CN104919937A - Treating method for promoting germination of stored pinus massoniana Lamb. seeds - Google Patents
Treating method for promoting germination of stored pinus massoniana Lamb. seeds Download PDFInfo
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- CN104919937A CN104919937A CN201510320334.1A CN201510320334A CN104919937A CN 104919937 A CN104919937 A CN 104919937A CN 201510320334 A CN201510320334 A CN 201510320334A CN 104919937 A CN104919937 A CN 104919937A
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Abstract
The invention discloses a treating method for promoting germination of stored pinus massoniana Lamb. seeds. The treating method comprises the following steps: sterilizing the stored pinus massoniana Lamb. seeds, placing the sterilized seeds into a PEG6000 water solution with the mass fraction of 5% to be soaked for 12-22 h, cleaning with distilled water, placing the cleaned seeds into a mixed solution of 20 mmol.L<-1> Ca<2+> solution and a 50 mg.L<-1> GA3 solution to be soaked for 2-12 h and then taking out. The germination rate of the pinus massoniana Lamb. seeds stored for two years and treated by the treating method can reach 72.8-79.3%, the germination potential can reach 47.5-53.6%, the germination index can reach 19.3-21.4, the method is easy to operate and low in cost, and saves time and labor, the treated pinus massoniana Lamb. seeds have the characteristics of being ordered in germination, high in germination rate, quick in seedling emergence and high in seedling quality, the use ratio of the pinus massoniana Lamb. seeds, the seedling emergence rate of sown seedlings and the quality of the seedlings can greatly improved, the storing technology can be simplified and the storing cost can be reduced.
Description
Technical field
The invention belongs to plant seed germination seedling growing process field, relate to a kind of processing method promoting to preserve the germination of rear masson pine seed.
Background technology
Masson pine (
pinus massonianalamb.) fir, pine, pachydermia pine etc. are had another name called, because of its strong adaptability, fast-growing, high yield, full tree comprehensive utilization degree is high, and fiber is excellent and become the Major Tree Species Planted that southern Shi Si economizes, and occupies very consequence providing in rosin and paper making raw material, Building wood.
Usually will through storage for the winter before sowing after masson pine seed collection, normal temperature is done and hidden is the economical and practical storage practice of masson pine seed, and adopt existing National Standards Technical process masson pine seed, soak seed 24h with 45 DEG C of temperature that begin, its germinate index is in table 1.Because of masson pine seed easy aging inactivation under normal temperature does Tibetan condition, cell membrane function and structural damage, enzymic activity reduce, seed vitality reduction, germinating capacity and seedling quality is caused to reduce, affect afforestation to survive and quality, therefore, the method effect improving masson pine seed germination rate in prior art is all undesirable.Research shows, masson pine seed is housed in-5 DEG C ~ about the 5 DEG C germination rate levels that can keep higher, and the moisture content of seed storage is preferably 5% ~ 10%, the seed moisture content required in this storage technique and temperature wayward, seed storage power consumption is large, and cost is high.Therefore, production is difficult to ensure that masson pine seed can be preserved under desirable holding conditions, and then ensures the vigor of the rear seed of storage.
At existing document " impact of PEG Pretreatment on Seed Vigor of Masson Pine " (analogy circumference etc., Nanjing Forestry University's journal, 2000,24 (1): 38-40) be described in and use 10%PEG10000 treatment of aged masson pine seed 3 days, its germination rate, germination index and vitality index respectively than contrast existing international standard technical process high by 34.29%, 24.59%, 13.0%, and document " impact of calcium soaking on Germination of Pinus massoniana Seed " (Wei little Li etc., seed, 2005,24 (4): 34-36) be described in and use 20mmolL
-1ca
2+process masson pine seed 24h, its germination rate, germination index, vitality index be international standard technical process improve 10.91%, 10.14%, 52.38% more existing than contrast respectively.The germination effect of these process all respectively causes the increasing amount of the lower corresponding index of process lower than the present invention.How improving the rear masson pine seed vigor of storage and germinating capacity, to rationally effectively using masson pine seed, reducing seed waste significant.
Summary of the invention
The technical problem to be solved in the present invention is: provide a kind of processing method promoting to preserve the germination of rear masson pine seed, be intended to solve the problem that after storage, masson pine seed vigor is low, germination rate is low, germination regularity is low and storage technique requirement is high, to improve germination rate and the use value of aging masson pine seed, reduce seed waste, simplify storage technique and reduce the cost of storage, to overcome the deficiency of prior art problem.
Technical scheme of the present invention: a kind of processing method promoting to preserve rear masson pine seed and germinate, the method comprises the following steps:
(1) by the pure masson pine seed after storage, use the mercuric chloride solution sterilization 2-4 minute of 0.3 ‰, the germinating apparatus related to, equipment and apparatus are disinfected accordingly simultaneously in germination process;
(2) by the seed after sterilization in step (1), be placed in the PEG6000 aqueous solution that mass fraction is 5%, liquor capacity is advisable with complete submergence seed, fully stirs and seed is immersed in after in solution completely, soak 12-22 hour;
(3) the seed distilled water cleaning after step (2) being soaked 2-3 time, is placed in 20mmolL
-1ca
2+solution and 50mgL
-1gA
3in the mixed liquor of solution, be stirred well to the complete submergence of seed, soak 2-12 hour;
(4) seed after process in step (3) is taken out from soak, with distilled water cleaning 2-3 time.
Preferably, in above-mentioned steps (2), soak time is 16 hours, and in described step (3), soak time is 8 hours, and this immersion way can reach the germination rate of 79.3%, the germination vigor of 53.6%, and germinating capacity significantly improves.
Preferably, the Ca in above-mentioned steps (3)
2+solution is CaCl
2solution.
Beneficial effect of the present invention: compared with prior art have following effect:
(1) the present invention adopt mass fraction be 5% the PEG6000 aqueous solution and 20mmolL
-1ca
2+solution and 50mgL
-1gA
3the masson pine seed germination rate that the mixed liquor process of solution is preserved 2 years can reach 72.8%-79.3%, 67.4%-82.3% is improved than by " Tree Seeds Test code " (GB2772-1999) process, germination vigor can reach 47.5%-53.6%, 90.0%-114.4% is improved than national standard method process, germination index can reach 19.3-21.4, improves 116.6%-139.9% than national standard method process;
(2) medicine that the present invention uses easily is bought, easy to operate, low cost, time saving and energy saving, after process of the present invention, masson pine seed has the feature neat, germination rate is high, germination is fast, seedling quality is high of germinateing, and can improve masson pine seed availability, improve masson pine tree seedling emergence rate and seedling quality;
(3) the present invention adopt mass fraction be 5% the PEG6000 aqueous solution and 20mmolL
-1ca
2+solution and 50mgL
-1gA
3the mixed liquor process masson pine seed of solution, can reduce the high request to reserve temperature and seed moisture content in storage technique greatly, simplifies storage facilities, greatly reduces the cost of storage, expands the scope of seed storage.
In order to further instruction beneficial effect of the present invention, carried out a few class solution ratio process tests shown in table 2, in table, No. VII (CK) is the test adopting national standard method, obtains the different germinate index (table 3) causing the lower masson pine seed of process.As shown in Table 3, by mass fraction be 5% the PEG6000 aqueous solution and 20mmolL
-1ca
2+solution and 50mgL
-1gA
3the mixed liquor process masson pine seed of solution, 24 hours total times of process, by contrast, its germinating capacity index is obviously better than other several classes.
Embodiment
Now in conjunction with following example, the present invention is further explained.
Embodiment 1: a kind of processing method promoting to preserve rear masson pine seed and germinate, comprises the steps:
(1) by the pure masson pine seed after storage, use the mercuric chloride solution sterilization 2-4 minute of 0.3 ‰, the germinating apparatus related to, equipment and apparatus are disinfected accordingly simultaneously in germination process;
(2) by the seed after sterilization, be placed in the PEG6000 aqueous solution that mass fraction is 5%, liquor capacity is advisable with complete submergence seed, fully stirs and seed is immersed in after in solution completely, soak 22 hours;
(3) the seed distilled water cleaning after step (2) being soaked 2-3 time, is placed in 20mmolL
-1ca
2+solution and 50mgL
-1gA
3in the mixed liquor of solution, be stirred well to the complete submergence of seed, soak 2 hours;
(4) seed processed in (3) after process is taken out from soak, with distilled water cleaning 2-3 time.
Can 72.8% be reached by the storage masson pine seed germination rate of 2 years of method process described in embodiment 1, raising 67.4% is processed than by " Tree Seeds Test code " (GB2772-1999), germination vigor can reach 52.0%, 108.0% is improved than national standard process, germination index can reach 19.32, improves 116.6% than national standard process.
Embodiment 2: a kind of processing method promoting to preserve rear masson pine seed and germinate, comprises the steps:
(1) by the pure masson pine seed after storage, use the mercuric chloride solution sterilization 2-4 minute of 0.3 ‰, the germinating apparatus related to, equipment and apparatus are disinfected accordingly simultaneously in germination process;
(2) by the seed after sterilization, be placed in the PEG6000 aqueous solution that mass fraction is 5%, liquor capacity is advisable with complete submergence seed, fully stirs and seed is immersed in after in solution completely, soak 12 hours;
(3) the seed distilled water cleaning after step (2) being soaked 2-3 time, is placed in 20mmolL
-1ca
2+solution and 50mgL
-1gA
3in the mixed liquor of solution, Ca
2+solution is CaCl
2solution, is stirred well to the complete submergence of seed, soaks 12 hours;
(4) seed after process in step (3) is taken out from soak, with distilled water cleaning 2-3 time.
Can 75.8% be reached by the storage masson pine seed germination rate of 2 years of method process described in embodiment 2, raising 74.3% is processed than by " Tree Seeds Test code " (GB2772-1999), germination vigor can reach 47.5%, 90.0% is improved than national standard, germination index can reach 20.1, improves 125.1% than national standard process.
Embodiment 3: a kind of processing method promoting to preserve rear masson pine seed and germinate, comprises the steps:
(1) by the pure masson pine seed after storage, use the mercuric chloride solution sterilization 2-4 minute of 0.3 ‰, the germinating apparatus related to, equipment and apparatus are disinfected accordingly simultaneously in germination process;
(2) by the seed after sterilization, be placed in the PEG6000 aqueous solution that mass fraction is 5%, liquor capacity is advisable with complete submergence seed, fully stirs and seed is immersed in after in solution completely, soak 16 hours;
(3) the seed distilled water cleaning after step (2) being soaked 2-3 time, is placed in 20mmolL
-1ca
2+solution and 50mgL
-1gA
3in the mixed liquor of solution, Ca
2+solution is CaCl
2solution, is stirred well to the complete submergence of seed, soaks 8 hours;
(4) seed after process in step (3) is taken out from soak, with distilled water cleaning 2-3 time.
Can 79.3% be reached by the storage masson pine seed germination rate of 2 years of method process described in embodiment 3, raising 82.3% is processed than by " Tree Seeds Test code " (GB2772-1999), germination vigor can reach 53.6%, 114.4% is improved than national standard, germination index can reach 21.4, improves 139.9% than national standard process.
Claims (3)
1. promote a processing method of preserving the germination of rear masson pine seed, it is characterized in that: the method comprises the following steps:
(1) by the pure masson pine seed after storage, use the mercuric chloride solution sterilization 2-4 minute of 0.3 ‰, the germinating apparatus related to, equipment and apparatus are disinfected accordingly simultaneously in germination process;
(2) by the seed after sterilization in step (1), be placed in the PEG6000 aqueous solution that mass fraction is 5%, liquor capacity is advisable with complete submergence seed, fully stirs and seed is immersed in after in solution completely, soak 12-22 hour;
(3) the seed distilled water cleaning after step (2) being soaked 2-3 time, is placed in 20mmolL
-1ca
2+solution and 50mgL
-1in the mixed liquor of GA3 solution, be stirred well to the complete submergence of seed, soak 2-12 hour;
(4) seed after process in step (3) is taken out from soak, with distilled water cleaning 2-3 time.
2. a kind of processing method promoting to preserve rear masson pine seed and germinate as claimed in claim 1, it is characterized in that: in described step (2), soak time is 16 hours, in described step (3), soak time is 8 hours.
3. a kind of processing method promoting to preserve rear masson pine seed and germinate as claimed in claim 1, is characterized in that: the Ca in described step (3)
2+solution is CaCl
2solution.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510320334.1A CN104919937B (en) | 2015-06-12 | 2015-06-12 | It is a kind of to promote the processing method that masson pine seed is germinateed after storage |
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|---|---|---|---|
| CN201510320334.1A CN104919937B (en) | 2015-06-12 | 2015-06-12 | It is a kind of to promote the processing method that masson pine seed is germinateed after storage |
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|---|---|
| CN104919937A true CN104919937A (en) | 2015-09-23 |
| CN104919937B CN104919937B (en) | 2017-07-11 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005001050A2 (en) * | 2003-06-06 | 2005-01-06 | Arborgen, Llc. | Transcription factors |
| CN102124836A (en) * | 2011-01-30 | 2011-07-20 | 广西壮族自治区林业科学研究院 | Method for processing strobili of pinus massoniana |
| CN102792804A (en) * | 2012-08-31 | 2012-11-28 | 广西壮族自治区林业科学研究院 | Chinese red pine seed storing method |
-
2015
- 2015-06-12 CN CN201510320334.1A patent/CN104919937B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005001050A2 (en) * | 2003-06-06 | 2005-01-06 | Arborgen, Llc. | Transcription factors |
| CN102124836A (en) * | 2011-01-30 | 2011-07-20 | 广西壮族自治区林业科学研究院 | Method for processing strobili of pinus massoniana |
| CN102792804A (en) * | 2012-08-31 | 2012-11-28 | 广西壮族自治区林业科学研究院 | Chinese red pine seed storing method |
Non-Patent Citations (2)
| Title |
|---|
| 吴明艳 等: "钙浸种对马尾松与重阳木种子发芽的影响", 《现代农业科技》 * |
| 喻方圆 等: "聚乙二醇渗透处理对马尾松种子活力的影响", 《南京林业大学学报》 * |
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| CN104919937B (en) | 2017-07-11 |
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Granted publication date: 20170711 |