CN104860733A - Microbial organic fertilizer capable of improving acidity of soil - Google Patents
Microbial organic fertilizer capable of improving acidity of soil Download PDFInfo
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- CN104860733A CN104860733A CN201510179533.5A CN201510179533A CN104860733A CN 104860733 A CN104860733 A CN 104860733A CN 201510179533 A CN201510179533 A CN 201510179533A CN 104860733 A CN104860733 A CN 104860733A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention discloses a microbial organic fertilizer capable of improving acidity of soil. Strains contained in the organic fertilizer disclosed by the invention can produce acid under different fermentation conditions, so that compared with inorganic salts, the organic fertilizer has the advantages that acid production is lasting, frequent applying is not required, the problems of soil caking and the like caused by anion enrichment do not occur, and meanwhile, a micro-ecological environment for root growth of alkaline soil crops can be optimized; the strain are derived from poor soil, i.e., corn rhizosphere soil, so that the tolerance of crops such as lactuca sativa, corn and the like to alkaline soil can be obviously promoted, the activity of urease of the soil can be improved, and an excellent liquid bacterial fertilizer can be provided for the growth of the crops such as lactuca sativa, corn and the like in the alkaline soil; and the organic fertilizer prepared from the strains can be used for obviously increasing the chlorophyll content of leaf tissue of the crops such as lactuca sativa, corn and the like, the Vc content of leaves of the crops such as lactuca sativa, corn and the like and the yield of the crops such as lactuca sativa, corn and the like, thereby having the good effect of improving quality and increasing yield.
Description
Technical field
The present invention relates to bio-organic fertilizer, particularly relate to
a kind of have the microbial bacteria fertilizer improving soil acidity.
Background technology
Bacillus amyloliquefaciens is a kind of bacterium very high with subtilis affinity, can produce a series of meta-bolites in the process of growth of himself, these meta-bolitess make bacillus amyloliquefaciens can have the activity of Antifungi and bacterium widely.Therefore, a large amount of scholar is doing a large amount of explorations to the new discovery of bacillus amyloliquefaciens and the optimization of new opplication and gene functional research and fermentation condition in recent years.Bacillus amyloliquefaciens can produce multiple antimicrobial substance, fungicidal spectrum is wide, to pathogenic bacteria, there is preventive and therapeutic effect, therefore it prevents and treats crop, vegetables, melon and fruit disease effect highly significant, market development prospect is huge, what is more important, it is for reducing chemical pesticide, ensureing that food safety provides an effective way.
I
state norththe regional alkaline soil in side is more, and owing to using chemical fertilizer in a large number throughout the year, " red frost ", " white alkali " appear in soil in a lot of booth again and again, and this is all the symptom of soil meta-alkali.Alkaline soil improving technology mainly comprises improvement soil, culture fertility, strengthen the affine performance of soil, the muck become thoroughly decomposed as applied, peat, wood sawdust, mugineic acid fertilizer, as lead sulfate, ferrous sulfate, SULPHUR POWDER, ammonium sulfate, ammonium nitrate, ammonium chloride, calcium superphosphate, potassium primary phosphate, potassium sulfate etc., directed neutralization alkalescence etc.Although above method improvement surface soil alkalinity instant effect, action time is not long, needs frequent repetitive administration.In conjunction with the biological fermentation of fertilizer, the solution starch gemma bar that acid is produced in screening makes bio-bacterial manure, by the Tiny ecosystem pH of fine setting rhizosphere soil, can solve alkaline soil crop root growth micro-ecological environment, thus promote healthy growth of crops.But the growing environment that bacillus amyloliquefaciens is suitable for is the nutrient solution of high protein and pH value is the environment of 3 ~ 5, grow comparatively slow in alkaline soils, so need to carry out in advance expanding be fitted on certain number and provide enough protein environment could improve its effect.
Summary of the invention
For the problems referred to above, the present invention aims to provide that a kind of microbial bacteria content is high and nutrition-allocated proportion is rationally beneficial to bacterial classification grows fast
?there is the microbial bacteria fertilizer improving soil acidity.
For realizing this technical purpose, the solution of the present invention is:
a kind of have the microbial bacteria fertilizer improving soil acidity, comprise a kind of bacillus amyloliquefaciens with acid producing ability,
The first step, is inoculated into bacillus amyloliquefaciens in nutrient solution, carries out liquid fermenting life, working condition is, pH value is between 3.8 ~ 4.2, temperature 20 ~ 35 DEG C, fermentation time obtains original fermented solution in 5 ~ 7 days, wherein stirring velocity is 120 ~ 300 revs/min, and in described nutrient solution 1L, substances content is: the potato juice of 80 ~ 120g/L, yeast powder 0.5 ~ 1.5g, molasses 3 ~ 8g, KH2PO4 0.01g, sodium-chlor 0.5 ~ 1.5g, K2HPO4 0.01g; Bacterial strain WSA061 is carried out enlarged culturing, joining original fermented solution in the product ferment liquid of new configuration according to total amount 1:4, the greenhouse being placed in 25 ~ 40 DEG C carry out enlarged culturing 3 ~ 4 days can obtain zymocyte liquid;
Second step, by the mixing microorganisms zymolyte of the pig manure become thoroughly decomposed and the dish dregs of rice in mass ratio 7: 3 ratio mix and obtain coarse fodder; Then the zymocyte liquid the first step prepared is inoculated in coarse fodder by the amount of 60 ~ 180L/ ton and carries out solid fermentation, control temperature is below 55 DEG C, ferment 6 ~ 9 days, carry out turning 1 ~ 2 times/day in fermenting process, wherein after fermentation ends, bacterial strain WSA061 content reaches 1.5 × 10
8more than cfu/g, obtains microbial organic fertilizer;
Finally, be no more than the condition of 55 DEG C in temperature under, the water content of microbial organic fertilizer is evaporated to less than 30%, pack of finally packing.
As preferably, the mixing microorganisms zymolyte preparation method of the described dish dregs of rice is: milk-acid bacteria, yeast, genus bacillus and Geotrichum are mixed according to 5:3:4:1, mixing microorganisms is added according to 1%--2% inoculum size in the dish dregs of rice, add the water of 55 ~ 65% of dish dregs of rice quality, obtain the mixing microorganisms zymolyte of the dish dregs of rice 15 ~ 20 DEG C of temperature bottom fermentation 48 hours fermentations.
As preferably, described in the pig manure germination index that becomes thoroughly decomposed be more than 98%, quality of organic matter is than content more than 45%, and organonitrogen quality is more than 1% than content, water content mass ratio 25-30%.
The microbial bacteria of the product acid function that the present invention relates to and prepare fertilizer there is following beneficial effect:
1. the bacterial classification of this fertilizer is a kind of bacillus amyloliquefaciens of fermentation and acid function, all acid can be produced under different fermentation conditions, relative to inorganic salt, produce acid lasting, without the need to frequent applying, and the problem such as the soil block that there will not be negatively charged ion enrichment and produce, alkaline soil crop root growth micro-ecological environment can be optimized simultaneously.
2. carry out enlarged culturing again after bacillus amyloliquefaciens being carried out elementary cultivation, the growth of bacterial strain can be accelerated, avoid bacterial strain to assemble sedimentation, also reduce simultaneously and cause growing slack-off situation because of pH value change.
3. the mixing microorganisms zymolyte of bacillus amyloliquefaciens and the pig manure become thoroughly decomposed and the dish dregs of rice is mixed, effectively can solve the single strain growth of the zymocyte liquid nutrition problem slowly of single bacterial strain, bacterial strain can react with pig manure and the dish dregs of rice and improve the content of benefit materials simultaneously.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in further details.
The invention provides
a kind of have the microbial bacteria fertilizer improving soil acidity, comprise a kind of bacillus amyloliquefaciens with acid producing ability,
The first step, is inoculated into bacillus amyloliquefaciens in nutrient solution, carries out liquid fermenting life, working condition is, pH value is between 3.8 ~ 4.2, temperature 20 ~ 35 DEG C, fermentation time obtains original fermented solution in 5 ~ 7 days, wherein stirring velocity is 120 ~ 300 revs/min, and in described nutrient solution 1L, substances content is: the potato juice of 80 ~ 120g/L, yeast powder 0.5 ~ 1.5g, molasses 3 ~ 8g, KH2PO4 0.01g, sodium-chlor 0.5 ~ 1.5g, K2HPO4 0.01g; Bacterial strain WSA061 is carried out enlarged culturing, joining original fermented solution in the product ferment liquid of new configuration according to total amount 1:4, the greenhouse being placed in 25 ~ 40 DEG C carry out enlarged culturing 3 ~ 4 days can obtain zymocyte liquid;
Second step, by the mixing microorganisms zymolyte of the pig manure become thoroughly decomposed and the dish dregs of rice in mass ratio 7: 3 ratio mix and obtain coarse fodder; Then the zymocyte liquid the first step prepared is inoculated in coarse fodder by the amount of 60 ~ 180L/ ton and carries out solid fermentation, control temperature is below 55 DEG C, ferment 6 ~ 9 days, carry out turning 1 ~ 2 times/day in fermenting process, wherein after fermentation ends, bacterial strain WSA061 content reaches 1.5 × 10
8more than cfu/g, obtains microbial organic fertilizer;
Finally, be no more than the condition of 55 DEG C in temperature under, the water content of microbial organic fertilizer is evaporated to less than 30%, pack of finally packing.
The mixing microorganisms zymolyte preparation method of the described dish dregs of rice is: milk-acid bacteria, yeast, genus bacillus and Geotrichum are mixed according to 5:3:4:1, mixing microorganisms is added according to 1%--2% inoculum size in the dish dregs of rice, add the water of 55 ~ 65% of dish dregs of rice quality, obtain the mixing microorganisms zymolyte of the dish dregs of rice 15 ~ 20 DEG C of temperature bottom fermentation 48 hours fermentations.
The described pig manure germination index become thoroughly decomposed is more than 98%, and quality of organic matter is than content more than 45%, and organonitrogen quality is more than 1% than content, water content mass ratio 25-30%.Potted plant experiment:
(1) Lettuce potted plant experiment
1.1 use: mixing with soil uniformity, and original initial pH value is 8.0.Pot experiment does 4 process, 5 repetitions.I1: chemical fertilizer; I2: equivalent blank nutritive medium; I3: equivalent microbial organic fertilizer; CK: only water clear water and do not apply fertilizer.Above four other daily administrations of process are consistent.
1.2 results:
After surrounding, the pH value of the soil of I3 is reduced to 6.7 from 8.
I3 group is other contrast lifting capacitys (%) organized relatively:
| I1 | I2 | CK | |
| Soil urease activity | 37.58 | 27.54 | 42.48 |
| Chlorophyll content | / | / | 41.02 |
| Vc content | 35.21 | 33.57 | 42.01 |
| Output | 16.71 | 15.87 | 19.32 |
As can be seen from above-mentioned data, high by the Lettuce output of the microbial organic fertilizer plantation containing bacterial strain WSA061, the content of Vc content and soil urease activity only waters clear water apparently higher than I1 chemical fertilizer group, I2 equivalent blank nutritive medium group and CK and not to apply fertilizer group, and its Determination of Chlorophyll only waters clear water group of not applying fertilizer relative to CK obvious lifting.
(2) corn potted plant experiment
2.1 use: mixing with soil uniformity, and original initial pH value is 8.0.Field test does 4 process, and 3 are repeated Gong12Ge community.Treatments B 1: chemical fertilizer; Treatments B 2: the nutrient solution of equivalent; Treatments B 3: microbial organic fertilizer; Treatments B 4: clear water, other daily administrations are consistent.
2.2 results:
After surrounding, the pH value of the soil of B3 is reduced to 6.5 from 8.
B3 group is other contrast lifting capacitys (%) organized relatively:
| B1 | B2 | B4 | |
| Soil urease activity | 31.58 | 24.87 | 39.86 |
| Vc content | 26.31 | 25.21 | 37.41 |
| Output | 20.47 | 18.12 | 23.31 |
As can be seen from above-mentioned data, with the corn of the microbial organic fertilizer of planting containing bacterial strain WSA061 kind, the amplification of soil urease activity, chlorophyll content and Vc content is apparently higher than B2: the nutrient solution of equivalent, B3: chemical fertilizer, B4: clear water three groups.
The above; be only preferred embodiment of the present invention; not in order to limit the present invention, every above embodiment is done according to technical spirit of the present invention any trickle amendment, equivalently replace and improve, within the protection domain that all should be included in technical solution of the present invention.
Claims (3)
1. there is the microbial bacteria fertilizer improving soil acidity, it is characterized in that: comprise a kind of bacillus amyloliquefaciens with acid producing ability,
The first step, is inoculated into bacillus amyloliquefaciens in nutrient solution, carries out liquid fermenting life, working condition is, pH value is between 3.8 ~ 4.2, temperature 20 ~ 35 DEG C, fermentation time obtains original fermented solution in 5 ~ 7 days, wherein stirring velocity is 120 ~ 300 revs/min, and in described nutrient solution 1L, substances content is: the potato juice of 80 ~ 120g/L, yeast powder 0.5 ~ 1.5g, molasses 3 ~ 8g, KH2PO4 0.01g, sodium-chlor 0.5 ~ 1.5g, K2HPO4 0.01g; Bacterial strain WSA061 is carried out enlarged culturing, joining original fermented solution in the product ferment liquid of new configuration according to total amount 1:4, the greenhouse being placed in 25 ~ 40 DEG C carry out enlarged culturing 3 ~ 4 days can obtain zymocyte liquid;
Second step, by the mixing microorganisms zymolyte of the pig manure become thoroughly decomposed and the dish dregs of rice in mass ratio 7: 3 ratio mix and obtain coarse fodder; Then the zymocyte liquid the first step prepared is inoculated in coarse fodder by the amount of 60 ~ 180L/ ton and carries out solid fermentation, control temperature is below 55 DEG C, ferment 6 ~ 9 days, carry out turning 1 ~ 2 times/day in fermenting process, wherein after fermentation ends, bacterial strain WSA061 content reaches 1.5 × 10
8more than cfu/g, obtains microbial organic fertilizer;
Finally, be no more than the condition of 55 DEG C in temperature under, the water content of microbial organic fertilizer is evaporated to less than 30%, pack of finally packing.
2. having described in 1 to be gone to improve the microbial bacteria fertilizer of soil acidity according to right, it is characterized in that: the mixing microorganisms zymolyte preparation method of the described dish dregs of rice is: milk-acid bacteria, yeast, genus bacillus and Geotrichum are mixed according to 5:3:4:1, mixing microorganisms is added according to 1%--2% inoculum size in the dish dregs of rice, add the water of 55 ~ 65% of dish dregs of rice quality, obtain the mixing microorganisms zymolyte of the dish dregs of rice 15 ~ 20 DEG C of temperature bottom fermentation 48 hours fermentations.
3. having described in 1 to be gone to improve the microbial bacteria fertilizer of soil acidity according to right, it is characterized in that: described in the pig manure germination index that becomes thoroughly decomposed be more than 98%, quality of organic matter is than content more than 45%, and organonitrogen quality is more than 1% than content, water content mass ratio 25-30%.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106590671A (en) * | 2016-11-02 | 2017-04-26 | 湖北富之源生物科技有限公司 | Soil conditioner and preparation method and applications thereof |
| CN114456014A (en) * | 2022-02-09 | 2022-05-10 | 广东森度生态农业科技有限公司 | Anti-hardening microbial fermentation type organic fertilizer and preparation method thereof |
Citations (3)
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|---|---|---|---|---|
| CN102531801A (en) * | 2012-01-05 | 2012-07-04 | 上海创博生态工程有限公司 | Acidic microbial organic fertilizer and preparation method thereof |
| CN103173387A (en) * | 2013-03-14 | 2013-06-26 | 南京农业大学 | Growth-promoting bacteria for facilitating growth of rape and microbial organic fertilizer |
| CN104498040A (en) * | 2014-11-10 | 2015-04-08 | 杨凌绿都生物科技有限公司 | Application of Bacillus amyloliquefaciens as soil conditioner |
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2015
- 2015-04-14 CN CN201510179533.5A patent/CN104860733A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102531801A (en) * | 2012-01-05 | 2012-07-04 | 上海创博生态工程有限公司 | Acidic microbial organic fertilizer and preparation method thereof |
| CN103173387A (en) * | 2013-03-14 | 2013-06-26 | 南京农业大学 | Growth-promoting bacteria for facilitating growth of rape and microbial organic fertilizer |
| CN104498040A (en) * | 2014-11-10 | 2015-04-08 | 杨凌绿都生物科技有限公司 | Application of Bacillus amyloliquefaciens as soil conditioner |
Non-Patent Citations (2)
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| 钱爱东: "《食品微生物学 第二版》", 31 March 2008 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106590671A (en) * | 2016-11-02 | 2017-04-26 | 湖北富之源生物科技有限公司 | Soil conditioner and preparation method and applications thereof |
| CN106590671B (en) * | 2016-11-02 | 2022-01-18 | 湖北富之源生物科技有限公司 | Soil conditioner and preparation method and application thereof |
| CN114456014A (en) * | 2022-02-09 | 2022-05-10 | 广东森度生态农业科技有限公司 | Anti-hardening microbial fermentation type organic fertilizer and preparation method thereof |
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Application publication date: 20150826 |