CN104919937B - It is a kind of to promote the processing method that masson pine seed is germinateed after storage - Google Patents
It is a kind of to promote the processing method that masson pine seed is germinateed after storage Download PDFInfo
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- CN104919937B CN104919937B CN201510320334.1A CN201510320334A CN104919937B CN 104919937 B CN104919937 B CN 104919937B CN 201510320334 A CN201510320334 A CN 201510320334A CN 104919937 B CN104919937 B CN 104919937B
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Abstract
Promote the processing method that masson pine seed is germinateed after storage the invention discloses a kind of, after the pure masson pine seed sterilization after storage, be placed in the PEG6000 aqueous solution that mass fraction is 5% and soak 12 22 hours, cleaned with distilled water, be placed in 20mmolL‑1 Ca2+Solution and 50mgL‑1 GA3Soak 2 12 hours and take out in the mixed liquor of solution.By the masson pine seed germination percentage of the treated storage 2 years of the present invention up to 72.8% 79.3%, germinating energy is up to 47.5% 53.6%, germination index is up to 19.3 21.4, and this method is easy to operate, inexpensive, time saving and energy saving, masson pine seed has neat germination, germination percentage height, the characteristics of fast, seedling quality is high of emerging after processing, masson pine seed utilization rate, tree seedling emergence rate and seedling quality can be increased substantially, and storage technique and the reduction cost of storage can be simplified.
Description
Technical field
The invention belongs to plant seed germination seedling-raising technique field, it is related to what masson pine seed after a kind of promotion storage was germinateed
Processing method.
Background technology
Masson pine (Pinus massoniana Lamb.) also known as fir, pine, pachydermia pine etc., because its strong adaptability,
Fast-growing, high yield, full tree comprehensive utilization degree are high, and fiber is excellent and turns into the Major Tree Species Planted that south Shi Si is saved, and is providing pine
Occupy highly important status in terms of fat and paper making raw material, Building wood.
Storage for the winter is typically passed through before being sowed after masson pine seed collection, it is that masson pine seed is economical and practical that normal temperature, which does Tibetan,
Storage practice, using existing National Standards Technical handle masson pine seed, with 45 DEG C begin temperature immersion seed 24h, its germinate
Index is shown in Table 1.Because of masson pine seed, easily aging is inactivated under the conditions of the dry Tibetan of normal temperature, cell membrane function and structural damage, enzyme activity
Property reduction, cause seed vitality reduction, germinating capacity and seedling quality reduction, influence afforestation survive and quality, therefore, existing skill
The method effect that masson pine seed germination percentage is improved in art is all undesirable.Research shows that masson pine seed is housed in -5 DEG C ~ 5 DEG C
Left and right can keep higher germination percentage level, and the moisture content of the storage of seeds is preferably 5% ~ 10%, is required in the storage technique
Seed moisture content and temperature are whard to control, and the storage of seeds power consumption is big, high cost.Therefore, it is difficult to ensure that masson pine seed in production
It can be preserved under preferable holding conditions, and then ensure the vigor of seed after storage.
In existing literature " influence of PEG Pretreatment on Seed Vigor of Masson Pine "（Analogy circumference etc., Nanjing forestry
College journal, 2000,24 (1): 38-40）Described in handle aging masson pine seed 3 days with 10%PEG10000, it germinates
Rate, germination index and vitality index respectively than compare the processing of existing international standard technical it is high by 34.29%, 24.59%, 13.0%, it is and literary
Offer " influence of calcium soaking on Germination of Pinus massoniana Seed "（Wei little Li etc., seed, 2005,24 (4): 34-36）Described in use
20mmol·L-1Ca2+Masson pine seed 24h is handled, its germination percentage, germination index, vitality index are respectively than compareing existing state
Border standard technique processing improves 10.91%, 10.14%, 52.38%.The germination effect of these processing is below at each initiation of the invention
The raising amount of the lower corresponding index of reason.How storage after masson pine seed vigor and germinating capacity is improved, to rationally effectively using horse
Tail pine seed, reduces seed and wastes significant.
The content of the invention
The technical problem to be solved in the present invention is：The processing method that masson pine seed is germinateed after a kind of promotion storage is provided,
The problem of masson pine seed vigor is low, germination percentage is low, germination regularity is low and storage technique requirement is high after storage is aimed to solve the problem that, with
The germination percentage and use value of aging masson pine seed are improved, seed is reduced and wastes, simplifies storage technique and reduces the cost of storage,
To overcome the shortcomings of prior art problem.
Technical scheme：It is a kind of promote storage after masson pine seed germinate processing method, this method include with
Lower step：
（1）By the pure masson pine seed after storage, sterilized 2-4 minutes using 0.3 ‰ mercuric chloride solution, while to germination
During the germinating apparatus, equipment and the apparatus that are related to disinfected accordingly；
（2）By step（1）Seed after middle sterilization, is placed in the PEG6000 aqueous solution that mass fraction is 5%, liquor capacity
It is advisable with being totally submerged seed, is sufficiently stirred for making after seed is totally submerged in solution, soaks 12-22 hours；
（3）By step（2）Seed after immersion is cleaned 2-3 times with distilled water, is placed in 20mmolL-1 Ca2+Solution and
50mg·L-1 GA3In the mixed liquor of solution, it is stirred well to seed and is totally submerged, soaks 2-12 hours；
（4）By step（3）Seed after middle processing takes out from soak, is cleaned 2-3 times with distilled water.
It is preferred that, above-mentioned steps（2）Middle soak time is 16 hours, the step（3）Middle soak time is 8 hours, should
Immersion way can reach 79.3% germination percentage, 53.6% germinating energy, and germinating power is significantly improved.
It is preferred that, above-mentioned steps（3）In Ca2+Solution is CaCl2Solution.
Beneficial effects of the present invention：There is following effect compared with prior art：
（1）The present invention use mass fraction for 5% the PEG6000 aqueous solution and 20mmolL-1 Ca2+Solution and 50mg
L-1 GA3The masson pine seed germination percentage of the mixed liquor processing storage 2 years of solution up to 72.8%-79.3%, than by《Tree seed
Inspection procedure》（GB2772-1999）Processing improves 67.4%-82.3%, and germinating energy is up to 47.5%-53.6%, than national standard side
Method processing improves 90.0%-114.4%, and germination index improves 116.6%- up to 19.3-21.4 than national standard method processing
139.9%；
（2）The medicine that the present invention is used easily is bought, easy to operate, inexpensive, time saving and energy saving, after present invention processing, horse
Tail pine seed has the characteristics of germination is neat, germination percentage is high, germination is fast, seedling quality is high, can improve masson pine seed utilization rate,
Improve masson pine tree seedling emergence rate and seedling quality；
（3）The present invention use mass fraction for 5% the PEG6000 aqueous solution and 20mmolL-1 Ca2+Solution and 50mg
L-1 GA3The mixed liquor processing masson pine seed of solution, can be substantially reduced in storage technique to reserve temperature and seed moisture content
High request, simplify storage facilities, substantially reduce the cost of storage, expand the scope of the storage of seeds.
For further instruction beneficial effects of the present invention, a few class solution ratios processing experiment shown in table 2 has been carried out,
No. VII in table（CK）Using the experiment of national standard method, to obtain the different germination indexs for triggering and handling lower masson pine seed（Table 3）.
As shown in Table 3, the PEG6000 aqueous solution and 20mmolL by mass fraction for 5%-1 Ca2+Solution and 50mgL-1 GA3
The mixed liquor processing masson pine seed of solution, handles 24 hours total times, by contrast, its germinating power index is substantially better than in addition
Several classes.
Embodiment
The present invention is further explained in conjunction with following example.
Embodiment 1：It is a kind of to promote the processing method that masson pine seed is germinateed after storage, comprise the following steps：
（1）By the pure masson pine seed after storage, sterilized 2-4 minutes using 0.3 ‰ mercuric chloride solution, while to germination
During the germinating apparatus, equipment and the apparatus that are related to disinfected accordingly；
（2）The seed after sterilization is placed in the PEG6000 aqueous solution that mass fraction is 5%, liquor capacity to soak completely
Do not have seed to be advisable, be sufficiently stirred for making after seed is totally submerged in solution, soak 22 hours；
（3）By step（2）Seed after immersion is cleaned 2-3 times with distilled water, is placed in 20mmolL-1 Ca2+Solution and
50mg·L-1 GA3In the mixed liquor of solution, it is stirred well to seed and is totally submerged, soaks 2 hours；
（4）Will processing（3）Seed after middle processing takes out from soak, is cleaned 2-3 times with distilled water.
The masson pine seed germination percentage of the storage 2 years treated by the methods described of embodiment 1 up to 72.8%, than by《Woods
Wooden Seed Inspection code》（GB2772-1999）Processing improves 67.4%, and germinating energy is improved up to 52.0% than national standard processing
108.0%, germination index improves 116.6% up to 19.32 than national standard processing.
Embodiment 2：It is a kind of to promote the processing method that masson pine seed is germinateed after storage, comprise the following steps：
（1）By the pure masson pine seed after storage, sterilized 2-4 minutes using 0.3 ‰ mercuric chloride solution, while to germination
During the germinating apparatus, equipment and the apparatus that are related to disinfected accordingly；
（2）The seed after sterilization is placed in the PEG6000 aqueous solution that mass fraction is 5%, liquor capacity to soak completely
Do not have seed to be advisable, be sufficiently stirred for making after seed is totally submerged in solution, soak 12 hours；
（3）By step（2）Seed after immersion is cleaned 2-3 times with distilled water, is placed in 20mmolL-1 Ca2+Solution and
50mg·L-1 GA3In the mixed liquor of solution, Ca2+Solution is CaCl2Solution, is stirred well to seed and is totally submerged, and immersion 12 is small
When；
（4）By step（3）Seed after middle processing takes out from soak, is cleaned 2-3 times with distilled water.
The masson pine seed germination percentage of the storage 2 years treated by the methods described of embodiment 2 up to 75.8%, than by
《Tree Seeds Test code》（GB2772-1999）Processing improves 74.3%, and germinating energy is improved up to 47.5% than national standard
90.0%, germination index improves 125.1% up to 20.1 than national standard processing.
Embodiment 3：It is a kind of to promote the processing method that masson pine seed is germinateed after storage, comprise the following steps：
（1）By the pure masson pine seed after storage, sterilized 2-4 minutes using 0.3 ‰ mercuric chloride solution, while to germination
During the germinating apparatus, equipment and the apparatus that are related to disinfected accordingly；
（2）The seed after sterilization is placed in the PEG6000 aqueous solution that mass fraction is 5%, liquor capacity to soak completely
Do not have seed to be advisable, be sufficiently stirred for making after seed is totally submerged in solution, soak 16 hours；
（3）By step（2）Seed after immersion is cleaned 2-3 times with distilled water, is placed in 20mmolL-1 Ca2+Solution and
50mg·L-1 GA3In the mixed liquor of solution, Ca2+Solution is CaCl2Solution, is stirred well to seed and is totally submerged, and immersion 8 is small
When；
（4）By step（3）Seed after middle processing takes out from soak, is cleaned 2-3 times with distilled water.
The masson pine seed germination percentage of the storage 2 years treated by the methods described of embodiment 3 up to 79.3%, than by
《Tree Seeds Test code》（GB2772-1999）Processing improves 82.3%, and germinating energy is improved up to 53.6% than national standard
114.4%, germination index improves 139.9% up to 21.4 than national standard processing.
Claims (2)
1. a kind of promote the processing method that masson pine seed is germinateed after storage, it is characterised in that：This method comprises the following steps：
（1）Sterilize 2-4 minutes, the pure masson pine seed after storage while to germination process using 0.3 ‰ mercuric chloride solution
In the germinating apparatus, equipment and the apparatus that are related to disinfected accordingly；
（2）By step（1）Seed after middle sterilization, is placed in the PEG6000 aqueous solution that mass fraction is 5%, liquor capacity is with complete
Full submergence seed is advisable, and is sufficiently stirred for making after seed is totally submerged in solution, soaks 12-22 hours；
（3）By step（2）Seed after immersion is cleaned 2-3 times with distilled water, is placed in 20mmolL-1 Ca2+Solution and 50mg
L-1In the mixed liquor of GA3 solution, it is stirred well to seed and is totally submerged, soaks 2-12 hours；
（4）By step（3）Seed after middle processing takes out from soak, is cleaned 2-3 times with distilled water；
The step（2）Middle soak time is 16 hours, the step（3）Middle soak time is 8 hours.
2. the processing method that masson pine seed is germinateed after a kind of promotion storage as claimed in claim 1, it is characterised in that：It is described
Step（3）In Ca2+Solution is CaCl2Solution.
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| CN201510320334.1A CN104919937B (en) | 2015-06-12 | 2015-06-12 | It is a kind of to promote the processing method that masson pine seed is germinateed after storage |
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| AU2004252479A1 (en) * | 2003-06-06 | 2005-01-06 | Arborgen Llc. | Transcription factors |
| CN102124836B (en) * | 2011-01-30 | 2012-07-04 | 广西壮族自治区林业科学研究院 | Method for processing strobili of pinus massoniana |
| CN102792804B (en) * | 2012-08-31 | 2014-07-30 | 广西壮族自治区林业科学研究院 | Chinese red pine seed storing method |
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