CN104904630A - Aspiorhynchuslaticeps anesthesia method and application thereof - Google Patents
Aspiorhynchuslaticeps anesthesia method and application thereof Download PDFInfo
- Publication number
- CN104904630A CN104904630A CN201510247848.9A CN201510247848A CN104904630A CN 104904630 A CN104904630 A CN 104904630A CN 201510247848 A CN201510247848 A CN 201510247848A CN 104904630 A CN104904630 A CN 104904630A
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- Prior art keywords
- anesthesia
- fish
- flat
- water
- aspiorhynchuslaticeps
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- 206010002091 Anaesthesia Diseases 0.000 title claims abstract description 55
- 230000037005 anaesthesia Effects 0.000 title claims abstract description 55
- 241001258470 Aspiorhynchus laticeps Species 0.000 title abstract description 10
- 238000000034 method Methods 0.000 title abstract description 9
- 241000251468 Actinopterygii Species 0.000 claims abstract description 65
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 35
- 230000003444 anaesthetic effect Effects 0.000 claims abstract description 18
- QCDWFXQBSFUVSP-UHFFFAOYSA-N 2-phenoxyethanol Chemical compound OCCOC1=CC=CC=C1 QCDWFXQBSFUVSP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 229960005323 phenoxyethanol Drugs 0.000 claims abstract description 11
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 4
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 4
- 239000001301 oxygen Substances 0.000 claims abstract description 4
- 102000002322 Egg Proteins Human genes 0.000 claims description 5
- 108010000912 Egg Proteins Proteins 0.000 claims description 5
- 210000004681 ovum Anatomy 0.000 claims description 5
- 230000009027 insemination Effects 0.000 claims description 3
- 230000003533 narcotic effect Effects 0.000 claims description 3
- 210000000582 semen Anatomy 0.000 abstract description 2
- 238000009395 breeding Methods 0.000 abstract 2
- 230000001488 breeding effect Effects 0.000 abstract 2
- 235000013601 eggs Nutrition 0.000 abstract 2
- 238000005516 engineering process Methods 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 238000001949 anaesthesia Methods 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- RRAFCDWBNXTKKO-UHFFFAOYSA-N eugenol Chemical compound COC1=CC(CC=C)=CC=C1O RRAFCDWBNXTKKO-UHFFFAOYSA-N 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 230000032258 transport Effects 0.000 description 2
- NPBVQXIMTZKSBA-UHFFFAOYSA-N Chavibetol Natural products COC1=CC=C(CC=C)C=C1O NPBVQXIMTZKSBA-UHFFFAOYSA-N 0.000 description 1
- 241001443588 Cottus gobio Species 0.000 description 1
- 241000252206 Cypriniformes Species 0.000 description 1
- 239000005770 Eugenol Substances 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- UVMRYBDEERADNV-UHFFFAOYSA-N Pseudoeugenol Natural products COC1=CC(C(C)=C)=CC=C1O UVMRYBDEERADNV-UHFFFAOYSA-N 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 239000011013 aquamarine Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 229960002217 eugenol Drugs 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000032696 parturition Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/075—Ethers or acetals
- A61K31/085—Ethers or acetals having an ether linkage to aromatic ring nuclear carbon
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- Public Health (AREA)
- Marine Sciences & Fisheries (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Epidemiology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Chemical & Material Sciences (AREA)
- Farming Of Fish And Shellfish (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses an aspiorhynchuslaticeps anesthesia method. The method comprises the steps that 1, clear water is added into an anesthetic container, wherein the concentration of dissolved oxygen is larger than or equal to 7 mg/L, and the temperature of water is the same as that of a water body where aspiorhynchuslaticeps is located; 2, 2-phenoxyethanol anesthetic is added into the clean water, which is obtained in the first step, in the anesthetic container and mixed evenly to obtain an anesthetic water source; 3, the aspiorhynchuslaticeps is placed in the anesthetic water source. The invention further provides application of the aspiorhynchuslaticeps anesthesia method in aspiorhynchuslaticeps artificial breeding. The aspiorhynchuslaticeps anesthesia method has the advantages that excessive anesthesia can be effectively avoided; the operating time for egg collection and semen collection of parent fishes after the parent fishes leave water in the aspiorhynchuslaticeps artificial breeding process is provided, the parent fishes can be used repeatedly, the collection scale of fertilized eggs and the utilization rate of parent fish resources are increased, the dependency on natural resources and the fishing intensity of the natural resources are lowered, and aspiorhynchuslaticeps resources can be sustainably developed and utilized.
Description
Technical field
The present invention relates to aquaculture field, be specifically related to anesthesia and the application thereof of a kind of flat kiss fish.
Background technology
Flat kiss fish Aspiorhynchus laticeps (Day) has another name called Xinjiang bullhead, belongs to Cypriniformes, Schizothoracinae, and flat kiss fish belongs to.This fish is the significant species in the fauna of Tarim Basin, is only distributed in Tarim River System, and full century, only this genus was a kind of, is also plant fish rare single genus in fish classification.Flat kiss fish was once distributed widely in the ground such as Bosten, Lop Nur of Tarim River System.According to historical summary, this fish was the important economic fish of Bosten originally, and maximum individuality can reach more than 40kg, and before the sixties in 20th century, maximum output can reach 200 tons, and annual production accounts for 10 ~ 20% of Bosten fish crop.Afterwards because of construction, the reason such as human economic development and variation of ecology and environment of river construction, cause Natural Population quantity sharply to decline, disappear from Bosten in late 1970s.Within 1988, this fish was listed in first-grade state protection aqua-marine life, and was embodied in " Chinese animals on the brink of extinction Red Data Book " in 1998, and threatened level is (E) in imminent danger.
Flat kiss fish adult is comparatively large, gives first aid to, transports, hastens parturition, adopts the operating process such as ovum and easily produce stress reaction even major injuries in enforcement.But also reported the anaesthesia technology about flat kiss fish without any scientific and technical literature so far.
Main anaesthetic in current application fishery has MS-222, eugenol etc., is widely used in, in the operations such as the transport of fish, sorting and vaccine inoculation, reducing the injury to fish body, improves survival rate.
Summary of the invention
Object of the present invention is exactly for above-mentioned defect of the prior art, provides a kind of anaesthesia technology by the short time, avoids the violent struggle of flat kiss fish in operating process, has increased substantially the anesthesia of the flat kiss fish of flat kiss fish survival rate.
To achieve these goals, technical scheme provided by the invention is: the anesthesia of a kind of flat kiss fish, comprises the following steps:
1) in anesthesia container, add clear water, dissolved oxygen is more than or equal to 7mg/L, and water temperature is identical with the flat water body kissing fish place;
2) add 2-Phenoxyethanol anaesthetic in the clear water of the anesthesia container obtained in step 1), obtain after mixing anaesthetizing water source;
3) flat kiss fish is positioned in anesthesia water source.
Further, the anesthesia of above-mentioned a kind of flat kiss fish, in described step 1), anesthesia container is the plastic box of inner wall smooth, and the required water depth of anesthesia is 30-40cm; Anesthesia containers size is long 80-100cm, wide 40-60cm.
Further, the anesthesia of above-mentioned a kind of flat kiss fish, described step 2) in, anaesthetic to be mass percent be 99.0% 2-Phenoxyethanol; Described narcotic additional proportion is according to volume ratio water: anaesthetic is 1000:(0.1-0.7).
Further, the anesthesia of above-mentioned a kind of flat kiss fish, in described step 3), anesthesia duration is 3min, and anesthesia water temperature is 12-21 DEG C.
Further, the anesthesia of above-mentioned a kind of flat kiss fish, in described step 3), after flat kiss fish parent fish anesthesia, the dried up time is less than 8min, and flat kiss fish returns to normal travelling state needs 3-5min.
Second object of the present invention there is provided the application of anesthesia in the artificial propagation of flat kiss fish of above-mentioned a kind of flat kiss fish.
Further, above-mentioned application, the artificial propagation of described flat kiss fish refers to flat kiss fish ovum artificial collection or artificial insemination.
Beneficial effect of the present invention is: the anesthesia of a kind of flat kiss fish provided by the invention, the anaesthetic 2-Phenoxyethanol used has cheap, fish body safety is wide with dead border, and solution bactericidal effect is good, can reduce the features such as ammonia N-excretion rates in fish anesthesia transportation.The present invention is by optimizing the relation of the concentration of 2-Phenoxyethanol, anesthesia duration, anesthesia water temperature and flat kiss fish anaesthetic effect, effectively can avoid the generation of excessively anaesthetizing phenomenon, and after parent population safety in the artificial propagation of flat kiss fish can be provided dried up, adopt the time of ovum and semen collection operation, make parent population by after reinforced cultivating, next year reusing, improve the collection scale of fertilized egg and the availability of parent management, reduce the dependence to natural resources and catching intensity, reach the object of flat kiss fish resource Sustainable Development and Utilization.
Embodiment
anaesthetic is originated:
2-Phenoxyethanol: buy from Wuhan Xin great Di environment-friendly materials Co., Ltd, trade name is " BASF Phenoxyethanol Protectol PE ".
embodiment 1:
An anesthesia for flat kiss fish, comprises the following steps:
1) in anesthesia container, add clear water, dissolved oxygen is more than or equal to 7mg/L, and water temperature is identical with the flat water body kissing fish place; Anesthesia container is the plastic box of inner wall smooth, and the required water depth of anesthesia is 30-40cm; Anesthesia containers size is long 80-100cm, wide 40-60cm;
2) add 2-Phenoxyethanol anaesthetic in the clear water of the anesthesia container obtained in step 1), obtain after mixing anaesthetizing water source; Anaesthetic to be mass percent be 99.0% 2-Phenoxyethanol; Described narcotic additional proportion is according to volume ratio water: anaesthetic is 1000:(0.1-0.7);
3) be positioned over by flat kiss fish in anesthesia water source, anesthesia duration is 3min, and anesthesia water temperature is 12-21 DEG C; After flat kiss fish parent fish anesthesia, the dried up time is less than 8min, and flat kiss fish returns to normal travelling state needs 3-5min.
The anesthesia of above-mentioned flat kiss fish can be applicable in the artificial propagation of flat kiss fish, is specially and can be applicable in flat kiss fish ovum artificial collection or artificial insemination.
Last it is noted that the foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although with reference to previous embodiment to invention has been detailed description, for a person skilled in the art, it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein portion of techniques feature.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (7)
1. an anesthesia for flat kiss fish, is characterized in that, comprise the following steps:
1) in anesthesia container, add clear water, dissolved oxygen is more than or equal to 7mg/L, and water temperature is identical with the flat water body kissing fish place;
2) add 2-Phenoxyethanol anaesthetic in the clear water of the anesthesia container obtained in step 1), obtain after mixing anaesthetizing water source;
3) flat kiss fish is positioned in anesthesia water source.
2. the anesthesia of a kind of flat kiss fish according to claim 1, it is characterized in that, in described step 1), anesthesia container is the plastic box of inner wall smooth, and the required water depth of anesthesia is 30-40cm; Anesthesia containers size is long 80-100cm, wide 40-60cm.
3. the anesthesia of a kind of flat kiss fish according to claim 2, is characterized in that, described step 2) in, anaesthetic to be mass percent be 99.0% 2-Phenoxyethanol; Described narcotic additional proportion is according to volume ratio water: anaesthetic is 1000:(0.1-0.7).
4. the anesthesia of a kind of flat kiss fish according to claim 3, it is characterized in that, in described step 3), anesthesia duration is 3min, and anesthesia water temperature is 12-21 DEG C.
5. the anesthesia of a kind of flat kiss fish according to claim 4, is characterized in that, in described step 3), after flat kiss fish parent fish anesthesia, the dried up time is less than 8min, and flat kiss fish returns to normal travelling state needs 3-5min.
6. according to the application of anesthesia in the artificial propagation of flat kiss fish of the arbitrary described a kind of flat kiss fish of claim 1-5.
7. apply according to claim 6, it is characterized in that, the artificial propagation of described flat kiss fish refers to flat kiss fish ovum artificial collection or artificial insemination.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510247848.9A CN104904630A (en) | 2015-05-15 | 2015-05-15 | Aspiorhynchuslaticeps anesthesia method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510247848.9A CN104904630A (en) | 2015-05-15 | 2015-05-15 | Aspiorhynchuslaticeps anesthesia method and application thereof |
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| Publication Number | Publication Date |
|---|---|
| CN104904630A true CN104904630A (en) | 2015-09-16 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN201510247848.9A Pending CN104904630A (en) | 2015-05-15 | 2015-05-15 | Aspiorhynchuslaticeps anesthesia method and application thereof |
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| Country | Link |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105165692A (en) * | 2015-10-26 | 2015-12-23 | 华中农业大学 | Method for anesthetizing loach parent |
Citations (4)
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| CN101238801A (en) * | 2008-03-05 | 2008-08-13 | 中国水产科学研究院淡水渔业研究中心 | Anaesthesia method of tilapia |
| CN101259167A (en) * | 2008-04-14 | 2008-09-10 | 中国水产科学研究院淡水渔业研究中心 | Carp anaesthesia method |
| CN102715115A (en) * | 2012-07-09 | 2012-10-10 | 中国水产科学研究院黑龙江水产研究所 | Phoxinus lagowskii fry breeding method |
| CN103947587A (en) * | 2014-04-15 | 2014-07-30 | 新疆维吾尔自治区水产科学研究所 | Anesthesia method for northern pike |
-
2015
- 2015-05-15 CN CN201510247848.9A patent/CN104904630A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101238801A (en) * | 2008-03-05 | 2008-08-13 | 中国水产科学研究院淡水渔业研究中心 | Anaesthesia method of tilapia |
| CN101259167A (en) * | 2008-04-14 | 2008-09-10 | 中国水产科学研究院淡水渔业研究中心 | Carp anaesthesia method |
| CN102715115A (en) * | 2012-07-09 | 2012-10-10 | 中国水产科学研究院黑龙江水产研究所 | Phoxinus lagowskii fry breeding method |
| CN103947587A (en) * | 2014-04-15 | 2014-07-30 | 新疆维吾尔自治区水产科学研究所 | Anesthesia method for northern pike |
Non-Patent Citations (2)
| Title |
|---|
| 关健等: "2-苯氧乙醇和丁香酚对许氏平鲉幼鱼麻醉效果的实验研究", 《渔业科学进展》 * |
| 张人铭等: "扁吻鱼人工繁殖技术研究", 《水生态学杂志》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105165692A (en) * | 2015-10-26 | 2015-12-23 | 华中农业大学 | Method for anesthetizing loach parent |
| CN105165692B (en) * | 2015-10-26 | 2018-06-15 | 华中农业大学 | A kind of method of loach parent anesthesia |
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Application publication date: 20150916 |
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| RJ01 | Rejection of invention patent application after publication |