
70 good parent populations of sexal maturity have been picked out in the mastacembelus aculeatus individual of wild capture.Female more than 30g, abdomen are swollen
Big soft, gonopore circle is rubescent, male more than 50g, and healthy and strong flexible, majority can squeeze out sperm.Female-male proportion is 1：1.1.Its
Its each step is same as Example 1.
Examples 1 to 3 and artificial induced spawning drug dosage pairing effect time, parent's spawning rate, rate of fertilization, parent's death
The influence of 7 days survival rates is shown in Table 1 after rate, hatching rate and hatching.
The influence of the mastacembelus aculeatus reproductive effect of 1 oxytocic drug dosage pair of table
Claims (2)
1. a kind of mastacembelus aculeatus artificial fecundation method, it is characterised in that include the following steps：
(1) at the beginning of annual Breeding climax period, i.e., annual mid-May to 7 months and September part, from wild capture or domestic mastacembelus aculeatus
In pick out the good parent population of sexal maturity, female requires more than 30g, gonopore it is round it is rubescent is preferred, male requires more than 50g,
Sperm can be squeezed out to be preferred, female-male proportion 1: 1.2；
(2) artificial induced spawning drug is injected to parent population, male and female parent population injection dosage is identical, the type and injection of artificial induced spawning drug
It measures as HCG 1500IU/kg+LRH-A250μg/kg+DOM 5mg/kg；Male and female parent population uses two needle injection of muscle of back,
First needle only injects LRH-A2, dosage is the 2/5 of accumulated dose, after being spaced 12h, injects the second needle, remaining oxytocic drug is injected
It finishes；
(3) parent population after injection artificial induced spawning drug is placed in shading and stimulation by running water in storage pond, holding pond, keeps 26 DEG C of water temperature, dissolved oxygen
More than 5mg/L；
(4) it during artificial insemination, first dissects milter and takes out spermary, be placed on 80 mesh gauzes, ground with mortar, be then added dropwise a small amount of
Spermatozoa preservative fluid, filtering, it is sperm to collect filtered fluid；The mature egg of raun is squeezed into dry ceramic bowl and is added dropwise to essence
Liquid, stirring, with yellow mud pulp-water or talcum powder unsticking 3 minutes after sperm and ovum mixing；
(5) fertilized eggs after unsticking are placed in hatchery and are protected from light hatching, keep 26 DEG C, more than dissolved oxygen 5mg/L of water temperature, miniflow
Water, changes water 1/5th daily, and triploidy rate is 3000/m2, hatching pool area should not be too large, and general length and width are 4~
5m, pond is deep preferably 1m, is sterilized before use；
(6) fry hatching sets water plant into hatchery and hides object, be open with shrimps nauplius, thrown within one day after 5 days
It feeds 6 times, persistently feeds 8 to 10 days, feed foot one month with water earthworm later, it is long to 3 centimetres or so, start to be tamed and dociled with mixed feed
Change.
2. mastacembelus aculeatus artificial fecundation method as described in claim 1, it is characterised in that：The formula of the spermatozoa preservative fluid is：Chlorine
Change sodium 7.5g, potassium chloride 0.3g, calcium chloride 0.3g, bitter salt 0.3g, glucose 3.0g, distilled water constant volume arrives
1000ml。
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN204377691U (en) * | 2014-12-24 | 2015-06-10 | 福建省淡水水产研究所 | A kind of large mastacembelus aculeatus perches platform |
Non-Patent Citations (4)
| Title |
|---|
| 大刺鳅人工繁殖技术初探;张建铭等;《中国水产》;20150930(第9期);第85-86页 * |
| 大刺鳅人工繁殖研究初报;李晓双;《中国水产》;20070531(第5期);第44-46页 * |
| 姜彦超等.俄罗斯鲟雌核发育诱导及其后代的微卫星分析.《中山大学学报》.2012,第51卷(第4期),第106-111及133页. * |
| 黄永亨.泥鳅人工高效繁育与养殖技术.《科学养鱼》.2012,(第8期),第34-35页. * |
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| CN105409839A (en) | 2016-03-23 |
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