CN105165692A - Method for anesthetizing loach parent - Google Patents
Method for anesthetizing loach parent Download PDFInfo
- Publication number
- CN105165692A CN105165692A CN201510700202.1A CN201510700202A CN105165692A CN 105165692 A CN105165692 A CN 105165692A CN 201510700202 A CN201510700202 A CN 201510700202A CN 105165692 A CN105165692 A CN 105165692A
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- China
- Prior art keywords
- loach
- parent
- loach parent
- plastic basin
- clear water
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- 241000252185 Cobitidae Species 0.000 title claims abstract description 59
- 238000000034 method Methods 0.000 title claims abstract description 24
- 230000003444 anaesthetic effect Effects 0.000 claims abstract description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 20
- 230000002196 ecbolic effect Effects 0.000 claims abstract description 6
- 230000009191 jumping Effects 0.000 claims abstract description 5
- 239000003814 drug Substances 0.000 claims description 5
- 229940079593 drug Drugs 0.000 claims description 5
- 235000013601 eggs Nutrition 0.000 claims description 4
- AFVFQIVMOAPDHO-UHFFFAOYSA-N Methanesulfonic acid Chemical compound CS(O)(=O)=O AFVFQIVMOAPDHO-UHFFFAOYSA-N 0.000 claims description 2
- 125000004494 ethyl ester group Chemical group 0.000 claims description 2
- XLXSAKCOAKORKW-AQJXLSMYSA-N gonadorelin Chemical class C([C@@H](C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N1[C@@H](CCC1)C(=O)NCC(N)=O)NC(=O)[C@H](CO)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H](CC=1N=CNC=1)NC(=O)[C@H]1NC(=O)CC1)C1=CC=C(O)C=C1 XLXSAKCOAKORKW-AQJXLSMYSA-N 0.000 claims description 2
- 239000002434 gonadorelin derivative Substances 0.000 claims description 2
- 230000003533 narcotic effect Effects 0.000 claims description 2
- 206010002091 Anaesthesia Diseases 0.000 abstract description 22
- 230000037005 anaesthesia Effects 0.000 abstract description 20
- FQZJYWMRQDKBQN-UHFFFAOYSA-N tricaine methanesulfonate Chemical compound CS([O-])(=O)=O.CCOC(=O)C1=CC=CC([NH3+])=C1 FQZJYWMRQDKBQN-UHFFFAOYSA-N 0.000 abstract description 5
- 238000002347 injection Methods 0.000 abstract description 2
- 239000007924 injection Substances 0.000 abstract description 2
- 230000006698 induction Effects 0.000 abstract 1
- 229940013180 tricaine methanesulfonate Drugs 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 7
- 230000032696 parturition Effects 0.000 description 4
- 238000001949 anaesthesia Methods 0.000 description 3
- 235000019688 fish Nutrition 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 2
- RRAFCDWBNXTKKO-UHFFFAOYSA-N eugenol Chemical compound COC1=CC(CC=C)=CC=C1O RRAFCDWBNXTKKO-UHFFFAOYSA-N 0.000 description 2
- 230000035558 fertility Effects 0.000 description 2
- 230000029058 respiratory gaseous exchange Effects 0.000 description 2
- 241000208340 Araliaceae Species 0.000 description 1
- NPBVQXIMTZKSBA-UHFFFAOYSA-N Chavibetol Natural products COC1=CC=C(CC=C)C=C1O NPBVQXIMTZKSBA-UHFFFAOYSA-N 0.000 description 1
- 241000252203 Clupea harengus Species 0.000 description 1
- 241001014350 Cynoglossus semilaevis Species 0.000 description 1
- TWLLPUMZVVGILS-UHFFFAOYSA-N Ethyl 2-aminobenzoate Chemical compound CCOC(=O)C1=CC=CC=C1N TWLLPUMZVVGILS-UHFFFAOYSA-N 0.000 description 1
- 239000005770 Eugenol Substances 0.000 description 1
- 241001110084 Hilsa Species 0.000 description 1
- 241000277275 Oncorhynchus mykiss Species 0.000 description 1
- 241000277277 Oncorhynchus nerka Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 241001529596 Pontinus kuhlii Species 0.000 description 1
- UVMRYBDEERADNV-UHFFFAOYSA-N Pseudoeugenol Natural products COC1=CC(C(C)=C)=CC=C1O UVMRYBDEERADNV-UHFFFAOYSA-N 0.000 description 1
- 241000404975 Synchiropus splendidus Species 0.000 description 1
- 241001633982 Verasper Species 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 229960002217 eugenol Drugs 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 235000019514 herring Nutrition 0.000 description 1
- WSZKUEZEYFNPID-UHFFFAOYSA-N hydrogen sulfate;quinolin-1-ium Chemical compound OS(O)(=O)=O.N1=CC=CC2=CC=CC=C21 WSZKUEZEYFNPID-UHFFFAOYSA-N 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 231100000225 lethality Toxicity 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
- 231100000989 no adverse effect Toxicity 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Veterinary Medicine (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Engineering & Computer Science (AREA)
- Marine Sciences & Fisheries (AREA)
- Wood Science & Technology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a method for efficiently anesthetizing a loach parent. The method includes the steps that A, a selected loach parent is fished out, and is placed in a prepared plastic basin after being drained off; B, anesthetic is dissolved in clear water, and is poured into the plastic basin after being dissolved; C, the loach parent reacts fiercely, the upper portion of the plastic basin is covered with another plastic basin so as to prevent the loach parent from jumping out, and anesthesia is completed after 3-5 minutes; D, oxytocic begins to be injected, the loach parent is placed in clear water after injection is completed, and after reviving completely in 5-10 minutes, the loach parent is placed in spawning equipment for awaiting delivery; tricaine methanesulfonate is selected to serve as the anesthetic; the using dosage of the anesthetic is 10 mg/kg, and the anesthetic is dissolved in 10-30 ml of clear water in the using process. The method is easy to implement and simple and convenient to operate, the fast anesthetic effect is achieved through the skin permeation action, and therefore the spawning induction efficiency of loaches is improved obviously.
Description
Technical field
The present invention relates to aquaculture field, particularly relate to a kind of method that loach parent efficiently anaesthetizes, also can be used for loach and to belong to and secondary loach belongs to the anesthesia of other kinds.
Technical background
Loach is one of breed variety that China is emerging in recent years, and market demand increases year by year, cultivation upsurge is very high, and loach is a kind of small-sized economic freshwater fish, is mainly distributed in East Asia Region, is of high nutritive value, have the title of ginseng waterborne.But the seed of China's loach breeding industry is mainly derived from wild seed, this is mainly very high due to lethality in loach fry large-scale cultivation process, and wild seed has the problem being difficult to tame and docile food artificial feed and survival rate instability.Therefore, the sound development of cultivation to loach industry of artificial seed is most important.The artificial propagation techniques of loach is mature on the whole; but because loach epidermal mucus is extremely many; extremely difficulty hand is caught; seriously reduce the efficiency of artificial induced spawning loach; but also often syringe can be caused to scratch parent because loach parent slides; cause mechanical damage and strongly stress, reduce spawning effect.Thus anaesthetic treatment must be carried out when loach parent hastens parturition.Fish anaesthetic has nearly 30 kinds more than, conventional has MS-222, eugenol, anaesthesine, that pyridine of sulfuric acid quinoline etc., the fish anesthesia of existing bibliographical information is band water anesthesia, as blueback salmon (Woodyetal., 2002), rainbow trout (Wagneretal., 2002), America hilsa herring (Du Hao etc., 2007), Cynoglossus semilaevis (Liu Changlin etc., 2008), mandarin fish (Guo Fenghong and Wang's sum, 2009), large yellow Crocker (Zhang Li and Wang's sum, 2010), the flat Rockfish of Xu Shi (key etc., 2010), verasper variegate (Zhao Ming etc., 2010) etc.But traditional band water anesthesia method is unsatisfactory to loach effect, practice shows that loach is difficult to reach anaesthetic effect under band water anesthesia, main cause should be that loach has intestinal respiration function, based on gas breathing in anesthesia liquid, anaesthetic is difficult to enter in loach body by the gill, then need very high anaesthetic concentration to be reached anaesthetic effect by Cutaneous permeation, band water anesthesia, for loach high cost, should not adopt.Thus the loach that the patent that publication No. is CN104082200A and CN104094878A provides is hastened parturition and hatching m does not adopt anesthesia, employs artificial fixing method.
Summary of the invention
The object of the invention is to customer service the deficiencies in the prior art, is the method that there are provided the efficient dry method anesthesia of a kind of loach parent, easy to implement the method, easy and simple to handle, is reached the effect of quick-anaesthesia, improve the efficiency of hastening parturition of loach significantly by Cutaneous permeation effect.
In order to realize above-mentioned object, the present invention adopts following technical measures:
The method that loach parent efficiently anaesthetizes, the steps include:
A, the loach parent chosen to be pulled out, in the plastic basin that is placed on and gets ready of draining the water;
B, anaesthetic to be dissolved in a small amount of clear water (10-30ml), after having dissolved, anaesthetic is poured in plastic basin;
C, loach parent reaction very acutely, can be added a cover a plastic basin and prevent loach parent from jumping out, anaesthetize after 3-5min above plastic basin;
D, start to inject oxytocic drug (LRH-A
220-25 μ g/kg; HCG2500-3000IU/kg; DOM5-8mg/kg), immediately loach parent is put into clear water after having injected, after loach parent revives completely in 5-10min, put into the equipment of laying eggs and wait to produce.
Gavaculine ethyl ester mesylate (3-Aminobenzoicacidethylestermethanesulfonate) selected by described anaesthetic, is commonly called as MS-222.
Described narcotic using dosage is 10mg/kg, is dissolved in 10-30ml clear water by anaesthetic during use.
Described oxytocic drug is luliberin analog 20-25 μ g/kg; Human chorionic gonadtropin 2500-3000IU/kg; Malaysia DOM 5-8mg/kg.
Compared with prior art, the invention has the beneficial effects as follows:
Dry method anaesthesia provided by the invention is very good to loach parent anaesthetic effect, significantly improves the efficiency of artificial induced spawning, avoid damage that conventional method causes loach parent, reduce that manual operation causes stress, improve spawning rate.
After dry method anesthesia, loach parent revives comparatively fast, all has no adverse effect to spawning rate, fertility rate and hatchability, is best suited for the method for loach parent anesthesia.Therefore the present invention proposes a kind of dry method anesthesia efficiently according to the respiratory characteristic of loach, the selection index system time is long, recovery is fast, the MS-222 of noresidue and toxic and side effect is as anaesthetic, reached the effect of quick-anaesthesia by Cutaneous permeation effect, improve the efficiency of hastening parturition of loach.
Find in practice, use dry method anesthesia can shorten inject time about 70%, parent's damage ratio reduces about 90%, and increased substantially efficiency, spawning rate can reach more than 90%, and fertility rate and hatchability all can reach more than 80%.
Embodiment
Embodiment 1: the technological means specifically setting forth the present invention's realization below in conjunction with example.
The method that loach parent efficiently anaesthetizes, the steps include:
A, May 12 days, choose each 5kg of loach parent male and female and prepare artificial induced spawning.Evening, 19:00 anaesthetized, and parent was pulled out, preanesthesia in the plastic basin that is placed on and gets ready of draining the water.
B, take 50mgMS-222 with analytical balance, be dissolved in 20ml clear water, pour into after having dissolved in plastic basin, cover a plastic basin immediately again, prevent loach parent from jumping out.
C, about 3 or 4 or about 5min, loach parent stops jumping completely, and anesthesia completes, and loach parental performance is now gill cover opening and closing, dehisces to swallow air, catches without significantly opposing, now start to inject oxytocic drug with hand.
D, injected after parent insert immediately in clear water, now loach rollover sink to the bottom, rarely seen opercular movement, about about 8min revives, stop rollover, start to move about.
Morning May 13 about 5:00, loach parent starts to lay eggs, and about 11:00 completed and laid eggs noon, check find spawning rate be 92.2%.
Embodiment 2:
After expanding the scale of production, applicant devises a kind of fixed operating desk, is convenient to carry out scale loach parent anesthesia, respond well.
The method that loach parent efficiently anaesthetizes, the steps include:
The fixed operating desk of A is a common cement table top, height 70-80cm, long 110-120cm, wide 60-80cm.Table top is placed the square plastic magazine of a long 100cm, wide 50cm, dark 30cm, for placing loach parent.
B chooses 50kg loach parent, is positioned in plastic basin, preanesthesia after draining the water.
C takes 500mgMS-222, is dissolved in 30ml clear water and anaesthetizes, and anesthesia is identical with embodiment 1, and the injection after anesthesia, parent's process etc. are all identical with embodiment 1
D uses fixed operating desk to be convenient to scale, standardized production operation, and the height of operating desk is according to the design of workman's normal sitting position height, and so, workman can carry out anaesthetizing very easily and parent injects work, has saved labour, has improve production efficiency.
Claims (1)
1. a loach parent method of efficiently anaesthetizing, the steps include:
A, the loach parent of selection to be pulled out, in the plastic basin that is placed on and gets ready of draining the water;
B, anaesthetic is dissolved in clear water, after dissolving, anaesthetic is poured in plastic basin;
C, loach parent react violent, add a cover a plastic basin and prevent loach parent from jumping out, anaesthetized after 3-5min above plastic basin;
D, start to inject oxytocic drug, after having injected, loach parent is put into clear water, after loach parent revives completely in 5-10min, put into the equipment of laying eggs and wait to produce;
Gavaculine ethyl ester mesylate selected by described anaesthetic;
Described narcotic using dosage is 10mg/kg, is dissolved in by anaesthetic in 10-30ml clear water during use;
Described oxytocic drug is luliberin analog 20-25 μ g/kg; Human chorionic gonadtropin 2500-3000IU/kg; Malaysia DOM 5-8mg/kg.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510700202.1A CN105165692B (en) | 2015-10-26 | 2015-10-26 | A kind of method of loach parent anesthesia |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510700202.1A CN105165692B (en) | 2015-10-26 | 2015-10-26 | A kind of method of loach parent anesthesia |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105165692A true CN105165692A (en) | 2015-12-23 |
| CN105165692B CN105165692B (en) | 2018-06-15 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510700202.1A Expired - Fee Related CN105165692B (en) | 2015-10-26 | 2015-10-26 | A kind of method of loach parent anesthesia |
Country Status (1)
| Country | Link |
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| CN (1) | CN105165692B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109699530A (en) * | 2019-01-25 | 2019-05-03 | 沧州北通达海洋工程机械科技有限公司 | Loach breeding induced spawning method |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08131014A (en) * | 1994-11-11 | 1996-05-28 | Masao Narita | Preparation of fertilized egg of loach |
| CN102217560A (en) * | 2010-04-16 | 2011-10-19 | 淮海工学院 | Loach fry artificial breeding method |
| CN103478023A (en) * | 2013-08-15 | 2014-01-01 | 江苏畜牧兽医职业技术学院 | Artificial propagation technology for Rhodeus sinensis |
| CN103843711A (en) * | 2014-04-03 | 2014-06-11 | 山东东方海洋科技股份有限公司 | Conveying method for trout |
| CN103947587A (en) * | 2014-04-15 | 2014-07-30 | 新疆维吾尔自治区水产科学研究所 | Anesthesia method for northern pike |
| CN104904630A (en) * | 2015-05-15 | 2015-09-16 | 新疆维吾尔自治区水产科学研究所 | Aspiorhynchuslaticeps anesthesia method and application thereof |
-
2015
- 2015-10-26 CN CN201510700202.1A patent/CN105165692B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08131014A (en) * | 1994-11-11 | 1996-05-28 | Masao Narita | Preparation of fertilized egg of loach |
| CN102217560A (en) * | 2010-04-16 | 2011-10-19 | 淮海工学院 | Loach fry artificial breeding method |
| CN103478023A (en) * | 2013-08-15 | 2014-01-01 | 江苏畜牧兽医职业技术学院 | Artificial propagation technology for Rhodeus sinensis |
| CN103843711A (en) * | 2014-04-03 | 2014-06-11 | 山东东方海洋科技股份有限公司 | Conveying method for trout |
| CN103947587A (en) * | 2014-04-15 | 2014-07-30 | 新疆维吾尔自治区水产科学研究所 | Anesthesia method for northern pike |
| CN104904630A (en) * | 2015-05-15 | 2015-09-16 | 新疆维吾尔自治区水产科学研究所 | Aspiorhynchuslaticeps anesthesia method and application thereof |
Non-Patent Citations (4)
| Title |
|---|
| 李倩等: ""台湾泥鳅"人工繁殖试验", 《河北渔业》 * |
| 杨宝瑞: "《辽宁省地方渔业标准汇编》", 30 April 2012, 辽宁科学技术出版社 * |
| 程敬华等: "泥鳅苗种繁育前期五注意", 《河南农业》 * |
| 韩光明等: "间氨基苯甲酸乙酯甲磺酸盐对泥鳅的麻醉效果", 《广东海洋大学学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109699530A (en) * | 2019-01-25 | 2019-05-03 | 沧州北通达海洋工程机械科技有限公司 | Loach breeding induced spawning method |
| CN109699530B (en) * | 2019-01-25 | 2021-09-07 | 佛山市嘉沃农业科技合伙企业（有限合伙） | Artificial spawning induction method for loach culture |
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| Publication number | Publication date |
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| CN105165692B (en) | 2018-06-15 |
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Inventor after: Fan Qixue Inventor after: Hu Weihua Inventor after: Zhang Yunlong Inventor after: Wu Qiaowan Inventor after: Wang Fan Inventor after: Yang Ruibin Inventor before: Fan Qixue Inventor before: Zhang Yunlong Inventor before: Wu Qiaowan Inventor before: Hu Weihua Inventor before: Wang Fan Inventor before: Yang Ruibin |
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